ORF | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 15 | 28 | 32 | 16 | 8 | 7 | – | 4 | 2 | 4 | 4 | 4 | 10 | 6 | 12 | 7 | 1 | 1 | 10 | 3 | 13 | 19 |
Bud | 0 | 1 | 6 | 1 | 1 | 6 | 6 | 15 | – | 13 | 22 | 7 | 0 | 0 | 1 | 2 | 4 | 1 | 1 | 0 | 2 | 4 | 9 | 10 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 18 | 11 | 29 | 25 | 16 | 7 | – | 8 | 7 | 9 | 29 | 29 | 34 | 0 | 13 | 10 | 0 | 0 | 4 | 1 | 8 | 9 |
Cytoplasm | 13 | 19 | 71 | 40 | 53 | 98 | 225 | 252 | – | 263 | 205 | 242 | 44 | 44 | 96 | 32 | 83 | 36 | 2 | 5 | 6 | 3 | 15 | 15 |
Endoplasmic Reticulum | 35 | 72 | 258 | 212 | 431 | 271 | 145 | 179 | – | 72 | 28 | 47 | 208 | 208 | 149 | 2 | 7 | 2 | 92 | 101 | 420 | 209 | 332 | 424 |
Endosome | 0 | 0 | 6 | 10 | 4 | 5 | 13 | 6 | – | 2 | 1 | 3 | 0 | 0 | 0 | 1 | 6 | 2 | 0 | 0 | 1 | 1 | 3 | 2 |
Golgi | 0 | 0 | 1 | 8 | 5 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 3 | 4 |
Mitochondria | 1 | 5 | 35 | 26 | 14 | 71 | 96 | 145 | – | 235 | 218 | 220 | 0 | 0 | 0 | 6 | 10 | 10 | 6 | 3 | 16 | 7 | 15 | 16 |
Nucleus | 0 | 1 | 0 | 1 | 0 | 0 | 5 | 11 | – | 2 | 3 | 6 | 2 | 2 | 3 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 2 | 0 | 4 | 6 | 7 | 14 | 10 | 12 | – | 7 | 4 | 12 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 2 | 1 | 3 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 2 | 7 | 67 | 40 | 91 | 84 | 212 | 93 | – | 66 | 43 | 62 | 9 | 8 | 5 | 6 | 39 | 15 | 2 | 4 | 7 | 6 | 18 | 20 |
Unique Cell Count | 52 | 98 | 408 | 303 | 546 | 491 | 623 | 631 | 551 | 439 | 517 | 259 | 260 | 263 | 51 | 163 | 78 | 118 | 121 | 479 | 244 | 429 | 531 | |
Labelled Cell Count | 54 | 105 | 482 | 383 | 667 | 591 | 736 | 729 | 675 | 534 | 615 | 296 | 295 | 299 | 58 | 187 | 89 | 118 | 121 | 479 | 244 | 429 | 531 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 8.9 | 7.1 | 9.2 | 10.3 | 7.6 | 6.1 | 5.3 | – | 4.2 | 4.3 | 4.3 | 9.3 | 9.3 | 8.2 | 6.4 | 8.3 | 9.3 | 11.1 | 11.8 | 12.6 |
Std Deviation (1e-4) | 1.7 | 2.1 | 1.4 | 1.9 | 1.6 | 1.4 | 1.3 | 1.0 | – | 0.7 | 0.8 | 0.7 | 1.7 | 1.7 | 1.3 | 2.0 | 2.7 | 2.5 | 2.6 | 3.4 | 3.2 |
Intensity Change (Log2) | – | – | – | 0.37 | 0.53 | 0.09 | -0.21 | -0.43 | – | -0.74 | -0.71 | -0.72 | 0.39 | 0.39 | 0.21 | -0.15 | 0.23 | 0.39 | 0.64 | 0.73 | 0.82 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 3.1 | 1.5 | -0.3 | -2.5 | -2.8 | – | 0 | 0 | 0 | -1.6 | -1.6 | 0.1 | 0 | 1.9 | 0 |
Bud | 0 | 0 | -0.3 | 0 | 1.0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.5 | 0.6 | 0.5 | -1.6 | -3.4 | – | 0 | 0 | 0 | 3.3 | 3.3 | 4.0 | 0 | 1.7 | 0 |
Cytoplasm | -1.5 | -3.5 | 1.0 | 6.5 | 7.7 | – | 9.7 | 9.1 | 9.4 | -0.1 | -0.2 | 5.6 | 7.3 | 8.2 | 5.6 |
Endoplasmic Reticulum | 1.9 | 5.4 | -2.4 | -12.9 | -11.1 | – | 0 | 0 | 0 | 4.7 | 4.6 | -1.7 | -8.1 | -12.8 | -9.8 |
Endosome | 1.6 | 0 | 0 | 0.7 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -4.2 | 2.7 | 3.2 | 6.0 | – | 0 | 0 | 0 | -4.8 | -4.9 | -4.9 | 0 | -1.0 | 1.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2.0 | 0.8 | 1.2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.2 | 0.1 | 0.3 | 6.2 | -0.7 | – | -2.0 | -2.9 | -1.9 | -5.1 | -5.3 | -5.9 | -0.9 | 2.1 | 0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4896 | 3.2391 | 2.8764 | 2.6998 | 2.267 | 3.306 | 1.6576 | 3.9921 | 2.3916 | 2.6108 | 2.0898 | 2.6017 | 1.91 | 4.7819 | 2.9191 | 3.6542 | 2.1402 | 3.006 |
Actin | 0.0425 | 0.002 | 0.014 | 0.0394 | 0.0009 | 0.002 | 0.0071 | 0.0006 | 0.0074 | 0.0031 | 0.0023 | 0.0045 | 0.0092 | 0.0007 | 0.0016 | 0.0024 | 0.0005 | 0.0015 |
Bud | 0.0003 | 0.002 | 0.0014 | 0.0005 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Bud Neck | 0.0029 | 0.0004 | 0.0007 | 0.0372 | 0.0035 | 0.002 | 0.0007 | 0.0005 | 0.0004 | 0.0011 | 0.0005 | 0.0007 | 0.0012 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0026 |
Bud Periphery | 0.0005 | 0.0008 | 0.001 | 0.0007 | 0.0008 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0032 | 0.0052 | 0.003 | 0.0176 | 0.0177 | 0.0001 | 0.0057 | 0.0053 | 0.0013 | 0.0004 | 0.0005 | 0.0001 | 0.0032 | 0.0023 | 0.0011 | 0.0001 | 0.0002 | 0.0001 |
Cell Periphery | 0.0012 | 0.0094 | 0.0149 | 0.0008 | 0.0012 | 0.0005 | 0.0013 | 0.0032 | 0.0017 | 0.0011 | 0.0007 | 0.0008 | 0.0013 | 0.0015 | 0.0011 | 0.0004 | 0.0006 | 0.0021 |
Cytoplasm | 0.2388 | 0.3751 | 0.247 | 0.2229 | 0.2614 | 0.4068 | 0.1568 | 0.2049 | 0.1884 | 0.1594 | 0.1537 | 0.1791 | 0.1492 | 0.1463 | 0.1746 | 0.1569 | 0.2078 | 0.1794 |
Cytoplasmic Foci | 0.0338 | 0.0171 | 0.0166 | 0.0074 | 0.0082 | 0.0128 | 0.0259 | 0.0135 | 0.0128 | 0.0074 | 0.0161 | 0.0084 | 0.0139 | 0.0056 | 0.0063 | 0.0039 | 0.0044 | 0.0094 |
Eisosomes | 0.0009 | 0.0002 | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.4345 | 0.436 | 0.4681 | 0.4089 | 0.4419 | 0.4256 | 0.5829 | 0.6443 | 0.6385 | 0.6404 | 0.6682 | 0.6545 | 0.6622 | 0.757 | 0.7026 | 0.7167 | 0.6325 | 0.7241 |
Endosome | 0.0353 | 0.0134 | 0.0295 | 0.0235 | 0.0137 | 0.0139 | 0.0388 | 0.0228 | 0.0193 | 0.04 | 0.0314 | 0.0206 | 0.0298 | 0.0097 | 0.0122 | 0.0353 | 0.0053 | 0.0161 |
Golgi | 0.0079 | 0.001 | 0.0101 | 0.0132 | 0.0011 | 0.0011 | 0.0101 | 0.0011 | 0.0052 | 0.0025 | 0.0024 | 0.0024 | 0.0052 | 0.0008 | 0.0021 | 0.0031 | 0.0003 | 0.0005 |
Lipid Particles | 0.0144 | 0.0053 | 0.0109 | 0.0117 | 0.0044 | 0.0064 | 0.0295 | 0.0143 | 0.0166 | 0.0036 | 0.0073 | 0.0072 | 0.0182 | 0.0131 | 0.01 | 0.0147 | 0.0024 | 0.0035 |
Mitochondria | 0.0018 | 0.0006 | 0.0021 | 0.0018 | 0.0006 | 0.0006 | 0.0018 | 0.0003 | 0.0037 | 0.0041 | 0.0023 | 0.0039 | 0.0016 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0001 |
None | 0.1232 | 0.0925 | 0.1133 | 0.1563 | 0.2042 | 0.1007 | 0.0963 | 0.063 | 0.0659 | 0.1175 | 0.0504 | 0.0943 | 0.062 | 0.0392 | 0.0688 | 0.0038 | 0.1263 | 0.0349 |
Nuclear Periphery | 0.0209 | 0.0174 | 0.0245 | 0.0398 | 0.0129 | 0.016 | 0.0178 | 0.0127 | 0.0199 | 0.0086 | 0.0281 | 0.0136 | 0.0274 | 0.0134 | 0.0094 | 0.0475 | 0.0063 | 0.0123 |
Nucleolus | 0.0005 | 0.0004 | 0.0011 | 0.0005 | 0.0033 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0093 | 0.0064 | 0.0157 | 0.0073 | 0.0102 | 0.0058 | 0.0021 | 0.0028 | 0.0022 | 0.0022 | 0.0237 | 0.002 | 0.0033 | 0.0023 | 0.0014 | 0.0041 | 0.0088 | 0.0025 |
Peroxisomes | 0.0098 | 0.0017 | 0.0082 | 0.0015 | 0.0004 | 0.0003 | 0.0094 | 0.0001 | 0.0072 | 0.0001 | 0.0003 | 0.0003 | 0.0026 | 0.0001 | 0.0022 | 0.0001 | 0 | 0.0001 |
Punctate Nuclear | 0.0075 | 0.0038 | 0.0054 | 0.0023 | 0.003 | 0.0011 | 0.0046 | 0.0018 | 0.0009 | 0.001 | 0.003 | 0.0012 | 0.002 | 0.001 | 0.0007 | 0.0016 | 0.0007 | 0.0007 |
Vacuole | 0.0096 | 0.0083 | 0.0102 | 0.0045 | 0.0093 | 0.0037 | 0.0076 | 0.0076 | 0.007 | 0.0055 | 0.0065 | 0.0049 | 0.006 | 0.0062 | 0.0044 | 0.0066 | 0.003 | 0.0094 |
Vacuole Periphery | 0.0013 | 0.0007 | 0.0022 | 0.0015 | 0.0005 | 0.0004 | 0.0008 | 0.0005 | 0.0008 | 0.001 | 0.0015 | 0.0009 | 0.0009 | 0.0004 | 0.0004 | 0.0016 | 0.0002 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 64.0215 | 45.3241 | 66.1248 | 70.2951 | 44.5674 | 81.593 | 56.5073 | 72.6075 | 85.9261 | 63.9622 |
Translational Efficiency | 0.6353 | 0.6668 | 0.6359 | 0.8273 | 0.7341 | 0.6793 | 0.7147 | 0.6175 | 0.7163 | 0.6039 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
85 | 601 | 430 | 1032 | 1545 | 1264 | 655 | 1503 | 1630 | 1865 | 1085 | 2535 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1165.68 | 1057.04 | 1262.03 | 1688.89 | 929.00 | 887.98 | 1496.90 | 1534.95 | 941.34 | 942.46 | 1403.82 | 1597.62 |
Standard Deviation | 163.47 | 184.72 | 172.75 | 341.92 | 146.68 | 126.54 | 241.76 | 301.89 | 156.70 | 167.60 | 245.58 | 327.64 |
Intensity Change Log 2 | — | -0.141142 | 0.114574 | 0.534904 | — | -0.065151 | 0.688227 | 0.724441 | — | -0.106945 | 0.397379 | 0.622050 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.007321 | 0.001039 | 0.001530 | 0.001623 | 0.000513 | 0.001121 | 0.001151 | 0.002474 | 0.000868 | 0.001095 | 0.001301 | 0.002127 |
Bud Neck | 0.010437 | 0.001456 | 0.002548 | 0.002123 | 0.000454 | 0.001207 | 0.001299 | 0.003055 | 0.000975 | 0.001288 | 0.001794 | 0.002675 |
Bud Site | 0.028931 | 0.006424 | 0.007672 | 0.012447 | 0.003468 | 0.003836 | 0.004810 | 0.017093 | 0.004796 | 0.004670 | 0.005944 | 0.015202 |
Cell Periphery | 0.003060 | 0.002522 | 0.000841 | 0.026874 | 0.002866 | 0.000796 | 0.003100 | 0.018791 | 0.002877 | 0.001352 | 0.002205 | 0.022082 |
Cytoplasm | 0.087352 | 0.075967 | 0.038333 | 0.012844 | 0.054414 | 0.118570* | 0.005619 | 0.024446 | 0.056132 | 0.104841* | 0.018584 | 0.019723 |
Cytoplasmic Foci | 0.021517 | 0.014930 | 0.006994 | 0.001925 | 0.008928 | 0.036164 | 0.000392 | 0.003684 | 0.009584 | 0.029321 | 0.003009 | 0.002968 |
Eisosomes | 0.001426 | 0.000424 | 0.000049 | 0.000294 | 0.000336 | 0.000402 | 0.000079 | 0.000189 | 0.000393 | 0.000409 | 0.000067 | 0.000231 |
Endoplasmic Reticulum | 0.576518* | 0.833300* | 0.785700* | 0.875303* | 0.886076* | 0.714587* | 0.962257* | 0.848642* | 0.869933* | 0.752842* | 0.892285* | 0.859496* |
Endosome | 0.014579 | 0.005890 | 0.013182 | 0.007822 | 0.003343 | 0.009255 | 0.004834 | 0.012059 | 0.003929 | 0.008171 | 0.008143 | 0.010334 |
Golgi | 0.001563 | 0.006127 | 0.000922 | 0.005765 | 0.006354 | 0.013567 | 0.001167 | 0.006193 | 0.006104 | 0.011169 | 0.001070 | 0.006019 |
Lipid Particles | 0.034607 | 0.014475 | 0.009852 | 0.027755 | 0.010991 | 0.042303 | 0.004848 | 0.031337 | 0.012223 | 0.033335 | 0.006831 | 0.029879 |
Mitochondria | 0.000198 | 0.001793 | 0.000180 | 0.000642 | 0.004646 | 0.007797 | 0.000110 | 0.000740 | 0.004414 | 0.005862 | 0.000138 | 0.000700 |
Mitotic Spindle | 0.001276 | 0.000469 | 0.001413 | 0.001247 | 0.000355 | 0.000354 | 0.000082 | 0.001440 | 0.000403 | 0.000391 | 0.000609 | 0.001361 |
None | 0.000076 | 0.003432 | 0.000134 | 0.000423 | 0.005041 | 0.003164 | 0.000287 | 0.001443 | 0.004782 | 0.003251 | 0.000227 | 0.001027 |
Nuclear Periphery | 0.006249 | 0.001569 | 0.012549 | 0.001140 | 0.000576 | 0.004492 | 0.000487 | 0.000738 | 0.000872 | 0.003550 | 0.005267 | 0.000902 |
Nuclear Periphery Foci | 0.033013 | 0.015116 | 0.082192 | 0.006364 | 0.003224 | 0.009509 | 0.005870 | 0.011362 | 0.004777 | 0.011315 | 0.036117 | 0.009328 |
Nucleolus | 0.000199 | 0.000095 | 0.000039 | 0.000013 | 0.000208 | 0.000235 | 0.000005 | 0.000015 | 0.000208 | 0.000190 | 0.000018 | 0.000014 |
Nucleus | 0.030952 | 0.005247 | 0.007196 | 0.000987 | 0.002488 | 0.013133 | 0.000173 | 0.000792 | 0.003973 | 0.010592 | 0.002956 | 0.000871 |
Peroxisomes | 0.001177 | 0.000916 | 0.000240 | 0.000148 | 0.002688 | 0.002556 | 0.000051 | 0.000677 | 0.002610 | 0.002027 | 0.000126 | 0.000462 |
Vacuole | 0.139124 | 0.008249 | 0.028090 | 0.012593 | 0.002233 | 0.014613 | 0.003176 | 0.013508 | 0.009372 | 0.012562 | 0.013050 | 0.013136 |
Vacuole Periphery | 0.000423 | 0.000563 | 0.000343 | 0.001667 | 0.000796 | 0.002337 | 0.000203 | 0.001321 | 0.000777 | 0.001765 | 0.000258 | 0.001462 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.85 | 1.61 | 1.64 | -1.33 | 0.06 | -1.43 | -0.87 | -4.84 | -2.44 | -1.67 | -0.64 | -0.97 | -3.76 | -2.66 | -1.37 |
Bud Neck | 2.39 | 2.04 | 2.16 | -1.43 | 0.53 | -3.24 | -1.83 | -6.84 | -4.45 | -3.16 | -1.01 | -2.09 | -5.37 | -4.45 | -2.14 |
Bud Site | 2.27 | 2.11 | 1.62 | -2.42 | -1.70 | -0.37 | -0.82 | -6.63 | -6.17 | -4.95 | 0.22 | -0.74 | -6.50 | -6.49 | -4.88 |
Cell Periphery | 0.30 | 1.98 | -9.58 | -10.04 | -11.35 | 6.59 | -0.43 | -9.59 | -11.01 | -9.24 | 3.80 | 1.59 | -13.98 | -15.11 | -14.45 |
Cytoplasm | 0.82 | 2.83 | 4.30 | 9.86 | 4.75 | -11.50 | 17.02 | 8.39 | 17.46 | -8.24 | -10.29 | 10.93 | 11.34 | 20.29 | -0.68 |
Cytoplasmic Foci | 1.51 | 2.87 | 3.87 | 5.29 | 3.88 | -10.48 | 8.26 | 4.42 | 13.21 | -6.20 | -9.44 | 5.64 | 6.16 | 14.21 | 0.22 |
Eisosomes | 1.14 | 1.61 | 1.31 | 0.94 | -3.31 | -0.78 | 4.35 | 2.43 | 3.03 | -3.32 | -0.23 | 4.69 | 2.09 | 2.65 | -4.57 |
Endoplasmic Reticulum | -6.52 | -5.25 | -7.41 | -2.69 | -5.98 | 15.81 | -10.99 | 5.02 | -10.97 | 14.52 | 12.18 | -2.40 | 1.84 | -11.16 | 4.25 |
Endosome | 3.06 | 0.56 | 2.44 | -1.79 | 3.84 | -6.82 | -2.52 | -12.16 | -2.94 | -8.50 | -5.93 | -6.33 | -11.44 | -3.03 | -2.92 |
Golgi | -5.18 | 1.25 | -3.57 | 0.26 | -4.24 | -4.79 | 6.70 | 0.09 | 4.51 | -5.19 | -4.38 | 7.16 | 0.05 | 4.33 | -6.69 |
Lipid Particles | 1.80 | 2.17 | 0.56 | -3.80 | -5.29 | -11.56 | 4.09 | -7.66 | 2.79 | -9.81 | -9.78 | 3.52 | -8.14 | 1.04 | -10.97 |
Mitochondria | -2.63 | 0.50 | -2.35 | 1.80 | -2.54 | -1.77 | 4.71 | 3.99 | 4.70 | -3.91 | -1.05 | 4.68 | 4.01 | 4.96 | -4.65 |
Mitotic Spindle | 1.26 | -0.12 | 0.06 | -1.06 | 0.17 | -0.01 | 1.28 | -1.94 | -2.00 | -2.46 | 0.08 | -0.77 | -2.09 | -2.21 | -1.48 |
None | -2.44 | -0.85 | -3.19 | 2.18 | -2.73 | 1.60 | 5.31 | 3.50 | 1.77 | -1.96 | 1.42 | 5.46 | 4.23 | 2.87 | -2.28 |
Nuclear Periphery | 2.01 | -2.60 | 2.20 | 1.06 | 8.45 | -5.84 | 0.57 | -1.49 | 5.59 | -2.40 | -5.52 | -7.54 | -0.55 | 5.59 | 7.63 |
Nuclear Periphery Foci | 2.41 | -4.84 | 3.50 | 3.37 | 11.50 | -6.38 | -1.60 | -7.23 | -1.70 | -3.04 | -6.02 | -10.41 | -5.28 | 1.02 | 8.55 |
Nucleolus | 1.44 | 1.98 | 2.29 | 3.02 | 2.45 | -0.14 | 1.33 | 1.25 | 3.75 | -5.04 | 0.15 | 1.31 | 1.34 | 4.32 | 0.98 |
Nucleus | 2.84 | 2.63 | 3.19 | 5.72 | 4.11 | -9.10 | 7.30 | 4.63 | 10.76 | -4.16 | -5.79 | 1.38 | 4.68 | 11.84 | 3.32 |
Peroxisomes | 0.95 | 3.20 | 3.71 | 3.16 | 1.44 | 0.28 | 7.89 | 4.53 | 4.56 | -2.11 | 1.52 | 7.77 | 5.95 | 5.71 | -1.80 |
Vacuole | 5.93 | 5.03 | 5.70 | -2.76 | 4.45 | -7.71 | -1.64 | -9.80 | 0.03 | -8.38 | -1.00 | -1.64 | -2.04 | -1.20 | -0.15 |
Vacuole Periphery | -0.85 | 0.57 | -3.25 | -2.77 | -3.50 | -3.23 | 4.07 | -2.31 | 2.03 | -5.88 | -2.91 | 3.78 | -3.05 | 0.76 | -6.29 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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