Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in membrane-trafficking events at plasma membrane; interacts with actin regulators Sla1p and Las17p, ubiquitin, Arf3p to couple actin dynamics to membrane trafficking processes; similar structure to GGA family of proteins with N-terminal VHS domain and GAT domain; binds Las17p, which is homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly, actin polymerization; may mediate disassembly of Pan1 complex from endocytic coat |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 3 |
Bud | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 2 | 3 | 4 | 5 | 6 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 4 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 4 | 3 | 2 | 3 | 4 | 4 | 8 | 5 | 2 | 9 | 0 | 0 | 0 | 10 | 9 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 437 | 368 | 228 | 252 | 348 | 293 | 364 | 402 | 275 | 331 | 194 | 211 | 122 | 226 | 277 | 195 | 298 | 201 | 429 | 343 | 250 | 172 | 337 | 319 |
Endoplasmic Reticulum | 1 | 5 | 3 | 1 | 1 | 1 | 2 | 7 | 9 | 9 | 6 | 12 | 0 | 0 | 3 | 5 | 6 | 6 | 0 | 0 | 2 | 1 | 2 | 0 |
Endosome | 2 | 1 | 0 | 1 | 2 | 0 | 4 | 3 | 2 | 4 | 1 | 0 | 1 | 1 | 4 | 1 | 3 | 7 | 4 | 3 | 1 | 1 | 1 | 3 |
Golgi | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 3 | 2 | 0 | 0 | 1 | 0 |
Mitochondria | 5 | 2 | 0 | 1 | 7 | 20 | 53 | 42 | 45 | 43 | 49 | 34 | 0 | 0 | 0 | 0 | 5 | 2 | 3 | 1 | 2 | 0 | 1 | 0 |
Nucleus | 4 | 5 | 3 | 9 | 7 | 14 | 23 | 23 | 32 | 26 | 12 | 31 | 1 | 6 | 2 | 8 | 9 | 3 | 1 | 9 | 3 | 1 | 2 | 1 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 4 | 8 |
Vac/Vac Membrane | 2 | 4 | 7 | 38 | 97 | 68 | 83 | 131 | 46 | 124 | 19 | 114 | 10 | 4 | 22 | 42 | 51 | 33 | 3 | 3 | 1 | 1 | 2 | 3 |
Unique Cell Count | 446 | 383 | 235 | 282 | 435 | 366 | 494 | 579 | 382 | 503 | 268 | 378 | 127 | 233 | 293 | 237 | 343 | 235 | 453 | 379 | 268 | 184 | 364 | 356 |
Labelled Cell Count | 452 | 390 | 249 | 305 | 464 | 399 | 537 | 617 | 420 | 548 | 291 | 419 | 134 | 238 | 310 | 264 | 387 | 255 | 453 | 379 | 268 | 184 | 364 | 356 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 9.3 | 7.7 | 8.0 | 8.5 | 7.7 | 7.7 | 8.4 | 7.9 | 8.0 | 7.5 | 8.3 | 8.6 | 8.8 | 9.0 | 10.0 | 10.4 | 10.0 | 8.3 | 8.9 | 9.3 |
Std Deviation (1e-4) | 1.0 | 1.5 | 1.1 | 1.2 | 1.3 | 1.2 | 1.4 | 1.3 | 1.5 | 1.4 | 1.4 | 1.5 | 1.3 | 1.3 | 1.5 | 1.9 | 1.8 | 1.7 | 1.5 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.14 | -0.01 | -0.0 | 0.11 | 0.02 | 0.05 | -0.05 | 0.09 | 0.16 | 0.18 | 0.22 | 0.37 | 0.43 | 0.36 | 0.1 | 0.2 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 0.7 | 0 |
Cytoplasm | -3.4 | -6.1 | -6.0 | -7.5 | -8.5 | -7.8 | -9.2 | -7.5 | -11.0 | -0.5 | 0 | -1.4 | -5.3 | -4.2 | -4.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.6 | 5.2 | 4.2 | 5.5 | 4.6 | 6.9 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.4 | 0 | 1.8 | 2.3 | 2.0 | 3.7 | 2.5 | 2.1 | 3.6 | 0 | 0 | 0 | 1.5 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 4.2 | 6.6 | 5.6 | 5.3 | 6.8 | 3.9 | 7.2 | 2.1 | 8.2 | 2.1 | -0.9 | 2.3 | 5.3 | 4.7 | 4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.8041 | 5.9685 | 5.1563 | 5.0467 | 5.203 | 5.9255 | 5.007 | 5.2034 | 5.6207 | 5.5192 | 5.5831 | 5.9889 | 4.4149 | 5.4305 | 5.1822 | 4.7381 | 4.9801 | 5.2871 |
Actin | 0.0623 | 0.0004 | 0.0113 | 0.0006 | 0.0375 | 0.0002 | 0.0032 | 0.0015 | 0.0026 | 0.0002 | 0.0008 | 0.0009 | 0.0222 | 0.0007 | 0.0005 | 0.0315 | 0.006 | 0.0029 |
Bud | 0.0012 | 0.0001 | 0.001 | 0.0004 | 0.0009 | 0.0001 | 0.0025 | 0.0007 | 0.0009 | 0.0002 | 0.0002 | 0.0006 | 0.0009 | 0.0003 | 0.0002 | 0.0022 | 0.0019 | 0.0001 |
Bud Neck | 0.007 | 0.0002 | 0.0015 | 0.0009 | 0.0008 | 0.0011 | 0.0008 | 0.0009 | 0.0009 | 0.0018 | 0.002 | 0.0048 | 0.0018 | 0.0005 | 0.0006 | 0.0034 | 0.0013 | 0.0018 |
Bud Periphery | 0.0014 | 0 | 0.0019 | 0.0002 | 0.0028 | 0 | 0.0013 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0012 | 0.0001 | 0.0001 | 0.0024 | 0.0039 | 0 |
Bud Site | 0.0088 | 0.0004 | 0.0106 | 0.0008 | 0.0018 | 0 | 0.0017 | 0.0059 | 0.0012 | 0.0004 | 0.0003 | 0.0002 | 0.0086 | 0.0028 | 0.0005 | 0.0028 | 0.0037 | 0.0002 |
Cell Periphery | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0006 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.7098 | 0.9598 | 0.8066 | 0.8608 | 0.681 | 0.9019 | 0.8457 | 0.8157 | 0.7944 | 0.7701 | 0.7954 | 0.8167 | 0.6841 | 0.9224 | 0.9151 | 0.7138 | 0.7756 | 0.9147 |
Cytoplasmic Foci | 0.0352 | 0.0021 | 0.0295 | 0.0045 | 0.0174 | 0.0024 | 0.0122 | 0.0097 | 0.0144 | 0.0085 | 0.0076 | 0.0075 | 0.0318 | 0.005 | 0.0103 | 0.0246 | 0.0165 | 0.007 |
Eisosomes | 0.0005 | 0 | 0.0003 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.0002 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0104 | 0.0016 | 0.0034 | 0.0043 | 0.0071 | 0.003 | 0.0052 | 0.0029 | 0.0023 | 0.0019 | 0.0049 | 0.0029 | 0.0129 | 0.0031 | 0.0024 | 0.0035 | 0.0051 | 0.0032 |
Endosome | 0.0428 | 0.0006 | 0.0147 | 0.0353 | 0.038 | 0.0017 | 0.0065 | 0.0057 | 0.0054 | 0.0042 | 0.0059 | 0.0032 | 0.0686 | 0.0032 | 0.0087 | 0.036 | 0.0404 | 0.0036 |
Golgi | 0.009 | 0.0001 | 0.009 | 0.0029 | 0.0218 | 0 | 0.0006 | 0.0003 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.018 | 0.0004 | 0.0015 | 0.0195 | 0.013 | 0.0003 |
Lipid Particles | 0.0117 | 0 | 0.0076 | 0.0071 | 0.0279 | 0 | 0.0013 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0318 | 0.0017 | 0.0006 | 0.0051 | 0.0169 | 0.0001 |
Mitochondria | 0.0047 | 0.0001 | 0.0117 | 0.0026 | 0.0455 | 0.0001 | 0.0008 | 0.0005 | 0.0005 | 0.0003 | 0.0005 | 0.0003 | 0.0118 | 0.0006 | 0.0055 | 0.0768 | 0.0168 | 0.0003 |
None | 0.0168 | 0.0074 | 0.0241 | 0.0276 | 0.0114 | 0.0056 | 0.0254 | 0.0528 | 0.0172 | 0.0128 | 0.006 | 0.0135 | 0.0154 | 0.0109 | 0.0056 | 0.004 | 0.0037 | 0.0046 |
Nuclear Periphery | 0.0188 | 0.0019 | 0.0042 | 0.005 | 0.0359 | 0.0041 | 0.0077 | 0.0057 | 0.0047 | 0.0049 | 0.0114 | 0.0055 | 0.0162 | 0.0035 | 0.0029 | 0.0031 | 0.01 | 0.0044 |
Nucleolus | 0.0005 | 0 | 0.0005 | 0.0004 | 0.0008 | 0 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0031 | 0.0002 | 0.0001 | 0.0002 | 0.0009 | 0.0001 |
Nucleus | 0.0293 | 0.0237 | 0.0409 | 0.0358 | 0.0169 | 0.0779 | 0.0726 | 0.0866 | 0.1443 | 0.1766 | 0.1501 | 0.1293 | 0.0314 | 0.039 | 0.0411 | 0.0462 | 0.0698 | 0.0477 |
Peroxisomes | 0.016 | 0.0001 | 0.0141 | 0.0006 | 0.0378 | 0 | 0.0014 | 0.0003 | 0.0014 | 0.0002 | 0.0005 | 0.0003 | 0.0207 | 0.0012 | 0.0006 | 0.0176 | 0.0053 | 0.0025 |
Punctate Nuclear | 0.0085 | 0.0012 | 0.0048 | 0.0017 | 0.0051 | 0.0012 | 0.009 | 0.007 | 0.0062 | 0.0161 | 0.0122 | 0.0122 | 0.0077 | 0.003 | 0.0028 | 0.0025 | 0.0065 | 0.0053 |
Vacuole | 0.0034 | 0.0003 | 0.0014 | 0.0065 | 0.0037 | 0.0005 | 0.0015 | 0.0025 | 0.0013 | 0.001 | 0.0013 | 0.001 | 0.0077 | 0.0011 | 0.0009 | 0.0027 | 0.0016 | 0.0007 |
Vacuole Periphery | 0.0015 | 0 | 0.0004 | 0.0019 | 0.0054 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0032 | 0.0002 | 0.0002 | 0.0018 | 0.001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.2621 | 58.349 | 46.0465 | 37.7908 | 30.2242 | 38.313 | 57.1237 | 49.2994 | 53.4473 | 41.9175 |
Translational Efficiency | 1.2452 | 1.0498 | 0.8973 | 1.419 | 1.6051 | 1.3377 | 1.1514 | 1.3467 | 1.1396 | 1.0714 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2244 | 198 | 2772 | 101 | 1331 | 2041 | 1200 | 752 | 3575 | 2239 | 3972 | 853 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 892.70 | 1005.25 | 998.68 | 1148.96 | 848.66 | 937.04 | 1150.21 | 1091.29 | 876.30 | 943.07 | 1044.46 | 1098.12 |
Standard Deviation | 117.72 | 152.79 | 148.85 | 228.25 | 117.84 | 131.14 | 143.34 | 161.87 | 119.67 | 134.60 | 162.82 | 172.09 |
Intensity Change Log 2 | — | 0.171307 | 0.161847 | 0.364081 | — | 0.142924 | 0.438639 | 0.362776 | — | 0.157544 | 0.303377 | 0.363445 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000111 | 0.004093 | 0.001042 | 0.025930 | 0.000874 | 0.000342 | 0.000590 | 0.018150 | 0.000395 | 0.000674 | 0.000905 | 0.019071 |
Bud Neck | 0.002456 | 0.004526 | 0.006191 | 0.004389 | 0.001542 | 0.005070 | 0.002466 | 0.012197 | 0.002115 | 0.005022 | 0.005066 | 0.011272 |
Bud Site | 0.002538 | 0.024550 | 0.016451 | 0.127451* | 0.003021 | 0.009802 | 0.004803 | 0.110450* | 0.002718 | 0.011106 | 0.012932 | 0.112463* |
Cell Periphery | 0.000054 | 0.000129 | 0.000105 | 0.000237 | 0.000136 | 0.000072 | 0.000144 | 0.000224 | 0.000084 | 0.000077 | 0.000116 | 0.000225 |
Cytoplasm | 0.724314* | 0.618334* | 0.594077* | 0.133706* | 0.690887* | 0.714117* | 0.426813* | 0.200291* | 0.711869* | 0.705647* | 0.543544* | 0.192407* |
Cytoplasmic Foci | 0.040422 | 0.112297 | 0.004082 | 0.117035 | 0.085020 | 0.052807 | 0.006582 | 0.087001 | 0.057027 | 0.058068 | 0.004837 | 0.090558 |
Eisosomes | 0.000016 | 0.000048 | 0.000012 | 0.000052 | 0.000045 | 0.000022 | 0.000029 | 0.000095 | 0.000027 | 0.000024 | 0.000017 | 0.000090 |
Endoplasmic Reticulum | 0.000484 | 0.001724 | 0.001113 | 0.001385 | 0.001566 | 0.000740 | 0.003206 | 0.002606 | 0.000887 | 0.000827 | 0.001745 | 0.002462 |
Endosome | 0.001303 | 0.008741 | 0.001055 | 0.025993 | 0.004329 | 0.004842 | 0.001300 | 0.066082 | 0.002429 | 0.005186 | 0.001129 | 0.061336 |
Golgi | 0.000399 | 0.004906 | 0.000738 | 0.005913 | 0.000643 | 0.002702 | 0.000231 | 0.022394 | 0.000490 | 0.002897 | 0.000584 | 0.020442 |
Lipid Particles | 0.000486 | 0.002165 | 0.000419 | 0.009262 | 0.001799 | 0.001185 | 0.000962 | 0.003319 | 0.000975 | 0.001272 | 0.000583 | 0.004023 |
Mitochondria | 0.000489 | 0.000334 | 0.001643 | 0.000664 | 0.000657 | 0.001120 | 0.000506 | 0.025374 | 0.000552 | 0.001051 | 0.001300 | 0.022448 |
Mitotic Spindle | 0.001215 | 0.013729 | 0.030864 | 0.105009* | 0.002286 | 0.009648 | 0.005990 | 0.177409* | 0.001614 | 0.010009 | 0.023349 | 0.168837* |
None | 0.005709 | 0.006133 | 0.007775 | 0.001788 | 0.005519 | 0.005830 | 0.008219 | 0.002100 | 0.005638 | 0.005857 | 0.007909 | 0.002063 |
Nuclear Periphery | 0.000193 | 0.000375 | 0.001581 | 0.000439 | 0.000273 | 0.000390 | 0.001325 | 0.001904 | 0.000223 | 0.000388 | 0.001504 | 0.001731 |
Nuclear Periphery Foci | 0.000624 | 0.002973 | 0.000468 | 0.002678 | 0.002695 | 0.001384 | 0.002500 | 0.006783 | 0.001395 | 0.001525 | 0.001082 | 0.006297 |
Nucleolus | 0.000341 | 0.000305 | 0.000701 | 0.000097 | 0.000340 | 0.000633 | 0.000226 | 0.001549 | 0.000341 | 0.000604 | 0.000557 | 0.001377 |
Nucleus | 0.185307* | 0.103392 | 0.287957* | 0.033670 | 0.150307* | 0.123543 | 0.456115* | 0.034448 | 0.172277* | 0.121761 | 0.338760* | 0.034356 |
Peroxisomes | 0.000444 | 0.015716 | 0.000979 | 0.146579* | 0.001072 | 0.002689 | 0.001860 | 0.055127 | 0.000678 | 0.003841 | 0.001245 | 0.065956 |
Vacuole | 0.032927 | 0.075264 | 0.041213 | 0.228867* | 0.046271 | 0.062281 | 0.076051 | 0.155227 | 0.037895 | 0.063429 | 0.051738 | 0.163946 |
Vacuole Periphery | 0.000170 | 0.000263 | 0.001535 | 0.028856 | 0.000719 | 0.000781 | 0.000081 | 0.017270 | 0.000374 | 0.000736 | 0.001096 | 0.018642 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.72 | -2.64 | -2.23 | -1.16 | -2.11 | 0.96 | 0.27 | -3.70 | -4.14 | -3.93 | -1.04 | -1.13 | -4.57 | -4.38 | -4.42 |
Bud Neck | -2.59 | -9.45 | -3.32 | -1.18 | 0.58 | -5.92 | -7.38 | -11.68 | -4.59 | -7.31 | -5.39 | -11.42 | -11.36 | -4.67 | -3.85 |
Bud Site | -4.58 | -7.24 | -3.98 | -1.94 | -3.27 | -5.97 | -3.42 | -11.13 | -9.53 | -10.64 | -7.71 | -7.79 | -11.91 | -9.72 | -10.15 |
Cell Periphery | -4.96 | -6.32 | -4.07 | -1.19 | -2.51 | 7.62 | -2.15 | -2.03 | -7.34 | -0.51 | 1.33 | -5.61 | -7.07 | -7.47 | -4.21 |
Cytoplasm | 6.63 | 35.93 | 23.91 | 12.46 | 12.53 | -0.62 | 40.10 | 37.63 | 39.43 | 5.49 | 3.90 | 49.53 | 48.36 | 41.70 | 18.18 |
Cytoplasmic Foci | -5.55 | 21.58 | -0.38 | 3.89 | -3.96 | 8.15 | 24.51 | 9.59 | 3.09 | -11.29 | -0.22 | 32.00 | 4.44 | 4.23 | -12.26 |
Eisosomes | -4.68 | -3.14 | -7.82 | -1.32 | -7.35 | 7.28 | 1.54 | -3.50 | -6.80 | -4.34 | 0.64 | 0.90 | -6.96 | -7.10 | -7.24 |
Endoplasmic Reticulum | -4.81 | -16.01 | -5.02 | -0.10 | -0.89 | 3.21 | -8.40 | -1.29 | -5.54 | 7.63 | 0.02 | -13.19 | -5.26 | -5.58 | 2.32 |
Endosome | -2.28 | 1.67 | -3.45 | -0.43 | -3.66 | -0.57 | 9.04 | -5.87 | -5.62 | -7.23 | -4.41 | 7.08 | -7.04 | -5.65 | -7.75 |
Golgi | -1.37 | 0.23 | -2.00 | 0.93 | -1.97 | -2.63 | 2.83 | -3.32 | -1.76 | -3.71 | -3.17 | 1.06 | -3.53 | -1.56 | -3.68 |
Lipid Particles | -4.09 | -5.15 | -2.92 | -2.17 | -2.82 | 2.31 | 2.44 | -6.10 | -7.45 | -8.88 | -1.32 | 0.80 | -8.44 | -6.82 | -8.72 |
Mitochondria | 1.66 | -4.94 | -0.28 | -1.03 | 2.81 | -1.25 | -0.13 | -2.71 | -2.52 | -2.72 | -2.23 | -3.91 | -2.78 | -2.52 | -2.41 |
Mitotic Spindle | -2.15 | -12.29 | -4.35 | -3.67 | -2.90 | -3.71 | -2.94 | -11.96 | -11.01 | -11.19 | -5.04 | -12.44 | -12.87 | -11.63 | -9.65 |
None | 0.33 | -5.03 | 4.98 | 2.01 | 9.88 | 0.10 | -1.35 | 1.27 | 1.38 | 4.33 | 0.27 | -4.33 | 2.67 | 1.74 | 7.68 |
Nuclear Periphery | -4.93 | -14.37 | -7.46 | -5.47 | 6.18 | -2.52 | -29.83 | -10.07 | -8.82 | 1.23 | -3.67 | -19.39 | -11.02 | -9.30 | 2.58 |
Nuclear Periphery Foci | -5.84 | 0.30 | -2.83 | -0.84 | -2.84 | 3.26 | -0.64 | -1.52 | -3.04 | -1.20 | -0.81 | 0.70 | -3.82 | -3.23 | -3.96 |
Nucleolus | 1.00 | -1.64 | 0.13 | -0.83 | 1.70 | -2.19 | 0.07 | -3.75 | -1.12 | -3.41 | -2.05 | -1.60 | -3.63 | -1.07 | -1.85 |
Nucleus | 6.68 | -29.54 | 8.13 | 2.41 | 20.33 | 4.10 | -39.42 | 5.79 | 3.09 | 39.87 | 11.29 | -45.67 | 11.24 | 3.75 | 42.63 |
Peroxisomes | -3.40 | -0.49 | -2.90 | -1.57 | -2.89 | -2.78 | 0.53 | -6.52 | -5.89 | -6.61 | -5.18 | 0.12 | -7.26 | -6.04 | -7.26 |
Vacuole | -6.47 | -15.83 | -12.04 | -9.17 | -10.44 | -7.31 | -15.55 | -25.36 | -22.63 | -15.55 | -12.27 | -21.50 | -28.88 | -24.45 | -22.38 |
Vacuole Periphery | -1.35 | -1.79 | -1.00 | -0.98 | -0.89 | -0.01 | 2.14 | -3.10 | -3.15 | -3.88 | -1.31 | -0.57 | -3.49 | -3.15 | -3.32 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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