Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF) for Cdc42p; activates Cdc42p in early G1, accounting for the first stage of biphasic activation, with Cdc24p accounting for the second stage in late G1; involved in the Cdc42p-mediated assembly of the axial landmark that dictates the site for the next round of budding, resulting in the axial budding pattern observed in haploids; localizes with septins to the bud neck contractile ring in mitosis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.15 0.12 0.12 0.08 0 0 0.06 0.08 0.05 0 0.06 0 0.21 0.24 0.27 0.09 0.07 0.06 0.13 0.09 0.11 0.06 0.06 0.05
Bud 0.06 0.1 0.2 0.14 0.21 0.23 0.17 0.19 0.23 0.19 0.21 0.19 0.08 0.11 0.18 0.05 0.05 0.05 0.18 0.2 0.16 0.21 0 0
Bud Neck 0.19 0.15 0.29 0.2 0.27 0.25 0.18 0.18 0.15 0.15 0.14 0.11 0.13 0.19 0.18 0.23 0.28 0.22 0.47 0.49 0.54 0.3 0.12 0.09
Bud Site 0.35 0.37 0.39 0.42 0.28 0.27 0.25 0.21 0.2 0.17 0.2 0.19 0.42 0.33 0.35 0.27 0.21 0.26 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.05 0.12 0 0 0.28 0.58 0.65
Cytoplasm 0.07 0.16 0.1 0.13 0.14 0.14 0.2 0.17 0.23 0.23 0.26 0.25 0.13 0.13 0.1 0.11 0.1 0.13 0 0 0 0.09 0.13 0.13
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.29 0.14 0.23 0.11 0.19 0.24 0.24 0.38 0.42 0.33 0.4 0.06 0 0.09 0.07 0 0.06 0 0 0 0 0 0
Nucleus 0.07 0.1 0.08 0.12 0.12 0.16 0.1 0.11 0.08 0.07 0.08 0.07 0 0 0 0.11 0.12 0.15 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0.1 0.06 0.07 0 0.06 0.06 0.05 0 0 0 0 0.07 0.07 0 0 0 0 0 0
Peroxisomes 0.19 0.09 0 0.12 0.07 0.08 0.09 0.07 0.07 0.05 0.06 0.07 0.23 0.25 0.22 0.07 0.1 0.07 0 0 0 0 0 0
SpindlePole 0.18 0.09 0.22 0.23 0.27 0.24 0.23 0.19 0.13 0.15 0.14 0.16 0.17 0.13 0.14 0.27 0.31 0.29 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 15 33 17 16 4 34 47 17 11 19 8 33 47 57 47 27 32 13 12 30 5 12 13
Bud 5 12 54 31 96 100 97 110 86 69 71 85 13 22 37 28 21 25 18 27 47 18 3 3
Bud Neck 17 19 78 43 121 111 100 103 56 55 48 50 21 37 37 116 112 111 49 69 158 26 25 25
Bud Site 31 46 106 90 126 118 142 121 74 62 65 89 67 65 73 140 83 128 – – – – – –
Cell Periphery 4 2 2 2 3 7 6 12 13 10 6 14 4 7 3 30 27 26 12 5 4 24 116 177
Cytoplasm 6 20 28 28 62 61 109 99 84 86 87 116 21 25 21 54 38 65 2 5 8 8 25 34
Endoplasmic Reticulum 1 0 3 0 3 2 3 5 2 2 1 1 2 3 0 7 9 1 0 1 3 1 5 9
Endosome 0 0 0 0 0 1 1 0 0 0 0 1 0 3 2 4 6 3 1 3 8 1 2 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 2 0 0 0 0 1 0
Mitochondria 1 36 39 49 48 85 132 141 140 158 111 185 9 3 18 34 17 28 1 2 4 0 2 2
Nucleus 6 13 23 25 56 72 55 61 28 25 26 31 7 8 3 56 49 72 0 1 3 0 0 0
Nuclear Periphery 0 2 1 2 3 1 3 5 0 0 2 1 0 2 2 2 1 0 1 1 2 0 0 0
Nucleolus 6 5 3 8 11 42 31 42 16 22 19 23 4 3 8 20 28 34 0 0 0 0 0 0
Peroxisomes 17 11 12 26 33 36 49 41 24 20 21 30 36 49 46 35 38 36 0 2 6 0 0 0
SpindlePole 16 11 59 50 119 107 129 112 47 56 46 72 27 26 30 140 125 145 1 4 6 0 0 1
Vac/Vac Membrane 0 1 2 1 3 2 4 3 0 1 1 2 0 1 2 8 6 5 0 3 4 2 3 2
Unique Cell Count 89 125 273 214 449 438 557 579 367 372 332 457 159 197 209 511 398 496 106 141 294 90 201 272
Labelled Cell Count 123 193 443 372 700 749 895 902 587 577 523 708 244 301 339 722 591 713 106 141 294 90 201 272


Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 4.8 4.6 4.1 4.5 4.0 3.9 4.0 3.3 3.5 3.5 3.2 6.4 5.9 5.0 8.7 9.6 9.6 6.6 6.5 6.6
Std Deviation (1e-4) 1.4 1.0 1.6 1.2 1.3 1.2 1.1 1.2 1.0 1.3 1.3 1.2 1.6 1.4 1.2 1.3 1.7 1.9 1.1 1.4 1.3
Intensity Change (Log2) – – – -0.17 -0.02 -0.2 -0.25 -0.22 -0.47 -0.4 -0.4 -0.52 0.47 0.35 0.12 0.92 1.07 1.06 0.52 0.49 0.53

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2658 2.6091 2.5289 1.9518 2.1939 2.9086 2.9439 3.3379 3.0276 2.896 2.7818 3.4331 3.8342 4.0926 3.8995 4.0732 3.7557 4.0965
Actin 0.1485 0.0288 0.082 0.0055 0.0008 0.0195 0.0694 0.0221 0.0181 0.0004 0.026 0.0303 0.0713 0.0203 0.0067 0.0052 0.0036 0.0967
Bud 0.0025 0.0054 0.0018 0.0011 0.0013 0.0008 0.0039 0.0121 0.0029 0.0003 0.0005 0.0007 0.0013 0.0045 0.0007 0.0005 0.0003 0.003
Bud Neck 0.2688 0.0744 0.554 0.8045 0.7889 0.9058 0.3149 0.0639 0.6567 0.981 0.9176 0.938 0.426 0.0521 0.678 0.9373 0.9917 0.8257
Bud Periphery 0.0044 0.0042 0.0031 0.0017 0.0021 0.0019 0.0097 0.0197 0.0043 0.0005 0.0008 0.0022 0.0015 0.0063 0.0011 0.001 0.0005 0.0165
Bud Site 0.3223 0.8343 0.2794 0.0325 0.0095 0.0084 0.389 0.8346 0.2919 0.0157 0.003 0.0059 0.4188 0.7031 0.2814 0.0505 0.0014 0.0061
Cell Periphery 0.0055 0.0082 0.0057 0.0014 0.0035 0.0014 0.0071 0.0047 0.0027 0.0007 0.0009 0.0009 0.0022 0.0185 0.0034 0.0015 0.0005 0.0107
Cytoplasm 0.0148 0.0047 0.0183 0.0005 0.0004 0.0006 0.0064 0.0047 0.0029 0.0001 0.0002 0.0003 0.0107 0.1069 0.0012 0.0004 0.0001 0.0179
Cytoplasmic Foci 0.0338 0.0043 0.0054 0.0032 0.0073 0.0049 0.0308 0.005 0.0063 0.0001 0.0047 0.0038 0.0046 0.0053 0.0099 0.0009 0.0003 0.0012
Eisosomes 0.0025 0.0002 0.0006 0.0006 0.0001 0.0001 0.0008 0.0005 0.0002 0 0.0002 0.0001 0.0004 0.0004 0.0008 0.0001 0 0.0004
Endoplasmic Reticulum 0.0063 0.0001 0.0011 0.0011 0 0.0003 0.0019 0.0003 0.0001 0 0.0002 0 0.0036 0.0025 0 0 0 0.0003
Endosome 0.0161 0.0009 0.0027 0.0112 0.0008 0.0031 0.0191 0.0014 0.001 0 0.0022 0.0014 0.0044 0.0024 0.0003 0.0001 0 0.0002
Golgi 0.0076 0.0006 0.0047 0.0042 0.0012 0.0024 0.0132 0.0027 0.0015 0 0.0037 0.0026 0.0007 0.0008 0.0006 0.0002 0.0001 0.0006
Lipid Particles 0.0326 0.0011 0.0054 0.0302 0.0045 0.0091 0.0175 0.0012 0.0008 0 0.0159 0.0066 0.0007 0.0006 0.0035 0.0001 0.0001 0.0001
Mitochondria 0.0083 0.0002 0.0037 0.0769 0.0016 0.0009 0.0145 0.0004 0.0017 0 0.0008 0.0009 0.0002 0.0005 0.0001 0 0 0.0001
None 0.0317 0.0003 0.0043 0.0026 0.0002 0.0005 0.0125 0.0117 0.0008 0 0.0007 0.0001 0.013 0.0618 0.0037 0.0002 0 0.0139
Nuclear Periphery 0.0237 0.0002 0.0054 0.0065 0 0.0032 0.017 0.0004 0 0 0.0007 0 0.0073 0.0009 0 0 0 0.0001
Nucleolus 0.004 0.0007 0.0012 0.0036 0.157 0.0078 0.003 0.0014 0.0011 0.0005 0.0026 0.0002 0.0013 0.001 0.0013 0.0011 0.0007 0.0002
Nucleus 0.0158 0.0006 0.0066 0.0018 0.0122 0.008 0.0051 0.0068 0.0005 0.0003 0.0006 0.0001 0.008 0.0034 0.0004 0.0004 0.0003 0.0017
Peroxisomes 0.0213 0.0078 0.0082 0.0034 0.0027 0.0067 0.0355 0.0038 0.0055 0 0.0157 0.0053 0.0006 0.0002 0.0031 0.0002 0.0001 0.0017
Punctate Nuclear 0.025 0.0024 0.0054 0.0049 0.0019 0.0138 0.0247 0.0014 0.0007 0.0001 0.0025 0.0004 0.0225 0.0005 0.0034 0.0002 0.0001 0.0001
Vacuole 0.0029 0.0198 0.0008 0.0014 0.0035 0.0005 0.0023 0.0011 0.0002 0.0001 0.0004 0.0001 0.0008 0.0075 0.0002 0.0001 0.0001 0.0027
Vacuole Periphery 0.0014 0.0008 0.0002 0.0013 0.0004 0.0002 0.0017 0.0001 0 0 0.0001 0 0.0002 0.0003 0 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.7055 29.525 23.1011 20.5993 12.3806 13.0242 34.5235 32.2788 22.7877 17.6549
Translational Efficiency 0.702 0.6874 0.7329 0.5748 0.5239 0.5149 0.5012 0.4564 0.5233 0.4313

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
344 1386 187 1525 1421 2038 819 2966 1765 3424 1006 4491

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 755.40 837.89 1126.27 968.24 726.56 822.91 945.70 1013.31 732.18 828.97 979.27 998.01
Standard Deviation 190.79 108.90 137.66 141.25 95.76 106.44 131.92 140.90 120.86 107.70 150.41 142.63
Intensity Change Log 2 — 0.149520 0.576240 0.358124 — 0.179653 0.380301 0.479922 — 0.164372 0.483497 0.419123

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006969 0.007857 0.029830 0.015191 0.005181 0.006609 0.003730 0.007884 0.005530 0.007114 0.008582 0.010365
Bud Neck 0.354397 0.392191 0.256807 0.271778 0.451513 0.463260 0.401982 0.290683 0.432585 0.434492 0.374997 0.284263
Bud Site 0.346012 0.451730 0.487444 0.590600 0.395417 0.411191 0.441651 0.617817 0.385788 0.427601 0.450163 0.608575
Cell Periphery 0.044363 0.018244 0.003580 0.004457 0.027059 0.028976 0.006330 0.014069 0.030431 0.024632 0.005819 0.010805
Cytoplasm 0.123005 0.044355 0.031068 0.040609 0.053034 0.034165 0.058775 0.043799 0.066671 0.038290 0.053625 0.042716
Cytoplasmic Foci 0.027004 0.019105 0.008393 0.005068 0.016723 0.012797 0.008182 0.001507 0.018727 0.015350 0.008221 0.002716
Eisosomes 0.007372 0.001247 0.000714 0.000530 0.001076 0.001482 0.000785 0.000570 0.002303 0.001387 0.000772 0.000556
Endoplasmic Reticulum 0.006107 0.000437 0.014330 0.000566 0.000305 0.000214 0.000728 0.000299 0.001436 0.000304 0.003256 0.000390
Endosome 0.007591 0.011464 0.002987 0.007028 0.008341 0.008700 0.003120 0.001819 0.008195 0.009819 0.003095 0.003588
Golgi 0.002028 0.005042 0.000360 0.001435 0.001951 0.002719 0.000798 0.000655 0.001966 0.003659 0.000717 0.000920
Lipid Particles 0.003052 0.000798 0.002348 0.000991 0.000830 0.000538 0.001022 0.000045 0.001263 0.000643 0.001268 0.000366
Mitochondria 0.004711 0.010073 0.000472 0.002539 0.008055 0.007269 0.001199 0.002929 0.007403 0.008404 0.001064 0.002797
Mitotic Spindle 0.000807 0.003292 0.008687 0.008007 0.001086 0.002276 0.003821 0.003350 0.001032 0.002687 0.004725 0.004931
None 0.003839 0.000576 0.000323 0.000113 0.001118 0.000271 0.000992 0.000204 0.001648 0.000395 0.000868 0.000174
Nuclear Periphery 0.000090 0.000046 0.000775 0.000119 0.000023 0.000015 0.000070 0.000013 0.000036 0.000028 0.000201 0.000049
Nuclear Periphery Foci 0.000744 0.001233 0.003837 0.001229 0.000177 0.000449 0.001090 0.000092 0.000287 0.000766 0.001600 0.000478
Nucleolus 0.000932 0.000866 0.000480 0.000137 0.000450 0.000168 0.001312 0.000094 0.000544 0.000450 0.001157 0.000109
Nucleus 0.037537 0.003166 0.056408 0.005560 0.006292 0.001462 0.017005 0.001659 0.012382 0.002152 0.024329 0.002984
Peroxisomes 0.005965 0.017701 0.006284 0.004814 0.008219 0.011089 0.006183 0.001521 0.007780 0.013766 0.006202 0.002639
Vacuole 0.016911 0.009661 0.084711 0.038147 0.012627 0.005757 0.040955 0.010392 0.013462 0.007337 0.049089 0.019817
Vacuole Periphery 0.000565 0.000915 0.000161 0.001081 0.000522 0.000593 0.000269 0.000599 0.000531 0.000723 0.000249 0.000763

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.47 -2.94 -3.52 -3.83 1.80 -1.45 1.38 -2.46 -1.18 -3.57 -1.85 -1.74 -4.71 -3.30 -1.23
Bud Neck -1.90 5.24 4.54 10.83 -2.43 -1.01 4.28 15.45 18.58 7.83 -0.22 5.81 16.42 20.94 6.72
Bud Site -5.86 -2.37 -12.95 -10.99 -6.39 -1.54 -2.66 -23.05 -23.77 -15.00 -4.74 -3.47 -25.83 -25.47 -15.85
Cell Periphery 4.24 6.79 6.70 8.39 -0.91 -0.72 9.85 5.91 7.37 -6.46 2.47 11.91 9.42 9.84 -5.62
Cytoplasm 6.24 6.03 6.18 -0.26 -0.95 4.34 -1.76 1.61 -3.67 3.12 6.59 1.10 4.86 -2.87 2.77
Cytoplasmic Foci 1.81 3.70 5.15 7.06 1.82 1.91 3.78 8.49 9.17 6.26 1.84 5.09 9.52 11.75 5.78
Eisosomes 3.32 3.62 3.74 2.27 1.28 -1.34 1.58 2.79 3.58 2.07 2.13 3.91 4.46 4.24 2.28
Endoplasmic Reticulum 2.44 -3.41 2.35 -1.63 6.07 1.92 -2.49 -0.08 -0.66 2.07 2.47 -3.66 2.15 -1.09 5.98
Endosome -2.34 2.65 0.39 2.81 -2.25 -0.26 5.40 7.04 6.56 3.10 -1.52 5.97 5.33 7.20 -0.36
Golgi -4.16 5.21 1.46 5.04 -3.31 -2.12 4.26 5.77 6.95 0.75 -4.63 5.46 4.95 8.17 -1.08
Lipid Particles 3.80 -0.51 3.17 -0.54 4.29 1.30 -0.41 4.55 3.24 1.69 2.89 -0.48 4.27 1.65 2.20
Mitochondria -2.85 3.15 1.60 5.01 -3.01 0.48 5.11 3.86 3.66 -2.30 -0.75 5.67 4.24 5.99 -2.82
Mitotic Spindle -4.18 -1.39 -5.17 -3.15 0.02 -2.08 -2.67 -5.06 -1.51 0.88 -3.95 -2.91 -6.97 -3.28 0.18
None 1.32 1.42 1.52 3.00 1.15 3.08 0.19 3.33 1.35 1.96 2.37 1.19 2.81 3.20 2.11
Nuclear Periphery 1.95 -2.02 -0.77 -1.67 1.95 1.27 -5.89 1.50 0.30 7.00 0.91 -2.69 -1.02 -1.45 2.49
Nuclear Periphery Foci -1.48 -1.76 -0.84 -0.01 1.51 -3.74 -3.72 3.87 5.00 4.06 -3.65 -3.15 -0.99 1.23 2.56
Nucleolus 0.15 1.15 2.66 1.86 2.08 1.39 -1.15 1.84 1.01 1.66 0.43 -1.02 2.61 2.07 1.77
Nucleus 5.66 -3.12 4.98 -3.20 5.99 5.24 -5.73 4.60 -1.47 7.82 7.09 -5.81 5.93 -3.14 9.13
Peroxisomes -4.96 0.12 0.65 6.32 0.59 -1.66 1.17 5.36 7.72 3.19 -4.09 1.16 4.75 10.17 2.77
Vacuole 2.69 -7.71 -7.18 -10.33 5.92 5.12 -8.01 0.49 -5.91 8.29 4.99 -10.77 -6.08 -11.46 8.62
Vacuole Periphery -1.39 2.54 -1.59 -0.43 -2.88 -0.23 1.62 -0.31 -0.02 -1.53 -0.88 2.13 -1.13 -0.14 -2.87
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF) for Cdc42p; activates Cdc42p in early G1, accounting for the first stage of biphasic activation, with Cdc24p accounting for the second stage in late G1; involved in the Cdc42p-mediated assembly of the axial landmark that dictates the site for the next round of budding, resulting in the axial budding pattern observed in haploids; localizes with septins to the bud neck contractile ring in mitosis
Localization
Cell Percentages bud neck (30%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bud3

Bud3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available