Standard name | |
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Human Ortholog | |
Description | Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 16 | 29 | 35 | 24 | 39 | 51 | 59 | – | 38 | 42 | 48 | 2 | 6 | 5 | 20 | 11 | 12 | 3 | 5 | 13 | 2 | 10 | 2 |
Bud | 0 | 1 | 0 | 3 | 1 | 4 | 1 | 6 | – | 7 | 5 | 6 | 0 | 0 | 2 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 17 | 23 | 21 | 20 | 18 | 39 | 29 | – | 29 | 39 | 45 | 6 | 19 | 29 | 60 | 11 | 14 | 0 | 3 | 8 | 1 | 3 | 4 |
Cytoplasm | 29 | 10 | 22 | 14 | 20 | 29 | 36 | 40 | – | 41 | 25 | 25 | 3 | 10 | 20 | 42 | 30 | 17 | 5 | 2 | 1 | 0 | 1 | 1 |
Endoplasmic Reticulum | 253 | 227 | 274 | 186 | 130 | 305 | 413 | 333 | – | 186 | 226 | 314 | 34 | 94 | 148 | 4 | 3 | 0 | 267 | 238 | 280 | 97 | 162 | 172 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 14 | 1 | 45 | 77 | 56 | 23 | 27 | 15 | – | 3 | 2 | 12 | 1 | 1 | 0 | 11 | 23 | 30 | 1 | 1 | 4 | 1 | 2 | 2 |
Mitochondria | 5 | 2 | 18 | 66 | 22 | 87 | 125 | 103 | – | 128 | 105 | 171 | 0 | 0 | 0 | 16 | 22 | 17 | 3 | 0 | 2 | 0 | 2 | 3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 3 | 12 | 4 | 2 | 8 | 22 | 27 | – | 12 | 18 | 49 | 0 | 1 | 0 | 12 | 4 | 4 | 1 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | – | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 17 | 3 | 7 | 1 | 29 | 19 | 29 | 50 | – | 40 | 10 | 14 | 0 | 2 | 1 | 8 | 15 | 8 | 0 | 1 | 0 | 0 | 1 | 6 |
Unique Cell Count | 283 | 246 | 315 | 250 | 218 | 400 | 571 | 496 | 368 | 342 | 478 | 39 | 111 | 178 | 152 | 106 | 84 | 290 | 256 | 317 | 107 | 190 | 197 | |
Labelled Cell Count | 331 | 280 | 430 | 407 | 304 | 534 | 744 | 669 | 488 | 472 | 687 | 46 | 133 | 205 | 175 | 123 | 104 | 290 | 256 | 317 | 107 | 190 | 197 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.7 | 19.7 | 17.2 | 17.2 | 15.7 | 14.5 | 14.0 | 13.9 | – | 12.3 | 12.5 | 12.8 | 18.0 | 16.9 | 17.0 | 13.0 | 14.0 | 18.0 | 16.7 | 18.3 | 20.1 |
Std Deviation (1e-4) | 3.4 | 3.8 | 2.8 | 3.1 | 3.1 | 2.5 | 2.7 | 2.4 | – | 2.4 | 2.5 | 2.6 | 2.9 | 2.8 | 2.7 | 2.5 | 3.3 | 3.8 | 3.3 | 3.6 | 3.6 |
Intensity Change (Log2) | – | – | – | -0.0 | -0.13 | -0.25 | -0.3 | -0.31 | – | -0.49 | -0.47 | -0.43 | 0.06 | -0.03 | -0.02 | -0.41 | -0.3 | 0.07 | -0.04 | 0.09 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.8 | 0.7 | 0.2 | -0.1 | 1.2 | – | 0.5 | 1.3 | 0.4 | 0 | -1.3 | -2.7 | 1.3 | 0.4 | 1.4 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.5 | 0.8 | -1.6 | -0.3 | -0.8 | – | 0.3 | 1.8 | 1.0 | 0 | 3.0 | 3.1 | 8.5 | 1.0 | 2.6 |
Cytoplasm | -0.7 | 0.9 | 0.1 | -0.4 | 0.6 | – | 1.9 | 0.2 | -1.0 | 0 | 0.7 | 1.6 | 6.1 | 5.8 | 3.6 |
Endoplasmic Reticulum | -3.8 | -7.2 | -3.6 | -5.0 | -6.3 | – | -10.1 | -6.3 | -6.7 | 0 | -0.6 | -1.2 | -17.4 | -15.8 | -15.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 4.7 | 3.3 | -3.9 | -5.0 | -6.0 | – | -6.9 | -6.8 | -6.3 | -2.1 | -3.9 | -5.3 | -2.2 | 1.8 | 4.5 |
Mitochondria | 6.9 | 1.9 | 6.0 | 6.3 | 5.9 | – | 9.2 | 8.2 | 9.7 | 0 | 0 | -3.2 | 1.9 | 4.6 | 4.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.6 | -2.1 | -1.5 | 0 | 1.1 | – | -0.4 | 0.9 | 3.3 | 0 | 0 | 0 | 1.9 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 5.0 | 1.8 | 2.1 | 4.3 | – | 4.5 | 0.6 | 0.6 | 0 | 0 | 0 | 0 | 4.8 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 16.5364 | 16.3269 | 16.73 | 15.8496 | 16.4779 | 16.2703 | 17.2944 | 19.2993 | 16.6671 | 17.8599 | 15.6903 | 17.5498 | 20.5389 | 19.8318 | 18.8695 | 17.5437 | 18.1813 | 18.768 |
Actin | 0.0206 | 0.0006 | 0.0042 | 0.0015 | 0.0004 | 0.0008 | 0.0151 | 0.0006 | 0.0059 | 0.0002 | 0.0595 | 0.0016 | 0.0001 | 0.0001 | 0.0011 | 0.0127 | 0.0065 | 0.001 |
Bud | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 |
Bud Neck | 0.0003 | 0.0003 | 0.0004 | 0.0005 | 0.0011 | 0.0014 | 0.0004 | 0.0003 | 0.0002 | 0.0008 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0019 | 0.0003 |
Bud Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0 |
Bud Site | 0.0004 | 0.0007 | 0.0002 | 0 | 0 | 0 | 0.0007 | 0.0005 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0.0005 | 0.0006 | 0 | 0 |
Cell Periphery | 0.0025 | 0.0143 | 0.0031 | 0.0017 | 0.0029 | 0.001 | 0.001 | 0.0115 | 0.0017 | 0.0027 | 0.001 | 0.001 | 0.0007 | 0.01 | 0.0079 | 0.0032 | 0.0019 | 0.0012 |
Cytoplasm | 0.0231 | 0.035 | 0.0214 | 0.0103 | 0.0078 | 0.0125 | 0.0085 | 0.0442 | 0.0198 | 0.0124 | 0.0056 | 0.0011 | 0.0007 | 0.0091 | 0.0034 | 0.0042 | 0.0032 | 0.0011 |
Cytoplasmic Foci | 0.0074 | 0.0015 | 0.0012 | 0.0002 | 0.0042 | 0.0007 | 0.0104 | 0.0004 | 0.002 | 0.0006 | 0.0017 | 0.0002 | 0 | 0.0002 | 0.0158 | 0.0046 | 0.0006 | 0.0002 |
Eisosomes | 0.0002 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0.0004 | 0.0001 | 0 | 0.0014 | 0.0003 | 0.0001 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.8514 | 0.9026 | 0.9385 | 0.9653 | 0.9169 | 0.949 | 0.8985 | 0.905 | 0.9245 | 0.9616 | 0.8896 | 0.9795 | 0.9881 | 0.9481 | 0.9443 | 0.9341 | 0.911 | 0.958 |
Endosome | 0.0168 | 0.0032 | 0.0046 | 0.003 | 0.014 | 0.0033 | 0.0214 | 0.0022 | 0.0088 | 0.0016 | 0.0025 | 0.002 | 0.0002 | 0.0009 | 0.0011 | 0.0129 | 0.0121 | 0.004 |
Golgi | 0.0077 | 0.0023 | 0.0042 | 0.0017 | 0.006 | 0.002 | 0.0028 | 0.0018 | 0.0108 | 0.001 | 0.0269 | 0.0018 | 0.0001 | 0.0006 | 0.0014 | 0.0028 | 0.0079 | 0.0103 |
Lipid Particles | 0.0049 | 0.021 | 0.0061 | 0.0007 | 0.0292 | 0.0015 | 0.0053 | 0.0228 | 0.004 | 0.0123 | 0.0008 | 0.0013 | 0.0001 | 0.0034 | 0.0067 | 0.0032 | 0.0044 | 0.0052 |
Mitochondria | 0.0035 | 0.0001 | 0.0007 | 0.0004 | 0.0002 | 0.0001 | 0.0012 | 0.0002 | 0.0135 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0109 | 0.002 | 0.0039 | 0.0003 | 0.0001 |
None | 0.0025 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0042 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0022 | 0.0017 | 0.0007 | 0 |
Nuclear Periphery | 0.0425 | 0.0122 | 0.0115 | 0.0103 | 0.0092 | 0.0207 | 0.019 | 0.0034 | 0.0046 | 0.0045 | 0.008 | 0.008 | 0.0086 | 0.008 | 0.0069 | 0.0076 | 0.042 | 0.0151 |
Nucleolus | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 |
Nucleus | 0.0021 | 0.0008 | 0.0005 | 0.0008 | 0.0005 | 0.0033 | 0.0017 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0013 | 0.0023 | 0.0003 |
Peroxisomes | 0.0033 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0004 | 0 | 0.0012 | 0 | 0 | 0 | 0.0017 | 0.0025 | 0 | 0 |
Punctate Nuclear | 0.004 | 0.0006 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0042 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0006 | 0.002 | 0.0008 | 0 |
Vacuole | 0.004 | 0.0038 | 0.0021 | 0.0023 | 0.0059 | 0.0026 | 0.0021 | 0.0059 | 0.0022 | 0.0016 | 0.0011 | 0.0012 | 0.0009 | 0.0044 | 0.0019 | 0.0013 | 0.0024 | 0.002 |
Vacuole Periphery | 0.0024 | 0.0004 | 0.0005 | 0.0007 | 0.0009 | 0.0006 | 0.0013 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0003 | 0.002 | 0.0006 | 0.0006 | 0.0014 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 92.5545 | 68.2643 | 79.8298 | 102.1055 | 82.7161 | 69.5586 | 88.3525 | 90.8116 | 85.4176 | 105.9933 |
Translational Efficiency | 0.932 | 1.2585 | 1.0816 | 0.7334 | 1.0173 | 1.3252 | 1.0645 | 0.866 | 1.0406 | 0.9443 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
152 | 1962 | 212 | 996 | 2396 | 2015 | 1849 | 1720 | 2548 | 3977 | 2061 | 2716 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1589.65 | 1466.17 | 2232.14 | 1872.48 | 1373.40 | 1455.90 | 1893.57 | 1939.64 | 1386.30 | 1460.97 | 1928.40 | 1915.01 |
Standard Deviation | 223.45 | 277.83 | 347.45 | 367.49 | 238.31 | 240.79 | 308.50 | 321.34 | 242.91 | 259.77 | 329.21 | 340.54 |
Intensity Change Log 2 | — | -0.116657 | 0.489718 | 0.236241 | — | 0.084159 | 0.463357 | 0.498037 | — | -0.020092 | 0.477559 | 0.363511 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002791 | 0.000784 | 0.003556 | 0.002727 | 0.000434 | 0.001646 | 0.000436 | 0.003043 | 0.000574 | 0.001221 | 0.000757 | 0.002927 |
Bud Neck | 0.002945 | 0.000300 | 0.006646 | 0.003384 | 0.000066 | 0.000145 | 0.000332 | 0.001710 | 0.000237 | 0.000222 | 0.000982 | 0.002324 |
Bud Site | 0.011207 | 0.002017 | 0.002922 | 0.015577 | 0.001148 | 0.002489 | 0.001251 | 0.011075 | 0.001748 | 0.002256 | 0.001423 | 0.012726 |
Cell Periphery | 0.028033 | 0.002150 | 0.010366 | 0.056049 | 0.001989 | 0.002619 | 0.006920 | 0.023966 | 0.003543 | 0.002388 | 0.007275 | 0.035731 |
Cytoplasm | 0.009510 | 0.002217 | 0.001232 | 0.004558 | 0.001203 | 0.002831 | 0.001483 | 0.006240 | 0.001698 | 0.002528 | 0.001457 | 0.005623 |
Cytoplasmic Foci | 0.007974 | 0.004809 | 0.000316 | 0.002402 | 0.002474 | 0.003688 | 0.000364 | 0.002250 | 0.002803 | 0.004241 | 0.000359 | 0.002305 |
Eisosomes | 0.000429 | 0.000125 | 0.001106 | 0.001100 | 0.000073 | 0.000120 | 0.000105 | 0.000297 | 0.000094 | 0.000123 | 0.000208 | 0.000592 |
Endoplasmic Reticulum | 0.773947* | 0.960517* | 0.883371* | 0.780280* | 0.978790* | 0.964686* | 0.969249* | 0.885184* | 0.966570* | 0.962629* | 0.960415* | 0.846714* |
Endosome | 0.019893 | 0.008369 | 0.016320 | 0.012389 | 0.004283 | 0.006047 | 0.003999 | 0.007389 | 0.005215 | 0.007192 | 0.005267 | 0.009223 |
Golgi | 0.021935 | 0.004406 | 0.006492 | 0.019385 | 0.002736 | 0.004174 | 0.003101 | 0.008219 | 0.003881 | 0.004288 | 0.003450 | 0.012314 |
Lipid Particles | 0.056330 | 0.006372 | 0.020112 | 0.065480 | 0.002173 | 0.004121 | 0.005737 | 0.033384 | 0.005404 | 0.005231 | 0.007216 | 0.045154 |
Mitochondria | 0.003759 | 0.001118 | 0.000147 | 0.001689 | 0.000436 | 0.000952 | 0.000170 | 0.000842 | 0.000635 | 0.001034 | 0.000168 | 0.001153 |
Mitotic Spindle | 0.003381 | 0.000203 | 0.000370 | 0.000464 | 0.000080 | 0.000256 | 0.000050 | 0.000509 | 0.000277 | 0.000230 | 0.000083 | 0.000492 |
None | 0.000048 | 0.001262 | 0.000017 | 0.000235 | 0.001550 | 0.001935 | 0.000301 | 0.000333 | 0.001461 | 0.001603 | 0.000271 | 0.000297 |
Nuclear Periphery | 0.001069 | 0.000223 | 0.006190 | 0.001846 | 0.000148 | 0.000271 | 0.000516 | 0.001054 | 0.000203 | 0.000247 | 0.001099 | 0.001345 |
Nuclear Periphery Foci | 0.007514 | 0.002018 | 0.018716 | 0.005840 | 0.000630 | 0.001048 | 0.001500 | 0.002998 | 0.001041 | 0.001527 | 0.003271 | 0.004040 |
Nucleolus | 0.000305 | 0.000012 | 0.000016 | 0.000019 | 0.000002 | 0.000011 | 0.000005 | 0.000013 | 0.000020 | 0.000012 | 0.000006 | 0.000015 |
Nucleus | 0.000563 | 0.000059 | 0.000118 | 0.000368 | 0.000040 | 0.000086 | 0.000048 | 0.000285 | 0.000071 | 0.000073 | 0.000055 | 0.000316 |
Peroxisomes | 0.000388 | 0.000593 | 0.000011 | 0.000406 | 0.000768 | 0.000958 | 0.000154 | 0.000797 | 0.000745 | 0.000778 | 0.000139 | 0.000654 |
Vacuole | 0.033020 | 0.001712 | 0.020587 | 0.021205 | 0.000484 | 0.001148 | 0.003565 | 0.008432 | 0.002425 | 0.001426 | 0.005316 | 0.013116 |
Vacuole Periphery | 0.014960 | 0.000734 | 0.001389 | 0.004595 | 0.000491 | 0.000767 | 0.000713 | 0.001982 | 0.001354 | 0.000751 | 0.000782 | 0.002940 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.70 | -0.26 | -0.07 | -2.56 | 0.24 | -2.98 | -0.07 | -3.49 | -1.74 | -3.39 | -2.55 | -0.54 | -4.26 | -3.00 | -3.44 |
Bud Neck | 3.60 | -1.79 | -0.44 | -5.30 | 1.64 | -1.54 | -3.75 | -4.43 | -4.19 | -3.66 | 0.26 | -3.36 | -6.54 | -6.55 | -3.45 |
Bud Site | 3.43 | 2.89 | -1.26 | -5.93 | -5.12 | -2.00 | -0.21 | -6.46 | -5.48 | -6.32 | -0.95 | 0.51 | -8.35 | -8.09 | -8.54 |
Cell Periphery | 4.60 | 2.99 | -4.18 | -14.43 | -11.09 | -1.58 | -6.16 | -13.98 | -13.57 | -9.91 | 2.40 | -4.54 | -18.26 | -19.42 | -15.43 |
Cytoplasm | 1.68 | 1.83 | 1.12 | -2.90 | -3.01 | -4.07 | -0.69 | -4.79 | -3.25 | -4.19 | -2.08 | 0.35 | -5.06 | -4.24 | -4.98 |
Cytoplasmic Foci | 1.15 | 2.86 | 2.07 | 2.87 | -4.44 | -1.53 | 4.12 | 0.33 | 1.98 | -5.23 | -2.10 | 4.82 | 0.85 | 3.51 | -6.79 |
Eisosomes | 2.27 | -0.90 | -1.38 | -2.08 | -0.00 | -1.66 | -1.38 | -6.13 | -4.54 | -5.37 | -1.19 | -1.43 | -2.86 | -2.70 | -2.03 |
Endoplasmic Reticulum | -6.94 | -3.50 | -0.25 | 19.22 | 5.89 | 4.42 | 3.61 | 16.93 | 13.39 | 14.60 | 1.25 | 1.95 | 22.38 | 22.46 | 20.80 |
Endosome | 2.98 | 0.74 | 1.90 | -4.42 | 1.64 | -3.44 | 0.64 | -6.34 | -2.29 | -6.66 | -4.00 | -0.25 | -7.97 | -4.00 | -7.56 |
Golgi | 2.62 | 2.24 | 0.35 | -7.87 | -5.24 | -1.85 | -0.68 | -5.17 | -3.51 | -4.86 | -0.60 | 0.54 | -7.81 | -8.00 | -8.85 |
Lipid Particles | 3.97 | 2.53 | -0.60 | -12.80 | -6.10 | -2.80 | -4.32 | -12.85 | -11.70 | -10.87 | 0.24 | -1.60 | -16.34 | -17.06 | -15.35 |
Mitochondria | 1.11 | 1.55 | 0.83 | -0.61 | -1.82 | -2.26 | 3.48 | -2.68 | 0.37 | -4.87 | -1.45 | 2.98 | -1.48 | -0.32 | -3.07 |
Mitotic Spindle | 1.65 | 1.50 | 1.51 | -2.28 | -0.04 | -1.03 | 0.54 | -3.71 | -1.40 | -4.31 | 0.28 | 1.43 | -1.63 | -2.28 | -5.38 |
None | -4.97 | 1.54 | -3.74 | 4.11 | -4.58 | -0.54 | 2.85 | 2.79 | 2.81 | -0.35 | -0.28 | 2.89 | 2.85 | 4.15 | -0.35 |
Nuclear Periphery | 1.73 | -4.72 | -1.53 | -12.54 | 4.46 | -0.92 | -7.86 | -12.17 | -5.17 | -6.20 | -0.57 | -7.61 | -15.81 | -11.44 | -1.85 |
Nuclear Periphery Foci | 3.91 | -3.04 | 1.12 | -5.55 | 3.67 | -1.81 | -3.74 | -4.45 | -3.59 | -2.71 | -1.76 | -5.13 | -7.22 | -5.63 | -1.41 |
Nucleolus | 1.00 | 0.98 | 0.97 | -1.67 | -0.24 | -1.38 | -2.33 | -6.11 | -0.29 | -3.79 | 0.50 | 0.80 | 0.29 | -0.99 | -4.33 |
Nucleus | 2.21 | 1.87 | 0.74 | -2.51 | -1.85 | -1.44 | -1.30 | -5.17 | -3.74 | -4.98 | -0.00 | 0.86 | -4.50 | -4.46 | -4.85 |
Peroxisomes | -0.94 | 1.91 | -0.08 | 1.19 | -3.11 | -0.55 | 3.01 | -0.06 | 0.37 | -1.82 | -0.14 | 3.15 | 0.32 | 0.47 | -2.25 |
Vacuole | 4.19 | 1.43 | 1.52 | -9.50 | -0.10 | -2.26 | -6.56 | -8.86 | -7.83 | -4.93 | 1.95 | -3.82 | -10.23 | -12.16 | -6.98 |
Vacuole Periphery | 2.78 | 2.63 | 2.00 | -5.33 | -3.69 | -1.40 | -1.31 | -4.74 | -3.73 | -4.01 | 1.75 | 1.59 | -3.48 | -6.55 | -6.26 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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