Standard name
Human Ortholog
Description Threonine synthase; conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 1.0 0.92 0.99 0.95 0.9 0.95 0.92 0.88 0.89 1.0 0.99 1.0 0.99 0.99 0.99 0.97 0.97 0.93 0.95 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.07 0 0.06 0.07 0.05 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 0 0 2 1 4 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 0 0 0 1 0 0 1 1 0 0 1 2 1 0 2 3 1 0 0 0 0 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 1 1 0 2 4 9 4 8 7 3 0 1 0 2 4 3 0 0 1 0 0 0
Cytoplasm 340 300 215 104 131 203 214 289 213 201 142 141 321 391 431 477 389 288 300 287 190 217 245 273
Endoplasmic Reticulum 0 1 1 0 4 1 6 23 8 14 11 8 2 2 0 23 28 19 0 0 1 1 0 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Golgi 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 1 0 0 0 0
Mitochondria 0 0 0 0 2 0 0 0 0 0 3 2 0 0 0 1 0 2 0 0 0 0 1 0
Nucleus 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 2 1 0 0 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 3 0 4 0 3 3 0 0 0 1 1 0 1 1 2 1 0 1 0 1 3 4
Unique Cell Count 340 304 218 104 142 205 226 320 224 218 161 158 322 394 432 482 393 290 310 297 204 228 260 297
Labelled Cell Count 341 306 220 105 144 207 229 327 227 225 165 161 326 395 432 508 427 314 310 297 204 228 260 297


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 143.6 169.4 124.1 121.8 94.2 86.4 68.3 68.7 66.1 59.3 57.1 56.1 144.2 146.3 144.0 128.3 150.5 153.8 121.4 126.5 143.0
Std Deviation (1e-4) 25.0 36.9 41.9 31.7 30.3 23.2 17.6 18.7 17.6 15.5 15.0 16.8 30.6 27.0 29.8 38.6 44.2 47.9 30.7 33.2 38.2
Intensity Change (Log2) – – – -0.03 -0.4 -0.52 -0.86 -0.85 -0.91 -1.06 -1.12 -1.15 0.22 0.24 0.21 0.05 0.28 0.31 -0.03 0.03 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.2 -3.1 0.4 -2.3 -3.9 -2.1 -3.2 -4.3 -4.0 1.4 0.7 1.8 0.4 0.4 0.8
Endoplasmic Reticulum 0 0 0 0 3.7 0 3.4 3.5 0 0 0 0 2.9 3.7 3.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 125.6334 156.2862 143.938 137.4578 135.3881 144.2652 130.4521 150.2433 137.2113 135.3478 136.993 149.8731 118.8511 154.0901 144.2417 136.0855 122.7807 149.2238
Actin 0.0046 0.0002 0.0001 0.0001 0.0002 0.0002 0.0045 0.0001 0.0002 0 0 0 0.0044 0.0001 0.0069 0.0001 0.0013 0.0001
Bud 0.0005 0.0003 0 0 0.0001 0.0003 0.0002 0 0.0019 0 0 0 0.0027 0 0.0003 0.0013 0.0004 0
Bud Neck 0.0003 0.0002 0.0002 0.0005 0.0004 0.0004 0.0002 0.0001 0.0005 0.0001 0.0002 0.0001 0.0023 0.0001 0.0011 0.0002 0.0002 0.0003
Bud Periphery 0.0002 0.0001 0 0 0 0.0001 0.0001 0 0.0009 0 0 0 0.0012 0 0.0001 0.0004 0.0002 0
Bud Site 0.0002 0.0001 0 0 0.0002 0 0.0001 0 0.0006 0 0 0 0.001 0 0.0003 0.0002 0.0005 0
Cell Periphery 0.0016 0.0009 0.0001 0.0001 0.0004 0.0001 0.0001 0 0.0004 0 0 0 0.0009 0 0.0001 0.0001 0.0008 0
Cytoplasm 0.9281 0.9912 0.992 0.9874 0.9652 0.9651 0.9717 0.9977 0.9781 0.9971 0.9966 0.9943 0.9072 0.9972 0.962 0.964 0.8456 0.9954
Cytoplasmic Foci 0.0027 0.0001 0.0001 0 0.0018 0.0007 0.0013 0.0001 0.0015 0.0001 0.0001 0.0001 0.0108 0.0001 0.0053 0.0032 0.0225 0.0001
Eisosomes 0.0001 0 0 0 0.0005 0 0.0001 0 0 0 0 0 0.0001 0 0.0001 0 0.0006 0
Endoplasmic Reticulum 0.0008 0.0002 0.0001 0.0001 0.0001 0.0003 0.0004 0.0001 0.0001 0 0 0.0001 0.0015 0 0.0002 0.0001 0.0009 0.0001
Endosome 0.0013 0 0 0 0.0001 0.0004 0.0012 0 0.0001 0 0 0 0.0036 0 0.0016 0.0003 0.0034 0
Golgi 0.0011 0 0 0 0.0001 0 0.0009 0 0 0 0 0 0.0006 0 0.0008 0 0.0011 0
Lipid Particles 0.0013 0 0 0 0.001 0 0.0012 0 0.0005 0 0 0 0.0032 0 0.0026 0.0002 0.0101 0
Mitochondria 0.0014 0.0004 0.0001 0 0.0027 0.0008 0.0005 0 0.0022 0 0 0.0001 0.0038 0 0.0013 0.0076 0.0101 0
None 0.0021 0.0002 0.0011 0.0001 0.0015 0.0009 0.0009 0.0001 0.002 0.0001 0 0.0001 0.0089 0.0001 0.0039 0.0019 0.0377 0.0001
Nuclear Periphery 0.0107 0.0018 0.0012 0.0021 0.0028 0.0034 0.0021 0.0003 0.0008 0.0003 0.0004 0.0005 0.0053 0.0003 0.0013 0.0014 0.0032 0.0005
Nucleolus 0.0031 0.0001 0 0 0.0101 0.0001 0.0013 0 0.0037 0 0 0 0.0079 0 0.0021 0.0028 0.0342 0
Nucleus 0.0293 0.0035 0.0042 0.0075 0.0105 0.0229 0.009 0.0013 0.004 0.0022 0.0026 0.0044 0.021 0.0019 0.0038 0.0071 0.0118 0.0033
Peroxisomes 0.0008 0 0 0 0.0002 0 0.0029 0 0.0001 0 0 0 0.0005 0 0.0038 0.0004 0.0027 0
Punctate Nuclear 0.0013 0 0 0 0.0003 0.0001 0.0005 0 0.0002 0 0 0 0.0049 0 0.0016 0.0001 0.0049 0
Vacuole 0.0065 0.0007 0.0004 0.0016 0.0011 0.0026 0.0005 0 0.0013 0.0001 0 0.0001 0.006 0 0.0005 0.0036 0.0046 0
Vacuole Periphery 0.0019 0.0001 0.0002 0.0004 0.0008 0.0014 0.0003 0 0.0008 0 0 0 0.0021 0 0.0004 0.005 0.0032 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 270.6733 252.8187 276.6662 324.9833 267.1402 259.8167 322.7586 284.2816 280.0698 300.5266
Translational Efficiency 2.0541 2.3602 2.129 1.7305 2.0817 2.1923 1.7939 1.8887 2.0803 1.844

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
283 854 226 1975 1021 1353 238 572 1304 2207 464 2547

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 8196.30 7616.10 10048.96 8743.83 8662.73 8246.73 9328.24 8726.34 8561.50 8002.71 9679.28 8739.90
Standard Deviation 1485.72 2006.81 2037.18 2078.79 1518.30 2068.88 1573.71 2189.44 1523.47 2068.02 1849.72 2104.16
Intensity Change Log 2 — -0.105920 0.294002 0.093293 — -0.071000 0.106783 0.010555 — -0.087871 0.200839 0.051372

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000057 0.000156 0.000098 0.000202 0.000071 0.000047 0.000109 0.000169 0.000068 0.000089 0.000104 0.000195
Bud Neck 0.000513 0.000419 0.002334 0.001191 0.000512 0.000206 0.001715 0.001690 0.000512 0.000288 0.002016 0.001303
Bud Site 0.000359 0.002286 0.000648 0.003842 0.000363 0.000489 0.004100 0.009908 0.000362 0.001184 0.002419 0.005204
Cell Periphery 0.003674 0.001221 0.004299 0.000566 0.003914 0.000573 0.002842 0.001730 0.003862 0.000824 0.003551 0.000827
Cytoplasm 0.748597* 0.767810* 0.535261* 0.849308* 0.762117* 0.799329* 0.715262* 0.780375* 0.759183* 0.787133* 0.627589* 0.833827*
Cytoplasmic Foci 0.010398 0.006370 0.028624 0.004224 0.006909 0.003428 0.020213 0.014977 0.007667 0.004567 0.024310 0.006639
Eisosomes 0.000001 0.000002 0.000004 0.000002 0.000001 0.000001 0.000003 0.000003 0.000001 0.000001 0.000003 0.000002
Endoplasmic Reticulum 0.000011 0.000072 0.000016 0.000043 0.000020 0.000017 0.000014 0.000051 0.000019 0.000038 0.000015 0.000045
Endosome 0.000024 0.000260 0.000048 0.000465 0.000027 0.000010 0.000089 0.006869 0.000027 0.000107 0.000069 0.001903
Golgi 0.001213 0.000625 0.000853 0.000997 0.001339 0.000307 0.000541 0.009885 0.001312 0.000430 0.000693 0.002993
Lipid Particles 0.000907 0.000672 0.001440 0.000534 0.001698 0.000177 0.002883 0.001711 0.001526 0.000369 0.002180 0.000798
Mitochondria 0.000103 0.000160 0.000190 0.000305 0.000135 0.000094 0.000101 0.000955 0.000128 0.000120 0.000144 0.000451
Mitotic Spindle 0.000049 0.000054 0.000061 0.002188 0.000081 0.000095 0.000769 0.002893 0.000074 0.000079 0.000424 0.002347
None 0.020977 0.015685 0.006635 0.011910 0.013215 0.015141 0.014469 0.010912 0.014900 0.015352 0.010653 0.011686
Nuclear Periphery 0.000242 0.000093 0.000792 0.000129 0.000348 0.000064 0.000403 0.000174 0.000325 0.000075 0.000593 0.000139
Nuclear Periphery Foci 0.001780 0.000702 0.000932 0.000526 0.001956 0.000407 0.001577 0.001370 0.001918 0.000521 0.001263 0.000716
Nucleolus 0.000117 0.000153 0.000085 0.000271 0.000042 0.000053 0.000079 0.000188 0.000059 0.000092 0.000082 0.000252
Nucleus 0.202422* 0.195196* 0.398091* 0.102248 0.198097* 0.175603* 0.203658* 0.107311 0.199036* 0.183185* 0.298360* 0.103385
Peroxisomes 0.000003 0.000042 0.000005 0.000040 0.000001 0.000005 0.000008 0.000132 0.000002 0.000019 0.000006 0.000061
Vacuole 0.008187 0.007714 0.019330 0.020608 0.008801 0.003839 0.030993 0.039084 0.008667 0.005338 0.025312 0.024758
Vacuole Periphery 0.000367 0.000307 0.000255 0.000401 0.000351 0.000115 0.000171 0.009614 0.000354 0.000189 0.000212 0.002470

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.62 -4.84 -1.68 -0.09 0.51 2.82 -3.71 -3.20 -4.53 -0.31 -0.31 -5.71 -1.91 -1.60 0.35
Bud Neck 0.90 -5.65 -3.43 -6.04 4.94 5.45 -7.40 -5.04 -6.04 0.55 4.02 -7.87 -7.20 -9.80 5.51
Bud Site -2.92 -4.71 -6.43 -1.87 -3.25 -1.77 -3.51 -5.23 -5.11 -2.58 -3.42 -4.82 -8.64 -6.43 -2.59
Cell Periphery 4.36 -1.41 5.95 3.41 8.15 7.65 1.12 5.12 -7.00 4.36 8.34 -0.13 8.81 1.12 10.32
Cytoplasm -1.93 15.95 -7.16 -7.89 -24.23 -6.51 8.28 1.99 7.03 -6.43 -5.82 17.19 -8.13 -2.86 -22.44
Cytoplasmic Foci 2.45 -8.18 4.67 3.27 10.56 4.66 -7.25 -5.28 -8.11 5.15 4.45 -11.36 2.37 -2.37 12.09
Eisosomes 0.32 -7.00 0.28 -0.07 7.12 5.26 -5.92 -5.49 -9.00 2.14 3.60 -9.02 -1.38 -4.50 8.16
Endoplasmic Reticulum -1.08 -3.33 -2.64 0.71 0.54 0.74 1.10 -0.66 -1.19 -1.61 -0.72 -1.00 -1.40 0.28 -0.63
Endosome -1.05 -3.66 -1.97 -0.09 -1.00 4.86 -1.97 -2.44 -2.47 -2.34 -0.82 -3.62 -3.00 -2.57 -2.56
Golgi 2.08 0.66 2.07 0.25 1.98 8.29 4.79 -1.25 -2.13 -1.84 7.33 3.72 0.86 -2.02 -0.72
Lipid Particles 0.75 -3.70 0.26 -0.68 4.05 5.95 -3.16 -2.95 -8.39 1.52 5.20 -3.87 1.07 -6.63 4.73
Mitochondria -0.82 -4.21 -2.62 -1.31 0.95 4.48 4.57 -1.77 -2.14 -2.15 1.46 -1.91 -2.73 -3.15 -1.85
Mitotic Spindle -0.66 -2.33 -2.45 -2.43 -2.24 -0.39 -1.86 -1.88 -1.86 -0.57 -0.24 -1.97 -3.00 -2.98 -1.31
None 1.49 4.36 3.71 4.42 -1.79 -1.41 2.46 3.49 4.93 0.49 -0.23 5.54 5.47 6.93 -0.33
Nuclear Periphery 5.19 -3.66 3.11 -4.67 3.99 8.61 -4.57 2.29 -11.55 6.26 9.37 -4.45 5.39 -10.81 5.54
Nuclear Periphery Foci 3.11 0.74 3.36 0.41 2.60 7.41 -0.96 0.09 -11.31 1.19 7.61 0.21 5.00 -6.88 4.62
Nucleolus -0.17 -0.46 -1.19 -1.08 -1.72 -0.90 -6.21 -3.20 -3.00 -2.24 -0.74 -3.14 -4.72 -3.31 -3.17
Nucleus 0.99 -11.41 9.94 13.34 20.31 4.48 -1.57 12.36 9.36 9.89 3.95 -9.70 17.80 17.10 20.37
Peroxisomes -1.19 -4.89 -3.63 0.26 -2.37 -3.57 -4.96 -3.09 -2.84 -2.42 -1.45 -7.65 -4.93 -1.38 -3.28
Vacuole -0.07 -6.56 -10.81 -12.69 3.27 4.36 -7.95 -14.36 -15.44 -1.65 2.49 -10.35 -20.36 -22.51 3.24
Vacuole Periphery 0.57 0.07 0.74 0.24 1.75 3.73 2.51 -1.61 -1.78 -1.73 2.81 1.45 -1.30 -1.89 -1.60
Standard name
Human Ortholog
Description Threonine synthase; conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Thr4

Thr4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Thr4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available