Standard name
Human Ortholog
Description Subunit of the Set3C deacetylase complex; interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0.07 0.06 0 0 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0.05 0.05 0.05 0 0.05 0.05 0.06 0.05 0.06 0.06 0.07 0.15 0.2 0.14 0.22 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.55 0.61 0.71 0.57 0.72 0.74 0.7 0.79 0.72 0.7 0.72 0.12 0.12 0.29 0.14 0.06 0.14 0 0 0 0 0 0
Nucleus 0.88 0.8 0.78 0.81 0.77 0.73 0.74 0.73 0.66 0.68 0.61 0.63 0.81 0.89 0.76 0.67 0.77 0.65 0.57 0.68 0.66 0.73 0.63 0.53
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.1 0 0 0 0 0.07 0.12 0.1 0.14 0.14 0.2 0.23 0.16 0.12 0.05 0 0 0.28 0.12 0.16 0.17 0.16 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0 0 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0
Bud 0 0 1 1 3 4 5 7 8 7 2 5 0 0 0 0 0 0 1 1 2 0 3 3
Bud Neck 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0
Bud Site 1 0 0 3 9 10 17 32 23 16 7 22 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 14 2 7 9 12 16 11 21 20 24 10 18 3 7 17 34 22 28 3 0 0 0 1 2
Endoplasmic Reticulum 0 0 2 1 0 0 0 0 1 0 1 0 0 0 0 8 4 5 1 0 2 0 1 0
Endosome 3 0 0 0 1 0 0 0 0 0 0 0 0 0 0 7 3 1 4 0 3 0 3 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 5 0 0 0 0 1 5
Mitochondria 4 59 155 140 140 248 332 327 303 302 140 221 6 12 32 24 10 18 7 3 5 0 3 4
Nucleus 270 86 199 159 191 251 335 338 255 284 123 194 39 87 84 112 122 84 191 86 172 42 106 97
Nuclear Periphery 2 0 1 2 2 0 4 3 4 8 5 4 0 0 0 1 0 0 2 1 0 0 0 1
Nucleolus 50 11 3 5 4 10 32 55 37 58 27 62 11 16 13 9 3 3 94 15 41 9 27 31
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 6 0 9 5 6 2 10 0 1 2 5 2 0 0 0 2 4 5 7 2 8 2 5 6
Vac/Vac Membrane 2 0 0 0 0 2 1 1 1 1 1 1 2 1 1 6 4 5 14 10 18 0 7 17
Unique Cell Count 306 107 254 197 247 346 451 464 385 418 200 307 48 98 110 168 158 129 337 127 261 59 168 184
Labelled Cell Count 352 158 377 325 368 543 747 785 653 703 321 529 61 123 147 204 175 154 337 127 261 59 168 184


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 3.9 3.6 3.6 3.8 3.6 3.2 3.4 3.2 3.3 3.4 3.2 5.3 5.0 4.4 7.4 7.4 8.6 4.7 5.6 6.3
Std Deviation (1e-4) 0.9 0.6 0.7 1.3 1.2 1.2 0.8 1.0 1.3 1.3 1.5 1.4 1.2 0.9 0.9 2.2 2.5 3.2 0.7 1.9 2.2
Intensity Change (Log2) – – – -0.0 0.07 -0.03 -0.16 -0.09 -0.17 -0.14 -0.09 -0.18 0.55 0.46 0.27 1.03 1.03 1.25 0.37 0.64 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6104 1.8795 1.7381 1.7272 0.9734 1.9018 2.8661 2.5921 2.6401 2.1154 2.3141 2.5375 3.4787 3.5894 3.5614 3.9472 3.574 3.2423
Actin 0.0073 0 0.0002 0 0.0001 0 0.0138 0.0004 0.0069 0.0331 0.0063 0.0051 0.0021 0 0 0.0088 0.001 0.0005
Bud 0.0001 0.0001 0 0 0.0002 0.0001 0.0005 0.0003 0.0002 0.0001 0.0009 0.0002 0.0001 0 0 0.0002 0.0001 0
Bud Neck 0.0003 0.0004 0.0001 0.0001 0.0069 0.0023 0.0014 0.0004 0.0004 0.0005 0.0013 0.0011 0.0003 0.0001 0.0001 0.0012 0.0022 0.001
Bud Periphery 0.0001 0.0001 0.0001 0 0.0004 0.0001 0.0006 0.0008 0.0005 0.0001 0.0034 0.0006 0.0003 0 0 0.0002 0.0003 0.0001
Bud Site 0.0004 0.0011 0.0001 0.0001 0.0008 0.0002 0.0047 0.0006 0.0008 0.0007 0.0017 0.0003 0.0004 0.0001 0.0001 0.0013 0.0005 0.0001
Cell Periphery 0 0 0 0 0.0002 0 0.0001 0.0002 0.0001 0 0.0002 0.0001 0.0001 0 0 0 0.0001 0
Cytoplasm 0.0013 0.0004 0.0002 0.0002 0.0001 0.0001 0.0108 0.0001 0.0023 0.0001 0.0015 0.0001 0.0001 0.0001 0 0.0011 0.0017 0
Cytoplasmic Foci 0.0046 0.0001 0 0 0 0 0.0202 0.0001 0.0122 0.0027 0.0042 0.0028 0.0025 0 0 0.0067 0.0126 0.0002
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0.0001 0.0001 0.0001 0.0002 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0007 0 0.0001 0 0 0 0.0076 0.0001 0.0003 0 0.0015 0.0011 0.0001 0 0 0.0002 0.0006 0
Endosome 0.0067 0.0001 0.0006 0 0.0002 0.0001 0.0427 0.0003 0.0044 0.0005 0.0166 0.0068 0.0064 0 0 0.0056 0.0151 0.0013
Golgi 0.0016 0 0.0001 0 0 0 0.0171 0.0001 0.0062 0.0017 0.0163 0.0045 0.0025 0 0 0.0048 0.0008 0.0027
Lipid Particles 0.0045 0 0.0002 0 0 0 0.0054 0.0003 0.009 0.0017 0.0068 0.0102 0.0138 0 0 0.0069 0.0072 0.0002
Mitochondria 0.0007 0.0002 0.0074 0.0001 0.0006 0.0001 0.0042 0.0035 0.0046 0.0004 0.054 0.012 0.0195 0.0001 0.0002 0.0016 0.0021 0.0067
None 0.0039 0.0002 0.0008 0.0001 0.0001 0.0001 0.0058 0.0001 0.0002 0.0001 0.0007 0.0001 0.0002 0 0 0.0146 0.0037 0
Nuclear Periphery 0.003 0.0033 0.0026 0.0014 0.0005 0.0011 0.0174 0.0005 0.0016 0.0003 0.0306 0.0062 0.0015 0.0007 0.0005 0.0034 0.0164 0.0001
Nucleolus 0.0209 0.0554 0.0288 0.0215 0.2943 0.1187 0.0432 0.0392 0.0371 0.0191 0.1749 0.0915 0.0415 0.039 0.0351 0.0558 0.1839 0.1008
Nucleus 0.9319 0.9214 0.953 0.9758 0.6912 0.853 0.7737 0.9486 0.8948 0.9333 0.6587 0.8461 0.9015 0.958 0.9625 0.8696 0.6945 0.8786
Peroxisomes 0.0014 0 0.0001 0 0 0 0.016 0.0001 0.0136 0.0039 0.0048 0.0063 0.0035 0 0 0.007 0.0011 0.0001
Punctate Nuclear 0.01 0.0167 0.0047 0.0006 0.0033 0.0239 0.011 0.0029 0.0029 0.0015 0.0077 0.0034 0.0027 0.0017 0.0015 0.0104 0.0546 0.0072
Vacuole 0.0002 0.0002 0.0002 0.0001 0.0007 0.0001 0.0018 0.0009 0.0011 0 0.0046 0.0009 0.0004 0 0 0.0002 0.0008 0.0001
Vacuole Periphery 0.0002 0.0001 0.0005 0 0.0002 0 0.0019 0.0004 0.0007 0 0.003 0.0007 0.0004 0 0 0.0001 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.8355 17.6856 16.1061 20.1036 20.7927 14.0808 24.4522 26.6069 23.434 25.1957
Translational Efficiency 0.6073 0.703 0.6375 0.4256 0.4549 0.7373 0.4495 0.4105 0.4492 0.4715

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1787 63 394 1100 1642 1689 444 1308 3429 1752 838 2408

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 639.92 318.55 1037.08 866.65 683.92 771.02 968.95 877.99 660.99 754.75 1000.98 872.81
Standard Deviation 223.70 11.73 128.33 134.20 97.80 100.00 102.02 116.91 176.47 129.40 120.06 125.23
Intensity Change Log 2 — -1.006372 0.696564 0.437558 — 0.172941 0.502595 0.360377 — -0.280970 0.599614 0.398201

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000102 0.000045 0.001586 0.001191 0.000263 0.000563 0.001211 0.001401 0.000179 0.000544 0.001387 0.001305
Bud Neck 0.003502 0.000029 0.020837 0.029946 0.004760 0.007395 0.009140 0.033989 0.004104 0.007131 0.014639 0.032142
Bud Site 0.001625 0.000035 0.003012 0.017809 0.000663 0.002167 0.001242 0.009320 0.001165 0.002090 0.002074 0.013198
Cell Periphery 0.000139 0.000085 0.000058 0.000470 0.000100 0.000081 0.000052 0.000156 0.000120 0.000081 0.000055 0.000299
Cytoplasm 0.139664* 0.000058 0.000975 0.063247 0.029050 0.007634 0.001568 0.011780 0.086696 0.007362 0.001289 0.035291
Cytoplasmic Foci 0.012652 0.000046 0.000064 0.006751 0.004287 0.004245 0.000204 0.000702 0.008646 0.004094 0.000138 0.003466
Eisosomes 0.000026 0.000111 0.000047 0.000085 0.000027 0.000041 0.000053 0.000046 0.000026 0.000044 0.000050 0.000064
Endoplasmic Reticulum 0.000845 0.000001 0.005352 0.004432 0.001691 0.003849 0.005428 0.006373 0.001250 0.003711 0.005392 0.005486
Endosome 0.001574 0.000008 0.000239 0.006586 0.000635 0.002086 0.000277 0.002160 0.001124 0.002011 0.000259 0.004182
Golgi 0.000411 0.000006 0.000256 0.005563 0.000310 0.001552 0.000159 0.000606 0.000363 0.001496 0.000205 0.002870
Lipid Particles 0.001518 0.000037 0.000189 0.001980 0.001178 0.002447 0.000319 0.000334 0.001355 0.002361 0.000258 0.001086
Mitochondria 0.002901 0.000011 0.001689 0.013377 0.002556 0.011968 0.001107 0.005524 0.002736 0.011538 0.001381 0.009112
Mitotic Spindle 0.000740 0.000360 0.002574 0.038898 0.001931 0.008400 0.001806 0.031639 0.001311 0.008111 0.002167 0.034955
None 0.052681 0.998653* 0.001977 0.014801 0.024361 0.008182 0.003163 0.009571 0.039120 0.043798 0.002605 0.011960
Nuclear Periphery 0.000863 0.000000 0.000312 0.001698 0.000737 0.001877 0.000306 0.002449 0.000802 0.001809 0.000309 0.002106
Nuclear Periphery Foci 0.001153 0.000334 0.000386 0.000865 0.001095 0.001878 0.000484 0.001030 0.001125 0.001823 0.000438 0.000955
Nucleolus 0.029082 0.000059 0.065976 0.018221 0.054147 0.081610 0.052975 0.040449 0.041084 0.078678 0.059088 0.030295
Nucleus 0.747109* 0.000013 0.891289* 0.756554* 0.870286* 0.847523* 0.917093* 0.829845* 0.806093* 0.817047* 0.904960* 0.796365*
Peroxisomes 0.000724 0.000105 0.000998 0.002653 0.000997 0.002120 0.000614 0.000701 0.000855 0.002048 0.000795 0.001592
Vacuole 0.001993 0.000004 0.001928 0.010644 0.000703 0.003128 0.002636 0.008543 0.001375 0.003016 0.002303 0.009503
Vacuole Periphery 0.000697 0.000000 0.000256 0.004228 0.000224 0.001253 0.000162 0.003382 0.000470 0.001208 0.000206 0.003769

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.92 -11.83 -14.67 -15.38 1.27 -4.72 -11.62 -20.05 -12.75 -0.81 -6.22 -16.67 -24.41 -13.60 0.42
Bud Neck 7.53 -11.85 -15.67 -17.65 -4.40 -3.46 -6.94 -19.87 -17.98 -13.53 -4.68 -13.49 -25.09 -21.66 -11.77
Bud Site 3.53 -3.22 -6.80 -7.89 -5.64 -2.51 -5.29 -6.84 -4.81 -4.89 -1.61 -5.22 -9.39 -7.50 -7.42
Cell Periphery 2.94 5.60 -4.64 -5.95 -6.95 1.77 2.89 -2.15 -4.26 -5.41 4.08 6.18 -4.93 -7.29 -8.38
Cytoplasm 28.72 28.50 17.36 -11.67 -11.36 8.81 10.81 7.84 -1.06 -4.37 25.95 28.72 19.25 -7.86 -11.91
Cytoplasmic Foci 12.73 12.70 8.73 -5.69 -5.64 0.44 5.75 5.61 5.58 -0.94 5.29 13.61 10.74 3.55 -5.68
Eisosomes -2.16 -6.06 -9.31 0.92 -4.68 -5.94 -8.49 -11.75 -4.51 2.91 -6.51 -10.33 -13.72 -5.63 -1.23
Endoplasmic Reticulum 17.12 -10.08 -17.47 -20.12 -2.19 -8.01 -11.01 -17.16 -8.15 0.17 -9.66 -15.02 -24.73 -9.94 -1.35
Endosome 3.17 2.52 -2.43 -5.62 -5.10 -2.85 1.32 -2.38 1.34 -3.28 -1.51 2.72 -2.84 -0.80 -6.00
Golgi 5.62 1.34 -2.11 -2.57 -2.23 -3.17 1.81 -1.17 2.82 -2.39 -2.97 2.15 -2.21 0.10 -2.46
Lipid Particles 14.07 9.17 1.52 -4.25 -3.08 -2.52 4.51 5.62 4.64 1.57 -2.14 9.39 4.62 3.67 -2.41
Mitochondria 4.50 1.18 -3.14 -5.29 -3.89 -5.92 2.37 -2.28 5.04 -6.40 -5.90 2.45 -3.78 3.44 -5.73
Mitotic Spindle 1.83 -2.87 -9.45 -9.57 -5.95 -3.80 -2.07 -8.61 -6.34 -6.15 -4.50 -3.57 -12.82 -9.09 -8.52
None -342.99* 19.58 15.37 755.44* -6.96 9.06 12.41 9.49 1.57 -3.76 -0.76 22.85 18.08 7.96 -7.55
Nuclear Periphery 3.74 1.33 -3.77 -7.94 -5.84 -3.33 1.74 -4.17 -0.34 -4.81 -2.96 1.86 -5.45 -0.79 -7.41
Nuclear Periphery Foci 2.48 2.46 2.18 -1.26 -0.82 -1.83 0.12 1.25 3.12 1.05 -1.51 1.76 2.46 3.11 0.22
Nucleolus 17.23* -7.15 5.77 -13.36 9.79 -7.03 -0.51 4.30 10.71 3.12 -10.66 -5.62 6.08 14.73 8.87
Nucleus 105.10* -12.32 -4.92 -95.58* 6.62 2.92 -3.30 4.43 1.70 6.79 -1.94 -11.87 -1.97 0.22 9.39
Peroxisomes 7.41 -1.74 -1.58 -3.80 -0.85 -3.71 2.01 2.77 6.45 1.50 -4.66 0.58 0.04 4.24 -0.36
Vacuole 5.24 -1.22 -7.72 -9.39 -6.73 -3.71 -3.22 -6.54 -4.62 -2.41 -2.44 -3.33 -9.88 -7.54 -5.78
Vacuole Periphery 1.83 1.01 -2.21 -3.87 -3.32 -5.79 0.80 -2.68 -0.74 -2.72 -2.84 1.08 -3.29 -1.65 -4.24
Standard name
Human Ortholog
Description Subunit of the Set3C deacetylase complex; interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Snt1

Snt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available