Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – – – – – – – – – – – – – – – – – 0 – – – 0.27 –
Bud – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Bud Neck – – – – – – – – – – – – – – – – – – 0.09 – – – 0 –
Bud Site – – – – – – – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Cytoplasm – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Endosome – – – – – – – – – – – – – – – – – – 0 – – – 0.06 –
Golgi – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Mitochondria – – – – – – – – – – – – – – – – – – 0 – – – 0.62 –
Nucleus – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Nucleolus – – – – – – – – – – – – – – – – – – 0.49 – – – 0 –
Peroxisomes – – – – – – – – – – – – – – – – – – 0 – – – 0 –
SpindlePole – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Vac/Vac Membrane – – – – – – – – – – – – – – – – – – 0 – – – 0 –
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Bud – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Bud Neck – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Bud Site – – – – – – – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Cytoplasm – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Endosome – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Golgi – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Mitochondria – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Nucleus – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Nucleolus – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Peroxisomes – – – – – – – – – – – – – – – – – – 0 – – – 0 –
SpindlePole – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Vac/Vac Membrane – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Unique Cell Count 1 1
Labelled Cell Count 1 1


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – – – – – – – – – – – – – – – – – – – –
Std Deviation (1e-4) – – – – – – – – – – – – – – – – – – – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –

−10123456−1−0.500.51−10123456−1−0.500.51−10123456−1−0.500.51−10123456−1−0.500.51
Multiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0583 9.3854 9.0362 6.4372 4.53 7.264 4.0457 11.8891 10.5974 9.7588 25.8047 8.2484 0.3523 0.5648 3.3962 1.1729 7.246 3.0269
Actin 0.0667 0.0134 0.0037 0.0479 0.0106 0.0211 0.0636 0.0014 0.0021 0.0359 0.0464 0.004 0.0018 0.0044 0.0175 0.0335 0.0007 0.001
Bud 0.0102 0.0165 0.0118 0.0009 0.0167 0.0015 0.0024 0.013 0.0101 0.0014 0.0086 0.0013 0.0019 0.0378 0.0022 0.0004 0.0001 0.0009
Bud Neck 0.0063 0.001 0.0003 0.0014 0.0017 0.0024 0.0013 0.0005 0.0004 0.001 0.0019 0.0015 0.0005 0.0009 0.0008 0.0025 0.0008 0.0008
Bud Periphery 0.0166 0.0076 0.0063 0.0013 0.0263 0.002 0.0017 0.0053 0.0045 0.0013 0.0058 0.0011 0.0012 0.0123 0.0018 0.0006 0 0.001
Bud Site 0.034 0.053 0.0032 0.003 0.0098 0.0003 0.0055 0.0049 0.0029 0.0043 0.0025 0.0003 0.0009 0.0154 0.0147 0.0045 0.0018 0.0001
Cell Periphery 0.0027 0.0015 0.0007 0.0004 0.005 0.0003 0.0007 0.0005 0.0001 0.0002 0.0007 0.0002 0.0005 0.0004 0.0005 0.0001 0 0.0001
Cytoplasm 0.322 0.5369 0.6112 0.5514 0.5759 0.6677 0.4152 0.6552 0.7106 0.6512 0.7611 0.6831 0.3296 0.371 0.6406 0.6125 0.4958 0.5177
Cytoplasmic Foci 0.0206 0.0044 0.0055 0.0123 0.0076 0.0179 0.0473 0.0073 0.0073 0.0178 0.0144 0.0137 0.0866 0.0563 0.0044 0.0311 0.0711 0.0114
Eisosomes 0.0015 0.0007 0.0008 0.001 0.0011 0.0001 0.001 0.0002 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0
Endoplasmic Reticulum 0.0119 0.0025 0.0053 0.0028 0.0074 0.0045 0.0091 0.0013 0.0017 0.0018 0.0018 0.0038 0.0044 0.0018 0.0022 0.0005 0.0013 0.0019
Endosome 0.0357 0.0023 0.0079 0.0127 0.0164 0.0047 0.0335 0.0028 0.0112 0.051 0.0078 0.0088 0.0042 0.0008 0.0039 0.0026 0.0006 0.0008
Golgi 0.0179 0.0005 0.0007 0.0263 0.0087 0.0025 0.013 0.0026 0.0137 0.0148 0.0078 0.0027 0.0019 0.0008 0.0061 0.0067 0.0008 0.0001
Lipid Particles 0.0149 0.0015 0.0007 0.0047 0.0029 0.0009 0.0242 0.0006 0.0075 0.0322 0.0197 0.0032 0.0052 0.0002 0.0021 0.0168 0.0056 0.0003
Mitochondria 0.0273 0.0066 0.0387 0.0524 0.0547 0.0027 0.0423 0.0006 0.0172 0.013 0.0029 0.0125 0.0122 0.0004 0.0243 0.0089 0.0015 0.0001
None 0.3732 0.3374 0.2916 0.2688 0.2191 0.2592 0.2477 0.2992 0.2038 0.1366 0.0911 0.2516 0.4828 0.4896 0.2734 0.2333 0.3507 0.4595
Nuclear Periphery 0.0062 0.0007 0.0018 0.0009 0.0027 0.0013 0.0149 0.0004 0.001 0.0012 0.0024 0.0045 0.0016 0.0005 0.0005 0.0002 0.0004 0.0006
Nucleolus 0.0011 0.0004 0.0003 0.0001 0.0004 0.0002 0.0027 0.0004 0.0001 0.0029 0.0003 0.0002 0.003 0.0001 0.0001 0.0001 0.0003 0
Nucleus 0.0029 0.004 0.0034 0.0027 0.0065 0.0042 0.0213 0.0013 0.0014 0.0023 0.0033 0.0028 0.0072 0.0023 0.0013 0.0008 0.0026 0.0016
Peroxisomes 0.0112 0.0036 0.0007 0.0061 0.0025 0.001 0.0248 0.0003 0.0025 0.0144 0.0142 0.0004 0.0434 0.0041 0.0021 0.0427 0.0639 0.0007
Punctate Nuclear 0.0055 0.0009 0.0006 0.0004 0.0003 0.0024 0.0162 0.0012 0.0007 0.0042 0.0052 0.0017 0.0032 0.0004 0.0005 0.0014 0.0016 0.0004
Vacuole 0.0084 0.0044 0.0041 0.0018 0.0202 0.0027 0.0085 0.0009 0.0007 0.0092 0.0016 0.0015 0.0057 0.0004 0.0006 0.0002 0.0002 0.0007
Vacuole Periphery 0.0032 0.0002 0.0007 0.0007 0.0036 0.0006 0.0029 0.0001 0.0004 0.0032 0.0004 0.0011 0.0022 0.0001 0.0002 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.5904 6.4469 12.9848 14.7169 8.1816 12.0988 13.5025 14.0627 12.4258 14.1179
Translational Efficiency 0.3418 0.5559 0.3322 0.2742 0.3659 0.2906 0.3349 0.2288 0.3045 0.2529

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1821 749 186 964 761 1229 1291 10 2582 1978 1477 974

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 776.62 1134.43 1074.00 768.40 962.52 981.43 906.85 825.40 831.41 1039.37 927.90 768.99
Standard Deviation 317.86 811.19 329.51 330.01 759.91 828.44 267.98 168.20 498.64 825.29 281.99 328.80
Intensity Change Log 2 — 0.546687 0.467713 -0.015351 — 0.028069 -0.085953 -0.221723 — 0.282870 0.187746 -0.125903

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000174 0.001163 0.000663 0.006520 0.000445 0.001759 0.000300 0.000241 0.000254 0.001533 0.000346 0.006455
Bud Neck 0.013448 0.007797 0.004052 0.012741 0.006874 0.015676 0.004290 0.002010 0.011511 0.012693 0.004260 0.012631
Bud Site 0.021582 0.020302 0.004340 0.151790 0.014287 0.024768 0.009450 0.001888 0.019432 0.023077 0.008807 0.150251
Cell Periphery 0.000428 0.000287 0.000269 0.018363 0.000664 0.001182 0.000140 0.000234 0.000497 0.000843 0.000157 0.018177
Cytoplasm 0.706205 0.672349 0.273465 0.681348 0.691372 0.654508 0.545330 0.267465 0.701833 0.661264 0.511093 0.677099
Cytoplasmic Foci 0.061778 0.091031 0.018592 0.034532 0.088208 0.075324 0.019260 0.005515 0.069568 0.081272 0.019176 0.034234
Eisosomes 0.000072 0.000060 0.000095 0.000373 0.000075 0.001460 0.000035 0.000039 0.000073 0.000930 0.000043 0.000370
Endoplasmic Reticulum 0.000480 0.001202 0.007110 0.004337 0.000814 0.001634 0.001310 0.005811 0.000578 0.001470 0.002040 0.004352
Endosome 0.003425 0.009194 0.005787 0.007548 0.005919 0.009350 0.003734 0.049272 0.004160 0.009291 0.003993 0.007977
Golgi 0.000617 0.002311 0.000182 0.003057 0.001082 0.002947 0.000617 0.007752 0.000754 0.002706 0.000562 0.003106
Lipid Particles 0.001798 0.003469 0.003691 0.003648 0.002721 0.004405 0.001327 0.001519 0.002070 0.004051 0.001624 0.003626
Mitochondria 0.003992 0.007613 0.000619 0.000263 0.003322 0.005978 0.001130 0.030892 0.003794 0.006597 0.001066 0.000578
Mitotic Spindle 0.002831 0.009635 0.001436 0.000728 0.002606 0.018566 0.019227 0.003467 0.002765 0.015184 0.016987 0.000756
None 0.026015 0.010522 0.006345 0.010407 0.032601 0.058011 0.007770 0.006947 0.027956 0.040029 0.007591 0.010372
Nuclear Periphery 0.000232 0.000289 0.001794 0.000287 0.000295 0.000252 0.001171 0.002044 0.000251 0.000266 0.001249 0.000305
Nuclear Periphery Foci 0.000694 0.002326 0.003631 0.005309 0.003708 0.002666 0.001134 0.001612 0.001583 0.002537 0.001448 0.005271
Nucleolus 0.001116 0.001152 0.000809 0.000205 0.001432 0.002151 0.000734 0.000366 0.001209 0.001773 0.000743 0.000206
Nucleus 0.117206 0.098394 0.534399 0.014653 0.104303 0.073548 0.314026 0.500571 0.113403 0.082956 0.341778 0.019642
Peroxisomes 0.001048 0.005240 0.000438 0.000648 0.001820 0.006475 0.000910 0.001260 0.001276 0.006007 0.000851 0.000654
Vacuole 0.036025 0.053243 0.131993 0.042267 0.036423 0.037725 0.066918 0.075018 0.036143 0.043601 0.075113 0.042603
Vacuole Periphery 0.000833 0.002421 0.000290 0.000975 0.001029 0.001614 0.001186 0.036078 0.000891 0.001919 0.001073 0.001336

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.82 -7.66 -5.95 -4.64 -5.23 NaN NaN NaN NaN NaN -2.45 -2.59 -5.87 -4.37 -5.72
Bud Neck 4.39 6.92 0.32 -2.98 -4.61 NaN NaN NaN NaN NaN -0.44 7.10 -0.71 -0.36 -4.84
Bud Site 0.62 8.26 -19.05 -18.97 -22.17 NaN NaN NaN NaN NaN -1.45 6.91 -19.60 -19.15 -21.61
Cell Periphery 2.45 1.75 -9.90 -9.96 -9.94 NaN NaN NaN NaN NaN -2.87 8.77 -9.85 -9.67 -10.03
Cytoplasm 3.24 27.50 1.93 -1.25 -23.93 NaN NaN NaN NaN NaN 4.77 28.43 1.94 -1.67 -19.59
Cytoplasmic Foci -4.02 14.26 9.08 9.12 -5.85 NaN NaN NaN NaN NaN -2.42 20.99 11.85 12.15 -7.42
Eisosomes 1.08 -2.89 -5.15 -5.28 -4.63 NaN NaN NaN NaN NaN -3.56 3.68 -5.14 2.11 -5.47
Endoplasmic Reticulum -2.28 -8.83 -3.78 -3.08 2.87 NaN NaN NaN NaN NaN -3.40 -12.74 -3.73 -2.89 -1.44
Endosome -3.98 -2.98 -4.66 0.48 -2.10 NaN NaN NaN NaN NaN -5.77 1.86 -3.97 0.77 -4.69
Golgi -2.99 2.66 -4.42 -1.38 -5.21 NaN NaN NaN NaN NaN -5.30 2.24 -4.26 -0.94 -4.92
Lipid Particles -1.31 -4.22 -3.84 -0.44 0.10 NaN NaN NaN NaN NaN -2.77 1.28 -3.36 0.14 -4.03
Mitochondria -1.27 3.73 4.07 3.54 3.51 NaN NaN NaN NaN NaN -1.73 3.83 4.35 5.29 1.30
Mitotic Spindle -2.72 0.89 2.31 3.72 1.53 NaN NaN NaN NaN NaN -5.71 -5.21 2.42 6.62 6.05
None 7.93 10.90 5.96 -0.45 -2.90 NaN NaN NaN NaN NaN -2.65 12.57 6.96 7.12 -2.14
Nuclear Periphery -0.78 -14.60 -0.41 -0.11 8.68 NaN NaN NaN NaN NaN -0.42 -15.80 -0.44 -0.34 6.92
Nuclear Periphery Foci -6.07 -6.10 -11.58 -4.73 -0.73 NaN NaN NaN NaN NaN -3.86 -1.56 -8.48 -5.25 -7.79
Nucleolus 0.22 3.77 8.42 2.95 4.78 NaN NaN NaN NaN NaN -2.05 4.23 9.63 5.87 4.12
Nucleus 1.74 -18.10 27.02 17.01 23.24 NaN NaN NaN NaN NaN 6.30 -28.93 25.00 17.45 41.45
Peroxisomes -3.41 4.36 2.53 3.81 -3.67 NaN NaN NaN NaN NaN -4.59 2.61 4.00 5.28 -0.29
Vacuole -4.68 -9.39 -0.91 4.13 9.22 NaN NaN NaN NaN NaN -3.61 -19.24 -1.34 2.14 18.13
Vacuole Periphery -1.71 2.72 -0.64 1.48 -4.01 NaN NaN NaN NaN NaN -2.14 0.79 -1.02 1.19 -1.47
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
Localization
Cell Percentages cytoplasm (73%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Srb8

Srb8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srb8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available