Standard name
Human Ortholog
Description Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0.05 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.05 0.05 0 0 – 0.05 0 0 0.06 0.09 0.15 0 0.08 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.14 0.29 0.25 0.3 0.54 0.53 0.52 – 0.58 0.67 0.57 0 0 0 0.16 0.11 0 0 0 0 0 0 0
Nucleus 0.9 0.79 0.77 0.79 0.7 0.69 0.66 0.62 – 0.61 0.65 0.63 0.91 0.87 0.8 0.88 0.85 0.85 0.68 0.63 0.62 0.64 0.67 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.08 0.05 0 0.05 0.05 0.09 0.11 – 0.12 0.12 0.16 0 0 0 0.07 0.11 0.08 0.14 0.14 0.16 0.12 0.14 0.14
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 – 0 0 0 0.07 0.05 0 0 0 0 0 0.05 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0.1 0.09 0.11 0.09 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2
Bud 0 0 0 0 2 7 10 11 – 8 12 13 0 0 0 0 0 0 3 2 0 4 5 5
Bud Neck 1 0 0 0 0 1 1 2 – 1 0 0 0 0 1 0 0 0 1 0 2 1 1 6
Bud Site 0 0 1 3 0 19 21 15 – 30 33 19 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 2 10 23 26 32 35 32 – 28 17 22 21 46 92 2 6 2 2 1 5 3 3 3
Endoplasmic Reticulum 1 0 1 0 0 1 0 0 – 0 0 1 0 1 1 0 0 1 0 0 0 2 2 3
Endosome 7 2 5 4 8 1 4 2 – 0 1 1 2 11 6 0 0 1 12 3 14 3 6 6
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 1 0 1 1 0 1 4 1 1 1 1 3
Mitochondria 23 16 114 87 142 331 451 424 – 357 390 389 2 3 0 13 8 2 5 3 5 3 3 5
Nucleus 333 91 301 275 337 417 562 505 – 378 376 431 322 440 476 73 61 52 305 91 267 172 293 305
Nuclear Periphery 1 1 0 3 5 5 9 3 – 10 7 10 0 0 2 1 0 1 1 0 1 1 0 1
Nucleolus 6 9 20 10 22 32 80 89 – 73 72 110 8 8 13 6 8 5 62 19 71 32 59 67
Peroxisomes 0 0 1 1 0 0 0 0 – 1 0 0 0 3 0 0 0 0 0 0 0 0 1 0
SpindlePole 14 2 20 11 5 6 13 10 – 20 11 9 25 25 26 3 2 1 10 6 17 9 14 13
Vac/Vac Membrane 7 6 3 3 22 5 9 15 – 7 6 13 2 4 7 0 3 1 33 14 38 29 39 51
Unique Cell Count 371 115 390 347 480 608 847 808 619 578 681 355 506 595 83 72 61 449 147 434 271 439 482
Labelled Cell Count 403 129 476 420 569 857 1195 1108 913 925 1018 383 541 625 99 88 67 449 147 434 271 439 482


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.0 4.2 4.3 4.2 4.0 3.7 3.9 – 3.5 3.5 3.7 6.5 6.5 6.5 7.0 7.0 7.3 6.6 6.9 7.1
Std Deviation (1e-4) 1.4 0.9 0.9 1.5 1.2 1.1 1.2 1.4 – 0.9 1.0 1.0 1.2 1.7 1.5 1.2 1.3 1.4 1.7 1.5 1.8
Intensity Change (Log2) – – – 0.03 0.02 -0.07 -0.18 -0.09 – -0.27 -0.24 -0.15 0.64 0.65 0.64 0.75 0.75 0.81 0.67 0.73 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 2.7 2.1 2.1 1.4 1.2 – 1.6 0.3 0.6 2.3 4.0 6.5 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 1.0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -10.8 -12.6 -14.0 -2.5 -3.2 -4.3
Nucleus 0.7 -2.3 -2.9 -3.9 -5.1 – -5.3 -4.0 -4.7 5.0 3.8 1.1 2.2 1.4 1.4
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 -2.1 -3.0 -2.5 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 1.1 -0.1 -0.6 0 0 0
Vacuole 0 3.3 0 0 1.4 – 0 0 1.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1185 2.2344 1.9126 1.5721 1.6861 1.9813 3.9071 3.2158 3.4074 3.1176 3.5868 3.5601 1.2312 1.1328 0.5772 1.3417 0.7475 1.0227
Actin 0.0265 0.0006 0.0111 0.0197 0.0081 0.0003 0.012 0.0003 0.0056 0 0.0009 0 0.0065 0 0.0095 0 0 0
Bud 0.0008 0.0001 0.0005 0.0001 0.0007 0.0001 0.0008 0 0.0001 0 0.0003 0 0.0003 0.0002 0.0001 0 0 0.0001
Bud Neck 0.0026 0.0001 0.0016 0.0005 0.0009 0.0012 0.0073 0.0001 0.0003 0.0001 0.0139 0.0009 0.0004 0.0004 0.0008 0 0.0012 0.0025
Bud Periphery 0.0014 0.0003 0.0012 0.0001 0.0032 0.0003 0.0039 0 0.0002 0 0.001 0 0.0002 0.0004 0.0002 0 0.0001 0.0002
Bud Site 0.007 0.0003 0.0069 0.0006 0.0021 0.0002 0.0021 0.0001 0.0004 0 0.0015 0 0.0023 0.0004 0.0005 0 0.0001 0.0002
Cell Periphery 0.0004 0 0.0002 0 0.0008 0.0001 0.0019 0 0.0001 0 0.0003 0 0.0001 0.0001 0 0 0 0.0001
Cytoplasm 0.0066 0.0001 0.0004 0.0014 0.0056 0.0004 0.0058 0.0019 0.0005 0.0001 0.0006 0.0004 0.0447 0.0001 0.0048 0 0 0.0001
Cytoplasmic Foci 0.011 0.0004 0.0038 0.0013 0.0063 0.0016 0.0066 0.0063 0.0027 0 0.0011 0.0002 0.0823 0 0.0099 0 0 0
Eisosomes 0.0005 0 0.0001 0.0001 0.0006 0 0.0005 0 0 0 0 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.004 0 0.0011 0.0002 0.0028 0.0003 0.0132 0.0012 0.0002 0 0.0004 0.0002 0.003 0 0.0001 0 0 0
Endosome 0.0262 0.0007 0.0121 0.003 0.0156 0.0028 0.0221 0.0038 0.0039 0 0.0036 0.0023 0.0198 0.0001 0.006 0 0 0
Golgi 0.0056 0.0008 0.0072 0.0004 0.0048 0.0004 0.0088 0.0003 0.0046 0 0.0016 0.0002 0.0054 0 0.0159 0 0 0
Lipid Particles 0.032 0.0002 0.0145 0.0014 0.0448 0.0033 0.0052 0.0032 0.0026 0 0.0022 0.0018 0.0114 0 0.0069 0 0 0
Mitochondria 0.0114 0.0073 0.0159 0.0005 0.0347 0.002 0.0141 0.0001 0.0104 0.0001 0.0009 0.0001 0.0012 0.0007 0.0058 0.0002 0.0003 0.0001
None 0.0145 0.0001 0.0003 0.0081 0.0207 0.0007 0.0093 0.0007 0.0001 0 0.0003 0.0005 0.0069 0 0.0029 0 0 0
Nuclear Periphery 0.0232 0.0007 0.0042 0.008 0.0201 0.0039 0.0245 0.0019 0.0015 0.0012 0.0047 0.0029 0.0233 0.0006 0.0008 0.0002 0.0018 0.0002
Nucleolus 0.03 0.0414 0.0206 0.0325 0.0545 0.0856 0.0324 0.0342 0.0221 0.0252 0.1145 0.0706 0.0422 0.059 0.0432 0.0254 0.1947 0.1006
Nucleus 0.7667 0.9425 0.8894 0.91 0.7216 0.8877 0.8008 0.9393 0.9396 0.9721 0.8321 0.9163 0.6725 0.9366 0.8657 0.9738 0.7867 0.8949
Peroxisomes 0.0061 0.0006 0.0038 0.0009 0.0162 0.0008 0.001 0.0003 0.002 0 0.0005 0 0.019 0 0.0173 0 0 0
Punctate Nuclear 0.0161 0.0034 0.0023 0.0098 0.0248 0.0068 0.0109 0.0061 0.0023 0.001 0.0175 0.0032 0.0571 0.0005 0.0091 0.0002 0.0145 0.0009
Vacuole 0.0048 0.0001 0.0017 0.0012 0.0076 0.0011 0.0104 0.0001 0.0005 0 0.0018 0.0001 0.001 0.0006 0.0001 0 0.0002 0.0003
Vacuole Periphery 0.0026 0.0001 0.0012 0.0003 0.0036 0.0006 0.0063 0 0.0003 0 0.0003 0.0002 0.0005 0.0003 0.0001 0 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.2236 15.242 7.5402 12.2546 18.159 13.3893 19.6309 17.4597 12.2164 18.6908
Translational Efficiency 1.0377 1.1395 1.2723 0.4941 0.8179 1.176 0.8886 0.7222 1.0847 0.884

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1185 1362 2592 1746 1061 1549 1873 1531 2246 2911 4465 3277

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 647.52 760.92 851.33 910.12 606.79 759.85 937.94 885.26 628.28 760.35 887.66 898.51
Standard Deviation 361.09 102.42 107.56 136.15 78.87 100.93 116.06 150.13 268.60 101.63 119.14 143.39
Intensity Change Log 2 — 0.232820 0.394794 0.491132 — 0.324517 0.628298 0.544904 — 0.277908 0.512477 0.517395

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000224 0.000845 0.000459 0.001189 0.000361 0.000380 0.000687 0.001019 0.000289 0.000597 0.000554 0.001110
Bud Neck 0.002999 0.005731 0.024844 0.023711 0.003709 0.004616 0.022328 0.021803 0.003334 0.005137 0.023789 0.022820
Bud Site 0.001128 0.001822 0.001483 0.005656 0.002122 0.000867 0.001742 0.003464 0.001597 0.001314 0.001591 0.004632
Cell Periphery 0.000206 0.000229 0.000054 0.000161 0.000262 0.000100 0.000044 0.000158 0.000233 0.000160 0.000050 0.000160
Cytoplasm 0.124475* 0.015227 0.002949 0.010969 0.088611 0.006686 0.001066 0.006756 0.107533* 0.010682 0.002159 0.009001
Cytoplasmic Foci 0.029943 0.004125 0.000108 0.000726 0.016593 0.002739 0.000036 0.000255 0.023636 0.003387 0.000078 0.000506
Eisosomes 0.000047 0.000037 0.000023 0.000035 0.000057 0.000026 0.000034 0.000027 0.000052 0.000031 0.000028 0.000032
Endoplasmic Reticulum 0.001279 0.003989 0.002562 0.004130 0.001969 0.002383 0.002815 0.004585 0.001605 0.003135 0.002668 0.004342
Endosome 0.001938 0.001305 0.000290 0.001177 0.001648 0.001474 0.000253 0.000811 0.001801 0.001395 0.000274 0.001006
Golgi 0.002485 0.001517 0.000121 0.000695 0.001068 0.001577 0.000131 0.000305 0.001815 0.001549 0.000125 0.000513
Lipid Particles 0.006739 0.001859 0.000068 0.000273 0.002582 0.000976 0.000073 0.000140 0.004775 0.001389 0.000070 0.000211
Mitochondria 0.007414 0.006087 0.001138 0.002879 0.003322 0.004561 0.001127 0.002064 0.005481 0.005275 0.001134 0.002498
Mitotic Spindle 0.005924 0.004520 0.003721 0.014131 0.001570 0.004488 0.001874 0.005999 0.003868 0.004503 0.002946 0.010332
None 0.075208 0.022316 0.002921 0.006946 0.078357 0.009774 0.001778 0.009642 0.076695 0.015642 0.002441 0.008206
Nuclear Periphery 0.000815 0.001202 0.000520 0.001287 0.000670 0.001333 0.000353 0.000870 0.000747 0.001271 0.000450 0.001092
Nuclear Periphery Foci 0.002200 0.002032 0.000158 0.000800 0.002588 0.001677 0.000124 0.000943 0.002383 0.001843 0.000144 0.000867
Nucleolus 0.048636 0.075096 0.047839 0.045850 0.069840 0.073339 0.076348 0.027486 0.058653 0.074161 0.059798 0.037270
Nucleus 0.682592* 0.847476* 0.909875* 0.872561* 0.718978* 0.879536* 0.887938* 0.911158* 0.699781* 0.864536* 0.900673* 0.890594*
Peroxisomes 0.003198 0.002211 0.000202 0.000630 0.002325 0.001711 0.000433 0.000351 0.002785 0.001945 0.000299 0.000500
Vacuole 0.002120 0.001589 0.000467 0.003819 0.002843 0.001188 0.000660 0.001815 0.002461 0.001375 0.000548 0.002883
Vacuole Periphery 0.000430 0.000786 0.000197 0.002375 0.000526 0.000570 0.000157 0.000348 0.000475 0.000671 0.000180 0.001428

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.74 -9.91 -15.14 -4.03 -11.39 -0.70 -10.92 -12.69 -10.99 -4.55 -5.55 -14.48 -19.71 -9.44 -11.99
Bud Neck -4.19 -23.03 -21.72 -19.08 -2.17 -1.24 -18.77 -16.07 -16.65 -1.19 -3.72 -29.41 -26.58 -25.49 -2.34
Bud Site -1.85 -2.46 -5.43 -4.12 -4.71 2.91 -0.27 -2.56 -4.66 -2.53 1.05 -1.22 -5.69 -6.29 -5.28
Cell Periphery -0.60 7.44 2.59 2.30 -4.41 4.67 9.69 4.71 -1.49 -7.70 2.65 12.06 5.00 0.85 -7.71
Cytoplasm 19.80 23.12 21.53 3.43 -6.60 15.80 17.15 16.06 0.79 -6.38 25.43 28.53 26.65 2.99 -8.81
Cytoplasmic Foci 10.89 13.20 13.04 4.49 -1.81 7.43 9.52 9.43 3.85 -2.48 13.20 16.20 16.02 5.86 -2.46
Eisosomes 4.22 10.58 3.75 -1.11 -9.58 5.36 3.75 4.78 -1.88 3.95 7.00 8.09 5.96 -2.43 -5.13
Endoplasmic Reticulum -7.09 -13.06 -18.36 -3.36 -11.21 -2.47 -8.76 -13.07 -10.40 -6.77 -7.61 -14.85 -22.36 -8.49 -13.11
Endosome 1.88 6.75 3.73 0.75 -3.83 0.69 8.08 3.86 1.41 -2.12 1.80 9.90 5.29 1.54 -4.18
Golgi 1.61 4.44 3.72 2.49 -2.65 -0.65 5.16 4.06 2.36 -1.40 0.91 5.77 4.81 3.32 -3.01
Lipid Particles 5.35 7.58 7.36 5.19 -5.81 6.04 10.62 10.34 6.57 -3.42 6.80 9.77 9.49 7.46 -6.73
Mitochondria 1.18 5.71 4.07 3.86 -5.21 -0.97 2.02 1.14 2.50 -3.71 0.39 5.83 3.97 4.27 -6.41
Mitotic Spindle 0.65 0.30 -4.97 -6.09 -5.99 -1.85 -1.79 -5.24 -3.11 -3.89 -0.41 -0.59 -6.86 -6.75 -7.06
None 11.23 17.05 16.09 7.84 -4.85 14.37 16.33 14.75 1.47 -7.04 18.35 23.60 21.82 7.47 -8.37
Nuclear Periphery -1.98 2.22 -8.65 -3.85 -11.15 -2.28 0.91 -2.91 0.65 -5.52 -2.94 1.92 -8.00 -1.87 -12.05
Nuclear Periphery Foci 1.11 7.20 3.54 2.30 -4.22 2.04 7.58 4.37 1.36 -3.00 2.28 10.43 5.57 2.37 -5.11
Nucleolus -5.10 0.55 2.30 8.03 2.21 -0.17 -1.29 9.10 11.22 12.24 -3.73 -0.14 8.03 13.32 9.98
Nucleus -16.52 -24.56 -19.04 -2.27 7.59 -16.40 -16.90 -18.21 -2.43 -3.10 -23.56 -29.67 -26.23 -2.86 3.85
Peroxisomes 2.32 7.50 6.78 4.02 -3.97 0.81 3.98 4.11 3.05 1.51 2.22 8.17 7.72 4.83 -3.13
Vacuole 1.24 4.65 -2.82 -3.62 -5.47 3.23 4.50 1.54 -1.64 -2.66 3.28 6.51 -1.38 -3.96 -6.11
Vacuole Periphery -3.08 4.31 -1.53 -0.90 -1.95 -0.26 2.70 1.37 1.76 -1.98 -1.90 4.27 -1.19 -0.57 -2.15
Standard name
Human Ortholog
Description Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions
Localization
Cell Percentages nucleus (88%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Snf5

Snf5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snf5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available