Standard name
Human Ortholog
Description Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0.06 0.05 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.05 0 0 0 0 0 0
Cytoplasm 0 0.1 0.07 0.06 0.05 0.05 0 0 0 0 0 0 0.4 0.51 0.54 0.16 0.13 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0.16 0 0.11 0 0 0 0 0 0 0 0.15 0.15 0.18 0.17 0.15 0.18 0.09 0.06 0.1 0.06 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.13 0.1 0 0 0 0 0 0
Mitochondria 0.93 0.95 0.74 0.91 0.83 0.87 0.92 0.91 0.95 0.96 0.96 0.95 0.25 0.1 0.09 0.13 0.12 0.14 0.75 0.7 0.73 0.75 0.68 0.66
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.16 0.08 0.09 0 0 0 0 0 0 0 0 0.05 0.12 0 0 0.06 0 0 0.05 0 0 0
SpindlePole 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.06 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.08 0.12 0.06 0.11 0.08 0.05 0.07 0.07 0.06 0.07 0.05 0.14 0.2 0.16 0.31 0.31 0.31 0 0.1 0 0.06 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 14 4 8 3 10 6 4 1 3 0 11 11
Bud 4 0 5 6 10 6 21 26 22 28 16 22 6 4 4 5 12 13 1 0 1 0 8 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 0 0 0 3 0 2 1 8 10
Bud Site 0 0 1 2 1 2 11 10 9 8 7 6 0 0 0 0 1 4 – – – – – –
Cell Periphery 0 0 7 9 4 1 2 2 5 1 2 5 9 8 11 14 17 12 0 0 0 0 1 1
Cytoplasm 6 4 19 14 16 17 17 20 10 20 5 15 131 183 284 30 29 32 1 3 0 4 23 14
Endoplasmic Reticulum 6 2 1 0 2 2 1 2 2 0 0 1 9 15 8 13 11 17 2 0 1 0 3 3
Endosome 7 0 43 8 37 9 11 11 0 1 2 1 48 53 93 32 32 42 22 5 27 7 27 23
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 2 3 4 10 29 24 3 0 8 0 6 2
Mitochondria 200 38 192 201 268 306 453 566 418 618 258 405 83 37 47 25 26 32 183 57 206 105 362 306
Nucleus 6 0 1 1 2 11 16 20 9 9 4 4 2 3 2 10 9 9 0 1 0 0 7 9
Nuclear Periphery 5 0 0 3 1 5 5 10 4 8 5 13 1 0 0 3 0 2 0 0 2 1 1 1
Nucleolus 3 0 2 2 2 5 9 11 2 9 4 1 0 0 0 2 2 6 1 1 1 1 3 4
Peroxisomes 2 0 43 17 28 9 10 12 5 4 2 4 8 17 64 7 8 14 6 0 14 1 5 6
SpindlePole 3 0 22 7 8 10 15 20 16 22 2 10 10 18 18 9 13 11 2 0 3 3 11 9
Vac/Vac Membrane 21 3 32 13 36 27 26 41 29 40 20 20 47 73 83 60 68 72 9 8 6 8 42 43
Unique Cell Count 215 40 261 221 322 351 494 620 438 642 268 427 328 361 523 191 217 233 246 83 283 140 533 462
Labelled Cell Count 263 47 368 283 415 410 598 751 531 768 327 507 371 420 628 223 267 296 246 83 283 140 533 462


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.5 4.8 4.6 4.6 4.5 4.2 4.2 4.1 4.0 3.8 4.0 6.0 5.9 5.6 6.6 7.2 7.0 5.4 5.9 6.0
Std Deviation (1e-4) 0.8 0.6 1.3 1.6 1.0 1.9 1.2 1.6 1.1 1.3 1.6 1.5 1.0 0.8 0.8 1.3 1.5 1.6 1.1 1.0 1.2
Intensity Change (Log2) – – – -0.06 -0.05 -0.11 -0.21 -0.19 -0.25 -0.28 -0.34 -0.25 0.31 0.28 0.22 0.45 0.58 0.55 0.18 0.29 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.0 5.1 5.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3461 2.5773 2.5166 1.8684 1.6902 2.5033 0.4224 0.8445 0.5853 0.3343 0.0966 0.4294 -0.0852 0.3816 0.1592 0.3483 -0.0705 0.2008
Actin 0.0064 0.0867 0.0152 0.0015 0.0005 0.0109 0.0522 0.0207 0.013 0.0012 0.0274 0.0097 0.0157 0.0168 0.0246 0.0196 0.0043 0.0044
Bud 0.0007 0.0012 0.0044 0.0011 0.0023 0.0007 0.0023 0.0044 0.0071 0.0005 0.0007 0.0021 0.0017 0.0042 0.005 0.0058 0.0033 0.0024
Bud Neck 0.0003 0.0016 0.002 0.0056 0.0002 0.0145 0.0032 0.0011 0.0025 0.0003 0.0025 0.0198 0.0031 0.0027 0.002 0.0234 0.0018 0.0022
Bud Periphery 0.0017 0.0023 0.0113 0.0039 0.0056 0.0015 0.0041 0.0056 0.0058 0.001 0.0011 0.0035 0.0032 0.0047 0.0078 0.0113 0.0075 0.0032
Bud Site 0.0018 0.0358 0.019 0.0031 0.0015 0.0019 0.0125 0.0385 0.0166 0.0005 0.0038 0.0024 0.0054 0.0376 0.0102 0.0287 0.0061 0.0011
Cell Periphery 0.0008 0.0007 0.0014 0.0041 0.0009 0.0007 0.0027 0.001 0.0012 0.0002 0.0005 0.0007 0.0011 0.0023 0.0014 0.0042 0.0012 0.0004
Cytoplasm 0.0137 0.013 0.0344 0.0052 0.003 0.0456 0.0132 0.0289 0.0229 0.0095 0.0076 0.0168 0.0109 0.0338 0.041 0.0242 0.0082 0.044
Cytoplasmic Foci 0.0083 0.1016 0.0727 0.0186 0.0047 0.0467 0.0687 0.0563 0.0491 0.021 0.1086 0.0478 0.0678 0.0551 0.0584 0.023 0.0396 0.0473
Eisosomes 0.0004 0.0007 0.0005 0.0011 0.0001 0.0003 0.0008 0.0006 0.0003 0.0001 0.0002 0.0002 0.0005 0.0007 0.0004 0.0003 0.0002 0.0002
Endoplasmic Reticulum 0.0265 0.0044 0.0042 0.0008 0.0013 0.0018 0.0063 0.0044 0.0043 0.0014 0.003 0.004 0.0104 0.0041 0.0119 0.0029 0.0051 0.0053
Endosome 0.0828 0.1937 0.1258 0.0868 0.0912 0.0969 0.1866 0.1572 0.1461 0.1036 0.2129 0.1219 0.1845 0.1992 0.1807 0.1176 0.1177 0.156
Golgi 0.0157 0.0629 0.0676 0.051 0.0302 0.0587 0.1004 0.0675 0.0854 0.0369 0.1478 0.0814 0.0922 0.1047 0.075 0.0526 0.0493 0.0868
Lipid Particles 0.2099 0.0072 0.0158 0.0135 0.0009 0.0139 0.0568 0.0095 0.0189 0.0036 0.0555 0.0065 0.0678 0.0145 0.0251 0.0164 0.0085 0.0158
Mitochondria 0.1235 0.3737 0.4623 0.6974 0.8064 0.618 0.372 0.5344 0.537 0.6973 0.3509 0.6267 0.4192 0.3918 0.4595 0.5652 0.7074 0.5753
None 0.3284 0.089 0.049 0.0749 0.0004 0.0402 0.0101 0.0026 0.0127 0.0461 0.0011 0.0014 0.0062 0.0161 0.0082 0.0007 0.0011 0.0053
Nuclear Periphery 0.0929 0.0015 0.0018 0.0005 0.0005 0.0006 0.0187 0.0022 0.0056 0.0015 0.001 0.003 0.0176 0.0045 0.0163 0.0014 0.0032 0.0014
Nucleolus 0.002 0.0003 0.0011 0.0011 0.0002 0.0015 0.0017 0.0013 0.0023 0.0002 0.0138 0.0008 0.0035 0.0046 0.0012 0.0005 0.0004 0.0004
Nucleus 0.0089 0.0005 0.0014 0.0008 0.0004 0.0019 0.0056 0.0009 0.0017 0.0013 0.0027 0.0011 0.0104 0.0019 0.0081 0.0022 0.0016 0.0011
Peroxisomes 0.0194 0.0094 0.0704 0.0094 0.0017 0.024 0.0341 0.0303 0.0328 0.0204 0.0257 0.0145 0.0376 0.0186 0.0203 0.0527 0.007 0.0162
Punctate Nuclear 0.0014 0.0011 0.0016 0.0002 0 0.0007 0.0088 0.0003 0.0005 0.0001 0.0016 0.0002 0.0107 0.0003 0.0066 0.0002 0.0058 0.0006
Vacuole 0.024 0.0079 0.0284 0.0089 0.0292 0.0139 0.0244 0.0228 0.0243 0.0408 0.0194 0.0169 0.0188 0.0592 0.0232 0.0292 0.0111 0.0172
Vacuole Periphery 0.0303 0.0048 0.0097 0.0102 0.0188 0.005 0.0148 0.0094 0.0097 0.0125 0.0122 0.0185 0.0117 0.0225 0.0129 0.0179 0.0094 0.0133

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9584 13.3719 18.6777 21.1859 13.6901 18.4678 16.7016 15.0299 19.0534 18.3379
Translational Efficiency 1.2947 1.4683 1.1691 1.0062 1.3886 1.19 1.2424 1.4811 1.1363 1.0867

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
922 292 1678 1196 1140 1353 386 1376 2062 1645 2064 2572

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 644.26 829.40 876.23 844.20 726.08 781.95 1017.11 878.12 689.50 790.37 902.58 862.35
Standard Deviation 78.89 116.12 103.24 119.72 88.91 106.75 145.50 115.15 93.85 109.97 125.06 118.52
Intensity Change Log 2 — 0.364425 0.443667 0.389942 — 0.106948 0.486275 0.274290 — 0.233736 0.466400 0.329819

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001441 0.007895 0.015015 0.045326 0.001516 0.001086 0.031351 0.010993 0.001483 0.002295 0.018070 0.026958
Bud Neck 0.033235 0.011994 0.009024 0.017301 0.025471 0.013422 0.026153 0.027445 0.028943 0.013169 0.012227 0.022728
Bud Site 0.030997 0.045776 0.056461 0.227998* 0.027130 0.022886 0.139584* 0.109267* 0.028859 0.026949 0.072006 0.164478*
Cell Periphery 0.000795 0.000965 0.000467 0.001126 0.000649 0.000348 0.000363 0.000339 0.000715 0.000457 0.000447 0.000705
Cytoplasm 0.039948 0.014435 0.020870 0.066386 0.049521 0.021868 0.059537 0.080240 0.045241 0.020548 0.028101 0.073798
Cytoplasmic Foci 0.106819 0.061448 0.124520 0.104247 0.134398 0.098912 0.098196 0.147978 0.122066 0.092262 0.119597 0.127642
Eisosomes 0.000207 0.000165 0.000170 0.000350 0.000186 0.000120 0.000180 0.000160 0.000195 0.000128 0.000172 0.000248
Endoplasmic Reticulum 0.003177 0.001387 0.003464 0.008853 0.005417 0.001217 0.010568 0.003340 0.004416 0.001247 0.004793 0.005904
Endosome 0.080334 0.089563 0.131187 0.046063 0.077096 0.079365 0.044900 0.098447 0.078544 0.081175 0.115050 0.074088
Golgi 0.067092 0.100290 0.123078* 0.070708 0.068104 0.096492 0.049050 0.054858 0.067652 0.097166 0.109234 0.062229
Lipid Particles 0.011550 0.003645 0.004385 0.001856 0.014335 0.006354 0.004217 0.004140 0.013090 0.005873 0.004354 0.003078
Mitochondria 0.487783* 0.515888* 0.244188* 0.192305* 0.426630* 0.497372* 0.057809 0.130708* 0.453974* 0.500658* 0.209332* 0.159351*
Mitotic Spindle 0.007457 0.025932 0.081357 0.042291 0.014739 0.023226 0.185816* 0.069959 0.011483 0.023706 0.100893* 0.057093
None 0.001873 0.000928 0.000271 0.000294 0.001264 0.000504 0.000721 0.000304 0.001536 0.000579 0.000355 0.000299
Nuclear Periphery 0.000679 0.000108 0.001443 0.000306 0.000782 0.000267 0.002642 0.000837 0.000736 0.000239 0.001667 0.000590
Nuclear Periphery Foci 0.000916 0.000211 0.002187 0.000530 0.000496 0.000314 0.002225 0.001045 0.000684 0.000296 0.002195 0.000806
Nucleolus 0.002154 0.001414 0.001035 0.000407 0.001432 0.001173 0.002855 0.001599 0.001755 0.001216 0.001376 0.001044
Nucleus 0.019122 0.004799 0.005056 0.009173 0.026129 0.007574 0.036303 0.009980 0.022996 0.007081 0.010900 0.009605
Peroxisomes 0.050830 0.049335 0.062324 0.074552 0.033222 0.037084 0.159122* 0.057215 0.041095 0.039259 0.080427 0.065277
Vacuole 0.032739 0.038171 0.084560 0.074846 0.064751 0.059583 0.080619 0.165888 0.050437 0.055782 0.083823 0.123553
Vacuole Periphery 0.020851 0.025650 0.028937 0.015082 0.026732 0.030833 0.007792 0.025259 0.024102 0.029913 0.024982 0.020526

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.09 -8.92 -13.32 -8.19 -8.28 1.46 -6.98 -7.09 -7.49 4.35 -1.38 -11.20 -14.78 -13.63 -4.03
Bud Neck 5.33 7.43 4.42 -2.26 -6.39 4.83 -0.93 -0.69 -6.34 0.35 7.23 8.22 2.72 -6.14 -7.88
Bud Site -1.97 -6.99 -26.56 -18.12 -22.89* 1.37 -12.76 -15.57 -16.82 3.01 0.69 -13.20 -29.44 -28.86 -18.09
Cell Periphery -0.54 2.48 -1.82 -0.39 -4.44 1.68 1.67 1.83 0.45 0.60 2.07 2.16 0.29 -2.61 -2.75
Cytoplasm 4.85 4.64 -6.23 -11.23 -13.53 6.91 -2.51 -6.95 -14.15 -2.37 7.96 4.90 -9.27 -18.16 -15.31
Cytoplasmic Foci 6.38 -2.88 0.92 -6.29 4.65 5.60 6.03 -1.61 -7.76 -7.77 6.14 1.06 -0.56 -7.25 -1.80
Eisosomes 2.09 2.44 -6.22 -7.20 -8.27 3.98 0.35 1.60 -3.81 1.48 5.90 1.81 -3.47 -10.34 -5.64
Endoplasmic Reticulum 2.61 -0.50 -4.68 -6.64 -4.76 5.34 -3.16 2.29 -4.34 4.35 6.10 -0.99 -2.00 -8.10 -1.12
Endosome -1.04 -8.70 6.96 5.60 19.94 -0.46 7.08 -3.59 -3.36 -10.69 -0.64 -8.38 1.92 2.60 11.03
Golgi -3.51 -9.60 -0.05 3.57 10.26 -5.12 4.21 3.59 9.31 -1.80 -6.41 -8.88 2.46 8.98 11.82
Lipid Particles 4.71 4.98 7.31 1.60 4.03 4.73 5.90 6.38 2.14 0.56 5.87 7.76 9.43 3.56 2.20
Mitochondria -1.17 17.53 21.39 15.27 5.74 -5.00 30.91 25.97 34.02* -8.51 -3.94 24.94 33.18 35.81* 7.23
Mitotic Spindle -3.59 -17.62 -10.19 -2.92 7.27 -2.85 -12.32 -11.20 -9.17 7.95 -5.12 -20.23 -14.77 -9.49 8.42
None 1.22 3.07 3.05 1.04 -0.01 1.75 1.22 2.21 2.62 3.14 2.72 3.51 3.71 2.36 1.23
Nuclear Periphery 2.18 -1.94 0.95 -2.18 3.19 3.57 -1.47 -0.53 -5.11 1.40 3.50 -2.55 0.48 -4.57 2.90
Nuclear Periphery Foci 1.86 -2.39 0.70 -3.71 3.69 2.14 -4.55 -3.19 -4.12 3.07 2.16 -4.20 -1.07 -4.91 3.85
Nucleolus 0.53 2.48 4.13 1.31 3.34 0.56 -2.50 -0.59 -1.04 2.03 1.32 1.15 2.43 0.77 1.47
Nucleus 4.24 5.55 3.03 -2.16 -3.51 6.25 -3.59 5.03 -2.20 6.41 7.46 4.26 5.84 -2.83 1.38
Peroxisomes 0.21 -2.31 -3.92 -2.93 -1.93 -1.15 -9.92 -5.48 -4.35 7.65 0.48 -8.89 -6.65 -6.63 3.05
Vacuole -1.23 -13.11 -11.34 -6.71 -0.20 1.04 -5.10 -16.28 -17.39 -6.85 -1.61 -10.44 -19.13 -16.60 -9.09
Vacuole Periphery -1.33 -3.53 3.10 3.28 7.47 -1.72 9.82 1.26 2.96 -8.56 -3.01 -0.33 2.96 5.67 3.20
Standard name
Human Ortholog
Description Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
Localization
Cell Percentages mitochondrion (87%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mgr1

Mgr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mgr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available