Standard name
Human Ortholog
Description Nonfunctional homolog of Gpm1p phosphoglycerate mutase; if functional, would convert 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; GPM2 has a paralog, GPM3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 1.0 0.99 0.97 0.93 0.79 0.82 0.74 0.72 0.74 0.67 0.99 0.99 0.98 0.95 0.97 0.92 0.91 0.68 0.79 0.9 0.9 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0 0 0 0.06 0.13 0.09 0.2 0.17 0.26 0.18 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0.16 0.08 0.11 0.17 0.09 0.19 0 0 0 0 0 0 0 0.25 0.08 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 3 4
Bud 0 1 0 0 0 0 0 5 6 3 5 2 0 0 0 0 1 2 0 2 0 2 6 29
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 2 2 1 0 1 2 0 0 0 0 1 5
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 0 3 6 6 4 4 7 4 4 3 2 4 6 14 5 0 0 0 0 0 1
Cytoplasm 105 124 22 207 291 303 336 309 265 286 217 219 447 539 653 145 207 163 104 96 62 165 404 459
Endoplasmic Reticulum 0 1 0 0 0 0 2 5 4 5 1 7 1 3 1 9 13 9 0 0 0 1 0 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 2 5 1 0 0 1 2 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 3 0 0 0 0 0 1
Mitochondria 18 3 0 0 2 18 57 32 73 68 76 59 0 0 0 3 4 4 2 1 3 2 8 10
Nucleus 0 1 0 1 3 10 7 12 9 8 7 10 1 0 3 0 1 1 0 2 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0 0 1 8 18
Vac/Vac Membrane 0 0 0 1 7 15 69 31 39 69 27 62 2 7 10 4 2 5 0 36 6 1 2 3
Unique Cell Count 110 127 22 209 300 327 426 376 360 398 295 326 453 543 666 152 214 178 115 143 79 183 447 555
Labelled Cell Count 123 132 22 211 306 352 479 399 401 446 338 363 457 553 675 170 248 201 115 143 79 183 447 555


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 5.6 4.8 5.2 6.3 6.3 6.5 6.8 6.2 6.8 6.2 6.6 5.6 7.1 8.1 6.1 6.2 5.8 5.2 5.5 6.5
Std Deviation (1e-4) 0.7 1.4 0.8 1.6 1.7 1.8 1.7 1.6 1.5 1.8 1.7 1.7 1.0 1.8 1.9 2.0 2.1 2.1 1.3 1.2 1.7
Intensity Change (Log2) – – – 0.11 0.39 0.4 0.43 0.5 0.37 0.49 0.35 0.46 0.22 0.56 0.75 0.34 0.36 0.28 0.12 0.2 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -0.8 -1.3 -2.4 -2.2 -2.8 -2.9 -2.8 -3.2 -0.5 -0.4 -0.7 -1.0 -0.9 -1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.7 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.904 -0.1937 -0.4848 -0.8251 -0.6326 -0.3771 0.0331 0.0494 0.1812 -0.0657 -0.7033 -0.0834 -0.6628 -0.3145 -0.2912 -0.6421 -0.6178 -0.6822
Actin 0.0109 0.0005 0.0087 0.0021 0.0003 0.0022 0.0159 0.0004 0.0097 0.0156 0.0016 0.002 0.0563 0.0014 0.0369 0.0025 0.0035 0.0069
Bud 0.0056 0.0033 0.0007 0.0003 0.0001 0.0046 0.0007 0.0025 0.0017 0.0004 0.0039 0.0035 0.0019 0.0025 0.0065 0.008 0.0006 0.001
Bud Neck 0.0064 0.0001 0.0003 0.0001 0.0005 0.0017 0.0141 0.0001 0.0014 0.0024 0.0001 0.0008 0.0043 0.0002 0.0048 0.0002 0.0002 0.0006
Bud Periphery 0.0021 0.0017 0.0008 0.0009 0.0001 0.0017 0.0014 0.0013 0.0035 0.0005 0.0069 0.001 0.0037 0.0009 0.003 0.0037 0.0003 0.0004
Bud Site 0.0075 0.0027 0.0096 0.0001 0.0009 0.0021 0.0139 0.0051 0.007 0.0032 0.001 0.0033 0.0052 0.012 0.0157 0.0025 0.0005 0.0001
Cell Periphery 0.0002 0.0001 0.0001 0 0.0001 0.0002 0.0008 0.0001 0.0004 0.0001 0.0001 0.0001 0.0023 0.0002 0.0004 0.0002 0 0
Cytoplasm 0.0967 0.2027 0.161 0.066 0.1748 0.2009 0.11 0.1545 0.1625 0.0809 0.1359 0.229 0.2041 0.4011 0.3301 0.3132 0.2901 0.3754
Cytoplasmic Foci 0.0056 0.0058 0.0113 0.0098 0.0073 0.0154 0.0286 0.0045 0.0088 0.0073 0.0069 0.0085 0.0208 0.0187 0.0103 0.0234 0.0399 0.015
Eisosomes 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0.0002 0.0001 0 0.0006 0.0001 0.0005 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0016 0.0008 0.0012 0.0007 0.0011 0.0008 0.0015 0.001 0.0011 0.0003 0.0005 0.0005 0.003 0.0026 0.0029 0.0027 0.0012 0.0012
Endosome 0.0072 0.0011 0.0056 0.0014 0.0039 0.0024 0.0197 0.0006 0.0083 0.0037 0.0029 0.0017 0.0155 0.008 0.0074 0.0081 0.0047 0.0017
Golgi 0.0015 0.0001 0.0012 0.0012 0.0005 0.0003 0.0053 0.0001 0.0028 0.0046 0.0045 0.0009 0.0082 0.002 0.0033 0.0022 0.0019 0.0004
Lipid Particles 0.0024 0.0001 0.0009 0.0158 0.0032 0.0003 0.0181 0.0003 0.0073 0.0045 0.0067 0.0029 0.0133 0.0002 0.0035 0.0021 0.0076 0.0002
Mitochondria 0.0035 0.0007 0.001 0.0008 0.0007 0.0006 0.0062 0.0004 0.0034 0.0022 0.0246 0.0009 0.0081 0.0008 0.0012 0.0239 0.0013 0.0004
None 0.8372 0.7739 0.784 0.8739 0.7714 0.7504 0.7306 0.8204 0.7538 0.8654 0.7776 0.7372 0.6154 0.5426 0.559 0.5877 0.5949 0.5876
Nuclear Periphery 0.0026 0.0005 0.0012 0.0016 0.0019 0.003 0.0037 0.0007 0.0014 0.0003 0.0003 0.0004 0.004 0.0009 0.0023 0.0022 0.0011 0.0006
Nucleolus 0.0009 0.0006 0.0004 0.0002 0.0162 0.0006 0.0009 0.0005 0.0009 0.0003 0.0003 0.0008 0.0022 0.0002 0.0007 0.0012 0.0004 0.0003
Nucleus 0.003 0.0023 0.0025 0.0014 0.0077 0.0045 0.0013 0.0025 0.01 0.0006 0.0016 0.0024 0.0056 0.0024 0.0028 0.0052 0.0076 0.0024
Peroxisomes 0.0004 0.0003 0.003 0.0194 0.0003 0.0007 0.0139 0.0003 0.0065 0.0055 0.0231 0.0022 0.0107 0.0014 0.0029 0.0046 0.0362 0.0002
Punctate Nuclear 0.0027 0.0018 0.0051 0.0038 0.0069 0.0069 0.0099 0.0043 0.0058 0.0012 0.0007 0.0015 0.0088 0.0008 0.0046 0.0045 0.0066 0.0049
Vacuole 0.0011 0.0006 0.0009 0.0004 0.0016 0.0006 0.0022 0.0004 0.0026 0.0007 0.0006 0.0005 0.0049 0.0009 0.001 0.0015 0.001 0.0005
Vacuole Periphery 0.0006 0.0001 0.0002 0.0002 0.0005 0.0002 0.0009 0.0001 0.0007 0.0002 0.0001 0.0001 0.0012 0.0002 0.0003 0.0005 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.7683 7.3767 6.2558 3.8657 3.0569 1.1782 8.5241 6.8319 6.757 6.0167
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2200 272 2762 1299 1807 935 833 1902 4007 1207 3595 3201

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 761.95 808.30 885.92 816.76 702.68 735.82 1140.66 899.04 735.22 752.15 944.95 865.65
Standard Deviation 103.07 123.13 136.39 158.85 96.85 111.88 195.92 149.14 104.56 118.45 186.38 158.39
Intensity Change Log 2 — 0.085195 0.217480 0.100216 — 0.066485 0.698929 0.355517 — 0.076249 0.468511 0.228345

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000108 0.000813 0.000199 0.000636 0.000202 0.000722 0.000375 0.000325 0.000151 0.000742 0.000240 0.000451
Bud Neck 0.010087 0.024498 0.005286 0.006563 0.015985 0.032173 0.002139 0.005803 0.012746 0.030443 0.004557 0.006112
Bud Site 0.004495 0.030743 0.010458 0.025343 0.010538 0.024674 0.010697 0.026860 0.007220 0.026042 0.010513 0.026244
Cell Periphery 0.000112 0.000180 0.000080 0.000167 0.000178 0.000189 0.000134 0.000114 0.000142 0.000187 0.000092 0.000135
Cytoplasm 0.621974* 0.416971* 0.563947* 0.527838* 0.552751* 0.399927* 0.599457* 0.611806* 0.590757* 0.403768* 0.572175* 0.577731*
Cytoplasmic Foci 0.157661 0.247291* 0.005734 0.031578 0.215388* 0.271285* 0.011593 0.019768 0.183693* 0.265878* 0.007091 0.024561
Eisosomes 0.000101 0.000141 0.000013 0.000050 0.000204 0.000179 0.000030 0.000033 0.000147 0.000170 0.000017 0.000040
Endoplasmic Reticulum 0.000685 0.001281 0.001055 0.001310 0.001528 0.000971 0.002516 0.000751 0.001065 0.001041 0.001394 0.000978
Endosome 0.008454 0.027752 0.000566 0.012734 0.008896 0.027942 0.002107 0.005284 0.008653 0.027899 0.000923 0.008307
Golgi 0.001969 0.005832 0.000167 0.006047 0.003946 0.007355 0.000115 0.003956 0.002861 0.007012 0.000155 0.004805
Lipid Particles 0.004008 0.006618 0.000347 0.001245 0.006018 0.006527 0.001135 0.000670 0.004915 0.006547 0.000530 0.000903
Mitochondria 0.002354 0.002694 0.000591 0.005052 0.003653 0.018419 0.000289 0.003881 0.002940 0.014875 0.000521 0.004356
Mitotic Spindle 0.002302 0.007380 0.004892 0.054058 0.002246 0.014599 0.005122 0.039929 0.002277 0.012972 0.004946 0.045663
None 0.003864 0.001249 0.005778 0.008707 0.004179 0.002101 0.009286 0.007000 0.004006 0.001909 0.006591 0.007693
Nuclear Periphery 0.000253 0.000655 0.001173 0.001199 0.000229 0.000459 0.001045 0.000982 0.000242 0.000503 0.001143 0.001070
Nuclear Periphery Foci 0.000448 0.001026 0.000444 0.001540 0.000420 0.000691 0.004158 0.000922 0.000435 0.000767 0.001304 0.001173
Nucleolus 0.000821 0.001022 0.000158 0.001716 0.000821 0.001349 0.000195 0.000400 0.000821 0.001275 0.000167 0.000934
Nucleus 0.124364 0.092951 0.369552* 0.155654* 0.116737 0.078573 0.254728* 0.157307* 0.120925 0.081813 0.342946* 0.156636*
Peroxisomes 0.002707 0.018426 0.000149 0.001402 0.003808 0.024156 0.000248 0.000801 0.003203 0.022864 0.000172 0.001045
Vacuole 0.052537 0.110867 0.029276 0.146517 0.051247 0.084179 0.094523 0.110291 0.051955 0.090193 0.044395 0.124992
Vacuole Periphery 0.000696 0.001610 0.000133 0.010644 0.001027 0.003533 0.000110 0.003118 0.000845 0.003100 0.000128 0.006172

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.00 -1.76 -4.44 2.57 -3.03 -5.15 -7.24 -1.12 4.70 5.60 -7.29 -2.78 -4.78 4.69 -1.58
Bud Neck -3.52 5.62 5.57 4.95 0.23 -6.02 13.41 11.20 11.08 -3.54 -7.96 11.54 12.00 12.08 -0.08
Bud Site -5.88 -2.98 -5.50 3.24 -3.27 -5.31 1.84 -1.73 3.88 -3.29 -8.72 -0.38 -5.45 4.92 -4.58
Cell Periphery -2.99 5.29 -0.83 2.61 -4.89 -0.53 2.81 10.88 7.64 8.57 -3.44 7.77 8.47 7.44 -0.35
Cytoplasm 11.77 18.20 19.15 -0.91 5.59 13.46 7.66 5.80 -8.78 -2.89 20.23 17.89 17.80 -7.20 1.37
Cytoplasmic Foci -6.05 40.71 34.21 16.95 -11.73 -6.03 42.22 41.51 33.97 -3.12 -10.93 58.47 53.87 37.64 -13.27
Eisosomes -2.31 21.54 10.20 5.27 -16.98 1.82 15.82 15.92 13.09 -0.16 -2.00 23.86 19.23 13.70 -17.17
Endoplasmic Reticulum -3.68 -9.12 -8.18 -2.28 -1.37 1.51 -3.62 1.30 -0.84 9.76 -0.01 -4.93 -1.78 -3.02 6.62
Endosome -5.79 14.20 2.31 6.42 -6.38 -8.88 11.48 9.66 12.27 -1.20 -10.60 19.15 8.31 13.30 -7.48
Golgi -2.02 5.18 -0.51 1.66 -3.17 -3.42 8.41 4.12 6.28 -2.45 -4.97 9.61 2.34 6.06 -3.99
Lipid Particles -1.08 10.32 7.74 2.39 -12.14 -0.56 9.30 10.92 9.14 8.81 -2.06 14.61 13.59 8.09 -7.08
Mitochondria -0.15 4.63 0.11 0.20 -2.24 -5.23 6.59 3.14 6.02 -2.90 -5.44 7.80 2.16 5.91 -3.35
Mitotic Spindle -1.55 -2.71 -8.36 -5.46 -7.57 -4.52 -3.77 -8.50 -2.71 -3.91 -4.83 -4.46 -11.95 -5.14 -9.60
None 6.06 -4.19 -2.68 -5.68 -0.93 3.05 -4.79 -2.83 -6.52 2.08 4.58 -5.70 -4.13 -7.86 -0.50
Nuclear Periphery -2.12 -31.12 -17.18 -4.91 0.04 -3.56 -21.70 -24.64 -14.28 1.06 -3.99 -37.66 -29.61 -14.46 0.75
Nuclear Periphery Foci -4.22 -2.86 -6.26 -1.27 -5.20 -1.84 -11.26 -3.26 -0.80 10.36 -3.35 -10.43 -7.05 -2.17 3.69
Nucleolus -0.80 6.94 -0.88 -0.22 -3.63 -1.67 2.65 2.17 4.81 -3.57 -2.12 5.61 1.32 3.24 -4.35
Nucleus 3.20 -52.54 -11.70 -10.47 30.14 7.40 -22.47 -16.72 -21.45 10.68 9.00 -57.73 -20.31 -24.12 34.72
Peroxisomes -4.33 8.99 6.29 4.96 -3.77 -8.12 12.53 11.75 9.61 -2.08 -9.33 14.90 12.54 10.74 -4.58
Vacuole -7.77 -0.82 -26.89 -12.05 -26.37 -8.05 -15.65 -27.73 -17.88 -4.41 -10.83 -10.75 -38.54 -22.97 -29.20
Vacuole Periphery -3.06 9.38 -3.42 -2.58 -4.00 -4.01 6.48 -0.05 3.51 -2.67 -4.66 10.08 -3.15 1.01 -4.76
Standard name
Human Ortholog
Description Nonfunctional homolog of Gpm1p phosphoglycerate mutase; if functional, would convert 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; GPM2 has a paralog, GPM3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (64%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gpm2

Gpm2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpm2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available