Standard name | |
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Human Ortholog | |
Description | Subunit of the CCR4-NOT1 core complex; this complex has multiple roles in the regulation of mRNA levels including regulation of transcription and destabilization of mRNA by deadenylation; basal transcription factor that increases initiation and elongation; activates the ATPase activity of Dhh1p, resulting in processing body disassembly |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 3 | 1 | 1 | 2 | 12 | 14 | 26 | 11 | – | 12 | 11 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 6 | 1 | 1 | 1 | 3 | 1 | 3 | – | 2 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 13 | 11 | 8 | 7 | 12 | 11 | 26 | 28 | 24 | – | 26 | 28 | 7 | 7 | 8 | 6 | 10 | 9 | 0 | 0 | 2 | 0 | 0 | 0 |
Cytoplasm | 322 | 521 | 426 | 347 | 379 | 395 | 370 | 367 | 267 | – | 207 | 225 | 289 | 278 | 264 | 189 | 155 | 144 | 326 | 520 | 429 | 153 | 238 | 247 |
Endoplasmic Reticulum | 17 | 26 | 25 | 13 | 45 | 46 | 61 | 68 | 57 | – | 46 | 44 | 9 | 5 | 7 | 8 | 6 | 6 | 10 | 13 | 30 | 12 | 34 | 38 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Golgi | 3 | 0 | 1 | 0 | 0 | 3 | 5 | 4 | 1 | – | 0 | 1 | 0 | 0 | 1 | 4 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 0 |
Mitochondria | 0 | 0 | 3 | 11 | 11 | 59 | 61 | 116 | 126 | – | 104 | 142 | 3 | 0 | 3 | 5 | 1 | 0 | 2 | 0 | 2 | 1 | 0 | 0 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | – | 0 | 0 | 1 | 1 | 0 | 4 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 3 | 1 | 3 | 5 | 1 | 9 | 19 | 4 | – | 2 | 11 | 2 | 0 | 3 | 9 | 11 | 5 | 0 | 7 | 16 | 2 | 4 | 2 |
Unique Cell Count | 338 | 546 | 453 | 363 | 431 | 470 | 493 | 544 | 418 | 321 | 378 | 304 | 285 | 276 | 207 | 173 | 156 | 349 | 548 | 490 | 172 | 287 | 297 | |
Labelled Cell Count | 357 | 564 | 472 | 383 | 455 | 528 | 549 | 633 | 495 | 400 | 469 | 313 | 291 | 291 | 229 | 190 | 170 | 349 | 548 | 490 | 172 | 287 | 297 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.3 | 15.1 | 11.0 | 11.2 | 11.9 | 11.2 | 10.9 | 10.8 | 9.7 | – | 9.5 | 9.7 | 13.9 | 14.0 | 11.8 | 15.4 | 16.6 | 16.1 | 14.9 | 15.3 | 16.2 |
Std Deviation (1e-4) | 3.0 | 2.6 | 1.7 | 1.8 | 1.8 | 1.7 | 1.8 | 2.1 | 1.9 | – | 2.0 | 2.2 | 2.4 | 2.1 | 2.0 | 3.9 | 3.5 | 3.6 | 2.5 | 2.9 | 2.6 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.12 | 0.03 | -0.01 | -0.02 | -0.18 | – | -0.21 | -0.17 | 0.35 | 0.36 | 0.11 | 0.49 | 0.6 | 0.56 | 0.45 | 0.49 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 3.0 | 3.2 | 4.4 | 3.0 | – | 3.8 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.2 | 1.0 | 0.6 | 2.9 | 2.8 | 3.1 | – | 4.2 | 4.0 | 0.5 | 0.6 | 1.0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | -3.2 | -4.8 | -8.0 | -10.4 | -11.0 | – | -10.5 | -12.0 | 0.6 | 2.2 | 0.9 | -1.3 | -1.9 | -0.8 |
Endoplasmic Reticulum | -1.3 | 2.7 | 2.4 | 3.7 | 3.8 | 4.1 | – | 4.2 | 3.2 | -1.7 | -2.5 | -1.9 | -0.9 | -1.1 | -0.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.6 | 2.2 | 7.2 | 7.2 | 10.0 | 12.2 | – | 12.6 | 14.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.7 | 0 | – | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.9218 | 17.9635 | 16.1546 | 16.2597 | 13.3707 | 16.1898 | 9.7447 | 14.1975 | 12.7141 | 13.095 | 12.1504 | 12.7498 | 15.2456 | 18.5806 | 16.8596 | 17.03 | 16.0514 | 17.4245 |
Actin | 0.0492 | 0.0006 | 0.0127 | 0.0015 | 0.014 | 0.0145 | 0.0421 | 0.0023 | 0.0086 | 0.0007 | 0.0083 | 0.0031 | 0.0175 | 0.0011 | 0.0063 | 0.0003 | 0.0129 | 0.0033 |
Bud | 0.0015 | 0.0006 | 0.0011 | 0.0013 | 0.0017 | 0.0006 | 0.0009 | 0.0047 | 0.0007 | 0.0006 | 0.0005 | 0.0002 | 0.0011 | 0.0002 | 0.0007 | 0.0006 | 0.0006 | 0.0003 |
Bud Neck | 0.0077 | 0.0009 | 0.0031 | 0.0016 | 0.0029 | 0.003 | 0.0041 | 0.0003 | 0.0008 | 0.0008 | 0.006 | 0.0008 | 0.0013 | 0.0003 | 0.0008 | 0.0006 | 0.0009 | 0.0014 |
Bud Periphery | 0.0024 | 0.0003 | 0.0013 | 0.0009 | 0.0024 | 0.0007 | 0.0009 | 0.0007 | 0.0005 | 0.0002 | 0.0007 | 0.0001 | 0.0007 | 0.0001 | 0.0005 | 0.0002 | 0.0007 | 0.0002 |
Bud Site | 0.0117 | 0.0016 | 0.003 | 0.0005 | 0.0034 | 0.0004 | 0.0028 | 0.0051 | 0.0006 | 0.0001 | 0.0005 | 0.0002 | 0.0055 | 0.0004 | 0.0057 | 0.0002 | 0.0004 | 0.0002 |
Cell Periphery | 0.0026 | 0.0014 | 0.0045 | 0.0008 | 0.0013 | 0.0014 | 0.0006 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.4387 | 0.929 | 0.7867 | 0.7712 | 0.5706 | 0.6696 | 0.6372 | 0.9286 | 0.8482 | 0.7559 | 0.7438 | 0.8473 | 0.7481 | 0.9554 | 0.8627 | 0.8387 | 0.8622 | 0.8584 |
Cytoplasmic Foci | 0.0223 | 0.0034 | 0.0135 | 0.0064 | 0.0182 | 0.0116 | 0.0195 | 0.0086 | 0.016 | 0.024 | 0.0099 | 0.0149 | 0.0284 | 0.0071 | 0.0136 | 0.0087 | 0.0221 | 0.016 |
Eisosomes | 0.0009 | 0 | 0.0004 | 0 | 0.0008 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.1774 | 0.034 | 0.0728 | 0.0646 | 0.1236 | 0.1787 | 0.0768 | 0.0184 | 0.0404 | 0.0476 | 0.1027 | 0.0444 | 0.0782 | 0.0075 | 0.044 | 0.066 | 0.0248 | 0.0531 |
Endosome | 0.0723 | 0.0086 | 0.0265 | 0.0458 | 0.0785 | 0.0662 | 0.0892 | 0.0145 | 0.0465 | 0.0848 | 0.0677 | 0.0442 | 0.0505 | 0.0125 | 0.0304 | 0.0436 | 0.0418 | 0.0457 |
Golgi | 0.0182 | 0.0009 | 0.0042 | 0.0037 | 0.0217 | 0.0078 | 0.0156 | 0.0031 | 0.0064 | 0.0022 | 0.0059 | 0.0102 | 0.0146 | 0.01 | 0.0091 | 0.0019 | 0.0083 | 0.0087 |
Lipid Particles | 0.028 | 0.0003 | 0.0063 | 0.0002 | 0.0515 | 0.0007 | 0.0088 | 0.0005 | 0.0013 | 0.02 | 0.0021 | 0.0135 | 0.0061 | 0.0001 | 0.0017 | 0.0002 | 0.0023 | 0.0003 |
Mitochondria | 0.054 | 0.0006 | 0.0085 | 0.0025 | 0.0417 | 0.0092 | 0.0159 | 0.0014 | 0.0041 | 0.0014 | 0.0021 | 0.0054 | 0.0116 | 0.0015 | 0.0051 | 0.0012 | 0.0055 | 0.0046 |
None | 0.0072 | 0.0003 | 0.0006 | 0.0004 | 0.0051 | 0.0009 | 0.0046 | 0.001 | 0.001 | 0.0011 | 0.0009 | 0.0005 | 0.0092 | 0.0003 | 0.0009 | 0.0005 | 0.001 | 0.0006 |
Nuclear Periphery | 0.0217 | 0.0023 | 0.008 | 0.0171 | 0.0101 | 0.0115 | 0.0229 | 0.0012 | 0.0034 | 0.0072 | 0.0067 | 0.0027 | 0.0046 | 0.0004 | 0.0025 | 0.0039 | 0.0039 | 0.0013 |
Nucleolus | 0.0015 | 0 | 0.0003 | 0 | 0.0008 | 0.0001 | 0.0068 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0002 | 0 | 0 | 0 |
Nucleus | 0.0171 | 0.0017 | 0.003 | 0.004 | 0.0033 | 0.0031 | 0.0118 | 0.0012 | 0.002 | 0.0021 | 0.0019 | 0.001 | 0.0018 | 0.0005 | 0.0016 | 0.0015 | 0.0017 | 0.0008 |
Peroxisomes | 0.0113 | 0 | 0.0026 | 0.0001 | 0.0102 | 0.0002 | 0.0036 | 0.0009 | 0.0003 | 0.001 | 0.0005 | 0.0029 | 0.0065 | 0.0001 | 0.0051 | 0 | 0.0004 | 0.0002 |
Punctate Nuclear | 0.0042 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0003 | 0.0048 | 0.0002 | 0.0006 | 0.0015 | 0.0032 | 0.0005 | 0.0009 | 0 | 0.0006 | 0.0001 | 0.0003 | 0.0001 |
Vacuole | 0.0405 | 0.0117 | 0.0356 | 0.06 | 0.0256 | 0.0138 | 0.0242 | 0.0058 | 0.0152 | 0.0326 | 0.0278 | 0.0046 | 0.0096 | 0.002 | 0.0068 | 0.0243 | 0.0073 | 0.0032 |
Vacuole Periphery | 0.0096 | 0.0019 | 0.0049 | 0.0171 | 0.0121 | 0.0057 | 0.0067 | 0.001 | 0.0027 | 0.0157 | 0.0084 | 0.0031 | 0.0022 | 0.0003 | 0.0015 | 0.0075 | 0.0025 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 83.0126 | 53.6294 | 49.6285 | 77.8384 | 59.4675 | 59.2619 | 65.4952 | 66.1211 | 58.1168 | 84.2248 |
Translational Efficiency | 0.7109 | 0.7239 | 0.7866 | 0.4692 | 0.6446 | 0.8464 | 0.5961 | 0.4147 | 0.573 | 0.4964 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2404 | 287 | 1627 | 1143 | 1310 | 2018 | 117 | 1449 | 3714 | 2305 | 1744 | 2592 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1124.97 | 1404.94 | 1718.21 | 1612.27 | 1128.53 | 1319.63 | 1699.96 | 1699.73 | 1126.23 | 1330.25 | 1716.99 | 1661.16 |
Standard Deviation | 155.16 | 214.31 | 247.22 | 258.73 | 175.31 | 206.10 | 192.10 | 266.73 | 162.56 | 209.04 | 243.96 | 266.79 |
Intensity Change Log 2 | — | 0.320622 | 0.611020 | 0.519207 | — | 0.225689 | 0.591056 | 0.590861 | — | 0.273861 | 0.601057 | 0.555535 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000124 | 0.000507 | 0.000249 | 0.000397 | 0.000288 | 0.000165 | 0.000971 | 0.000949 | 0.000182 | 0.000208 | 0.000298 | 0.000706 |
Bud Neck | 0.000238 | 0.000728 | 0.000828 | 0.001875 | 0.000318 | 0.000345 | 0.004797 | 0.001229 | 0.000266 | 0.000393 | 0.001094 | 0.001514 |
Bud Site | 0.015891 | 0.043285 | 0.012285 | 0.023258 | 0.017810 | 0.017520 | 0.040502 | 0.028446 | 0.016568 | 0.020728 | 0.014178 | 0.026158 |
Cell Periphery | 0.000471 | 0.000830 | 0.001468 | 0.000983 | 0.000585 | 0.000248 | 0.000879 | 0.005347 | 0.000511 | 0.000321 | 0.001429 | 0.003423 |
Cytoplasm | 0.700188* | 0.573291* | 0.862785* | 0.670627* | 0.722545* | 0.699757* | 0.417631* | 0.812804* | 0.708074* | 0.684010* | 0.832921* | 0.750108* |
Cytoplasmic Foci | 0.171105 | 0.227062* | 0.023901 | 0.049426 | 0.148920 | 0.170483 | 0.136090 | 0.026711 | 0.163280 | 0.177528 | 0.031427 | 0.036728 |
Eisosomes | 0.000006 | 0.000007 | 0.000002 | 0.000005 | 0.000006 | 0.000004 | 0.000013 | 0.000008 | 0.000006 | 0.000005 | 0.000003 | 0.000007 |
Endoplasmic Reticulum | 0.016693 | 0.010279 | 0.002448 | 0.001355 | 0.019415 | 0.008642 | 0.009002 | 0.001821 | 0.017653 | 0.008846 | 0.002888 | 0.001616 |
Endosome | 0.004279 | 0.019359 | 0.003467 | 0.014742 | 0.008780 | 0.009915 | 0.044022 | 0.004341 | 0.005867 | 0.011091 | 0.006188 | 0.008928 |
Golgi | 0.018415 | 0.041087 | 0.026721 | 0.024449 | 0.022860 | 0.028095 | 0.022806 | 0.021427 | 0.019983 | 0.029713 | 0.026458 | 0.022759 |
Lipid Particles | 0.000614 | 0.003177 | 0.004824 | 0.008486 | 0.002538 | 0.001181 | 0.010609 | 0.015705 | 0.001293 | 0.001430 | 0.005212 | 0.012522 |
Mitochondria | 0.002311 | 0.001078 | 0.000123 | 0.001582 | 0.000951 | 0.001347 | 0.000023 | 0.000417 | 0.001831 | 0.001313 | 0.000116 | 0.000931 |
Mitotic Spindle | 0.000430 | 0.005866 | 0.004333 | 0.005533 | 0.000670 | 0.001625 | 0.011770 | 0.001526 | 0.000515 | 0.002153 | 0.004832 | 0.003293 |
None | 0.001220 | 0.000111 | 0.000479 | 0.000165 | 0.000453 | 0.000725 | 0.000048 | 0.000287 | 0.000950 | 0.000648 | 0.000450 | 0.000233 |
Nuclear Periphery | 0.000050 | 0.000142 | 0.000137 | 0.000154 | 0.000074 | 0.000030 | 0.000264 | 0.000032 | 0.000059 | 0.000044 | 0.000145 | 0.000086 |
Nuclear Periphery Foci | 0.000693 | 0.001580 | 0.007587 | 0.003114 | 0.002050 | 0.000519 | 0.009845 | 0.002681 | 0.001171 | 0.000651 | 0.007738 | 0.002872 |
Nucleolus | 0.000055 | 0.000053 | 0.000030 | 0.000312 | 0.000072 | 0.000046 | 0.000051 | 0.000067 | 0.000061 | 0.000047 | 0.000032 | 0.000175 |
Nucleus | 0.002003 | 0.001169 | 0.002996 | 0.004010 | 0.002003 | 0.001345 | 0.003206 | 0.001838 | 0.002003 | 0.001323 | 0.003010 | 0.002796 |
Peroxisomes | 0.000171 | 0.000454 | 0.000078 | 0.001127 | 0.000305 | 0.000324 | 0.000811 | 0.000423 | 0.000219 | 0.000340 | 0.000127 | 0.000734 |
Vacuole | 0.013127 | 0.037588 | 0.029671 | 0.166487 | 0.024469 | 0.029221 | 0.273616* | 0.063070 | 0.017128 | 0.030263 | 0.046037 | 0.108674 |
Vacuole Periphery | 0.051915 | 0.032347 | 0.015588 | 0.021913 | 0.024885 | 0.028461 | 0.013045 | 0.010871 | 0.042381 | 0.028945 | 0.015418 | 0.015740 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.06 | -4.78 | -7.05 | 1.22 | -3.03 | 2.74 | -3.90 | -3.07 | -3.69 | 0.12 | -1.15 | -3.98 | -4.34 | -4.14 | -3.24 |
Bud Neck | -6.38 | -7.38 | -12.20 | -7.94 | -6.65 | -0.78 | -7.37 | -9.21 | -8.76 | 5.80 | -4.15 | -9.43 | -15.38 | -13.39 | -3.39 |
Bud Site | -5.91 | 2.95 | -4.30 | 4.13 | -5.77 | 0.23 | -4.47 | -5.72 | -5.99 | 2.19 | -3.94 | 1.99 | -7.60 | -3.75 | -7.82 |
Cell Periphery | -2.82 | -8.06 | -4.75 | -0.58 | 3.85 | 3.73 | -1.35 | -8.98 | -9.77 | -8.07 | 4.28 | -7.80 | -9.61 | -10.33 | -6.08 |
Cytoplasm | 6.59 | -20.85 | 3.02 | -4.62 | 18.37 | 2.31 | 10.71 | -8.69 | -12.13 | -13.88 | 3.19 | -16.55 | -5.41 | -7.64 | 10.46 |
Cytoplasmic Foci | -4.61 | 36.03 | 27.58 | 15.30 | -9.52 | -3.37 | 1.08 | 24.29 | 32.13 | 8.93 | -2.84 | 38.59 | 38.51 | 33.79 | -2.21 |
Eisosomes | -0.90 | 9.31 | 1.64 | 1.98 | -6.70 | 2.80 | -4.53 | -0.89 | -1.87 | 2.36 | 3.58 | 9.04 | -0.36 | -1.84 | -3.84 |
Endoplasmic Reticulum | 2.58 | 12.04 | 13.52 | 4.05 | 3.25 | 5.13 | 4.41 | 9.15 | 6.94 | 4.18 | 6.75 | 14.13 | 15.89 | 8.30 | 3.30 |
Endosome | -6.87 | 2.51 | -8.97 | 2.26 | -9.49 | -1.48 | -8.66 | 6.42 | 8.48 | 9.83 | -8.96 | -0.67 | -4.76 | 3.36 | -3.55 |
Golgi | -7.63 | -5.49 | -1.80 | 5.34 | 1.90 | -3.52 | 0.32 | 1.04 | 3.75 | 0.11 | -8.11 | -4.49 | -1.23 | 5.01 | 2.70 |
Lipid Particles | -2.88 | -9.88 | -9.65 | -4.41 | -3.82 | 3.33 | -6.01 | -11.94 | -13.55 | -2.85 | -0.54 | -9.28 | -15.98 | -15.28 | -8.88 |
Mitochondria | 1.89 | 14.52 | 1.46 | -0.36 | -2.09 | -1.05 | 2.83 | 1.50 | 4.02 | -2.58 | 2.25 | 11.26 | 3.09 | 1.42 | -2.59 |
Mitotic Spindle | -2.14 | -6.05 | -3.90 | 0.12 | -0.68 | -3.78 | -4.32 | -2.06 | 0.12 | 3.91 | -4.31 | -6.85 | -4.48 | -1.58 | 1.85 |
None | 3.89 | 2.59 | 3.71 | -1.50 | 7.31 | -0.66 | 4.88 | 1.34 | 1.06 | -2.50 | 0.75 | 2.65 | 3.70 | 1.17 | 3.51 |
Nuclear Periphery | -1.04 | -3.87 | -3.64 | -0.19 | -0.51 | 2.60 | -4.33 | 2.46 | -0.63 | 5.63 | 0.98 | -4.18 | -1.97 | -2.63 | 2.58 |
Nuclear Periphery Foci | -2.53 | -11.62 | -6.77 | -4.32 | 7.10 | 5.90 | -6.59 | -2.24 | -12.27 | 6.09 | 2.81 | -12.08 | -7.45 | -13.55 | 8.93 |
Nucleolus | 0.15 | 2.17 | -2.10 | -2.10 | -2.35 | 1.39 | 0.92 | 0.18 | -2.54 | -1.59 | 1.25 | 3.03 | -2.09 | -2.41 | -2.73 |
Nucleus | 1.70 | -2.64 | -5.95 | -7.79 | -4.81 | 1.50 | -2.43 | 0.00 | -3.83 | 3.34 | 2.36 | -3.38 | -3.94 | -9.90 | -0.57 |
Peroxisomes | -4.93 | 7.84 | -3.30 | -2.22 | -3.64 | -0.22 | -3.01 | -1.33 | -1.77 | 2.56 | -2.56 | 2.73 | -3.91 | -2.89 | -4.72 |
Vacuole | -4.35 | -8.15 | -24.67 | -15.93 | -21.17 | -1.94 | -9.64 | -10.75 | -9.69 | 7.96 | -7.31 | -9.77 | -26.17 | -21.15 | -14.41 |
Vacuole Periphery | 4.51 | 15.23 | 12.10 | 3.02 | -2.21 | -1.68 | 4.38 | 7.70 | 10.62 | 0.64 | 6.79 | 14.79 | 15.97 | 9.00 | 0.43 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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