Standard name
Human Ortholog
Description Component of the ADA histone acetyltransferase complex; Ahc2p and Ahc1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ahc1p to the complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05
Bud Neck 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0.07 0 0.11 – 0.2 0.16 0.17 0.12 0.17 0.12 0.09 0.19 0.17 0.17 0.15 0 0 0.06 0.13 0.14 0.18
Endoplasmic Reticulum 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 – 0.61 0.19 0.64 – 0.63 0.78 0.7 0.75 0.73 0.1 0.05 0 0 0.1 0 0 0 0 0 0 0
Nucleus 0.84 0.89 – 0.58 0.86 0.54 – 0.38 0.36 0.35 0.33 0.32 0.77 0.76 0.75 0.57 0.56 0.66 0.77 0.8 0.66 0.58 0.43 0.35
Nuclear Periphery 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0.05 0
Peroxisomes 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05
Vac/Vac Membrane 0.07 0.14 – 0 0 0 – 0.07 0 0 0 0.05 0 0.07 0 0.24 0.23 0.13 0.08 0.07 0.12 0.16 0.14 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 – 0 0 1 – 10 5 5 11 8 0 1 0 0 0 0 5 1 0 1 11 10
Bud Neck 0 0 – 0 0 0 – 1 0 1 0 1 0 0 0 0 0 0 1 0 0 0 3 1
Bud Site 0 0 – 1 0 3 – 12 3 4 11 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 – 0 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 10 0 – 8 5 21 – 82 32 53 42 56 15 15 30 13 15 20 5 1 2 6 24 35
Endoplasmic Reticulum 1 0 – 0 0 0 – 1 0 2 1 1 0 1 0 2 2 4 0 0 0 0 0 1
Endosome 1 0 – 0 3 0 – 3 0 2 0 0 1 4 1 3 2 8 5 1 0 0 4 6
Golgi 2 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 2 0 0 0 1 0
Mitochondria 34 0 – 68 23 118 – 255 152 214 254 238 12 9 4 3 9 5 5 0 0 0 1 6
Nucleus 268 39 – 65 106 98 – 156 70 108 113 104 94 126 120 43 49 87 274 62 28 28 73 67
Nuclear Periphery 0 0 – 0 0 1 – 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3 0 – 3 3 1 – 12 0 3 15 10 4 10 4 2 1 4 13 2 0 1 8 7
Peroxisomes 1 0 – 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 2 2 – 8 2 8 6 2 1 0 0 1 0 0 9 0 1 1 13 10
Vac/Vac Membrane 23 6 – 4 4 5 – 29 7 11 8 15 5 12 5 18 20 17 29 5 5 7 24 41
Unique Cell Count 318 44 112 123 183 406 196 306 338 326 122 166 159 75 87 132 356 78 44 49 171 194
Labelled Cell Count 344 45 149 146 250 570 271 412 464 448 132 178 165 85 98 147 356 78 44 49 171 194


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 6.3 – 4.1 4.8 4.1 – 5.1 3.6 4.4 4.4 4.0 5.8 5.8 5.6 7.9 8.1 7.8 – 5.5 5.6
Std Deviation (1e-4) 0.7 0.8 – 1.0 1.4 2.3 – 4.2 2.8 4.0 4.3 3.6 1.6 1.4 1.5 1.6 1.8 1.9 – 1.8 1.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0324 1.4194 0.8917 1.0052 1.1953 1.023 0.9885 0.8448 0.6438 0.2783 0.5329 1.0213 2.4536 2.2187 2.2187 2.3878 2.3194 2.1301
Actin 0.0084 0.0001 0.0075 0.0023 0.0739 0.0036 0.0153 0.0006 0.0037 0.0094 0.0013 0.0175 0.0501 0.0002 0.0118 0.0028 0.0419 0.0013
Bud 0.0004 0.0001 0.0003 0.0008 0.001 0.0001 0.0006 0.0003 0.0005 0.0006 0.0003 0.0001 0.0013 0.0001 0.0005 0.0001 0.0008 0.0002
Bud Neck 0.0017 0.0003 0.0017 0.0006 0.0012 0.0031 0.001 0.0008 0.003 0.0005 0.0013 0.0028 0.0028 0.0003 0.0009 0.0002 0.0014 0.004
Bud Periphery 0.0005 0 0.0002 0.0055 0.0007 0.0002 0.0009 0.0003 0.0006 0.001 0.0003 0.0002 0.0018 0 0.0005 0.0001 0.0011 0.0003
Bud Site 0.0029 0.0005 0.003 0.0008 0.0125 0.0001 0.0015 0.0021 0.0026 0.001 0.0006 0.0002 0.0035 0.0009 0.0086 0.0002 0.0022 0.0011
Cell Periphery 0.0002 0 0.0001 0.0007 0.0002 0 0.0002 0.0001 0.0002 0 0.0001 0 0.0003 0 0.0002 0 0.0002 0.0001
Cytoplasm 0.0259 0.0442 0.0214 0.0082 0.0041 0.0013 0.0219 0.0411 0.023 0.0009 0.0016 0.0031 0.0109 0.0514 0.0307 0.0134 0.0117 0.0086
Cytoplasmic Foci 0.0329 0.0004 0.005 0.0033 0.1215 0.0008 0.0121 0.0005 0.0037 0.0005 0.0049 0.0029 0.0154 0.0003 0.0105 0.0062 0.0125 0.0005
Eisosomes 0.0001 0 0.0001 0.0001 0.0005 0 0.0001 0 0.0001 0.0001 0 0 0.0005 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0033 0.001 0.0004 0.0004 0.0007 0.0002 0.0044 0.0013 0.0008 0.0001 0.0005 0.001 0.0037 0.0008 0.0012 0.0031 0.0029 0.0004
Endosome 0.0271 0.0006 0.0036 0.003 0.0153 0.0016 0.0566 0.0009 0.0183 0.0006 0.0383 0.0084 0.0252 0.0004 0.0044 0.0145 0.036 0.0037
Golgi 0.0135 0 0.0026 0.0033 0.0229 0.0016 0.0105 0 0.0157 0.0011 0.015 0.0019 0.0108 0 0.0022 0.0046 0.0256 0.0006
Lipid Particles 0.0131 0.0001 0.0026 0.0131 0.0722 0.0101 0.0146 0 0.005 0.0003 0.0085 0.0026 0.0317 0 0.0035 0.0111 0.026 0.0005
Mitochondria 0.014 0.0002 0.013 0.0245 0.0167 0.001 0.007 0.0005 0.015 0.0423 0.0058 0.0005 0.007 0.0002 0.0015 0.0069 0.0122 0.0005
None 0.0228 0.0361 0.0309 0.0036 0.0152 0.0002 0.0175 0.0157 0.0131 0.0002 0.0002 0.0008 0.0141 0.0146 0.0275 0.0011 0.0178 0.0014
Nuclear Periphery 0.0151 0.0093 0.0062 0.0138 0.0018 0.0069 0.0316 0.0139 0.007 0.0048 0.0066 0.0054 0.0181 0.0071 0.0065 0.0085 0.0233 0.0124
Nucleolus 0.0059 0.0069 0.0066 0.0081 0.0075 0.0183 0.0069 0.0068 0.0053 0.0043 0.0206 0.0141 0.0093 0.0043 0.0049 0.0028 0.0104 0.0171
Nucleus 0.7693 0.8917 0.8639 0.871 0.4612 0.943 0.7514 0.9003 0.844 0.9267 0.8792 0.9335 0.7622 0.9036 0.8538 0.911 0.7321 0.9265
Peroxisomes 0.0128 0 0.0066 0.0196 0.0902 0.0012 0.0052 0.0001 0.0026 0.0017 0.0011 0.0009 0.0074 0 0.0135 0.0111 0.0066 0.0002
Punctate Nuclear 0.0244 0.0073 0.023 0.0109 0.0801 0.006 0.0198 0.0127 0.0327 0.0035 0.0062 0.0026 0.018 0.0147 0.0162 0.0015 0.0328 0.0194
Vacuole 0.0044 0.0008 0.0011 0.0039 0.0005 0.0004 0.0137 0.0016 0.0022 0.0002 0.0051 0.0011 0.0036 0.0007 0.0008 0.0004 0.0011 0.0007
Vacuole Periphery 0.0014 0.0001 0.0002 0.0023 0.0004 0.0002 0.0073 0.0003 0.001 0.0002 0.0023 0.0004 0.0022 0.0001 0.0003 0.0003 0.0011 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 67.0026 48.4267 43.4999 79.5355 64.6918 65.5407 70.225 78.2705 80.3513 77.6103
Translational Efficiency 0.897 1.0413 1.2698 0.5987 0.8564 0.9245 1.007 0.8752 0.8662 1.0174

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1992 1745 220 243 1022 1148 151 860 3014 2893 371 1103

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 704.02 774.68 1012.38 1085.51 571.58 770.76 1077.69 989.96 659.11 773.12 1038.96 1011.01
Standard Deviation 84.26 109.27 112.62 130.14 80.02 102.33 120.14 153.67 103.89 106.59 120.11 153.98
Intensity Change Log 2 — 0.137984 0.524063 0.624685 — 0.431326 0.914915 0.792415 — 0.276842 0.712375 0.702262

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000066 0.000369 0.001739 0.003034 0.000149 0.000439 0.006296 0.001005 0.000095 0.000397 0.003594 0.001452
Bud Neck 0.002739 0.005036 0.010298 0.022789 0.003244 0.005494 0.015747 0.018036 0.002910 0.005218 0.012515 0.019083
Bud Site 0.000438 0.001655 0.005046 0.011743 0.001068 0.005322 0.006822 0.009084 0.000651 0.003110 0.005769 0.009670
Cell Periphery 0.000088 0.000120 0.000233 0.000602 0.000265 0.000155 0.000128 0.000216 0.000148 0.000134 0.000190 0.000301
Cytoplasm 0.131554* 0.062411 0.002844 0.009902 0.203687* 0.083484 0.000482 0.020062 0.156013* 0.070773 0.001883 0.017824
Cytoplasmic Foci 0.015225 0.011052 0.000145 0.007407 0.069698 0.017070 0.000097 0.003530 0.033696 0.013440 0.000125 0.004384
Eisosomes 0.000023 0.000031 0.000055 0.000057 0.000177 0.000043 0.000076 0.000032 0.000075 0.000036 0.000064 0.000038
Endoplasmic Reticulum 0.000995 0.002905 0.009620 0.013770 0.000812 0.002139 0.009125 0.005652 0.000933 0.002601 0.009419 0.007441
Endosome 0.000694 0.001151 0.000498 0.024200 0.001649 0.001862 0.000538 0.004052 0.001018 0.001433 0.000514 0.008491
Golgi 0.000128 0.000904 0.000174 0.026250 0.000461 0.000795 0.000265 0.002860 0.000241 0.000861 0.000211 0.008013
Lipid Particles 0.001635 0.002016 0.000471 0.000417 0.010241 0.003409 0.000325 0.000454 0.004553 0.002569 0.000412 0.000445
Mitochondria 0.001236 0.004866 0.001242 0.008724 0.006401 0.007490 0.005282 0.009106 0.002987 0.005907 0.002886 0.009022
Mitotic Spindle 0.000707 0.002506 0.001409 0.027370 0.000307 0.007740 0.006023 0.027194 0.000572 0.004583 0.003287 0.027233
None 0.013117 0.009345 0.006020 0.008363 0.043475 0.011529 0.001660 0.017198 0.023411 0.010212 0.004245 0.015252
Nuclear Periphery 0.000901 0.001519 0.000534 0.002871 0.000877 0.001219 0.000425 0.001387 0.000893 0.001400 0.000490 0.001714
Nuclear Periphery Foci 0.000403 0.000782 0.000406 0.000786 0.000799 0.001013 0.000417 0.000559 0.000538 0.000874 0.000411 0.000609
Nucleolus 0.007530 0.011142 0.012775 0.003545 0.011049 0.012455 0.024957 0.009319 0.008724 0.011663 0.017733 0.008047
Nucleus 0.819295* 0.876656* 0.941383* 0.763293* 0.638775* 0.829665* 0.916813* 0.851658* 0.758084* 0.858009* 0.931382* 0.832190*
Peroxisomes 0.000646 0.002301 0.000604 0.000444 0.003514 0.004110 0.001130 0.000974 0.001619 0.003019 0.000818 0.000857
Vacuole 0.002419 0.002772 0.004312 0.037847 0.003029 0.003840 0.003122 0.013795 0.002626 0.003196 0.003828 0.019094
Vacuole Periphery 0.000159 0.000458 0.000191 0.026587 0.000323 0.000729 0.000270 0.003828 0.000215 0.000566 0.000223 0.008842

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.04 -10.58 -7.75 -6.80 -1.86 -3.11 -2.17 -11.52 -8.03 1.56 -6.61 -3.67 -14.71 -11.16 1.68
Bud Neck -5.43 -7.87 -11.62 -10.54 -5.90 -2.62 -5.61 -12.99 -10.60 -0.27 -5.54 -8.95 -17.01 -14.74 -3.56
Bud Site -3.81 -2.20 -6.68 -5.48 -1.18 -3.03 -2.38 -5.31 -1.22 0.10 -4.30 -3.22 -7.54 -4.22 -0.55
Cell Periphery -2.27 -1.53 -2.41 -1.86 -0.60 3.51 3.75 4.05 0.48 -0.61 0.80 -0.34 -0.81 -1.26 -0.09
Cytoplasm 12.58 28.83 27.14 17.12 -3.95 13.93 28.58 25.75 14.49 -7.71 18.01 39.97 34.87 20.02 -7.54
Cytoplasmic Foci 2.59 13.30 10.77 6.90 -1.78 12.62 18.39 17.89 9.44 -2.32 10.83 21.62 20.08 11.26 -2.74
Eisosomes -4.21 -7.74 -8.74 -6.96 -1.77 1.97 1.50 2.03 0.98 4.83 1.64 0.24 1.09 -4.57 4.08
Endoplasmic Reticulum -10.18 -10.94 -9.08 -7.71 -2.23 -8.38 -11.16 -17.27 -13.10 1.79 -12.71 -15.01 -17.92 -13.83 1.18
Endosome -2.45 0.05 -1.88 -1.68 -1.89 -1.03 4.23 -0.37 0.38 -2.56 -2.58 2.67 -2.42 -1.60 -3.03
Golgi -2.32 -2.07 -1.44 -1.21 -1.41 -1.63 2.79 -1.35 -0.54 -1.85 -2.89 0.43 -2.10 -1.29 -2.12
Lipid Particles -1.51 6.61 6.50 7.12 -0.60 6.12 9.57 9.49 8.17 -0.65 4.76 10.98 10.95 10.98 0.01
Mitochondria -3.81 -0.39 -4.72 -0.05 -4.68 -0.58 1.06 0.77 1.38 -0.50 -3.16 0.68 -1.90 0.77 -2.13
Mitotic Spindle -2.55 -1.52 -3.61 -3.26 -3.23 -4.15 -1.75 -6.05 -3.70 -2.82 -4.80 -2.19 -6.92 -5.50 -4.97
None 2.85 3.88 2.66 1.11 -0.97 7.96 10.98 8.07 1.19 -4.23 8.07 10.51 7.01 1.03 -2.92
Nuclear Periphery -5.93 1.98 -4.43 -3.50 -4.68 -2.79 1.39 -5.85 -3.46 -5.82 -6.39 2.37 -7.20 -4.47 -7.85
Nuclear Periphery Foci -2.55 -0.92 -1.80 -0.10 -0.85 -0.29 1.87 1.81 3.22 -0.35 -1.86 0.74 0.79 3.06 -0.07
Nucleolus -2.96 -3.88 1.65 4.17 4.56 -0.77 -3.05 3.59 3.95 4.42 -2.82 -4.97 2.81 5.20 6.12
Nucleus -7.58 -11.92 -0.59 2.97 6.04 -15.26 -17.69 -18.63 -4.04 2.90 -14.69 -19.67 -11.98 -0.63 6.38
Peroxisomes -3.60 -0.28 3.77 4.61 3.80 -0.30 4.66 5.60 4.83 2.66 -2.77 3.98 5.54 6.30 2.89
Vacuole -1.24 -2.61 -5.37 -5.28 -4.53 -2.26 -1.65 -6.32 -5.90 -5.43 -2.26 -3.04 -8.30 -7.99 -6.45
Vacuole Periphery -3.89 -2.56 -1.50 -1.41 -1.48 -1.49 0.84 -2.23 -1.31 -2.31 -2.99 -1.49 -2.52 -2.07 -2.47
Standard name
Human Ortholog
Description Component of the ADA histone acetyltransferase complex; Ahc2p and Ahc1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ahc1p to the complex
Localization
Cell Percentages nucleus (76%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ahc2

Ahc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ahc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available