Standard name
Human Ortholog
Description Negative regulator of WASP-Arp23 complex; involved in endocytic site formation; directly inhibits Las17p stimulation of Arp23 complex-mediated actin assembly in vitro; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.49 0.5 0.64 0.58 0.61 0.52 0.53 0.49 0.42 0.39 0.39 0.45 0.68 0.63 0.54 0.42 0.45 0.43 0.37 0.38 0.37 0.4 0.3 0.29
Bud 0.12 0.2 0.17 0.19 0.31 0.23 0.23 0.19 0.22 0.17 0.2 0.21 0.12 0.13 0.08 0.11 0.09 0.14 0.09 0.09 0.06 0 0 0
Bud Neck 0.06 0.06 0.05 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.18 0.28 0.21 0.1 0.07 0.06
Bud Site 0.23 0.23 0.15 0.18 0.17 0.21 0.11 0.06 0.08 0.08 0 0.09 0.13 0.05 0.05 0.07 0.07 0.09 – – – – – –
Cell Periphery 0.15 0.19 0.19 0.2 0.1 0.16 0.18 0.17 0.19 0.15 0.19 0.22 0.33 0.45 0.5 0.31 0.31 0.22 0.22 0.14 0.2 0.42 0.52 0.57
Cytoplasm 0.1 0.05 0.06 0.1 0.09 0.09 0.07 0.12 0 0.05 0.05 0.06 0 0 0.07 0.07 0.06 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.23 0.33 0.17 0 0 0 0 0 0
Mitochondria 0.4 0.3 0.15 0.27 0.16 0.33 0.45 0.28 0.66 0.72 0.75 0.57 0.26 0.23 0.22 0.15 0.13 0.12 0 0 0.05 0 0 0
Nucleus 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.28 0.22 0.08 0.12 0.07 0.21 0.17 0.15 0.15 0.1 0.11 0.13 0.45 0.5 0.44 0.1 0.11 0.1 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 93 76 163 115 142 126 174 120 102 106 88 128 174 173 175 93 99 87 81 78 109 75 94 77
Bud 23 30 43 38 72 55 76 47 54 47 45 59 32 35 25 24 20 29 19 19 16 1 8 3
Bud Neck 11 9 14 12 18 4 8 5 5 4 2 7 1 0 0 2 15 7 38 56 62 18 20 14
Bud Site 43 35 39 36 40 51 37 15 20 22 10 25 34 13 15 15 15 19 – – – – – –
Cell Periphery 28 29 48 39 23 38 59 42 46 41 42 63 85 122 162 68 68 44 48 28 57 80 162 149
Cytoplasm 19 7 16 19 20 21 22 29 7 13 11 16 9 7 23 16 14 17 0 0 3 0 1 0
Endoplasmic Reticulum 3 1 8 5 1 6 8 17 5 9 7 8 5 4 10 2 6 8 3 3 8 4 8 4
Endosome 1 1 1 1 0 0 1 0 2 0 0 0 0 2 1 0 0 2 4 2 2 1 2 0
Golgi 0 1 3 2 2 0 1 5 1 0 0 8 0 1 2 50 72 35 1 0 0 0 2 1
Mitochondria 76 46 39 54 36 80 146 70 159 195 167 165 67 62 72 32 28 25 8 7 16 3 5 6
Nucleus 6 4 24 6 8 3 11 11 3 7 2 9 1 1 0 1 6 6 1 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 2 1 0 0 0 0 0 1 0 1 0 2 0 3 0 0 0
Nucleolus 5 3 1 5 6 4 10 6 7 4 2 4 0 0 0 9 8 6 1 0 0 0 0 0
Peroxisomes 54 33 21 23 16 50 56 36 35 26 24 37 115 136 142 22 25 20 2 1 3 0 0 1
SpindlePole 0 2 13 7 18 7 7 11 1 4 1 5 0 1 0 9 8 22 0 0 0 0 0 0
Vac/Vac Membrane 0 2 6 1 2 0 6 8 6 7 3 7 0 0 0 4 3 5 3 4 5 1 1 0
Unique Cell Count 190 152 256 198 231 242 327 247 241 271 223 287 256 273 325 220 218 203 221 204 293 192 310 264
Labelled Cell Count 363 279 439 363 404 445 622 424 454 485 404 541 523 557 628 347 388 332 221 204 293 192 310 264


Bud Neck, Cell Periphery, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 8.3 8.6 7.6 8.4 7.5 7.9 8.1 7.1 6.8 6.7 7.6 9.0 9.6 10.4 14.9 14.4 12.0 7.9 7.8 8.4
Std Deviation (1e-4) 2.0 1.6 1.6 1.6 1.7 1.6 1.7 1.6 1.6 1.5 1.5 1.7 1.4 1.7 2.0 3.6 3.5 3.2 2.1 1.5 1.6
Intensity Change (Log2) – – – -0.18 -0.03 -0.2 -0.12 -0.09 -0.28 -0.34 -0.37 -0.17 0.07 0.16 0.27 0.79 0.75 0.48 -0.13 -0.14 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.2 -0.5 -2.6 -2.5 -3.4 -4.8 -5.6 -5.3 -4.4 1.0 -0.1 -2.4 -4.7 -4.0 -4.4
Bud 0.7 3.7 1.7 1.9 0.7 1.6 0.2 1.0 1.1 -1.4 -1.3 -3.4 -1.8 -2.4 -0.7
Bud Neck 0.3 1.0 -2.3 -1.9 -2.0 -2.0 -2.5 -2.8 -1.8 -3.4 -3.9 -4.3 -2.8 0.6 -1.0
Bud Site 0.8 0.6 1.7 -1.4 -3.3 -2.4 -2.6 -3.9 -2.4 -0.6 -4.0 -4.4 -2.9 -2.9 -1.9
Cell Periphery 0.3 -2.7 -0.9 -0.2 -0.5 0.1 -1.1 0 0.9 3.7 6.4 7.7 3.1 3.1 0.8
Cytoplasm 1.3 1.0 1.0 0.2 2.2 -1.8 -0.7 -0.6 -0.3 -1.4 -2.1 0.4 0.4 0.1 0.9
Endoplasmic Reticulum -0.4 0 -0.4 -0.5 1.9 -0.7 0.1 0 -0.2 -0.8 -1.3 0 0 -0.2 0.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 1.3 0 0 0 7.5 9.5 6.2
Mitochondria 3.2 0.1 4.7 7.6 3.6 11.5 13.1 13.2 10.1 3.1 2.2 2.1 -0.2 -0.7 -0.9
Nucleus -2.7 -2.6 -4.0 -3.0 -2.2 -4.0 -3.3 -4.1 -3.0 -4.7 -4.9 -5.6 -4.3 -3.0 -2.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1.2 -0.5 4.0 3.2 2.3 2.2 0.6 1.0 1.8 9.4 10.5 9.5 0.7 1.2 0.6
SpindlePole -0.8 1.2 -1.2 -1.9 -0.3 -3.1 -2.3 -3.0 -2.2 -3.7 -3.4 -4.1 -0.5 -0.7 2.3
Vacuole 0 0 0 -0.4 0.6 0.1 0.2 0 0.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.9403 7.8561 7.0583 5.697 5.5518 7.4041 5.1515 5.745 4.6819 4.4534 4.5507 5.2484 5.5879 6.8977 5.8104 5.2198 4.2837 6.1125
Actin 0.2804 0.1295 0.1713 0.1521 0.4462 0.2561 0.2088 0.1379 0.1358 0.1497 0.2952 0.2116 0.1235 0.0582 0.0657 0.0496 0.0549 0.0882
Bud 0.0035 0.0024 0.0029 0.0041 0.0031 0.0031 0.0037 0.0021 0.0035 0.0024 0.008 0.0041 0.0039 0.0018 0.0026 0.0013 0.006 0.0035
Bud Neck 0.1107 0.043 0.4928 0.4462 0.2547 0.4867 0.0757 0.027 0.4623 0.4083 0.1973 0.4205 0.1031 0.0646 0.496 0.502 0.088 0.374
Bud Periphery 0.0082 0.003 0.0151 0.0403 0.0298 0.0272 0.0107 0.0041 0.0083 0.0218 0.0754 0.0307 0.0121 0.0037 0.0078 0.0088 0.0888 0.0412
Bud Site 0.3422 0.7516 0.173 0.0149 0.0086 0.0185 0.2555 0.7375 0.2431 0.0624 0.0436 0.0163 0.3067 0.7531 0.2412 0.0126 0.0179 0.0236
Cell Periphery 0.0685 0.0089 0.038 0.1164 0.0865 0.0973 0.1271 0.0397 0.0397 0.1583 0.1447 0.1747 0.1484 0.0477 0.0456 0.0763 0.2876 0.198
Cytoplasm 0.0049 0.0175 0.0107 0.0023 0.0015 0.0073 0.0056 0.0021 0.0035 0.0023 0.0026 0.0038 0.0077 0.0018 0.0037 0.0035 0.0071 0.0068
Cytoplasmic Foci 0.013 0.0033 0.0162 0.0188 0.0013 0.0019 0.0162 0.0014 0.017 0.0028 0.0228 0.0013 0.0252 0.0012 0.0139 0.0036 0.0381 0.0262
Eisosomes 0.1019 0.0346 0.0163 0.0313 0.1601 0.0887 0.2111 0.0282 0.0321 0.0959 0.1613 0.1229 0.1164 0.0358 0.0224 0.1687 0.0793 0.1207
Endoplasmic Reticulum 0.0025 0.0001 0.0043 0.0026 0.0004 0.0027 0.0014 0.0002 0.0003 0.0024 0.0014 0.001 0.0048 0.0007 0.0016 0.0264 0.0065 0.0045
Endosome 0.0057 0.002 0.0048 0.0343 0.0003 0.001 0.0171 0.0021 0.0099 0.0208 0.0087 0.0014 0.0147 0.0029 0.0137 0.0089 0.0557 0.0144
Golgi 0.0089 0.0006 0.0026 0.0185 0.0036 0.0017 0.0193 0.0041 0.0154 0.0172 0.0023 0.0036 0.0165 0.0009 0.0062 0.0154 0.0115 0.0042
Lipid Particles 0.0143 0.0003 0.0139 0.0234 0.0006 0.0011 0.0164 0.0005 0.0054 0.004 0.0153 0.0007 0.0141 0.0012 0.0136 0.01 0.0786 0.0091
Mitochondria 0.0055 0.0001 0.0111 0.0136 0.001 0.0005 0.0133 0.0006 0.0078 0.0419 0.0007 0.003 0.0313 0.0005 0.014 0.0239 0.0479 0.0104
None 0.0026 0.0002 0.0042 0.0006 0.0002 0.0024 0.0009 0.0002 0.0006 0.0007 0.0005 0.0004 0.0053 0.0044 0.005 0.0639 0.0163 0.0022
Nuclear Periphery 0.0013 0 0.0045 0.0041 0.0001 0.0004 0.0007 0.0002 0.0001 0.0023 0.0003 0.0002 0.0024 0.0005 0.0015 0.0118 0.0052 0.0008
Nucleolus 0.0031 0.0001 0.0014 0.0017 0.0001 0.0005 0.0007 0.0001 0.0006 0.0003 0.0002 0.0003 0.0063 0.0027 0.0031 0.0009 0.0057 0.0089
Nucleus 0.0017 0.0002 0.0048 0.0024 0.0001 0.0004 0.0004 0.0002 0.0004 0.0006 0.0002 0.0004 0.0405 0.0108 0.0261 0.0027 0.0507 0.0487
Peroxisomes 0.0134 0.0005 0.0085 0.0612 0.001 0.0008 0.0125 0.0004 0.0128 0.002 0.0183 0.001 0.0057 0.0005 0.003 0.0059 0.0083 0.0017
Punctate Nuclear 0.0059 0.0001 0.0015 0.0032 0.0001 0.0003 0.001 0.0001 0.0004 0.0002 0.0005 0.0001 0.0065 0.0059 0.0103 0.0009 0.0066 0.0057
Vacuole 0.0014 0.0019 0.0017 0.0059 0.0005 0.0013 0.0015 0.0106 0.0008 0.0022 0.0007 0.0018 0.004 0.0009 0.0022 0.002 0.0342 0.0064
Vacuole Periphery 0.0003 0.0001 0.0003 0.002 0.0001 0.0001 0.0004 0.0008 0.0001 0.0018 0.0001 0.0001 0.001 0.0001 0.001 0.001 0.0053 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.369 19.2519 21.0084 32.9109 20.2574 24.9004 27.29 29.8824 32.4742 27.1872
Translational Efficiency 1.217 1.1311 1.3338 0.9923 1.0216 1.1245 1.0489 1.0005 1.2309 1.0928

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1398 1668 337 1576 1017 1539 116 138 2415 3207 453 1714

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 881.66 1026.48 1869.58 1441.92 911.21 998.03 1466.11 1560.45 894.10 1012.83 1766.26 1451.46
Standard Deviation 130.55 163.78 283.16 252.19 127.20 168.12 218.00 259.48 129.97 166.48 320.67 254.84
Intensity Change Log 2 — 0.219411 1.084420 0.709697 — 0.131300 0.686138 0.776107 — 0.175302 0.895714 0.743831

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.139067* 0.182548* 0.115296* 0.213667* 0.148436* 0.156031* 0.202759* 0.249293* 0.143013* 0.169822* 0.137693* 0.216535*
Bud Neck 0.104658 0.172530 0.135575 0.068512 0.145518 0.174153 0.157536 0.065026 0.121865 0.173309 0.141199 0.068231
Bud Site 0.089231 0.183747* 0.049342 0.200416* 0.169324* 0.191053* 0.201574* 0.223781* 0.122959* 0.187253* 0.088325 0.202297*
Cell Periphery 0.563067* 0.373917* 0.258750* 0.374662* 0.442896* 0.398958* 0.268432* 0.213031* 0.512461* 0.385934* 0.261229* 0.361648*
Cytoplasm 0.002621 0.003966 0.000367 0.001242 0.005047 0.003553 0.007084 0.000528 0.003643 0.003768 0.002087 0.001184
Cytoplasmic Foci 0.002366 0.004294 0.000279 0.001657 0.003795 0.004317 0.006169 0.001859 0.002968 0.004305 0.001787 0.001673
Eisosomes 0.067128 0.059474 0.400258* 0.101741* 0.054894 0.049686 0.096706 0.194323* 0.061976 0.054777 0.322528* 0.109195*
Endoplasmic Reticulum 0.005810 0.002450 0.025537 0.012287 0.005772 0.002515 0.019943 0.032372 0.005794 0.002481 0.024105 0.013904
Endosome 0.004534 0.002771 0.002385 0.004101 0.003609 0.003270 0.004803 0.004481 0.004144 0.003010 0.003004 0.004132
Golgi 0.001841 0.002191 0.000761 0.003333 0.001516 0.001775 0.008637 0.003455 0.001704 0.001992 0.002778 0.003343
Lipid Particles 0.000687 0.001423 0.001391 0.007342 0.001961 0.001384 0.012372 0.002416 0.001224 0.001404 0.004203 0.006945
Mitochondria 0.010578 0.005474 0.002194 0.005208 0.005469 0.006025 0.000727 0.004400 0.008426 0.005738 0.001818 0.005143
Mitotic Spindle 0.000514 0.000275 0.000638 0.001856 0.000399 0.000573 0.000371 0.000436 0.000466 0.000418 0.000570 0.001741
None 0.000303 0.000141 0.000068 0.000085 0.000197 0.000150 0.000023 0.000055 0.000259 0.000145 0.000056 0.000082
Nuclear Periphery 0.000087 0.000045 0.000478 0.000131 0.000055 0.000044 0.000094 0.000235 0.000074 0.000044 0.000379 0.000139
Nuclear Periphery Foci 0.000556 0.000154 0.000162 0.000096 0.000196 0.000181 0.000598 0.000171 0.000405 0.000167 0.000274 0.000102
Nucleolus 0.000022 0.000016 0.000019 0.000015 0.000050 0.000127 0.000009 0.000010 0.000034 0.000069 0.000016 0.000015
Nucleus 0.000263 0.000200 0.000293 0.000206 0.000673 0.000960 0.000790 0.000232 0.000436 0.000565 0.000420 0.000208
Peroxisomes 0.005901 0.003282 0.000080 0.001041 0.009080 0.003849 0.001718 0.000473 0.007240 0.003555 0.000499 0.000995
Vacuole 0.000582 0.000933 0.006014 0.001874 0.000963 0.001026 0.009591 0.003235 0.000742 0.000978 0.006930 0.001983
Vacuole Periphery 0.000186 0.000169 0.000112 0.000530 0.000150 0.000369 0.000064 0.000189 0.000171 0.000265 0.000100 0.000503

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.78 1.85 -7.84 -3.19 -7.37 -0.78 -1.96 -3.87 -3.63 -1.28 -4.05 0.45 -8.75 -5.66 -6.10
Bud Neck -9.08 -2.60 6.45 16.14 5.96 -3.18 -0.59 7.48 10.65 4.44 -8.96 -1.88 11.03 21.15 7.41
Bud Site -12.06 5.32 -13.51 -1.84 -17.01 -2.13 -1.29 -2.06 -1.26 -0.64 -10.24 3.76 -10.17 -1.92 -10.96
Cell Periphery 15.35 17.35 14.96 -0.04 -6.72 3.13 5.84 9.29 7.80 1.56 13.55 16.75 13.88 2.42 -6.47
Cytoplasm -1.73 5.48 3.47 4.06 -4.44 1.59 -0.65 6.11 5.33 2.07 -0.23 1.67 6.27 5.82 1.10
Cytoplasmic Foci -2.52 6.31 1.40 3.30 -3.55 -0.73 -0.85 1.34 1.73 1.40 -2.40 1.47 2.70 4.33 0.15
Eisosomes 1.82 -19.76 -6.54 -8.31 17.46 1.27 -2.65 -6.43 -6.70 -3.70 2.43 -17.92 -9.67 -11.57 14.24
Endoplasmic Reticulum 4.44 -5.07 -5.02 -8.21 3.32 3.23 -2.78 -3.03 -3.42 -1.24 5.46 -5.77 -5.93 -8.90 3.03
Endosome 2.29 3.12 0.51 -1.98 -2.96 0.35 -0.72 -0.66 -0.94 0.17 1.85 1.76 0.00 -1.82 -1.73
Golgi -0.41 1.85 -1.51 -1.12 -3.14 -0.62 -1.88 -1.12 -0.94 1.24 -0.59 -1.03 -1.97 -1.59 -0.46
Lipid Particles -1.17 -1.60 -4.90 -3.98 -4.20 0.80 -1.99 -0.41 -1.05 1.89 -0.40 -2.12 -4.49 -4.26 -1.48
Mitochondria 3.15 5.17 3.19 0.26 -2.98 -0.46 6.12 0.67 0.96 -2.59 2.49 6.36 2.79 0.64 -3.81
Mitotic Spindle 4.62 -1.81 -1.77 -2.08 -1.61 -0.67 0.21 -0.45 0.55 -0.44 0.14 -1.77 -1.83 -1.81 -1.68
None 3.12 4.63 4.27 4.08 -2.11 1.54 7.15 5.71 5.41 -5.18 3.44 6.47 5.55 5.29 -3.62
Nuclear Periphery 2.57 -7.82 -3.51 -5.20 6.93 0.94 -2.16 -3.00 -3.14 -2.27 2.79 -8.03 -5.96 -7.26 6.18
Nuclear Periphery Foci 0.91 0.90 1.04 1.53 2.87 0.31 -2.25 0.34 0.14 2.27 0.93 0.51 1.18 2.22 3.44
Nucleolus 1.62 0.86 2.34 0.18 1.06 -0.73 1.30 1.28 1.18 -0.26 -0.71 1.29 1.40 1.14 0.54
Nucleus 3.16 -0.82 2.94 -0.53 2.59 -0.58 -0.22 1.25 1.79 1.47 -0.58 0.09 1.51 1.81 2.10
Peroxisomes 2.39 6.53 5.25 3.27 -3.80 3.39 4.70 5.93 5.94 1.94 4.08 8.29 7.52 5.32 -1.75
Vacuole -1.11 -6.75 -2.56 -1.68 4.41 -0.12 -2.23 -2.03 -1.99 1.60 -0.93 -5.30 -2.58 -2.03 3.97
Vacuole Periphery 0.17 1.10 -2.06 -2.09 -2.68 -1.63 1.99 -0.44 1.19 -1.55 -1.11 1.64 -2.24 -1.50 -2.81
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Negative regulator of WASP-Arp23 complex; involved in endocytic site formation; directly inhibits Las17p stimulation of Arp23 complex-mediated actin assembly in vitro; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization
Localization
Cell Percentages cell periphery (36%), actin (20%), mixed (37%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Syp1

Syp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Syp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available