Standard name
Human Ortholog
Description Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.06 0.05 0.07 0.05 0.07 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Cytoplasm 0.99 0.94 0.97 0.97 0.94 0.92 0.79 0.71 0.69 0.61 0.57 0.55 0.99 1.0 0.57 0.91 0.82 0.91 0.96 0.94 0.89 0.92 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.26 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0.06 0.18 0.21 0.26 0.36 0.41 0.44 0.47 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.09 0 0.09 0.06 0.08 0 0 0 0 0.1 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 3 4 4 4 3 10 0 0 11 0 2 0 0 1 2 0 3 4
Bud 0 2 9 0 2 1 6 10 2 9 8 1 6 3 35 0 1 0 0 0 0 1 7 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 2
Bud Site 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 5 3 5 13 15 28 42 23 40 20 34 11 31 12 13 10 9 0 0 0 1 0 0
Cytoplasm 103 258 262 276 447 480 515 494 315 366 219 263 499 758 450 302 170 132 99 273 244 186 424 453
Endoplasmic Reticulum 0 1 1 2 1 9 24 21 23 21 23 19 1 1 3 9 6 5 0 1 6 4 9 6
Endosome 0 0 0 0 2 0 4 3 0 1 0 2 0 1 210 11 9 0 0 1 0 0 3 2
Golgi 0 0 0 0 2 0 0 0 0 0 0 1 0 0 1 1 4 0 0 1 0 0 3 1
Mitochondria 5 11 9 12 30 93 139 177 163 248 169 224 1 0 0 19 8 6 0 3 4 0 6 3
Nucleus 0 0 0 1 0 2 1 1 3 1 3 1 0 1 0 0 1 3 0 0 0 0 0 1
Nuclear Periphery 0 6 0 0 0 1 0 2 4 8 4 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 2 0 0 0 1 3 0 1 0 1 0 0 23 6 3 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 1 0 0 0 0 0 0 0 105 1 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 1 0 1 0 0 119 9 6 2 0 1 1 0 1 2
Vac/Vac Membrane 0 2 0 0 7 2 26 61 20 53 24 39 1 1 15 13 20 8 0 4 6 2 3 7
Unique Cell Count 104 275 270 284 474 524 652 692 459 600 381 481 502 761 793 333 207 145 104 291 275 202 471 497
Labelled Cell Count 109 286 286 296 506 603 748 818 558 754 473 596 519 796 984 384 241 168 104 291 275 202 471 497


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.8 6.6 6.1 6.9 7.3 7.4 7.9 8.0 8.1 8.2 8.6 7.9 9.4 10.9 9.7 11.5 12.0 6.3 6.3 7.0
Std Deviation (1e-4) 1.0 1.1 1.9 1.4 1.7 1.6 1.7 2.1 2.5 2.5 2.8 3.2 1.6 2.2 2.4 2.4 3.3 3.0 1.8 1.5 1.3
Intensity Change (Log2) – – – -0.1 0.06 0.14 0.17 0.26 0.27 0.29 0.31 0.38 0.27 0.51 0.72 0.56 0.8 0.86 -0.08 -0.07 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 -1.9 0 -1.8 -1.0 0 -2.1 0 0.8 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.5 1.6 2.4 3.3 2.7 3.5 2.8 3.6 0 2.3 0 2.1 2.5 0
Cytoplasm 0.1 -1.7 -2.9 -6.9 -8.7 -9.1 -11.0 -11.3 -12.1 2.6 3.5 -12.2 -3.2 -5.5 -2.7
Endoplasmic Reticulum 0 0 0 2.8 2.5 3.4 2.7 3.8 2.9 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 9.4 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 1.8 5.8 6.8 7.8 9.9 11.4 11.6 12.3 0 0 0 1.4 0.3 0.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 2.0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 6.3 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 6.8 0 0 0
Vacuole 0 0 0 3.3 5.0 3.5 5.0 4.2 4.8 0 0 0 3.3 5.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7836 4.5296 4.3662 3.3387 3.7227 3.9183 3.6115 5.2413 4.8328 3.9303 4.3774 4.729 6.5935 7.8837 7.9661 8.2862 5.672 8.2679
Actin 0.0072 0.0008 0.0125 0.0037 0.0003 0.0031 0.0463 0.014 0.0108 0.0142 0.0199 0.002 0.1062 0.0019 0.0364 0.0053 0.0382 0.0005
Bud 0.0003 0.0007 0.0017 0.0021 0.0002 0.0003 0.0003 0.0002 0.0007 0.0005 0.0007 0.0002 0.0003 0.0002 0.0008 0.0013 0.0007 0.0001
Bud Neck 0.0019 0.0004 0.0038 0.0011 0.0003 0.0026 0.0009 0.0004 0.0008 0.0245 0.0007 0.0006 0.0019 0.0002 0.0004 0.0005 0.0008 0.0005
Bud Periphery 0.0003 0.0003 0.0008 0.0011 0.0001 0.0002 0.0004 0.0002 0.0004 0.0007 0.0006 0.0003 0.0004 0.0001 0.0006 0.0003 0.0002 0
Bud Site 0.0012 0.0038 0.0047 0.0008 0.0001 0.0004 0.0014 0.0037 0.009 0.0047 0.0027 0.0001 0.0018 0.0005 0.0045 0.0003 0.0007 0.0001
Cell Periphery 0.0005 0.0004 0.0003 0.0003 0.0002 0.0001 0.0003 0.0004 0.0003 0.0005 0.0001 0.0001 0.0006 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.8013 0.938 0.8745 0.8854 0.9583 0.8962 0.7054 0.9339 0.8197 0.6455 0.8449 0.9394 0.8437 0.951 0.9143 0.9118 0.7881 0.9572
Cytoplasmic Foci 0.0306 0.0079 0.0237 0.0184 0.0073 0.0316 0.0593 0.0036 0.0329 0.0589 0.0192 0.0262 0.0031 0.0025 0.0103 0.0216 0.0595 0.0278
Eisosomes 0.0003 0 0.0001 0.0005 0 0.0001 0.0013 0.0004 0.0001 0.0002 0.0001 0 0.002 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0159 0.0035 0.0108 0.0044 0.0034 0.0042 0.0148 0.0043 0.0068 0.0035 0.0068 0.0041 0.0048 0.0336 0.004 0.0032 0.0055 0.0053
Endosome 0.033 0.0046 0.0228 0.0271 0.0084 0.0141 0.0373 0.0034 0.0166 0.0708 0.0204 0.0093 0.0029 0.0024 0.0094 0.0176 0.0445 0.0029
Golgi 0.0047 0.0015 0.0037 0.0045 0.0003 0.0047 0.0086 0.0005 0.0127 0.059 0.0101 0.0071 0.0042 0.0005 0.0049 0.0022 0.0112 0.0003
Lipid Particles 0.0028 0.0003 0.0033 0.0029 0 0.0027 0.0216 0.0003 0.0058 0.0181 0.0025 0.0001 0.0031 0.0001 0.001 0.001 0.0238 0.0001
Mitochondria 0.0165 0.0002 0.0105 0.0216 0.0001 0.001 0.0137 0.0022 0.002 0.0457 0.0354 0.0011 0.001 0.0002 0.0046 0.0009 0.0007 0.0001
None 0.0653 0.0318 0.0142 0.0093 0.0048 0.0278 0.0685 0.0295 0.0538 0.0031 0.0234 0.0058 0.0203 0.0027 0.003 0.022 0.0052 0.0009
Nuclear Periphery 0.004 0.0006 0.0013 0.0021 0.0012 0.0012 0.0033 0.0003 0.0009 0.0013 0.0021 0.0005 0.0007 0.0016 0.0006 0.0007 0.0019 0.0004
Nucleolus 0.0004 0.0001 0.0001 0.0002 0 0.0005 0.0004 0.0001 0.0001 0.0008 0.0001 0 0 0 0.0001 0 0.0001 0
Nucleus 0.0016 0.0015 0.0016 0.0025 0.0015 0.0021 0.0011 0.0006 0.0009 0.0015 0.001 0.0007 0.0006 0.0009 0.0007 0.0011 0.0011 0.0007
Peroxisomes 0.0035 0.0002 0.0034 0.0032 0 0.0013 0.0079 0.0004 0.0121 0.0355 0.0043 0.0011 0.0014 0 0.0026 0.0006 0.0078 0.0022
Punctate Nuclear 0.0014 0.0002 0.0005 0.0005 0.0001 0.0013 0.0049 0.0002 0.0111 0.0046 0.0019 0.0003 0.0002 0.0001 0.0004 0.0004 0.006 0.0002
Vacuole 0.0054 0.0029 0.0047 0.0065 0.0118 0.0038 0.0019 0.0011 0.0023 0.0048 0.0024 0.0005 0.0006 0.0012 0.001 0.0062 0.0029 0.0006
Vacuole Periphery 0.002 0.0003 0.0008 0.0017 0.0018 0.0006 0.0006 0.0001 0.0003 0.0015 0.0008 0.0001 0.0001 0.0001 0.0002 0.0027 0.0009 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 143.8904 74.1587 130.5111 255.3138 144.2034 289.8227 177.8329 256.6211 398.5894 197.337
Translational Efficiency 1.1852 1.0354 1.2719 1.8051 1.3805 1.2924 1.3356 1.7289 1.4194 1.3632

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Glk1

Glk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Glk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available