Standard name
Human Ortholog
Description Vacuolar aminopeptidase Y; processed to mature form by Prb1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.98 0.97 0.93 0.91 0.89 0.93 0.9 0.89 0.85 0.89 0.98 0.96 0.98 0.89 0.89 0.89 0.53 0.82 0.62 0.65 0.67 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0 0 0 0 0 0 0 0.16 0.05 0 0 0 0 0 0 0.13 0 0.11 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.15 0.13 0.23 0.1 0.13 0.16 0.11 0.11 0 0 0 0.13 0.11 0.11 0.21 0.1 0.18 0.21 0.2 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 2
Bud 1 0 0 0 0 0 2 0 0 4 1 0 2 0 1 0 2 0 0 0 0 0 0 3
Bud Neck 0 2 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 0 0 0 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 1 2 1 0 0 2 1 1 0 1 0 1 0 0 1 4 0 0 0 0 0 0
Cytoplasm 174 202 124 242 290 329 357 415 208 296 188 294 134 241 268 98 171 120 101 180 87 46 83 149
Endoplasmic Reticulum 1 1 0 2 0 0 0 1 0 0 0 2 0 1 0 5 18 9 4 0 2 0 1 0
Endosome 0 0 0 0 0 0 2 3 0 1 0 2 0 2 2 2 0 2 6 2 1 0 0 2
Golgi 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Mitochondria 19 3 0 0 0 3 2 5 5 5 35 17 0 2 0 3 4 1 25 8 15 3 4 4
Nucleus 0 0 1 1 0 8 11 10 10 2 5 4 2 0 1 0 2 1 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 1 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 0 0 2 0 1 0 1 1 1 0 0 0 0 1 0 0 0 0 1
Vac/Vac Membrane 5 3 1 2 46 48 93 46 30 54 24 36 0 2 0 14 21 15 41 21 25 15 24 41
Unique Cell Count 186 208 126 249 313 362 403 448 232 331 222 330 137 250 273 110 193 135 191 221 141 71 125 217
Labelled Cell Count 201 213 129 254 339 389 467 485 254 364 253 358 140 251 274 122 219 153 191 221 141 71 125 217


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.9 5.3 5.0 6.0 4.8 5.2 5.4 4.6 4.7 4.2 4.5 4.7 5.3 5.3 5.7 5.7 5.3 4.4 5.2 5.3
Std Deviation (1e-4) 0.5 0.5 2.1 1.7 2.0 1.2 1.2 1.8 1.3 1.1 1.2 1.4 1.1 1.5 2.1 1.6 1.6 1.1 1.0 2.0 1.7
Intensity Change (Log2) – – – -0.1 0.17 -0.13 -0.04 0.03 -0.22 -0.18 -0.35 -0.25 -0.17 -0.0 -0.02 0.1 0.1 0.01 -0.29 -0.03 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.7 3.5 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8527 -0.5142 -0.6435 -0.1294 -0.5451 -0.4966 -1.8007 -0.9791 -0.9215 -1.3667 -0.9183 -1.2272 -1.1359 -1.0435 -1.2442 -0.3614 -0.9353 -1.2643
Actin 0.0175 0.002 0.0051 0.0469 0.0132 0.0067 0.0164 0.0001 0.0008 0.0001 0.0059 0.0021 0.0208 0.0001 0.0015 0.0063 0.0091 0.0084
Bud 0.0016 0.0062 0.002 0.0025 0.0019 0.0013 0.0018 0.0076 0.0064 0.0002 0.011 0.0001 0.0018 0.0009 0.0017 0.0014 0.0088 0.0003
Bud Neck 0.0051 0.0005 0.0047 0.0016 0.007 0.0066 0.0004 0.0005 0.0012 0.0001 0.0006 0.0004 0.0078 0.0007 0.0041 0.0044 0.003 0.0014
Bud Periphery 0.0051 0.0088 0.0051 0.0051 0.0055 0.0026 0.0016 0.0014 0.0144 0.0003 0.044 0.0003 0.004 0.0012 0.0052 0.0021 0.0145 0.0005
Bud Site 0.004 0.0107 0.0068 0.0037 0.0085 0.0014 0.0009 0.0137 0.01 0.0003 0.0026 0.0005 0.008 0.0138 0.0046 0.0079 0.0136 0.0005
Cell Periphery 0.0005 0.0004 0.0003 0.0002 0.0004 0.0002 0.0002 0.0006 0.0011 0.0001 0.0006 0.0002 0.0006 0.0005 0.0007 0.0011 0.0008 0.0002
Cytoplasm 0.1136 0.1056 0.0795 0.1503 0.1261 0.1546 0.0357 0.0474 0.0185 0.0483 0.0156 0.1259 0.1177 0.1696 0.1314 0.1068 0.0872 0.2518
Cytoplasmic Foci 0.0474 0.0224 0.0401 0.0795 0.0667 0.0424 0.0193 0.012 0.031 0.0344 0.0592 0.0223 0.054 0.0229 0.0236 0.0934 0.0449 0.0528
Eisosomes 0.0003 0.0002 0.0004 0.0002 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0004 0.0002 0.0013 0.0003 0.0004 0.0001
Endoplasmic Reticulum 0.0029 0.0013 0.0021 0.0021 0.0014 0.0018 0.0015 0.001 0.0012 0.0005 0.0007 0.0017 0.0048 0.0028 0.0018 0.0019 0.0064 0.0021
Endosome 0.0547 0.0194 0.0404 0.0825 0.0665 0.0398 0.0155 0.012 0.0544 0.0363 0.0563 0.01 0.0437 0.0332 0.0344 0.0825 0.0963 0.0381
Golgi 0.0043 0.0007 0.0019 0.009 0.0075 0.0019 0.002 0.0003 0.004 0.0006 0.0118 0.0015 0.0084 0.0005 0.0012 0.01 0.0138 0.0029
Lipid Particles 0.0094 0.0011 0.0047 0.0044 0.0193 0.0095 0.0054 0.0028 0.0127 0.0018 0.0046 0.0063 0.028 0.003 0.0084 0.0182 0.0273 0.0144
Mitochondria 0.0093 0.0017 0.0034 0.0089 0.0129 0.0038 0.0027 0.0008 0.0103 0.003 0.1669 0.0044 0.0148 0.001 0.0028 0.0222 0.0113 0.0036
None 0.6702 0.7798 0.7515 0.5097 0.5125 0.6681 0.8745 0.8698 0.7366 0.8503 0.5828 0.802 0.5765 0.6884 0.7026 0.5477 0.5199 0.5693
Nuclear Periphery 0.0031 0.001 0.005 0.0018 0.0022 0.0021 0.0013 0.0005 0.002 0.0006 0.0009 0.0022 0.0099 0.0011 0.0016 0.0021 0.0095 0.0012
Nucleolus 0.0024 0.0022 0.0018 0.0024 0.0028 0.0011 0.0011 0.0032 0.0033 0.0004 0.0008 0.0006 0.0027 0.001 0.0032 0.0051 0.0027 0.0009
Nucleus 0.0046 0.0048 0.0097 0.0055 0.0063 0.0041 0.0015 0.0031 0.0043 0.0011 0.0009 0.0054 0.0052 0.0026 0.0047 0.0051 0.0087 0.0023
Peroxisomes 0.0053 0.001 0.0059 0.0079 0.0203 0.0068 0.0011 0.0002 0.0047 0.0007 0.004 0.0016 0.0345 0.0007 0.0018 0.0172 0.0098 0.0081
Punctate Nuclear 0.0041 0.0071 0.0091 0.0024 0.0043 0.0042 0.0011 0.0017 0.0141 0.0007 0.0018 0.0013 0.0074 0.0008 0.0065 0.0044 0.0221 0.0047
Vacuole 0.0317 0.0215 0.0183 0.0664 0.1054 0.0376 0.0146 0.0203 0.0527 0.0167 0.0205 0.0104 0.0447 0.0521 0.0523 0.0543 0.0788 0.0326
Vacuole Periphery 0.003 0.0015 0.0021 0.0069 0.0091 0.0034 0.001 0.0008 0.016 0.0033 0.0084 0.0008 0.0042 0.0029 0.0046 0.0053 0.0111 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 496.3269 479.1319 557.7933 587.5701 526.5078 484.4366 563.2347 584.7108 566.8887 529.8523
Translational Efficiency 1.4711 1.4262 1.3267 1.1873 1.1774 1.6165 1.3513 1.201 1.3094 1.3247

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
960 1102 1259 393 848 1982 79 785 1808 3084 1338 1178

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 566.37 684.54 867.66 818.69 711.36 679.82 929.83 778.94 634.37 681.51 871.33 792.20
Standard Deviation 60.56 86.23 100.76 118.94 81.12 82.45 110.59 110.31 101.34 83.85 102.42 114.80
Intensity Change Log 2 — 0.273390 0.615385 0.531572 — -0.065427 0.386387 0.130932 — 0.094641 0.492401 0.322350

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000158 0.000234 0.000414 0.000200 0.000302 0.000128 0.000313 0.001155 0.000226 0.000166 0.000408 0.000837
Bud Neck 0.042017 0.022615 0.010066 0.004403 0.008718 0.039354 0.007064 0.012378 0.026399 0.033373 0.009889 0.009718
Bud Site 0.005336 0.009840 0.011707 0.005855 0.002966 0.013567 0.008583 0.038031 0.004225 0.012235 0.011522 0.027297
Cell Periphery 0.000201 0.000323 0.000145 0.000186 0.000163 0.000102 0.000227 0.000226 0.000184 0.000181 0.000150 0.000213
Cytoplasm 0.405532* 0.443131* 0.402844* 0.455235* 0.528022* 0.376260* 0.245341* 0.484662* 0.462983* 0.400155* 0.393544* 0.474845*
Cytoplasmic Foci 0.278069* 0.238970* 0.021867 0.035750 0.197657* 0.236388* 0.026160 0.030612 0.240353* 0.237311* 0.022121 0.032326
Eisosomes 0.000423 0.000109 0.000046 0.000068 0.000144 0.000109 0.000111 0.000062 0.000293 0.000109 0.000050 0.000064
Endoplasmic Reticulum 0.000698 0.000621 0.005291 0.001525 0.000665 0.000597 0.005216 0.002158 0.000682 0.000606 0.005287 0.001947
Endosome 0.032196 0.027645 0.008914 0.009607 0.010764 0.040034 0.007116 0.008955 0.022144 0.035607 0.008807 0.009173
Golgi 0.003157 0.003301 0.000275 0.016293 0.001255 0.004526 0.000090 0.002799 0.002265 0.004089 0.000264 0.007301
Lipid Particles 0.017637 0.006963 0.002801 0.002064 0.006341 0.007753 0.003865 0.001498 0.012339 0.007470 0.002864 0.001687
Mitochondria 0.005764 0.009042 0.000768 0.003039 0.002519 0.014945 0.000327 0.006660 0.004242 0.012836 0.000742 0.005452
Mitotic Spindle 0.000288 0.002682 0.012678 0.021820 0.006641 0.009731 0.005686 0.013056 0.003268 0.007212 0.012266 0.015980
None 0.004706 0.004169 0.003388 0.009706 0.003214 0.002408 0.003137 0.004967 0.004006 0.003037 0.003373 0.006548
Nuclear Periphery 0.000435 0.000589 0.003157 0.001352 0.000556 0.000556 0.002005 0.001301 0.000492 0.000568 0.003089 0.001318
Nuclear Periphery Foci 0.000372 0.000438 0.005475 0.001324 0.000395 0.000339 0.003089 0.001745 0.000383 0.000375 0.005334 0.001605
Nucleolus 0.001685 0.002345 0.001451 0.000891 0.000886 0.002262 0.000570 0.000655 0.001310 0.002292 0.001399 0.000734
Nucleus 0.109243 0.123509 0.425732* 0.239179* 0.176761* 0.106447 0.460103* 0.237070* 0.140911* 0.112543 0.427762* 0.237774*
Peroxisomes 0.009446 0.014976 0.001698 0.000371 0.005317 0.012094 0.000352 0.002120 0.007510 0.013123 0.001619 0.001536
Vacuole 0.081383 0.085564 0.080934 0.187174 0.045933 0.128607 0.220197* 0.148067 0.064756 0.113226 0.089156 0.161114
Vacuole Periphery 0.001250 0.002935 0.000350 0.003956 0.000779 0.003793 0.000447 0.001821 0.001029 0.003486 0.000355 0.002533

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.29 -6.18 -0.86 0.74 6.31 1.05 -0.36 -1.51 -3.33 -1.72 0.71 -3.20 -2.06 -3.12 0.20
Bud Neck 5.35 8.68 11.84 9.29 4.64 -13.84 0.18 0.07 12.33 -0.14 -3.15 6.44 9.31 14.77 2.52
Bud Site -3.15 -3.24 0.09 2.93 3.08 -7.92 -1.55 -5.64 -2.15 -2.55 -7.90 -4.16 -5.28 -1.47 -1.85
Cell Periphery -0.78 3.18 2.90 1.21 0.79 4.15 -1.29 0.41 -2.94 1.52 0.17 2.25 2.04 0.48 0.43
Cytoplasm -2.95 4.90 2.61 4.61 -0.58 13.82 13.85 9.85 -1.25 -7.79 7.91 12.62 7.66 1.79 -3.45
Cytoplasmic Foci 4.45 37.86 35.49 31.55 -1.35 -4.76 22.91 26.45 41.33 -0.93 0.66 44.51 43.95 52.02 -1.20
Eisosomes 10.88 12.83 11.77 3.98 -5.51 3.78 1.32 7.68 8.06 3.95 11.31 14.30 13.37 8.90 -4.30
Endoplasmic Reticulum 0.55 -18.98 -7.27 -8.20 12.55 0.41 -6.24 -9.45 -11.50 3.56 0.71 -19.97 -12.12 -13.87 12.02
Endosome 1.65 9.57 10.86 10.38 2.55 -14.14 2.61 4.25 18.45 1.27 -7.25 8.23 11.75 21.11 3.56
Golgi -0.09 4.93 -0.96 -0.93 -1.86 -6.33 3.78 0.28 5.26 -2.03 -3.64 5.80 -0.48 1.02 -2.28
Lipid Particles 6.29 8.84 9.64 9.34 4.67 -1.53 2.69 6.19 11.37 3.77 4.85 9.50 11.21 14.51 6.36
Mitochondria -1.97 5.49 3.02 4.39 -0.71 -7.35 2.05 -0.24 7.30 -2.51 -7.21 4.91 1.76 8.45 -2.11
Mitotic Spindle -3.16 -6.58 -2.63 -1.91 1.58 -1.07 0.39 -0.63 0.55 -0.83 -2.65 -4.99 -2.91 -0.96 2.61
None 0.48 1.55 -0.90 -1.63 -2.91 1.51 0.30 -1.71 -3.28 -1.55 1.50 1.50 -1.13 -3.38 -3.64
Nuclear Periphery -1.96 -16.91 -9.73 -8.32 9.17 -0.05 -8.68 -8.43 -9.98 1.13 -1.08 -16.88 -12.41 -12.53 8.57
Nuclear Periphery Foci -1.52 -8.18 -5.05 -4.56 6.49 0.57 -4.56 -6.29 -6.11 2.37 0.05 -8.46 -7.73 -7.32 6.10
Nucleolus -2.12 -1.43 5.03 5.58 4.65 -6.18 2.17 2.41 7.50 -0.12 -5.06 -2.51 5.54 9.33 4.90
Nucleus -2.40 -35.48 -11.33 -9.89 12.51 10.16 -6.75 -7.63 -16.32 4.33 6.28 -32.87 -14.46 -19.00 16.05
Peroxisomes -3.25 7.93 10.54 9.69 4.06 -4.86 4.66 3.82 11.94 -2.21 -5.46 7.34 9.33 15.28 2.35
Vacuole -1.55 -6.28 -14.91 -14.40 -12.31 -18.42 -7.70 -21.04 -12.52 1.32 -13.82 -11.78 -24.70 -18.69 -15.35
Vacuole Periphery -3.93 7.56 -0.32 0.98 -1.10 -7.63 2.03 -0.02 6.14 -1.15 -8.44 6.67 -0.08 4.53 -1.60
Standard name
Human Ortholog
Description Vacuolar aminopeptidase Y; processed to mature form by Prb1p
Localization
Cell Percentages cytoplasm (28%), vacuole (2%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ape3

Ape3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ape3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available