Standard name | |
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Human Ortholog | |
Description | TFIID subunit (150 kDa); involved in RNA polymerase II transcription initiation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Bud | 0 | 0 | 0 | 2 | 8 | 6 | 13 | 19 | 9 | 8 | 6 | 21 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud Site | 2 | 0 | 1 | 4 | 15 | 19 | 51 | 31 | 15 | 43 | 29 | 54 | 0 | 1 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 7 | 7 | 8 | 12 | 18 | 31 | 32 | 22 | 25 | 12 | 18 | 23 | 78 | 111 | 18 | 14 | 15 | 0 | 1 | 2 | 0 | 2 | 1 |
Endoplasmic Reticulum | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 3 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 10 | 10 | 1 | 1 | 2 | 2 | 4 | 11 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 |
Mitochondria | 35 | 56 | 125 | 184 | 220 | 306 | 438 | 527 | 425 | 542 | 365 | 612 | 13 | 38 | 152 | 29 | 12 | 32 | 1 | 1 | 2 | 0 | 2 | 5 |
Nucleus | 75 | 89 | 125 | 193 | 422 | 302 | 460 | 522 | 349 | 471 | 290 | 501 | 283 | 495 | 557 | 177 | 140 | 136 | 77 | 88 | 109 | 113 | 355 | 516 |
Nuclear Periphery | 1 | 2 | 0 | 0 | 3 | 0 | 8 | 1 | 2 | 1 | 2 | 1 | 0 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 |
Nucleolus | 13 | 9 | 2 | 2 | 17 | 5 | 16 | 25 | 23 | 27 | 16 | 51 | 6 | 6 | 17 | 5 | 1 | 5 | 12 | 7 | 20 | 12 | 44 | 136 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 12 | 7 | 16 | 5 | 22 | 28 | 17 | 24 | 5 | 9 | 6 | 7 | 0 | 6 | 7 | 7 | 1 | 1 | 5 | 2 | 10 | 18 |
Vac/Vac Membrane | 0 | 4 | 0 | 0 | 7 | 3 | 4 | 8 | 3 | 0 | 2 | 3 | 1 | 2 | 5 | 8 | 7 | 10 | 2 | 6 | 21 | 5 | 31 | 49 |
Unique Cell Count | 85 | 112 | 171 | 246 | 503 | 414 | 671 | 767 | 528 | 688 | 436 | 737 | 310 | 586 | 704 | 219 | 173 | 188 | 99 | 112 | 171 | 143 | 462 | 773 |
Labelled Cell Count | 130 | 167 | 272 | 401 | 724 | 665 | 1045 | 1196 | 867 | 1141 | 728 | 1272 | 332 | 629 | 852 | 260 | 195 | 224 | 99 | 112 | 171 | 143 | 462 | 773 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 4.0 | 3.4 | 3.5 | 3.8 | 3.5 | 3.3 | 3.3 | 3.0 | 3.0 | 3.1 | 2.7 | 5.3 | 5.0 | 4.4 | 5.7 | 6.0 | 6.7 | 5.1 | 5.0 | 5.2 |
Std Deviation (1e-4) | 0.6 | 0.8 | 0.6 | 0.8 | 0.9 | 1.1 | 1.1 | 0.9 | 1.0 | 1.2 | 1.1 | 0.7 | 1.1 | 1.2 | 0.9 | 1.0 | 0.8 | 1.4 | 1.1 | 1.1 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.13 | 0.03 | -0.07 | -0.08 | -0.2 | -0.18 | -0.16 | -0.32 | 0.64 | 0.55 | 0.35 | 0.74 | 0.81 | 0.97 | 0.57 | 0.54 | 0.6 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5262 | 1.0424 | 0.5591 | 0.7243 | 0.4544 | 0.9166 | 1.2095 | 1.8813 | 1.855 | 1.4166 | 1.4142 | 1.5992 | 0.6007 | -0.2408 | -0.096 | 0.0581 | -0.6175 | -0.1573 |
Actin | 0.0069 | 0 | 0.0097 | 0.0107 | 0.0863 | 0.0004 | 0.0064 | 0.0009 | 0.0055 | 0.0595 | 0.0196 | 0.0006 | 0.0005 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 |
Bud | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0008 | 0 | 0.0007 | 0 | 0.0024 | 0.0005 | 0.0004 | 0.0001 | 0.0004 | 0 | 0 | 0.0005 | 0.0001 | 0 |
Bud Neck | 0.001 | 0.0002 | 0.001 | 0.0003 | 0.0045 | 0.0017 | 0.001 | 0.0002 | 0.0021 | 0.0009 | 0.0006 | 0.0026 | 0.0003 | 0.0002 | 0.0001 | 0.0028 | 0.001 | 0.0009 |
Bud Periphery | 0.0005 | 0 | 0.0004 | 0.0001 | 0.0017 | 0 | 0.0013 | 0 | 0.001 | 0.0007 | 0.0005 | 0.0003 | 0.0004 | 0 | 0 | 0.0021 | 0.0002 | 0 |
Bud Site | 0.0008 | 0.0001 | 0.0192 | 0.0012 | 0.002 | 0.0001 | 0.0044 | 0.0001 | 0.0075 | 0.005 | 0.0009 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0023 | 0.0005 | 0.0001 |
Cell Periphery | 0.0002 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0006 | 0 | 0 |
Cytoplasm | 0.0061 | 0.0004 | 0.0009 | 0.0034 | 0.0053 | 0.0002 | 0.002 | 0.0005 | 0.0011 | 0.001 | 0.0039 | 0.0002 | 0.0114 | 0.0002 | 0.0007 | 0.0034 | 0.0001 | 0.0004 |
Cytoplasmic Foci | 0.0033 | 0 | 0.0005 | 0.0093 | 0.003 | 0.0007 | 0.0181 | 0.0007 | 0.0058 | 0.0063 | 0.017 | 0.0003 | 0.004 | 0.0001 | 0 | 0.0028 | 0 | 0 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0016 | 0 | 0.0001 | 0 | 0.0003 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0049 | 0.0001 | 0.0001 | 0.0001 | 0.0034 | 0 | 0.0011 | 0.0001 | 0.0005 | 0.0003 | 0.0017 | 0.0001 | 0.0072 | 0 | 0 | 0.0006 | 0 | 0 |
Endosome | 0.0077 | 0.0001 | 0.0004 | 0.0018 | 0.0217 | 0.0002 | 0.0182 | 0.0031 | 0.0065 | 0.012 | 0.0572 | 0.001 | 0.1043 | 0 | 0 | 0.0046 | 0.0001 | 0 |
Golgi | 0.0016 | 0 | 0.0004 | 0.0029 | 0.0232 | 0.0004 | 0.0098 | 0.0042 | 0.004 | 0.009 | 0.0169 | 0.0008 | 0.0046 | 0 | 0 | 0.0018 | 0 | 0 |
Lipid Particles | 0.0055 | 0 | 0.0004 | 0.0051 | 0.0046 | 0.0032 | 0.0178 | 0.0003 | 0.0094 | 0.002 | 0.0235 | 0.0012 | 0.0011 | 0 | 0 | 0.0049 | 0.0001 | 0 |
Mitochondria | 0.0188 | 0.0001 | 0.0016 | 0.0106 | 0.008 | 0.0023 | 0.017 | 0.0008 | 0.0175 | 0.0119 | 0.0064 | 0.0025 | 0.0041 | 0.0001 | 0.0002 | 0.0179 | 0.0005 | 0.0001 |
None | 0.0136 | 0.0001 | 0.0126 | 0.0506 | 0.0061 | 0.0002 | 0.0065 | 0.0001 | 0.0016 | 0.0004 | 0.0007 | 0 | 0.0015 | 0.0001 | 0.0296 | 0.0006 | 0.0004 | 0.0017 |
Nuclear Periphery | 0.0082 | 0.0031 | 0.0013 | 0.0009 | 0.014 | 0.0005 | 0.0052 | 0.0013 | 0.0012 | 0.0006 | 0.0056 | 0.0005 | 0.0171 | 0.0012 | 0.0013 | 0.0017 | 0.001 | 0.0008 |
Nucleolus | 0.0252 | 0.0309 | 0.0201 | 0.0181 | 0.0214 | 0.0525 | 0.0337 | 0.0162 | 0.0195 | 0.0159 | 0.0394 | 0.0612 | 0.0812 | 0.017 | 0.0236 | 0.0229 | 0.1299 | 0.0547 |
Nucleus | 0.8721 | 0.9632 | 0.9266 | 0.8392 | 0.7789 | 0.9175 | 0.8355 | 0.9698 | 0.8965 | 0.8524 | 0.7441 | 0.9189 | 0.735 | 0.9391 | 0.9372 | 0.9015 | 0.8557 | 0.9379 |
Peroxisomes | 0.0069 | 0 | 0.0008 | 0.0342 | 0.0015 | 0.011 | 0.0137 | 0.0001 | 0.0084 | 0.0093 | 0.0276 | 0.0002 | 0.0002 | 0 | 0 | 0.0115 | 0.0001 | 0 |
Punctate Nuclear | 0.0138 | 0.0015 | 0.003 | 0.0109 | 0.008 | 0.0086 | 0.0038 | 0.0012 | 0.006 | 0.0099 | 0.028 | 0.0086 | 0.0019 | 0.0416 | 0.0068 | 0.0092 | 0.0098 | 0.0034 |
Vacuole | 0.0018 | 0.0002 | 0.0002 | 0.0001 | 0.0023 | 0.0004 | 0.0025 | 0.0001 | 0.002 | 0.0016 | 0.0035 | 0.0005 | 0.0132 | 0.0001 | 0.0001 | 0.0068 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0 | 0.001 | 0.0001 | 0.0006 | 0.0004 | 0.0022 | 0.0002 | 0.0111 | 0 | 0 | 0.0012 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.2734 | 8.8736 | 28.5912 | 32.8794 | 13.8861 | 7.5713 | 16.2325 | 23.0272 | 30.4867 | 17.5682 |
Translational Efficiency | 1.1467 | 0.9721 | 0.8301 | 0.8607 | 0.6792 | 1.4218 | 0.8786 | 0.6083 | 0.5754 | 0.5934 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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