Standard name
Human Ortholog
Description Actin-binding protein of the cortical actin cytoskeleton; important for activation of actin nucleation mediated by the Arp2/Arp3 complex; inhibits actin filament elongation at the barbed-end; phosphorylation within its proline-rich region, mediated by Cdc28p and Pho85p, protects Abp1p from PEST sequence-mediated proteolysis; mammalian homolog of HIP-55 (hematopoietic progenitor kinase 1 [HPK1]-interacting protein of 55 kDa)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.33 0.49 0.5 0.48 0.55 0.41 0.38 – 0.32 0.38 0.36 0.33 – – – 0.29 0.3 0.29 0.8 0.74 0.73 0.44 0.08 0.5
Bud 0.11 0.15 0.09 0.15 0.29 0.12 0.12 – 0.08 0.11 0.08 0.14 – – – 0.05 0 0 0.05 0.09 0.08 0.06 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.07 0 0.08 0.06 – 0 0.06 0.06 0 – – – 0 0 0 0 0 0 0 0 0.06
Endoplasmic Reticulum 0 0 0.09 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.25 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.05 0.1 0
Golgi 0.16 0.36 0.57 0.52 0.3 0.24 0.23 – 0.31 0.39 0.13 0.27 – – – 0.69 0.69 0.63 0 0.09 0.08 0 0.06 0.12
Mitochondria 0.78 0.37 0.18 0.35 0.27 0.41 0.53 – 0.66 0.58 0.8 0.7 – – – 0.15 0.25 0.13 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.11 0.09 – 0.1 0 0.05 0.05 – – – 0.06 0.07 0.06 0 0 0 0 0 0
Peroxisomes 0.06 0 0.05 0.05 0.12 0 0.06 – 0.05 0 0.05 0 – – – 0 0 0 0.07 0 0.07 0 0.36 0.05
SpindlePole 0 0 0.05 0 0 0 0 – 0 0 0 0 – – – 0.05 0 0 0 0 0 0 0.11 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 65 70 38 51 69 34 47 – 53 56 48 75 – – – 68 64 63 186 170 76 1 0 9
Bud 21 21 7 16 36 10 15 – 14 17 10 32 – – – 12 5 9 10 20 8 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 1 0 0 0 0
Bud Site 1 0 0 0 0 0 2 – 1 1 1 1 – – – 0 1 1 – – – – – –
Cell Periphery 1 4 2 4 2 4 2 – 1 2 0 2 – – – 2 8 9 0 0 0 0 0 0
Cytoplasm 1 4 5 7 5 7 8 – 7 9 8 8 – – – 6 6 7 0 0 0 0 0 1
Endoplasmic Reticulum 5 5 7 3 3 0 3 – 3 3 1 3 – – – 4 6 8 0 1 0 0 0 0
Endosome 5 2 0 0 0 0 1 – 1 3 0 1 – – – 1 2 2 2 3 1 0 0 0
Golgi 32 51 43 56 38 20 29 – 51 58 17 61 – – – 160 147 137 9 19 8 0 0 2
Mitochondria 153 53 14 37 34 34 66 – 111 87 107 156 – – – 35 54 28 2 4 0 0 0 0
Nucleus 0 0 0 0 0 1 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 1 0 0 1 – 0 0 1 0 – – – 0 0 0 0 1 0 0 0 0
Nucleolus 7 3 1 3 3 9 11 – 16 5 7 12 – – – 13 15 12 0 0 0 0 0 0
Peroxisomes 12 1 4 5 15 3 7 – 8 5 6 9 – – – 2 0 1 17 2 6 0 2 0
SpindlePole 3 3 4 0 4 3 2 – 1 6 2 5 – – – 11 8 9 1 1 1 0 0 1
Vac/Vac Membrane 2 2 2 0 3 0 3 – 2 0 0 0 – – – 3 4 6 0 0 0 0 0 0
Unique Cell Count 196 143 76 107 126 83 125 167 149 133 224 232 212 216 233 229 105 4 7 18
Labelled Cell Count 308 219 128 183 212 125 197 269 252 208 365 317 320 292 233 229 105 4 7 18


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 26.8 39.7 28.6 28.6 29.3 28.9 29.4 – 27.2 28.1 23.4 25.6 – – – 52.2 51.7 47.1 – – –
Std Deviation (1e-4) 10.9 16.9 13.4 9.7 12.0 13.3 10.9 – 9.7 10.7 9.8 10.4 – – – 23.8 23.8 21.5 – – –
Intensity Change (Log2) – – – 0.0 0.03 0.01 0.04 – -0.07 -0.03 -0.29 -0.16 – – – 0.87 0.85 0.72 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.3 0.7 -1.1 -1.7 – -2.7 -1.8 -2.0 -2.6 – – – -3.3 -3.1 -3.3
Bud 1.2 3.3 0.6 0.6 – -0.2 0.5 -0.4 1.1 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cytoplasm 0 0 0.4 0 – 0 0 0 0 – – – 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Golgi -0.6 -3.7 -4.2 -4.8 – -3.9 -2.5 -6.7 -4.6 – – – 2.0 2.0 1.1
Mitochondria 2.4 1.4 3.1 4.8 – 6.9 5.7 8.7 7.8 – – – -0.7 1.2 -1.2
Nucleus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 – 2.3 0 0 0 – – – 0 0 0
Peroxisomes 0 1.6 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.1001 20.8984 22.607 18.6849 18.8607 23.609 25.9431 22.6718 23.6172 27.489 20.8828 25.0494 26.5316 23.6042 24.4211 27.2757 24.1277 26.4015
Actin 0.7973 0.8554 0.882 0.7347 0.858 0.9781 0.8673 0.931 0.9243 0.9791 0.7945 0.9499 0.9095 0.9049 0.9217 0.884 0.9586 0.9519
Bud 0.0006 0.0063 0.0178 0.0029 0.0003 0.0003 0.0004 0.0019 0.0073 0.0065 0.0036 0.0008 0.0017 0.0103 0.005 0.012 0.0009 0.0001
Bud Neck 0.0034 0.0016 0.0031 0.0184 0.0002 0.0009 0.0008 0.0012 0.0053 0.0001 0.0004 0.0033 0.0007 0.0004 0.0023 0.0006 0.0001 0.0012
Bud Periphery 0.0006 0.0067 0.0144 0.0057 0.0003 0.0003 0.0006 0.0017 0.0099 0.0091 0.0063 0.0014 0.0026 0.009 0.0047 0.0125 0.0012 0.0002
Bud Site 0.0094 0.052 0.0303 0.0057 0.0008 0.0005 0.0049 0.024 0.0161 0.0017 0.0026 0.0005 0.0024 0.0145 0.0098 0.0065 0.001 0.0003
Cell Periphery 0.0003 0.001 0.0005 0.0092 0 0 0.0002 0.0003 0.0005 0 0.0006 0.0001 0.0002 0.0004 0.0001 0.0002 0 0.0001
Cytoplasm 0.0035 0.0063 0.0003 0.0055 0.0005 0 0.0006 0.0001 0.0003 0 0.0006 0.0004 0.0001 0.0016 0.0014 0.0107 0 0.0002
Cytoplasmic Foci 0.0292 0.016 0.014 0.0301 0.0329 0.0003 0.0185 0.003 0.0069 0.0001 0.0155 0.0059 0.0045 0.0048 0.0174 0.0134 0.0012 0.0064
Eisosomes 0.0071 0.0008 0.0009 0.0011 0.0011 0.0015 0.011 0.0014 0.0013 0.0004 0.0017 0.0057 0.0022 0.0013 0.0007 0.0012 0.0006 0.0034
Endoplasmic Reticulum 0.0045 0.0008 0.0003 0.0066 0.0001 0 0.0006 0 0.0002 0 0.0003 0.0004 0.0002 0.0001 0.0003 0.0039 0 0
Endosome 0.0199 0.0052 0.0087 0.0158 0.0025 0.0002 0.0033 0.0008 0.0006 0 0.0117 0.0029 0.0033 0.0003 0.0034 0.0256 0.0001 0.0011
Golgi 0.0262 0.0083 0.0039 0.0396 0.0095 0.0108 0.0126 0.0193 0.0012 0.0002 0.0128 0.0056 0.0306 0.0032 0.002 0.0038 0.0012 0.0087
Lipid Particles 0.0169 0.0024 0.0068 0.0074 0.0062 0.0003 0.0144 0.0056 0.0037 0 0.025 0.0041 0.0082 0.0016 0.0057 0.0049 0.0004 0.0029
Mitochondria 0.0114 0.0092 0.0063 0.0584 0.0386 0.0053 0.0344 0.0018 0.0116 0.0017 0.0496 0.0061 0.0147 0.0309 0.0064 0.0013 0.0049 0.0143
None 0.0145 0.0001 0.0003 0.0007 0.0095 0 0.0005 0 0.0006 0 0.0007 0.0003 0.0002 0.0021 0.0025 0.0051 0 0.0004
Nuclear Periphery 0.0085 0.0012 0.0006 0.0174 0.0001 0 0.0022 0.0001 0.0003 0 0.0008 0.0001 0.0003 0.0004 0.0014 0.0044 0 0.0001
Nucleolus 0.0006 0.0004 0.0002 0.0018 0.0003 0 0.0009 0 0.0007 0 0.0057 0.0002 0.0001 0.0015 0.0002 0.0002 0 0.0001
Nucleus 0.0015 0.0007 0.0005 0.0011 0.0001 0 0.0003 0 0.0003 0 0.0008 0.0001 0.0001 0.0028 0.0005 0.0015 0 0
Peroxisomes 0.0379 0.0239 0.0084 0.0092 0.0367 0.0013 0.0249 0.0076 0.007 0.001 0.058 0.0115 0.0154 0.0067 0.011 0.0025 0.0297 0.0058
Punctate Nuclear 0.0042 0.0003 0.0002 0.0004 0.002 0.0001 0.0004 0.0001 0.0017 0 0.0004 0.0004 0.0002 0.0012 0.0029 0.0047 0 0.0026
Vacuole 0.0014 0.0012 0.0004 0.0226 0.0002 0 0.0005 0.0001 0.0002 0 0.0062 0.0002 0.0015 0.001 0.0002 0.0007 0 0.0001
Vacuole Periphery 0.0011 0.0003 0.0002 0.0055 0.0001 0 0.0007 0.0001 0.0001 0 0.0022 0.0001 0.0012 0.0009 0.0001 0.0004 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 176.4579 171.3267 178.5605 142.2607 160.4312 229.9811 173.0881 153.8088 201.2605 159.7638
Translational Efficiency 2.4381 2.3733 2.1358 3.4732 2.764 1.7869 2.0482 2.8558 2.1744 2.4347

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Actin-binding protein of the cortical actin cytoskeleton; important for activation of actin nucleation mediated by the Arp2/Arp3 complex; inhibits actin filament elongation at the barbed-end; phosphorylation within its proline-rich region, mediated by Cdc28p and Pho85p, protects Abp1p from PEST sequence-mediated proteolysis; mammalian homolog of HIP-55 (hematopoietic progenitor kinase 1 [HPK1]-interacting protein of 55 kDa)
Localization
Cell Percentages actin (76%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Abp1

Abp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Abp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available