Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – – – – – – – – – – – – – – – – – 0 – – – 0.27 –
Bud – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Bud Neck – – – – – – – – – – – – – – – – – – 0.09 – – – 0 –
Bud Site – – – – – – – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Cytoplasm – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Endosome – – – – – – – – – – – – – – – – – – 0 – – – 0.06 –
Golgi – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Mitochondria – – – – – – – – – – – – – – – – – – 0 – – – 0.62 –
Nucleus – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Nucleolus – – – – – – – – – – – – – – – – – – 0.49 – – – 0 –
Peroxisomes – – – – – – – – – – – – – – – – – – 0 – – – 0 –
SpindlePole – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Vac/Vac Membrane – – – – – – – – – – – – – – – – – – 0 – – – 0 –
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Bud – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Bud Neck – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Bud Site – – – – – – – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Cytoplasm – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Endosome – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Golgi – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Mitochondria – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Nucleus – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Nucleolus – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Peroxisomes – – – – – – – – – – – – – – – – – – 0 – – – 0 –
SpindlePole – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Vac/Vac Membrane – – – – – – – – – – – – – – – – – – 0 – – – 0 –
Unique Cell Count 1 1
Labelled Cell Count 1 1


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – – – – – – – – – – – – – – – – – – – –
Std Deviation (1e-4) – – – – – – – – – – – – – – – – – – – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0583 9.3854 9.0362 6.4372 4.53 7.264 4.0457 11.8891 10.5974 9.7588 25.8047 8.2484 0.3523 0.5648 3.3962 1.1729 7.246 3.0269
Actin 0.0667 0.0134 0.0037 0.0479 0.0106 0.0211 0.0636 0.0014 0.0021 0.0359 0.0464 0.004 0.0018 0.0044 0.0175 0.0335 0.0007 0.001
Bud 0.0102 0.0165 0.0118 0.0009 0.0167 0.0015 0.0024 0.013 0.0101 0.0014 0.0086 0.0013 0.0019 0.0378 0.0022 0.0004 0.0001 0.0009
Bud Neck 0.0063 0.001 0.0003 0.0014 0.0017 0.0024 0.0013 0.0005 0.0004 0.001 0.0019 0.0015 0.0005 0.0009 0.0008 0.0025 0.0008 0.0008
Bud Periphery 0.0166 0.0076 0.0063 0.0013 0.0263 0.002 0.0017 0.0053 0.0045 0.0013 0.0058 0.0011 0.0012 0.0123 0.0018 0.0006 0 0.001
Bud Site 0.034 0.053 0.0032 0.003 0.0098 0.0003 0.0055 0.0049 0.0029 0.0043 0.0025 0.0003 0.0009 0.0154 0.0147 0.0045 0.0018 0.0001
Cell Periphery 0.0027 0.0015 0.0007 0.0004 0.005 0.0003 0.0007 0.0005 0.0001 0.0002 0.0007 0.0002 0.0005 0.0004 0.0005 0.0001 0 0.0001
Cytoplasm 0.322 0.5369 0.6112 0.5514 0.5759 0.6677 0.4152 0.6552 0.7106 0.6512 0.7611 0.6831 0.3296 0.371 0.6406 0.6125 0.4958 0.5177
Cytoplasmic Foci 0.0206 0.0044 0.0055 0.0123 0.0076 0.0179 0.0473 0.0073 0.0073 0.0178 0.0144 0.0137 0.0866 0.0563 0.0044 0.0311 0.0711 0.0114
Eisosomes 0.0015 0.0007 0.0008 0.001 0.0011 0.0001 0.001 0.0002 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0
Endoplasmic Reticulum 0.0119 0.0025 0.0053 0.0028 0.0074 0.0045 0.0091 0.0013 0.0017 0.0018 0.0018 0.0038 0.0044 0.0018 0.0022 0.0005 0.0013 0.0019
Endosome 0.0357 0.0023 0.0079 0.0127 0.0164 0.0047 0.0335 0.0028 0.0112 0.051 0.0078 0.0088 0.0042 0.0008 0.0039 0.0026 0.0006 0.0008
Golgi 0.0179 0.0005 0.0007 0.0263 0.0087 0.0025 0.013 0.0026 0.0137 0.0148 0.0078 0.0027 0.0019 0.0008 0.0061 0.0067 0.0008 0.0001
Lipid Particles 0.0149 0.0015 0.0007 0.0047 0.0029 0.0009 0.0242 0.0006 0.0075 0.0322 0.0197 0.0032 0.0052 0.0002 0.0021 0.0168 0.0056 0.0003
Mitochondria 0.0273 0.0066 0.0387 0.0524 0.0547 0.0027 0.0423 0.0006 0.0172 0.013 0.0029 0.0125 0.0122 0.0004 0.0243 0.0089 0.0015 0.0001
None 0.3732 0.3374 0.2916 0.2688 0.2191 0.2592 0.2477 0.2992 0.2038 0.1366 0.0911 0.2516 0.4828 0.4896 0.2734 0.2333 0.3507 0.4595
Nuclear Periphery 0.0062 0.0007 0.0018 0.0009 0.0027 0.0013 0.0149 0.0004 0.001 0.0012 0.0024 0.0045 0.0016 0.0005 0.0005 0.0002 0.0004 0.0006
Nucleolus 0.0011 0.0004 0.0003 0.0001 0.0004 0.0002 0.0027 0.0004 0.0001 0.0029 0.0003 0.0002 0.003 0.0001 0.0001 0.0001 0.0003 0
Nucleus 0.0029 0.004 0.0034 0.0027 0.0065 0.0042 0.0213 0.0013 0.0014 0.0023 0.0033 0.0028 0.0072 0.0023 0.0013 0.0008 0.0026 0.0016
Peroxisomes 0.0112 0.0036 0.0007 0.0061 0.0025 0.001 0.0248 0.0003 0.0025 0.0144 0.0142 0.0004 0.0434 0.0041 0.0021 0.0427 0.0639 0.0007
Punctate Nuclear 0.0055 0.0009 0.0006 0.0004 0.0003 0.0024 0.0162 0.0012 0.0007 0.0042 0.0052 0.0017 0.0032 0.0004 0.0005 0.0014 0.0016 0.0004
Vacuole 0.0084 0.0044 0.0041 0.0018 0.0202 0.0027 0.0085 0.0009 0.0007 0.0092 0.0016 0.0015 0.0057 0.0004 0.0006 0.0002 0.0002 0.0007
Vacuole Periphery 0.0032 0.0002 0.0007 0.0007 0.0036 0.0006 0.0029 0.0001 0.0004 0.0032 0.0004 0.0011 0.0022 0.0001 0.0002 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.5904 6.4469 12.9848 14.7169 8.1816 12.0988 13.5025 14.0627 12.4258 14.1179
Translational Efficiency 0.3418 0.5559 0.3322 0.2742 0.3659 0.2906 0.3349 0.2288 0.3045 0.2529

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1821 749 186 964 761 1229 1291 10 2582 1978 1477 974

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 776.62 1134.43 1074.00 768.40 962.52 981.43 906.85 825.40 831.41 1039.37 927.90 768.99
Standard Deviation 317.86 811.19 329.51 330.01 759.91 828.44 267.98 168.20 498.64 825.29 281.99 328.80
Intensity Change Log 2 — 0.546687 0.467713 -0.015351 — 0.028069 -0.085953 -0.221723 — 0.282870 0.187746 -0.125903

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000174 0.001163 0.000663 0.006520 0.000445 0.001759 0.000300 0.000241 0.000254 0.001533 0.000346 0.006455
Bud Neck 0.013448 0.007797 0.004052 0.012741 0.006874 0.015676 0.004290 0.002010 0.011511 0.012693 0.004260 0.012631
Bud Site 0.021582 0.020302 0.004340 0.151790* 0.014287 0.024768 0.009450 0.001888 0.019432 0.023077 0.008807 0.150251*
Cell Periphery 0.000428 0.000287 0.000269 0.018363 0.000664 0.001182 0.000140 0.000234 0.000497 0.000843 0.000157 0.018177
Cytoplasm 0.706205* 0.672349* 0.273465* 0.681348* 0.691372* 0.654508* 0.545330* 0.267465* 0.701833* 0.661264* 0.511093* 0.677099*
Cytoplasmic Foci 0.061778 0.091031 0.018592 0.034532 0.088208 0.075324 0.019260 0.005515 0.069568 0.081272 0.019176 0.034234
Eisosomes 0.000072 0.000060 0.000095 0.000373 0.000075 0.001460 0.000035 0.000039 0.000073 0.000930 0.000043 0.000370
Endoplasmic Reticulum 0.000480 0.001202 0.007110 0.004337 0.000814 0.001634 0.001310 0.005811 0.000578 0.001470 0.002040 0.004352
Endosome 0.003425 0.009194 0.005787 0.007548 0.005919 0.009350 0.003734 0.049272 0.004160 0.009291 0.003993 0.007977
Golgi 0.000617 0.002311 0.000182 0.003057 0.001082 0.002947 0.000617 0.007752 0.000754 0.002706 0.000562 0.003106
Lipid Particles 0.001798 0.003469 0.003691 0.003648 0.002721 0.004405 0.001327 0.001519 0.002070 0.004051 0.001624 0.003626
Mitochondria 0.003992 0.007613 0.000619 0.000263 0.003322 0.005978 0.001130 0.030892 0.003794 0.006597 0.001066 0.000578
Mitotic Spindle 0.002831 0.009635 0.001436 0.000728 0.002606 0.018566 0.019227 0.003467 0.002765 0.015184 0.016987 0.000756
None 0.026015 0.010522 0.006345 0.010407 0.032601 0.058011 0.007770 0.006947 0.027956 0.040029 0.007591 0.010372
Nuclear Periphery 0.000232 0.000289 0.001794 0.000287 0.000295 0.000252 0.001171 0.002044 0.000251 0.000266 0.001249 0.000305
Nuclear Periphery Foci 0.000694 0.002326 0.003631 0.005309 0.003708 0.002666 0.001134 0.001612 0.001583 0.002537 0.001448 0.005271
Nucleolus 0.001116 0.001152 0.000809 0.000205 0.001432 0.002151 0.000734 0.000366 0.001209 0.001773 0.000743 0.000206
Nucleus 0.117206 0.098394 0.534399* 0.014653 0.104303 0.073548 0.314026* 0.500571* 0.113403 0.082956 0.341778* 0.019642
Peroxisomes 0.001048 0.005240 0.000438 0.000648 0.001820 0.006475 0.000910 0.001260 0.001276 0.006007 0.000851 0.000654
Vacuole 0.036025 0.053243 0.131993 0.042267 0.036423 0.037725 0.066918 0.075018 0.036143 0.043601 0.075113 0.042603
Vacuole Periphery 0.000833 0.002421 0.000290 0.000975 0.001029 0.001614 0.001186 0.036078 0.000891 0.001919 0.001073 0.001336

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.82 -7.66 -5.95 -4.64 -5.23 NaN NaN NaN NaN NaN -2.45 -2.59 -5.87 -4.37 -5.72
Bud Neck 4.39 6.92 0.32 -2.98 -4.61 NaN NaN NaN NaN NaN -0.44 7.10 -0.71 -0.36 -4.84
Bud Site 0.62 8.26 -19.05 -18.97 -22.17* NaN NaN NaN NaN NaN -1.45 6.91 -19.60 -19.15 -21.61
Cell Periphery 2.45 1.75 -9.90 -9.96 -9.94 NaN NaN NaN NaN NaN -2.87 8.77 -9.85 -9.67 -10.03
Cytoplasm 3.24 27.50 1.93 -1.25 -23.93 NaN NaN NaN NaN NaN 4.77 28.43 1.94 -1.67 -19.59
Cytoplasmic Foci -4.02 14.26 9.08 9.12 -5.85 NaN NaN NaN NaN NaN -2.42 20.99 11.85 12.15 -7.42
Eisosomes 1.08 -2.89 -5.15 -5.28 -4.63 NaN NaN NaN NaN NaN -3.56 3.68 -5.14 2.11 -5.47
Endoplasmic Reticulum -2.28 -8.83 -3.78 -3.08 2.87 NaN NaN NaN NaN NaN -3.40 -12.74 -3.73 -2.89 -1.44
Endosome -3.98 -2.98 -4.66 0.48 -2.10 NaN NaN NaN NaN NaN -5.77 1.86 -3.97 0.77 -4.69
Golgi -2.99 2.66 -4.42 -1.38 -5.21 NaN NaN NaN NaN NaN -5.30 2.24 -4.26 -0.94 -4.92
Lipid Particles -1.31 -4.22 -3.84 -0.44 0.10 NaN NaN NaN NaN NaN -2.77 1.28 -3.36 0.14 -4.03
Mitochondria -1.27 3.73 4.07 3.54 3.51 NaN NaN NaN NaN NaN -1.73 3.83 4.35 5.29 1.30
Mitotic Spindle -2.72 0.89 2.31 3.72 1.53 NaN NaN NaN NaN NaN -5.71 -5.21 2.42 6.62 6.05
None 7.93 10.90 5.96 -0.45 -2.90 NaN NaN NaN NaN NaN -2.65 12.57 6.96 7.12 -2.14
Nuclear Periphery -0.78 -14.60 -0.41 -0.11 8.68 NaN NaN NaN NaN NaN -0.42 -15.80 -0.44 -0.34 6.92
Nuclear Periphery Foci -6.07 -6.10 -11.58 -4.73 -0.73 NaN NaN NaN NaN NaN -3.86 -1.56 -8.48 -5.25 -7.79
Nucleolus 0.22 3.77 8.42 2.95 4.78 NaN NaN NaN NaN NaN -2.05 4.23 9.63 5.87 4.12
Nucleus 1.74 -18.10 27.02 17.01 23.24 NaN NaN NaN NaN NaN 6.30 -28.93 25.00 17.45 41.45
Peroxisomes -3.41 4.36 2.53 3.81 -3.67 NaN NaN NaN NaN NaN -4.59 2.61 4.00 5.28 -0.29
Vacuole -4.68 -9.39 -0.91 4.13 9.22 NaN NaN NaN NaN NaN -3.61 -19.24 -1.34 2.14 18.13
Vacuole Periphery -1.71 2.72 -0.64 1.48 -4.01 NaN NaN NaN NaN NaN -2.14 0.79 -1.02 1.19 -1.47
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
Localization
Cell Percentages cytoplasm (73%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Srb8

Srb8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srb8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available