Standard name
Human Ortholog
Description Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0.05 0.07 0.1 0.21 0.18 0.16 0.05 0.07 0.06 0 0 0 0 0 0
Cytoplasm 0.21 0.31 0.07 0.26 0.36 0.48 0.44 0.45 0.52 0.68 0.67 0.13 0.1 0.09 0.19 0.19 0.06 0.15 0.08 0.06
Endoplasmic Reticulum 0.69 0.65 0.75 0.55 0.48 0.46 0.49 0.48 0.33 0.25 0.26 0.66 0.74 0.78 0.48 0.48 0.63 0.46 0.51 0.58
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0
Golgi 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.07 0 0.06 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0.05 0.14 0.09 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.09 0.08 0 0 0 0 0 0 0.09 0.07 0.07 0.16 0.16 0.12 0.19 0.19 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 2 0 2 2 0 0 0 0 5 3 2 1 1 4
Bud 4 0 2 2 1 1 6 9 8 8 12 3 2 0 2 1 0 5 5 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 3 2 1 4 14 26 41 84 63 67 6 18 17 0 0 0 1 1 2
Cytoplasm 39 31 11 34 66 146 164 189 212 236 276 17 27 25 72 34 13 29 19 17
Endoplasmic Reticulum 131 66 112 71 89 139 179 205 133 87 109 84 205 223 185 89 138 88 120 154
Endosome 0 0 0 0 0 0 0 0 0 1 0 0 0 0 18 9 10 6 10 6
Golgi 2 0 7 5 1 0 1 1 1 1 0 7 8 7 13 5 16 5 13 9
Mitochondria 0 0 0 0 0 2 1 1 0 1 0 0 0 0 0 0 0 0 1 0
Nucleus 1 0 0 0 1 1 1 0 1 0 1 0 0 0 4 1 0 5 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 2 1 1 6 2
Nucleolus 13 5 21 11 10 6 2 7 3 1 3 8 12 9 0 0 1 1 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 0 1 0
Vac/Vac Membrane 5 2 6 12 15 9 13 6 16 4 7 12 20 19 62 30 26 35 46 52
Unique Cell Count 190 101 149 129 184 303 369 424 405 349 413 127 276 287 389 187 222 190 237 268
Labelled Cell Count 197 107 161 136 188 318 395 459 460 404 475 137 292 300 389 187 222 190 237 268


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 135.3 150.7 99.2 92.5 83.5 77.6 74.8 76.5 69.5 69.4 68.4 105.3 98.5 99.6 109.8 117.8 123.8
Std Deviation (1e-4) 37.3 43.0 25.7 30.7 29.3 28.0 24.9 23.7 23.0 21.7 18.6 31.1 28.6 28.4 44.2 37.4 42.2
Intensity Change (Log2) -0.1 -0.25 -0.35 -0.41 -0.38 -0.51 -0.52 -0.54 0.09 -0.01 0.01 0.15 0.25 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.8 2.6 3.3 5.6 5.1 4.7 0 2.4 2.2
Cytoplasm 4.3 6.1 8.6 8.1 8.2 9.6 12.3 12.4 1.6 0.8 0.5
Endoplasmic Reticulum -3.5 -5.0 -5.9 -5.5 -5.7 -8.9 -10.5 -10.4 -1.6 -0.2 0.6
Endosome 0 0 0 0 0 0 0 0 0 0 0
Golgi -0.3 0 0 0 0 0 0 0 0.3 -1.0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.5 -2.7 -5.1 -6.8 -6.1 -6.8 -6.9 -6.9 -2.1 -3.6 -4.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.8 1.5 0 -0.3 0 0 0 0 1.8 1.3 1.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 141.7696 175.8522 153.7092 147.3512 152.0149 160.6683 128.3258 163.6114 144.7829 132.5702 139.054 154.277 105.9007 153.7149 122.1493 99.9632 92.7311 143.9824
Actin 0 0 0 0 0 0 0.0007 0 0 0 0 0 0.0071 0.0002 0.0003 0.1038 0.0551 0.0001
Bud 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0009 0.0003 0.0005 0.0037 0.0021 0.0003
Bud Neck 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001 0.0003 0.0003 0.0006 0.0002 0.0048 0.001 0.0004
Bud Periphery 0.0004 0.0003 0.0004 0.0002 0.0003 0.0007 0.0006 0.0006 0.0006 0.0004 0.0003 0.0021 0.0014 0.0012 0.0007 0.0089 0.001 0.0018
Bud Site 0.0001 0.0002 0.0001 0 0 0 0.0002 0.0001 0.0001 0.0001 0 0.0002 0.0017 0.0029 0.0007 0.0145 0.0009 0.0002
Cell Periphery 0.3781 0.4185 0.43 0.3777 0.4034 0.4012 0.3371 0.3896 0.3809 0.4208 0.2934 0.382 0.2342 0.3827 0.3337 0.297 0.1684 0.3035
Cytoplasm 0.0046 0.0137 0.0011 0.0019 0.0006 0.0216 0.0043 0.0091 0.0037 0.0011 0.0015 0.0021 0.0468 0.0015 0.0415 0.0146 0.1137 0.0013
Cytoplasmic Foci 0.0002 0.0001 0.0028 0.0001 0 0.0003 0.0022 0.0004 0.0002 0 0.0001 0.0002 0.0231 0.0003 0.0042 0.0015 0.0396 0.0004
Eisosomes 0.0001 0.0006 0 0.0001 0 0.0001 0.0036 0.0003 0 0 0 0.0001 0.0002 0.0002 0.0001 0.0012 0 0.0001
Endoplasmic Reticulum 0.0027 0.0038 0.0011 0.0023 0.001 0.0034 0.0039 0.0056 0.0069 0.0069 0.0007 0.0059 0.0306 0.0021 0.0063 0.0076 0.0048 0.001
Endosome 0.001 0.0003 0.0006 0.0003 0.0003 0.0017 0.0033 0.0011 0.0013 0.0005 0.0024 0.0019 0.019 0.0007 0.0141 0.0159 0.1413 0.0016
Golgi 0.0001 0.0001 0.0001 0 0 0.0003 0.0025 0.0002 0.0002 0 0.0002 0.0003 0.0042 0.0004 0.0007 0.0043 0.0216 0.0008
Lipid Particles 0.0187 0.0201 0.0087 0.0143 0.006 0.0254 0.028 0.0545 0.019 0.0087 0.0052 0.0317 0.0748 0.0379 0.0097 0.0041 0.0016 0.0624
Mitochondria 0.0002 0.0006 0.0001 0 0 0.0001 0.0029 0.0003 0.0001 0 0.0001 0.0001 0.0029 0.0002 0.0013 0.0009 0.0057 0.0002
None 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0011 0.0001 0 0 0 0.0001 0.0054 0 0.0018 0.0002 0.0006 0.0001
Nuclear Periphery 0.0056 0.0031 0.0012 0.0095 0.0011 0.0046 0.01 0.0033 0.0028 0.0021 0.0012 0.01 0.049 0.0088 0.0076 0.0058 0.0026 0.0035
Nucleolus 0.0059 0.0034 0.0056 0.0026 0.001 0.0034 0.01 0.0032 0.0015 0.0025 0.0011 0.004 0.0189 0.0067 0.0244 0.0019 0.0029 0.0156
Nucleus 0.0033 0.0012 0.0011 0.001 0.0006 0.002 0.004 0.0011 0.0009 0.0008 0.0011 0.0026 0.0103 0.0018 0.007 0.0036 0.0026 0.0074
Peroxisomes 0 0 0.0001 0 0 0 0.0003 0 0 0 0 0 0.0008 0 0.0002 0.0002 0.0044 0
Punctate Nuclear 0.0001 0 0.001 0 0 0 0.0004 0 0 0 0 0 0.0076 0 0.0002 0.0002 0.0012 0
Vacuole 0.5592 0.5256 0.5379 0.5776 0.5723 0.5159 0.5451 0.5061 0.5523 0.5357 0.6597 0.5132 0.4007 0.5232 0.5169 0.4849 0.3832 0.5427
Vacuole Periphery 0.0192 0.0083 0.0079 0.012 0.0132 0.0188 0.0393 0.0241 0.0293 0.02 0.0328 0.0429 0.0602 0.0284 0.0281 0.0204 0.0456 0.0565

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 331.9184 473.1398 1100.8759 1442.6403 366.3763 475.1875 822.995 1324.3626 1036.9597 642.9027
Translational Efficiency 1.8961 2.5801 2.2041 0.7969 2.4387 1.8708 2.0597 1.6781 2.0696 1.8685

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication
Localization
Cell Percentages ER (70%)
Cell Cycle Regulation No
Subcompartmental Group ER-4

Pmp1

Pmp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pmp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available