Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0
Bud 0 0 0.07 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.06 0.12 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.06 0.1 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.11 0.08 0.11 0.05 0 0.08 0 0 0
Golgi 0.05 0.12 0 0 0 0 0 0 0 0 0 0.15 0.13 0.27 0.3 0.29 0.24 0.07 0.11 0 0.11 0.12 0.14
Mitochondria 0.91 0.82 0.95 0.96 0.92 0.92 0.94 0.96 0.96 0.96 0.94 0.89 0.79 0.74 0.1 0.05 0.1 0.77 0.73 0.77 0.66 0.61 0.66
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.09 0.09 0.11 0.07 0.07 0.09 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0.07 0.17 0.1 0.1 0.11 0.08 0 0 0.05 0.09 0.11 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.17 0.05 0 0.06 0 0 0 0 0 0 0.07 0.08 0.09 0.16 0.14 0.16 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 5 3 1 0 0 0 0 1 3 0 2 0 1 4 7 7 16 17 3 10 24 17
Bud 14 3 35 19 27 11 29 14 23 18 20 15 5 3 7 16 9 1 3 0 2 1 1
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1
Bud Site 1 0 2 2 8 3 6 7 4 8 7 2 1 0 1 2 1
Cell Periphery 24 35 13 15 5 5 3 5 9 1 15 14 19 32 13 8 25 0 1 0 0 1 0
Cytoplasm 5 6 11 13 24 23 29 16 17 8 14 7 10 18 9 15 26 0 3 1 0 1 0
Endoplasmic Reticulum 6 5 7 5 5 5 6 5 10 7 9 5 1 3 4 5 10 1 3 0 0 4 3
Endosome 12 8 1 0 0 5 3 0 3 0 0 9 43 34 18 11 24 41 17 42 18 20 16
Golgi 36 67 1 1 0 1 2 0 0 0 1 72 71 155 48 42 50 53 64 12 44 57 66
Mitochondria 697 442 483 381 378 578 908 696 770 492 507 425 423 428 16 7 21 614 448 390 266 295 321
Nucleus 2 5 7 1 7 4 4 3 2 2 2 3 1 1 5 6 6 0 0 0 1 0 0
Nuclear Periphery 25 18 19 17 9 56 92 79 57 38 51 19 9 2 8 4 10 6 3 2 6 1 3
Nucleolus 20 7 10 7 14 33 65 36 36 22 19 8 5 2 6 4 4 3 2 0 1 3 1
Peroxisomes 6 12 2 4 1 1 8 2 5 0 5 35 90 56 16 16 18 17 19 27 36 54 35
SpindlePole 1 3 7 0 4 2 14 5 6 1 3 2 6 11 8 8 18 3 1 4 3 2 1
Vac/Vac Membrane 108 91 25 7 24 25 26 31 26 15 13 33 43 52 25 21 34 35 21 8 11 10 10
Unique Cell Count 762 539 506 397 410 627 971 725 804 515 537 478 534 582 158 145 212 801 611 505 407 484 484
Labelled Cell Count 964 707 627 473 506 752 1195 899 969 615 666 651 727 798 188 172 263 801 611 505 407 484 484


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 8.7 4.6 5.3 3.9 5.5 5.5 5.1 5.6 5.4 5.4 7.7 8.5 8.2 8.5 8.2 7.8 8.4 8.9 9.2
Std Deviation (1e-4) 1.2 1.4 1.0 1.2 1.0 1.4 1.3 1.3 1.3 1.4 1.4 1.8 1.5 1.6 1.8 2.0 1.8 1.5 1.7 1.8
Intensity Change (Log2) 0.21 -0.22 0.25 0.26 0.14 0.29 0.24 0.23 0.75 0.88 0.84 0.88 0.83 0.76 0.87 0.96 1.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -1.3 0 -4.4 -3.5 -4.4 -3.5 -2.5 -2.3 -2.7 -5.0 -5.7 -1.1 1.6 -1.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.0 0 -2.4 -4.0 -2.7 -2.0 -3.3 0.2 0.3 0.9 2.4 3.2 0 5.0
Cytoplasm 1.0 2.9 1.5 0.9 0 -0.1 -0.7 0.5 -0.8 -0.3 0.9 0 4.4 5.6
Endoplasmic Reticulum -0.2 0 -1.0 0 0 -0.2 0 0.4 -0.5 0 0 0 0 2.7
Endosome 0 0 0 0 0 0 0 0 0 6.3 5.3 0 0 7.4
Golgi 0 0 0 0 0 0 0 0 8.9 8.3 12.4 12.7 12.3 11.1
Mitochondria 0.4 0 -2.2 -1.5 0.5 0.3 0.1 -0.8 -3.8 -7.8 -9.8 -21.7 -22.3 -22.9
Nucleus 0 0.4 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.4 0 3.5 4.0 4.6 2.5 2.5 3.7 0.2 -2.1 -4.1 0.7 -0.6 0.6
Nucleolus -0.2 0 2.9 3.9 2.7 2.4 2.1 1.5 -0.4 -1.4 -2.6 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 5.7 9.3 6.8 0 0 6.0
SpindlePole 0 0 0 0.1 0 -1.1 0 0 0 -0.4 0.7 0 0 4.7
Vacuole -2.6 0.6 -0.8 -2.3 -0.6 -1.6 -1.7 -2.2 1.3 2.0 2.6 4.5 4.0 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.1952 6.6718 5.9825 5.805 6.2112 6.5348 5.1158 5.0637 5.0368 4.3002 4.778 5.0428 7.7895 7.1941 7.6292 7.5697 7.2013 7.3001
Actin 0.024 0.0064 0.0159 0.0174 0.0003 0.0092 0.008 0.0043 0.0056 0.001 0.0154 0.0029 0.0039 0.0025 0.0013 0.0005 0.0016 0.0012
Bud 0.0008 0.0003 0.0012 0.0002 0 0.0001 0.0002 0.0002 0.0001 0 0.0003 0 0.0003 0.0003 0.0003 0.0003 0.0032 0
Bud Neck 0.0038 0.0001 0.0003 0.0004 0 0.0001 0.0002 0.0001 0.0001 0 0.0005 0.0001 0.0002 0.0001 0.0001 0 0 0
Bud Periphery 0.0016 0.0006 0.0017 0.0004 0.0001 0.0002 0.0009 0.0003 0.0002 0.0001 0.0008 0.0001 0.0008 0.0006 0.0011 0.0022 0.0216 0.0001
Bud Site 0.0058 0.0005 0.0108 0.0011 0 0.0001 0.0004 0.0003 0.0005 0.0001 0.0003 0.0001 0.0005 0.0014 0.0005 0.0001 0.0002 0
Cell Periphery 0.0007 0.0003 0.0004 0.0001 0 0.0001 0.0003 0.0001 0.0001 0 0.0002 0 0.0004 0.0004 0.0002 0.0002 0.0005 0
Cytoplasm 0.0026 0.0001 0.001 0.0003 0 0 0.0003 0.0001 0 0 0.0002 0 0.0003 0.0001 0.0001 0 0.0001 0
Cytoplasmic Foci 0.0139 0.0016 0.0089 0.0109 0.0031 0.002 0.008 0.0025 0.0017 0.0007 0.0056 0.0012 0.0027 0.005 0.0017 0.0009 0.001 0.0007
Eisosomes 0.0004 0.0002 0.0002 0.0002 0.0001 0.0001 0.0003 0.0002 0.0002 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0014 0.0001 0.0005 0.0002 0 0.0002 0.0003 0 0.0001 0 0.0005 0.0001 0.0007 0.0001 0.0001 0 0.0001 0.0001
Endosome 0.0207 0.0135 0.018 0.0245 0.0212 0.007 0.0173 0.0098 0.0034 0.0011 0.0387 0.0035 0.0118 0.0101 0.007 0.0041 0.0053 0.0066
Golgi 0.0314 0.0136 0.0193 0.0297 0.0269 0.0152 0.0226 0.0201 0.0094 0.0064 0.0495 0.011 0.0154 0.0173 0.0117 0.0188 0.012 0.0209
Lipid Particles 0.0385 0.0123 0.0077 0.019 0.0017 0.0118 0.0166 0.0059 0.0061 0.0011 0.0318 0.0047 0.0156 0.0108 0.0026 0.0018 0.0015 0.0032
Mitochondria 0.8095 0.9212 0.8861 0.8768 0.9413 0.9378 0.9017 0.9454 0.9565 0.9852 0.8252 0.9649 0.9312 0.9286 0.961 0.9635 0.9367 0.9632
None 0.0006 0 0.0002 0.0002 0 0.0001 0.0005 0 0 0 0.0002 0 0.0006 0 0 0.0001 0.0001 0
Nuclear Periphery 0.011 0.0001 0.0003 0.0002 0 0.0004 0.0026 0 0.0002 0 0.0007 0.0001 0.0016 0.0001 0 0 0.0001 0
Nucleolus 0.0032 0.0003 0.0004 0.0001 0 0.0001 0.0006 0.0001 0 0 0.0002 0 0.0002 0.0002 0.0001 0 0 0
Nucleus 0.0041 0.0002 0.0009 0.0001 0 0.0001 0.0005 0 0 0 0.0002 0 0.0004 0.0001 0.0001 0 0 0
Peroxisomes 0.0102 0.0053 0.0103 0.0136 0.0026 0.0118 0.0133 0.0085 0.0133 0.0033 0.0037 0.0097 0.0058 0.0164 0.0088 0.0053 0.0093 0.0016
Punctate Nuclear 0.0028 0 0.0003 0.0007 0 0.0005 0.0004 0 0 0 0.0003 0.0001 0.0005 0 0 0 0 0
Vacuole 0.0078 0.014 0.0057 0.0012 0.0006 0.0007 0.0019 0.0007 0.0004 0.0001 0.0122 0.0003 0.0027 0.0023 0.002 0.0007 0.0013 0.0004
Vacuole Periphery 0.0053 0.0093 0.0099 0.0026 0.002 0.0024 0.003 0.0014 0.0019 0.0005 0.0133 0.001 0.0042 0.0033 0.0013 0.0012 0.0053 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.7654 15.6568 13.2777 28.9975 19.4641 17.5047 28.2688 37.3959 25.0976 36.7139
Translational Efficiency 3.4958 3.4937 3.7989 1.5333 3.0464 3.9873 2.3282 1.5999 2.5514 1.8681

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1071 1380 68 222 1857 1862 1248 1233 2928 3242 1316 1455

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 823.57 900.60 1152.33 1208.51 729.86 895.42 1039.75 1075.74 764.14 897.62 1045.57 1096.00
Standard Deviation 136.21 157.58 158.20 160.71 129.61 146.11 156.76 164.13 139.56 151.12 158.80 170.43
Intensity Change Log 2 0.128995 0.484591 0.553266 0.294945 0.510545 0.559638 0.209346 0.496843 0.556263

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002059 0.001637 0.002491 0.012736 0.002148 0.004807 0.005374 0.010763 0.002115 0.003458 0.005225 0.011064
Bud Neck 0.002407 0.000758 0.008095 0.002659 0.001201 0.000674 0.003758 0.002265 0.001642 0.000710 0.003982 0.002325
Bud Site 0.003955 0.001651 0.004277 0.008996 0.001953 0.001596 0.012555 0.012980 0.002685 0.001620 0.012127 0.012372
Cell Periphery 0.000591 0.000193 0.000244 0.000408 0.000585 0.000329 0.000263 0.000475 0.000587 0.000271 0.000262 0.000465
Cytoplasm 0.001166 0.000478 0.001278 0.001027 0.000346 0.000410 0.001687 0.001477 0.000646 0.000439 0.001666 0.001408
Cytoplasmic Foci 0.023699 0.021925 0.093262 0.036475 0.005964 0.012345 0.018539 0.015238 0.012451 0.016423 0.022400 0.018478
Eisosomes 0.000056 0.000049 0.000083 0.000133 0.000048 0.000067 0.000063 0.000112 0.000051 0.000059 0.000064 0.000116
Endoplasmic Reticulum 0.000341 0.000153 0.003297 0.001411 0.000195 0.000168 0.000360 0.000485 0.000248 0.000162 0.000512 0.000627
Endosome 0.049904 0.032970 0.086296 0.031783 0.011432 0.023271 0.033145 0.020772 0.025504 0.027400 0.035891 0.022452
Golgi 0.036124 0.036622 0.067819 0.108725 0.013226 0.023788 0.030817 0.046666 0.021601 0.029251 0.032729 0.056135
Lipid Particles 0.007821 0.003078 0.017434 0.002094 0.002561 0.001800 0.002517 0.000887 0.004485 0.002344 0.003287 0.001071
Mitochondria 0.820461* 0.869006* 0.523152* 0.678692* 0.940881* 0.902721* 0.820241* 0.841577* 0.896834* 0.888370* 0.804890* 0.816724*
Mitotic Spindle 0.005384 0.002803 0.090993 0.034975 0.001041 0.002093 0.026397 0.008732 0.002629 0.002395 0.029735 0.012736
None 0.000271 0.000209 0.000809 0.000505 0.000201 0.000151 0.000323 0.000278 0.000226 0.000176 0.000348 0.000313
Nuclear Periphery 0.000250 0.000138 0.011870 0.001144 0.000059 0.000053 0.000404 0.000345 0.000129 0.000089 0.000996 0.000467
Nuclear Periphery Foci 0.000479 0.000179 0.011112 0.000913 0.000094 0.000076 0.000411 0.000324 0.000235 0.000120 0.000964 0.000414
Nucleolus 0.000682 0.000086 0.001472 0.000188 0.000213 0.000039 0.000515 0.000044 0.000385 0.000059 0.000564 0.000066
Nucleus 0.000837 0.000064 0.002342 0.000101 0.000798 0.000050 0.000322 0.000112 0.000813 0.000056 0.000426 0.000110
Peroxisomes 0.025812 0.015900 0.038335 0.037818 0.013348 0.019966 0.021434 0.021707 0.017907 0.018236 0.022308 0.024165
Vacuole 0.006032 0.005624 0.018871 0.018178 0.001197 0.002580 0.012080 0.007859 0.002966 0.003876 0.012431 0.009434
Vacuole Periphery 0.011669 0.006474 0.016467 0.021037 0.002510 0.003015 0.008796 0.006901 0.005860 0.004487 0.009192 0.009058

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.68 -0.58 -3.40 -3.55 -3.25 -2.84 -2.42 -4.79 -3.03 -2.46 -2.24 -2.48 -5.62 -4.60 -2.93
Bud Neck 2.16 -2.97 -0.29 -4.08 2.99 1.48 -4.63 -2.13 -4.00 2.56 2.60 -4.25 -1.45 -4.78 3.06
Bud Site 2.42 -0.27 -1.62 -2.45 -1.52 0.65 -6.02 -5.43 -5.60 -0.13 2.20 -5.62 -5.39 -6.08 -0.07
Cell Periphery 1.80 1.57 0.74 -1.81 -1.38 1.34 2.29 0.74 -0.94 -2.46 2.24 2.61 0.93 -1.89 -2.60
Cytoplasm 0.98 -0.14 0.19 -3.12 0.69 -0.56 -4.58 -5.40 -5.20 0.53 0.78 -2.82 -2.63 -5.61 0.75
Cytoplasmic Foci 0.73 -4.25 -2.26 -2.60 3.31 -5.38 -7.65 -6.58 -1.84 1.70 -3.28 -5.27 -3.86 -1.28 1.81
Eisosomes 1.52 -2.26 -5.17 -5.67 -2.74 -3.24 -3.04 -7.03 -4.59 -5.32 -2.13 -3.01 -8.10 -6.83 -6.18
Endoplasmic Reticulum 4.56 -1.33 -3.00 -3.54 0.84 0.59 -2.21 -3.56 -3.85 -1.38 2.77 -1.97 -4.33 -5.33 -0.84
Endosome 3.98 -2.66 3.30 0.25 3.95 -6.01 -8.06 -4.88 1.08 4.19 -0.93 -3.59 1.42 2.46 4.66
Golgi -0.14 -2.27 -5.51 -5.49 -2.18 -4.92 -6.07 -9.04 -5.95 -3.68 -3.90 -3.87 -9.06 -6.98 -5.34
Lipid Particles 3.33 -1.52 4.26 1.32 2.47 1.27 0.06 2.95 2.59 2.77 3.26 1.42 5.62 3.50 3.43
Mitochondria -4.52 6.33 5.50 7.58 -2.99 6.04 13.19 11.26 6.60 -1.85 1.46 9.85 9.09 8.23 -1.03
Mitotic Spindle 2.38 -3.76 -3.29 -3.60 2.30 -1.77 -9.89 -6.51 -5.21 6.34 0.41 -9.88 -5.80 -5.99 5.34
None 2.33 -1.16 -3.60 -4.85 0.65 1.66 -3.59 -2.42 -8.87 2.15 2.38 -3.41 -3.43 -8.84 1.13
Nuclear Periphery 1.66 -1.74 -2.50 -2.84 1.60 0.35 -4.74 -1.82 -1.87 0.35 1.43 -2.43 -2.33 -2.63 1.38
Nuclear Periphery Foci 1.99 -1.99 -1.65 -3.32 1.91 0.57 -4.92 -1.72 -1.88 0.61 1.94 -2.50 -1.39 -2.50 1.78
Nucleolus 1.62 -1.18 1.33 -1.15 2.41 1.32 -1.25 1.28 -0.42 2.36 2.05 -0.68 2.01 -0.39 2.60
Nucleus 1.42 -1.22 1.36 -1.36 2.25 1.56 0.96 1.29 -1.35 1.01 2.09 1.04 1.78 -1.39 1.81
Peroxisomes 2.84 -1.36 -1.31 -2.49 0.04 -2.61 -3.13 -2.88 -0.55 -0.07 -0.16 -1.77 -2.18 -2.11 -0.57
Vacuole 0.47 -3.32 -2.89 -2.98 0.12 -3.71 -8.21 -5.66 -4.45 2.14 -2.18 -7.38 -5.46 -4.63 1.53
Vacuole Periphery 4.08 -1.57 -1.81 -2.85 -0.79 -1.12 -5.94 -4.96 -4.41 1.46 2.44 -3.11 -2.79 -4.17 0.10
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrpl27

Mrpl27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available