ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Bud | 0 | 1 | 0 | 0 | 2 | 2 | 4 | 1 | 8 | 7 | 2 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 3 | 2 | 4 | 8 | 9 |
Bud Neck | 3 | 1 | 5 | 3 | 11 | 3 | 3 | 3 | 2 | 1 | 2 | 5 | 0 | 8 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 |
Bud Site | 0 | 3 | 1 | 4 | 9 | 8 | 8 | 1 | 22 | 14 | 9 | 28 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Cytoplasm | 4 | 5 | 10 | 5 | 3 | 9 | 17 | 22 | 9 | 8 | 4 | 6 | 11 | 28 | 38 | 17 | 18 | 15 | 0 | 0 | 2 | 31 | 32 | 25 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 2 | 5 | 2 |
Endosome | 5 | 11 | 9 | 8 | 4 | 29 | 30 | 32 | 9 | 21 | 11 | 8 | 11 | 11 | 26 | 6 | 3 | 1 | 6 | 9 | 12 | 9 | 9 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 5 | 3 | 0 | 1 | 2 |
Mitochondria | 20 | 22 | 5 | 6 | 35 | 22 | 30 | 22 | 115 | 76 | 78 | 104 | 1 | 2 | 1 | 29 | 6 | 10 | 2 | 7 | 0 | 10 | 25 | 9 |
Nucleus | 121 | 107 | 48 | 22 | 22 | 38 | 42 | 55 | 58 | 68 | 54 | 85 | 23 | 21 | 25 | 69 | 26 | 29 | 76 | 69 | 40 | 19 | 13 | 11 |
Nuclear Periphery | 2 | 6 | 4 | 0 | 1 | 2 | 2 | 2 | 0 | 12 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 7 |
Nucleolus | 352 | 403 | 228 | 172 | 167 | 282 | 360 | 277 | 374 | 349 | 275 | 342 | 141 | 199 | 236 | 197 | 115 | 115 | 335 | 379 | 189 | 134 | 138 | 172 |
Peroxisomes | 0 | 3 | 2 | 0 | 0 | 4 | 3 | 2 | 5 | 4 | 1 | 1 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 21 | 56 | 76 | 71 | 57 | 145 | 197 | 162 | 179 | 200 | 94 | 135 | 48 | 93 | 104 | 17 | 10 | 15 | 20 | 36 | 37 | 30 | 46 | 40 |
Vac/Vac Membrane | 4 | 7 | 1 | 2 | 0 | 3 | 0 | 2 | 2 | 0 | 1 | 2 | 1 | 3 | 3 | 8 | 3 | 3 | 6 | 6 | 8 | 3 | 8 | 7 |
Unique Cell Count | 436 | 491 | 289 | 203 | 204 | 369 | 453 | 391 | 502 | 484 | 355 | 492 | 168 | 253 | 297 | 268 | 158 | 155 | 461 | 523 | 301 | 256 | 307 | 313 |
Labelled Cell Count | 532 | 625 | 389 | 293 | 311 | 547 | 696 | 581 | 783 | 760 | 535 | 728 | 239 | 367 | 439 | 348 | 184 | 192 | 461 | 523 | 301 | 256 | 307 | 313 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.2 | 9.6 | 9.9 | 8.6 | 6.1 | 6.8 | 6.7 | 6.8 | 5.6 | 6.0 | 5.7 | 5.7 | 9.8 | 9.5 | 9.2 | 10.7 | 13.6 | 14.3 | 9.6 | 10.2 | 11.0 |
Std Deviation (1e-4) | 2.7 | 2.3 | 2.6 | 2.0 | 1.6 | 1.6 | 1.7 | 1.9 | 1.9 | 1.6 | 1.4 | 1.6 | 2.8 | 3.1 | 3.0 | 3.5 | 4.3 | 6.3 | 2.4 | 2.5 | 2.9 |
Intensity Change (Log2) | – | – | – | -0.21 | -0.7 | -0.54 | -0.56 | -0.54 | -0.83 | -0.72 | -0.8 | -0.81 | -0.02 | -0.07 | -0.11 | 0.1 | 0.45 | 0.52 | -0.05 | 0.04 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3.3 | 2.5 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | -1.4 | -0.8 | 0.2 | 1.3 | -1.5 | -1.6 | -2.0 | -2.1 | 1.5 | 3.5 | 4.1 | 1.6 | 3.3 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.5 | -0.8 | 2.6 | 2.1 | 2.7 | -1.2 | 0.9 | 0 | -1.4 | 1.7 | 0.8 | 2.9 | -0.6 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 6.2 | 2.7 | 3.1 | 2.6 | 8.0 | 6.1 | 7.6 | 7.6 | 0 | 0 | 0 | 4.5 | 0 | 2.6 |
Nucleus | -1.8 | -1.8 | -2.4 | -3.0 | -0.9 | -2.0 | -1.0 | -0.5 | 0.2 | -0.8 | -2.9 | -3.0 | 2.6 | 0 | 0.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.6 | 0.8 | -0.8 | 0.2 | -2.4 | -1.4 | -2.1 | -0.4 | -2.9 | 1.3 | -0.1 | 0.2 | -1.5 | -1.5 | -1.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2.1 | 0.4 | 3.5 | 4.7 | 4.1 | 2.7 | 4.2 | 0.1 | 0.3 | 0.5 | 2.6 | 2.3 | -6.3 | -5.1 | -4.1 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.1621 | 11.9156 | 10.3506 | 9.693 | 10.3087 | 10.3349 | 10.3852 | 15.3347 | 13.0148 | 11.3454 | 13.018 | 13.0162 | 10.6863 | 11.0685 | 10.4345 | 9.3973 | 10.3193 | 10.4072 |
Actin | 0.0199 | 0 | 0.0074 | 0 | 0.0002 | 0.0021 | 0.0005 | 0 | 0.0162 | 0.0734 | 0.0002 | 0 | 0.0158 | 0.0003 | 0.0013 | 0.0085 | 0 | 0.001 |
Bud | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0008 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Bud Neck | 0.0057 | 0.0003 | 0.0004 | 0.0002 | 0.0014 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0.0004 | 0.0012 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0003 |
Bud Periphery | 0.0004 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0013 | 0.0004 | 0.0017 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 |
Bud Site | 0.0066 | 0.0002 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0005 | 0.0019 | 0.0006 | 0.0019 | 0 | 0.0014 | 0.0012 | 0.0002 | 0.0008 | 0 | 0.0002 |
Cell Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.001 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0 |
Cytoplasm | 0.001 | 0 | 0.0007 | 0 | 0 | 0 | 0.1292 | 0 | 0.0029 | 0.0002 | 0.0035 | 0 | 0.0048 | 0 | 0.0003 | 0.0016 | 0 | 0 |
Cytoplasmic Foci | 0.0059 | 0.0001 | 0.0074 | 0.0001 | 0.0013 | 0.0008 | 0.0319 | 0.0001 | 0.0236 | 0.0161 | 0.0053 | 0.0002 | 0.012 | 0.0025 | 0.0083 | 0.0175 | 0 | 0.0019 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0011 | 0 | 0.0009 | 0 | 0 | 0 | 0.0046 | 0 | 0.0064 | 0.0002 | 0.002 | 0 | 0.0019 | 0 | 0.0004 | 0.0082 | 0 | 0 |
Endosome | 0.0046 | 0 | 0.0067 | 0 | 0.0003 | 0.0008 | 0.0059 | 0 | 0.0185 | 0.012 | 0.1139 | 0 | 0.0157 | 0.0002 | 0.0034 | 0.0049 | 0 | 0.0004 |
Golgi | 0.0066 | 0 | 0.0021 | 0 | 0.0003 | 0.002 | 0.001 | 0 | 0.0148 | 0.0531 | 0.006 | 0 | 0.0045 | 0.0003 | 0.0009 | 0.0033 | 0 | 0.001 |
Lipid Particles | 0.0095 | 0.0003 | 0.0048 | 0.0002 | 0.0048 | 0.0023 | 0.0195 | 0.0002 | 0.0146 | 0.0128 | 0.0033 | 0.0013 | 0.0045 | 0.0018 | 0.0114 | 0.0199 | 0.0001 | 0.0027 |
Mitochondria | 0.0151 | 0 | 0.0037 | 0.0001 | 0.0037 | 0.0013 | 0.008 | 0.0011 | 0.017 | 0.0065 | 0.0036 | 0.0001 | 0.0012 | 0.0009 | 0.0038 | 0.0158 | 0 | 0.0004 |
None | 0.0017 | 0 | 0.0026 | 0 | 0.0001 | 0 | 0.0034 | 0 | 0.0041 | 0.0002 | 0.0004 | 0 | 0.0007 | 0 | 0.0001 | 0.0029 | 0 | 0 |
Nuclear Periphery | 0.007 | 0 | 0.0049 | 0 | 0.0013 | 0.0001 | 0.0054 | 0 | 0.0239 | 0.0001 | 0.0033 | 0 | 0.0061 | 0 | 0.001 | 0.0108 | 0 | 0.0001 |
Nucleolus | 0.8159 | 0.867 | 0.867 | 0.9189 | 0.8454 | 0.8952 | 0.6886 | 0.8253 | 0.748 | 0.7062 | 0.7358 | 0.9053 | 0.8296 | 0.84 | 0.8715 | 0.7238 | 0.8851 | 0.8919 |
Nucleus | 0.0872 | 0.1291 | 0.0833 | 0.0793 | 0.0891 | 0.0829 | 0.0544 | 0.1711 | 0.0727 | 0.0706 | 0.0755 | 0.0875 | 0.0837 | 0.148 | 0.0839 | 0.15 | 0.1095 | 0.0941 |
Peroxisomes | 0.0049 | 0 | 0.0026 | 0 | 0.0004 | 0.002 | 0.0016 | 0 | 0.02 | 0.0457 | 0.0005 | 0 | 0.0017 | 0.0019 | 0.0047 | 0.0114 | 0 | 0.001 |
Punctate Nuclear | 0.0048 | 0.0023 | 0.002 | 0.0005 | 0.0502 | 0.0099 | 0.0311 | 0.0008 | 0.0021 | 0.0008 | 0.0102 | 0.0052 | 0.013 | 0.0019 | 0.007 | 0.013 | 0.0046 | 0.0044 |
Vacuole | 0.0007 | 0.0004 | 0.0011 | 0.0003 | 0.0004 | 0.0001 | 0.0072 | 0.0002 | 0.0051 | 0.0002 | 0.0243 | 0.0001 | 0.0013 | 0.0003 | 0.0009 | 0.0022 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0005 | 0 | 0.0006 | 0.0001 | 0.0004 | 0 | 0.0051 | 0.0002 | 0.0043 | 0.0002 | 0.0062 | 0 | 0.0006 | 0.0001 | 0.0005 | 0.0038 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 32.862 | 31.6363 | 25.9908 | 28.8423 | 27.0391 | 32.8438 | 29.4171 | 19.2762 | 25.3563 | 34.4046 |
Translational Efficiency | 3.4997 | 2.6678 | 2.9045 | 2.2232 | 3.3553 | 2.2284 | 1.7574 | 2.0858 | 1.6693 | 1.8923 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
701 | 343 | 164 | 1601 | 1526 | 1598 | 1847 | 1381 | 2227 | 1941 | 2011 | 2982 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 872.79 | 1265.98 | 1256.11 | 1262.09 | 898.70 | 1211.11 | 1174.42 | 1161.89 | 890.54 | 1220.81 | 1181.08 | 1215.69 |
Standard Deviation | 162.25 | 216.45 | 202.98 | 282.08 | 171.00 | 204.20 | 233.40 | 246.28 | 168.73 | 207.47 | 232.15 | 270.75 |
Intensity Change Log 2 | — | 0.536548 | 0.525256 | 0.532108 | — | 0.430418 | 0.386037 | 0.370562 | — | 0.483683 | 0.456308 | 0.452414 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000280 | 0.000274 | 0.000486 | 0.000671 | 0.000225 | 0.000221 | 0.000464 | 0.000566 | 0.000242 | 0.000230 | 0.000466 | 0.000622 |
Bud Neck | 0.001337 | 0.002707 | 0.005921 | 0.014459 | 0.001458 | 0.002509 | 0.006614 | 0.015044 | 0.001420 | 0.002544 | 0.006557 | 0.014730 |
Bud Site | 0.001501 | 0.000479 | 0.001049 | 0.002493 | 0.000291 | 0.000429 | 0.000847 | 0.004564 | 0.000671 | 0.000438 | 0.000863 | 0.003452 |
Cell Periphery | 0.000076 | 0.000023 | 0.000034 | 0.000151 | 0.000028 | 0.000159 | 0.000066 | 0.000221 | 0.000043 | 0.000135 | 0.000064 | 0.000183 |
Cytoplasm | 0.000628 | 0.000063 | 0.000236 | 0.001332 | 0.000264 | 0.000356 | 0.000409 | 0.004092 | 0.000379 | 0.000304 | 0.000395 | 0.002610 |
Cytoplasmic Foci | 0.000397 | 0.001468 | 0.000881 | 0.002061 | 0.000508 | 0.000402 | 0.000451 | 0.003095 | 0.000473 | 0.000590 | 0.000486 | 0.002540 |
Eisosomes | 0.000079 | 0.000111 | 0.000082 | 0.000131 | 0.000107 | 0.000139 | 0.000096 | 0.000095 | 0.000098 | 0.000134 | 0.000095 | 0.000114 |
Endoplasmic Reticulum | 0.001283 | 0.000433 | 0.000822 | 0.000662 | 0.000850 | 0.000584 | 0.000550 | 0.000871 | 0.000986 | 0.000557 | 0.000572 | 0.000759 |
Endosome | 0.000226 | 0.000834 | 0.000391 | 0.001874 | 0.000229 | 0.000670 | 0.000182 | 0.002592 | 0.000229 | 0.000699 | 0.000199 | 0.002206 |
Golgi | 0.000369 | 0.000252 | 0.000384 | 0.001580 | 0.000312 | 0.000531 | 0.000206 | 0.001606 | 0.000330 | 0.000481 | 0.000221 | 0.001592 |
Lipid Particles | 0.000137 | 0.000597 | 0.000081 | 0.000207 | 0.000196 | 0.000122 | 0.000032 | 0.000290 | 0.000177 | 0.000206 | 0.000036 | 0.000245 |
Mitochondria | 0.001396 | 0.001679 | 0.001244 | 0.004090 | 0.000621 | 0.002023 | 0.001544 | 0.003169 | 0.000865 | 0.001962 | 0.001520 | 0.003664 |
Mitotic Spindle | 0.003507 | 0.004617 | 0.009636 | 0.012818 | 0.005140 | 0.004036 | 0.004520 | 0.010624 | 0.004626 | 0.004139 | 0.004937 | 0.011802 |
None | 0.002966 | 0.000328 | 0.000499 | 0.001816 | 0.000945 | 0.000880 | 0.001232 | 0.003478 | 0.001582 | 0.000783 | 0.001173 | 0.002586 |
Nuclear Periphery | 0.001242 | 0.000948 | 0.000442 | 0.001049 | 0.000628 | 0.000342 | 0.000347 | 0.000939 | 0.000821 | 0.000449 | 0.000355 | 0.000998 |
Nuclear Periphery Foci | 0.003187 | 0.001775 | 0.001490 | 0.001275 | 0.002698 | 0.000864 | 0.000333 | 0.001044 | 0.002852 | 0.001025 | 0.000427 | 0.001168 |
Nucleolus | 0.589064* | 0.652961* | 0.696823* | 0.559336* | 0.639684* | 0.593210* | 0.564965* | 0.540312* | 0.623750* | 0.603768* | 0.575718* | 0.550526* |
Nucleus | 0.384404* | 0.311927* | 0.253543* | 0.378369* | 0.332911* | 0.382306* | 0.406893* | 0.393213* | 0.349119* | 0.369869* | 0.394387* | 0.385244* |
Peroxisomes | 0.007105 | 0.017071 | 0.024025 | 0.006608 | 0.012260 | 0.007191 | 0.008954 | 0.007170 | 0.010638 | 0.008937 | 0.010183 | 0.006869 |
Vacuole | 0.000450 | 0.000872 | 0.001203 | 0.004358 | 0.000344 | 0.001600 | 0.000582 | 0.004275 | 0.000377 | 0.001471 | 0.000633 | 0.004320 |
Vacuole Periphery | 0.000366 | 0.000581 | 0.000726 | 0.004662 | 0.000299 | 0.001427 | 0.000713 | 0.002740 | 0.000320 | 0.001277 | 0.000714 | 0.003772 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.08 | -3.59 | -9.78 | -6.94 | 0.07 | -0.02 | -11.31 | -12.12 | -11.18 | -4.10 | 0.37 | -11.72 | -15.27 | -13.85 | -5.44 |
Bud Neck | -1.66 | -4.81 | -13.34 | -12.20 | -5.78 | -4.50 | -14.72 | -14.58 | -13.38 | -9.20 | -4.21 | -14.56 | -20.24 | -18.59 | -12.19 |
Bud Site | 1.10 | -0.02 | -1.14 | -6.22 | -2.57 | -1.49 | -6.89 | -6.34 | -6.10 | -5.44 | 0.79 | -0.73 | -6.05 | -8.69 | -7.34 |
Cell Periphery | 2.00 | 1.24 | -2.63 | -6.35 | -5.26 | -1.40 | -4.28 | -6.19 | -0.62 | -4.77 | -1.19 | -2.11 | -7.45 | -0.62 | -6.27 |
Cytoplasm | 1.94 | 1.05 | -1.82 | -3.51 | -2.84 | -0.41 | -1.33 | -5.12 | -4.97 | -4.82 | 0.36 | -0.70 | -5.56 | -5.64 | -5.28 |
Cytoplasmic Foci | -1.45 | -1.09 | -4.17 | -0.72 | -2.31 | 0.59 | 0.18 | -3.39 | -3.58 | -3.48 | -0.59 | -0.17 | -4.93 | -4.57 | -4.99 |
Eisosomes | -3.57 | -1.11 | -4.91 | -1.32 | -4.01 | -3.81 | 1.55 | 2.09 | 6.72 | 0.74 | -5.38 | 0.50 | -1.95 | 3.36 | -2.64 |
Endoplasmic Reticulum | 4.52 | -0.02 | 2.72 | -2.70 | 1.80 | 2.79 | 2.92 | -0.73 | -2.82 | -2.88 | 4.79 | 3.80 | 1.32 | -3.27 | -2.27 |
Endosome | -0.91 | -0.97 | -3.91 | -1.30 | -2.80 | -2.07 | 0.48 | -3.98 | -3.01 | -4.08 | -2.26 | 0.28 | -5.54 | -3.59 | -5.62 |
Golgi | 0.67 | -0.78 | -2.41 | -2.77 | -2.23 | -0.98 | 2.57 | -2.31 | -1.76 | -2.50 | -0.79 | 1.63 | -3.43 | -2.69 | -3.74 |
Lipid Particles | -1.33 | -0.35 | -1.16 | 1.12 | -0.70 | 0.54 | 2.26 | -0.93 | -1.49 | -3.27 | -0.48 | 2.61 | -1.08 | -0.30 | -4.60 |
Mitochondria | -0.30 | 0.05 | -3.28 | -2.71 | -4.92 | -2.09 | -7.82 | -5.30 | -1.44 | -3.26 | -1.83 | -3.04 | -6.74 | -2.64 | -5.63 |
Mitotic Spindle | -0.37 | -2.10 | -3.92 | -3.47 | -0.12 | 0.79 | 0.15 | -3.48 | -4.09 | -3.70 | 0.44 | -0.76 | -5.28 | -5.72 | -4.71 |
None | 2.87 | 2.58 | 1.08 | -3.23 | -2.64 | 0.11 | -0.95 | -3.33 | -3.34 | -3.15 | 1.74 | 1.03 | -1.91 | -3.68 | -3.33 |
Nuclear Periphery | 0.25 | 0.74 | 0.12 | -0.28 | -3.17 | 0.73 | 0.69 | -0.84 | -2.79 | -2.81 | 0.87 | 1.10 | -0.58 | -3.44 | -5.18 |
Nuclear Periphery Foci | 1.73 | 1.46 | 2.71 | 0.68 | 0.61 | 3.62 | 5.00 | 3.26 | -0.67 | -3.25 | 4.37 | 6.23 | 3.94 | -0.60 | -3.21 |
Nucleolus | -4.17 | -3.73 | 3.43 | 7.19 | 5.71 | 5.71 | 8.92 | 11.71 | 6.93 | 3.74 | 2.75 | 6.57 | 11.84 | 8.66 | 4.10 |
Nucleus | 4.72 | 5.39 | 0.04 | -5.00 | -5.53 | -6.11 | -8.83 | -7.43 | -2.22 | 0.45 | -2.89 | -6.09 | -6.16 | -3.07 | 0.49 |
Peroxisomes | -4.94 | -7.58 | 0.75 | 5.26 | 7.92 | 7.19 | 4.53 | 7.16 | 0.32 | 4.24 | 2.64 | 0.74 | 7.19 | 4.39 | 8.01 |
Vacuole | -1.24 | -2.29 | -5.15 | -4.42 | -2.28 | -3.21 | -2.59 | -5.11 | -3.43 | -4.65 | -3.32 | -3.13 | -7.30 | -4.93 | -6.40 |
Vacuole Periphery | -1.83 | -2.83 | -6.98 | -6.54 | -6.11 | -3.57 | -8.61 | -7.54 | -2.79 | -6.12 | -3.66 | -8.36 | -9.53 | -5.46 | -8.30 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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