Standard name
Human Ortholog
Description TFIID subunit (150 kDa); involved in RNA polymerase II transcription initiation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0.08 0 0 0.06 0.07 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0.07 0.13 0.16 0.08 0.08 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.41 0.5 0.73 0.75 0.44 0.74 0.65 0.69 0.8 0.79 0.84 0.83 0 0.06 0.22 0.13 0.07 0.17 0 0 0 0 0 0
Nucleus 0.88 0.79 0.73 0.78 0.84 0.73 0.69 0.68 0.66 0.68 0.67 0.68 0.91 0.84 0.79 0.81 0.81 0.72 0.78 0.79 0.64 0.79 0.77 0.67
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.15 0.08 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0.12 0.07 0.12 0.09 0.1 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.13 0 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Bud 0 0 0 2 8 6 13 19 9 8 6 21 0 0 2 1 0 1 0 1 0 0 3 8
Bud Neck 0 0 0 0 1 1 1 1 2 0 1 1 0 0 0 0 0 1 0 0 1 0 1 1
Bud Site 2 0 1 4 15 19 51 31 15 43 29 54 0 1 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 2 7 7 8 12 18 31 32 22 25 12 18 23 78 111 18 14 15 0 1 2 0 2 1
Endoplasmic Reticulum 1 0 0 1 0 0 0 0 0 0 0 1 0 0 3 3 3 5 0 0 0 0 0 2
Endosome 0 0 0 0 2 0 1 1 0 0 0 0 0 0 0 10 10 10 1 1 2 2 4 11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 2 2 2
Mitochondria 35 56 125 184 220 306 438 527 425 542 365 612 13 38 152 29 12 32 1 1 2 0 2 5
Nucleus 75 89 125 193 422 302 460 522 349 471 290 501 283 495 557 177 140 136 77 88 109 113 355 516
Nuclear Periphery 1 2 0 0 3 0 8 1 2 1 2 1 0 2 2 0 0 1 0 0 0 0 0 4
Nucleolus 13 9 2 2 17 5 16 25 23 27 16 51 6 6 17 5 1 5 12 7 20 12 44 136
Peroxisomes 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
SpindlePole 1 0 12 7 16 5 22 28 17 24 5 9 6 7 0 6 7 7 1 1 5 2 10 18
Vac/Vac Membrane 0 4 0 0 7 3 4 8 3 0 2 3 1 2 5 8 7 10 2 6 21 5 31 49
Unique Cell Count 85 112 171 246 503 414 671 767 528 688 436 737 310 586 704 219 173 188 99 112 171 143 462 773
Labelled Cell Count 130 167 272 401 724 665 1045 1196 867 1141 728 1272 332 629 852 260 195 224 99 112 171 143 462 773


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 4.0 3.4 3.5 3.8 3.5 3.3 3.3 3.0 3.0 3.1 2.7 5.3 5.0 4.4 5.7 6.0 6.7 5.1 5.0 5.2
Std Deviation (1e-4) 0.6 0.8 0.6 0.8 0.9 1.1 1.1 0.9 1.0 1.2 1.1 0.7 1.1 1.2 0.9 1.0 0.8 1.4 1.1 1.1 1.1
Intensity Change (Log2) – – – 0.04 0.13 0.03 -0.07 -0.08 -0.2 -0.18 -0.16 -0.32 0.64 0.55 0.35 0.74 0.81 0.97 0.57 0.54 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5262 1.0424 0.5591 0.7243 0.4544 0.9166 1.2095 1.8813 1.855 1.4166 1.4142 1.5992 0.6007 -0.2408 -0.096 0.0581 -0.6175 -0.1573
Actin 0.0069 0 0.0097 0.0107 0.0863 0.0004 0.0064 0.0009 0.0055 0.0595 0.0196 0.0006 0.0005 0 0.0001 0.0003 0.0001 0
Bud 0.0002 0 0.0003 0.0001 0.0008 0 0.0007 0 0.0024 0.0005 0.0004 0.0001 0.0004 0 0 0.0005 0.0001 0
Bud Neck 0.001 0.0002 0.001 0.0003 0.0045 0.0017 0.001 0.0002 0.0021 0.0009 0.0006 0.0026 0.0003 0.0002 0.0001 0.0028 0.001 0.0009
Bud Periphery 0.0005 0 0.0004 0.0001 0.0017 0 0.0013 0 0.001 0.0007 0.0005 0.0003 0.0004 0 0 0.0021 0.0002 0
Bud Site 0.0008 0.0001 0.0192 0.0012 0.002 0.0001 0.0044 0.0001 0.0075 0.005 0.0009 0.0002 0.0004 0.0002 0.0001 0.0023 0.0005 0.0001
Cell Periphery 0.0002 0 0.0002 0 0.0005 0 0.0002 0 0.0006 0.0001 0.0001 0.0001 0.0001 0 0 0.0006 0 0
Cytoplasm 0.0061 0.0004 0.0009 0.0034 0.0053 0.0002 0.002 0.0005 0.0011 0.001 0.0039 0.0002 0.0114 0.0002 0.0007 0.0034 0.0001 0.0004
Cytoplasmic Foci 0.0033 0 0.0005 0.0093 0.003 0.0007 0.0181 0.0007 0.0058 0.0063 0.017 0.0003 0.004 0.0001 0 0.0028 0 0
Eisosomes 0.0001 0 0.0002 0.0002 0.0016 0 0.0001 0 0.0003 0.0004 0.0001 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0049 0.0001 0.0001 0.0001 0.0034 0 0.0011 0.0001 0.0005 0.0003 0.0017 0.0001 0.0072 0 0 0.0006 0 0
Endosome 0.0077 0.0001 0.0004 0.0018 0.0217 0.0002 0.0182 0.0031 0.0065 0.012 0.0572 0.001 0.1043 0 0 0.0046 0.0001 0
Golgi 0.0016 0 0.0004 0.0029 0.0232 0.0004 0.0098 0.0042 0.004 0.009 0.0169 0.0008 0.0046 0 0 0.0018 0 0
Lipid Particles 0.0055 0 0.0004 0.0051 0.0046 0.0032 0.0178 0.0003 0.0094 0.002 0.0235 0.0012 0.0011 0 0 0.0049 0.0001 0
Mitochondria 0.0188 0.0001 0.0016 0.0106 0.008 0.0023 0.017 0.0008 0.0175 0.0119 0.0064 0.0025 0.0041 0.0001 0.0002 0.0179 0.0005 0.0001
None 0.0136 0.0001 0.0126 0.0506 0.0061 0.0002 0.0065 0.0001 0.0016 0.0004 0.0007 0 0.0015 0.0001 0.0296 0.0006 0.0004 0.0017
Nuclear Periphery 0.0082 0.0031 0.0013 0.0009 0.014 0.0005 0.0052 0.0013 0.0012 0.0006 0.0056 0.0005 0.0171 0.0012 0.0013 0.0017 0.001 0.0008
Nucleolus 0.0252 0.0309 0.0201 0.0181 0.0214 0.0525 0.0337 0.0162 0.0195 0.0159 0.0394 0.0612 0.0812 0.017 0.0236 0.0229 0.1299 0.0547
Nucleus 0.8721 0.9632 0.9266 0.8392 0.7789 0.9175 0.8355 0.9698 0.8965 0.8524 0.7441 0.9189 0.735 0.9391 0.9372 0.9015 0.8557 0.9379
Peroxisomes 0.0069 0 0.0008 0.0342 0.0015 0.011 0.0137 0.0001 0.0084 0.0093 0.0276 0.0002 0.0002 0 0 0.0115 0.0001 0
Punctate Nuclear 0.0138 0.0015 0.003 0.0109 0.008 0.0086 0.0038 0.0012 0.006 0.0099 0.028 0.0086 0.0019 0.0416 0.0068 0.0092 0.0098 0.0034
Vacuole 0.0018 0.0002 0.0002 0.0001 0.0023 0.0004 0.0025 0.0001 0.002 0.0016 0.0035 0.0005 0.0132 0.0001 0.0001 0.0068 0.0001 0.0001
Vacuole Periphery 0.0007 0.0001 0.0001 0.0001 0.0012 0 0.001 0.0001 0.0006 0.0004 0.0022 0.0002 0.0111 0 0 0.0012 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.2734 8.8736 28.5912 32.8794 13.8861 7.5713 16.2325 23.0272 30.4867 17.5682
Translational Efficiency 1.1467 0.9721 0.8301 0.8607 0.6792 1.4218 0.8786 0.6083 0.5754 0.5934

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
867 1319 107 856 947 1919 154 1263 1814 3238 261 2119

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 590.66 785.57 1052.28 883.26 654.67 745.04 963.83 832.36 624.08 761.55 1000.09 852.92
Standard Deviation 80.65 99.66 133.12 117.97 74.75 107.00 121.36 128.82 83.95 105.96 133.59 127.03
Intensity Change Log 2 — 0.411412 0.833119 0.580510 — 0.186550 0.558011 0.346440 — 0.297579 0.695046 0.462202

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000172 0.000608 0.001758 0.001484 0.000259 0.000504 0.001837 0.001008 0.000217 0.000547 0.001805 0.001200
Bud Neck 0.003046 0.010199 0.005059 0.043008 0.003355 0.013914 0.005281 0.021843 0.003207 0.012400 0.005190 0.030393
Bud Site 0.001964 0.001714 0.001827 0.025013 0.000615 0.003225 0.001622 0.006070 0.001260 0.002610 0.001706 0.013722
Cell Periphery 0.000189 0.000074 0.000150 0.000219 0.000119 0.000129 0.000098 0.000178 0.000152 0.000106 0.000119 0.000195
Cytoplasm 0.092638 0.020374 0.013919 0.031168 0.028428 0.023940 0.009649 0.028782 0.059117 0.022488 0.011400 0.029746
Cytoplasmic Foci 0.012817 0.004088 0.002797 0.004328 0.004880 0.006528 0.001218 0.001460 0.008674 0.005534 0.001865 0.002618
Eisosomes 0.000041 0.000028 0.000113 0.000039 0.000029 0.000036 0.000084 0.000031 0.000035 0.000033 0.000096 0.000034
Endoplasmic Reticulum 0.001625 0.003585 0.010499 0.005859 0.002037 0.003757 0.008905 0.004861 0.001840 0.003687 0.009559 0.005264
Endosome 0.000977 0.000837 0.001039 0.008990 0.000561 0.002762 0.000573 0.002369 0.000760 0.001978 0.000764 0.005044
Golgi 0.000147 0.001085 0.000170 0.007607 0.000579 0.001712 0.000155 0.000962 0.000373 0.001457 0.000161 0.003647
Lipid Particles 0.002852 0.001398 0.002775 0.003643 0.001301 0.003061 0.001100 0.000235 0.002042 0.002383 0.001787 0.001612
Mitochondria 0.001411 0.003700 0.001076 0.015370 0.002529 0.012901 0.000957 0.001771 0.001995 0.009153 0.001006 0.007265
Mitotic Spindle 0.001124 0.005390 0.002306 0.060555 0.001069 0.012338 0.000419 0.012152 0.001096 0.009508 0.001193 0.031705
None 0.033991 0.008683 0.003359 0.009396 0.018413 0.006176 0.004887 0.017966 0.025859 0.007197 0.004261 0.014504
Nuclear Periphery 0.002384 0.001795 0.000811 0.003954 0.000707 0.003817 0.000572 0.001028 0.001508 0.002993 0.000670 0.002210
Nuclear Periphery Foci 0.001141 0.000606 0.000775 0.001192 0.000528 0.001021 0.000470 0.000398 0.000821 0.000852 0.000595 0.000719
Nucleolus 0.014271 0.022791 0.007199 0.004428 0.022220 0.023319 0.010351 0.011242 0.018421 0.023104 0.009059 0.008490
Nucleus 0.824890* 0.909069* 0.932862* 0.756990* 0.909083* 0.869845* 0.946262* 0.881025* 0.868843* 0.885823* 0.940769* 0.830919*
Peroxisomes 0.001104 0.002121 0.000831 0.000924 0.002195 0.003302 0.000834 0.000306 0.001674 0.002821 0.000833 0.000556
Vacuole 0.002980 0.001381 0.010442 0.012126 0.000919 0.005931 0.004558 0.004985 0.001904 0.004077 0.006970 0.007870
Vacuole Periphery 0.000236 0.000475 0.000233 0.003706 0.000175 0.001782 0.000168 0.001328 0.000204 0.001250 0.000195 0.002288

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.13 -8.34 -13.78 -7.66 2.65 -3.91 -6.71 -16.41 -8.20 3.74 -5.78 -10.23 -20.61 -11.00 4.60
Bud Neck -8.13 -4.95 -14.93 -11.39 -11.92 -8.87 -5.39 -16.32 -7.96 -10.38 -11.70 -7.27 -22.03 -13.56 -15.72
Bud Site 0.09 -1.46 -5.73 -6.50 -5.41 -5.87 -5.85 -5.54 -2.78 -2.92 -2.44 -3.46 -7.54 -6.72 -6.01
Cell Periphery 6.62 0.47 0.77 -8.19 0.12 -0.68 0.24 -1.77 -0.77 -1.92 3.26 0.80 -0.37 -4.22 -1.25
Cytoplasm 11.56 11.39 10.56 -1.47 -2.82 1.34 2.50 1.03 -0.21 -1.94 10.40 9.53 9.29 -1.03 -3.04
Cytoplasmic Foci 4.37 5.76 6.36 3.09 1.03 -0.31 4.04 4.15 6.89 0.29 2.90 7.11 7.57 7.41 0.97
Eisosomes 3.81 -6.69 -5.40 -10.45 4.82 -1.98 -5.66 -3.75 -1.62 4.81 1.26 -8.40 -5.99 -8.17 6.73
Endoplasmic Reticulum -5.28 -8.50 -16.35 -12.94 2.29 -6.37 -8.73 -14.98 -6.93 4.48 -8.12 -12.12 -21.59 -13.90 4.83
Endosome 0.79 -1.03 -2.51 -2.71 -2.11 -4.04 -0.86 -1.44 1.76 -1.17 -3.57 -1.13 -2.71 -0.18 -2.31
Golgi -1.57 -2.73 -2.09 -0.85 -1.97 -2.03 1.47 0.20 2.12 -1.10 -2.68 1.08 -1.79 0.62 -2.25
Lipid Particles 2.95 0.70 1.82 0.14 1.12 -3.12 1.83 6.32 6.39 3.77 -0.68 1.75 4.06 4.25 2.26
Mitochondria -3.95 0.07 -3.99 -2.02 -4.02 -6.32 1.87 0.88 7.50 -5.86 -7.29 1.84 -2.73 5.19 -4.80
Mitotic Spindle -2.71 -1.15 -8.33 -7.48 -7.74 -5.59 -1.45 -7.44 -2.82 -3.33 -6.17 -1.70 -11.06 -7.46 -7.54
None 6.46 8.14 7.29 1.67 -3.07 6.08 6.90 2.93 -3.43 -4.54 8.76 10.49 7.34 -2.09 -5.43
Nuclear Periphery 1.02 2.03 -1.10 -3.44 -5.23 -6.77 -1.92 -9.77 4.30 -6.17 -3.03 1.71 -2.22 1.39 -7.24
Nuclear Periphery Foci 1.00 0.40 0.54 -0.91 0.21 -1.54 -0.22 0.62 1.90 0.60 0.07 0.37 0.82 1.03 0.58
Nucleolus -4.86 4.15 5.57 11.20 1.16 0.12 5.04 5.88 7.31 -0.02 -2.94 6.04 7.45 12.18 0.39
Nucleus -8.45 -7.79 2.78 11.38 10.29 4.65 -1.52 4.60 0.05 4.69 -2.86 -6.37 4.15 7.66 9.90
Peroxisomes -1.35 0.80 1.96 2.63 2.86 -0.75 1.80 2.59 5.12 2.39 -1.47 1.94 3.14 5.69 3.27
Vacuole 3.13 -3.29 -8.59 -9.58 -4.64 -5.47 -4.41 -6.99 -3.23 -0.67 -2.15 -5.20 -10.56 -9.69 -4.09
Vacuole Periphery -3.20 -1.89 -2.57 -1.78 -2.34 -4.88 -1.35 -1.48 2.46 -1.35 -5.29 -2.08 -2.66 1.40 -2.44
Standard name
Human Ortholog
Description TFIID subunit (150 kDa); involved in RNA polymerase II transcription initiation
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Taf2

Taf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Taf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available