Standard name
Human Ortholog
Description Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0.09 0.14 0.11 0.1 0.09 0.12 – 0.1 0.12 0.1 0.05 0.05 0 0.13 0.1 0.14 0 0 0 0 0.06 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.07 0.07 0.08 0.09 0 0.07 0.06 – 0.08 0.11 0.09 0.15 0.17 0.16 0.39 0.1 0.17 0 0 0 0 0 0
Cytoplasm 0.1 0 0.07 0.06 0.09 0.07 0.06 0.08 – 0.11 0.07 0.05 0.08 0.09 0.11 0.28 0.28 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0.89 0.92 0.87 0.74 0.6 0.76 0.72 0.67 – 0.51 0.66 0.66 0.87 0.85 0.83 0 0 0 0.92 0.93 0.89 0.91 0.85 0.88
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.05 0 0.14 0.31 0.26 0.06 0.05 0 – 0 0 0 0 0 0 0.07 0.22 0.36 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.26 0.1 0.22 0.22 0.21 – 0.35 0.31 0.36 0 0 0 0.11 0.21 0.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0.05 – 0 0.05 0.1 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0.13 0.05 0.05 0.1 – 0.11 0 0 0 0 0 0.05 0.14 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 16 29 35 24 39 51 59 – 38 42 48 2 6 5 20 11 12 3 5 13 2 10 2
Bud 0 1 0 3 1 4 1 6 – 7 5 6 0 0 2 1 3 0 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 17 23 21 20 18 39 29 – 29 39 45 6 19 29 60 11 14 0 3 8 1 3 4
Cytoplasm 29 10 22 14 20 29 36 40 – 41 25 25 3 10 20 42 30 17 5 2 1 0 1 1
Endoplasmic Reticulum 253 227 274 186 130 305 413 333 – 186 226 314 34 94 148 4 3 0 267 238 280 97 162 172
Endosome 0 0 0 0 0 1 0 0 – 2 0 0 0 0 0 0 1 2 0 0 0 0 0 0
Golgi 14 1 45 77 56 23 27 15 – 3 2 12 1 1 0 11 23 30 1 1 4 1 2 2
Mitochondria 5 2 18 66 22 87 125 103 – 128 105 171 0 0 0 16 22 17 3 0 2 0 2 3
Nucleus 0 0 0 0 0 1 0 2 – 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 2 3 12 4 2 8 22 27 – 12 18 49 0 1 0 12 4 4 1 0 1 0 1 0
Nucleolus 1 0 0 0 0 0 1 5 – 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 17 3 7 1 29 19 29 50 – 40 10 14 0 2 1 8 15 8 0 1 0 0 1 6
Unique Cell Count 283 246 315 250 218 400 571 496 368 342 478 39 111 178 152 106 84 290 256 317 107 190 197
Labelled Cell Count 331 280 430 407 304 534 744 669 488 472 687 46 133 205 175 123 104 290 256 317 107 190 197


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.7 19.7 17.2 17.2 15.7 14.5 14.0 13.9 – 12.3 12.5 12.8 18.0 16.9 17.0 13.0 14.0 18.0 16.7 18.3 20.1
Std Deviation (1e-4) 3.4 3.8 2.8 3.1 3.1 2.5 2.7 2.4 – 2.4 2.5 2.6 2.9 2.8 2.7 2.5 3.3 3.8 3.3 3.6 3.6
Intensity Change (Log2) – – – -0.0 -0.13 -0.25 -0.3 -0.31 – -0.49 -0.47 -0.43 0.06 -0.03 -0.02 -0.41 -0.3 0.07 -0.04 0.09 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.8 0.7 0.2 -0.1 1.2 – 0.5 1.3 0.4 0 -1.3 -2.7 1.3 0.4 1.4
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0.5 0.8 -1.6 -0.3 -0.8 – 0.3 1.8 1.0 0 3.0 3.1 8.5 1.0 2.6
Cytoplasm -0.7 0.9 0.1 -0.4 0.6 – 1.9 0.2 -1.0 0 0.7 1.6 6.1 5.8 3.6
Endoplasmic Reticulum -3.8 -7.2 -3.6 -5.0 -6.3 – -10.1 -6.3 -6.7 0 -0.6 -1.2 -17.4 -15.8 -15.3
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 4.7 3.3 -3.9 -5.0 -6.0 – -6.9 -6.8 -6.3 -2.1 -3.9 -5.3 -2.2 1.8 4.5
Mitochondria 6.9 1.9 6.0 6.3 5.9 – 9.2 8.2 9.7 0 0 -3.2 1.9 4.6 4.2
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.6 -2.1 -1.5 0 1.1 – -0.4 0.9 3.3 0 0 0 1.9 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 5.0 1.8 2.1 4.3 – 4.5 0.6 0.6 0 0 0 0 4.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 16.5364 16.3269 16.73 15.8496 16.4779 16.2703 17.2944 19.2993 16.6671 17.8599 15.6903 17.5498 20.5389 19.8318 18.8695 17.5437 18.1813 18.768
Actin 0.0206 0.0006 0.0042 0.0015 0.0004 0.0008 0.0151 0.0006 0.0059 0.0002 0.0595 0.0016 0.0001 0.0001 0.0011 0.0127 0.0065 0.001
Bud 0.0001 0 0.0001 0.0001 0 0 0.0003 0 0 0 0.0001 0 0 0 0.0001 0.0002 0 0
Bud Neck 0.0003 0.0003 0.0004 0.0005 0.0011 0.0014 0.0004 0.0003 0.0002 0.0008 0.0003 0.0002 0 0.0001 0.0003 0.0002 0.0019 0.0003
Bud Periphery 0.0002 0.0001 0.0001 0.0004 0.0001 0.0001 0.0009 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0002 0.0007 0.0003 0.0001 0
Bud Site 0.0004 0.0007 0.0002 0 0 0 0.0007 0.0005 0.0001 0 0.0004 0 0 0 0.0005 0.0006 0 0
Cell Periphery 0.0025 0.0143 0.0031 0.0017 0.0029 0.001 0.001 0.0115 0.0017 0.0027 0.001 0.001 0.0007 0.01 0.0079 0.0032 0.0019 0.0012
Cytoplasm 0.0231 0.035 0.0214 0.0103 0.0078 0.0125 0.0085 0.0442 0.0198 0.0124 0.0056 0.0011 0.0007 0.0091 0.0034 0.0042 0.0032 0.0011
Cytoplasmic Foci 0.0074 0.0015 0.0012 0.0002 0.0042 0.0007 0.0104 0.0004 0.002 0.0006 0.0017 0.0002 0 0.0002 0.0158 0.0046 0.0006 0.0002
Eisosomes 0.0002 0.0003 0.0001 0 0 0 0.0002 0.0002 0 0 0.0004 0.0001 0 0.0014 0.0003 0.0001 0.0004 0.0001
Endoplasmic Reticulum 0.8514 0.9026 0.9385 0.9653 0.9169 0.949 0.8985 0.905 0.9245 0.9616 0.8896 0.9795 0.9881 0.9481 0.9443 0.9341 0.911 0.958
Endosome 0.0168 0.0032 0.0046 0.003 0.014 0.0033 0.0214 0.0022 0.0088 0.0016 0.0025 0.002 0.0002 0.0009 0.0011 0.0129 0.0121 0.004
Golgi 0.0077 0.0023 0.0042 0.0017 0.006 0.002 0.0028 0.0018 0.0108 0.001 0.0269 0.0018 0.0001 0.0006 0.0014 0.0028 0.0079 0.0103
Lipid Particles 0.0049 0.021 0.0061 0.0007 0.0292 0.0015 0.0053 0.0228 0.004 0.0123 0.0008 0.0013 0.0001 0.0034 0.0067 0.0032 0.0044 0.0052
Mitochondria 0.0035 0.0001 0.0007 0.0004 0.0002 0.0001 0.0012 0.0002 0.0135 0.0001 0.0001 0.0008 0 0.0109 0.002 0.0039 0.0003 0.0001
None 0.0025 0.0001 0.0001 0.0001 0.0001 0.0001 0.0042 0.0001 0.0003 0 0.0001 0.0001 0 0.0001 0.0022 0.0017 0.0007 0
Nuclear Periphery 0.0425 0.0122 0.0115 0.0103 0.0092 0.0207 0.019 0.0034 0.0046 0.0045 0.008 0.008 0.0086 0.008 0.0069 0.0076 0.042 0.0151
Nucleolus 0.0002 0 0 0 0 0 0.0002 0.0001 0 0 0 0 0 0 0.0002 0.0001 0.0002 0
Nucleus 0.0021 0.0008 0.0005 0.0008 0.0005 0.0033 0.0017 0.0002 0.0002 0.0002 0.0003 0.0003 0.0001 0.0002 0.0002 0.0013 0.0023 0.0003
Peroxisomes 0.0033 0 0.0001 0 0.0001 0 0.0005 0 0.0004 0 0.0012 0 0 0 0.0017 0.0025 0 0
Punctate Nuclear 0.004 0.0006 0.0001 0 0.0003 0.0001 0.0042 0 0.0001 0 0 0.0001 0 0 0.0006 0.002 0.0008 0
Vacuole 0.004 0.0038 0.0021 0.0023 0.0059 0.0026 0.0021 0.0059 0.0022 0.0016 0.0011 0.0012 0.0009 0.0044 0.0019 0.0013 0.0024 0.002
Vacuole Periphery 0.0024 0.0004 0.0005 0.0007 0.0009 0.0006 0.0013 0.0004 0.0005 0.0002 0.0003 0.0005 0.0003 0.002 0.0006 0.0006 0.0014 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 92.5545 68.2643 79.8298 102.1055 82.7161 69.5586 88.3525 90.8116 85.4176 105.9933
Translational Efficiency 0.932 1.2585 1.0816 0.7334 1.0173 1.3252 1.0645 0.866 1.0406 0.9443

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Emc1

Emc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Emc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available