Standard name | |
---|---|
Human Ortholog | |
Description | Amphiphysin-like lipid raft protein; N-BAR domain protein that interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 58 | 46 | 147 | 119 | 120 | 158 | 92 | 161 | 171 | 168 | 138 | 193 | 24 | 25 | 67 | 37 | 4 | 7 | 121 | 96 | 242 | 16 | 17 | 9 |
Bud | 20 | 16 | 37 | 24 | 41 | 46 | 34 | 33 | 50 | 40 | 40 | 50 | 3 | 6 | 19 | 3 | 0 | 1 | 6 | 5 | 7 | 2 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 5 | 1 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 2 | 1 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 22 | 18 | 46 | 41 | 25 | 36 | 18 | 41 | 23 | 34 | 43 | 39 | 18 | 21 | 35 | 17 | 5 | 6 | 7 | 2 | 10 | 1 | 2 | 0 |
Cytoplasm | 60 | 63 | 114 | 97 | 122 | 91 | 74 | 64 | 74 | 65 | 51 | 48 | 53 | 136 | 132 | 52 | 11 | 14 | 38 | 44 | 81 | 62 | 124 | 144 |
Endoplasmic Reticulum | 25 | 27 | 62 | 72 | 73 | 91 | 54 | 75 | 77 | 72 | 65 | 49 | 20 | 28 | 53 | 5 | 1 | 4 | 4 | 9 | 22 | 5 | 7 | 3 |
Endosome | 0 | 1 | 1 | 0 | 3 | 3 | 4 | 4 | 3 | 7 | 3 | 13 | 0 | 0 | 3 | 0 | 1 | 0 | 4 | 6 | 11 | 1 | 1 | 1 |
Golgi | 16 | 7 | 87 | 64 | 97 | 72 | 30 | 166 | 82 | 145 | 93 | 112 | 7 | 10 | 39 | 22 | 3 | 15 | 2 | 5 | 6 | 2 | 2 | 1 |
Mitochondria | 2 | 3 | 0 | 1 | 2 | 8 | 55 | 13 | 96 | 48 | 74 | 80 | 0 | 0 | 0 | 2 | 2 | 3 | 3 | 2 | 1 | 1 | 0 | 0 |
Nucleus | 0 | 1 | 2 | 1 | 0 | 0 | 3 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 |
Nucleolus | 2 | 0 | 0 | 3 | 4 | 2 | 1 | 7 | 3 | 9 | 1 | 7 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 1 | 0 | 2 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 9 | 0 | 2 | 2 | 1 | 4 | 0 | 0 | 3 | 5 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 1 | 3 | 1 | 1 | 6 | 13 | 17 | 25 | 9 | 18 | 13 | 8 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 7 | 1 | 2 | 2 | 3 |
Unique Cell Count | 157 | 150 | 343 | 301 | 361 | 346 | 271 | 409 | 383 | 402 | 319 | 381 | 97 | 192 | 252 | 111 | 18 | 36 | 197 | 190 | 400 | 102 | 170 | 176 |
Labelled Cell Count | 206 | 185 | 507 | 425 | 496 | 525 | 384 | 598 | 591 | 610 | 527 | 622 | 128 | 227 | 349 | 140 | 27 | 51 | 197 | 190 | 400 | 102 | 170 | 176 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 23.1 | 19.1 | 21.3 | 20.1 | 20.2 | 18.4 | 11.9 | 17.6 | 15.7 | 16.7 | 15.7 | 15.6 | 21.1 | 20.6 | 21.2 | 20.0 | 25.8 | 27.0 | 27.3 | 34.6 | 39.0 |
Std Deviation (1e-4) | 5.5 | 4.5 | 3.3 | 3.5 | 3.7 | 3.6 | 3.8 | 3.6 | 3.6 | 4.1 | 3.6 | 3.7 | 4.2 | 4.7 | 4.5 | 4.9 | 6.3 | 7.0 | 7.8 | 9.8 | 13.6 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.07 | -0.21 | -0.84 | -0.27 | -0.43 | -0.35 | -0.44 | -0.45 | -0.01 | -0.04 | -0.0 | -0.09 | 0.28 | 0.34 | 0.36 | 0.7 | 0.87 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.9 | -2.6 | 0.7 | -2.2 | -1.0 | 0.5 | -0.3 | 0.1 | 2.1 | -3.2 | -7.1 | -4.1 | -1.8 | -1.7 | -2.7 |
Bud | -1.2 | 0.2 | 1.0 | 0.7 | -1.3 | 0.9 | -0.4 | 0.7 | 1.0 | -2.3 | -3.1 | -1.3 | -2.6 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.1 | -2.9 | -1.2 | -2.7 | -1.4 | -3.4 | -2.2 | 0 | -1.3 | 1.3 | -0.8 | 0.2 | 0.5 | 0 | 0 |
Cytoplasm | -0.3 | 0.2 | -2.0 | -1.6 | -5.7 | -4.3 | -5.4 | -5.1 | -6.7 | 3.8 | 8.4 | 4.7 | 2.6 | 2.4 | 0.7 |
Endoplasmic Reticulum | 1.8 | 0.7 | 2.6 | 0.6 | 0.1 | 0.7 | -0.1 | 0.8 | -1.9 | 0.6 | -1.0 | 0.9 | -3.5 | 0 | -1.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -1.2 | 0.5 | -1.4 | -4.5 | 4.4 | -1.3 | 3.1 | 1.1 | 1.2 | -3.9 | -5.8 | -2.9 | -1.2 | 0 | 2.1 |
Mitochondria | 0 | 0 | 0 | 8.7 | 3.3 | 10.0 | 6.6 | 9.5 | 9.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | -2.2 | -2.1 | 0 | -1.7 | 0 | 0 | -1.6 | -1.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 3.2 | 4.4 | 4.4 | 0 | 3.6 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 17.6395 | 18.2186 | 16.8556 | 18.8189 | 14.1649 | 17.9445 | 22.7912 | 22.2907 | 21.8385 | 21.0018 | 20.0175 | 21.819 | 16.969 | 20.0368 | 18.2771 | 17.9978 | 16.4228 | 19.6176 |
Actin | 0.8095 | 0.7601 | 0.816 | 0.952 | 0.7621 | 0.8817 | 0.8158 | 0.8563 | 0.9213 | 0.7339 | 0.8714 | 0.9502 | 0.6694 | 0.6957 | 0.7678 | 0.7883 | 0.7196 | 0.8705 |
Bud | 0.0045 | 0.0066 | 0.0076 | 0.0129 | 0.0045 | 0.0011 | 0.0009 | 0.0008 | 0.018 | 0.0475 | 0.0044 | 0.0005 | 0.0024 | 0.0037 | 0.025 | 0.0236 | 0.0042 | 0.001 |
Bud Neck | 0.0055 | 0.003 | 0.0149 | 0.0031 | 0.0017 | 0.0065 | 0.001 | 0.0009 | 0.0045 | 0.0101 | 0.0005 | 0.0256 | 0.0068 | 0.0022 | 0.0045 | 0.0046 | 0.0034 | 0.0372 |
Bud Periphery | 0.0034 | 0.0038 | 0.0087 | 0.0212 | 0.0098 | 0.0011 | 0.0005 | 0.0004 | 0.0187 | 0.0677 | 0.0035 | 0.0004 | 0.0021 | 0.0017 | 0.0142 | 0.0482 | 0.005 | 0.0011 |
Bud Site | 0.0112 | 0.0825 | 0.0088 | 0.0014 | 0.0151 | 0.002 | 0.0149 | 0.0391 | 0.0042 | 0.0029 | 0.002 | 0.0006 | 0.0202 | 0.0798 | 0.0174 | 0.0109 | 0.0038 | 0.0014 |
Cell Periphery | 0.0033 | 0.0016 | 0.0007 | 0.0004 | 0.0035 | 0.0002 | 0.0005 | 0.0004 | 0.0004 | 0.001 | 0.0002 | 0.0001 | 0.0006 | 0.0006 | 0.0014 | 0.0036 | 0.0004 | 0.0002 |
Cytoplasm | 0.0228 | 0.0945 | 0.0443 | 0.0009 | 0.0046 | 0.029 | 0.0681 | 0.0737 | 0.0215 | 0.1007 | 0.0134 | 0.0103 | 0.0812 | 0.1386 | 0.0665 | 0.0182 | 0.0792 | 0.0301 |
Cytoplasmic Foci | 0.0267 | 0.0204 | 0.0206 | 0.0005 | 0.0173 | 0.0071 | 0.0253 | 0.0119 | 0.003 | 0.0057 | 0.0113 | 0.0046 | 0.0502 | 0.041 | 0.0186 | 0.0146 | 0.0225 | 0.0154 |
Eisosomes | 0.0024 | 0.0007 | 0.0005 | 0.0043 | 0.0013 | 0.001 | 0.0011 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.0007 | 0.0003 | 0.0015 | 0.0005 | 0.0006 | 0.0005 |
Endoplasmic Reticulum | 0.0046 | 0.0006 | 0.0019 | 0.0001 | 0.0075 | 0.0031 | 0.0056 | 0.0005 | 0.0002 | 0.003 | 0.0005 | 0.0001 | 0.0036 | 0.002 | 0.0058 | 0.0091 | 0.0125 | 0.001 |
Endosome | 0.024 | 0.0024 | 0.0145 | 0.0002 | 0.0398 | 0.0133 | 0.028 | 0.0012 | 0.0005 | 0.0059 | 0.0242 | 0.0004 | 0.0345 | 0.0034 | 0.0246 | 0.0144 | 0.0353 | 0.0052 |
Golgi | 0.0335 | 0.0121 | 0.0268 | 0.0023 | 0.0569 | 0.0446 | 0.0267 | 0.0086 | 0.0056 | 0.0147 | 0.0573 | 0.0044 | 0.0342 | 0.0147 | 0.0259 | 0.02 | 0.0404 | 0.0246 |
Lipid Particles | 0.0117 | 0.0013 | 0.0096 | 0.0001 | 0.0353 | 0.0013 | 0.0014 | 0.0009 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0138 | 0.0044 | 0.0071 | 0.0086 | 0.0312 | 0.0007 |
Mitochondria | 0.0074 | 0.0005 | 0.0116 | 0.0002 | 0.0236 | 0.0053 | 0.0018 | 0.0004 | 0.0001 | 0.0027 | 0.0075 | 0.0002 | 0.0086 | 0.0005 | 0.0016 | 0.02 | 0.0139 | 0.0073 |
None | 0.0031 | 0.0001 | 0.0006 | 0 | 0.0012 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0004 | 0.0002 | 0 | 0.0019 | 0.0004 | 0.0073 | 0.0018 | 0.0013 | 0.0001 |
Nuclear Periphery | 0.0031 | 0.0001 | 0.0019 | 0 | 0.0028 | 0.0005 | 0.0012 | 0.0001 | 0 | 0.0006 | 0.0001 | 0 | 0.0143 | 0.0002 | 0.0016 | 0.0018 | 0.0097 | 0.0001 |
Nucleolus | 0.0016 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0021 | 0 | 0.0002 | 0.0005 | 0.0004 | 0 |
Nucleus | 0.0018 | 0.0001 | 0.0005 | 0 | 0.0009 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0302 | 0.0002 | 0.0007 | 0.0008 | 0.0008 | 0.0001 |
Peroxisomes | 0.007 | 0.0092 | 0.0072 | 0.0003 | 0.0045 | 0.0012 | 0.004 | 0.004 | 0.0016 | 0.001 | 0.0019 | 0.002 | 0.017 | 0.01 | 0.0029 | 0.0039 | 0.0077 | 0.0031 |
Punctate Nuclear | 0.0095 | 0.0001 | 0.0009 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0026 | 0.0005 | 0.0028 | 0.0006 | 0.0063 | 0.0001 |
Vacuole | 0.0023 | 0.0003 | 0.0014 | 0.0001 | 0.0046 | 0.0003 | 0.0015 | 0.0001 | 0.0001 | 0.0009 | 0.0003 | 0 | 0.0025 | 0.0001 | 0.0022 | 0.0046 | 0.0008 | 0.0002 |
Vacuole Periphery | 0.0013 | 0 | 0.0008 | 0 | 0.0019 | 0.0004 | 0.0009 | 0 | 0 | 0.0004 | 0.0004 | 0 | 0.0011 | 0 | 0.0004 | 0.0014 | 0.0009 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 90.5677 | 48.2064 | 42.1917 | 69.7521 | 74.399 | 96.7363 | 71.5499 | 66.2161 | 73.5316 | 81.6717 |
Translational Efficiency | 1.4972 | 2.0847 | 2.0865 | 1.4184 | 1.4928 | 1.5154 | 1.5397 | 1.7642 | 1.7373 | 1.6839 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
29 | 645 | 365 | 1109 | 1589 | 2179 | 1330 | 1482 | 1618 | 2824 | 1695 | 2591 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1685.67 | 1506.55 | 2046.65 | 2082.40 | 1529.06 | 1764.48 | 2104.85 | 2344.81 | 1531.87 | 1705.57 | 2092.32 | 2232.49 |
Standard Deviation | 687.96 | 305.42 | 349.53 | 510.03 | 291.22 | 298.78 | 415.93 | 477.60 | 303.65 | 319.23 | 403.27 | 508.59 |
Intensity Change Log 2 | — | -0.162074 | 0.279942 | 0.304925 | — | 0.206598 | 0.461072 | 0.616826 | — | 0.025047 | 0.368935 | 0.461700 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.046133 | 0.727798* | 0.643661* | 0.613230* | 0.836065* | 0.810809* | 0.656165* | 0.598678* | 0.821907* | 0.791849* | 0.653472* | 0.604906* |
Bud Neck | 0.010480 | 0.006651 | 0.032822 | 0.012873 | 0.007534 | 0.003474 | 0.015215 | 0.015545 | 0.007587 | 0.004200 | 0.019006 | 0.014401 |
Bud Site | 0.108242* | 0.061788 | 0.165666* | 0.156877* | 0.071928 | 0.097196 | 0.124802* | 0.208942* | 0.072579 | 0.089108 | 0.133602* | 0.186657* |
Cell Periphery | 0.079783 | 0.001282 | 0.020389 | 0.042036 | 0.003173 | 0.001611 | 0.016043 | 0.036281 | 0.004546 | 0.001536 | 0.016979 | 0.038744 |
Cytoplasm | 0.143796* | 0.022460 | 0.009956 | 0.034157 | 0.013717 | 0.028251 | 0.022287 | 0.049191 | 0.016049 | 0.026928 | 0.019631 | 0.042756 |
Cytoplasmic Foci | 0.031722 | 0.031024 | 0.010349 | 0.007628 | 0.012538 | 0.019038 | 0.015337 | 0.006077 | 0.012882 | 0.021776 | 0.014263 | 0.006741 |
Eisosomes | 0.000678 | 0.001859 | 0.010492 | 0.019932 | 0.004312 | 0.001169 | 0.008916 | 0.012249 | 0.004247 | 0.001327 | 0.009255 | 0.015537 |
Endoplasmic Reticulum | 0.027605 | 0.002755 | 0.010027 | 0.003568 | 0.004841 | 0.000820 | 0.005714 | 0.002960 | 0.005249 | 0.001262 | 0.006643 | 0.003220 |
Endosome | 0.025083 | 0.042527 | 0.019183 | 0.011990 | 0.011792 | 0.006281 | 0.026296 | 0.005111 | 0.012031 | 0.014559 | 0.024764 | 0.008055 |
Golgi | 0.125547* | 0.032127 | 0.013619 | 0.017149 | 0.015019 | 0.010400 | 0.026466 | 0.011694 | 0.017000 | 0.015363 | 0.023699 | 0.014029 |
Lipid Particles | 0.025092 | 0.046488 | 0.049779 | 0.044548 | 0.011634 | 0.003932 | 0.065880 | 0.038969 | 0.011876 | 0.013651 | 0.062413 | 0.041357 |
Mitochondria | 0.017511 | 0.000372 | 0.000190 | 0.006564 | 0.000625 | 0.000543 | 0.000322 | 0.001036 | 0.000928 | 0.000504 | 0.000293 | 0.003402 |
Mitotic Spindle | 0.011016 | 0.001897 | 0.000909 | 0.004433 | 0.000602 | 0.001049 | 0.001101 | 0.000938 | 0.000789 | 0.001243 | 0.001060 | 0.002434 |
None | 0.002491 | 0.000054 | 0.000041 | 0.000144 | 0.000056 | 0.000082 | 0.000079 | 0.000214 | 0.000099 | 0.000076 | 0.000070 | 0.000184 |
Nuclear Periphery | 0.001412 | 0.000017 | 0.000098 | 0.000270 | 0.000009 | 0.000003 | 0.000070 | 0.000093 | 0.000035 | 0.000006 | 0.000076 | 0.000169 |
Nuclear Periphery Foci | 0.049760 | 0.000319 | 0.000551 | 0.001148 | 0.000173 | 0.000044 | 0.000574 | 0.000322 | 0.001062 | 0.000106 | 0.000569 | 0.000675 |
Nucleolus | 0.036380 | 0.000014 | 0.000007 | 0.000413 | 0.000005 | 0.000004 | 0.000006 | 0.000018 | 0.000657 | 0.000006 | 0.000006 | 0.000187 |
Nucleus | 0.185652* | 0.000140 | 0.000289 | 0.000872 | 0.000152 | 0.000089 | 0.000396 | 0.000731 | 0.003477 | 0.000101 | 0.000373 | 0.000791 |
Peroxisomes | 0.005652 | 0.016846 | 0.001617 | 0.002976 | 0.005002 | 0.014443 | 0.004312 | 0.003095 | 0.005014 | 0.014992 | 0.003732 | 0.003044 |
Vacuole | 0.045979 | 0.002240 | 0.009871 | 0.014896 | 0.000608 | 0.000534 | 0.009559 | 0.006682 | 0.001421 | 0.000924 | 0.009626 | 0.010198 |
Vacuole Periphery | 0.019986 | 0.001342 | 0.000485 | 0.004297 | 0.000212 | 0.000228 | 0.000463 | 0.001174 | 0.000566 | 0.000482 | 0.000467 | 0.002511 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -25.62* | -20.09 | -22.19 | 6.86 | 1.25 | 2.68 | 16.05 | 20.75* | 19.00* | 3.81 | 3.23 | 15.92 | 21.79* | 20.73* | 3.87 |
Bud Neck | 0.95 | -4.19 | -0.48 | -5.47 | 6.17 | 4.44 | -6.14 | -6.51 | -12.54 | -0.22 | 3.79 | -8.75 | -6.57 | -14.59 | 3.93 |
Bud Site | 1.59 | -2.23 | -2.01 | -11.32 | 0.73 | -4.23 | -8.26 | -17.61 | -14.24 | -9.51 | -3.08 | -10.02 | -18.36 | -16.61 | -7.41 |
Cell Periphery | 2.20 | 1.64 | 1.02 | -12.57 | -4.94 | 3.17 | -7.74 | -12.92 | -13.62 | -6.83 | 3.73 | -7.75 | -15.97 | -18.45 | -8.94 |
Cytoplasm | 2.61 | 2.88 | 2.33 | -2.60 | -5.90 | -5.04 | -3.72 | -8.99 | -5.19 | -5.27 | -3.94 | -2.27 | -8.68 | -5.39 | -6.23 |
Cytoplasmic Foci | -0.04 | 2.05 | 2.60 | 7.12 | 1.47 | -3.07 | -1.56 | 4.41 | 7.50 | 5.94 | -4.55 | -1.04 | 4.38 | 9.87 | 5.80 |
Eisosomes | -2.06 | -4.75 | -9.08 | -8.45 | -3.26 | 4.89 | -3.23 | -4.89 | -7.44 | -1.79 | 4.59 | -3.97 | -8.11 | -11.43 | -3.92 |
Endoplasmic Reticulum | 3.48 | 2.37 | 3.37 | -1.15 | 3.06 | 5.45 | -0.91 | 2.45 | -7.30 | 4.19 | 5.33 | -1.40 | 2.64 | -6.78 | 4.95 |
Endosome | -2.67 | 0.82 | 2.26 | 6.91 | 1.88 | 4.10 | -6.50 | 5.16 | 1.27 | 10.60 | -1.57 | -6.28 | 2.96 | 4.92 | 9.20 |
Golgi | 2.52 | 3.05 | 2.95 | 3.57 | -1.01 | 2.47 | -4.14 | 1.45 | -0.60 | 5.10 | 0.77 | -2.70 | 1.34 | 0.74 | 4.11 |
Lipid Particles | -2.55 | -2.82 | -2.60 | 0.27 | 0.83 | 5.09 | -12.61 | -8.40 | -11.65 | 5.46 | -0.95 | -13.61 | -11.13 | -10.57 | 5.19 |
Mitochondria | 1.17 | 1.18 | 0.73 | -4.60 | -4.75 | 0.44 | 2.08 | -1.44 | -1.75 | -2.77 | 1.35 | 2.15 | -3.76 | -4.81 | -5.23 |
Mitotic Spindle | 1.35 | 1.42 | 0.98 | -2.01 | -2.40 | -1.05 | -1.53 | -0.89 | 0.32 | 0.56 | -1.03 | -0.99 | -2.95 | -2.10 | -2.46 |
None | 3.86 | 3.88 | 3.71 | -3.66 | -4.32 | -0.43 | -0.79 | -3.32 | -1.98 | -2.64 | 0.64 | 1.20 | -2.48 | -2.30 | -3.38 |
Nuclear Periphery | 2.59 | 2.44 | 2.12 | -4.03 | -2.64 | 3.14 | -10.62 | -9.89 | -11.18 | -2.26 | 2.69 | -3.42 | -4.51 | -5.97 | -3.28 |
Nuclear Periphery Foci | 2.00 | 1.98 | 1.97 | -2.13 | -1.10 | 2.23 | -3.57 | -1.11 | -5.28 | 3.08 | 2.11 | 1.00 | 0.90 | -3.40 | -0.18 |
Nucleolus | 2.59 | 2.59 | 2.56 | -1.26 | -1.29 | 0.63 | -0.09 | -1.90 | -2.58 | -2.20 | 2.37 | 2.37 | 1.63 | -1.34 | -1.34 |
Nucleus | 3.58 | 3.57 | 3.56 | -2.61 | -1.92 | 0.99 | -2.67 | -5.32 | -8.74 | -1.73 | 3.08 | 2.71 | 2.41 | -5.56 | -2.06 |
Peroxisomes | -3.08 | 1.48 | 0.99 | 6.06 | -3.84 | -7.83 | 0.99 | 2.77 | 9.69 | 1.76 | -8.96 | 1.99 | 3.33 | 11.67 | 1.36 |
Vacuole | 3.22 | 2.62 | 2.28 | -6.44 | -1.87 | 0.43 | -8.86 | -8.12 | -8.21 | 2.77 | 1.52 | -9.13 | -9.09 | -10.00 | 0.18 |
Vacuole Periphery | 4.14 | 4.43 | 3.50 | -2.43 | -4.92 | -0.23 | -4.20 | -3.50 | -3.40 | -2.54 | 0.32 | 0.70 | -5.15 | -4.78 | -5.54 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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