Standard name | |
---|---|
Human Ortholog | |
Description | Negative regulator of WASP-Arp23 complex; involved in endocytic site formation; directly inhibits Las17p stimulation of Arp23 complex-mediated actin assembly in vitro; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 93 | 76 | 163 | 115 | 142 | 126 | 174 | 120 | 102 | 106 | 88 | 128 | 174 | 173 | 175 | 93 | 99 | 87 | 81 | 78 | 109 | 75 | 94 | 77 |
Bud | 23 | 30 | 43 | 38 | 72 | 55 | 76 | 47 | 54 | 47 | 45 | 59 | 32 | 35 | 25 | 24 | 20 | 29 | 19 | 19 | 16 | 1 | 8 | 3 |
Bud Neck | 11 | 9 | 14 | 12 | 18 | 4 | 8 | 5 | 5 | 4 | 2 | 7 | 1 | 0 | 0 | 2 | 15 | 7 | 38 | 56 | 62 | 18 | 20 | 14 |
Bud Site | 43 | 35 | 39 | 36 | 40 | 51 | 37 | 15 | 20 | 22 | 10 | 25 | 34 | 13 | 15 | 15 | 15 | 19 | – | – | – | – | – | – |
Cell Periphery | 28 | 29 | 48 | 39 | 23 | 38 | 59 | 42 | 46 | 41 | 42 | 63 | 85 | 122 | 162 | 68 | 68 | 44 | 48 | 28 | 57 | 80 | 162 | 149 |
Cytoplasm | 19 | 7 | 16 | 19 | 20 | 21 | 22 | 29 | 7 | 13 | 11 | 16 | 9 | 7 | 23 | 16 | 14 | 17 | 0 | 0 | 3 | 0 | 1 | 0 |
Endoplasmic Reticulum | 3 | 1 | 8 | 5 | 1 | 6 | 8 | 17 | 5 | 9 | 7 | 8 | 5 | 4 | 10 | 2 | 6 | 8 | 3 | 3 | 8 | 4 | 8 | 4 |
Endosome | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 4 | 2 | 2 | 1 | 2 | 0 |
Golgi | 0 | 1 | 3 | 2 | 2 | 0 | 1 | 5 | 1 | 0 | 0 | 8 | 0 | 1 | 2 | 50 | 72 | 35 | 1 | 0 | 0 | 0 | 2 | 1 |
Mitochondria | 76 | 46 | 39 | 54 | 36 | 80 | 146 | 70 | 159 | 195 | 167 | 165 | 67 | 62 | 72 | 32 | 28 | 25 | 8 | 7 | 16 | 3 | 5 | 6 |
Nucleus | 6 | 4 | 24 | 6 | 8 | 3 | 11 | 11 | 3 | 7 | 2 | 9 | 1 | 1 | 0 | 1 | 6 | 6 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 3 | 0 | 0 | 0 |
Nucleolus | 5 | 3 | 1 | 5 | 6 | 4 | 10 | 6 | 7 | 4 | 2 | 4 | 0 | 0 | 0 | 9 | 8 | 6 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 54 | 33 | 21 | 23 | 16 | 50 | 56 | 36 | 35 | 26 | 24 | 37 | 115 | 136 | 142 | 22 | 25 | 20 | 2 | 1 | 3 | 0 | 0 | 1 |
SpindlePole | 0 | 2 | 13 | 7 | 18 | 7 | 7 | 11 | 1 | 4 | 1 | 5 | 0 | 1 | 0 | 9 | 8 | 22 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 6 | 1 | 2 | 0 | 6 | 8 | 6 | 7 | 3 | 7 | 0 | 0 | 0 | 4 | 3 | 5 | 3 | 4 | 5 | 1 | 1 | 0 |
Unique Cell Count | 190 | 152 | 256 | 198 | 231 | 242 | 327 | 247 | 241 | 271 | 223 | 287 | 256 | 273 | 325 | 220 | 218 | 203 | 221 | 204 | 293 | 192 | 310 | 264 |
Labelled Cell Count | 363 | 279 | 439 | 363 | 404 | 445 | 622 | 424 | 454 | 485 | 404 | 541 | 523 | 557 | 628 | 347 | 388 | 332 | 221 | 204 | 293 | 192 | 310 | 264 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.0 | 8.3 | 8.6 | 7.6 | 8.4 | 7.5 | 7.9 | 8.1 | 7.1 | 6.8 | 6.7 | 7.6 | 9.0 | 9.6 | 10.4 | 14.9 | 14.4 | 12.0 | 7.9 | 7.8 | 8.4 |
Std Deviation (1e-4) | 2.0 | 1.6 | 1.6 | 1.6 | 1.7 | 1.6 | 1.7 | 1.6 | 1.6 | 1.5 | 1.5 | 1.7 | 1.4 | 1.7 | 2.0 | 3.6 | 3.5 | 3.2 | 2.1 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.03 | -0.2 | -0.12 | -0.09 | -0.28 | -0.34 | -0.37 | -0.17 | 0.07 | 0.16 | 0.27 | 0.79 | 0.75 | 0.48 | -0.13 | -0.14 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.2 | -0.5 | -2.6 | -2.5 | -3.4 | -4.8 | -5.6 | -5.3 | -4.4 | 1.0 | -0.1 | -2.4 | -4.7 | -4.0 | -4.4 |
Bud | 0.7 | 3.7 | 1.7 | 1.9 | 0.7 | 1.6 | 0.2 | 1.0 | 1.1 | -1.4 | -1.3 | -3.4 | -1.8 | -2.4 | -0.7 |
Bud Neck | 0.3 | 1.0 | -2.3 | -1.9 | -2.0 | -2.0 | -2.5 | -2.8 | -1.8 | -3.4 | -3.9 | -4.3 | -2.8 | 0.6 | -1.0 |
Bud Site | 0.8 | 0.6 | 1.7 | -1.4 | -3.3 | -2.4 | -2.6 | -3.9 | -2.4 | -0.6 | -4.0 | -4.4 | -2.9 | -2.9 | -1.9 |
Cell Periphery | 0.3 | -2.7 | -0.9 | -0.2 | -0.5 | 0.1 | -1.1 | 0 | 0.9 | 3.7 | 6.4 | 7.7 | 3.1 | 3.1 | 0.8 |
Cytoplasm | 1.3 | 1.0 | 1.0 | 0.2 | 2.2 | -1.8 | -0.7 | -0.6 | -0.3 | -1.4 | -2.1 | 0.4 | 0.4 | 0.1 | 0.9 |
Endoplasmic Reticulum | -0.4 | 0 | -0.4 | -0.5 | 1.9 | -0.7 | 0.1 | 0 | -0.2 | -0.8 | -1.3 | 0 | 0 | -0.2 | 0.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 | 7.5 | 9.5 | 6.2 |
Mitochondria | 3.2 | 0.1 | 4.7 | 7.6 | 3.6 | 11.5 | 13.1 | 13.2 | 10.1 | 3.1 | 2.2 | 2.1 | -0.2 | -0.7 | -0.9 |
Nucleus | -2.7 | -2.6 | -4.0 | -3.0 | -2.2 | -4.0 | -3.3 | -4.1 | -3.0 | -4.7 | -4.9 | -5.6 | -4.3 | -3.0 | -2.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.2 | -0.5 | 4.0 | 3.2 | 2.3 | 2.2 | 0.6 | 1.0 | 1.8 | 9.4 | 10.5 | 9.5 | 0.7 | 1.2 | 0.6 |
SpindlePole | -0.8 | 1.2 | -1.2 | -1.9 | -0.3 | -3.1 | -2.3 | -3.0 | -2.2 | -3.7 | -3.4 | -4.1 | -0.5 | -0.7 | 2.3 |
Vacuole | 0 | 0 | 0 | -0.4 | 0.6 | 0.1 | 0.2 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.9403 | 7.8561 | 7.0583 | 5.697 | 5.5518 | 7.4041 | 5.1515 | 5.745 | 4.6819 | 4.4534 | 4.5507 | 5.2484 | 5.5879 | 6.8977 | 5.8104 | 5.2198 | 4.2837 | 6.1125 |
Actin | 0.2804 | 0.1295 | 0.1713 | 0.1521 | 0.4462 | 0.2561 | 0.2088 | 0.1379 | 0.1358 | 0.1497 | 0.2952 | 0.2116 | 0.1235 | 0.0582 | 0.0657 | 0.0496 | 0.0549 | 0.0882 |
Bud | 0.0035 | 0.0024 | 0.0029 | 0.0041 | 0.0031 | 0.0031 | 0.0037 | 0.0021 | 0.0035 | 0.0024 | 0.008 | 0.0041 | 0.0039 | 0.0018 | 0.0026 | 0.0013 | 0.006 | 0.0035 |
Bud Neck | 0.1107 | 0.043 | 0.4928 | 0.4462 | 0.2547 | 0.4867 | 0.0757 | 0.027 | 0.4623 | 0.4083 | 0.1973 | 0.4205 | 0.1031 | 0.0646 | 0.496 | 0.502 | 0.088 | 0.374 |
Bud Periphery | 0.0082 | 0.003 | 0.0151 | 0.0403 | 0.0298 | 0.0272 | 0.0107 | 0.0041 | 0.0083 | 0.0218 | 0.0754 | 0.0307 | 0.0121 | 0.0037 | 0.0078 | 0.0088 | 0.0888 | 0.0412 |
Bud Site | 0.3422 | 0.7516 | 0.173 | 0.0149 | 0.0086 | 0.0185 | 0.2555 | 0.7375 | 0.2431 | 0.0624 | 0.0436 | 0.0163 | 0.3067 | 0.7531 | 0.2412 | 0.0126 | 0.0179 | 0.0236 |
Cell Periphery | 0.0685 | 0.0089 | 0.038 | 0.1164 | 0.0865 | 0.0973 | 0.1271 | 0.0397 | 0.0397 | 0.1583 | 0.1447 | 0.1747 | 0.1484 | 0.0477 | 0.0456 | 0.0763 | 0.2876 | 0.198 |
Cytoplasm | 0.0049 | 0.0175 | 0.0107 | 0.0023 | 0.0015 | 0.0073 | 0.0056 | 0.0021 | 0.0035 | 0.0023 | 0.0026 | 0.0038 | 0.0077 | 0.0018 | 0.0037 | 0.0035 | 0.0071 | 0.0068 |
Cytoplasmic Foci | 0.013 | 0.0033 | 0.0162 | 0.0188 | 0.0013 | 0.0019 | 0.0162 | 0.0014 | 0.017 | 0.0028 | 0.0228 | 0.0013 | 0.0252 | 0.0012 | 0.0139 | 0.0036 | 0.0381 | 0.0262 |
Eisosomes | 0.1019 | 0.0346 | 0.0163 | 0.0313 | 0.1601 | 0.0887 | 0.2111 | 0.0282 | 0.0321 | 0.0959 | 0.1613 | 0.1229 | 0.1164 | 0.0358 | 0.0224 | 0.1687 | 0.0793 | 0.1207 |
Endoplasmic Reticulum | 0.0025 | 0.0001 | 0.0043 | 0.0026 | 0.0004 | 0.0027 | 0.0014 | 0.0002 | 0.0003 | 0.0024 | 0.0014 | 0.001 | 0.0048 | 0.0007 | 0.0016 | 0.0264 | 0.0065 | 0.0045 |
Endosome | 0.0057 | 0.002 | 0.0048 | 0.0343 | 0.0003 | 0.001 | 0.0171 | 0.0021 | 0.0099 | 0.0208 | 0.0087 | 0.0014 | 0.0147 | 0.0029 | 0.0137 | 0.0089 | 0.0557 | 0.0144 |
Golgi | 0.0089 | 0.0006 | 0.0026 | 0.0185 | 0.0036 | 0.0017 | 0.0193 | 0.0041 | 0.0154 | 0.0172 | 0.0023 | 0.0036 | 0.0165 | 0.0009 | 0.0062 | 0.0154 | 0.0115 | 0.0042 |
Lipid Particles | 0.0143 | 0.0003 | 0.0139 | 0.0234 | 0.0006 | 0.0011 | 0.0164 | 0.0005 | 0.0054 | 0.004 | 0.0153 | 0.0007 | 0.0141 | 0.0012 | 0.0136 | 0.01 | 0.0786 | 0.0091 |
Mitochondria | 0.0055 | 0.0001 | 0.0111 | 0.0136 | 0.001 | 0.0005 | 0.0133 | 0.0006 | 0.0078 | 0.0419 | 0.0007 | 0.003 | 0.0313 | 0.0005 | 0.014 | 0.0239 | 0.0479 | 0.0104 |
None | 0.0026 | 0.0002 | 0.0042 | 0.0006 | 0.0002 | 0.0024 | 0.0009 | 0.0002 | 0.0006 | 0.0007 | 0.0005 | 0.0004 | 0.0053 | 0.0044 | 0.005 | 0.0639 | 0.0163 | 0.0022 |
Nuclear Periphery | 0.0013 | 0 | 0.0045 | 0.0041 | 0.0001 | 0.0004 | 0.0007 | 0.0002 | 0.0001 | 0.0023 | 0.0003 | 0.0002 | 0.0024 | 0.0005 | 0.0015 | 0.0118 | 0.0052 | 0.0008 |
Nucleolus | 0.0031 | 0.0001 | 0.0014 | 0.0017 | 0.0001 | 0.0005 | 0.0007 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0063 | 0.0027 | 0.0031 | 0.0009 | 0.0057 | 0.0089 |
Nucleus | 0.0017 | 0.0002 | 0.0048 | 0.0024 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0004 | 0.0006 | 0.0002 | 0.0004 | 0.0405 | 0.0108 | 0.0261 | 0.0027 | 0.0507 | 0.0487 |
Peroxisomes | 0.0134 | 0.0005 | 0.0085 | 0.0612 | 0.001 | 0.0008 | 0.0125 | 0.0004 | 0.0128 | 0.002 | 0.0183 | 0.001 | 0.0057 | 0.0005 | 0.003 | 0.0059 | 0.0083 | 0.0017 |
Punctate Nuclear | 0.0059 | 0.0001 | 0.0015 | 0.0032 | 0.0001 | 0.0003 | 0.001 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0001 | 0.0065 | 0.0059 | 0.0103 | 0.0009 | 0.0066 | 0.0057 |
Vacuole | 0.0014 | 0.0019 | 0.0017 | 0.0059 | 0.0005 | 0.0013 | 0.0015 | 0.0106 | 0.0008 | 0.0022 | 0.0007 | 0.0018 | 0.004 | 0.0009 | 0.0022 | 0.002 | 0.0342 | 0.0064 |
Vacuole Periphery | 0.0003 | 0.0001 | 0.0003 | 0.002 | 0.0001 | 0.0001 | 0.0004 | 0.0008 | 0.0001 | 0.0018 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.001 | 0.001 | 0.0053 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.369 | 19.2519 | 21.0084 | 32.9109 | 20.2574 | 24.9004 | 27.29 | 29.8824 | 32.4742 | 27.1872 |
Translational Efficiency | 1.217 | 1.1311 | 1.3338 | 0.9923 | 1.0216 | 1.1245 | 1.0489 | 1.0005 | 1.2309 | 1.0928 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1398 | 1668 | 337 | 1576 | 1017 | 1539 | 116 | 138 | 2415 | 3207 | 453 | 1714 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 881.66 | 1026.48 | 1869.58 | 1441.92 | 911.21 | 998.03 | 1466.11 | 1560.45 | 894.10 | 1012.83 | 1766.26 | 1451.46 |
Standard Deviation | 130.55 | 163.78 | 283.16 | 252.19 | 127.20 | 168.12 | 218.00 | 259.48 | 129.97 | 166.48 | 320.67 | 254.84 |
Intensity Change Log 2 | — | 0.219411 | 1.084420 | 0.709697 | — | 0.131300 | 0.686138 | 0.776107 | — | 0.175302 | 0.895714 | 0.743831 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.139067* | 0.182548* | 0.115296* | 0.213667* | 0.148436* | 0.156031* | 0.202759* | 0.249293* | 0.143013* | 0.169822* | 0.137693* | 0.216535* |
Bud Neck | 0.104658 | 0.172530 | 0.135575 | 0.068512 | 0.145518 | 0.174153 | 0.157536 | 0.065026 | 0.121865 | 0.173309 | 0.141199 | 0.068231 |
Bud Site | 0.089231 | 0.183747* | 0.049342 | 0.200416* | 0.169324* | 0.191053* | 0.201574* | 0.223781* | 0.122959* | 0.187253* | 0.088325 | 0.202297* |
Cell Periphery | 0.563067* | 0.373917* | 0.258750* | 0.374662* | 0.442896* | 0.398958* | 0.268432* | 0.213031* | 0.512461* | 0.385934* | 0.261229* | 0.361648* |
Cytoplasm | 0.002621 | 0.003966 | 0.000367 | 0.001242 | 0.005047 | 0.003553 | 0.007084 | 0.000528 | 0.003643 | 0.003768 | 0.002087 | 0.001184 |
Cytoplasmic Foci | 0.002366 | 0.004294 | 0.000279 | 0.001657 | 0.003795 | 0.004317 | 0.006169 | 0.001859 | 0.002968 | 0.004305 | 0.001787 | 0.001673 |
Eisosomes | 0.067128 | 0.059474 | 0.400258* | 0.101741* | 0.054894 | 0.049686 | 0.096706 | 0.194323* | 0.061976 | 0.054777 | 0.322528* | 0.109195* |
Endoplasmic Reticulum | 0.005810 | 0.002450 | 0.025537 | 0.012287 | 0.005772 | 0.002515 | 0.019943 | 0.032372 | 0.005794 | 0.002481 | 0.024105 | 0.013904 |
Endosome | 0.004534 | 0.002771 | 0.002385 | 0.004101 | 0.003609 | 0.003270 | 0.004803 | 0.004481 | 0.004144 | 0.003010 | 0.003004 | 0.004132 |
Golgi | 0.001841 | 0.002191 | 0.000761 | 0.003333 | 0.001516 | 0.001775 | 0.008637 | 0.003455 | 0.001704 | 0.001992 | 0.002778 | 0.003343 |
Lipid Particles | 0.000687 | 0.001423 | 0.001391 | 0.007342 | 0.001961 | 0.001384 | 0.012372 | 0.002416 | 0.001224 | 0.001404 | 0.004203 | 0.006945 |
Mitochondria | 0.010578 | 0.005474 | 0.002194 | 0.005208 | 0.005469 | 0.006025 | 0.000727 | 0.004400 | 0.008426 | 0.005738 | 0.001818 | 0.005143 |
Mitotic Spindle | 0.000514 | 0.000275 | 0.000638 | 0.001856 | 0.000399 | 0.000573 | 0.000371 | 0.000436 | 0.000466 | 0.000418 | 0.000570 | 0.001741 |
None | 0.000303 | 0.000141 | 0.000068 | 0.000085 | 0.000197 | 0.000150 | 0.000023 | 0.000055 | 0.000259 | 0.000145 | 0.000056 | 0.000082 |
Nuclear Periphery | 0.000087 | 0.000045 | 0.000478 | 0.000131 | 0.000055 | 0.000044 | 0.000094 | 0.000235 | 0.000074 | 0.000044 | 0.000379 | 0.000139 |
Nuclear Periphery Foci | 0.000556 | 0.000154 | 0.000162 | 0.000096 | 0.000196 | 0.000181 | 0.000598 | 0.000171 | 0.000405 | 0.000167 | 0.000274 | 0.000102 |
Nucleolus | 0.000022 | 0.000016 | 0.000019 | 0.000015 | 0.000050 | 0.000127 | 0.000009 | 0.000010 | 0.000034 | 0.000069 | 0.000016 | 0.000015 |
Nucleus | 0.000263 | 0.000200 | 0.000293 | 0.000206 | 0.000673 | 0.000960 | 0.000790 | 0.000232 | 0.000436 | 0.000565 | 0.000420 | 0.000208 |
Peroxisomes | 0.005901 | 0.003282 | 0.000080 | 0.001041 | 0.009080 | 0.003849 | 0.001718 | 0.000473 | 0.007240 | 0.003555 | 0.000499 | 0.000995 |
Vacuole | 0.000582 | 0.000933 | 0.006014 | 0.001874 | 0.000963 | 0.001026 | 0.009591 | 0.003235 | 0.000742 | 0.000978 | 0.006930 | 0.001983 |
Vacuole Periphery | 0.000186 | 0.000169 | 0.000112 | 0.000530 | 0.000150 | 0.000369 | 0.000064 | 0.000189 | 0.000171 | 0.000265 | 0.000100 | 0.000503 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.78 | 1.85 | -7.84 | -3.19 | -7.37 | -0.78 | -1.96 | -3.87 | -3.63 | -1.28 | -4.05 | 0.45 | -8.75 | -5.66 | -6.10 |
Bud Neck | -9.08 | -2.60 | 6.45 | 16.14 | 5.96 | -3.18 | -0.59 | 7.48 | 10.65 | 4.44 | -8.96 | -1.88 | 11.03 | 21.15 | 7.41 |
Bud Site | -12.06 | 5.32 | -13.51 | -1.84 | -17.01 | -2.13 | -1.29 | -2.06 | -1.26 | -0.64 | -10.24 | 3.76 | -10.17 | -1.92 | -10.96 |
Cell Periphery | 15.35 | 17.35 | 14.96 | -0.04 | -6.72 | 3.13 | 5.84 | 9.29 | 7.80 | 1.56 | 13.55 | 16.75 | 13.88 | 2.42 | -6.47 |
Cytoplasm | -1.73 | 5.48 | 3.47 | 4.06 | -4.44 | 1.59 | -0.65 | 6.11 | 5.33 | 2.07 | -0.23 | 1.67 | 6.27 | 5.82 | 1.10 |
Cytoplasmic Foci | -2.52 | 6.31 | 1.40 | 3.30 | -3.55 | -0.73 | -0.85 | 1.34 | 1.73 | 1.40 | -2.40 | 1.47 | 2.70 | 4.33 | 0.15 |
Eisosomes | 1.82 | -19.76 | -6.54 | -8.31 | 17.46 | 1.27 | -2.65 | -6.43 | -6.70 | -3.70 | 2.43 | -17.92 | -9.67 | -11.57 | 14.24 |
Endoplasmic Reticulum | 4.44 | -5.07 | -5.02 | -8.21 | 3.32 | 3.23 | -2.78 | -3.03 | -3.42 | -1.24 | 5.46 | -5.77 | -5.93 | -8.90 | 3.03 |
Endosome | 2.29 | 3.12 | 0.51 | -1.98 | -2.96 | 0.35 | -0.72 | -0.66 | -0.94 | 0.17 | 1.85 | 1.76 | 0.00 | -1.82 | -1.73 |
Golgi | -0.41 | 1.85 | -1.51 | -1.12 | -3.14 | -0.62 | -1.88 | -1.12 | -0.94 | 1.24 | -0.59 | -1.03 | -1.97 | -1.59 | -0.46 |
Lipid Particles | -1.17 | -1.60 | -4.90 | -3.98 | -4.20 | 0.80 | -1.99 | -0.41 | -1.05 | 1.89 | -0.40 | -2.12 | -4.49 | -4.26 | -1.48 |
Mitochondria | 3.15 | 5.17 | 3.19 | 0.26 | -2.98 | -0.46 | 6.12 | 0.67 | 0.96 | -2.59 | 2.49 | 6.36 | 2.79 | 0.64 | -3.81 |
Mitotic Spindle | 4.62 | -1.81 | -1.77 | -2.08 | -1.61 | -0.67 | 0.21 | -0.45 | 0.55 | -0.44 | 0.14 | -1.77 | -1.83 | -1.81 | -1.68 |
None | 3.12 | 4.63 | 4.27 | 4.08 | -2.11 | 1.54 | 7.15 | 5.71 | 5.41 | -5.18 | 3.44 | 6.47 | 5.55 | 5.29 | -3.62 |
Nuclear Periphery | 2.57 | -7.82 | -3.51 | -5.20 | 6.93 | 0.94 | -2.16 | -3.00 | -3.14 | -2.27 | 2.79 | -8.03 | -5.96 | -7.26 | 6.18 |
Nuclear Periphery Foci | 0.91 | 0.90 | 1.04 | 1.53 | 2.87 | 0.31 | -2.25 | 0.34 | 0.14 | 2.27 | 0.93 | 0.51 | 1.18 | 2.22 | 3.44 |
Nucleolus | 1.62 | 0.86 | 2.34 | 0.18 | 1.06 | -0.73 | 1.30 | 1.28 | 1.18 | -0.26 | -0.71 | 1.29 | 1.40 | 1.14 | 0.54 |
Nucleus | 3.16 | -0.82 | 2.94 | -0.53 | 2.59 | -0.58 | -0.22 | 1.25 | 1.79 | 1.47 | -0.58 | 0.09 | 1.51 | 1.81 | 2.10 |
Peroxisomes | 2.39 | 6.53 | 5.25 | 3.27 | -3.80 | 3.39 | 4.70 | 5.93 | 5.94 | 1.94 | 4.08 | 8.29 | 7.52 | 5.32 | -1.75 |
Vacuole | -1.11 | -6.75 | -2.56 | -1.68 | 4.41 | -0.12 | -2.23 | -2.03 | -1.99 | 1.60 | -0.93 | -5.30 | -2.58 | -2.03 | 3.97 |
Vacuole Periphery | 0.17 | 1.10 | -2.06 | -2.09 | -2.68 | -1.63 | 1.99 | -0.44 | 1.19 | -1.55 | -1.11 | 1.64 | -2.24 | -1.50 | -2.81 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|