ORF | |
---|---|
Human Ortholog | |
Description | Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Bud | 0 | 0 | 1 | 3 | 0 | 3 | 6 | 14 | 15 | 7 | 9 | 14 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 7 | 6 | 2 | 6 | 10 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 27 | 15 | 46 | 168 | 183 | 318 | 378 | 486 | 336 | 364 | 399 | 415 | 120 | 94 | 106 | 86 | 48 | 26 | 2 | 2 | 5 | 2 | 3 | 4 |
Endoplasmic Reticulum | 3 | 1 | 0 | 1 | 1 | 1 | 2 | 10 | 3 | 3 | 1 | 4 | 19 | 7 | 14 | 10 | 9 | 5 | 0 | 0 | 2 | 1 | 1 | 1 |
Endosome | 18 | 9 | 87 | 69 | 70 | 31 | 20 | 38 | 23 | 10 | 11 | 30 | 87 | 66 | 55 | 39 | 12 | 10 | 27 | 22 | 32 | 27 | 47 | 49 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 5 | 0 | 2 | 2 | 1 | 1 |
Mitochondria | 14 | 7 | 26 | 55 | 36 | 191 | 308 | 314 | 193 | 301 | 295 | 326 | 2 | 1 | 0 | 14 | 19 | 4 | 3 | 2 | 7 | 6 | 7 | 14 |
Nucleus | 28 | 12 | 5 | 0 | 5 | 8 | 4 | 3 | 4 | 2 | 2 | 7 | 52 | 79 | 106 | 12 | 24 | 9 | 7 | 7 | 2 | 2 | 6 | 4 |
Nuclear Periphery | 1 | 8 | 4 | 0 | 1 | 0 | 4 | 2 | 1 | 0 | 0 | 0 | 2 | 1 | 3 | 5 | 5 | 1 | 7 | 11 | 26 | 8 | 16 | 10 |
Nucleolus | 5 | 3 | 7 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 0 | 3 | 3 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 3 | 4 | 5 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 267 | 154 | 287 | 141 | 183 | 144 | 125 | 146 | 74 | 89 | 87 | 144 | 551 | 590 | 636 | 179 | 138 | 93 | 278 | 149 | 305 | 186 | 305 | 275 |
Unique Cell Count | 318 | 183 | 360 | 341 | 368 | 515 | 645 | 797 | 490 | 575 | 615 | 689 | 724 | 738 | 808 | 292 | 204 | 125 | 339 | 206 | 392 | 242 | 397 | 378 |
Labelled Cell Count | 366 | 209 | 468 | 442 | 480 | 698 | 851 | 1024 | 656 | 778 | 810 | 955 | 840 | 848 | 926 | 351 | 255 | 148 | 339 | 206 | 392 | 242 | 397 | 378 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 6.7 | 5.8 | 5.4 | 5.4 | 4.5 | 4.2 | 4.5 | 4.8 | 4.1 | 4.5 | 4.5 | 6.6 | 7.1 | 7.3 | 5.7 | 6.2 | 7.0 | 7.0 | 7.1 | 7.3 |
Std Deviation (1e-4) | 1.0 | 1.2 | 1.4 | 1.3 | 1.5 | 1.4 | 1.1 | 1.1 | 2.2 | 0.8 | 1.2 | 0.9 | 0.9 | 0.9 | 0.9 | 1.2 | 1.3 | 1.5 | 1.8 | 1.6 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.12 | -0.39 | -0.46 | -0.37 | -0.28 | -0.49 | -0.38 | -0.37 | 0.19 | 0.28 | 0.33 | -0.02 | 0.09 | 0.27 | 0.28 | 0.29 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 10.5 | 10.7 | 14.5 | 14.1 | 15.2 | 16.2 | 15.2 | 15.8 | 14.7 | 1.6 | 0 | 0.2 | 5.3 | 3.3 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.3 | -1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.1 | -6.8 | -8.4 | -3.5 | -5.5 | -3.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.7 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.8 | -7.1 | -7.7 | -1.3 | 0.9 | -1.6 |
Nucleus | 0 | 0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 5.5 | 6.3 | 2.2 | 5.4 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -10.4 | -8.5 | -15.1 | -18.6 | -20.0 | -18.8 | -19.5 | -20.3 | -18.4 | -1.3 | 0.1 | -0.4 | -5.2 | -3.2 | -1.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.9221 | 2.196 | 2.3049 | 2.2586 | 2.6724 | 2.3056 | 3.2299 | 3.4947 | 3.087 | 3.6814 | 2.9873 | 3.086 | 3.9115 | 4.1633 | 3.9678 | 3.8879 | 3.7497 | 4.1703 |
Actin | 0.0019 | 0.0001 | 0.0019 | 0.0182 | 0.0032 | 0.0011 | 0.0061 | 0.0001 | 0.0001 | 0.0087 | 0 | 0.0163 | 0.0055 | 0.0001 | 0.0004 | 0.0003 | 0 | 0 |
Bud | 0.0004 | 0.0042 | 0.0015 | 0.0027 | 0.0021 | 0.0012 | 0.0008 | 0.0001 | 0.0016 | 0.0002 | 0.0005 | 0.0004 | 0.0003 | 0.0016 | 0.002 | 0.002 | 0.0006 | 0.0008 |
Bud Neck | 0.0011 | 0.0007 | 0.0008 | 0.0019 | 0.0003 | 0.0021 | 0.012 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0007 | 0.0005 | 0.0008 | 0.0025 | 0.0008 | 0.0003 | 0.0011 |
Bud Periphery | 0.0012 | 0.0033 | 0.0017 | 0.0036 | 0.0031 | 0.0015 | 0.0011 | 0.0002 | 0.0026 | 0.0003 | 0.0003 | 0.0006 | 0.0005 | 0.0011 | 0.0039 | 0.0028 | 0.0009 | 0.0015 |
Bud Site | 0.0014 | 0.0072 | 0.0024 | 0.0187 | 0.0108 | 0.0014 | 0.0104 | 0.0007 | 0.0024 | 0.0003 | 0.0025 | 0.0004 | 0.0008 | 0.0058 | 0.0068 | 0.0012 | 0.0006 | 0.0009 |
Cell Periphery | 0.003 | 0.0004 | 0.0003 | 0.0009 | 0.0007 | 0.0004 | 0.0012 | 0.0004 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0089 | 0.0201 | 0.0082 | 0.0194 | 0.0003 | 0.0144 |
Cytoplasm | 0.0219 | 0.1375 | 0.1261 | 0.0637 | 0.0691 | 0.0686 | 0.0767 | 0.0921 | 0.1335 | 0.0098 | 0.1633 | 0.1213 | 0.0695 | 0.0952 | 0.1029 | 0.1083 | 0.0449 | 0.0728 |
Cytoplasmic Foci | 0.0226 | 0.0262 | 0.042 | 0.0403 | 0.026 | 0.0312 | 0.0436 | 0.055 | 0.0272 | 0.03 | 0.0384 | 0.0226 | 0.0302 | 0.0192 | 0.0324 | 0.0362 | 0.0158 | 0.0241 |
Eisosomes | 0.0003 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0065 | 0.0066 | 0.0094 | 0.0016 | 0.004 | 0.005 | 0.0211 | 0.0089 | 0.0102 | 0.0016 | 0.0031 | 0.0083 | 0.0099 | 0.0077 | 0.0129 | 0.0023 | 0.0061 | 0.0058 |
Endosome | 0.3395 | 0.2494 | 0.3378 | 0.3444 | 0.2845 | 0.3237 | 0.268 | 0.3404 | 0.3872 | 0.3199 | 0.4952 | 0.2663 | 0.3782 | 0.2902 | 0.3631 | 0.3602 | 0.4001 | 0.3071 |
Golgi | 0.0078 | 0.0021 | 0.0063 | 0.0153 | 0.022 | 0.0062 | 0.0115 | 0.0024 | 0.0067 | 0.0043 | 0.0062 | 0.0055 | 0.0045 | 0.0029 | 0.0072 | 0.0117 | 0.0044 | 0.0036 |
Lipid Particles | 0.0089 | 0.0021 | 0.0077 | 0.0106 | 0.027 | 0.0052 | 0.0166 | 0.0076 | 0.0037 | 0.0068 | 0.0018 | 0.0049 | 0.0058 | 0.0045 | 0.0025 | 0.0008 | 0.0029 | 0.0033 |
Mitochondria | 0.0181 | 0.004 | 0.012 | 0.046 | 0.0586 | 0.0048 | 0.0085 | 0.0051 | 0.0097 | 0.003 | 0.0018 | 0.0048 | 0.0058 | 0.0027 | 0.0203 | 0.0056 | 0.0016 | 0.0038 |
None | 0.0105 | 0.0988 | 0.0525 | 0.0084 | 0.1057 | 0.011 | 0.014 | 0.1217 | 0.0155 | 0.0008 | 0.0095 | 0.1157 | 0.0323 | 0.0336 | 0.0238 | 0.0105 | 0.0216 | 0.0572 |
Nuclear Periphery | 0.027 | 0.012 | 0.0188 | 0.0034 | 0.016 | 0.0121 | 0.0608 | 0.0142 | 0.0182 | 0.0053 | 0.0039 | 0.0122 | 0.0099 | 0.018 | 0.0183 | 0.004 | 0.0083 | 0.0132 |
Nucleolus | 0.0053 | 0.0029 | 0.0028 | 0.0049 | 0.0009 | 0.0045 | 0.0031 | 0.0017 | 0.0007 | 0.0003 | 0.0009 | 0.0008 | 0.0016 | 0.0079 | 0.0016 | 0.0005 | 0.0005 | 0.0009 |
Nucleus | 0.0356 | 0.011 | 0.0173 | 0.0072 | 0.0027 | 0.02 | 0.0549 | 0.0054 | 0.0053 | 0.0012 | 0.0039 | 0.0053 | 0.0079 | 0.0105 | 0.0059 | 0.0033 | 0.0017 | 0.005 |
Peroxisomes | 0.0043 | 0.0004 | 0.0145 | 0.0076 | 0.0059 | 0.0008 | 0.0297 | 0.0006 | 0.0005 | 0.1286 | 0.0005 | 0.0022 | 0.0004 | 0.0004 | 0.0011 | 0.0005 | 0.0005 | 0.0005 |
Punctate Nuclear | 0.0033 | 0.0011 | 0.0046 | 0.0022 | 0.0019 | 0.0015 | 0.0018 | 0.0013 | 0.0004 | 0.0005 | 0.001 | 0.0005 | 0.0009 | 0.0006 | 0.0007 | 0.0002 | 0.0002 | 0.0004 |
Vacuole | 0.4033 | 0.3691 | 0.2798 | 0.3207 | 0.2603 | 0.405 | 0.3218 | 0.2935 | 0.3038 | 0.3279 | 0.2332 | 0.3088 | 0.3546 | 0.4001 | 0.3059 | 0.3506 | 0.3882 | 0.3787 |
Vacuole Periphery | 0.0763 | 0.0607 | 0.0595 | 0.0776 | 0.0951 | 0.0927 | 0.036 | 0.0484 | 0.07 | 0.1503 | 0.0335 | 0.1018 | 0.0719 | 0.0768 | 0.0776 | 0.0788 | 0.1003 | 0.1048 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.527 | 11.2821 | 10.3651 | 16.0359 | 19.48 | 6.0065 | 22.4072 | 24.7473 | 20.86 | 24.0273 |
Translational Efficiency | 0.271 | 0.4904 | 0.4438 | 0.2362 | 0.2489 | 0.9277 | 0.2934 | 0.2201 | 0.2193 | 0.2784 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
125 | 881 | 1751 | 435 | 2059 | 1923 | 1654 | 1462 | 2184 | 2804 | 3405 | 1897 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 899.13 | 847.13 | 947.17 | 923.59 | 783.15 | 857.89 | 789.72 | 860.93 | 789.79 | 854.51 | 870.69 | 875.30 |
Standard Deviation | 160.45 | 126.20 | 126.81 | 109.30 | 98.30 | 113.01 | 140.22 | 115.62 | 106.35 | 117.42 | 154.96 | 117.20 |
Intensity Change Log 2 | — | -0.085946 | 0.075094 | 0.038723 | — | 0.131504 | 0.012053 | 0.136607 | — | 0.019371 | 0.046089 | 0.085118 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000551 | 0.000066 | 0.000085 | 0.000270 | 0.000022 | 0.000037 | 0.000116 | 0.000437 | 0.000052 | 0.000046 | 0.000100 | 0.000399 |
Bud Neck | 0.006496 | 0.004457 | 0.008091 | 0.010742 | 0.003890 | 0.003959 | 0.015952 | 0.012158 | 0.004039 | 0.004116 | 0.011909 | 0.011833 |
Bud Site | 0.036358 | 0.010211 | 0.005859 | 0.028443 | 0.004390 | 0.006475 | 0.009737 | 0.022363 | 0.006219 | 0.007649 | 0.007743 | 0.023757 |
Cell Periphery | 0.000214 | 0.000151 | 0.000067 | 0.000050 | 0.000150 | 0.000139 | 0.000062 | 0.000104 | 0.000154 | 0.000143 | 0.000064 | 0.000091 |
Cytoplasm | 0.181705* | 0.020115 | 0.016581 | 0.308324* | 0.029053 | 0.013695 | 0.333917* | 0.388422* | 0.037790 | 0.015712 | 0.170729* | 0.370055* |
Cytoplasmic Foci | 0.093973 | 0.034836 | 0.008937 | 0.042540 | 0.034002 | 0.035729 | 0.022225 | 0.053020 | 0.037435 | 0.035449 | 0.015392 | 0.050617 |
Eisosomes | 0.000092 | 0.000016 | 0.000019 | 0.000028 | 0.000014 | 0.000017 | 0.000025 | 0.000038 | 0.000019 | 0.000016 | 0.000022 | 0.000036 |
Endoplasmic Reticulum | 0.002641 | 0.004771 | 0.004830 | 0.002065 | 0.010735 | 0.006407 | 0.004403 | 0.001844 | 0.010272 | 0.005893 | 0.004623 | 0.001895 |
Endosome | 0.043550 | 0.105264 | 0.042474 | 0.024504 | 0.086437 | 0.123072 | 0.027615 | 0.024715 | 0.083982 | 0.117477 | 0.035256 | 0.024667 |
Golgi | 0.020750 | 0.005957 | 0.003036 | 0.001767 | 0.002305 | 0.006833 | 0.000576 | 0.003677 | 0.003360 | 0.006558 | 0.001841 | 0.003239 |
Lipid Particles | 0.004450 | 0.002921 | 0.001464 | 0.001683 | 0.003548 | 0.003713 | 0.002164 | 0.002584 | 0.003600 | 0.003464 | 0.001804 | 0.002377 |
Mitochondria | 0.004666 | 0.005457 | 0.003046 | 0.000877 | 0.006222 | 0.013031 | 0.001553 | 0.003756 | 0.006133 | 0.010651 | 0.002321 | 0.003096 |
Mitotic Spindle | 0.001476 | 0.004493 | 0.006751 | 0.019032 | 0.000674 | 0.002255 | 0.007339 | 0.020036 | 0.000720 | 0.002958 | 0.007037 | 0.019806 |
None | 0.000222 | 0.000277 | 0.000895 | 0.001447 | 0.000379 | 0.000265 | 0.003180 | 0.002137 | 0.000370 | 0.000268 | 0.002005 | 0.001979 |
Nuclear Periphery | 0.000932 | 0.000987 | 0.004529 | 0.001694 | 0.000734 | 0.001206 | 0.003888 | 0.001532 | 0.000745 | 0.001138 | 0.004218 | 0.001569 |
Nuclear Periphery Foci | 0.001593 | 0.001779 | 0.002854 | 0.002546 | 0.000278 | 0.000698 | 0.001842 | 0.001582 | 0.000353 | 0.001038 | 0.002363 | 0.001803 |
Nucleolus | 0.000741 | 0.001326 | 0.012786 | 0.000948 | 0.000694 | 0.000917 | 0.010660 | 0.001033 | 0.000697 | 0.001046 | 0.011753 | 0.001013 |
Nucleus | 0.086446 | 0.003636 | 0.039002 | 0.058510 | 0.005117 | 0.002809 | 0.186307* | 0.066869 | 0.009772 | 0.003069 | 0.110557 | 0.064952 |
Peroxisomes | 0.002697 | 0.002273 | 0.001331 | 0.005311 | 0.000632 | 0.001043 | 0.000902 | 0.001640 | 0.000750 | 0.001429 | 0.001123 | 0.002482 |
Vacuole | 0.485415* | 0.489812* | 0.528283* | 0.487242* | 0.649123* | 0.472711* | 0.359087* | 0.388158* | 0.639753* | 0.478084* | 0.446095* | 0.410879* |
Vacuole Periphery | 0.025032 | 0.301195* | 0.309079* | 0.001977 | 0.161602* | 0.304989* | 0.008448 | 0.003895 | 0.153785* | 0.303797* | 0.163046* | 0.003455 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.86 | 1.75 | 1.19 | -3.40 | -2.87 | -1.60 | -8.20 | -2.77 | -2.61 | -1.66 | 0.05 | -3.96 | -2.93 | -2.97 | -2.15 |
Bud Neck | 0.87 | -1.85 | -1.81 | -3.30 | -0.29 | -0.11 | -10.20 | -6.21 | -5.89 | 4.21 | -0.23 | -10.50 | -6.83 | -6.68 | 2.38 |
Bud Site | 2.20 | 2.61 | 1.66 | -1.33 | -2.58 | -2.47 | -3.86 | -6.72 | -5.16 | -3.60 | -1.76 | -1.53 | -5.85 | -4.96 | -5.15 |
Cell Periphery | 0.60 | 2.59 | 3.05 | 6.21 | 3.64 | 0.42 | 4.51 | 3.28 | 3.42 | -2.16 | 0.38 | 4.58 | 3.96 | 5.26 | -1.11 |
Cytoplasm | 8.17 | 8.30 | -6.07 | -22.83 | -23.14 | 7.36 | -40.93 | -46.18 | -49.06 | -3.80 | 9.17 | -26.87 | -48.41 | -53.69 | -24.10 |
Cytoplasmic Foci | 5.13 | 8.27 | 5.58 | 1.23 | -8.18 | -0.83 | 7.09 | -3.66 | -2.68 | -10.79 | 0.55 | 13.41 | -1.36 | -2.01 | -16.05 |
Eisosomes | 7.88 | 7.62 | 6.35 | -5.49 | -5.03 | -0.66 | -8.00 | -12.84 | -5.19 | -5.79 | 0.75 | -2.87 | -10.12 | -6.75 | -8.51 |
Endoplasmic Reticulum | -2.49 | -4.28 | 0.95 | 3.46 | 8.57 | 4.20 | 7.57 | 10.79 | 7.61 | 10.76 | 4.73 | 7.07 | 10.78 | 8.33 | 13.78 |
Endosome | -9.93 | -1.33 | 4.55 | 17.03 | 11.60 | -7.61 | 20.88 | 23.90 | 26.00 | 4.59 | -8.28 | 17.57 | 24.69 | 30.92 | 12.03 |
Golgi | 1.12 | 1.46 | 1.73 | 5.41 | 4.29 | -6.40 | 5.34 | 0.53 | 5.57 | -2.35 | -4.51 | 2.13 | 1.87 | 7.00 | 0.28 |
Lipid Particles | 1.00 | 2.86 | 2.70 | 2.40 | -0.83 | -0.29 | 3.57 | 3.03 | 2.73 | -0.18 | 0.11 | 4.34 | 3.60 | 3.30 | -1.02 |
Mitochondria | -0.88 | 0.18 | 1.44 | 7.52 | 8.55 | -5.03 | 6.63 | 4.39 | 8.42 | -1.28 | -4.31 | 5.36 | 5.22 | 9.50 | 1.10 |
Mitotic Spindle | -2.36 | -4.58 | -4.34 | -3.09 | -2.52 | -2.22 | -5.14 | -6.95 | -6.03 | -3.33 | -3.51 | -7.54 | -8.18 | -6.63 | -4.27 |
None | -0.83 | -2.02 | -5.91 | -5.51 | -2.25 | 0.53 | -8.12 | -3.61 | -4.36 | 2.07 | 0.49 | -5.28 | -4.36 | -5.45 | -0.20 |
Nuclear Periphery | -0.20 | -6.23 | -5.73 | -5.10 | 3.86 | -2.46 | -7.68 | -6.63 | -2.95 | 5.06 | -2.52 | -9.76 | -8.23 | -4.73 | 6.43 |
Nuclear Periphery Foci | -0.19 | -2.33 | -3.11 | -1.59 | 0.34 | -3.23 | -8.42 | -9.78 | -4.82 | 2.72 | -3.01 | -7.32 | -12.05 | -3.24 | 2.52 |
Nucleolus | -2.27 | -11.45 | -2.06 | 1.14 | 11.10 | -1.43 | -9.28 | -1.83 | -0.07 | 9.04 | -2.37 | -14.65 | -2.21 | 0.80 | 14.35 |
Nucleus | 5.43 | 2.79 | 0.06 | -14.88 | -6.83 | 3.22 | -35.53 | -25.82 | -27.00 | 18.54 | 5.73 | -31.23 | -26.32 | -30.78 | 8.18 |
Peroxisomes | -0.07 | 1.65 | 0.05 | 0.09 | -0.63 | -2.10 | -2.12 | -3.01 | -0.14 | -1.85 | -2.46 | -3.04 | -1.98 | 0.26 | -0.74 |
Vacuole | 1.63 | 0.30 | 0.91 | -1.10 | 1.25 | 22.41* | 32.35* | 24.14 | 5.15 | -6.50 | 23.02 | 27.29 | 23.06 | 4.22 | 0.10 |
Vacuole Periphery | -22.78* | -25.36* | 1.96 | 36.60* | 47.33* | -21.70* | 41.00* | 42.30* | 55.55* | 7.40 | -25.83 | -1.19 | 41.46 | 66.47* | 37.74* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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