Standard name
Human Ortholog
Description Mitochondrial asparaginyl-tRNA synthetase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0 0
Cytoplasm 0 0.13 0.06 0.13 0.11 0 0.12 0 0 0.05 0 0.06 0.2 0.29 0.33 0.12 0.23 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.12 0.05 0 0 0 0 0 0 0
Endosome 0 0.08 0 0 0.08 0 0 0 0 0 0 0 0 0.12 0.11 0.29 0.15 0.35 0.1 0.13 0.14 0.09 0.09 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.22 0.18 0 0 0 0 0.05 0
Mitochondria 0.96 0.66 0.9 0.87 0.8 0.94 0.82 0.93 0.97 0.96 0.95 0.96 0.7 0.57 0.46 0.29 0.13 0.06 0.7 0.64 0.67 0.77 0.57 0.57
Nucleus 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0.06 0.25 0 0.24 0 0 0.05 0 0 0
SpindlePole 0 0.06 0 0 0 0 0 0.09 0 0 0 0 0 0 0.05 0 0.12 0.12 0 0 0 0 0 0.06
Vac/Vac Membrane 0.09 0.14 0 0.05 0.07 0.09 0.05 0 0 0 0 0 0.07 0.17 0.13 0.08 0.18 0.18 0 0.06 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 2 7 3 0 5 4
Bud 6 5 4 0 5 1 2 7 2 8 5 5 2 0 1 1 0 3 0 0 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1 2
Bud Site 0 0 0 0 0 1 1 7 0 3 3 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 0 1 0 0 0 2 0 1 2 0 2 1 1 6 1 0 1 0 0 0 0
Cytoplasm 8 20 7 8 14 4 20 6 1 11 1 10 16 28 32 3 14 1 1 1 0 0 1 0
Endoplasmic Reticulum 8 7 5 0 0 1 3 0 3 1 2 1 6 2 2 3 3 0 2 1 1 0 1 1
Endosome 1 13 3 0 10 1 1 3 0 0 1 1 1 12 11 7 9 6 19 29 15 4 8 7
Golgi 0 2 1 2 0 0 1 0 0 0 0 0 0 1 0 1 13 3 4 6 2 0 5 0
Mitochondria 183 105 104 53 99 133 140 166 115 195 149 164 57 56 45 7 8 1 135 140 75 37 55 47
Nucleus 0 0 0 0 2 3 9 4 2 5 2 1 0 1 0 1 2 0 0 1 0 0 0 1
Nuclear Periphery 0 1 1 0 1 2 4 1 8 3 2 4 0 0 0 0 0 0 1 1 0 0 0 0
Nucleolus 3 0 1 1 2 1 6 4 5 2 0 4 0 0 0 0 0 0 0 1 0 0 1 0
Peroxisomes 0 6 3 0 9 1 3 1 0 0 0 1 2 2 6 6 1 4 6 5 5 1 3 1
SpindlePole 4 10 4 1 4 1 7 16 0 2 5 4 1 1 5 1 7 2 6 3 2 0 2 4
Vac/Vac Membrane 18 22 5 3 8 12 9 6 3 8 6 5 6 17 13 2 11 3 8 12 2 2 4 4
Unique Cell Count 191 159 115 61 123 141 171 178 119 204 157 171 82 98 97 24 60 17 194 220 114 49 97 83
Labelled Cell Count 231 193 138 68 155 161 206 222 141 238 177 202 92 122 117 33 75 24 194 220 114 49 97 83


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.7 4.1 4.4 4.5 3.9 3.8 3.8 3.6 3.5 3.5 3.5 4.7 4.7 4.6 6.5 7.1 6.8 4.5 5.1 5.6
Std Deviation (1e-4) 0.6 0.9 0.7 1.7 1.7 0.7 2.0 1.4 0.7 1.2 0.9 1.2 0.9 1.0 1.1 2.5 2.3 2.2 1.0 1.7 1.9
Intensity Change (Log2) – – – 0.09 0.13 -0.07 -0.11 -0.1 -0.18 -0.25 -0.22 -0.22 0.2 0.19 0.17 0.66 0.79 0.74 0.12 0.3 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 3.0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0728 0.0106 0.0317 0.0121 0.0277 0.0245 0.0465 0.0411 0.0322 0.0587 0.001 0.016 0.133 0.0721 0.0291 0.0439 0.0049 0.0969
Bud 0.0015 0.0032 0.0027 0.0017 0.005 0.001 0.0028 0.0025 0.0015 0.0022 0.0002 0.0008 0.0017 0.0025 0.0016 0.0002 0.0011 0.0028
Bud Neck 0.0151 0.0017 0.0018 0.0201 0.0016 0.0081 0.0026 0.0007 0.0087 0.0074 0.0004 0.0062 0.004 0.0042 0.0012 0.0002 0.0008 0.0023
Bud Periphery 0.0026 0.0065 0.009 0.0053 0.0172 0.0019 0.007 0.0033 0.003 0.0088 0.0005 0.0048 0.0029 0.0056 0.0026 0.0009 0.0012 0.007
Bud Site 0.0232 0.0383 0.0108 0.0034 0.0074 0.0035 0.0067 0.0347 0.0208 0.0023 0.0005 0.003 0.0225 0.0287 0.0066 0.0006 0.0017 0.005
Cell Periphery 0.0017 0.0013 0.0019 0.0014 0.0019 0.0007 0.0031 0.0009 0.0018 0.0025 0.0001 0.0023 0.002 0.0058 0.0005 0.0011 0.0001 0.0013
Cytoplasm 0.0142 0.0184 0.0345 0.0134 0.0128 0.0249 0.0108 0.0083 0.0154 0.0294 0.0003 0.0164 0.0095 0.0215 0.0231 0.0011 0.0045 0.0054
Cytoplasmic Foci 0.0764 0.0661 0.0603 0.0594 0.0351 0.0626 0.0794 0.0561 0.065 0.0659 0.0264 0.0523 0.1382 0.1175 0.0811 0.0398 0.256 0.047
Eisosomes 0.0016 0.0005 0.0013 0.0004 0.001 0.0003 0.0015 0.0011 0.0021 0.0027 0.0001 0.002 0.0015 0.0026 0.0003 0.0015 0.0001 0.0022
Endoplasmic Reticulum 0.0142 0.0032 0.004 0.0112 0.0031 0.0019 0.0053 0.0016 0.0021 0.0046 0.0001 0.0053 0.0063 0.0034 0.0082 0.0013 0.0003 0.0093
Endosome 0.1611 0.1705 0.1201 0.12 0.0151 0.1023 0.2042 0.1785 0.0841 0.0861 0.0453 0.1343 0.1217 0.1644 0.1378 0.0119 0.1744 0.0825
Golgi 0.0644 0.0757 0.0689 0.0493 0.0075 0.0686 0.0559 0.0842 0.0741 0.0555 0.0548 0.0478 0.1722 0.1042 0.0521 0.0191 0.0898 0.0984
Lipid Particles 0.0739 0.0322 0.0413 0.0248 0.009 0.0441 0.0363 0.0319 0.0122 0.0317 0.0079 0.0945 0.064 0.0472 0.0591 0.0089 0.02 0.0526
Mitochondria 0.3578 0.4758 0.5001 0.6182 0.656 0.5301 0.3252 0.4704 0.5737 0.4775 0.5723 0.48 0.1571 0.2451 0.4194 0.7783 0.1225 0.5246
None 0.013 0.0197 0.019 0.0062 0.0734 0.0301 0.0175 0.0353 0.056 0.0387 0.0002 0.0687 0.0071 0.0788 0.0406 0.0007 0.0016 0.0035
Nuclear Periphery 0.0105 0.0014 0.0065 0.0016 0.0249 0.0005 0.0095 0.0009 0.0005 0.001 0.0001 0.003 0.0308 0.001 0.0064 0.0002 0.0002 0.021
Nucleolus 0.0031 0.0007 0.0018 0.0012 0.0014 0.0042 0.001 0.001 0.0006 0.0009 0.0001 0.0007 0.0009 0.0019 0.0005 0 0.0002 0.0006
Nucleus 0.0056 0.0009 0.0051 0.0011 0.0542 0.0009 0.0641 0.0007 0.0006 0.001 0.0001 0.0009 0.0132 0.0011 0.0011 0.0001 0.0002 0.0023
Peroxisomes 0.0601 0.0383 0.0573 0.0334 0.0329 0.0793 0.0609 0.0205 0.0326 0.0967 0.2861 0.0442 0.0906 0.0752 0.1097 0.0875 0.3151 0.0202
Punctate Nuclear 0.0048 0.0004 0.0019 0.0008 0.0017 0.001 0.0027 0.0004 0.0004 0.0019 0.0001 0.0015 0.0124 0.0021 0.0068 0.0007 0.0011 0.0064
Vacuole 0.0123 0.0231 0.0126 0.0086 0.0084 0.0052 0.0509 0.0171 0.0084 0.0149 0.0016 0.0082 0.0043 0.0118 0.0064 0.0007 0.0023 0.005
Vacuole Periphery 0.0102 0.0115 0.0073 0.0062 0.0028 0.0042 0.0061 0.0088 0.0042 0.0094 0.0017 0.0072 0.0041 0.0033 0.0056 0.001 0.0019 0.0037

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.5954 4.6684 9.8977 8.6798 2.4182 8.202 9.1034 13.4261 11.7597 9.4003
Translational Efficiency 0.5127 1.1078 0.6044 0.5609 1.3106 0.5698 0.8545 0.4646 0.5998 0.5575

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1256 797 579 1134 674 155 183 130 1930 952 762 1264

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 671.35 722.50 881.48 829.24 675.52 728.02 941.70 844.73 672.81 723.40 895.94 830.83
Standard Deviation 72.83 100.42 98.41 107.26 81.12 80.02 89.97 121.42 75.85 97.41 99.82 108.90
Intensity Change Log 2 — 0.105932 0.392863 0.304725 — 0.107980 0.479269 0.322492 — 0.106960 0.436846 0.313663

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001003 0.001999 0.001255 0.027791 0.000770 0.001769 0.002068 0.008204 0.000922 0.001961 0.001450 0.025777
Bud Neck 0.052276 0.023899 0.019430 0.017747 0.032445 0.052296 0.022159 0.020251 0.045350 0.028522 0.020086 0.018005
Bud Site 0.020340 0.024540 0.030943 0.094109 0.017698 0.036041 0.032325 0.041377 0.019417 0.026413 0.031275 0.088686
Cell Periphery 0.000331 0.000363 0.000143 0.000363 0.000285 0.000912 0.000157 0.000809 0.000315 0.000452 0.000147 0.000409
Cytoplasm 0.094988 0.041883 0.031918 0.060467 0.048446 0.039356 0.054305 0.062462 0.078734 0.041471 0.037295 0.060672
Cytoplasmic Foci 0.226848* 0.157166 0.163147 0.192092* 0.211466* 0.165325 0.224654* 0.172899 0.221476* 0.158495 0.177918 0.190118*
Eisosomes 0.000228 0.000223 0.000101 0.000262 0.000219 0.000252 0.000172 0.000211 0.000225 0.000228 0.000118 0.000257
Endoplasmic Reticulum 0.002409 0.000907 0.003503 0.004015 0.000999 0.000528 0.005393 0.004196 0.001916 0.000846 0.003957 0.004034
Endosome 0.094481 0.104731 0.155255 0.073379 0.096750 0.124989 0.149244 0.125910 0.095273 0.108029 0.153812 0.078781
Golgi 0.043776 0.142988* 0.077095 0.105895 0.094640 0.091752 0.050918 0.056024 0.061539 0.134646* 0.070808 0.100766
Lipid Particles 0.029207 0.010133 0.010191 0.004501 0.019905 0.009813 0.030039 0.046820 0.025959 0.010081 0.014958 0.008854
Mitochondria 0.251327* 0.326964* 0.118979* 0.132424* 0.314309* 0.269357* 0.062710 0.106506* 0.273322* 0.317585* 0.105465* 0.129759*
Mitotic Spindle 0.007997 0.019817 0.121414* 0.061464 0.010472 0.013374 0.069724 0.043444 0.008861 0.018768 0.109000* 0.059611
None 0.004204 0.001439 0.001064 0.000306 0.001333 0.000362 0.000588 0.000729 0.003202 0.001264 0.000950 0.000349
Nuclear Periphery 0.000802 0.000152 0.003320 0.000306 0.000330 0.000229 0.003813 0.002326 0.000637 0.000164 0.003438 0.000514
Nuclear Periphery Foci 0.000562 0.000378 0.003993 0.000682 0.000348 0.000747 0.006243 0.003548 0.000487 0.000438 0.004533 0.000977
Nucleolus 0.006102 0.002110 0.012091 0.000506 0.003517 0.004738 0.014756 0.007073 0.005199 0.002537 0.012731 0.001181
Nucleus 0.036464 0.010059 0.034259 0.009922 0.024959 0.015394 0.037068 0.044659 0.032446 0.010927 0.034933 0.013495
Peroxisomes 0.048828 0.089956 0.039710 0.108214* 0.080858 0.132210* 0.075436 0.088514 0.060014 0.096836 0.048290 0.106188*
Vacuole 0.060586 0.026719 0.133342 0.095319 0.025977 0.028664 0.128391 0.141918 0.048500 0.027036 0.132153 0.100111
Vacuole Periphery 0.017241 0.013573 0.038848 0.010235 0.014274 0.011893 0.029836 0.022117 0.016205 0.013300 0.036684 0.011457

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.57 -0.58 -9.30 -9.05 -9.35 -1.97 -2.61 -3.04 -2.57 -2.43 -2.27 -1.50 -9.64 -9.26 -9.48
Bud Neck 6.80 8.25 9.25 1.84 0.37 -1.94 2.25 2.43 3.15 0.53 4.71 8.24 9.41 3.30 0.67
Bud Site -1.56 -2.87 -14.56 -13.11 -10.74 -2.41 -2.51 -2.54 -0.43 -0.78 -2.75 -3.81 -15.06 -12.55 -10.82
Cell Periphery -0.68 5.89 -0.41 0.30 -5.42 -1.49 3.48 -0.93 0.20 -1.17 -1.74 6.72 -1.18 0.62 -3.87
Cytoplasm 8.15 9.92 4.93 -4.05 -5.83 1.06 -0.71 -1.13 -1.63 -0.57 7.08 7.92 2.67 -4.47 -5.18
Cytoplasmic Foci 8.73 7.89 5.12 -3.86 -3.15 2.58 -0.60 2.25 -0.22 2.38 9.01 6.41 5.32 -3.65 -1.31
Eisosomes 0.13 7.06 -1.03 -0.98 -6.92 -0.95 1.76 -0.17 0.73 -1.56 -0.15 7.62 -1.21 -0.83 -6.50
Endoplasmic Reticulum 3.83 -2.67 -2.39 -4.72 -0.56 2.00 -3.97 -4.45 -5.12 0.91 3.75 -4.97 -3.55 -5.36 -0.03
Endosome -1.56 -7.60 4.96 5.75 11.36 -1.84 -4.09 -1.42 0.21 1.38 -2.13 -8.61 4.49 5.62 11.79
Golgi -13.69 -5.24 -10.10 5.23 -3.43 0.27 5.45 3.51 2.43 -0.42 -11.08 -1.30 -6.29 5.31 -4.22
Lipid Particles 8.03 7.88 12.51 3.71 3.81 2.42 -1.37 -2.50 -3.36 -1.50 8.00 4.72 8.82 0.68 2.71
Mitochondria -5.28 11.81 11.80 15.05 -0.96 1.70 16.72 10.18 5.52 -2.21 -3.48 18.06 16.11 15.86 -2.37
Mitotic Spindle -3.71 -12.34 -11.46 -8.16 5.75 -0.82 -5.86 -2.62 -2.30 1.93 -3.75 -13.53 -11.78 -8.66 5.73
None 2.85 3.27 4.23 3.20 3.49 4.40 3.28 1.71 -1.81 -0.79 2.94 3.52 4.68 3.02 3.47
Nuclear Periphery 4.80 -3.69 2.84 -3.77 4.35 0.68 -3.65 -2.86 -2.95 1.14 5.03 -4.92 0.37 -4.51 5.00
Nuclear Periphery Foci 1.68 -4.61 -1.35 -2.52 4.33 -0.97 -3.97 -3.67 -2.84 1.53 0.40 -6.05 -3.67 -3.41 5.14
Nucleolus 4.08 -3.04 6.01 3.70 6.58 -0.35 -3.06 -1.68 -0.97 1.65 3.38 -4.39 5.62 2.02 7.14
Nucleus 7.35 -0.02 7.55 -0.85 5.15 0.98 -2.18 -2.13 -2.51 -0.37 6.82 -1.25 5.90 -1.77 5.31
Peroxisomes -6.26 1.79 -9.38 -1.86 -10.18 -2.89 0.26 -0.34 2.07 -0.47 -5.85 2.30 -7.61 -0.82 -8.77
Vacuole 7.99 -10.19 -8.09 -13.91 2.73 -0.55 -8.64 -6.21 -5.91 -0.98 6.44 -13.83 -11.62 -15.13 2.29
Vacuole Periphery 2.06 -6.50 4.88 2.54 9.22 0.74 -3.09 -1.47 -1.77 1.20 1.92 -7.35 3.97 1.68 9.47
Standard name
Human Ortholog
Description Mitochondrial asparaginyl-tRNA synthetase
Localization
Cell Percentages mitochondrion (69%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Slm5

Slm5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slm5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available