Standard name
Human Ortholog
Description Membrane protein localized to the shmoo tip; required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.09 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.17 0.07 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.82 0.96 0.97 0.97 0.91 0.81 0.84 0.82 0.81 0.75 0.78 0.74 1.0 0.99 0.99 – 0.96 0.97 0.54 0.79 0.6 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.07 0.07 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.06 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.05 0.08
Mitochondria 0 0 0 0 0 0.25 0.25 0.25 0.34 0.44 0.42 0.42 0 0 0 – 0 0 0 0 0.1 0.09 0.08 0.06
Nucleus 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0.07 0.06 0.05 0 0.05 0 0 0 0 0 0 – 0 0 0.22 0.09 0.14 0.32 0.6 0.63
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 8 5 13 27 33 14
Bud 0 2 1 3 0 10 6 12 15 12 14 11 0 0 0 – 0 0 10 4 9 50 56 32
Bud Neck 5 3 22 5 3 2 3 11 2 3 2 3 4 4 3 – 7 4 2 2 5 7 9 3
Bud Site 2 0 0 0 0 0 2 0 3 2 1 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 6 4 9 0 1 1 3 2 2 2 4 1 4 3 0 – 7 8 2 2 9 0 1 0
Cytoplasm 345 440 815 519 662 548 627 742 587 658 367 410 471 487 490 – 383 302 337 350 454 3 4 2
Endoplasmic Reticulum 1 2 0 0 0 0 0 1 0 0 0 1 0 1 1 – 22 23 43 0 8 10 27 22
Endosome 6 0 0 0 4 1 1 1 0 0 0 2 0 1 0 – 5 1 18 4 18 24 47 31
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 2 1 2 0 0 22 41 53
Mitochondria 7 9 5 14 23 170 187 226 248 381 198 230 0 0 0 – 8 11 26 16 79 27 70 41
Nucleus 33 0 11 5 18 20 18 31 8 7 4 5 0 1 2 – 0 1 0 0 3 0 4 5
Nuclear Periphery 15 0 1 0 0 1 9 7 2 1 0 1 0 0 0 – 0 0 2 1 1 1 16 18
Nucleolus 2 0 0 1 0 0 1 2 1 0 0 1 0 0 0 – 0 0 0 0 0 1 1 2
Peroxisomes 3 0 0 1 0 0 1 0 0 1 0 1 0 0 0 – 1 1 1 0 0 12 6 3
SpindlePole 3 0 3 1 3 7 4 10 7 15 10 10 0 0 1 – 0 1 13 7 13 9 3 1
Vac/Vac Membrane 27 9 21 18 53 42 40 35 36 26 3 16 1 2 1 – 2 2 134 42 107 99 513 419
Unique Cell Count 422 456 842 537 727 679 750 903 725 874 473 551 472 490 493 399 312 623 446 759 308 851 661
Labelled Cell Count 455 469 888 567 767 802 902 1080 911 1108 603 694 480 499 498 437 355 623 446 759 308 851 661


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.9 3.7 3.4 3.4 2.7 2.8 3.0 2.6 2.3 2.4 2.4 4.9 5.1 5.1 4.5 5.0 5.4 19.5 24.6 30.3
Std Deviation (1e-4) 3.0 0.7 0.8 1.0 1.3 0.9 1.2 1.0 0.8 0.6 0.8 0.9 0.9 1.0 1.0 1.5 1.6 2.2 7.1 7.9 11.4
Intensity Change (Log2) – – – -0.14 -0.12 -0.44 -0.43 -0.31 -0.49 -0.67 -0.6 -0.61 0.39 0.45 0.46 0.27 0.42 0.55 2.4 2.73 3.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – -2.4 -2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.849 0.161 0.6499 -0.8533 -0.6306 -0.0767 2.485 3.0185 2.9442 2.26 3.2246 3.4677 2.1834 3.0601 3.1045 2.7033 1.9722 2.8581
Actin 0.0735 0.0007 0.0109 0.0003 0.0001 0.0002 0.0023 0.0007 0.0011 0.001 0.0025 0.0003 0.0051 0.0003 0.0002 0.0002 0.0001 0.0006
Bud 0.0011 0.012 0.006 0.0005 0.0001 0.0002 0.0009 0.0062 0.0038 0.0043 0.0064 0.0022 0.0018 0.0124 0.021 0.0267 0.0027 0.0092
Bud Neck 0.0121 0.0001 0.0023 0.0037 0.0001 0.0004 0.0032 0.0024 0.0028 0.0048 0.0016 0.0015 0.0023 0.0006 0.0007 0.0001 0.0004 0.0029
Bud Periphery 0.0014 0.0261 0.008 0.0004 0.0001 0.0005 0.0032 0.013 0.0102 0.0126 0.0152 0.0041 0.0027 0.0033 0.0105 0.0067 0.0007 0.0074
Bud Site 0.0168 0.003 0.0169 0.0053 0 0.0002 0.002 0.0173 0.0073 0.0107 0.0017 0.0005 0.0075 0.0195 0.0119 0.0011 0.0003 0.0014
Cell Periphery 0.0006 0.0014 0.0003 0.0002 0.0001 0.0002 0.0023 0.0017 0.0012 0.0014 0.0007 0.0009 0.0008 0.0011 0.0015 0.0002 0.0001 0.0009
Cytoplasm 0.0366 0.0178 0.0318 0.0183 0.0351 0.0389 0.086 0.1095 0.0807 0.0606 0.0365 0.072 0.0467 0.0411 0.0326 0.0233 0.0504 0.079
Cytoplasmic Foci 0.065 0.0021 0.0407 0.023 0.0054 0.0071 0.0296 0.0195 0.0348 0.0092 0.0224 0.0314 0.0225 0.0091 0.0168 0.006 0.0278 0.0164
Eisosomes 0.0016 0.0006 0.0004 0.0002 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0088 0.0007 0.0009 0.0023 0.0018 0.0011 0.0076 0.0042 0.0035 0.007 0.0014 0.0061 0.0018 0.001 0.0015 0.0009 0.0005 0.0013
Endosome 0.0561 0.0002 0.0237 0.153 0.0025 0.0264 0.0705 0.0215 0.0696 0.0087 0.0439 0.0487 0.0302 0.008 0.0207 0.0296 0.0266 0.0214
Golgi 0.0161 0.0001 0.0129 0.0019 0.0001 0.0021 0.0033 0.0009 0.0038 0.0004 0.0058 0.0016 0.001 0.0002 0.0006 0.0004 0.0003 0.0006
Lipid Particles 0.0143 0.0004 0.0031 0.0021 0.001 0.0012 0.0152 0.003 0.005 0.0008 0.0064 0.0069 0.0091 0.0012 0.0028 0.0005 0.001 0.0024
Mitochondria 0.006 0.0007 0.0045 0.0018 0.0002 0.0007 0.0028 0.0016 0.0078 0.002 0.0036 0.0025 0.0013 0.0019 0.003 0.001 0.0013 0.0035
None 0.6522 0.9316 0.7952 0.6977 0.9467 0.8723 0.6944 0.7111 0.6986 0.8113 0.8166 0.7343 0.7848 0.8391 0.8067 0.8497 0.8426 0.7803
Nuclear Periphery 0.0048 0.0005 0.0048 0.004 0.0011 0.0009 0.0038 0.0015 0.0044 0.0034 0.0021 0.0017 0.0013 0.0009 0.0038 0.0018 0.0007 0.0021
Nucleolus 0.001 0.0002 0.0008 0.0031 0.0002 0.0003 0.0037 0.0042 0.0041 0.004 0.0021 0.001 0.0027 0.0021 0.004 0.0006 0.0032 0.002
Nucleus 0.002 0.0006 0.018 0.0035 0.0006 0.0011 0.0038 0.0072 0.0049 0.007 0.0024 0.0029 0.0033 0.0039 0.0064 0.0033 0.0053 0.005
Peroxisomes 0.0142 0.0001 0.0156 0.0006 0.0001 0.0004 0.0039 0.0006 0.0019 0.0008 0.0017 0.0022 0.0042 0.0001 0.0008 0.0003 0.0002 0.0021
Punctate Nuclear 0.0127 0.0002 0.0018 0.0129 0.0004 0.0008 0.0018 0.0084 0.0046 0.0067 0.0014 0.0014 0.0016 0.0057 0.0029 0.0045 0.0069 0.0042
Vacuole 0.0022 0.0009 0.0012 0.0556 0.0037 0.0382 0.0545 0.0609 0.0447 0.0363 0.0221 0.0703 0.062 0.0424 0.0457 0.0355 0.0262 0.0513
Vacuole Periphery 0.0011 0.0001 0.0004 0.0097 0.0005 0.0067 0.0052 0.0045 0.0049 0.0067 0.0034 0.0074 0.0071 0.0056 0.0056 0.0075 0.0027 0.006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.6603 2.5637 3.8047 6.6989 5.5774 6.4491 4.0502 4.8112 4.9086 7.3185
Translational Efficiency 0.8626 1.1133 0.6668 1.1603 1.4769 0.9375 1.4261 1.2083 1.3055 1.2189

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
213 1322 453 1060 1846 1084 304 808 2059 2406 757 1868

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 807.02 717.86 834.36 756.45 615.67 718.34 836.10 760.31 635.46 718.08 835.06 758.12
Standard Deviation 106.13 113.27 99.51 115.72 78.93 95.85 93.07 106.72 100.73 105.77 96.98 111.93
Intensity Change Log 2 — -0.168902 0.048066 -0.093360 — 0.222510 0.441518 0.304430 — 0.013635 0.231624 0.092371

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000230 0.000240 0.000162 0.000492 0.000229 0.000418 0.000195 0.000320 0.000229 0.000320 0.000175 0.000417
Bud Neck 0.003999 0.023558 0.001290 0.008399 0.038762 0.050177 0.002512 0.010156 0.035166 0.035551 0.001781 0.009159
Bud Site 0.019944 0.013083 0.000848 0.040567 0.006421 0.018419 0.003322 0.038636 0.007820 0.015487 0.001841 0.039732
Cell Periphery 0.000295 0.000431 0.000160 0.000447 0.000207 0.000499 0.000124 0.000414 0.000216 0.000462 0.000146 0.000433
Cytoplasm 0.334576* 0.474502* 0.236144* 0.378159* 0.385776* 0.257357* 0.217480* 0.415074* 0.380480* 0.376669* 0.228649* 0.394127*
Cytoplasmic Foci 0.038671 0.165543 0.022263 0.018090 0.288167* 0.223685* 0.009614 0.031575 0.262357* 0.191738* 0.017183 0.023923
Eisosomes 0.000075 0.000096 0.000068 0.000071 0.000424 0.000259 0.000060 0.000074 0.000388 0.000170 0.000065 0.000073
Endoplasmic Reticulum 0.002976 0.001063 0.002808 0.002191 0.001395 0.001751 0.003167 0.001865 0.001558 0.001373 0.002952 0.002050
Endosome 0.009087 0.020039 0.003816 0.010147 0.022318 0.061784 0.001999 0.007529 0.020949 0.038847 0.003086 0.009014
Golgi 0.000729 0.002307 0.000072 0.002934 0.006942 0.008061 0.000051 0.002645 0.006300 0.004899 0.000064 0.002809
Lipid Particles 0.001809 0.005769 0.002686 0.001611 0.017608 0.015521 0.001987 0.001830 0.015974 0.010162 0.002406 0.001705
Mitochondria 0.010030 0.005707 0.000294 0.008540 0.013213 0.032027 0.000410 0.004329 0.012884 0.017565 0.000341 0.006719
Mitotic Spindle 0.017336 0.002471 0.000342 0.036820 0.000890 0.004945 0.000680 0.013393 0.002591 0.003586 0.000477 0.026687
None 0.001976 0.006044 0.006880 0.009269 0.004688 0.003433 0.005224 0.006820 0.004408 0.004868 0.006215 0.008210
Nuclear Periphery 0.000964 0.000611 0.001404 0.001746 0.000404 0.000869 0.002015 0.001589 0.000462 0.000727 0.001650 0.001678
Nuclear Periphery Foci 0.001016 0.000535 0.001227 0.001243 0.000208 0.000376 0.000705 0.000565 0.000291 0.000463 0.001017 0.000950
Nucleolus 0.000387 0.002328 0.001548 0.001529 0.001455 0.003209 0.001157 0.000813 0.001344 0.002725 0.001391 0.001219
Nucleus 0.337144* 0.155169* 0.662090* 0.389954* 0.138432* 0.104159 0.687052* 0.372320* 0.158988* 0.132187* 0.672114* 0.382327*
Peroxisomes 0.000731 0.005973 0.000409 0.002888 0.013297 0.021185 0.000242 0.001617 0.011997 0.012827 0.000342 0.002338
Vacuole 0.216642* 0.111297 0.055262 0.081384 0.057363 0.184933 0.061804 0.084718 0.073840 0.144473 0.057889 0.082826
Vacuole Periphery 0.001385 0.003235 0.000227 0.003520 0.001800 0.006935 0.000200 0.003717 0.001757 0.004902 0.000216 0.003605

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.19 0.07 -3.39 -2.49 -3.70 -1.70 -1.59 -0.93 1.21 0.62 -1.33 -0.71 -2.89 -0.74 -2.66
Bud Neck -9.43 5.03 -4.31 6.84 -8.60 -2.97 17.27 12.89 12.05 -3.18 -0.03 18.32 13.75 13.64 -8.07
Bud Site 0.28 2.82 -2.00 -3.47 -7.43 -5.07 1.56 -5.06 -1.69 -5.41 -5.20 5.26 -7.13 -3.67 -9.20
Cell Periphery -1.21 0.75 -1.31 0.78 -2.49 -3.53 4.98 -1.30 1.93 -2.41 -2.38 4.13 -2.07 1.53 -3.42
Cytoplasm -7.77 3.96 -0.37 12.80 -6.85 13.13 17.11 2.65 -7.65 -12.34 0.35 17.94 4.92 4.45 -12.89
Cytoplasmic Foci -20.61 1.39 3.38 28.33 2.78 7.54 52.40 46.70 28.51 -9.38 10.54 46.39 46.89 39.88 -2.05
Eisosomes -2.02 0.47 1.07 4.59 0.62 3.88 8.41 8.54 11.48 0.31 5.98 8.54 8.66 12.10 0.63
Endoplasmic Reticulum 1.73 -1.26 -0.84 -9.95 1.17 -0.89 -7.81 -4.10 -2.71 5.19 0.50 -8.40 -5.55 -7.90 4.62
Endosome -7.13 1.10 -0.10 7.54 -1.37 -11.17 15.08 10.70 16.57 -2.99 -8.57 13.52 10.96 17.48 -2.55
Golgi -3.73 1.11 -1.71 1.35 -2.56 -1.02 12.09 4.86 4.99 -1.52 2.04 12.08 6.69 4.99 -2.72
Lipid Particles -7.89 -3.71 0.76 8.68 4.87 1.31 15.69 16.61 9.92 1.43 5.38 15.28 16.79 12.46 4.76
Mitochondria -0.20 1.01 0.06 0.83 -3.52 -5.64 11.59 7.83 9.08 -3.00 -2.74 11.03 6.57 8.20 -4.50
Mitotic Spindle 1.37 1.74 -2.26 -6.11 -6.70 -3.04 -1.44 -4.21 -2.13 -0.60 -1.80 -0.70 -7.00 -6.37 -5.33
None -2.70 -1.67 -4.60 -0.59 -0.46 1.19 0.65 -0.58 -1.51 -1.10 -0.34 -0.50 -1.88 -1.45 -0.82
Nuclear Periphery 7.09 -1.86 -7.05 -12.90 -5.92 -3.87 -15.34 -8.57 -5.88 2.16 -3.32 -18.56 -15.33 -12.49 -2.09
Nuclear Periphery Foci 1.24 -2.10 -1.05 -4.81 1.66 -2.07 -5.27 -5.77 -3.16 1.88 -3.31 -7.82 -8.25 -5.77 2.46
Nucleolus -5.00 -2.76 -3.18 2.51 0.52 -2.84 2.23 4.49 4.10 0.61 -3.79 0.52 1.85 4.66 0.76
Nucleus 8.47 -13.19 -4.00 -25.04 14.38 5.47 -34.54 -24.45 -26.24 14.58 4.63 -47.58 -32.02 -36.08 20.37
Peroxisomes -10.42 0.63 -1.63 5.16 -1.78 -3.95 13.58 11.51 10.98 -1.68 -0.76 13.42 10.97 11.90 -2.34
Vacuole 7.62 9.53 5.86 -4.11 -8.46 -15.97 -4.92 -11.49 4.67 -2.28 -11.89 -1.02 -11.23 0.34 -7.76
Vacuole Periphery -3.60 1.45 -1.71 1.97 -3.31 -6.72 9.98 -0.38 3.62 -1.91 -6.55 10.13 -0.45 4.16 -3.28
Standard name
Human Ortholog
Description Membrane protein localized to the shmoo tip; required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Fus1

Fus1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fus1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available