Standard name
Human Ortholog
Description Component of conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly of large protein complexes such as box C/D snoRNPs and RNA polymerase II; contains a single TPR domain with at least two TPR motifs; plays a role in determining prion variants
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.1 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.95 0.94 0.96 0.91 0.8 0.73 0.67 0.7 0.61 0.63 0.98 1.0 0.99 0.77 – 0.76 0.94 0.88 0.92 0.94 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.16 0.05 0.24 0.34 0.35 0.51 0.4 0.55 0.53 0 0 0 0 – 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.08 0 0.08 0.07 0 0 0 0.34 – 0.24 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0
Bud 0 0 7 5 0 1 3 4 4 3 10 8 1 2 0 0 – 0 1 1 1 0 0 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – 0 0 1 1 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 1 0 2 2 1 2 1 1 5 4 6 2 2 5 3 – 5 0 0 0 0 0 0
Cytoplasm 538 407 230 239 265 279 264 306 242 245 258 274 252 389 482 61 – 38 529 361 236 169 357 338
Endoplasmic Reticulum 3 2 0 0 2 2 2 0 1 2 3 2 1 0 2 1 – 0 0 1 1 0 4 5
Endosome 0 0 0 0 1 0 0 0 0 0 0 1 0 0 1 1 – 0 5 2 0 0 0 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 0 1 2 5 5
Mitochondria 4 0 14 40 13 73 113 147 184 140 232 234 1 0 0 0 – 1 3 3 1 0 4 1
Nucleus 9 9 4 0 2 1 2 13 5 10 10 13 5 0 0 1 – 2 6 17 5 3 2 5
Nuclear Periphery 0 0 0 1 0 0 0 1 2 0 0 4 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 2 0 1 0 0 1
Vac/Vac Membrane 3 8 5 4 3 5 10 31 28 13 32 31 1 0 0 27 – 12 9 11 3 2 5 5
Unique Cell Count 549 416 241 253 276 306 332 419 359 349 424 438 256 389 485 79 50 563 409 257 179 390 378
Labelled Cell Count 558 427 260 291 289 362 396 504 468 418 550 573 263 393 490 94 58 563 409 257 179 390 378


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.1 9.1 5.5 6.2 6.5 5.0 4.9 4.9 4.4 4.6 4.4 4.4 8.8 8.5 8.3 11.3 – 10.7 8.9 9.4 9.6
Std Deviation (1e-4) 1.2 1.3 1.1 1.4 1.3 1.1 1.3 1.6 1.2 0.9 1.0 0.8 1.4 1.2 1.2 2.7 – 2.5 1.5 1.5 1.7
Intensity Change (Log2) – – – 0.2 0.26 -0.13 -0.16 -0.16 -0.31 -0.23 -0.31 -0.32 0.69 0.65 0.61 1.06 – 0.97 0.71 0.79 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud -0.7 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Cytoplasm -0.5 0.3 -1.9 -5.5 -7.1 -8.2 -7.6 -9.7 -9.4 2.0 4.3 3.6 -4.9 – -4.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Mitochondria 3.6 -0.6 5.7 0 8.4 0 0 0 0 -3.5 -4.8 0 0 – 0
Nucleus 0 0 0 0 1.1 0 0.9 0.6 1.0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Vacuole 0 0 0 0.7 2.9 3.0 1.1 3.0 2.8 0 0 0 8.3 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7092 5.4591 4.688 4.1523 3.5537 4.6421 3.2137 4.3053 3.8186 3.3488 3.1898 4.0262 4.4246 6.9346 5.2257 5.2467 4.6491 5.2717
Actin 0.0392 0.0005 0.028 0.0475 0.0894 0.0163 0.0503 0.0011 0.034 0.0617 0.0536 0.0003 0.014 0.0005 0.0014 0.0009 0.0003 0.0006
Bud 0.0003 0.0003 0.0009 0.0002 0.0005 0.0001 0.0022 0.0001 0.0022 0.0013 0.0006 0.001 0.0002 0.0003 0.0002 0.0002 0.0001 0.0002
Bud Neck 0.0006 0.0002 0.0007 0.0008 0.0046 0.0012 0.0023 0.0001 0.0005 0.0016 0.0011 0.0008 0.0031 0.0005 0.0003 0.0004 0.0004 0.0012
Bud Periphery 0.0002 0.0001 0.0014 0.0001 0.0005 0.0001 0.0031 0.0001 0.0022 0.0021 0.0006 0.0004 0.0002 0.0001 0.0001 0 0 0
Bud Site 0.0008 0.0035 0.0025 0.0032 0.002 0.0001 0.0085 0.0004 0.0018 0.002 0.0027 0.0001 0.0008 0.0076 0.0003 0.0002 0.0003 0.0001
Cell Periphery 0.0001 0 0.0002 0 0.0001 0 0.0004 0.0001 0.0001 0.0004 0 0 0.0001 0.0001 0 0 0.0001 0
Cytoplasm 0.8364 0.97 0.8677 0.9144 0.7666 0.9285 0.7416 0.9698 0.8794 0.8196 0.8047 0.9354 0.8844 0.95 0.9319 0.9379 0.9281 0.938
Cytoplasmic Foci 0.0239 0.0021 0.0106 0.0044 0.0232 0.0071 0.0409 0.0024 0.0087 0.0232 0.0071 0.0063 0.0108 0.0045 0.0046 0.0031 0.0035 0.0051
Eisosomes 0.0001 0 0.0002 0.0001 0.0004 0.0001 0.0002 0 0.0001 0.0002 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0047 0.0012 0.0033 0.0011 0.0041 0.0023 0.0126 0.001 0.003 0.0081 0.0038 0.0031 0.008 0.0015 0.0028 0.0013 0.0059 0.0039
Endosome 0.0108 0.0012 0.0103 0.0009 0.0479 0.0068 0.041 0.0007 0.0081 0.0087 0.0382 0.0046 0.0047 0.0014 0.0027 0.0011 0.0019 0.0038
Golgi 0.0055 0.0001 0.0084 0.0006 0.0119 0.002 0.012 0.0001 0.004 0.0075 0.0141 0.0001 0.0013 0.0002 0.0003 0.0001 0.0001 0.0001
Lipid Particles 0.0077 0 0.0022 0.0003 0.0081 0.0024 0.0092 0 0.0007 0.0088 0.0021 0 0.0033 0 0.0002 0 0.0001 0.0001
Mitochondria 0.0014 0.0001 0.0108 0.0003 0.0021 0.0006 0.0113 0.0001 0.0085 0.0025 0.0069 0.0002 0.0004 0.0002 0.0002 0.0001 0.0001 0.0002
None 0.0419 0.0159 0.0356 0.0071 0.0054 0.0089 0.0231 0.0168 0.0212 0.0169 0.0416 0.008 0.0404 0.0221 0.0421 0.0446 0.0385 0.0225
Nuclear Periphery 0.0028 0.0007 0.0024 0.0013 0.0143 0.0041 0.0088 0.0007 0.0033 0.0029 0.0037 0.003 0.0032 0.001 0.0016 0.0014 0.004 0.002
Nucleolus 0.0001 0 0.0002 0 0.0004 0.0001 0.0004 0 0.0001 0.0001 0.0001 0 0.0002 0 0 0 0 0
Nucleus 0.0073 0.0033 0.0074 0.0145 0.0065 0.0141 0.0066 0.0055 0.0082 0.0077 0.0097 0.0343 0.0153 0.0087 0.0097 0.007 0.0144 0.0199
Peroxisomes 0.0122 0.0001 0.0048 0.0021 0.0031 0.001 0.0157 0 0.0117 0.0215 0.0052 0.0001 0.004 0.0002 0.0002 0.0001 0 0.0001
Punctate Nuclear 0.0027 0.0002 0.0008 0.0008 0.0062 0.0031 0.0034 0.0004 0.0009 0.002 0.001 0.0009 0.0044 0.0007 0.001 0.001 0.0009 0.0013
Vacuole 0.001 0.0005 0.0011 0.0003 0.0019 0.0009 0.0044 0.0004 0.0008 0.001 0.0016 0.0011 0.0009 0.0003 0.0004 0.0004 0.0011 0.0008
Vacuole Periphery 0.0002 0.0001 0.0006 0.0001 0.0009 0.0003 0.0022 0 0.0006 0.0003 0.0014 0.0003 0.0002 0 0.0001 0.0001 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.3332 38.1634 32.6756 40.1245 38.32 62.3604 51.944 39.0654 48.6266 55.1707
Translational Efficiency 2.5175 2.2792 1.8711 1.4606 2.1828 2.1046 1.56 2.246 1.7851 1.329

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
543 1442 342 78 1509 1338 1720 1424 2052 2780 2062 1502

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 732.81 890.80 1159.66 1432.69 857.13 937.61 1005.66 1244.66 824.23 913.33 1031.20 1254.42
Standard Deviation 125.18 151.20 149.91 413.96 117.78 132.47 143.65 415.63 131.74 144.40 155.63 417.63
Intensity Change Log 2 — 0.281662 0.662191 0.967215 — 0.129474 0.230557 0.538166 — 0.201617 0.445608 0.751833

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000131 0.000245 0.003193 0.001047 0.000097 0.000271 0.000285 0.000944 0.000106 0.000258 0.000767 0.000949
Bud Neck 0.004779 0.002582 0.003989 0.003053 0.002586 0.003246 0.007725 0.007806 0.003166 0.002902 0.007105 0.007559
Bud Site 0.005509 0.006270 0.007551 0.005063 0.002441 0.006483 0.007185 0.019750 0.003253 0.006372 0.007245 0.018987
Cell Periphery 0.000142 0.000077 0.000225 0.000283 0.000050 0.000060 0.000140 0.000168 0.000075 0.000069 0.000154 0.000174
Cytoplasm 0.614380* 0.755338* 0.309309* 0.258630* 0.703417* 0.723634* 0.430667* 0.453296* 0.679856* 0.740079* 0.410539* 0.443187*
Cytoplasmic Foci 0.151279 0.056303 0.008523 0.013329 0.094767 0.065241 0.008066 0.014531 0.109721 0.060605 0.008142 0.014469
Eisosomes 0.000075 0.000018 0.000044 0.000067 0.000026 0.000022 0.000030 0.000017 0.000039 0.000020 0.000032 0.000020
Endoplasmic Reticulum 0.001030 0.000955 0.007964 0.013368 0.001114 0.000876 0.002194 0.001584 0.001092 0.000917 0.003151 0.002196
Endosome 0.008170 0.004016 0.003093 0.003166 0.003248 0.004101 0.002550 0.010845 0.004551 0.004057 0.002640 0.010447
Golgi 0.000737 0.000857 0.000195 0.000136 0.000589 0.001563 0.000686 0.008272 0.000628 0.001197 0.000605 0.007849
Lipid Particles 0.002970 0.001552 0.001700 0.002385 0.001305 0.001244 0.001284 0.000792 0.001746 0.001403 0.001353 0.000874
Mitochondria 0.000695 0.001454 0.000493 0.000381 0.002704 0.001037 0.002042 0.003548 0.002172 0.001253 0.001785 0.003384
Mitotic Spindle 0.001006 0.003918 0.002150 0.017662 0.003899 0.008750 0.017154 0.055226 0.003133 0.006244 0.014665 0.053275
None 0.009116 0.006471 0.004592 0.005301 0.002124 0.003754 0.007271 0.016432 0.003974 0.005163 0.006827 0.015854
Nuclear Periphery 0.000403 0.000235 0.001543 0.001587 0.000233 0.000223 0.001262 0.001528 0.000278 0.000229 0.001309 0.001531
Nuclear Periphery Foci 0.001281 0.002255 0.004852 0.005988 0.000891 0.001323 0.000985 0.001523 0.000994 0.001807 0.001626 0.001755
Nucleolus 0.003907 0.000520 0.000305 0.000153 0.000562 0.000421 0.000542 0.000400 0.001447 0.000473 0.000503 0.000387
Nucleus 0.133980* 0.108361 0.546664* 0.360904* 0.124238 0.122124 0.455811* 0.351618* 0.126816 0.114985 0.470880* 0.352100*
Peroxisomes 0.001391 0.001039 0.001968 0.000219 0.000679 0.002128 0.000763 0.001492 0.000868 0.001563 0.000963 0.001426
Vacuole 0.058375 0.047028 0.091444 0.307144* 0.054541 0.052823 0.052061 0.046445 0.055555 0.049817 0.058593 0.059983
Vacuole Periphery 0.000644 0.000505 0.000204 0.000134 0.000489 0.000676 0.001300 0.003782 0.000530 0.000587 0.001118 0.003593

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.92 -2.27 -6.48 -5.64 1.34 -3.08 -9.25 -7.58 -4.15 -3.17 -4.70 -3.52 -8.19 -5.38 0.55
Bud Neck 3.39 -0.13 -0.51 -3.58 -0.46 -1.10 -5.09 -5.81 -3.71 0.38 0.62 -4.71 -5.05 -4.99 0.14
Bud Site -0.67 -1.56 -1.04 -0.26 0.90 -3.98 -4.68 -5.93 -1.97 -2.16 -4.41 -4.43 -5.31 -2.20 -1.97
Cell Periphery 4.10 -5.73 -4.20 -5.67 -1.64 -2.59 -10.79 -10.44 -9.51 -4.71 0.81 -9.84 -9.24 -9.54 -3.78
Cytoplasm -9.54 21.86 12.46 17.37 0.79 -1.43 41.75 36.06 35.80 -3.37 -6.90 45.05 36.00 43.94 -4.32
Cytoplasmic Foci 13.10 21.37 19.97 13.30 -0.59 6.46 26.23 25.44 20.33 -1.62 13.21 33.25 32.27 26.26 -1.51
Eisosomes 6.19 2.10 1.28 -6.07 -0.78 1.16 -4.25 0.73 -0.61 5.83 6.12 0.14 4.42 -3.28 6.88
Endoplasmic Reticulum 0.10 -10.63 -4.64 -4.66 1.18 0.73 -9.01 -3.85 -5.91 7.24 0.63 -13.49 -5.81 -8.39 9.06
Endosome 4.25 4.47 5.78 2.81 2.89 -2.03 3.72 0.86 2.07 -1.10 1.24 6.18 2.87 2.19 -0.62
Golgi -0.34 4.82 4.89 3.36 0.63 -2.56 1.40 -1.83 -0.24 -2.14 -2.57 2.21 -1.73 -0.78 -2.17
Lipid Particles 2.72 1.79 0.18 -2.93 -1.98 0.06 0.49 -0.62 -0.41 -1.00 1.24 1.80 1.49 -0.29 -0.66
Mitochondria -1.08 0.78 0.51 1.28 -0.40 1.68 1.12 1.18 -0.98 0.19 1.27 1.03 0.93 -0.50 -0.06
Mitotic Spindle -2.80 -1.75 -1.98 -1.77 -1.90 -2.09 -4.12 -7.89 -5.93 -4.69 -2.26 -4.50 -8.51 -7.43 -5.48
None 1.64 2.88 0.96 -0.19 -1.29 -3.52 -11.43 -15.25 -11.08 -7.41 -1.53 -3.53 -9.79 -9.81 -8.13
Nuclear Periphery 2.11 -14.87 -7.86 -8.76 -0.83 -0.33 -25.45 -17.70 -17.64 1.52 1.21 -27.88 -17.98 -18.56 1.38
Nuclear Periphery Foci -3.71 -7.62 -4.45 -2.88 1.69 -3.75 -2.19 -4.95 -0.59 -2.17 -6.39 -6.07 -5.13 1.74 1.82
Nucleolus 2.95 3.10 3.11 1.90 0.17 0.59 0.47 0.85 0.85 1.54 2.78 2.88 3.10 1.94 1.42
Nucleus 2.18 -23.73 -6.59 -7.33 5.00 0.14 -51.37 -35.11 -34.50 9.87 1.98 -55.82 -35.31 -37.37 11.09
Peroxisomes 1.39 -0.03 6.54 4.52 1.49 -2.95 1.40 1.90 3.51 0.60 -2.60 1.25 3.50 4.33 1.33
Vacuole -0.01 -7.59 -7.62 -7.66 -4.88 -0.88 -7.02 -12.82 -11.90 -6.39 -0.76 -9.61 -14.41 -14.18 -6.37
Vacuole Periphery 1.45 4.65 5.84 3.93 4.56 -0.85 -0.63 -1.08 -0.56 -0.40 -0.24 -0.30 -0.90 -0.80 -0.54
Standard name
Human Ortholog
Description Component of conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly of large protein complexes such as box C/D snoRNPs and RNA polymerase II; contains a single TPR domain with at least two TPR motifs; plays a role in determining prion variants
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tah1

Tah1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tah1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available