Standard name
Human Ortholog
Description Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.96 0.93 0.94 – 0.77 0.81 0.8 0.81 0.75 0.99 0.98 0.98 0.98 0.95 0.95 0.95 0.93 0.92 0.93 0.86 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.05 0 0.07 0.06 0.07 0 0 0 0.06 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0.05 – 0.17 0.1 0.08 0.06 0.07 0 0 0 0.05 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 3 4 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 2 0 7 0 0 0 – 0 1 3 0 2 3 0 2 0 0 0 1 1 3 0 11 14
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 0 2 3 2 – 7 13 10 8 16 0 3 3 0 3 1 0 0 0 0 0 0
Cytoplasm 480 295 251 262 307 274 – 339 256 279 242 246 387 392 501 64 86 56 472 271 241 202 304 308
Endoplasmic Reticulum 0 2 0 4 2 3 – 21 13 23 17 22 3 3 2 4 6 2 0 0 0 0 3 3
Endosome 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 2 3 6 3 2 0 0 0 0 0 0 0 0 0 1 0 0
Mitochondria 0 1 0 1 0 1 – 8 10 7 14 19 0 1 0 2 2 0 0 0 0 0 1 0
Nucleus 19 2 0 0 0 1 – 7 5 5 5 7 1 0 3 3 0 1 11 8 3 4 9 7
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2 0 2 – 0 2 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Vac/Vac Membrane 1 1 3 9 23 16 – 75 33 28 17 24 2 6 8 3 6 2 6 6 5 5 15 9
Unique Cell Count 489 299 253 274 329 293 441 317 347 300 329 391 402 509 65 91 59 497 292 262 218 353 353
Labelled Cell Count 505 302 261 280 335 299 462 340 365 310 340 396 406 519 76 103 63 497 292 262 218 353 353


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.8 20.5 14.8 20.5 25.2 24.9 – 27.6 28.6 29.9 28.9 29.9 23.6 27.4 30.7 24.8 27.3 28.6 19.3 22.3 25.4
Std Deviation (1e-4) 3.9 5.0 4.3 6.1 6.2 6.9 – 9.2 10.2 9.8 10.7 17.6 5.6 8.3 9.4 14.3 12.2 16.5 4.7 8.1 8.4
Intensity Change (Log2) – – – 0.47 0.77 0.76 – 0.9 0.95 1.02 0.97 1.02 0.67 0.89 1.06 0.75 0.89 0.95 0.39 0.59 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 3.3 0 0 3.6 0 0 0 0 0 0
Cytoplasm -2.6 -3.5 -3.5 – -7.9 -7.0 -7.1 -7.0 -8.3 -0.3 -1.6 -0.9 -0.6 -2.7 -2.4
Endoplasmic Reticulum 0 0 0 – 3.5 3.3 4.2 3.8 4.2 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 3.5 3.9 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.6 3.4 2.7 – 6.4 4.5 3.8 2.8 3.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.8185 16.9052 15.8306 14.9846 12.1456 15.868 12.6965 17.0334 14.1907 12.3926 12.9742 14.6351 13.6895 17.3835 15.0966 16.1348 14.8081 16.5723
Actin 0.0059 0.0059 0.0207 0.0285 0.0533 0.0019 0.0109 0.0011 0.0041 0.0062 0.0016 0.0007 0.0141 0.0002 0.0049 0.0002 0.0001 0.0006
Bud 0.0003 0.0002 0.0004 0.0001 0.0004 0.0003 0.0011 0.0032 0.0002 0.0008 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0 0.0001
Bud Neck 0.0005 0.0002 0.001 0.0005 0.0015 0.0011 0.0011 0.0003 0.0005 0.002 0.0008 0.0009 0.0012 0.0002 0.0007 0.0005 0.0006 0.0016
Bud Periphery 0.0002 0.0001 0.0007 0.0001 0.0005 0.0002 0.0015 0.0004 0.0001 0.0005 0.0001 0 0.0002 0 0.0001 0 0 0
Bud Site 0.0003 0.0006 0.0005 0.0003 0.0012 0.0004 0.0037 0.0016 0.0009 0.0004 0.0002 0.0001 0.0003 0.0001 0.0008 0 0 0
Cell Periphery 0.0001 0 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0001 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8893 0.9519 0.9237 0.9089 0.7955 0.8826 0.7946 0.9624 0.892 0.8007 0.8461 0.8979 0.8468 0.9866 0.9031 0.9167 0.8958 0.9223
Cytoplasmic Foci 0.0028 0.0019 0.0019 0.0042 0.0103 0.0022 0.0188 0.0031 0.0119 0.0378 0.0088 0.0051 0.0181 0.0006 0.0085 0.001 0.0005 0.0017
Eisosomes 0.0001 0.0002 0.0003 0.0001 0.0004 0.0016 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0039 0.0005 0.0008 0.0005 0.0067 0.0032 0.0067 0.0016 0.0019 0.0024 0.0025 0.0017 0.0037 0.0006 0.0025 0.0016 0.0019 0.0023
Endosome 0.0103 0.0004 0.0032 0.0017 0.0147 0.007 0.0239 0.0012 0.0156 0.0313 0.0351 0.0098 0.0141 0.0001 0.0044 0.0004 0.0004 0.001
Golgi 0.0013 0.0004 0.0012 0.0024 0.0127 0.0006 0.0074 0.0004 0.0088 0.0271 0.0206 0.0043 0.005 0 0.0017 0 0 0
Lipid Particles 0.0009 0.0025 0.002 0.0038 0.0239 0.0004 0.0055 0.0008 0.0067 0.0045 0.0143 0.001 0.0031 0 0.0045 0 0 0
Mitochondria 0.0012 0.0021 0.0022 0.0005 0.0035 0.003 0.0165 0.0002 0.0023 0.008 0.0039 0.0008 0.0015 0 0.0009 0.0001 0.0001 0.0002
None 0.0063 0.0012 0.0042 0.0008 0.0028 0.0138 0.002 0.0012 0.0021 0.0018 0.0007 0.0005 0.0037 0.0005 0.0024 0.0005 0.0004 0.0007
Nuclear Periphery 0.0121 0.0018 0.0031 0.003 0.0128 0.0078 0.0152 0.002 0.0038 0.0045 0.0075 0.0079 0.009 0.0012 0.0061 0.0059 0.0086 0.0069
Nucleolus 0.0007 0.0001 0.0006 0 0.0003 0.0006 0.0006 0 0.0001 0.0001 0.0001 0 0.0005 0 0.0002 0 0 0
Nucleus 0.0546 0.0265 0.0263 0.0374 0.0444 0.067 0.063 0.019 0.0432 0.0588 0.0528 0.0631 0.0501 0.0095 0.0496 0.0719 0.0899 0.0608
Peroxisomes 0.0002 0.003 0.0008 0.0054 0.0082 0.0002 0.0083 0.0003 0.0033 0.0044 0.001 0.0026 0.0112 0 0.0049 0 0 0
Punctate Nuclear 0.0071 0.0005 0.001 0.0014 0.0051 0.0015 0.0046 0.0005 0.0011 0.007 0.0012 0.0015 0.0156 0.0002 0.0039 0.0006 0.0008 0.001
Vacuole 0.0014 0.0002 0.0042 0.0002 0.0008 0.0033 0.0109 0.0005 0.001 0.0008 0.0017 0.0016 0.0008 0.0001 0.0004 0.0003 0.0006 0.0005
Vacuole Periphery 0.0006 0.0001 0.0011 0.0001 0.001 0.0013 0.0032 0.0001 0.0003 0.0005 0.0009 0.0004 0.0004 0 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 140.8249 74.0523 72.534 125.9234 79.4784 139.0911 119.7987 135.4338 145.6113 147.2728
Translational Efficiency 2.8776 3.9848 3.6285 2.369 4.5895 3.8385 3.1216 2.7013 3.0629 3.0992

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
146 1694 1914 571 1657 1954 247 1732 1803 3648 2161 2303

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1325.86 1605.55 1930.21 3391.60 1286.34 1495.30 2287.99 3068.50 1289.54 1546.50 1971.10 3148.61
Standard Deviation 288.64 325.69 442.08 1007.73 232.06 256.66 448.41 877.56 237.39 295.92 457.20 922.18
Intensity Change Log 2 — 0.276139 0.541829 1.355038 — 0.217163 0.830809 1.254262 — 0.247398 0.691362 1.306291

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000423 0.000166 0.000145 0.001479 0.000151 0.000132 0.000590 0.000334 0.000173 0.000148 0.000196 0.000617
Bud Neck 0.001498 0.000816 0.000825 0.003280 0.000468 0.001285 0.003667 0.002226 0.000551 0.001067 0.001150 0.002487
Bud Site 0.009239 0.003931 0.000991 0.003771 0.002369 0.003321 0.004572 0.010365 0.002926 0.003604 0.001400 0.008730
Cell Periphery 0.000343 0.000079 0.000100 0.000612 0.000058 0.000041 0.000580 0.000233 0.000081 0.000058 0.000155 0.000327
Cytoplasm 0.819229* 0.789775* 0.535624* 0.498304* 0.833556* 0.821992* 0.382715* 0.647269* 0.832396* 0.807032* 0.518147* 0.610335*
Cytoplasmic Foci 0.039595 0.009442 0.002412 0.010262 0.014933 0.011834 0.019670 0.012661 0.016930 0.010723 0.004384 0.012066
Eisosomes 0.000008 0.000002 0.000001 0.000010 0.000003 0.000003 0.000009 0.000002 0.000003 0.000003 0.000002 0.000004
Endoplasmic Reticulum 0.001004 0.000218 0.000122 0.000273 0.000467 0.000325 0.000608 0.000138 0.000510 0.000275 0.000177 0.000171
Endosome 0.001705 0.000806 0.000084 0.005296 0.000339 0.001445 0.005789 0.002410 0.000450 0.001148 0.000736 0.003126
Golgi 0.000985 0.000542 0.000109 0.024795 0.000359 0.002332 0.001834 0.008186 0.000409 0.001501 0.000307 0.012304
Lipid Particles 0.000519 0.000198 0.000207 0.001308 0.000224 0.000202 0.001755 0.000382 0.000248 0.000200 0.000384 0.000611
Mitochondria 0.004018 0.000175 0.000271 0.011290 0.000448 0.000564 0.001127 0.000453 0.000737 0.000383 0.000369 0.003140
Mitotic Spindle 0.013578 0.007581 0.003701 0.046303 0.002323 0.008295 0.002962 0.019976 0.003234 0.007963 0.003617 0.026503
None 0.002841 0.015127 0.012665 0.009365 0.006347 0.010876 0.007655 0.014648 0.006063 0.012850 0.012092 0.013338
Nuclear Periphery 0.000133 0.000101 0.000264 0.000578 0.000120 0.000095 0.000821 0.000246 0.000121 0.000098 0.000328 0.000328
Nuclear Periphery Foci 0.005094 0.002285 0.000519 0.001597 0.002549 0.001673 0.003399 0.001298 0.002755 0.001957 0.000848 0.001372
Nucleolus 0.000095 0.000187 0.000089 0.000154 0.000234 0.000303 0.000165 0.000115 0.000223 0.000249 0.000098 0.000125
Nucleus 0.074082 0.159590* 0.435682* 0.345846* 0.120402 0.122945 0.466584* 0.266724* 0.116651 0.139961* 0.439214* 0.286342*
Peroxisomes 0.000240 0.000110 0.000012 0.000187 0.000047 0.000262 0.000096 0.000181 0.000062 0.000192 0.000021 0.000183
Vacuole 0.024161 0.008661 0.006140 0.031234 0.012929 0.010826 0.074931 0.010444 0.013838 0.009821 0.014003 0.015599
Vacuole Periphery 0.001209 0.000208 0.000035 0.004056 0.001675 0.001250 0.020471 0.001708 0.001637 0.000766 0.002371 0.002290

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.94 1.99 0.32 -2.11 -1.31 -0.39 -9.14 -1.78 -1.39 7.04 -0.15 -5.67 -2.30 -2.18 1.23
Bud Neck 1.05 -0.35 -5.45 -9.76 -8.16 -1.79 -9.68 -6.93 -1.80 7.52 -2.19 -12.14 -10.88 -5.78 -1.50
Bud Site 2.68 3.86 1.81 -2.48 -9.22 -1.31 -6.08 -3.58 -2.34 3.41 -0.98 0.49 -3.58 -2.79 -5.00
Cell Periphery 1.77 1.16 -3.60 -11.49 -10.31 2.12 -10.35 -13.47 -14.12 6.85 1.25 -9.74 -15.02 -17.11 -8.05
Cytoplasm 1.24 17.82 17.33 30.31 2.06 2.78 36.01 30.54 27.95 -20.96 6.23 59.11 38.72 36.94 -17.80
Cytoplasmic Foci 6.35 7.94 3.93 -5.81 -10.63 2.56 -2.89 1.43 -1.18 3.55 5.11 10.11 1.58 -3.98 -10.00
Eisosomes 3.05 2.96 0.08 -8.39 -8.23 -0.01 -11.24 -2.07 -1.63 10.83 1.72 -3.61 -4.07 -6.59 -0.38
Endoplasmic Reticulum 3.07 3.27 3.71 4.39 5.48 1.93 -2.69 7.21 4.24 9.35 3.49 4.29 7.92 5.97 11.37
Endosome 1.81 3.11 1.83 0.24 -1.62 -3.54 -2.32 -0.53 2.74 2.09 -3.20 1.50 -0.20 2.43 -1.00
Golgi 1.59 2.95 -1.07 -1.39 -1.61 -2.71 -2.37 -1.66 0.59 -0.97 -2.64 1.46 -2.13 -0.63 -2.39
Lipid Particles 2.84 -0.17 -8.92 -10.97 -9.49 0.14 -10.40 -8.90 -8.90 7.97 0.44 -10.72 -13.36 -13.72 -4.80
Mitochondria 1.00 0.94 0.63 -1.21 -1.00 -0.21 -0.66 1.01 1.26 3.90 0.94 0.85 0.48 -0.53 -0.38
Mitotic Spindle 0.77 1.13 0.27 -1.12 -2.08 -3.41 -2.88 -3.95 -0.28 -1.74 -3.35 -1.64 -3.91 -0.80 -2.53
None -13.54 -16.71 -8.82 6.77 5.99 -5.50 -0.01 -8.19 -1.93 -7.36 -9.26 -8.78 -8.30 2.34 0.35
Nuclear Periphery 0.91 -10.02 -13.32 -15.93 -7.97 0.34 -16.27 -7.08 -12.53 14.14 0.30 -15.79 -11.94 -19.38 4.96
Nuclear Periphery Foci 2.63 4.44 3.36 1.94 -5.48 2.19 -3.49 6.44 3.70 7.41 2.02 6.32 6.62 4.94 0.60
Nucleolus -2.83 -1.99 -4.96 0.30 -5.22 -0.35 0.17 1.62 1.89 9.95 -0.12 1.22 1.37 2.21 1.77
Nucleus -10.02 -38.56 -18.85 -14.84 15.55 -1.82 -18.53 -25.12 -24.48 8.85 -7.99 -61.38 -30.65 -26.86 29.38
Peroxisomes 1.37 2.64 2.31 2.12 -1.56 -2.05 -4.39 -0.02 2.02 3.02 -2.26 3.58 0.93 2.47 -0.96
Vacuole 3.28 2.25 -9.08 -17.88 -16.53 1.02 -12.41 -14.55 -15.51 9.17 3.06 -8.44 -19.90 -22.85 -11.04
Vacuole Periphery 1.49 1.71 0.96 -1.31 -1.93 0.73 -0.36 2.02 1.88 0.94 1.51 2.01 2.07 1.24 -0.15
Standard name
Human Ortholog
Description Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Grx1

Grx1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Grx1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available