Standard name | |
---|---|
Human Ortholog | |
Description | GPI lipid remodelase; responsible for introducing ceramides into GPI anchors having a C26:0 fatty acid in sn-2 of the glycerol moiety; can also use lyso-GPI protein anchors and various base resistant lipids as substrates; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 11 | 17 | 43 | 31 | 21 | 12 | 24 | 40 | – | 42 | 30 | 33 | 3 | 8 | 7 | 18 | 41 | 67 | 8 | 7 | 38 | 11 | 19 | 16 |
Bud | 0 | 2 | 4 | 10 | 4 | 16 | 17 | 23 | – | 32 | 47 | 43 | 0 | 1 | 0 | 1 | 8 | 7 | 2 | 1 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 3 | 16 | 10 | 14 | 13 | 32 | 26 | – | 34 | 28 | 85 | 10 | 15 | 21 | 13 | 16 | 38 | 1 | 1 | 11 | 6 | 12 | 17 |
Cytoplasm | 44 | 39 | 46 | 55 | 52 | 71 | 124 | 113 | – | 104 | 149 | 155 | 22 | 11 | 28 | 138 | 167 | 68 | 4 | 9 | 3 | 2 | 3 | 13 |
Endoplasmic Reticulum | 140 | 108 | 311 | 133 | 158 | 200 | 318 | 296 | – | 299 | 98 | 160 | 122 | 108 | 140 | 10 | 14 | 9 | 257 | 160 | 398 | 120 | 151 | 234 |
Endosome | 7 | 1 | 4 | 9 | 17 | 10 | 14 | 8 | – | 10 | 0 | 3 | 0 | 0 | 1 | 5 | 19 | 7 | 1 | 3 | 4 | 0 | 3 | 3 |
Golgi | 1 | 0 | 1 | 4 | 3 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 14 | 3 | 2 | 6 | 2 | 6 | 10 |
Mitochondria | 13 | 26 | 107 | 164 | 151 | 306 | 442 | 431 | – | 626 | 453 | 607 | 1 | 2 | 0 | 41 | 52 | 33 | 11 | 10 | 22 | 16 | 20 | 16 |
Nucleus | 0 | 1 | 0 | 3 | 0 | 1 | 3 | 4 | – | 4 | 2 | 0 | 1 | 0 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 2 | 5 | 3 | 7 | 2 | 5 | 11 | 15 | – | 21 | 3 | 10 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 2 | 3 | 1 | 2 | 2 | – | 3 | 0 | 3 | 0 | 0 | 0 | 1 | 16 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | – | 3 | 1 | 1 | 0 | 0 | 0 | 4 | 4 | 3 | 2 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 34 | 4 | 19 | 13 | 64 | 50 | 116 | 89 | – | 53 | 46 | 70 | 7 | 3 | 5 | 12 | 45 | 17 | 4 | 12 | 3 | 5 | 5 | 6 |
Unique Cell Count | 228 | 169 | 430 | 325 | 381 | 513 | 827 | 813 | 907 | 654 | 883 | 151 | 128 | 180 | 215 | 329 | 223 | 303 | 215 | 499 | 172 | 230 | 327 | |
Labelled Cell Count | 256 | 206 | 555 | 441 | 489 | 687 | 1104 | 1050 | 1234 | 861 | 1170 | 167 | 148 | 205 | 245 | 390 | 272 | 303 | 215 | 499 | 172 | 230 | 327 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 7.0 | 5.9 | 6.4 | 6.7 | 5.8 | 5.8 | 5.9 | – | 5.0 | 5.1 | 5.3 | 9.1 | 9.0 | 9.5 | 5.5 | 6.5 | 8.4 | 8.8 | 9.6 | 10.2 |
Std Deviation (1e-4) | 1.2 | 1.0 | 0.9 | 1.2 | 1.4 | 1.1 | 1.2 | 1.1 | – | 0.9 | 1.1 | 0.9 | 1.1 | 1.0 | 1.1 | 1.5 | 1.3 | 1.8 | 1.6 | 2.0 | 2.4 |
Intensity Change (Log2) | – | – | – | 0.13 | 0.17 | -0.03 | -0.02 | -0.01 | – | -0.24 | -0.21 | -0.16 | 0.63 | 0.61 | 0.68 | -0.1 | 0.13 | 0.51 | 0.58 | 0.71 | 0.8 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.2 | -2.4 | -5.0 | -5.3 | -3.4 | – | -3.8 | -3.5 | -4.6 | -3.1 | -1.3 | -2.5 | -0.7 | 1.1 | 6.5 |
Bud | 2.2 | 0 | 2.3 | 1.5 | 2.2 | – | 2.7 | 4.8 | 3.6 | 0 | 0 | 0 | 0 | 1.6 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.5 | 0 | -1.1 | 0.1 | -0.5 | – | 0 | 0.5 | 3.8 | 1.5 | 3.5 | 3.7 | 1.3 | 0.8 | 5.9 |
Cytoplasm | 2.5 | 1.3 | 1.5 | 2.1 | 1.6 | – | 0.4 | 5.1 | 3.2 | 1.3 | -0.7 | 1.7 | 14.2 | 12.2 | 6.3 |
Endoplasmic Reticulum | -8.7 | -8.9 | -10.2 | -11.4 | -12.1 | – | -13.5 | -19.1 | -19.2 | 2.1 | 2.8 | 1.4 | -16.2 | -18.8 | -16.6 |
Endosome | 1.9 | 3.2 | 1.3 | 1.1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.9 |
Mitochondria | 7.3 | 4.5 | 10.7 | 9.7 | 9.5 | – | 15.1 | 14.3 | 15.0 | -6.6 | -5.8 | -7.4 | -1.7 | -3.0 | -3.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1.6 | – | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.6 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.3 | 5.8 | 3.1 | 5.2 | 3.9 | – | 1.1 | 1.8 | 2.4 | 0.1 | -1.1 | -1.0 | 0.7 | 4.5 | 1.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.1104 | 6.2804 | 5.9646 | 5.2768 | 5.5901 | 5.847 | 4.2446 | 6.2781 | 5.2654 | 4.5669 | 4.7332 | 5.0725 | 4.4539 | 4.8691 | 4.7942 | 4.6569 | 5.0744 | 4.7164 |
Actin | 0.0065 | 0.0167 | 0.0055 | 0.0013 | 0.0036 | 0.0086 | 0.0691 | 0.0003 | 0.021 | 0.0122 | 0.0034 | 0.0145 | 0.039 | 0 | 0.0323 | 0.0499 | 0.0038 | 0.0194 |
Bud | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0002 | 0 | 0.0002 |
Bud Neck | 0.0009 | 0.0005 | 0.0015 | 0.0006 | 0.0014 | 0.0019 | 0.0013 | 0.0001 | 0.0024 | 0.0007 | 0.0006 | 0.0009 | 0.0006 | 0 | 0.0013 | 0.0015 | 0.0013 | 0.0036 |
Bud Periphery | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0009 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0007 | 0.0005 | 0.0001 | 0.0006 |
Bud Site | 0.0007 | 0.0044 | 0.0004 | 0.0003 | 0.0009 | 0.0003 | 0.0057 | 0.0004 | 0.0024 | 0.0001 | 0.0032 | 0.0001 | 0.0005 | 0 | 0.0016 | 0.0005 | 0 | 0.0002 |
Cell Periphery | 0.0022 | 0.0037 | 0.0043 | 0.0027 | 0.0031 | 0.0026 | 0.0027 | 0.0049 | 0.0103 | 0.0024 | 0.0041 | 0.0012 | 0.0022 | 0.0005 | 0.0037 | 0.0032 | 0.0038 | 0.0026 |
Cytoplasm | 0.038 | 0.0718 | 0.0422 | 0.0349 | 0.0779 | 0.0441 | 0.0227 | 0.0248 | 0.0351 | 0.0186 | 0.0164 | 0.0324 | 0.0161 | 0.0041 | 0.0767 | 0.0419 | 0.0139 | 0.0426 |
Cytoplasmic Foci | 0.0061 | 0.008 | 0.0054 | 0.0053 | 0.0089 | 0.0082 | 0.0299 | 0.0029 | 0.0086 | 0.0066 | 0.0048 | 0.0071 | 0.0028 | 0.0001 | 0.0036 | 0.0047 | 0.0028 | 0.0092 |
Eisosomes | 0.0006 | 0.0005 | 0.0003 | 0.0002 | 0.0006 | 0.0002 | 0.0008 | 0.0001 | 0.0006 | 0.0007 | 0.0006 | 0.0002 | 0.0006 | 0 | 0.0004 | 0.0003 | 0.0002 | 0.0006 |
Endoplasmic Reticulum | 0.8607 | 0.7869 | 0.8771 | 0.8938 | 0.8311 | 0.8456 | 0.7134 | 0.9117 | 0.8373 | 0.8989 | 0.8662 | 0.8544 | 0.8664 | 0.9823 | 0.776 | 0.8653 | 0.7768 | 0.8297 |
Endosome | 0.0284 | 0.0294 | 0.0166 | 0.0191 | 0.0226 | 0.0255 | 0.0306 | 0.0137 | 0.0185 | 0.0174 | 0.0195 | 0.0226 | 0.0164 | 0.0013 | 0.0193 | 0.0074 | 0.0061 | 0.0151 |
Golgi | 0.0122 | 0.0165 | 0.0107 | 0.0168 | 0.0108 | 0.0157 | 0.0196 | 0.0061 | 0.0208 | 0.0141 | 0.0207 | 0.0216 | 0.0056 | 0.0001 | 0.0193 | 0.0116 | 0.0178 | 0.0155 |
Lipid Particles | 0.0104 | 0.0325 | 0.0112 | 0.0102 | 0.0201 | 0.0118 | 0.0258 | 0.0225 | 0.0188 | 0.014 | 0.0324 | 0.0158 | 0.0041 | 0.0008 | 0.005 | 0.0018 | 0.1688 | 0.0397 |
Mitochondria | 0.0047 | 0.0085 | 0.0019 | 0.0016 | 0.0015 | 0.0063 | 0.0068 | 0.0006 | 0.0019 | 0.0021 | 0.0007 | 0.0018 | 0.0012 | 0 | 0.0095 | 0.0027 | 0.0001 | 0.0014 |
None | 0.0024 | 0.0019 | 0.0081 | 0.0005 | 0.0011 | 0.0045 | 0.008 | 0.0006 | 0.0021 | 0.0012 | 0.0096 | 0.0084 | 0.0057 | 0.0002 | 0.032 | 0.0003 | 0.0002 | 0.0041 |
Nuclear Periphery | 0.0166 | 0.0082 | 0.0083 | 0.0078 | 0.0082 | 0.0186 | 0.0181 | 0.0058 | 0.0087 | 0.0061 | 0.0087 | 0.0123 | 0.0301 | 0.008 | 0.0117 | 0.005 | 0.0025 | 0.0101 |
Nucleolus | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Nucleus | 0.0017 | 0.0013 | 0.0006 | 0.0005 | 0.0015 | 0.0011 | 0.0026 | 0.0003 | 0.0007 | 0.0004 | 0.0012 | 0.0004 | 0.0021 | 0.0003 | 0.001 | 0.0003 | 0.0002 | 0.0008 |
Peroxisomes | 0.0007 | 0.0002 | 0.0012 | 0.0001 | 0.0009 | 0.0004 | 0.0225 | 0 | 0.0033 | 0.0003 | 0.0001 | 0.0018 | 0.001 | 0 | 0.0003 | 0.0001 | 0 | 0.0002 |
Punctate Nuclear | 0.0011 | 0.0006 | 0.0002 | 0.0002 | 0.0019 | 0.0005 | 0.0121 | 0.0002 | 0.0005 | 0.0003 | 0.0009 | 0.0003 | 0.002 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.001 |
Vacuole | 0.0046 | 0.0063 | 0.0035 | 0.0033 | 0.0027 | 0.0028 | 0.0046 | 0.0045 | 0.0052 | 0.0028 | 0.0053 | 0.0028 | 0.0019 | 0.0021 | 0.0033 | 0.0018 | 0.0014 | 0.0028 |
Vacuole Periphery | 0.001 | 0.001 | 0.0007 | 0.0007 | 0.0006 | 0.0009 | 0.0017 | 0.0006 | 0.0009 | 0.0008 | 0.001 | 0.0011 | 0.0012 | 0.0002 | 0.0016 | 0.0009 | 0.0002 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 72.0953 | 67.8952 | 57.0305 | 73.9674 | 46.2375 | 60.881 | 78.8416 | 90.1971 | 77.5904 | 82.89 |
Translational Efficiency | 0.5238 | 0.4802 | 0.623 | 0.4234 | 0.5448 | 0.7124 | 0.5593 | 0.3611 | 0.4565 | 0.4225 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
91 | 1025 | 221 | 1069 | 2220 | 578 | 1943 | 889 | 2311 | 1603 | 2164 | 1958 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1076.84 | 854.42 | 1257.36 | 1038.63 | 881.74 | 920.00 | 1021.12 | 1049.85 | 889.42 | 878.07 | 1045.25 | 1043.72 |
Standard Deviation | 126.67 | 147.48 | 188.05 | 151.91 | 134.15 | 125.45 | 134.99 | 148.49 | 139.14 | 143.43 | 158.40 | 150.47 |
Intensity Change Log 2 | — | -0.333787 | 0.223594 | -0.052122 | — | 0.061281 | 0.211727 | 0.251758 | — | -0.142461 | 0.218264 | 0.092645 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003235 | 0.005191 | 0.000413 | 0.012139 | 0.007512 | 0.001966 | 0.001695 | 0.006216 | 0.007344 | 0.004028 | 0.001564 | 0.009450 |
Bud Neck | 0.003448 | 0.001035 | 0.002585 | 0.004104 | 0.000604 | 0.000870 | 0.000169 | 0.001209 | 0.000716 | 0.000976 | 0.000416 | 0.002789 |
Bud Site | 0.011013 | 0.008249 | 0.004212 | 0.027194 | 0.006288 | 0.001768 | 0.000879 | 0.006056 | 0.006474 | 0.005912 | 0.001219 | 0.017596 |
Cell Periphery | 0.006210 | 0.003091 | 0.000756 | 0.003573 | 0.021527 | 0.003973 | 0.001188 | 0.001512 | 0.020924 | 0.003409 | 0.001144 | 0.002637 |
Cytoplasm | 0.053113 | 0.060169 | 0.014466 | 0.056156 | 0.030472 | 0.080414 | 0.017043 | 0.031360 | 0.031364 | 0.067469 | 0.016780 | 0.044898 |
Cytoplasmic Foci | 0.038533 | 0.057930 | 0.002276 | 0.014807 | 0.016261 | 0.024794 | 0.001828 | 0.013457 | 0.017138 | 0.045982 | 0.001874 | 0.014194 |
Eisosomes | 0.000818 | 0.001196 | 0.000020 | 0.000900 | 0.004088 | 0.001912 | 0.000234 | 0.000557 | 0.003959 | 0.001454 | 0.000212 | 0.000744 |
Endoplasmic Reticulum | 0.441555* | 0.650248* | 0.789834* | 0.727898* | 0.762134* | 0.696579* | 0.942119* | 0.785642* | 0.749511* | 0.666954* | 0.926567* | 0.754116* |
Endosome | 0.050309 | 0.024393 | 0.021906 | 0.016075 | 0.005156 | 0.009636 | 0.006956 | 0.019981 | 0.006933 | 0.019072 | 0.008483 | 0.017848 |
Golgi | 0.017660 | 0.064988 | 0.002796 | 0.008085 | 0.017962 | 0.047865 | 0.005312 | 0.010239 | 0.017950 | 0.058814 | 0.005055 | 0.009063 |
Lipid Particles | 0.068237 | 0.074048 | 0.006033 | 0.044145 | 0.032359 | 0.082012 | 0.009591 | 0.039607 | 0.033772 | 0.076920 | 0.009228 | 0.042084 |
Mitochondria | 0.000871 | 0.009775 | 0.000209 | 0.002187 | 0.009704 | 0.009622 | 0.000808 | 0.000661 | 0.009356 | 0.009720 | 0.000747 | 0.001494 |
Mitotic Spindle | 0.022428 | 0.000452 | 0.001015 | 0.002442 | 0.000162 | 0.000526 | 0.000021 | 0.003642 | 0.001039 | 0.000479 | 0.000122 | 0.002987 |
None | 0.000228 | 0.003241 | 0.000113 | 0.001031 | 0.007071 | 0.004862 | 0.005016 | 0.001651 | 0.006802 | 0.003826 | 0.004516 | 0.001313 |
Nuclear Periphery | 0.027321 | 0.001256 | 0.024546 | 0.003867 | 0.000120 | 0.002389 | 0.000326 | 0.008026 | 0.001191 | 0.001665 | 0.002800 | 0.005756 |
Nuclear Periphery Foci | 0.095190 | 0.010989 | 0.111360 | 0.035021 | 0.000461 | 0.008498 | 0.003141 | 0.054064 | 0.004191 | 0.010091 | 0.014193 | 0.043667 |
Nucleolus | 0.000250 | 0.000435 | 0.000013 | 0.000098 | 0.000016 | 0.000179 | 0.000006 | 0.000063 | 0.000025 | 0.000343 | 0.000007 | 0.000082 |
Nucleus | 0.006976 | 0.001653 | 0.003165 | 0.008778 | 0.000538 | 0.004190 | 0.000321 | 0.003224 | 0.000792 | 0.002568 | 0.000612 | 0.006256 |
Peroxisomes | 0.001802 | 0.009859 | 0.000036 | 0.001203 | 0.076790 | 0.012331 | 0.001895 | 0.002223 | 0.073837 | 0.010750 | 0.001705 | 0.001666 |
Vacuole | 0.145445 | 0.007847 | 0.012806 | 0.028914 | 0.000263 | 0.004192 | 0.001227 | 0.010062 | 0.005980 | 0.006529 | 0.002410 | 0.020355 |
Vacuole Periphery | 0.005357 | 0.003954 | 0.001439 | 0.001382 | 0.000512 | 0.001421 | 0.000222 | 0.000548 | 0.000702 | 0.003041 | 0.000346 | 0.001003 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.18 | 1.85 | -3.76 | -3.50 | -6.24 | 5.47 | 6.00 | 0.75 | -2.73 | -2.95 | 3.43 | 6.28 | -1.50 | -4.14 | -6.13 |
Bud Neck | 4.11 | 1.00 | -0.79 | -6.44 | -1.82 | -1.11 | 3.55 | -2.88 | -1.47 | -4.83 | -1.52 | 2.05 | -7.31 | -6.37 | -8.28 |
Bud Site | 0.88 | 1.75 | -3.26 | -5.81 | -6.62 | 5.11 | 6.45 | 0.00 | -3.67 | -4.51 | 0.55 | 6.32 | -5.78 | -6.16 | -8.98 |
Cell Periphery | 0.65 | 1.16 | 0.54 | -0.50 | -3.64 | 10.91 | 14.93 | 14.13 | 2.54 | -0.75 | 12.42 | 14.96 | 13.23 | 1.16 | -3.29 |
Cytoplasm | -0.37 | 3.41 | -0.14 | 0.58 | -7.56 | -9.33 | 7.92 | -0.66 | 7.96 | -4.85 | -12.06 | 8.56 | -5.53 | 6.00 | -11.52 |
Cytoplasmic Foci | -2.27 | 5.41 | 3.50 | 10.96 | -8.07 | -2.98 | 12.82 | 1.00 | 3.29 | -6.41 | -10.49 | 13.72 | 1.54 | 11.22 | -10.61 |
Eisosomes | -0.94 | 2.19 | -0.27 | 1.18 | -7.38 | 5.13 | 12.97 | 10.41 | 3.89 | -1.85 | 7.50 | 13.11 | 10.68 | 3.48 | -4.98 |
Endoplasmic Reticulum | -5.43 | -8.34 | -7.08 | -4.49 | 3.56 | 4.32 | -26.36 | -1.43 | -4.81 | 15.46 | 7.80 | -24.97 | 0.50 | -6.99 | 23.07 |
Endosome | 3.00 | 3.21 | 3.94 | 4.48 | 2.35 | -3.87 | -3.46 | -9.09 | -5.28 | -7.73 | -11.55 | -2.45 | -10.46 | 0.68 | -8.77 |
Golgi | -6.26 | 2.09 | 1.35 | 15.54 | -3.79 | -7.48 | 10.33 | 4.57 | 9.27 | -3.43 | -14.18 | 10.77 | 6.79 | 17.92 | -4.23 |
Lipid Particles | -0.45 | 5.42 | 2.02 | 5.38 | -9.87 | -7.67 | 12.86 | -2.05 | 5.80 | -8.40 | -11.18 | 14.03 | -3.19 | 7.95 | -13.11 |
Mitochondria | -7.38 | 2.09 | -1.38 | 5.35 | -2.32 | 0.04 | 11.36 | 11.54 | 4.09 | 0.96 | -0.28 | 11.44 | 8.98 | 7.02 | -1.57 |
Mitotic Spindle | 1.91 | 1.86 | 1.62 | -2.44 | -1.89 | -1.21 | 8.39 | -2.71 | -2.39 | -2.81 | 1.11 | 1.97 | -2.62 | -3.43 | -3.87 |
None | -3.26 | 0.84 | -0.71 | 3.15 | -2.68 | 1.23 | 1.55 | 4.35 | 1.87 | 2.78 | 2.60 | 1.86 | 5.46 | 3.20 | 3.51 |
Nuclear Periphery | 3.24 | 0.23 | 2.90 | -2.98 | 5.09 | -4.33 | -3.16 | -7.02 | -4.56 | -6.83 | -1.19 | -3.00 | -6.12 | -5.59 | -3.67 |
Nuclear Periphery Foci | 4.85 | -0.90 | 3.34 | -8.04 | 6.17 | -4.72 | -7.28 | -13.25 | -10.45 | -12.55 | -4.45 | -6.19 | -16.07 | -13.03 | -10.83 |
Nucleolus | -0.04 | 1.48 | 1.17 | 1.55 | -4.22 | -1.30 | 3.56 | -2.92 | 0.85 | -3.42 | -2.14 | 2.19 | -3.08 | 1.76 | -5.65 |
Nucleus | 2.59 | 1.46 | -0.77 | -6.19 | -2.93 | -3.97 | 1.93 | -4.14 | 0.40 | -4.37 | -4.78 | 0.79 | -7.83 | -5.03 | -7.86 |
Peroxisomes | -8.00 | 4.84 | 1.80 | 9.51 | -7.89 | 18.16* | 23.88 | 23.59 | 5.77 | -0.68 | 20.15* | 23.83 | 23.85 | 10.47 | 0.04 |
Vacuole | 6.36 | 6.11 | 5.21 | -8.26 | -5.92 | -5.31 | -3.73 | -8.28 | -4.64 | -7.43 | -0.20 | 3.40 | -8.45 | -9.01 | -12.10 |
Vacuole Periphery | 0.47 | 1.39 | 1.42 | 4.28 | 0.16 | -3.72 | 4.77 | -0.33 | 3.21 | -2.51 | -7.55 | 2.77 | -1.14 | 5.45 | -2.64 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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