Standard name
Human Ortholog
Description Heavy metal ion homeostasis protein; facilitates trafficking of Smf1p and Smf2p metal transporters to vacuole where they are degraded; acts as an adaptor protein with Rsp5p in the regulated endocytosis of Smf1p and is itself ubiquitylated by Rsp5p; controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.61 0.67 0.79 0.84 0.91 0.97 0.92 0.91 0.93 0.92 0.93 0.89 0.63 0.57 0.61 0.75 0.73 0.72 0.05 0 0 0 0.07 0.05
Endoplasmic Reticulum 0.19 0.16 0.15 0.06 0 0 0 0 0 0 0 0 0.27 0.34 0.34 0 0 0 0.33 0.41 0.34 0.27 0.28 0.24
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0 0.05 0 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.34 0.18 0.08 0.21 0.09 0 0 0.06 0.05 0.08 0.05 0.08 0.12 0.09 0.06 0.21 0.32 0.32 0.53 0.45 0.57 0.64 0.6 0.66
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 2 0 0 0 3 0 0 0 1 5 6 1 0 0 1 2 0 0 0 1
Bud 0 1 1 1 4 18 12 19 13 14 16 28 0 3 2 0 0 0 0 0 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 1 1 4 0 1 2 6 1 11 20 1 1 1 0 0 0 0 0 0
Cytoplasm 75 87 66 224 407 399 578 623 507 494 451 601 127 211 282 78 95 61 8 7 4 7 24 16
Endoplasmic Reticulum 23 21 13 17 10 1 3 3 1 2 2 5 54 126 156 0 2 2 50 70 36 50 104 86
Endosome 1 0 0 3 1 0 2 1 1 3 0 1 1 2 3 4 0 0 2 4 1 1 3 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 1 1
Mitochondria 1 2 3 4 6 14 37 22 28 18 31 58 0 0 0 4 1 0 0 1 1 2 4 2
Nucleus 1 2 0 1 3 4 6 11 1 2 1 4 5 1 1 9 6 2 0 0 0 0 0 0
Nuclear Periphery 0 2 0 0 2 1 14 5 0 6 0 2 0 0 0 1 1 0 1 1 0 1 1 2
Nucleolus 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 42 23 7 57 39 16 28 43 29 43 25 51 24 35 27 22 42 27 79 76 60 119 219 238
Unique Cell Count 123 129 84 267 445 413 625 682 544 537 483 674 202 370 463 104 130 85 150 170 106 187 369 359
Labelled Cell Count 145 138 90 308 474 455 681 733 583 583 532 756 213 396 498 121 148 93 150 170 106 187 369 359


Endoplasmic Reticulum, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 7.9 6.1 6.6 5.5 4.7 4.6 4.8 4.6 4.6 4.4 4.4 8.1 7.7 7.6 8.7 9.1 9.5 8.4 9.1 9.1
Std Deviation (1e-4) 1.3 1.3 1.2 4.1 1.3 1.1 1.5 1.4 1.4 1.2 1.1 1.1 1.3 1.0 1.0 1.9 2.0 1.9 1.3 1.8 1.3
Intensity Change (Log2) – – – 0.12 -0.15 -0.36 -0.42 -0.36 -0.4 -0.4 -0.47 -0.46 0.41 0.34 0.33 0.51 0.58 0.64 0.46 0.58 0.58

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 3.5 6.1 4.1 3.7 4.4 3.8 4.4 2.8 -2.6 -3.7 -3.1 -0.6 -0.9 -1.0
Endoplasmic Reticulum -2.6 0 0 0 0 0 0 0 0 2.0 3.3 3.3 -4.2 -3.9 -3.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.7 0.1 0 0 -0.7 0 -0.1 0 -0.2 0.9 0.3 -0.9 2.4 4.1 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.159 4.3452 3.3479 3.5135 3.1586 3.9977 2.8367 3.8637 3.4912 3.6643 2.8936 3.5909 1.9719 3.0407 2.2418 2.2561 1.7546 2.1404
Actin 0.0186 0.0006 0.0224 0.0042 0.0017 0.0011 0.0408 0.0002 0.0012 0.0083 0.0002 0.0082 0.0044 0 0.0163 0.0001 0.0607 0
Bud 0.0013 0.0121 0.0017 0.0218 0.0005 0.0011 0.0004 0.0004 0.0005 0.0006 0.0016 0.0002 0.0002 0.0001 0.0002 0.0005 0.0009 0.0005
Bud Neck 0.0061 0.0016 0.0083 0.0055 0.0028 0.0094 0.0039 0.0004 0.0009 0.0014 0.0015 0.0014 0.0005 0.0008 0.0004 0.006 0.002 0.0031
Bud Periphery 0.0013 0.0024 0.0028 0.0069 0.0015 0.0013 0.001 0.0006 0.0014 0.0015 0.0024 0.0009 0.0004 0.0002 0.0004 0.0013 0.0019 0.0015
Bud Site 0.0115 0.0172 0.0161 0.01 0.001 0.0019 0.0057 0.0025 0.0061 0.0037 0.0057 0.0017 0.0011 0.0007 0.0006 0.0045 0.0023 0.0023
Cell Periphery 0.0028 0.0036 0.0029 0.0024 0.0039 0.0036 0.0096 0.005 0.0038 0.0034 0.0076 0.0038 0.0026 0.0055 0.002 0.0043 0.0009 0.0042
Cytoplasm 0.099 0.2146 0.1509 0.2277 0.182 0.1738 0.082 0.2234 0.1367 0.1111 0.0724 0.0879 0.1462 0.144 0.1431 0.0901 0.2377 0.0886
Cytoplasmic Foci 0.0616 0.0227 0.0283 0.0465 0.0188 0.0314 0.0181 0.009 0.0194 0.0394 0.0279 0.0078 0.0088 0.0084 0.0081 0.0131 0.0176 0.007
Eisosomes 0.0005 0 0.0001 0.0001 0 0 0.0011 0 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0004 0
Endoplasmic Reticulum 0.2826 0.2974 0.2457 0.1611 0.1515 0.2681 0.4799 0.4057 0.4466 0.2178 0.3558 0.4098 0.4832 0.493 0.4616 0.1601 0.0652 0.314
Endosome 0.1239 0.0698 0.0992 0.0796 0.0908 0.0685 0.0625 0.0656 0.1049 0.1353 0.1739 0.0522 0.0514 0.0385 0.0667 0.1262 0.1176 0.0536
Golgi 0.0163 0.0016 0.0084 0.0062 0.0093 0.0029 0.0189 0.0008 0.0068 0.0108 0.0018 0.0011 0.0021 0.0005 0.0062 0.002 0.019 0.0005
Lipid Particles 0.0456 0.0058 0.0112 0.0118 0.0159 0.0111 0.0294 0.0154 0.0137 0.0265 0.015 0.0067 0.0139 0.0149 0.0175 0.0095 0.0329 0.0038
Mitochondria 0.0157 0.0023 0.0068 0.0291 0.068 0.0023 0.0037 0.0004 0.0052 0.0379 0.0033 0.0009 0.0093 0.0002 0.0023 0.0007 0.0139 0.0006
None 0.0762 0.0525 0.0786 0.0465 0.0112 0.0181 0.0196 0.045 0.0279 0.0271 0.0043 0.0303 0.0145 0.0031 0.0015 0.0008 0.0019 0.0014
Nuclear Periphery 0.0292 0.0387 0.0396 0.0313 0.0559 0.0406 0.0304 0.0295 0.0279 0.0176 0.0404 0.0363 0.0571 0.0301 0.0569 0.0338 0.0533 0.0381
Nucleolus 0.0064 0.0024 0.003 0.0152 0.0004 0.0035 0.0003 0.0004 0.0006 0.0004 0.0173 0.0002 0.0016 0.0002 0.0001 0.0008 0.0005 0.0007
Nucleus 0.0156 0.0151 0.0206 0.02 0.0093 0.0213 0.003 0.005 0.0045 0.004 0.0067 0.0058 0.0098 0.0041 0.0061 0.0062 0.0091 0.01
Peroxisomes 0.0165 0.0003 0.0037 0.0094 0.0151 0.0008 0.0095 0.0001 0.0099 0.0112 0.0014 0.0002 0.0076 0.0001 0.0046 0.0002 0.0129 0.0001
Punctate Nuclear 0.0215 0.0055 0.005 0.021 0.0049 0.0057 0.0037 0.0006 0.0007 0.0032 0.0024 0.0003 0.0041 0.0004 0.0028 0.0006 0.0036 0.0003
Vacuole 0.1403 0.2275 0.2313 0.2334 0.3413 0.322 0.1717 0.1845 0.1709 0.3188 0.2462 0.3283 0.1724 0.2465 0.1887 0.5049 0.3141 0.4528
Vacuole Periphery 0.0075 0.0062 0.0134 0.0103 0.0144 0.0115 0.005 0.0055 0.0106 0.0198 0.0121 0.0159 0.0088 0.0085 0.0138 0.0346 0.0315 0.0168

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.5646 8.1687 9.0923 18.2151 12.5882 7.9913 22.4553 21.6014 15.6405 17.4894
Translational Efficiency 2.1206 3.3797 2.5208 1.1341 1.6994 4.0364 1.3657 1.0189 1.7601 1.7852

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2603 1208 2983 1002 1169 1505 584 1788 3772 2713 3567 2790

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 747.99 879.79 1017.28 1061.60 842.82 788.09 1053.09 1084.45 777.38 828.92 1023.14 1076.24
Standard Deviation 97.81 119.79 123.17 194.27 105.21 133.48 174.61 166.09 109.34 135.46 133.62 177.07
Intensity Change Log 2 — 0.234140 0.443626 0.505149 — -0.096864 0.321332 0.363667 — 0.068254 0.380127 0.431922

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000249 0.000151 0.000306 0.004843 0.000071 0.000811 0.002289 0.002782 0.000194 0.000517 0.000631 0.003522
Bud Neck 0.007088 0.002686 0.004063 0.007377 0.002012 0.005801 0.005762 0.004850 0.005515 0.004414 0.004342 0.005758
Bud Site 0.004007 0.003279 0.005704 0.034005 0.001215 0.007004 0.019541 0.014779 0.003141 0.005346 0.007970 0.021684
Cell Periphery 0.000838 0.000532 0.000627 0.004509 0.000746 0.001050 0.003341 0.003005 0.000809 0.000819 0.001071 0.003545
Cytoplasm 0.167795 0.122861 0.196508 0.128186 0.078790 0.097263 0.126869 0.111747 0.140211 0.108660 0.185106 0.117651
Cytoplasmic Foci 0.047601 0.036992 0.006020 0.023232 0.024384 0.061486 0.011259 0.014017 0.040406 0.050580 0.006877 0.017327
Eisosomes 0.000187 0.000077 0.000062 0.000324 0.000100 0.000222 0.000348 0.000626 0.000160 0.000158 0.000108 0.000517
Endoplasmic Reticulum 0.389678 0.480075 0.495887 0.560993 0.572608 0.378684 0.634081 0.723048 0.446371 0.423830 0.518512 0.664848
Endosome 0.030326 0.023594 0.008134 0.024917 0.019394 0.046030 0.016297 0.015413 0.026938 0.036040 0.009471 0.018826
Golgi 0.005122 0.002641 0.000284 0.006033 0.002030 0.006622 0.004428 0.007387 0.004164 0.004849 0.000962 0.006901
Lipid Particles 0.033158 0.015326 0.008465 0.038255 0.016857 0.030673 0.020785 0.030360 0.028106 0.023839 0.010482 0.033196
Mitochondria 0.005423 0.002372 0.001235 0.001770 0.000752 0.004538 0.001430 0.002338 0.003975 0.003573 0.001267 0.002134
Mitotic Spindle 0.000703 0.000270 0.000632 0.006652 0.000027 0.000218 0.002376 0.001993 0.000493 0.000241 0.000917 0.003666
None 0.003944 0.000969 0.001101 0.002273 0.000484 0.001396 0.001948 0.002722 0.002872 0.001206 0.001239 0.002560
Nuclear Periphery 0.003705 0.003545 0.065793 0.006550 0.003588 0.002906 0.003262 0.001256 0.003669 0.003191 0.055555 0.003158
Nuclear Periphery Foci 0.002940 0.005951 0.036740 0.041958 0.004676 0.004182 0.036382 0.016891 0.003478 0.004970 0.036681 0.025894
Nucleolus 0.001021 0.000384 0.000175 0.000190 0.000944 0.000954 0.000130 0.000182 0.000997 0.000700 0.000168 0.000185
Nucleus 0.023943 0.012862 0.105200 0.018127 0.007824 0.010715 0.022264 0.011273 0.018948 0.011671 0.091622 0.013735
Peroxisomes 0.002559 0.001090 0.000162 0.001168 0.000490 0.001832 0.000360 0.000873 0.001918 0.001502 0.000195 0.000979
Vacuole 0.264024 0.278349 0.062403 0.084932 0.258612 0.326252 0.085846 0.032327 0.262347 0.304922 0.066241 0.051220
Vacuole Periphery 0.005691 0.005997 0.000500 0.003706 0.004397 0.011362 0.001002 0.002128 0.005290 0.008973 0.000582 0.002695

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.28 -1.20 -4.02 -4.11 -3.95 -1.86 -2.59 -5.92 -3.42 -0.41 -1.42 -3.06 -6.60 -5.56 -5.44
Bud Neck 6.15 3.58 -0.48 -4.65 -2.99 -4.95 -4.66 -4.33 0.78 1.20 1.82 1.39 -0.70 -2.34 -1.96
Bud Site 1.00 -2.12 -8.16 -8.34 -7.49 -6.01 -5.89 -8.71 -4.47 1.60 -3.29 -5.65 -11.05 -9.44 -7.14
Cell Periphery 4.77 2.62 -5.85 -6.35 -6.17 -1.47 -3.81 -6.51 -4.99 0.66 -0.08 -2.21 -8.65 -8.20 -7.16
Cytoplasm 7.64 -5.89 4.77 -1.20 8.74 -3.47 -6.17 -6.33 -2.95 2.04 7.73 -10.65 4.45 -2.55 13.67
Cytoplasmic Foci 3.68 23.89 9.33 4.35 -9.24 -11.03 5.85 4.99 15.10 -1.67 -4.39 24.71 14.02 15.11 -10.25
Eisosomes 6.96 8.24 -3.89 -7.47 -7.94 -2.01 -3.75 -3.98 -2.81 -1.86 0.08 3.05 -4.18 -3.96 -4.74
Endoplasmic Reticulum -7.39 -10.35 -10.53 -3.65 -3.97 14.25 -2.10 -10.99 -27.75 -6.09 2.54 -7.13 -22.72 -23.28 -16.33
Endosome 4.34 20.51 2.66 -0.71 -9.50 -12.19 1.69 2.59 13.47 0.39 -6.30 20.10 6.59 10.98 -9.09
Golgi 4.84 14.13 -0.57 -2.59 -4.74 -5.76 -1.58 -6.24 -0.61 -1.91 -1.32 9.66 -3.65 -2.35 -8.20
Lipid Particles 11.35 18.60 -2.10 -7.64 -10.02 -6.19 -1.90 -5.83 -0.24 -3.25 2.93 15.78 -3.24 -5.18 -12.95
Mitochondria 4.16 9.70 6.96 0.93 -1.42 -5.10 -1.00 -4.28 2.78 -1.29 0.71 8.34 4.87 2.70 -3.06
Mitotic Spindle 1.16 -0.55 -3.55 -3.86 -3.42 -3.45 -1.65 -4.23 -3.84 0.06 1.00 -1.62 -4.60 -5.19 -3.49
None 3.92 3.69 1.57 -1.33 -1.18 -1.94 -1.01 -3.27 -1.69 -0.53 2.98 2.74 0.51 -2.25 -2.08
Nuclear Periphery 0.26 -26.63 -2.67 -2.60 23.46 1.14 0.53 4.91 3.83 5.19 1.16 -26.10 1.14 0.07 26.10
Nuclear Periphery Foci -4.08 -25.01 -13.06 -11.77 -1.72 0.79 -10.63 -8.88 -9.22 5.97 -3.47 -26.95 -16.06 -14.61 5.59
Nucleolus 8.72 12.98 12.51 4.13 -0.35 -0.08 1.78 1.67 4.03 -1.10 1.55 5.58 5.43 4.71 -0.37
Nucleus 9.12 -28.34 2.36 -3.40 26.63 -2.35 -6.28 -3.15 -0.88 4.74 8.12 -29.48 4.12 -2.74 30.49
Peroxisomes 2.18 4.19 2.28 -0.19 -4.55 -6.12 1.20 -3.99 4.08 -3.92 0.93 4.35 2.30 2.34 -7.83
Vacuole -1.48 33.89 22.61 18.98 -4.87 -5.84 14.81 26.09 34.68 8.40 -5.81 37.92 40.37 39.63 5.12
Vacuole Periphery -0.58 19.72 2.75 2.79 -4.12 -9.94 8.69 3.99 11.97 -2.23 -8.25 21.45 6.01 11.18 -4.99
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Heavy metal ion homeostasis protein; facilitates trafficking of Smf1p and Smf2p metal transporters to vacuole where they are degraded; acts as an adaptor protein with Rsp5p in the regulated endocytosis of Smf1p and is itself ubiquitylated by Rsp5p; controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification
Localization
Cell Percentages ER (49%), vacuole (11%), mixed (39%)
Cell Cycle Regulation Yes
vacuole - MA (8.9e-06)
Subcompartmental Group N/A

Bsd2

Bsd2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bsd2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available