Standard name
Human Ortholog
Description Protein involved in membrane-trafficking events at plasma membrane; interacts with actin regulators Sla1p and Las17p, ubiquitin, Arf3p to couple actin dynamics to membrane trafficking processes; similar structure to GGA family of proteins with N-terminal VHS domain and GAT domain; binds Las17p, which is homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly, actin polymerization; may mediate disassembly of Pan1 complex from endocytic coat
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.97 0.89 0.8 0.8 0.74 0.69 0.72 0.66 0.72 0.56 0.96 0.97 0.95 0.82 0.87 0.86 0.95 0.91 0.94 0.94 0.93 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.11 0.07 0.12 0.09 0.18 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.05 0 0.08 0.05 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.13 0.22 0.19 0.17 0.23 0.12 0.25 0.07 0.3 0.08 0 0.08 0.18 0.15 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 1 1 0 2 0 2 0 0 1 0 2 0 1 1 0 1 0 3
Bud 1 1 1 0 0 0 2 2 3 4 5 6 0 0 0 0 1 0 1 3 0 0 4 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2
Bud Site 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 4 3 2 3 4 4 8 5 2 9 0 0 0 10 9 1 0 0 0 0 0 0
Cytoplasm 437 368 228 252 348 293 364 402 275 331 194 211 122 226 277 195 298 201 429 343 250 172 337 319
Endoplasmic Reticulum 1 5 3 1 1 1 2 7 9 9 6 12 0 0 3 5 6 6 0 0 2 1 2 0
Endosome 2 1 0 1 2 0 4 3 2 4 1 0 1 1 4 1 3 7 4 3 1 1 1 3
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 2 3 2 0 0 1 0
Mitochondria 5 2 0 1 7 20 53 42 45 43 49 34 0 0 0 0 5 2 3 1 2 0 1 0
Nucleus 4 5 3 9 7 14 23 23 32 26 12 31 1 6 2 8 9 3 1 9 3 1 2 1
Nuclear Periphery 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 1 2 0 4 8
Vac/Vac Membrane 2 4 7 38 97 68 83 131 46 124 19 114 10 4 22 42 51 33 3 3 1 1 2 3
Unique Cell Count 446 383 235 282 435 366 494 579 382 503 268 378 127 233 293 237 343 235 453 379 268 184 364 356
Labelled Cell Count 452 390 249 305 464 399 537 617 420 548 291 419 134 238 310 264 387 255 453 379 268 184 364 356


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 9.3 7.7 8.0 8.5 7.7 7.7 8.4 7.9 8.0 7.5 8.3 8.6 8.8 9.0 10.0 10.4 10.0 8.3 8.9 9.3
Std Deviation (1e-4) 1.0 1.5 1.1 1.2 1.3 1.2 1.4 1.3 1.5 1.4 1.4 1.5 1.3 1.3 1.5 1.9 1.8 1.7 1.5 1.4 1.5
Intensity Change (Log2) – – – 0.04 0.14 -0.01 -0.0 0.11 0.02 0.05 -0.05 0.09 0.16 0.18 0.22 0.37 0.43 0.36 0.1 0.2 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1.6 0.7 0
Cytoplasm -3.4 -6.1 -6.0 -7.5 -8.5 -7.8 -9.2 -7.5 -11.0 -0.5 0 -1.4 -5.3 -4.2 -4.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1.5 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.6 5.2 4.2 5.5 4.6 6.9 4.7 0 0 0 0 0 0
Nucleus 1.4 0 1.8 2.3 2.0 3.7 2.5 2.1 3.6 0 0 0 1.5 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 4.2 6.6 5.6 5.3 6.8 3.9 7.2 2.1 8.2 2.1 -0.9 2.3 5.3 4.7 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.8041 5.9685 5.1563 5.0467 5.203 5.9255 5.007 5.2034 5.6207 5.5192 5.5831 5.9889 4.4149 5.4305 5.1822 4.7381 4.9801 5.2871
Actin 0.0623 0.0004 0.0113 0.0006 0.0375 0.0002 0.0032 0.0015 0.0026 0.0002 0.0008 0.0009 0.0222 0.0007 0.0005 0.0315 0.006 0.0029
Bud 0.0012 0.0001 0.001 0.0004 0.0009 0.0001 0.0025 0.0007 0.0009 0.0002 0.0002 0.0006 0.0009 0.0003 0.0002 0.0022 0.0019 0.0001
Bud Neck 0.007 0.0002 0.0015 0.0009 0.0008 0.0011 0.0008 0.0009 0.0009 0.0018 0.002 0.0048 0.0018 0.0005 0.0006 0.0034 0.0013 0.0018
Bud Periphery 0.0014 0 0.0019 0.0002 0.0028 0 0.0013 0.0002 0.0003 0.0001 0.0001 0.0004 0.0012 0.0001 0.0001 0.0024 0.0039 0
Bud Site 0.0088 0.0004 0.0106 0.0008 0.0018 0 0.0017 0.0059 0.0012 0.0004 0.0003 0.0002 0.0086 0.0028 0.0005 0.0028 0.0037 0.0002
Cell Periphery 0.0004 0 0.0004 0.0001 0.0003 0 0.0001 0.0001 0 0 0 0 0.0006 0.0001 0 0.0002 0.0001 0
Cytoplasm 0.7098 0.9598 0.8066 0.8608 0.681 0.9019 0.8457 0.8157 0.7944 0.7701 0.7954 0.8167 0.6841 0.9224 0.9151 0.7138 0.7756 0.9147
Cytoplasmic Foci 0.0352 0.0021 0.0295 0.0045 0.0174 0.0024 0.0122 0.0097 0.0144 0.0085 0.0076 0.0075 0.0318 0.005 0.0103 0.0246 0.0165 0.007
Eisosomes 0.0005 0 0.0003 0 0.0006 0 0 0 0 0 0 0 0.0004 0 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.0104 0.0016 0.0034 0.0043 0.0071 0.003 0.0052 0.0029 0.0023 0.0019 0.0049 0.0029 0.0129 0.0031 0.0024 0.0035 0.0051 0.0032
Endosome 0.0428 0.0006 0.0147 0.0353 0.038 0.0017 0.0065 0.0057 0.0054 0.0042 0.0059 0.0032 0.0686 0.0032 0.0087 0.036 0.0404 0.0036
Golgi 0.009 0.0001 0.009 0.0029 0.0218 0 0.0006 0.0003 0.0009 0.0001 0.0002 0.0001 0.018 0.0004 0.0015 0.0195 0.013 0.0003
Lipid Particles 0.0117 0 0.0076 0.0071 0.0279 0 0.0013 0.0001 0.0006 0 0.0001 0 0.0318 0.0017 0.0006 0.0051 0.0169 0.0001
Mitochondria 0.0047 0.0001 0.0117 0.0026 0.0455 0.0001 0.0008 0.0005 0.0005 0.0003 0.0005 0.0003 0.0118 0.0006 0.0055 0.0768 0.0168 0.0003
None 0.0168 0.0074 0.0241 0.0276 0.0114 0.0056 0.0254 0.0528 0.0172 0.0128 0.006 0.0135 0.0154 0.0109 0.0056 0.004 0.0037 0.0046
Nuclear Periphery 0.0188 0.0019 0.0042 0.005 0.0359 0.0041 0.0077 0.0057 0.0047 0.0049 0.0114 0.0055 0.0162 0.0035 0.0029 0.0031 0.01 0.0044
Nucleolus 0.0005 0 0.0005 0.0004 0.0008 0 0.0003 0.0004 0.0003 0.0003 0.0003 0.0002 0.0031 0.0002 0.0001 0.0002 0.0009 0.0001
Nucleus 0.0293 0.0237 0.0409 0.0358 0.0169 0.0779 0.0726 0.0866 0.1443 0.1766 0.1501 0.1293 0.0314 0.039 0.0411 0.0462 0.0698 0.0477
Peroxisomes 0.016 0.0001 0.0141 0.0006 0.0378 0 0.0014 0.0003 0.0014 0.0002 0.0005 0.0003 0.0207 0.0012 0.0006 0.0176 0.0053 0.0025
Punctate Nuclear 0.0085 0.0012 0.0048 0.0017 0.0051 0.0012 0.009 0.007 0.0062 0.0161 0.0122 0.0122 0.0077 0.003 0.0028 0.0025 0.0065 0.0053
Vacuole 0.0034 0.0003 0.0014 0.0065 0.0037 0.0005 0.0015 0.0025 0.0013 0.001 0.0013 0.001 0.0077 0.0011 0.0009 0.0027 0.0016 0.0007
Vacuole Periphery 0.0015 0 0.0004 0.0019 0.0054 0.0001 0.0003 0.0003 0.0002 0.0002 0.0003 0.0002 0.0032 0.0002 0.0002 0.0018 0.001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.2621 58.349 46.0465 37.7908 30.2242 38.313 57.1237 49.2994 53.4473 41.9175
Translational Efficiency 1.2452 1.0498 0.8973 1.419 1.6051 1.3377 1.1514 1.3467 1.1396 1.0714

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2244 198 2772 101 1331 2041 1200 752 3575 2239 3972 853

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 892.70 1005.25 998.68 1148.96 848.66 937.04 1150.21 1091.29 876.30 943.07 1044.46 1098.12
Standard Deviation 117.72 152.79 148.85 228.25 117.84 131.14 143.34 161.87 119.67 134.60 162.82 172.09
Intensity Change Log 2 — 0.171307 0.161847 0.364081 — 0.142924 0.438639 0.362776 — 0.157544 0.303377 0.363445

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000111 0.004093 0.001042 0.025930 0.000874 0.000342 0.000590 0.018150 0.000395 0.000674 0.000905 0.019071
Bud Neck 0.002456 0.004526 0.006191 0.004389 0.001542 0.005070 0.002466 0.012197 0.002115 0.005022 0.005066 0.011272
Bud Site 0.002538 0.024550 0.016451 0.127451* 0.003021 0.009802 0.004803 0.110450* 0.002718 0.011106 0.012932 0.112463*
Cell Periphery 0.000054 0.000129 0.000105 0.000237 0.000136 0.000072 0.000144 0.000224 0.000084 0.000077 0.000116 0.000225
Cytoplasm 0.724314* 0.618334* 0.594077* 0.133706* 0.690887* 0.714117* 0.426813* 0.200291* 0.711869* 0.705647* 0.543544* 0.192407*
Cytoplasmic Foci 0.040422 0.112297 0.004082 0.117035 0.085020 0.052807 0.006582 0.087001 0.057027 0.058068 0.004837 0.090558
Eisosomes 0.000016 0.000048 0.000012 0.000052 0.000045 0.000022 0.000029 0.000095 0.000027 0.000024 0.000017 0.000090
Endoplasmic Reticulum 0.000484 0.001724 0.001113 0.001385 0.001566 0.000740 0.003206 0.002606 0.000887 0.000827 0.001745 0.002462
Endosome 0.001303 0.008741 0.001055 0.025993 0.004329 0.004842 0.001300 0.066082 0.002429 0.005186 0.001129 0.061336
Golgi 0.000399 0.004906 0.000738 0.005913 0.000643 0.002702 0.000231 0.022394 0.000490 0.002897 0.000584 0.020442
Lipid Particles 0.000486 0.002165 0.000419 0.009262 0.001799 0.001185 0.000962 0.003319 0.000975 0.001272 0.000583 0.004023
Mitochondria 0.000489 0.000334 0.001643 0.000664 0.000657 0.001120 0.000506 0.025374 0.000552 0.001051 0.001300 0.022448
Mitotic Spindle 0.001215 0.013729 0.030864 0.105009* 0.002286 0.009648 0.005990 0.177409* 0.001614 0.010009 0.023349 0.168837*
None 0.005709 0.006133 0.007775 0.001788 0.005519 0.005830 0.008219 0.002100 0.005638 0.005857 0.007909 0.002063
Nuclear Periphery 0.000193 0.000375 0.001581 0.000439 0.000273 0.000390 0.001325 0.001904 0.000223 0.000388 0.001504 0.001731
Nuclear Periphery Foci 0.000624 0.002973 0.000468 0.002678 0.002695 0.001384 0.002500 0.006783 0.001395 0.001525 0.001082 0.006297
Nucleolus 0.000341 0.000305 0.000701 0.000097 0.000340 0.000633 0.000226 0.001549 0.000341 0.000604 0.000557 0.001377
Nucleus 0.185307* 0.103392 0.287957* 0.033670 0.150307* 0.123543 0.456115* 0.034448 0.172277* 0.121761 0.338760* 0.034356
Peroxisomes 0.000444 0.015716 0.000979 0.146579* 0.001072 0.002689 0.001860 0.055127 0.000678 0.003841 0.001245 0.065956
Vacuole 0.032927 0.075264 0.041213 0.228867* 0.046271 0.062281 0.076051 0.155227 0.037895 0.063429 0.051738 0.163946
Vacuole Periphery 0.000170 0.000263 0.001535 0.028856 0.000719 0.000781 0.000081 0.017270 0.000374 0.000736 0.001096 0.018642

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.72 -2.64 -2.23 -1.16 -2.11 0.96 0.27 -3.70 -4.14 -3.93 -1.04 -1.13 -4.57 -4.38 -4.42
Bud Neck -2.59 -9.45 -3.32 -1.18 0.58 -5.92 -7.38 -11.68 -4.59 -7.31 -5.39 -11.42 -11.36 -4.67 -3.85
Bud Site -4.58 -7.24 -3.98 -1.94 -3.27 -5.97 -3.42 -11.13 -9.53 -10.64 -7.71 -7.79 -11.91 -9.72 -10.15
Cell Periphery -4.96 -6.32 -4.07 -1.19 -2.51 7.62 -2.15 -2.03 -7.34 -0.51 1.33 -5.61 -7.07 -7.47 -4.21
Cytoplasm 6.63 35.93 23.91 12.46 12.53 -0.62 40.10 37.63 39.43 5.49 3.90 49.53 48.36 41.70 18.18
Cytoplasmic Foci -5.55 21.58 -0.38 3.89 -3.96 8.15 24.51 9.59 3.09 -11.29 -0.22 32.00 4.44 4.23 -12.26
Eisosomes -4.68 -3.14 -7.82 -1.32 -7.35 7.28 1.54 -3.50 -6.80 -4.34 0.64 0.90 -6.96 -7.10 -7.24
Endoplasmic Reticulum -4.81 -16.01 -5.02 -0.10 -0.89 3.21 -8.40 -1.29 -5.54 7.63 0.02 -13.19 -5.26 -5.58 2.32
Endosome -2.28 1.67 -3.45 -0.43 -3.66 -0.57 9.04 -5.87 -5.62 -7.23 -4.41 7.08 -7.04 -5.65 -7.75
Golgi -1.37 0.23 -2.00 0.93 -1.97 -2.63 2.83 -3.32 -1.76 -3.71 -3.17 1.06 -3.53 -1.56 -3.68
Lipid Particles -4.09 -5.15 -2.92 -2.17 -2.82 2.31 2.44 -6.10 -7.45 -8.88 -1.32 0.80 -8.44 -6.82 -8.72
Mitochondria 1.66 -4.94 -0.28 -1.03 2.81 -1.25 -0.13 -2.71 -2.52 -2.72 -2.23 -3.91 -2.78 -2.52 -2.41
Mitotic Spindle -2.15 -12.29 -4.35 -3.67 -2.90 -3.71 -2.94 -11.96 -11.01 -11.19 -5.04 -12.44 -12.87 -11.63 -9.65
None 0.33 -5.03 4.98 2.01 9.88 0.10 -1.35 1.27 1.38 4.33 0.27 -4.33 2.67 1.74 7.68
Nuclear Periphery -4.93 -14.37 -7.46 -5.47 6.18 -2.52 -29.83 -10.07 -8.82 1.23 -3.67 -19.39 -11.02 -9.30 2.58
Nuclear Periphery Foci -5.84 0.30 -2.83 -0.84 -2.84 3.26 -0.64 -1.52 -3.04 -1.20 -0.81 0.70 -3.82 -3.23 -3.96
Nucleolus 1.00 -1.64 0.13 -0.83 1.70 -2.19 0.07 -3.75 -1.12 -3.41 -2.05 -1.60 -3.63 -1.07 -1.85
Nucleus 6.68 -29.54 8.13 2.41 20.33 4.10 -39.42 5.79 3.09 39.87 11.29 -45.67 11.24 3.75 42.63
Peroxisomes -3.40 -0.49 -2.90 -1.57 -2.89 -2.78 0.53 -6.52 -5.89 -6.61 -5.18 0.12 -7.26 -6.04 -7.26
Vacuole -6.47 -15.83 -12.04 -9.17 -10.44 -7.31 -15.55 -25.36 -22.63 -15.55 -12.27 -21.50 -28.88 -24.45 -22.38
Vacuole Periphery -1.35 -1.79 -1.00 -0.98 -0.89 -0.01 2.14 -3.10 -3.15 -3.88 -1.31 -0.57 -3.49 -3.15 -3.32
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein involved in membrane-trafficking events at plasma membrane; interacts with actin regulators Sla1p and Las17p, ubiquitin, Arf3p to couple actin dynamics to membrane trafficking processes; similar structure to GGA family of proteins with N-terminal VHS domain and GAT domain; binds Las17p, which is homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly, actin polymerization; may mediate disassembly of Pan1 complex from endocytic coat
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Lsb5

Lsb5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lsb5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available