Standard name
Human Ortholog
Description Integral ER membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; SED4 has a paralog, SEC12, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.28 0.26 0.36 0.42 0.26 0.2 0.09 0.13 0.07 0.06 0.08 0 0.23 0.22 0.18 0.2 0.17 0.28 0.23 0.22 0.45 0.42 0.38 0.3
Bud 0 0.05 0.06 0.07 0.07 0.05 0.07 0.06 0.09 0.06 0.09 0.08 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.06 0.08 0.13 0.14 0.1 0.12 0.05 0.05 0 0 0.05 0 0.1 0.08 0.07 0.06 0 0.08 0.05 0.07 0.1 0.1 0.15 0.12
Cytoplasm 0.14 0.23 0.07 0.1 0.12 0.06 0.06 0.07 0.09 0.09 0.18 0.18 0.25 0.37 0.41 0.5 0.51 0.38 0 0.05 0 0 0 0
Endoplasmic Reticulum 0.72 0.55 0.56 0.31 0.36 0.52 0.28 0.25 0.16 0.18 0.13 0.1 0.58 0.46 0.4 0.08 0.07 0.05 0.55 0.45 0.29 0.25 0.29 0.37
Endosome 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0.06 0.07 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0.06
Mitochondria 0 0.18 0.22 0.41 0.31 0.56 0.69 0.71 0.74 0.78 0.73 0.69 0 0 0 0.11 0.12 0.08 0 0.06 0.05 0.1 0.06 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.1 0.16 0.21 0 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.07 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0.08 0 0 0 0 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 101 33 79 75 83 69 43 56 26 31 29 7 43 26 35 63 48 58 98 34 121 99 178 128
Bud 9 6 13 12 21 19 33 26 34 30 31 31 8 5 2 13 13 12 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 3 1 4 3 0 3 6 8
Bud Site 0 0 0 0 0 0 1 2 2 3 2 3 0 0 0 2 0 0 – – – – – –
Cell Periphery 21 10 28 25 32 43 25 21 15 11 17 17 19 10 14 20 10 17 20 10 27 23 71 49
Cytoplasm 49 30 16 18 37 21 28 28 34 44 66 73 46 44 77 160 144 78 8 8 0 2 4 2
Endoplasmic Reticulum 256 70 124 56 112 179 126 107 59 85 47 42 107 55 76 26 20 10 233 70 78 59 138 159
Endosome 3 1 8 7 18 2 12 20 8 2 4 14 1 0 5 18 20 12 9 3 3 4 6 10
Golgi 1 0 1 6 3 1 0 0 0 1 1 0 0 0 0 3 1 2 19 8 9 7 18 24
Mitochondria 14 23 49 74 99 193 314 301 273 371 267 279 1 0 1 35 35 17 11 10 13 22 25 28
Nucleus 0 0 0 0 0 0 1 4 5 3 2 2 0 0 1 2 5 3 1 0 0 0 0 0
Nuclear Periphery 1 1 0 0 1 3 1 1 5 8 1 3 0 1 0 0 0 0 0 0 1 1 1 0
Nucleolus 0 0 1 0 0 0 1 2 1 0 2 2 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 21 28 67 10 27 20 17 9 11 8 0 0 1 3 11 13 2 0 7 4 6 2
SpindlePole 5 0 1 0 0 0 2 1 2 2 1 3 2 0 0 45 19 20 2 0 0 0 0 0
Vac/Vac Membrane 3 1 0 0 3 2 5 14 23 23 30 33 1 1 3 8 11 14 7 4 0 3 6 6
Unique Cell Count 356 128 220 180 315 347 457 424 367 477 364 406 186 119 190 323 284 207 426 156 268 236 471 427
Labelled Cell Count 463 175 341 301 476 542 619 603 504 623 511 517 228 142 215 407 341 257 426 156 268 236 471 427


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 6.4 6.8 6.7 6.8 5.8 5.5 5.7 5.3 4.8 4.9 5.2 7.3 7.3 7.9 5.5 5.9 7.2 7.7 8.0 8.6
Std Deviation (1e-4) 0.8 0.9 1.5 1.4 1.9 1.0 1.6 1.7 1.2 1.3 1.0 1.3 0.7 0.8 1.1 1.2 1.2 1.6 1.1 1.2 1.6
Intensity Change (Log2) – – – -0.01 0.01 -0.23 -0.3 -0.24 -0.36 -0.49 -0.48 -0.4 0.1 0.1 0.22 -0.29 -0.2 0.09 0.18 0.24 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.2 -2.4 -4.2 -8.4 -6.7 -8.8 0 0 -11.9 -2.8 -2.7 -3.9 -4.3 -4.9 -1.7
Bud 0.3 0.4 -0.2 0.6 0.1 1.4 0 0 0.8 -0.7 -0.7 -2.6 -1.0 -0.7 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.3 -0.9 -0.1 -3.3 -3.5 -3.9 0 0 -3.9 -0.8 -1.2 -1.8 -2.6 -3.9 -1.5
Cytoplasm 1.0 1.7 -0.6 -0.6 -0.3 0.8 0.9 3.7 3.7 4.9 6.8 8.0 10.3 10.4 7.6
Endoplasmic Reticulum -5.1 -4.8 -1.1 -7.3 -7.8 -10.2 0 0 -12.5 0.2 -1.8 -3.3 -12.4 -12.2 -11.5
Endosome 0.1 1.1 0 -0.7 0.6 -1.0 0 0 -0.1 0 0 -0.6 1.0 1.7 1.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.1 2.3 7.8 11.3 11.8 12.3 0 0 11.1 -6.6 -5.6 -6.7 -3.6 -3.0 -4.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1.8 3.6 -3.4 -1.7 -2.4 -2.3 0 0 -4.3 -4.3 -3.5 -4.0 -4.8 -2.6 -1.2
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 5.5 3.6 4.4
Vacuole 0 0 0 0 0 3.8 3.3 4.4 4.3 0 0 0 0 0 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0131 3.413 3.1889 2.8017 2.4308 3.1346 4.2983 5.0197 4.6584 4.2806 4.146 4.8875 4.5112 4.6232 4.8115 4.6455 3.8605 4.624
Actin 0.3162 0.1951 0.2777 0.2646 0.325 0.2779 0.2519 0.2676 0.3976 0.2908 0.2733 0.4166 0.2814 0.2889 0.4325 0.3089 0.5911 0.3198
Bud 0.0007 0.0009 0.0017 0.0012 0.0018 0.0038 0.0003 0.0005 0.0011 0.0004 0.001 0.0008 0.0002 0.0004 0.0011 0.0029 0.0008 0.0009
Bud Neck 0.0054 0.0051 0.0084 0.0096 0.0079 0.03 0.0055 0.0053 0.0084 0.0043 0.0057 0.0451 0.0027 0.0051 0.0124 0.004 0.0044 0.0394
Bud Periphery 0.0012 0.0018 0.0078 0.011 0.0018 0.0033 0.0006 0.0006 0.0049 0.0006 0.0035 0.0022 0.0004 0.0005 0.0061 0.0093 0.0023 0.0033
Bud Site 0.0051 0.02 0.015 0.0093 0.0019 0.0041 0.008 0.0362 0.0118 0.0017 0.0025 0.0025 0.0023 0.0141 0.0091 0.0017 0.0011 0.0022
Cell Periphery 0.0065 0.02 0.0139 0.0378 0.0066 0.0133 0.0125 0.0167 0.0121 0.0084 0.0254 0.0082 0.0036 0.0108 0.0203 0.0067 0.0167 0.0156
Cytoplasm 0.05 0.174 0.0847 0.1411 0.0333 0.1114 0.1188 0.1983 0.0741 0.1405 0.0695 0.0938 0.0302 0.1503 0.0563 0.0784 0.0878 0.1298
Cytoplasmic Foci 0.0724 0.0784 0.0797 0.0876 0.0942 0.0758 0.1153 0.062 0.0661 0.0723 0.0896 0.0758 0.0577 0.0671 0.051 0.0679 0.0282 0.0476
Eisosomes 0.0074 0.007 0.0113 0.003 0.0055 0.0052 0.0054 0.0027 0.0057 0.0096 0.0055 0.0043 0.0035 0.0034 0.0176 0.003 0.0039 0.0074
Endoplasmic Reticulum 0.0815 0.1497 0.0935 0.0612 0.084 0.0923 0.0689 0.165 0.0586 0.0319 0.0784 0.0298 0.1263 0.1451 0.0951 0.1032 0.0932 0.0943
Endosome 0.0722 0.0521 0.0687 0.0953 0.0488 0.0604 0.0605 0.029 0.0459 0.0507 0.0618 0.0412 0.0678 0.0376 0.0274 0.0356 0.0127 0.0362
Golgi 0.2202 0.1631 0.2074 0.1745 0.1374 0.1959 0.07 0.1317 0.1718 0.2352 0.1921 0.1688 0.1559 0.1372 0.1732 0.2079 0.1068 0.1622
Lipid Particles 0.1076 0.09 0.0893 0.0596 0.1237 0.0968 0.2062 0.0681 0.1088 0.0491 0.1588 0.0692 0.2491 0.1233 0.067 0.086 0.0336 0.1107
Mitochondria 0.0139 0.0124 0.0094 0.0074 0.017 0.0109 0.0035 0.0011 0.0036 0.002 0.0066 0.0091 0.002 0.0007 0.0063 0.0229 0.0049 0.0079
None 0.018 0.0195 0.0112 0.0097 0.0721 0.0064 0.0159 0.0034 0.0214 0.0869 0.0167 0.0174 0.0015 0.0069 0.0047 0.0017 0.0019 0.0138
Nuclear Periphery 0.0025 0.002 0.0015 0.0015 0.0049 0.0014 0.0176 0.0026 0.001 0.002 0.0011 0.0008 0.0029 0.0024 0.0014 0.0016 0.0005 0.0012
Nucleolus 0.0002 0.0001 0.0001 0.0003 0.0011 0.0001 0.0023 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001
Nucleus 0.0004 0.0005 0.0003 0.0006 0.001 0.0005 0.0035 0.0003 0.0002 0.0009 0.0004 0.0003 0.0002 0.0002 0.0003 0.0003 0.0001 0.0004
Peroxisomes 0.0137 0.0053 0.0144 0.0169 0.0256 0.0062 0.0234 0.0056 0.0042 0.0028 0.0044 0.0103 0.0084 0.0029 0.0159 0.0547 0.0086 0.0032
Punctate Nuclear 0.0018 0.0008 0.001 0.0018 0.0033 0.0011 0.005 0.0007 0.0008 0.0065 0.0006 0.0015 0.0012 0.0012 0.0008 0.0006 0.0004 0.0007
Vacuole 0.002 0.0019 0.0021 0.0048 0.0019 0.0023 0.0039 0.0023 0.0015 0.0028 0.0022 0.0014 0.0021 0.0014 0.0011 0.0018 0.0007 0.0024
Vacuole Periphery 0.001 0.0005 0.0008 0.0014 0.0012 0.0009 0.001 0.0003 0.0004 0.0004 0.0006 0.0006 0.0005 0.0003 0.0003 0.0009 0.0002 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.6404 26.5254 36.1618 42.2966 25.9461 31.0355 39.0603 42.3072 39.0041 42.6119
Translational Efficiency 0.9498 0.9728 0.8689 0.7003 0.8604 1.0781 0.6789 0.6525 0.8831 0.7372

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Integral ER membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; SED4 has a paralog, SEC12, that arose from the whole genome duplication
Localization
Cell Percentages ER (45%)
Cell Cycle Regulation No
Subcompartmental Group ER-1

Sed4

Sed4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sed4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available