Standard name
Human Ortholog
Description Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0
Cytoplasm 0.74 0.74 0.8 0.74 0.74 0.62 0.6 0.59 – 0.5 0.53 0.42 0.81 0.93 0.98 – 0.92 0.86 0.3 0.33 0.14 0.26 0.33 0.42
Endoplasmic Reticulum 0.19 0.17 0.07 0.12 0 0 0 0 – 0 0 0 0.15 0.07 0 – 0 0 0.37 0.22 0.57 0.32 0.23 0.2
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0.08 0.18 0.18 0.5 0.51 0.56 – 0.67 0.64 0.7 0 0 0 – 0 0 0.13 0.13 0.14 0.24 0.24 0.16
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0 0.05 0 – 0 0 0 0 0 0 – 0 0.05 0.05 0.14 0 0.05 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 3 0 0 0 0 1 – 0 0 0 1 0 1 – 2 3 12 12 16 12 16 16
Bud 3 2 4 6 2 4 11 8 – 6 18 22 3 0 0 – 0 1 5 2 4 2 6 5
Bud Neck 0 0 1 0 0 0 0 0 – 2 0 0 8 4 4 – 0 2 8 7 4 3 8 10
Bud Site 0 0 0 0 0 2 0 1 – 5 1 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 5 2 7 7 9 7 3 5 – 3 5 10 16 11 12 – 2 11 2 5 4 7 13 13
Cytoplasm 197 185 208 159 224 201 217 342 – 193 264 233 720 864 876 – 210 159 118 106 46 88 134 171
Endoplasmic Reticulum 50 42 19 25 7 5 3 8 – 3 2 6 133 63 14 – 8 7 145 71 186 109 92 81
Endosome 2 0 6 3 13 3 1 2 – 0 1 3 6 3 1 – 4 2 10 7 5 6 4 5
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 6 2 1 3 0 0
Mitochondria 10 21 22 38 56 163 185 326 – 258 315 387 2 0 0 – 9 8 50 42 45 82 97 65
Nucleus 0 1 0 0 0 4 1 2 – 0 2 3 2 0 2 – 2 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 1 – 1 1 8 0 0 0 – 0 0 0 2 0 0 1 0
Nucleolus 1 0 1 1 1 0 2 3 – 0 2 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 0 0 1 – 0 0 0 0 0 0 – 0 0 2 0 1 0 0 0
SpindlePole 0 0 0 0 0 1 1 5 – 4 3 5 3 3 0 – 0 0 3 2 0 0 2 4
Vac/Vac Membrane 10 4 11 9 25 14 17 20 – 13 16 20 39 16 13 – 9 10 20 46 8 17 21 29
Unique Cell Count 267 249 259 215 304 326 361 580 385 495 554 889 933 898 229 185 393 321 330 339 404 407
Labelled Cell Count 281 260 282 248 339 404 441 725 488 630 698 933 964 923 246 204 393 321 330 339 404 407


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.8 4.5 4.7 4.6 3.6 3.4 3.6 – 3.0 3.3 3.2 6.3 6.1 6.3 4.6 5.6 5.6 6.2 5.9 6.0
Std Deviation (1e-4) 0.7 0.7 1.2 1.4 1.9 1.2 1.1 1.0 – 0.7 1.0 0.7 0.8 0.7 1.0 0.9 0.9 0.9 1.3 1.3 1.4
Intensity Change (Log2) – – – 0.07 0.02 -0.33 -0.41 -0.33 – -0.6 -0.46 -0.5 0.48 0.43 0.48 0.03 0.31 0.31 0.46 0.4 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – -0.2 0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4981 2.1092 2.2744 1.954 1.954 2.134 3.7044 4.0179 3.8354 3.7977 3.0923 3.5524 3.0917 3.6712 3.7116 3.3898 3.2886 3.4842
Actin 0.0284 0.0074 0.0113 0.0235 0.0062 0.0036 0.0657 0.0037 0.0069 0.0004 0.0102 0.0009 0.0033 0.0011 0.0017 0.0067 0.0013 0.0018
Bud 0.0064 0.0113 0.0192 0.0238 0.004 0.0044 0.001 0.001 0.0005 0.0016 0.0001 0.0001 0.0006 0.0078 0.0006 0.0078 0.0002 0.0001
Bud Neck 0.0035 0.0017 0.0034 0.003 0.0156 0.0166 0.031 0.0006 0.0005 0.0002 0.0022 0.0009 0.0015 0.0007 0.0021 0.0288 0.0012 0.004
Bud Periphery 0.0058 0.0048 0.0096 0.0066 0.0039 0.003 0.002 0.0016 0.0011 0.0026 0.0002 0.0002 0.0018 0.0027 0.0015 0.0335 0.0002 0.0002
Bud Site 0.0056 0.0539 0.0418 0.0294 0.0425 0.0036 0.0097 0.0143 0.009 0.0004 0.0009 0.0002 0.0026 0.0095 0.0021 0.0051 0.0002 0.0003
Cell Periphery 0.0012 0.0026 0.0025 0.0011 0.0015 0.0009 0.0014 0.0016 0.0018 0.0003 0.0008 0.0006 0.0036 0.0016 0.0079 0.004 0.0003 0.0004
Cytoplasm 0.1606 0.3557 0.2879 0.2249 0.3841 0.3028 0.2871 0.3631 0.4113 0.362 0.4009 0.4668 0.4158 0.4221 0.4313 0.3227 0.4164 0.437
Cytoplasmic Foci 0.0272 0.0218 0.0164 0.0342 0.0226 0.0329 0.0248 0.0216 0.0175 0.0135 0.0407 0.0229 0.0212 0.0196 0.0179 0.017 0.02 0.0243
Eisosomes 0.0007 0.0006 0.0005 0.0006 0.0003 0.0002 0.0008 0.0003 0.0002 0.0003 0.0003 0.0001 0.0008 0.0002 0.0002 0.0006 0.0001 0.0001
Endoplasmic Reticulum 0.0877 0.0893 0.1015 0.1005 0.0181 0.0815 0.1351 0.1846 0.2095 0.0704 0.1319 0.183 0.2516 0.1644 0.2092 0.2535 0.2356 0.2383
Endosome 0.0219 0.0243 0.0156 0.0383 0.0326 0.0158 0.0612 0.0322 0.0265 0.0169 0.0913 0.0461 0.039 0.0377 0.0394 0.0219 0.0484 0.0383
Golgi 0.0113 0.0038 0.0037 0.0073 0.0182 0.001 0.0099 0.0019 0.003 0.0009 0.0025 0.0014 0.003 0.0021 0.0017 0.0014 0.0009 0.0012
Lipid Particles 0.0176 0.0046 0.0074 0.0358 0.0056 0.0038 0.0192 0.0069 0.0078 0.0015 0.0073 0.0054 0.007 0.0059 0.0044 0.0021 0.0019 0.0037
Mitochondria 0.0151 0.0043 0.0028 0.0046 0.028 0.0053 0.0066 0.0016 0.0031 0.0065 0.002 0.0009 0.0024 0.0024 0.0018 0.0026 0.0011 0.0014
None 0.5211 0.3277 0.4067 0.3805 0.3782 0.4035 0.2793 0.3136 0.2618 0.4996 0.2619 0.22 0.2077 0.2695 0.2321 0.2553 0.2004 0.1905
Nuclear Periphery 0.0121 0.015 0.0168 0.0242 0.0039 0.0164 0.0186 0.0122 0.0162 0.0028 0.0072 0.0114 0.0113 0.0103 0.0091 0.007 0.0048 0.0104
Nucleolus 0.0161 0.0053 0.0031 0.0024 0.0048 0.0024 0.001 0.0008 0.0003 0.0004 0.0006 0.0004 0.0004 0.0007 0.0005 0.0007 0.0002 0.0004
Nucleus 0.01 0.0332 0.0182 0.0186 0.0116 0.0242 0.0053 0.005 0.0035 0.0022 0.004 0.0048 0.0052 0.0081 0.0055 0.0068 0.0028 0.0132
Peroxisomes 0.0198 0.0035 0.0029 0.0096 0.0024 0.0008 0.0075 0.0004 0.0039 0.0002 0.0024 0.0004 0.0007 0.0004 0.0005 0.0018 0.0007 0.0004
Punctate Nuclear 0.0217 0.0194 0.0222 0.023 0.0101 0.0702 0.012 0.0024 0.002 0.0013 0.0073 0.0019 0.0031 0.0026 0.0022 0.0028 0.0009 0.0014
Vacuole 0.005 0.0084 0.0058 0.0059 0.005 0.0057 0.0171 0.028 0.0123 0.0102 0.0215 0.029 0.0156 0.0282 0.0253 0.0163 0.0568 0.03
Vacuole Periphery 0.0014 0.0014 0.001 0.0021 0.001 0.0015 0.004 0.0026 0.0013 0.0057 0.0039 0.0027 0.0017 0.0025 0.0029 0.0016 0.0056 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 52.973 51.1215 48.7157 41.7779 45.6578 53.5454 52.9131 47.7395 54.7273 64.4231
Translational Efficiency 0.6457 0.7084 0.6184 0.892 0.8933 0.6138 0.5671 0.752 0.6054 0.6062

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
83 1799 1767 118 1639 2061 2425 1062 1722 3860 4192 1180

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 892.82 795.06 890.71 1058.44 711.44 773.22 921.72 932.93 720.18 783.40 908.65 945.48
Standard Deviation 122.97 103.77 106.25 130.90 81.86 89.97 123.35 132.28 92.82 97.26 117.45 137.40
Intensity Change Log 2 — -0.167306 -0.003414 0.245498 — 0.120137 0.373586 0.391027 — -0.032725 0.176017 0.311853

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000305 0.000549 0.000458 0.018460 0.000244 0.000454 0.003489 0.005973 0.000247 0.000498 0.002212 0.007222
Bud Neck 0.003523 0.008899 0.002840 0.006820 0.007960 0.015750 0.012144 0.011001 0.007746 0.012557 0.008222 0.010583
Bud Site 0.051225 0.004936 0.006472 0.030251 0.001403 0.006055 0.017017 0.035973 0.003804 0.005534 0.012572 0.035401
Cell Periphery 0.000452 0.000308 0.000302 0.002701 0.000693 0.000427 0.002863 0.003073 0.000681 0.000372 0.001784 0.003036
Cytoplasm 0.219919* 0.421079* 0.509867* 0.166742* 0.359753* 0.366746* 0.292253* 0.307987* 0.353013* 0.392069* 0.383981* 0.293863*
Cytoplasmic Foci 0.097161 0.207339* 0.037385 0.071107 0.150250 0.210517* 0.019397 0.063318 0.147691 0.209036* 0.026979 0.064097
Eisosomes 0.000226 0.000516 0.000084 0.001636 0.001306 0.000947 0.001425 0.001758 0.001254 0.000746 0.000860 0.001746
Endoplasmic Reticulum 0.005660 0.048018 0.074027 0.220382* 0.142912* 0.062328 0.401470* 0.282210* 0.136297* 0.055659 0.263447* 0.276027*
Endosome 0.019969 0.014013 0.005564 0.016335 0.013497 0.018478 0.006757 0.011630 0.013809 0.016397 0.006254 0.012100
Golgi 0.012180 0.007502 0.000366 0.009673 0.016439 0.012521 0.003462 0.006311 0.016233 0.010182 0.002157 0.006647
Lipid Particles 0.011511 0.050422 0.007945 0.038485 0.140905* 0.087396 0.023150 0.041812 0.134668* 0.070164 0.016741 0.041479
Mitochondria 0.000899 0.005448 0.000473 0.002507 0.009967 0.011440 0.002696 0.001751 0.009530 0.008647 0.001759 0.001827
Mitotic Spindle 0.006153 0.000862 0.001305 0.001176 0.000111 0.000843 0.001181 0.003495 0.000402 0.000852 0.001233 0.003263
None 0.000469 0.003116 0.001618 0.000154 0.003415 0.001790 0.002030 0.000900 0.003273 0.002408 0.001856 0.000826
Nuclear Periphery 0.001388 0.003068 0.010595 0.004693 0.005335 0.003231 0.003118 0.001120 0.005145 0.003155 0.006270 0.001477
Nuclear Periphery Foci 0.005081 0.005005 0.038714 0.051142 0.002691 0.002745 0.016306 0.024116 0.002807 0.003798 0.025751 0.026818
Nucleolus 0.000308 0.001318 0.000289 0.000100 0.000689 0.000792 0.000305 0.000266 0.000671 0.001037 0.000298 0.000250
Nucleus 0.216547* 0.137051* 0.230780* 0.048801 0.094063 0.130658* 0.135365* 0.067250 0.099967 0.133638* 0.175584* 0.065405
Peroxisomes 0.001584 0.004607 0.000372 0.001170 0.006346 0.007894 0.001471 0.002595 0.006117 0.006362 0.001007 0.002453
Vacuole 0.337647* 0.074414 0.070288 0.304281* 0.040331 0.056921 0.053681 0.126322 0.054662 0.065074 0.060681 0.144118
Vacuole Periphery 0.007793 0.001529 0.000258 0.003384 0.001689 0.002065 0.000419 0.001137 0.001983 0.001815 0.000351 0.001362

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.13 -1.61 -2.38 -2.35 -2.36 -2.84 -7.23 -8.76 -8.41 -3.07 -2.52 -7.48 -7.48 -7.17 -5.08
Bud Neck -4.99 0.69 -2.71 1.97 -4.38 -5.73 -3.66 -2.31 3.15 0.97 -4.72 -0.69 -2.33 1.73 -2.06
Bud Site 2.11 2.03 0.50 -3.32 -3.14 -5.95 -11.24 -10.69 -9.06 -5.27 -2.06 -7.34 -10.24 -9.85 -7.25
Cell Periphery 0.96 0.98 -4.23 -4.39 -4.40 2.80 -9.16 -9.34 -11.25 -0.41 3.42 -7.09 -9.86 -12.18 -4.66
Cytoplasm -8.11 -11.23 1.34 13.51 17.81 -0.93 9.51 5.75 6.67 -1.64 -5.91 -4.13 6.72 12.30 10.77
Cytoplasmic Foci -9.27 3.54 1.03 16.31 -4.01 -10.90 33.20 18.27 30.03 -14.58 -13.09 31.62 18.27 36.58 -13.12
Eisosomes -4.15 2.18 -2.52 -1.85 -2.77 2.38 -0.57 -1.68 -3.90 -1.29 3.68 2.70 -1.90 -5.26 -4.42
Endoplasmic Reticulum -14.75 -17.91 -6.52 -4.79 -3.86 12.46 -27.85 -10.71 -19.56 10.64 13.83 -16.47 -11.07 -21.01 0.22
Endosome 0.41 2.95 0.33 -0.05 -4.46 -4.39 8.41 1.97 5.86 -5.00 -3.15 9.91 1.90 5.01 -6.56
Golgi 0.09 1.08 0.12 0.09 -1.51 3.70 14.95 9.80 6.74 -3.18 6.19 16.08 8.74 4.65 -4.84
Lipid Particles -11.92 0.45 -4.87 3.57 -6.09 11.12 28.46 21.36 12.91 -6.67 15.18 30.00 21.13 10.56 -10.09
Mitochondria -5.53 0.56 -0.90 2.41 -1.10 -1.20 8.95 9.39 9.53 2.05 0.94 10.32 9.31 9.40 0.17
Mitotic Spindle 1.28 1.23 1.13 -1.19 -0.75 -1.62 -2.27 -3.33 -2.41 -2.09 -0.22 -0.88 -2.57 -2.63 -2.19
None -3.37 -2.08 1.37 4.80 7.31 2.18 1.90 3.50 2.95 3.33 1.21 2.13 3.59 4.54 4.12
Nuclear Periphery -4.54 -10.30 -1.65 -0.56 3.67 3.46 4.50 8.99 5.27 10.63 3.92 -1.71 7.94 5.82 11.68
Nuclear Periphery Foci -0.54 -16.11 -7.45 -7.44 -1.10 -0.22 -16.43 -14.73 -14.63 -4.48 -3.66 -24.01 -16.41 -15.62 -0.17
Nucleolus -2.49 0.47 1.80 3.03 2.98 -1.38 6.78 7.23 9.78 1.63 -2.01 6.84 7.51 4.23 2.13
Nucleus 2.05 -0.98 4.72 9.42 18.49 -8.97 -9.71 5.92 13.77 14.20 -9.17 -18.70 7.50 17.58 26.19
Peroxisomes -6.51 2.30 0.26 9.41 -4.58 -2.70 13.16 9.66 10.87 -4.41 -0.60 15.03 9.93 11.81 -7.06
Vacuole 8.74 8.62 0.39 -11.20 -11.02 -6.34 -6.98 -17.17 -14.33 -13.91 -2.57 -3.18 -16.04 -15.94 -15.54
Vacuole Periphery 0.83 1.11 0.57 -0.54 -1.27 -1.77 9.48 1.97 2.96 -2.18 0.19 6.10 1.64 1.81 -2.70
Standard name
Human Ortholog
Description Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication
Localization
Cell Percentages ER (80%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Are1

Are1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Are1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available