Standard name
Human Ortholog
Description Cytoplasmic protein required for sporulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.15 0.18 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0.07 0.15 0.25 0.12 0.13 0.2 0.26 0.24 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.89 0.89 0.88 0.91 0.84 0.77 0.72 0.69 0.71 0.64 0.65 0.62 0.82 0.79 0.73 – – – 0.78 0.83 0.73 0.76 0.76 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.23 0.22 0.18 0.17 0.2 0.27 0.28 0.36 0.39 0.41 0.42 0.39 0.12 0.08 0.1 – – – 0.15 0.11 0.19 0.13 0.12 0.21
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – – – 0 0 3 0 0 0
Bud 0 0 0 0 1 1 4 4 1 3 1 3 0 0 1 – – – 1 2 3 4 3 5
Bud Neck 0 0 0 0 2 0 1 1 2 2 1 1 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 2 1 10 8 11 9 18 17 13 19 0 1 2 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 3 1 19 13 26 19 28 24 17 9 12 11 47 58 69 – – – 0 0 1 4 7 3
Endoplasmic Reticulum 1 0 0 0 0 1 0 0 0 0 0 0 1 0 1 – – – 0 0 2 0 0 0
Endosome 0 1 3 1 2 1 0 1 1 0 0 0 2 2 4 – – – 2 0 6 4 7 8
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 – – – 1 0 2 1 0 1
Mitochondria 4 9 6 11 45 84 176 96 96 183 132 115 1 2 0 – – – 3 0 1 2 4 4
Nucleus 187 134 506 365 517 418 518 567 526 583 335 295 302 303 280 – – – 173 136 462 280 318 315
Nuclear Periphery 2 0 3 2 4 3 10 13 10 7 5 3 2 4 4 – – – 0 0 2 1 1 0
Nucleolus 49 33 101 69 126 147 202 296 286 375 218 186 46 30 37 – – – 32 17 120 48 49 100
Peroxisomes 1 0 0 0 1 0 1 0 0 1 0 0 2 0 2 – – – 0 0 1 0 0 0
SpindlePole 1 0 1 0 4 1 1 1 1 1 1 0 5 4 16 – – – 0 1 3 3 5 5
Vac/Vac Membrane 0 4 4 1 8 3 2 1 3 3 3 3 4 4 5 – – – 4 3 16 10 18 29
Unique Cell Count 210 150 576 401 618 545 717 819 741 908 517 475 368 384 381 222 164 634 368 421 480
Labelled Cell Count 248 182 646 463 746 686 954 1013 961 1184 721 636 412 410 421 222 164 634 368 421 480


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 7.9 7.6 6.9 6.5 6.1 5.8 5.9 6.0 5.8 5.5 5.6 9.5 9.2 8.9 – – – 9.4 9.7 10.4
Std Deviation (1e-4) 1.3 1.8 1.7 1.7 1.7 1.7 1.8 1.5 1.6 1.5 1.7 1.5 2.8 2.5 2.7 – – – 2.3 2.3 2.2
Intensity Change (Log2) – – – -0.13 -0.22 -0.32 -0.39 -0.36 -0.35 -0.39 -0.45 -0.44 0.33 0.28 0.24 – – – 0.32 0.35 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 2.2 0 2.1 0 3.1 2.5 3.1 4.2 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0 0.8 0.2 0.6 -0.4 -1.1 -3.2 -1.0 -1.0 5.6 6.6 7.8 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 2.0 5.3 8.9 12.1 7.5 8.0 10.8 12.2 11.7 0 0 0 – – –
Nucleus 1.6 -2.1 -4.9 -6.9 -8.1 -7.4 -10.0 -9.0 -9.8 -2.5 -3.7 -5.7 – – –
Nuclear Periphery 0 0 0 1.6 1.8 1.5 0 0 0 0 0 0 – – –
Nucleolus -0.1 1.3 3.8 4.5 7.6 8.3 9.6 8.9 7.8 -2.1 -4.3 -3.4 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 4.6 – – –
Vacuole 0 1.0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.197 8.8234 8.947 9.454 8.4616 8.7182 5.776 6.6162 6.2662 5.3501 5.1521 6.0644 4.6959 6.0861 5.1037 4.8216 4.8766 5.0037
Actin 0.0081 0 0.0138 0.0036 0.0013 0.0026 0.0357 0 0.0145 0.0393 0.0004 0.0008 0.0313 0 0.0204 0.0107 0.0056 0.0061
Bud 0.0002 0 0.0005 0.0002 0.0001 0 0.0009 0 0.001 0.0087 0.0004 0.0001 0.0004 0 0.0011 0.0001 0.0006 0.0001
Bud Neck 0.001 0.0001 0.0025 0.0019 0.0005 0.0004 0.008 0 0.0023 0.0011 0.0008 0.0008 0.0008 0 0.0003 0.0001 0.0004 0.0008
Bud Periphery 0.0003 0 0.0012 0.0002 0.0002 0.0001 0.0011 0 0.0018 0.0054 0.0007 0.0001 0.0004 0 0.0012 0.0001 0.0012 0.0002
Bud Site 0.0007 0 0.0076 0.0292 0.0004 0.0001 0.0067 0 0.0083 0.0019 0.0004 0.0001 0.0025 0 0.0064 0.0004 0.0014 0.0002
Cell Periphery 0.0002 0 0.0004 0.0002 0.0001 0 0.0003 0 0.0002 0.0003 0.0001 0 0.0002 0 0.0002 0 0.0001 0.0001
Cytoplasm 0.0071 0 0.0018 0.0003 0.0048 0 0.009 0.0004 0.0001 0.0008 0.0002 0 0.0108 0 0.0013 0.0002 0.0003 0.0001
Cytoplasmic Foci 0.0051 0 0.004 0.0004 0.0011 0.0003 0.0463 0 0.0045 0.0047 0.0036 0.0003 0.0737 0 0.0204 0.0274 0.0149 0.0056
Eisosomes 0.0001 0 0.0001 0.0001 0.0001 0 0.0004 0 0.0002 0.0002 0 0 0.0001 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0033 0 0.001 0.0001 0.0051 0.0001 0.0032 0.0003 0.0002 0.0007 0.0004 0.0001 0.0032 0 0.0008 0.0001 0.0005 0
Endosome 0.0089 0 0.0026 0.0001 0.0264 0.0008 0.0291 0.0001 0.0027 0.0054 0.0232 0.0021 0.0334 0 0.0124 0.0025 0.0162 0.0053
Golgi 0.0025 0 0.0012 0.0001 0.0018 0.0009 0.016 0 0.0042 0.0066 0.0068 0.0047 0.0123 0 0.0153 0.0055 0.0151 0.0167
Lipid Particles 0.0225 0 0.0015 0.0001 0.0102 0.0012 0.0225 0 0.0072 0.0074 0.0344 0.0018 0.0246 0 0.0118 0.0571 0.0194 0.0153
Mitochondria 0.0016 0.0001 0.0027 0.0001 0.0015 0.0002 0.0145 0.0002 0.01 0.0061 0.0069 0.0012 0.0054 0.0001 0.014 0.0022 0.0607 0.0085
None 0.0037 0 0.0014 0.0001 0.003 0 0.0025 0.0001 0.0001 0.0006 0.0001 0 0.0033 0 0.0004 0.0002 0.0005 0.0001
Nuclear Periphery 0.0171 0.0007 0.0039 0.0003 0.0308 0.0004 0.0079 0.0031 0.0003 0.0111 0.0018 0.0002 0.0213 0.0002 0.0016 0.001 0.0029 0.0001
Nucleolus 0.0564 0.0362 0.0252 0.0186 0.1653 0.1122 0.0281 0.0164 0.0145 0.0224 0.1674 0.0898 0.031 0.0217 0.0162 0.0097 0.1464 0.1424
Nucleus 0.8323 0.9435 0.9228 0.9436 0.7289 0.8795 0.7113 0.9786 0.9221 0.8675 0.7414 0.8975 0.669 0.9778 0.852 0.8079 0.6375 0.7729
Peroxisomes 0.0009 0 0.0018 0.0002 0.0003 0.0004 0.0167 0 0.005 0.0043 0.0006 0.0001 0.0107 0 0.021 0.0724 0.0666 0.0244
Punctate Nuclear 0.0252 0.0194 0.0029 0.0003 0.0124 0.0008 0.0358 0.0003 0.0003 0.0031 0.0026 0.0002 0.0599 0.0001 0.0008 0.0017 0.0073 0.0006
Vacuole 0.0018 0 0.0008 0.0001 0.0034 0.0001 0.0025 0.0002 0.0004 0.0013 0.0038 0.0001 0.0038 0 0.0013 0.0001 0.0008 0.0002
Vacuole Periphery 0.0011 0 0.0004 0 0.0024 0 0.0013 0.0002 0.0002 0.0012 0.0038 0.0001 0.0021 0 0.001 0.0007 0.0017 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.4003 4.5772 7.925 11.8835 12.1707 10.2347 9.4487 8.5898 12.2285 13.2025
Translational Efficiency 2.7517 3.1886 1.4866 1.3308 1.2588 1.9282 1.2971 1.6295 1.066 1.3869

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1674 1688 1608 110 2285 2128 423 345 3959 3816 2031 455

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 836.17 945.09 1019.15 1260.26 874.04 933.52 1123.62 1568.64 858.03 938.64 1040.91 1494.09
Standard Deviation 122.96 159.17 147.73 205.36 121.34 136.35 162.90 332.89 123.46 147.00 156.86 334.15
Intensity Change Log 2 — 0.176655 0.285498 0.591853 — 0.094982 0.362383 0.843743 — 0.135492 0.325304 0.726068

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000404 0.000538 0.000789 0.001803 0.000334 0.000558 0.001164 0.000358 0.000364 0.000549 0.000867 0.000707
Bud Neck 0.004348 0.007362 0.017036 0.015204 0.005055 0.008257 0.010144 0.046070 0.004756 0.007861 0.015601 0.038608
Bud Site 0.000487 0.000935 0.001212 0.002146 0.000565 0.001829 0.001120 0.002973 0.000532 0.001434 0.001193 0.002773
Cell Periphery 0.000048 0.000086 0.000053 0.000060 0.000037 0.000098 0.000024 0.000114 0.000041 0.000093 0.000047 0.000101
Cytoplasm 0.000577 0.003154 0.000614 0.000333 0.000360 0.001094 0.000095 0.006361 0.000452 0.002005 0.000506 0.004904
Cytoplasmic Foci 0.000120 0.000110 0.000025 0.001613 0.000424 0.000342 0.000625 0.000908 0.000295 0.000239 0.000150 0.001079
Eisosomes 0.000031 0.000042 0.000050 0.000059 0.000043 0.000048 0.000052 0.000028 0.000038 0.000045 0.000051 0.000036
Endoplasmic Reticulum 0.001974 0.001718 0.002376 0.003236 0.001868 0.002503 0.002566 0.002668 0.001913 0.002156 0.002416 0.002806
Endosome 0.000202 0.000165 0.000129 0.002374 0.000203 0.000839 0.000570 0.000901 0.000203 0.000541 0.000221 0.001257
Golgi 0.000177 0.000126 0.000120 0.017682 0.000446 0.000277 0.000317 0.002545 0.000332 0.000210 0.000161 0.006204
Lipid Particles 0.000086 0.000040 0.000038 0.000132 0.000154 0.000185 0.000058 0.000036 0.000125 0.000121 0.000042 0.000059
Mitochondria 0.001135 0.000818 0.001248 0.003407 0.000762 0.002063 0.001270 0.002806 0.000920 0.001512 0.001253 0.002951
Mitotic Spindle 0.001381 0.002872 0.001464 0.013254 0.000580 0.003103 0.001403 0.004478 0.000919 0.003001 0.001451 0.006600
None 0.003178 0.004812 0.001993 0.001407 0.000873 0.002266 0.001122 0.002091 0.001848 0.003392 0.001811 0.001926
Nuclear Periphery 0.000138 0.000121 0.000196 0.000853 0.000099 0.000294 0.000090 0.003522 0.000116 0.000217 0.000174 0.002877
Nuclear Periphery Foci 0.000917 0.000506 0.000127 0.000823 0.000153 0.000483 0.000070 0.000149 0.000476 0.000493 0.000115 0.000312
Nucleolus 0.101317 0.081551 0.070625 0.081981 0.098515 0.092512 0.087582 0.010181 0.099700 0.087663 0.074157 0.027539
Nucleus 0.882518* 0.893849* 0.900684* 0.832741* 0.888489* 0.880612* 0.889147* 0.907536* 0.885964* 0.886468* 0.898281* 0.889454*
Peroxisomes 0.000594 0.000696 0.000767 0.002566 0.000598 0.000765 0.001909 0.000120 0.000596 0.000734 0.001004 0.000711
Vacuole 0.000178 0.000295 0.000312 0.006336 0.000261 0.001182 0.000470 0.004051 0.000225 0.000790 0.000345 0.004603
Vacuole Periphery 0.000189 0.000202 0.000140 0.011991 0.000183 0.000689 0.000204 0.002102 0.000185 0.000474 0.000154 0.004493

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.05 -13.05 -10.16 -9.48 -7.73 -3.54 -8.99 -1.09 2.92 8.74 -4.84 -15.44 -7.18 -4.47 1.31
Bud Neck -6.30 -17.50 -5.65 -4.45 0.16 -6.22 -6.55 -9.49 -8.78 -7.95 -8.70 -18.80 -10.19 -9.33 -6.64
Bud Site -2.84 -7.97 -5.26 -4.29 -3.45 -2.43 -2.94 -3.54 -1.72 -2.56 -3.06 -8.23 -4.58 -2.88 -3.14
Cell Periphery -2.05 -0.61 -2.19 0.57 -2.23 -2.74 2.46 -4.01 0.01 -4.86 -3.44 -1.23 -4.19 0.05 -3.76
Cytoplasm -3.00 -0.87 0.12 3.05 0.99 -1.53 1.06 -2.98 -2.57 -3.13 -3.35 -0.85 -2.95 -1.80 -2.76
Cytoplasmic Foci -0.08 1.95 -1.04 -1.03 -1.19 0.22 -0.19 -0.42 -0.56 -0.19 0.23 0.86 -0.85 -0.97 -1.23
Eisosomes -5.08 -12.42 -7.82 -5.46 -3.49 -2.14 -4.44 5.45 6.83 8.20 -4.49 -9.98 0.72 3.27 6.13
Endoplasmic Reticulum 1.91 -3.43 -4.46 -5.09 -3.44 -3.47 -2.88 -2.42 0.09 0.65 -2.09 -5.30 -4.22 -2.98 -1.46
Endosome 0.42 0.94 -1.52 -1.58 -1.63 -2.60 -0.68 -1.75 0.31 -0.45 -2.41 -0.10 -2.31 -0.97 -2.19
Golgi 0.97 0.88 -1.39 -1.40 -1.40 0.74 0.60 -0.81 -0.92 -0.91 0.91 1.27 -1.59 -1.66 -1.68
Lipid Particles 1.50 1.53 -2.01 -3.83 -3.89 -0.36 1.45 1.85 2.21 1.62 0.06 2.08 1.37 1.30 -2.09
Mitochondria 0.65 -0.49 -3.65 -5.31 -4.40 -2.36 -2.88 -3.84 -1.27 -2.77 -1.65 -1.97 -4.53 -3.03 -3.93
Mitotic Spindle -1.41 -1.04 -2.73 -2.61 -2.68 -2.74 -0.79 -1.76 -0.56 -1.34 -2.98 -2.03 -3.14 -2.55 -2.85
None -1.54 1.84 1.66 2.88 -0.06 -4.14 -1.76 -5.28 -0.71 -4.58 -3.10 -0.07 -1.75 1.87 -2.00
Nuclear Periphery 0.13 -1.68 -3.07 -3.11 -2.83 -2.25 -0.22 -1.78 -1.66 -1.77 -1.90 -2.70 -1.97 -1.88 -1.90
Nuclear Periphery Foci 0.85 1.84 0.17 -0.73 -1.86 -2.41 2.05 -0.36 2.02 -1.44 -0.23 1.63 0.76 1.15 -1.19
Nucleolus 4.95 6.26 1.59 -0.08 -0.60 1.62 1.25 20.42 18.88 11.53 4.44 6.48 15.54 12.80 9.83
Nucleus -2.16 -2.00 2.86 3.30 3.26 1.95 0.68 -0.71 -1.53 -1.03 0.09 -1.14 0.99 0.95 1.39
Peroxisomes -1.47 -2.96 -4.98 -4.67 -4.31 -0.92 -7.04 4.00 4.35 11.10 -1.27 -5.16 -0.12 0.98 4.07
Vacuole -1.85 -2.59 -2.54 -2.46 -2.41 -3.21 -2.71 -2.20 -1.66 -2.04 -3.50 -2.65 -2.86 -2.44 -2.71
Vacuole Periphery -0.37 1.37 -1.21 -1.21 -1.22 -3.42 -0.49 -1.30 -0.81 -1.27 -3.40 0.92 -1.76 -1.53 -1.78
Standard name
Human Ortholog
Description Cytoplasmic protein required for sporulation
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rmd1

Rmd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rmd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available