Standard name | |
---|---|
Human Ortholog | |
Description | Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 34 | 34 | 13 | 6 | 6 | 10 | 20 | 21 | 8 | 17 | 5 | 11 | – | 0 | 2 | 6 | 9 | 13 | 7 | 10 | 4 | 1 | 0 | 0 |
Bud | 16 | 12 | 4 | 1 | 4 | 3 | 8 | 5 | 3 | 9 | 4 | 2 | – | 0 | 0 | 9 | 10 | 8 | 0 | 5 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 12 | 25 | 10 | 1 | 1 | 1 |
Bud Site | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 235 | 290 | 77 | 84 | 78 | 101 | 100 | 90 | 66 | 64 | 66 | 86 | – | 17 | 47 | 285 | 353 | 332 | 272 | 298 | 98 | 16 | 9 | 26 |
Cytoplasm | 236 | 143 | 74 | 51 | 71 | 117 | 145 | 154 | 112 | 111 | 106 | 114 | – | 13 | 43 | 225 | 218 | 208 | 49 | 51 | 13 | 11 | 12 | 13 |
Endoplasmic Reticulum | 10 | 12 | 7 | 4 | 10 | 24 | 23 | 14 | 10 | 8 | 7 | 7 | – | 1 | 3 | 4 | 6 | 11 | 11 | 10 | 4 | 1 | 0 | 1 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 3 | 1 | 0 | 1 | 0 | 18 | 19 | 21 | 33 | 27 | 71 | 64 | – | 0 | 0 | 1 | 1 | 1 | 3 | 1 | 5 | 0 | 2 | 2 |
Nucleus | 0 | 4 | 3 | 0 | 2 | 3 | 3 | 1 | 6 | 4 | 3 | 2 | – | 1 | 2 | 12 | 9 | 10 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 7 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 6 | 10 | 11 | 4 | 3 | 7 | 0 | 3 | – | 0 | 0 | 3 | 8 | 8 | 2 | 4 | 1 | 1 | 0 | 0 |
Unique Cell Count | 346 | 353 | 128 | 105 | 140 | 202 | 236 | 232 | 176 | 177 | 187 | 193 | 23 | 65 | 402 | 452 | 438 | 368 | 415 | 146 | 38 | 29 | 55 | |
Labelled Cell Count | 544 | 499 | 179 | 149 | 180 | 287 | 332 | 313 | 241 | 247 | 263 | 289 | 32 | 97 | 550 | 616 | 595 | 368 | 415 | 146 | 38 | 29 | 55 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.6 | 14.0 | 9.9 | 10.1 | 10.1 | 8.6 | 8.3 | 8.4 | 7.0 | 7.1 | 6.3 | 6.7 | – | 11.8 | 12.3 | 16.4 | 16.5 | 15.4 | – | – | 13.2 |
Std Deviation (1e-4) | 1.9 | 2.7 | 1.5 | 1.4 | 1.5 | 1.5 | 1.7 | 1.5 | 1.2 | 1.5 | 1.3 | 1.4 | – | 2.1 | 2.5 | 3.5 | 3.3 | 3.3 | – | – | 2.6 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.03 | -0.21 | -0.25 | -0.24 | -0.49 | -0.48 | -0.66 | -0.57 | – | 0.25 | 0.31 | 0.73 | 0.73 | 0.64 | – | – | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.2 | -1.9 | -1.8 | -0.5 | -0.3 | -1.9 | -0.2 | -2.8 | -1.5 | – | 0 | -1.7 | 0 | 0 | -3.4 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 3.3 | -0.7 | -1.8 | -3.2 | -3.9 | -3.9 | -4.1 | -4.4 | -2.7 | – | 1.3 | 1.7 | 2.3 | 4.1 | 3.5 |
Cytoplasm | -1.4 | -1.2 | 0 | 0.7 | 1.6 | 1.0 | 0.9 | -0.2 | 0.2 | – | -0.1 | 1.1 | -0.4 | -1.9 | -2.1 |
Endoplasmic Reticulum | 0 | 0.6 | 1.9 | 1.4 | 0.2 | 0.1 | -0.4 | -0.7 | -0.8 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.5 | 3.3 | 3.5 | 5.2 | 4.6 | 7.9 | 7.3 | – | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.0367 | 9.5298 | 9.4715 | 8.5829 | 8.8513 | 8.9867 | 8.7211 | 11.1045 | 10.0488 | 9.931 | 9.3813 | 9.7398 | 9.1359 | 11.2078 | 10.1635 | 9.2047 | 8.2718 | 10.6155 |
Actin | 0.0311 | 0.0036 | 0.0098 | 0.0232 | 0.0024 | 0.0049 | 0.0221 | 0.0047 | 0.0208 | 0.0443 | 0.0316 | 0.0136 | 0.0465 | 0.0071 | 0.0175 | 0.0312 | 0.0604 | 0.0091 |
Bud | 0.0012 | 0.0102 | 0.0109 | 0.0259 | 0.0082 | 0.0064 | 0.0033 | 0.0012 | 0.0019 | 0.0045 | 0.001 | 0.0011 | 0.0014 | 0.0004 | 0.0018 | 0.0011 | 0.0008 | 0.0011 |
Bud Neck | 0.0054 | 0.0022 | 0.0056 | 0.0045 | 0.0046 | 0.0211 | 0.0032 | 0.0031 | 0.014 | 0.0171 | 0.0034 | 0.0308 | 0.0038 | 0.0016 | 0.0057 | 0.0028 | 0.0049 | 0.0326 |
Bud Periphery | 0.0069 | 0.0064 | 0.0272 | 0.0369 | 0.0179 | 0.0157 | 0.0197 | 0.0049 | 0.0158 | 0.0187 | 0.0161 | 0.0132 | 0.0122 | 0.0019 | 0.0176 | 0.0224 | 0.0069 | 0.0119 |
Bud Site | 0.0157 | 0.0761 | 0.0599 | 0.0485 | 0.0054 | 0.0054 | 0.0139 | 0.0727 | 0.0335 | 0.0145 | 0.0022 | 0.0014 | 0.0052 | 0.0578 | 0.027 | 0.0124 | 0.0041 | 0.0021 |
Cell Periphery | 0.4557 | 0.2612 | 0.4016 | 0.2891 | 0.3441 | 0.5095 | 0.5365 | 0.4513 | 0.5014 | 0.405 | 0.6037 | 0.5895 | 0.5999 | 0.5004 | 0.5864 | 0.5385 | 0.4119 | 0.6306 |
Cytoplasm | 0.3731 | 0.6299 | 0.4493 | 0.5319 | 0.545 | 0.4015 | 0.2785 | 0.4434 | 0.3554 | 0.3945 | 0.2462 | 0.3125 | 0.2094 | 0.4179 | 0.2939 | 0.3218 | 0.3422 | 0.2835 |
Cytoplasmic Foci | 0.0129 | 0.0004 | 0.0011 | 0.0013 | 0.0008 | 0.001 | 0.0083 | 0.0013 | 0.0025 | 0.0181 | 0.004 | 0.0012 | 0.0079 | 0.0004 | 0.0023 | 0.0027 | 0.0093 | 0.0004 |
Eisosomes | 0.0035 | 0.0003 | 0.0003 | 0.0013 | 0.0002 | 0.0011 | 0.0041 | 0.0004 | 0.0014 | 0.0005 | 0.0005 | 0.0008 | 0.008 | 0.0001 | 0.0011 | 0.0005 | 0.0216 | 0.0001 |
Endoplasmic Reticulum | 0.0124 | 0.0054 | 0.0075 | 0.006 | 0.0121 | 0.0164 | 0.0294 | 0.0066 | 0.0137 | 0.0257 | 0.0225 | 0.0144 | 0.0276 | 0.0064 | 0.0102 | 0.038 | 0.0186 | 0.0111 |
Endosome | 0.0144 | 0.0001 | 0.0026 | 0.0019 | 0.0155 | 0.0003 | 0.0115 | 0.0006 | 0.0053 | 0.0124 | 0.0115 | 0.004 | 0.012 | 0.0001 | 0.0028 | 0.0015 | 0.0102 | 0.0024 |
Golgi | 0.0063 | 0.0004 | 0.0029 | 0.0033 | 0.0012 | 0.0007 | 0.0079 | 0.0008 | 0.0031 | 0.0163 | 0.006 | 0.0052 | 0.0077 | 0.0005 | 0.0024 | 0.0016 | 0.0077 | 0.0011 |
Lipid Particles | 0.0083 | 0.0006 | 0.0012 | 0.0009 | 0.0011 | 0.001 | 0.011 | 0.0016 | 0.0047 | 0.0063 | 0.0072 | 0.0028 | 0.0141 | 0.0007 | 0.0049 | 0.0021 | 0.0107 | 0.0011 |
Mitochondria | 0.0026 | 0.0001 | 0.0039 | 0.0161 | 0.0038 | 0.0015 | 0.0079 | 0.0008 | 0.0038 | 0.0061 | 0.0193 | 0.0019 | 0.0036 | 0.0002 | 0.0046 | 0.0013 | 0.0057 | 0.0004 |
None | 0.0185 | 0.0004 | 0.006 | 0.0011 | 0.0029 | 0.0053 | 0.0043 | 0.0008 | 0.0034 | 0.001 | 0.0013 | 0.0003 | 0.0024 | 0.0001 | 0.0009 | 0.0017 | 0.0174 | 0.0002 |
Nuclear Periphery | 0.0052 | 0.0004 | 0.0025 | 0.0005 | 0.0062 | 0.0004 | 0.0084 | 0.0007 | 0.004 | 0.0017 | 0.006 | 0.001 | 0.0079 | 0.0005 | 0.0024 | 0.0022 | 0.0125 | 0.0056 |
Nucleolus | 0.0009 | 0.0001 | 0.0004 | 0.0003 | 0.0017 | 0.0004 | 0.0009 | 0.0003 | 0.0006 | 0.0003 | 0.0004 | 0.0002 | 0.0006 | 0 | 0.0004 | 0.0005 | 0.0023 | 0.0001 |
Nucleus | 0.0061 | 0.0004 | 0.0018 | 0.001 | 0.0118 | 0.0009 | 0.0083 | 0.0006 | 0.0043 | 0.0013 | 0.0011 | 0.0005 | 0.0023 | 0.0002 | 0.0007 | 0.0031 | 0.0039 | 0.0005 |
Peroxisomes | 0.0111 | 0 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0065 | 0.0018 | 0.0024 | 0.0054 | 0.0015 | 0.0007 | 0.0155 | 0.0014 | 0.0099 | 0.0017 | 0.0051 | 0.0002 |
Punctate Nuclear | 0.0016 | 0 | 0.0005 | 0.0001 | 0.0007 | 0.0002 | 0.003 | 0.0001 | 0.0008 | 0.0011 | 0.0023 | 0.0004 | 0.0051 | 0 | 0.0019 | 0.0041 | 0.0404 | 0.0004 |
Vacuole | 0.0066 | 0.0017 | 0.0041 | 0.0056 | 0.0126 | 0.0061 | 0.01 | 0.0025 | 0.0066 | 0.0048 | 0.0105 | 0.0041 | 0.0064 | 0.0025 | 0.0056 | 0.0086 | 0.0026 | 0.0053 |
Vacuole Periphery | 0.0007 | 0 | 0.0003 | 0.0003 | 0.0019 | 0.0001 | 0.0014 | 0 | 0.0005 | 0.0005 | 0.0017 | 0.0004 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.6223 | 7.9418 | 5.8932 | 17.5861 | 11.9026 | 17.7584 | 17.3983 | 22.0401 | 20.2748 | 20.7192 |
Translational Efficiency | 0.8098 | 1.1006 | 1.5447 | 0.6407 | 0.7522 | 0.9593 | 0.7634 | 0.5856 | 0.9008 | 0.8392 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1803 | 1084 | 1047 | 1801 | 1992 | 1938 | 242 | 757 | 3795 | 3022 | 1289 | 2558 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 868.20 | 1119.08 | 1264.18 | 1009.10 | 973.47 | 1089.64 | 1164.93 | 964.70 | 923.46 | 1100.20 | 1245.55 | 995.96 |
Standard Deviation | 114.38 | 194.47 | 219.29 | 149.02 | 143.71 | 181.41 | 148.11 | 183.24 | 140.78 | 186.74 | 211.38 | 161.19 |
Intensity Change Log 2 | — | 0.366214 | 0.542103 | 0.216970 | — | 0.162643 | 0.259035 | -0.013056 | — | 0.262196 | 0.399413 | 0.099961 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003454 | 0.005976 | 0.034054 | 0.038383 | 0.005592 | 0.009112 | 0.051453 | 0.026793 | 0.004577 | 0.007987 | 0.037321 | 0.034953 |
Bud Neck | 0.058119 | 0.011741 | 0.031585 | 0.033750 | 0.023145 | 0.016182 | 0.030980 | 0.016369 | 0.039761 | 0.014589 | 0.031471 | 0.028606 |
Bud Site | 0.071842 | 0.140939* | 0.227772* | 0.145406* | 0.120137* | 0.128029* | 0.191591* | 0.140220* | 0.097192 | 0.132660* | 0.220979* | 0.143871* |
Cell Periphery | 0.082157 | 0.134187* | 0.067943 | 0.031496 | 0.131081* | 0.134241* | 0.071280 | 0.019382 | 0.107837* | 0.134222* | 0.068570 | 0.027911 |
Cytoplasm | 0.702450* | 0.671988* | 0.434413* | 0.492545* | 0.694671* | 0.681075* | 0.240574* | 0.460346* | 0.698367* | 0.677815* | 0.398021* | 0.483016* |
Cytoplasmic Foci | 0.015515 | 0.012264 | 0.023300 | 0.010050 | 0.006864 | 0.007264 | 0.040410 | 0.032202 | 0.010974 | 0.009057 | 0.026513 | 0.016605 |
Eisosomes | 0.011617 | 0.000410 | 0.003261 | 0.009633 | 0.001388 | 0.000867 | 0.013428 | 0.004336 | 0.006248 | 0.000703 | 0.005169 | 0.008065 |
Endoplasmic Reticulum | 0.003484 | 0.002197 | 0.009715 | 0.004160 | 0.002224 | 0.002206 | 0.023786 | 0.002866 | 0.002823 | 0.002203 | 0.012357 | 0.003777 |
Endosome | 0.001488 | 0.002039 | 0.001775 | 0.001943 | 0.000574 | 0.001827 | 0.003539 | 0.009296 | 0.001008 | 0.001903 | 0.002106 | 0.004119 |
Golgi | 0.003482 | 0.003100 | 0.001458 | 0.001537 | 0.001474 | 0.002506 | 0.001026 | 0.005206 | 0.002428 | 0.002719 | 0.001377 | 0.002623 |
Lipid Particles | 0.002606 | 0.001354 | 0.013431 | 0.002032 | 0.000573 | 0.001644 | 0.007115 | 0.003404 | 0.001539 | 0.001540 | 0.012245 | 0.002438 |
Mitochondria | 0.001215 | 0.000334 | 0.000124 | 0.001056 | 0.001289 | 0.001088 | 0.000045 | 0.001452 | 0.001254 | 0.000817 | 0.000110 | 0.001173 |
Mitotic Spindle | 0.000764 | 0.000186 | 0.000238 | 0.003798 | 0.000161 | 0.000940 | 0.000224 | 0.010643 | 0.000448 | 0.000670 | 0.000235 | 0.005824 |
None | 0.001582 | 0.000144 | 0.000387 | 0.000734 | 0.000121 | 0.000093 | 0.000067 | 0.002455 | 0.000815 | 0.000112 | 0.000327 | 0.001243 |
Nuclear Periphery | 0.000074 | 0.000047 | 0.000114 | 0.000115 | 0.000043 | 0.000055 | 0.000132 | 0.000605 | 0.000058 | 0.000052 | 0.000118 | 0.000260 |
Nuclear Periphery Foci | 0.000648 | 0.000672 | 0.004823 | 0.001222 | 0.000238 | 0.000421 | 0.007326 | 0.002267 | 0.000433 | 0.000511 | 0.005293 | 0.001531 |
Nucleolus | 0.000126 | 0.000029 | 0.000042 | 0.000053 | 0.000037 | 0.000068 | 0.000029 | 0.000138 | 0.000079 | 0.000054 | 0.000039 | 0.000078 |
Nucleus | 0.012842 | 0.001047 | 0.030402 | 0.065781 | 0.001885 | 0.001550 | 0.028994 | 0.032587 | 0.007091 | 0.001370 | 0.030138 | 0.055958 |
Peroxisomes | 0.001998 | 0.001154 | 0.000838 | 0.000958 | 0.001242 | 0.001435 | 0.000823 | 0.003987 | 0.001601 | 0.001334 | 0.000835 | 0.001854 |
Vacuole | 0.023754 | 0.009237 | 0.114156 | 0.154520 | 0.006703 | 0.008322 | 0.287103* | 0.222813* | 0.014804 | 0.008650 | 0.146626 | 0.174730 |
Vacuole Periphery | 0.000781 | 0.000955 | 0.000168 | 0.000830 | 0.000557 | 0.001076 | 0.000076 | 0.002635 | 0.000663 | 0.001032 | 0.000151 | 0.001364 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.33 | -17.86 | -17.91 | -16.12 | -0.96 | -4.01 | -9.39 | -7.36 | -5.52 | 5.00 | -5.88 | -19.53 | -18.80 | -15.39 | 1.99 |
Bud Neck | 17.31 | 9.61 | 10.06 | -10.37 | 0.06 | 4.26 | -1.22 | 5.79 | 2.14 | 4.20 | 16.02 | 5.25 | 8.68 | -7.90 | 2.57 |
Bud Site | -10.79 | -19.94 | -12.40 | 1.07 | 10.45 | -1.39 | -4.50 | 0.68 | 1.67 | 4.50 | -8.51 | -17.45 | -7.13 | 0.98 | 11.70 |
Cell Periphery | -7.96 | 5.02 | 18.35 | 17.52 | 10.23 | -0.52 | 8.60 | 25.94* | 25.16* | 6.57 | -6.07 | 12.16 | 30.51 | 29.89* | 12.61 |
Cytoplasm | 3.31 | 29.73 | 28.78 | 19.40 | -5.08 | 1.66 | 32.88 | 21.15 | 19.61 | -12.34 | 3.40 | 38.06 | 35.52 | 29.27 | -8.48 |
Cytoplasmic Foci | 2.01 | -6.59 | 3.78 | 0.79 | 10.43 | -0.42 | -9.43 | -11.22 | -10.84 | 2.83 | 2.22 | -13.29 | -6.32 | -7.78 | 8.12 |
Eisosomes | 23.72* | 17.13 | 4.86 | -15.38 | -9.72 | 4.81 | -7.79 | -4.16 | -5.36 | 6.05 | 22.47 | 4.03 | -1.17 | -15.73 | -4.29 |
Endoplasmic Reticulum | 1.29 | -5.09 | -0.73 | -2.15 | 5.16 | 0.04 | -7.37 | -0.49 | -0.62 | 7.30 | 1.24 | -8.88 | -1.57 | -3.10 | 8.35 |
Endosome | -1.16 | -1.72 | -0.53 | 0.45 | 0.14 | -2.85 | -8.04 | -4.62 | -3.57 | -2.44 | -2.70 | -7.45 | -4.31 | -2.28 | -2.16 |
Golgi | 0.48 | 9.75 | 3.84 | 1.93 | 0.17 | -1.99 | 3.07 | -1.56 | -0.78 | -1.97 | -0.67 | 7.44 | 0.78 | 1.06 | -1.22 |
Lipid Particles | 5.47 | -10.58 | 2.98 | -3.47 | 11.17 | -3.38 | -9.96 | -4.65 | -2.37 | 4.34 | -0.04 | -12.81 | -4.42 | -3.06 | 11.49 |
Mitochondria | 6.64 | 8.94 | 0.77 | -1.30 | -1.80 | 0.49 | 3.41 | 0.51 | 0.12 | -3.30 | 1.89 | 5.70 | 0.89 | -0.34 | -2.66 |
Mitotic Spindle | 1.66 | 1.37 | -2.28 | -2.99 | -2.89 | -1.41 | -0.78 | -3.48 | -3.06 | -3.45 | -0.60 | 1.02 | -4.25 | -3.82 | -4.48 |
None | 5.77 | 4.42 | 2.99 | -3.34 | -1.39 | 1.47 | 2.43 | -1.64 | -1.66 | -1.68 | 5.92 | 3.33 | -0.67 | -2.76 | -1.94 |
Nuclear Periphery | 4.06 | -6.98 | -2.21 | -3.31 | 0.60 | -1.06 | -7.04 | -1.39 | -1.35 | -1.07 | 0.85 | -9.11 | -1.83 | -1.90 | -0.94 |
Nuclear Periphery Foci | -0.38 | -15.71 | -2.85 | -2.58 | 10.65 | -2.26 | -8.95 | -5.43 | -4.75 | 6.07 | -1.36 | -18.53 | -5.85 | -5.17 | 12.06 |
Nucleolus | 11.45 | 12.46 | 7.04 | -2.48 | -1.11 | -0.90 | 3.12 | -3.37 | -1.03 | -3.74 | 1.01 | 10.49 | 0.80 | -0.65 | -3.35 |
Nucleus | 17.91 | -10.43 | -23.86 | -30.33 | -8.16 | 2.36 | -12.47 | -15.67 | -15.83 | -1.24 | 17.08 | -14.60 | -29.32 | -33.28 | -7.12 |
Peroxisomes | 6.03 | 9.54 | 6.30 | 1.70 | -0.87 | -1.43 | 4.83 | -2.21 | -1.96 | -2.71 | 2.52 | 11.06 | 0.15 | -0.81 | -2.84 |
Vacuole | 12.00 | -21.90 | -34.41 | -37.96 | -9.17 | -2.98 | -20.12 | -28.66 | -28.48 | 1.82 | 9.45 | -28.55 | -45.11 | -46.66 | -7.81 |
Vacuole Periphery | -0.40 | 2.05 | 0.21 | 0.56 | -1.32 | -1.27 | 10.43 | -1.44 | -0.79 | -1.98 | -1.16 | 3.55 | -0.88 | 0.02 | -2.30 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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