Standard name
Human Ortholog
Description Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase; phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.07 0 0 0 0 0 0.08 – 0.09 0.14 0.09 0.16 0 0 0 0.2 0.13 0.2 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.99 0.99 0.99 0.93 – 0.89 0.82 0.87 0.78 0.99 0.99 1.0 0.91 0.94 0.91 0.96 0.93 0.97 0.88 0.89 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0.05 0.1 0.08 0.12 0 0 0 0 0.06 0.07 0 0 0 0 0 0.06
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 2 2 0 1 1 1 – 0 1 5 2 0 1 0 0 1 0 0 0 1 0 2 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 14 2 9 4 4 5 31 – 25 46 20 40 1 3 0 114 50 89 0 0 0 1 0 0
Cytoplasm 207 268 306 161 268 279 346 – 249 266 196 201 193 237 255 532 351 406 215 292 367 141 235 228
Endoplasmic Reticulum 0 0 1 0 2 2 8 – 15 34 17 30 0 0 0 25 21 32 2 0 0 5 10 17
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 1 0 0 0 0 – 2 0 0 0 0 0 0 1 1 0 0 0 0 0 1 6
Mitochondria 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleus 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 4 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 0 0 0 – 0 2 0 1 0 0 0 1 0 0 0 7 1 1 4 5
Unique Cell Count 212 271 312 162 270 283 372 281 324 226 257 194 240 255 583 373 448 225 316 380 161 264 268
Labelled Cell Count 223 272 320 165 275 287 386 291 351 240 276 195 241 255 673 424 527 225 316 380 161 264 268


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 428.5 320.4 299.1 294.5 269.1 217.6 184.0 – 135.0 110.4 104.4 103.1 359.1 345.3 330.7 498.2 473.0 507.1 324.9 331.6 325.9
Std Deviation (1e-4) 76.3 77.5 73.4 76.3 60.5 51.3 44.9 – 37.7 35.0 37.5 32.9 76.8 70.8 73.6 121.9 112.5 103.9 57.5 69.0 72.4
Intensity Change (Log2) – – – -0.02 -0.15 -0.46 -0.7 – -1.15 -1.44 -1.52 -1.54 0.26 0.21 0.14 0.74 0.66 0.76 0.12 0.15 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -1.1 -0.9 3.0 – 3.1 5.1 3.0 5.4 0 -1.3 0 6.9 4.9 6.9
Cytoplasm 1.1 1.2 0.5 -3.1 – -4.7 -6.7 -5.2 -7.6 1.3 0.6 2.2 -4.0 -2.6 -4.2
Endoplasmic Reticulum 0 0 0 0 – 3.8 5.6 4.6 5.9 0 0 0 3.4 3.9 4.5
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 455.1469 611.9749 469.8879 389.9071 490.1023 527.7218 553.1044 727.1972 593.3145 413.3512 537.3315 628.2797 470.9727 704.0134 548.9541 488.3047 635.5526 581.5817
Actin 0.013 0.0004 0.0144 0.0015 0.0011 0.0017 0.0008 0.0002 0.0056 0.0004 0.0005 0.001 0.0107 0.0005 0.0049 0.0006 0.0024 0.0024
Bud 0.0031 0.0014 0.0024 0.013 0.0029 0.0033 0.0016 0.0016 0.0017 0.0094 0.0012 0.0014 0.0033 0.002 0.0026 0.007 0.0027 0.004
Bud Neck 0.0016 0.0008 0.0016 0.0051 0.0014 0.004 0.0007 0.0004 0.0014 0.001 0.0012 0.0022 0.0006 0.0003 0.0006 0.0006 0.0009 0.0013
Bud Periphery 0.0017 0.0004 0.0024 0.0104 0.0005 0.002 0.0013 0.0005 0.001 0.0043 0.0005 0.0005 0.0019 0.0006 0.0015 0.003 0.0008 0.0045
Bud Site 0.0021 0.0007 0.0019 0.0061 0.0003 0.0004 0.0009 0.0007 0.0037 0.0004 0.0003 0.0002 0.0014 0.0013 0.0021 0.0005 0.0002 0.0004
Cell Periphery 0.0277 0.0078 0.0129 0.0484 0.0049 0.0114 0.0274 0.0094 0.0182 0.005 0.0132 0.0039 0.0119 0.008 0.0041 0.0029 0.001 0.0139
Cytoplasm 0.6568 0.9167 0.7717 0.4347 0.7995 0.7557 0.801 0.9508 0.8469 0.7136 0.6941 0.8922 0.6986 0.9213 0.8116 0.6977 0.9104 0.7836
Cytoplasmic Foci 0.0148 0.0003 0.0113 0.0045 0.0473 0.0011 0.0076 0.0002 0.0084 0.0224 0.0292 0.0025 0.0134 0.0008 0.016 0.0071 0.0009 0.0017
Eisosomes 0.0016 0 0.0006 0.0007 0.0005 0.0001 0.0011 0 0.0004 0.001 0.002 0.0002 0.0022 0 0.0005 0.0002 0.0001 0.0013
Endoplasmic Reticulum 0.0098 0.0009 0.0021 0.0007 0.0004 0.0015 0.0061 0.0006 0.0013 0.0078 0.0017 0.0023 0.0038 0.0002 0.0026 0.0003 0.0011 0.0036
Endosome 0.01 0.0002 0.0097 0.0008 0.0011 0.001 0.0005 0.0001 0.0007 0.0382 0.0166 0.0007 0.0048 0.0001 0.0139 0.0003 0.0007 0.0017
Golgi 0.0034 0 0.0055 0.0002 0.0004 0.0002 0.0001 0 0.0005 0.002 0.0017 0.0002 0.0006 0 0.007 0.0001 0.0001 0.0008
Lipid Particles 0.005 0.0001 0.0024 0.0031 0.0134 0.0004 0.0021 0.0002 0.0012 0.0034 0.0073 0.0009 0.0026 0.0002 0.005 0.0009 0.0001 0.0005
Mitochondria 0.0223 0.0045 0.0289 0.0285 0.019 0.0173 0.0116 0.0009 0.0095 0.0215 0.0152 0.0069 0.0336 0.0022 0.0215 0.0443 0.0044 0.0221
None 0.0127 0.0011 0.0079 0.0106 0.0122 0.002 0.0087 0.0006 0.012 0.0119 0.0316 0.0101 0.0288 0.0021 0.0104 0.0082 0.0032 0.0023
Nuclear Periphery 0.0435 0.0071 0.0178 0.0328 0.0092 0.0311 0.0361 0.0044 0.0135 0.0084 0.024 0.018 0.0418 0.0043 0.0101 0.0137 0.0212 0.0322
Nucleolus 0.0437 0.0086 0.0302 0.1107 0.0157 0.0123 0.0183 0.0008 0.0201 0.0698 0.0569 0.0031 0.0345 0.0025 0.0269 0.0806 0.0011 0.0057
Nucleus 0.0705 0.0283 0.0333 0.0875 0.0405 0.0802 0.0344 0.0128 0.0225 0.036 0.0479 0.0314 0.073 0.04 0.031 0.0974 0.0335 0.0567
Peroxisomes 0.0034 0.0001 0.0067 0.0013 0.0032 0.0004 0.0007 0 0.0012 0.0012 0.0009 0.0004 0.0013 0.0001 0.0079 0.0011 0.0001 0.0004
Punctate Nuclear 0.0028 0.0001 0.001 0.0004 0.0013 0.0002 0.0011 0 0.0004 0.0007 0.0019 0.0008 0.0015 0.0001 0.0015 0.0002 0.0004 0.0005
Vacuole 0.0367 0.0169 0.0228 0.1086 0.0122 0.0441 0.0301 0.0132 0.0244 0.0301 0.0348 0.0155 0.0174 0.0111 0.0102 0.0172 0.0082 0.0336
Vacuole Periphery 0.014 0.0035 0.0126 0.0902 0.0129 0.0297 0.0077 0.0024 0.0054 0.0115 0.0172 0.0056 0.0124 0.0023 0.0081 0.0161 0.0065 0.0268

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 125.972 115.4882 117.7193 121.6628 132.9277 219.1802 182.7388 167.7207 192.666 156.6044
Translational Efficiency 1.3275 1.3366 1.28 1.5763 1.3071 1.1785 1.2336 1.3395 1.2085 1.1963

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase; phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

His4

His4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (His4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available