Standard name
Human Ortholog
Description Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 1.0 0.96 0.95 0.88 0.8 0.75 – 0.69 0.73 0.66 0.67 1.0 0.98 0.98 1.0 0.94 0.92 0.97 0.94 0.95 0.93 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.11 0.08 0.21 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0.05 0 0.07 0 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0.07 0.15 0.23 0.24 – 0.24 0.19 0.22 0.19 0 0 0 0 0.07 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 1 0 0 0 0 0 – 1 0 2 6 0 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 0 5 0 6 – 1 11 5 9 0 0 0 2 1 3 0 0 0 0 0 0
Cytoplasm 150 206 327 208 273 242 403 – 229 301 282 373 289 364 418 248 197 281 147 194 319 97 279 365
Endoplasmic Reticulum 0 0 0 0 2 1 0 – 1 6 2 12 0 0 1 11 7 12 0 0 1 0 1 3
Endosome 0 0 0 0 0 2 1 – 0 1 1 2 1 0 0 0 1 0 0 2 0 0 0 0
Golgi 0 0 2 0 1 0 0 – 0 0 0 0 0 0 0 0 1 0 0 1 3 0 0 0
Mitochondria 0 1 0 1 1 6 11 – 38 33 89 79 0 0 0 2 3 1 0 1 1 0 0 1
Nucleus 5 2 5 2 6 3 14 – 14 22 16 39 2 2 4 7 12 9 0 2 3 2 3 2
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 0 3 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 – 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 18 16 46 69 131 – 81 78 96 108 1 8 14 0 14 34 4 2 1 1 1 3
Unique Cell Count 153 207 340 219 309 301 535 331 412 430 558 290 370 425 249 209 305 153 208 336 105 293 383
Labelled Cell Count 155 211 353 227 334 323 567 366 452 493 633 293 374 438 270 236 340 153 208 336 105 293 383


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.5 9.1 11.0 10.5 10.6 9.0 8.4 – 7.6 7.9 7.4 7.5 11.0 10.6 10.3 8.4 10.2 10.7 11.4 11.3 11.5
Std Deviation (1e-4) 1.6 1.9 1.6 1.6 1.5 1.4 1.2 – 1.2 1.2 1.2 1.2 1.6 1.5 1.5 1.3 1.8 2.3 1.5 1.8 1.7
Intensity Change (Log2) – – – -0.07 -0.06 -0.3 -0.39 – -0.54 -0.47 -0.56 -0.55 -0.0 -0.06 -0.1 -0.39 -0.12 -0.04 0.05 0.04 0.06

WT3RAP60RAP140RAP220RAP300RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 2.6 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -3.8 -6.3 -8.1 – -9.3 -8.5 -10.4 -10.3 3.0 1.8 1.9 2.7 -1.0 -2.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 3.7
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 6.4 5.3 8.9 7.3 0 0 0 0 0 0
Nucleus 0 0.5 0 1.1 – 2.2 2.8 1.9 3.7 0 0 0 1.1 2.8 1.3
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 1.0 4.1 6.5 7.4 – 7.0 5.6 6.6 5.9 -3.6 -2.2 -1.4 -3.7 0.7 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0011 5.1304 4.6823 3.7268 4.449 4.4724 6.4069 8.2098 7.1521 7.5925 7.43 7.3028 3.0859 4.4828 4.3847 3.9333 4.4472 3.9128
Actin 0.0193 0.0009 0.0151 0.0122 0.0011 0.0015 0.3553 0.0131 0.0084 0.0013 0.006 0.0369 0.0231 0.0002 0.0051 0.0008 0.0002 0.0205
Bud 0.0006 0.0003 0.0009 0.0024 0.0001 0.0016 0.0019 0.0003 0.0003 0.0002 0.0057 0.0012 0.0009 0.0001 0.0003 0.0001 0.0002 0.0001
Bud Neck 0.0014 0.0002 0.0016 0.0058 0.0005 0.0011 0.0011 0.0002 0.0092 0.0006 0.0021 0.0051 0.0075 0.0001 0.0011 0.0004 0.0004 0.0009
Bud Periphery 0.0005 0.0001 0.0007 0.0015 0 0.0007 0.0026 0.0002 0.0003 0 0.0034 0.0016 0.0012 0 0.0003 0 0 0.0001
Bud Site 0.0016 0.001 0.0021 0.0071 0.0002 0.0002 0.0183 0.0006 0.0171 0.0002 0.0223 0.0072 0.0157 0.0002 0.0054 0.0002 0.0001 0.0001
Cell Periphery 0.0001 0 0.0001 0.0005 0 0 0.0003 0.0001 0.0005 0 0.0004 0.0009 0.0008 0 0.0002 0 0 0.0001
Cytoplasm 0.8252 0.9654 0.9157 0.8222 0.9008 0.9016 0.3283 0.9215 0.925 0.9633 0.8136 0.9117 0.8175 0.9845 0.9403 0.9577 0.9046 0.9442
Cytoplasmic Foci 0.0203 0.0064 0.0069 0.0202 0.0306 0.0091 0.0355 0.0043 0.0016 0.0033 0.0111 0.005 0.0202 0.0012 0.0034 0.0038 0.0029 0.0029
Eisosomes 0.0001 0 0 0.0003 0 0 0.0014 0.0001 0.0001 0 0 0.0002 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0078 0.0014 0.0031 0.001 0.0013 0.0036 0.0013 0.0014 0.0038 0.0022 0.0009 0.0017 0.0028 0.0017 0.0021 0.0041 0.0018 0.0101
Endosome 0.0231 0.0017 0.0046 0.0049 0.0075 0.0085 0.0269 0.0156 0.001 0.0011 0.0052 0.0022 0.012 0.0012 0.0088 0.0031 0.0027 0.0031
Golgi 0.0047 0.0004 0.0029 0.0062 0.0053 0.0007 0.0304 0.0098 0.0004 0.0001 0.0056 0.0044 0.0112 0 0.0036 0.0001 0.0001 0.0007
Lipid Particles 0.0062 0 0.0011 0.0137 0.0029 0.0003 0.0774 0.0007 0.0002 0 0.0002 0.0009 0.0152 0 0.0048 0 0 0.0005
Mitochondria 0.003 0.0003 0.0088 0.0423 0.0009 0.0056 0.0282 0.002 0.0002 0.0002 0.0068 0.0023 0.0121 0.0001 0.0017 0.0001 0.0004 0.0002
None 0.0219 0.0091 0.0111 0.0081 0.0043 0.0286 0.0081 0.0245 0.0048 0.0073 0.0023 0.0032 0.0073 0.0042 0.0079 0.0156 0.0047 0.0036
Nuclear Periphery 0.0207 0.0011 0.0022 0.0016 0.0015 0.0031 0.0023 0.0015 0.0023 0.0022 0.0225 0.0008 0.0031 0.0009 0.0022 0.0026 0.0064 0.0019
Nucleolus 0.0007 0 0.0001 0.0098 0 0.0004 0.0012 0 0.0001 0.0001 0.0005 0.0001 0.0011 0 0.0001 0 0.0001 0
Nucleus 0.0229 0.0077 0.0116 0.0139 0.0101 0.0283 0.0011 0.0022 0.0235 0.0142 0.0853 0.011 0.0239 0.0045 0.0066 0.0093 0.0733 0.0092
Peroxisomes 0.0091 0.0016 0.0078 0.0221 0.0305 0.001 0.0688 0.0007 0.0001 0.0001 0.003 0.0006 0.0105 0 0.0037 0.0001 0 0.0002
Punctate Nuclear 0.0065 0.0018 0.0022 0.0013 0.0017 0.0026 0.0074 0.0007 0.0005 0.0031 0.0014 0.0007 0.0079 0.0002 0.0009 0.001 0.0009 0.0006
Vacuole 0.003 0.0005 0.001 0.0019 0.0005 0.0012 0.0013 0.0005 0.0005 0.0003 0.001 0.0021 0.0043 0.0007 0.0008 0.0008 0.0008 0.0006
Vacuole Periphery 0.0012 0.0001 0.0004 0.0014 0.0001 0.0003 0.001 0.0004 0.0001 0.0001 0.0007 0.0001 0.0016 0.0001 0.0004 0.0002 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 71.0041 55.8566 47.1 39.5504 58.9184 56.7719 54.2303 48.5136 47.7081 56.171
Translational Efficiency 0.5782 0.6214 0.6693 0.8659 0.5982 0.6764 0.6826 0.6932 0.7299 0.5731

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
24 486 306 191 1770 248 1921 1375 1794 734 2227 1566

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1031.05 1225.70 1327.13 1142.44 930.91 1178.43 1071.77 1066.99 932.25 1209.73 1106.86 1076.19
Standard Deviation 241.86 171.07 145.76 149.88 163.04 144.80 172.10 200.40 164.75 164.19 190.26 196.50
Intensity Change Log 2 — 0.249492 0.364195 0.148004 — 0.340152 0.203282 0.196833 — 0.293219 0.290077 0.171379

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001507 0.000365 0.000518 0.000489 0.000106 0.000459 0.000134 0.000560 0.000124 0.000397 0.000187 0.000551
Bud Neck 0.010962 0.001004 0.002552 0.002422 0.001061 0.001051 0.002600 0.002429 0.001194 0.001020 0.002593 0.002428
Bud Site 0.030364 0.004463 0.004048 0.005888 0.001832 0.006860 0.004958 0.021677 0.002214 0.005273 0.004833 0.019752
Cell Periphery 0.000250 0.000087 0.000191 0.000141 0.000061 0.000112 0.000546 0.000163 0.000064 0.000095 0.000498 0.000161
Cytoplasm 0.495072 0.785331 0.519204 0.446484 0.797456 0.756201 0.628077 0.690045 0.793411 0.775489 0.613118 0.660339
Cytoplasmic Foci 0.063806 0.042761 0.011775 0.018238 0.033672 0.064665 0.016436 0.013662 0.034075 0.050162 0.015795 0.014220
Eisosomes 0.000065 0.000012 0.000018 0.000037 0.000010 0.000027 0.000480 0.000015 0.000011 0.000017 0.000416 0.000017
Endoplasmic Reticulum 0.012264 0.000760 0.002118 0.001658 0.000641 0.001371 0.000815 0.001212 0.000797 0.000967 0.000994 0.001266
Endosome 0.020143 0.003358 0.001755 0.019983 0.001388 0.004684 0.002213 0.004854 0.001639 0.003806 0.002150 0.006699
Golgi 0.000948 0.003356 0.000194 0.009748 0.000497 0.002365 0.002344 0.004761 0.000503 0.003021 0.002049 0.005369
Lipid Particles 0.027982 0.000556 0.001470 0.001166 0.000431 0.000954 0.000807 0.000507 0.000800 0.000690 0.000898 0.000587
Mitochondria 0.005777 0.000326 0.000325 0.009096 0.000293 0.002215 0.000581 0.000631 0.000366 0.000964 0.000546 0.001663
Mitotic Spindle 0.010332 0.004872 0.011091 0.040507 0.000441 0.005649 0.008224 0.024504 0.000573 0.005135 0.008618 0.026456
None 0.001769 0.008787 0.007575 0.004974 0.010094 0.003294 0.009199 0.025077 0.009983 0.006931 0.008976 0.022625
Nuclear Periphery 0.004398 0.000267 0.001348 0.003123 0.000124 0.000214 0.000417 0.000455 0.000181 0.000249 0.000545 0.000780
Nuclear Periphery Foci 0.048476 0.005396 0.004945 0.007049 0.002262 0.003415 0.001124 0.002268 0.002880 0.004726 0.001649 0.002852
Nucleolus 0.010042 0.000345 0.000157 0.000221 0.000263 0.000656 0.000269 0.000335 0.000393 0.000450 0.000254 0.000321
Nucleus 0.132580 0.096993 0.338267 0.122587 0.122587 0.074833 0.217694 0.133802 0.122721 0.089506 0.234261 0.132434
Peroxisomes 0.004085 0.000760 0.000101 0.001313 0.000122 0.001308 0.000303 0.000213 0.000175 0.000945 0.000275 0.000347
Vacuole 0.113503 0.038886 0.092222 0.287405 0.026288 0.066107 0.102637 0.070248 0.027455 0.048084 0.101206 0.096734
Vacuole Periphery 0.005676 0.001315 0.000126 0.017471 0.000370 0.003560 0.000140 0.002582 0.000441 0.002073 0.000138 0.004398

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.57 1.95 2.55 -0.35 3.95 -2.12 -3.45 -5.04 0.45 -3.79 -3.05 -5.44 -5.28 0.12 -2.94
Bud Neck 2.17 1.55 1.81 -6.01 3.09 -0.02 -2.75 -2.63 -5.59 0.12 0.20 -2.93 -2.50 -7.14 1.00
Bud Site 1.78 1.73 1.66 -2.33 -1.53 -2.36 -4.22 -6.62 -1.77 -4.16 -4.04 -4.11 -6.65 -3.49 -4.25
Cell Periphery 4.13 0.49 3.69 -1.60 6.16 -3.10 -4.10 -5.13 0.20 3.15 -4.11 -4.56 -5.37 -0.89 3.52
Cytoplasm -3.77 0.79 2.01 19.83 3.95 3.99 29.45 26.11 9.82 0.53 4.02 32.78 29.31 19.99 1.51
Cytoplasmic Foci 1.41 3.56 3.70 9.76 1.40 -5.70 13.84 14.55 10.61 1.66 -4.98 14.36 15.14 14.07 1.94
Eisosomes 3.63 2.75 1.62 -9.49 -5.05 -3.75 -3.28 -7.62 2.31 3.15 -3.97 -3.31 -9.58 -0.63 3.12
Endoplasmic Reticulum 2.08 1.78 1.91 -4.80 2.62 -1.76 -1.24 -2.06 0.84 -1.03 -1.12 -1.63 -1.66 -0.35 -0.33
Endosome 1.71 1.82 1.66 -0.33 -1.31 -3.22 0.11 -1.41 2.22 -1.34 -3.43 0.71 -1.27 1.92 -1.66
Golgi -1.53 2.30 0.03 1.42 -1.20 -1.16 -1.02 -1.59 0.25 -0.65 -2.27 -0.91 -1.64 0.95 -0.79
Lipid Particles 1.69 1.61 1.62 -6.85 1.09 -2.40 -4.41 -4.34 0.92 0.94 0.20 -0.75 -0.32 -1.24 1.53
Mitochondria 1.06 1.04 0.93 -0.99 -0.80 -1.44 -0.68 -0.62 1.35 0.22 -1.04 -0.33 -0.52 0.85 -0.32
Mitotic Spindle 0.67 0.18 -1.25 -2.72 -1.96 -1.38 -5.84 -7.07 -2.23 -3.17 -2.49 -6.11 -7.73 -4.56 -3.69
None -4.43 -4.13 -2.85 0.55 -0.70 4.99 1.67 -1.88 -6.01 -3.45 2.55 1.89 -1.63 -4.02 -3.54
Nuclear Periphery 1.70 1.00 1.05 -6.52 0.48 -3.31 -19.43 -17.53 -11.59 -0.71 -1.18 -15.90 -13.87 -7.57 0.01
Nuclear Periphery Foci 1.48 1.51 1.54 2.30 1.31 -2.36 3.53 0.65 2.73 -3.56 -3.78 2.06 1.08 6.03 -2.23
Nucleolus 1.67 1.69 1.68 0.48 -1.22 -0.80 0.94 -0.27 0.76 -0.91 -0.20 1.89 1.29 0.92 -1.13
Nucleus 0.89 -5.68 0.10 -2.91 14.39 7.53 -18.25 -12.47 -16.73 3.59 6.31 -21.89 -11.80 -16.61 8.07
Peroxisomes 1.41 1.65 1.59 1.57 -1.24 -1.92 -1.56 -0.33 1.91 1.38 -2.70 0.05 0.83 2.86 0.84
Vacuole 1.87 -1.61 -8.92 -17.26 -11.50 -6.65 -25.01 -21.63 -6.99 -0.78 -7.99 -27.08 -25.31 -17.01 -5.36
Vacuole Periphery 1.18 1.46 1.04 -0.29 -1.14 -1.41 1.26 -1.52 1.09 -2.70 -1.72 1.55 -1.52 0.95 -2.91
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Yih1

Yih1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yih1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available