Standard name | |
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Human Ortholog | |
Description | Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 5 | 10 |
Bud | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 2 | 5 | – | 7 | 7 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 10 | 22 |
Bud Neck | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 0 | – | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 3 | 1 | 1 | 0 | 2 | 2 | 4 | – | 0 | 2 | 1 | 1 | 1 | 8 | 3 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Cytoplasm | 105 | 110 | 195 | 133 | 179 | 194 | 297 | 317 | 222 | – | 146 | 191 | 379 | 352 | 448 | 100 | 98 | 59 | 115 | 119 | 179 | 171 | 347 | 261 |
Endoplasmic Reticulum | 0 | 2 | 3 | 0 | 1 | 1 | 1 | 1 | 2 | – | 1 | 2 | 0 | 0 | 1 | 7 | 9 | 2 | 0 | 0 | 2 | 5 | 6 | 5 |
Endosome | 1 | 0 | 5 | 0 | 4 | 5 | 3 | 4 | 0 | – | 0 | 0 | 0 | 4 | 0 | 2 | 4 | 3 | 2 | 2 | 6 | 0 | 12 | 14 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 1 | 0 | 0 | 0 | 5 | 3 |
Mitochondria | 2 | 2 | 1 | 6 | 4 | 19 | 46 | 26 | 89 | – | 94 | 76 | 3 | 0 | 2 | 4 | 2 | 2 | 2 | 1 | 9 | 13 | 41 | 35 |
Nucleus | 1 | 0 | 2 | 1 | 2 | 2 | 3 | 3 | 1 | – | 0 | 8 | 1 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
SpindlePole | 0 | 0 | 3 | 0 | 2 | 1 | 3 | 0 | 2 | – | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 3 | 0 | 9 | 8 |
Vac/Vac Membrane | 1 | 2 | 4 | 1 | 1 | 4 | 1 | 6 | 5 | – | 4 | 3 | 2 | 1 | 2 | 6 | 4 | 3 | 2 | 0 | 3 | 9 | 18 | 16 |
Unique Cell Count | 109 | 114 | 207 | 133 | 191 | 210 | 321 | 343 | 269 | 200 | 249 | 381 | 356 | 453 | 111 | 110 | 67 | 129 | 126 | 218 | 210 | 470 | 390 | |
Labelled Cell Count | 110 | 117 | 218 | 145 | 194 | 228 | 359 | 363 | 331 | 253 | 295 | 387 | 359 | 456 | 134 | 125 | 76 | 129 | 126 | 218 | 210 | 470 | 390 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.0 | 5.7 | 5.1 | 5.5 | 4.5 | 4.6 | 5.0 | 4.2 | – | 3.8 | 4.1 | 5.4 | 5.4 | 5.4 | 9.1 | 9.9 | 8.5 | 5.2 | 4.9 | 5.3 |
Std Deviation (1e-4) | 1.0 | 0.8 | 1.1 | 1.6 | 1.2 | 1.0 | 1.3 | 1.6 | 1.7 | – | 1.0 | 1.3 | 0.9 | 1.3 | 1.5 | 2.3 | 2.7 | 1.9 | 1.8 | 1.5 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.06 | -0.35 | -0.33 | -0.2 | -0.44 | – | -0.6 | -0.47 | -0.1 | -0.09 | -0.09 | 0.66 | 0.79 | 0.56 | -0.15 | -0.23 | -0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.8 | -0.2 | -0.7 | -0.7 | -0.8 | -3.8 | – | -5.8 | -5.2 | 4.0 | 3.2 | 3.5 | -1.3 | -1.6 | -1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 3.7 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.9153 | 2.9425 | 2.6653 | 2.1506 | 1.8444 | 2.7591 | 0.1786 | 1.3754 | 1.0833 | 0.9818 | 0.5236 | 0.9422 | 1.4052 | 2.2476 | 1.7737 | 1.8513 | 1.8882 | 1.6853 |
Actin | 0.0275 | 0.0013 | 0.0112 | 0.0013 | 0.0003 | 0.0021 | 0.0085 | 0.0007 | 0.0091 | 0.04 | 0.0008 | 0.0009 | 0.0301 | 0.0071 | 0.0118 | 0.0114 | 0.0216 | 0.0108 |
Bud | 0.001 | 0.0007 | 0.0014 | 0.0001 | 0.0001 | 0.0015 | 0.0014 | 0.0039 | 0.0007 | 0.026 | 0.0001 | 0.0028 | 0.0145 | 0.0264 | 0.0273 | 0.0115 | 0.024 | 0.0148 |
Bud Neck | 0.0009 | 0.0004 | 0.0005 | 0.0011 | 0.0002 | 0.0039 | 0.0019 | 0.0003 | 0.0005 | 0.001 | 0.0002 | 0.0033 | 0.0015 | 0.0011 | 0.0013 | 0.0039 | 0.0012 | 0.0037 |
Bud Periphery | 0.0049 | 0.0005 | 0.0015 | 0.0001 | 0.0001 | 0.0064 | 0.0014 | 0.0048 | 0.0006 | 0.0142 | 0.0001 | 0.0021 | 0.0097 | 0.0123 | 0.0156 | 0.0097 | 0.0192 | 0.0119 |
Bud Site | 0.0017 | 0.0069 | 0.0016 | 0.0002 | 0.0001 | 0.0013 | 0.0091 | 0.0136 | 0.0045 | 0.0036 | 0 | 0.0004 | 0.0284 | 0.0914 | 0.0346 | 0.0091 | 0.003 | 0.0019 |
Cell Periphery | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0016 | 0.0016 | 0.0014 | 0.0008 | 0.0006 | 0.0005 |
Cytoplasm | 0.286 | 0.4727 | 0.451 | 0.5474 | 0.4671 | 0.5841 | 0.4622 | 0.76 | 0.6819 | 0.5955 | 0.7108 | 0.7835 | 0.344 | 0.4931 | 0.4397 | 0.4254 | 0.5146 | 0.5548 |
Cytoplasmic Foci | 0.0209 | 0.0119 | 0.0099 | 0.0117 | 0.0085 | 0.0067 | 0.0461 | 0.0113 | 0.0218 | 0.0347 | 0.0136 | 0.0152 | 0.0518 | 0.0182 | 0.0275 | 0.0206 | 0.0211 | 0.0297 |
Eisosomes | 0.0008 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0007 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.011 | 0.0045 | 0.0051 | 0.0046 | 0.0014 | 0.0034 | 0.0035 | 0.0022 | 0.0032 | 0.0023 | 0.0054 | 0.0021 | 0.0127 | 0.0025 | 0.0059 | 0.0066 | 0.0076 | 0.0055 |
Endosome | 0.0095 | 0.0063 | 0.0051 | 0.0079 | 0.0015 | 0.0045 | 0.049 | 0.0038 | 0.016 | 0.0526 | 0.0101 | 0.0087 | 0.0245 | 0.0024 | 0.0082 | 0.0312 | 0.0114 | 0.0188 |
Golgi | 0.003 | 0.0006 | 0.0007 | 0.0005 | 0.0001 | 0.0006 | 0.0168 | 0.0003 | 0.0106 | 0.0391 | 0.0004 | 0.0003 | 0.0117 | 0.0014 | 0.003 | 0.0122 | 0.0056 | 0.0054 |
Lipid Particles | 0.0064 | 0.0005 | 0.0019 | 0.0023 | 0.0009 | 0.0003 | 0.0263 | 0.0001 | 0.0027 | 0.0047 | 0.0001 | 0.0001 | 0.0103 | 0.0008 | 0.005 | 0.0132 | 0.0094 | 0.0092 |
Mitochondria | 0.0027 | 0.0006 | 0.0006 | 0.0008 | 0.0005 | 0.0013 | 0.0126 | 0.0003 | 0.0092 | 0.0052 | 0.0003 | 0.0003 | 0.0129 | 0.0016 | 0.0101 | 0.0096 | 0.0297 | 0.0052 |
None | 0.5921 | 0.4773 | 0.4953 | 0.409 | 0.5149 | 0.377 | 0.3167 | 0.192 | 0.2284 | 0.1575 | 0.2524 | 0.1744 | 0.3987 | 0.3042 | 0.3536 | 0.3953 | 0.2789 | 0.2759 |
Nuclear Periphery | 0.0035 | 0.0021 | 0.0016 | 0.0022 | 0.0004 | 0.0011 | 0.0034 | 0.0006 | 0.0011 | 0.0017 | 0.0012 | 0.0008 | 0.0069 | 0.0021 | 0.006 | 0.005 | 0.0153 | 0.0069 |
Nucleolus | 0.0004 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0025 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0033 | 0.0025 | 0.0024 | 0.0011 | 0.0012 | 0.0015 |
Nucleus | 0.0022 | 0.0048 | 0.003 | 0.0068 | 0.0011 | 0.0025 | 0.0027 | 0.0026 | 0.0021 | 0.0067 | 0.0011 | 0.0021 | 0.0076 | 0.0096 | 0.0151 | 0.0138 | 0.016 | 0.0173 |
Peroxisomes | 0.007 | 0.0005 | 0.0045 | 0.0003 | 0.0002 | 0.0003 | 0.0215 | 0.0004 | 0.0041 | 0.0066 | 0.0001 | 0.0002 | 0.0092 | 0.0014 | 0.0065 | 0.0015 | 0.002 | 0.0011 |
Punctate Nuclear | 0.0156 | 0.0039 | 0.0015 | 0.0013 | 0.0006 | 0.0006 | 0.0075 | 0.0011 | 0.0009 | 0.0016 | 0.0004 | 0.0009 | 0.0134 | 0.017 | 0.0198 | 0.011 | 0.0113 | 0.021 |
Vacuole | 0.0019 | 0.0031 | 0.0024 | 0.0015 | 0.001 | 0.0016 | 0.0044 | 0.0015 | 0.0016 | 0.0046 | 0.0023 | 0.0015 | 0.0052 | 0.0025 | 0.0041 | 0.0055 | 0.0038 | 0.0026 |
Vacuole Periphery | 0.0006 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0016 | 0.0002 | 0.0004 | 0.0018 | 0.0005 | 0.0003 | 0.0013 | 0.0003 | 0.0009 | 0.0015 | 0.0022 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.9179 | 17.9545 | 19.4294 | 26.6718 | 21.1581 | 28.8558 | 19.0993 | 22.9736 | 22.052 | 25.0711 |
Translational Efficiency | 0.9694 | 1.0177 | 0.907 | 0.7102 | 0.7471 | 0.8527 | 0.8209 | 0.5992 | 0.6984 | 0.6232 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1941 | 195 | 1225 | 1677 | 1660 | 1431 | 1789 | 204 | 3601 | 1626 | 3014 | 1881 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 687.10 | 852.93 | 1030.22 | 1033.49 | 683.40 | 808.46 | 999.27 | 1011.15 | 685.39 | 813.79 | 1011.85 | 1031.07 |
Standard Deviation | 75.78 | 125.11 | 127.90 | 137.85 | 97.81 | 105.42 | 130.27 | 148.54 | 86.66 | 108.93 | 130.20 | 139.22 |
Intensity Change Log 2 | — | 0.311907 | 0.584360 | 0.588932 | — | 0.242446 | 0.548144 | 0.565195 | — | 0.277688 | 0.566415 | 0.577145 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000081 | 0.000763 | 0.001783 | 0.001725 | 0.000138 | 0.000929 | 0.000341 | 0.002988 | 0.000107 | 0.000909 | 0.000927 | 0.001862 |
Bud Neck | 0.010526 | 0.014718 | 0.006288 | 0.012410 | 0.006862 | 0.023772 | 0.003099 | 0.006512 | 0.008837 | 0.022686 | 0.004395 | 0.011770 |
Bud Site | 0.004666 | 0.019911 | 0.014827 | 0.050961 | 0.003596 | 0.018008 | 0.006163 | 0.091634 | 0.004173 | 0.018236 | 0.009685 | 0.055372 |
Cell Periphery | 0.000101 | 0.000160 | 0.000133 | 0.000153 | 0.000097 | 0.000155 | 0.000107 | 0.000115 | 0.000099 | 0.000156 | 0.000118 | 0.000149 |
Cytoplasm | 0.602520* | 0.454589* | 0.431213* | 0.433214* | 0.580454* | 0.447657* | 0.530836* | 0.183645* | 0.592348* | 0.448488* | 0.490346* | 0.406148* |
Cytoplasmic Foci | 0.173957 | 0.268608* | 0.008143 | 0.019071 | 0.186614* | 0.253384* | 0.007164 | 0.041742 | 0.179792 | 0.255210* | 0.007562 | 0.021529 |
Eisosomes | 0.000109 | 0.000131 | 0.000028 | 0.000030 | 0.000083 | 0.000123 | 0.000021 | 0.000041 | 0.000097 | 0.000124 | 0.000024 | 0.000031 |
Endoplasmic Reticulum | 0.000640 | 0.000772 | 0.004486 | 0.002206 | 0.000913 | 0.000803 | 0.002673 | 0.001294 | 0.000766 | 0.000800 | 0.003410 | 0.002107 |
Endosome | 0.008355 | 0.035173 | 0.002358 | 0.009119 | 0.010163 | 0.035712 | 0.001363 | 0.007704 | 0.009188 | 0.035647 | 0.001768 | 0.008965 |
Golgi | 0.001532 | 0.002664 | 0.000110 | 0.006233 | 0.001392 | 0.007977 | 0.000082 | 0.000947 | 0.001468 | 0.007340 | 0.000094 | 0.005660 |
Lipid Particles | 0.004223 | 0.003436 | 0.001387 | 0.001151 | 0.006232 | 0.006020 | 0.000772 | 0.001752 | 0.005149 | 0.005710 | 0.001022 | 0.001216 |
Mitochondria | 0.004226 | 0.001616 | 0.000684 | 0.005751 | 0.002707 | 0.007493 | 0.000741 | 0.000157 | 0.003526 | 0.006788 | 0.000717 | 0.005144 |
Mitotic Spindle | 0.000560 | 0.008983 | 0.004583 | 0.191878* | 0.002456 | 0.010141 | 0.024256 | 0.491301* | 0.001434 | 0.010002 | 0.016260 | 0.224352* |
None | 0.004906 | 0.003179 | 0.005161 | 0.006570 | 0.005999 | 0.002617 | 0.005772 | 0.004517 | 0.005410 | 0.002685 | 0.005524 | 0.006347 |
Nuclear Periphery | 0.000241 | 0.000242 | 0.002470 | 0.002327 | 0.000402 | 0.000330 | 0.001712 | 0.000741 | 0.000315 | 0.000319 | 0.002020 | 0.002155 |
Nuclear Periphery Foci | 0.000362 | 0.001395 | 0.002806 | 0.004160 | 0.000820 | 0.000502 | 0.002044 | 0.007840 | 0.000574 | 0.000609 | 0.002354 | 0.004559 |
Nucleolus | 0.000906 | 0.000576 | 0.000297 | 0.000805 | 0.001384 | 0.001358 | 0.000304 | 0.001235 | 0.001126 | 0.001265 | 0.000301 | 0.000851 |
Nucleus | 0.112427 | 0.052392 | 0.444027* | 0.157766* | 0.132346* | 0.052702 | 0.354733* | 0.029146 | 0.121609 | 0.052665 | 0.391026* | 0.143817* |
Peroxisomes | 0.001941 | 0.010526 | 0.000294 | 0.003327 | 0.002063 | 0.010773 | 0.000392 | 0.015928 | 0.001997 | 0.010743 | 0.000352 | 0.004693 |
Vacuole | 0.066854 | 0.118101 | 0.068729 | 0.085604 | 0.054368 | 0.116226 | 0.057307 | 0.110596 | 0.061098 | 0.116451 | 0.061949 | 0.088315 |
Vacuole Periphery | 0.000864 | 0.002063 | 0.000192 | 0.005539 | 0.000912 | 0.003318 | 0.000118 | 0.000165 | 0.000886 | 0.003167 | 0.000148 | 0.004957 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.29 | -1.81 | -6.05 | -0.51 | 0.44 | -1.72 | -5.48 | -1.77 | -0.53 | -1.40 | -1.97 | -2.45 | -5.92 | -0.14 | -0.85 |
Bud Neck | -1.07 | 3.15 | -2.30 | 0.26 | -5.07 | -10.03 | 2.03 | -2.33 | 6.72 | -3.64 | -9.12 | 4.34 | -4.41 | 6.14 | -7.95 |
Bud Site | -4.47 | -3.82 | -11.16 | -1.80 | -5.33 | -9.53 | -3.75 | -5.76 | -4.28 | -5.39 | -10.28 | -5.16 | -12.69 | -4.96 | -9.44 |
Cell Periphery | -2.08 | -5.77 | -2.09 | 1.10 | 1.36 | -3.91 | -2.05 | -0.93 | 2.75 | 0.36 | -4.34 | -5.16 | -2.30 | 1.94 | 0.09 |
Cytoplasm | 7.46 | 29.10 | 29.79 | 5.06 | -0.53 | 13.02 | 17.60 | 26.18 | 17.57 | 16.37 | 17.32 | 32.04 | 35.16 | 13.02 | 7.85 |
Cytoplasmic Foci | -5.68 | 41.55 | 37.34 | 16.33 | -10.19 | -8.90 | 38.02 | 19.65 | 26.16 | -4.34 | -12.07 | 56.24 | 48.40 | 43.43 | -11.20 |
Eisosomes | -0.65 | 9.76 | 10.25 | 2.94 | 1.29 | -3.92 | 6.13 | 2.44 | 7.63 | -4.30 | -3.14 | 11.46 | 10.53 | 12.61 | -2.00 |
Endoplasmic Reticulum | -1.49 | -21.10 | -11.15 | -7.80 | 14.44 | 0.88 | -12.22 | -4.99 | -5.42 | 3.39 | -0.47 | -23.06 | -11.53 | -10.64 | 12.85 |
Endosome | -5.45 | 8.92 | 4.73 | 6.26 | -3.97 | -13.91 | 11.42 | -0.15 | 12.12 | -7.14 | -16.03 | 14.71 | 6.03 | 18.57 | -7.01 |
Golgi | -1.54 | 10.06 | 0.17 | 1.36 | -2.76 | -7.14 | 9.26 | 3.25 | 7.82 | -5.64 | -7.20 | 13.67 | 0.20 | 6.48 | -2.95 |
Lipid Particles | 1.06 | 7.32 | 6.11 | 1.96 | -3.15 | 0.32 | 8.24 | 3.27 | 3.40 | -4.72 | -1.03 | 11.12 | 8.25 | 7.93 | -7.75 |
Mitochondria | 1.78 | 3.16 | 1.89 | -0.35 | -2.08 | -4.26 | 2.84 | 3.59 | 8.01 | 7.48 | -3.10 | 4.12 | 2.16 | 5.34 | -1.95 |
Mitotic Spindle | -2.01 | -5.63 | -24.05 | -19.78 | -23.20* | -3.78 | -8.96 | -12.46 | -12.17 | -11.36 | -4.96 | -10.07 | -26.58 | -24.91 | -23.28 |
None | 0.99 | 1.32 | -6.17 | -3.41 | -9.71 | 3.12 | 0.02 | 0.52 | -1.36 | 0.56 | 3.48 | 0.35 | -5.17 | -8.05 | -7.23 |
Nuclear Periphery | -0.55 | -27.44 | -14.32 | -14.21 | -0.24 | 1.14 | -23.23 | -9.08 | -9.45 | 1.97 | -0.35 | -35.85 | -14.98 | -14.85 | -1.37 |
Nuclear Periphery Foci | -3.80 | -13.09 | -11.02 | -8.54 | -6.05 | 2.45 | -5.74 | -5.25 | -5.33 | -4.33 | -0.45 | -9.23 | -11.69 | -11.64 | -6.48 |
Nucleolus | 1.56 | 2.81 | -5.89 | -6.66 | -7.09 | 0.20 | 1.30 | -1.61 | -1.83 | -2.50 | -0.45 | 2.02 | -5.78 | -5.59 | -6.58 |
Nucleus | 9.49 | -43.33 | -13.37 | -17.95 | 29.60 | 18.20 | -34.46 | 8.22 | -0.63 | 31.65 | 22.35 | -54.71 | -10.22 | -23.20 | 35.01 |
Peroxisomes | -3.71 | 10.19 | 0.37 | 3.73 | -4.30 | -8.80 | 9.39 | -2.18 | 0.38 | -2.70 | -9.59 | 13.62 | -1.23 | 7.83 | -4.79 |
Vacuole | -5.52 | -11.24 | -18.28 | -3.51 | -5.63 | -13.13 | -13.29 | -9.52 | -6.50 | -6.44 | -13.66 | -17.04 | -21.66 | -9.62 | -9.37 |
Vacuole Periphery | -1.10 | 5.92 | -0.82 | 0.74 | -2.68 | -8.31 | 6.88 | 6.06 | 11.68 | -4.59 | -8.28 | 9.10 | -0.55 | 4.97 | -2.82 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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