Standard name
Human Ortholog
Description Sterol transport protein and functional homolog of human NPC2/He1; human NPC2 is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes; yeast NPC2 can complement mutations in human NPC2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.07 0 0.2 0.14 0.11 0.15 0.11 0.15 0.17 0.2 0.26 0.26 0.53 0.69 0.14 0.1 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0.06 0 0 0 0 0 0 0 0 0 0 0 0.18 0 0.08 0.05 0.08 0 0.05 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.13 0 0 0 0.15 0 0.06 0.05 0 0 0.05 0 0 0 0.08 0.05 0 0 0 0 0 0 0
Nucleus 0.26 0.18 0.46 0.41 0.26 0.34 0.32 0.35 0.33 0.27 0.25 0.22 0.06 0.05 0 0.4 0.38 0.38 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.66 0.78 0.63 0.73 0.74 0.61 0.7 0.67 0.7 0.72 0.73 0.71 0.73 0.57 0.36 0.48 0.59 0.66 0.87 0.89 0.92 0.91 0.88 0.87
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 2 0 0 1 2 0 0 1 0 0 0 0 1 0 2 4 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 18 3 35 42 31 65 71 54 69 59 74 53 216 355 52 36 33 1 5 1 2 9 7
Endoplasmic Reticulum 1 0 2 1 2 0 0 0 0 0 0 0 2 1 0 21 14 18 0 0 0 0 0 1
Endosome 9 6 0 3 13 2 10 5 1 4 0 1 37 18 42 20 27 14 8 7 0 12 23 28
Golgi 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 1 3
Mitochondria 26 32 3 2 3 42 3 36 17 16 13 14 9 5 2 30 18 9 4 2 3 2 6 3
Nucleus 41 44 31 72 80 98 139 219 119 109 73 62 12 20 12 146 133 171 1 6 0 2 2 5
Nuclear Periphery 2 2 0 1 1 1 1 0 1 4 0 0 2 1 0 0 1 2 1 0 0 1 8 5
Nucleolus 4 2 0 0 1 0 1 1 1 2 2 1 3 1 1 0 2 1 1 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 2 1 2 0 1 1 1 0 1 1 0 7 0 2 1 1 0 0 0 2 2
Vac/Vac Membrane 104 187 42 129 225 173 308 423 254 291 210 201 151 230 188 174 206 294 151 231 96 273 466 445
Unique Cell Count 157 240 67 176 305 285 440 629 365 404 289 284 206 405 518 365 347 446 174 260 104 300 532 513
Labelled Cell Count 195 292 82 245 369 350 528 758 448 497 358 356 270 492 608 444 440 544 174 260 104 300 532 513


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.7 5.1 6.4 8.8 6.6 9.1 8.3 8.5 8.6 7.8 8.1 5.5 5.2 5.1 6.6 7.4 7.6 5.2 5.2 5.4
Std Deviation (1e-4) 1.0 1.3 0.9 1.6 2.1 2.3 2.7 2.5 2.5 2.5 2.3 2.2 1.3 1.2 1.1 1.8 2.2 2.0 1.0 1.4 1.6
Intensity Change (Log2) – – – 0.34 0.79 0.39 0.84 0.71 0.74 0.77 0.63 0.68 0.11 0.03 0.02 0.38 0.56 0.59 0.03 0.04 0.1

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.0 2.1 1.6 2.3 1.7 2.3 2.7 3.1 3.8 3.7 7.4 10.1 2.2 1.5 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 3.7 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.3 0 0 0 0 0 0 0 0 0 1.1 0 0
Nucleus -0.8 -3.2 -1.8 -2.4 -1.9 -2.2 -3.2 -3.4 -4.1 -7.9 -10.1 0 -1.0 -1.2 -1.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.6 1.8 -0.3 1.2 0.8 1.1 1.6 1.6 1.3 1.7 -0.9 -4.2 -2.3 -0.5 0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0086 1.4767 0.9308 0.5884 0.7412 1.0855 1.3598 3.2148 2.1593 2.0988 2.22 2.4676 2.8041 4.1557 3.7402 3.3466 3.434 3.7337
Actin 0.0015 0 0 0 0 0 0.0046 0 0.0004 0.002 0.0045 0.0003 0.021 0.0001 0.0046 0.0013 0.0247 0.0045
Bud 0.0003 0.0005 0.0003 0.0003 0.0003 0.0003 0.0008 0.0006 0.0003 0.0009 0.0005 0.0003 0.0006 0.0002 0.0007 0.0004 0.0009 0.0004
Bud Neck 0.0008 0.0017 0.001 0.001 0.0014 0.0012 0.0017 0.0011 0.0009 0.0012 0.0014 0.0019 0.0015 0.0012 0.0013 0.0074 0.0013 0.002
Bud Periphery 0.0011 0.0012 0.0009 0.0006 0.0009 0.0012 0.0024 0.001 0.0009 0.0019 0.0012 0.0012 0.002 0.0008 0.0017 0.0014 0.0025 0.0014
Bud Site 0.0007 0.0017 0.0013 0.0016 0.0012 0.0005 0.0017 0.0032 0.001 0.0012 0.0014 0.0007 0.0013 0.0009 0.0045 0.0013 0.0013 0.001
Cell Periphery 0.0023 0.0027 0.0027 0.0018 0.0023 0.0041 0.0026 0.0026 0.0023 0.0027 0.0018 0.0035 0.0022 0.0042 0.0036 0.0019 0.0015 0.0045
Cytoplasm 0.0044 0.007 0.0044 0.0017 0.0016 0.0027 0.0032 0.0018 0.0024 0.001 0.0009 0.0012 0.0066 0.0055 0.0082 0.0009 0.0017 0.0018
Cytoplasmic Foci 0.0096 0.009 0.0092 0.0108 0.004 0.0059 0.0082 0.0035 0.0053 0.0038 0.0059 0.0047 0.0158 0.0035 0.0133 0.0038 0.0035 0.0054
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0.0002 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0024 0.0019 0.0022 0.0008 0.0009 0.0022 0.0043 0.0032 0.003 0.0006 0.001 0.0015 0.0049 0.0055 0.0026 0.0019 0.0023 0.0021
Endosome 0.2104 0.1173 0.1827 0.2143 0.1488 0.1157 0.2176 0.1254 0.1994 0.1771 0.1635 0.1214 0.2337 0.1139 0.2113 0.2141 0.1771 0.1349
Golgi 0.0054 0.0024 0.0053 0.0076 0.0044 0.0023 0.0095 0.0027 0.0098 0.0074 0.0068 0.0045 0.0111 0.0032 0.0148 0.0113 0.0076 0.0061
Lipid Particles 0.0323 0.0068 0.0369 0.006 0.0127 0.0155 0.0403 0.008 0.0221 0.0055 0.0191 0.0199 0.0326 0.0103 0.03 0.0127 0.0055 0.0161
Mitochondria 0.0218 0.0094 0.0287 0.0056 0.0157 0.0123 0.0316 0.008 0.0382 0.0548 0.0172 0.0209 0.0139 0.0098 0.0476 0.0444 0.0036 0.0184
None 0.0027 0.0006 0.0045 0.0001 0.0004 0.0037 0.0037 0.0002 0.0008 0.0002 0.0002 0.0002 0.0054 0.005 0.0077 0.0002 0.0012 0.0003
Nuclear Periphery 0.0056 0.0033 0.0148 0.0013 0.0016 0.0055 0.0188 0.0085 0.0125 0.0012 0.0022 0.0077 0.0075 0.0079 0.004 0.0037 0.0049 0.0056
Nucleolus 0.008 0.0069 0.0179 0.002 0.0137 0.004 0.0079 0.006 0.0041 0.0029 0.0038 0.0098 0.0038 0.0038 0.0033 0.0041 0.003 0.0103
Nucleus 0.0135 0.0047 0.0195 0.0019 0.0062 0.006 0.0145 0.0071 0.0037 0.0022 0.0036 0.0076 0.0104 0.0057 0.0027 0.0029 0.0034 0.0128
Peroxisomes 0.0021 0.0011 0.0022 0.0012 0.0012 0.0008 0.0069 0.0005 0.0016 0.0015 0.01 0.0026 0.0078 0.0007 0.0045 0.0015 0.0012 0.0019
Punctate Nuclear 0.0007 0.0001 0.0014 0.0001 0.0009 0.0003 0.0016 0.0002 0.0004 0.0001 0.0004 0.0015 0.0062 0.0009 0.0004 0.0006 0.0006 0.0014
Vacuole 0.48 0.6529 0.4947 0.5732 0.6691 0.6317 0.4447 0.6402 0.4919 0.6051 0.6208 0.61 0.4447 0.6466 0.459 0.5109 0.6352 0.6108
Vacuole Periphery 0.1944 0.1691 0.1693 0.1681 0.113 0.1843 0.1733 0.1762 0.1991 0.1267 0.1337 0.1785 0.1668 0.1701 0.1739 0.1733 0.1168 0.1583

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 114.1453 149.2792 103.7607 136.7326 104.1442 118.3074 150.4584 146.3599 160.5382 132.2715
Translational Efficiency 1.9224 1.2303 2.063 1.5869 1.532 2.099 1.4556 1.7203 1.8086 1.6372

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
739 256 444 125 2083 2366 148 1512 2822 2622 592 1637

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 754.72 841.04 907.06 838.14 748.45 811.28 910.86 862.17 750.09 814.19 908.01 860.34
Standard Deviation 105.56 122.35 106.22 95.37 89.80 99.42 113.41 107.80 94.22 102.27 108.07 107.10
Intensity Change Log 2 — 0.156233 0.265256 0.151250 — 0.116294 0.283323 0.204066 — 0.136485 0.274281 0.177790

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000028 0.000031 0.000286 0.000081 0.000025 0.000021 0.000387 0.000232 0.000025 0.000022 0.000311 0.000221
Bud Neck 0.014068 0.020868 0.015645 0.022212 0.011117 0.015251 0.006569 0.015538 0.011889 0.015799 0.013376 0.016048
Bud Site 0.005596 0.014927 0.014293 0.004544 0.005149 0.005757 0.009455 0.011416 0.005266 0.006652 0.013083 0.010892
Cell Periphery 0.001627 0.000370 0.000094 0.000260 0.000352 0.000998 0.000541 0.000072 0.000686 0.000937 0.000206 0.000086
Cytoplasm 0.011312 0.043984 0.233622 0.166851 0.042558 0.012438 0.182326 0.458542 0.034376 0.015518 0.220798 0.436268
Cytoplasmic Foci 0.006719 0.021894 0.061634 0.080812 0.012245 0.004052 0.052059 0.062355 0.010798 0.005794 0.059241 0.063764
Eisosomes 0.000017 0.000017 0.000059 0.000243 0.000019 0.000020 0.000575 0.000034 0.000019 0.000020 0.000188 0.000050
Endoplasmic Reticulum 0.002723 0.001250 0.003836 0.001024 0.003070 0.005552 0.005727 0.001523 0.002979 0.005132 0.004308 0.001485
Endosome 0.050422 0.070474 0.082856 0.028533 0.033031 0.034930 0.046464 0.056151 0.037585 0.038401 0.073758 0.054042
Golgi 0.000645 0.000570 0.000547 0.000316 0.000517 0.000883 0.000683 0.001731 0.000550 0.000853 0.000581 0.001623
Lipid Particles 0.001591 0.000510 0.005030 0.005265 0.001109 0.001241 0.006303 0.002258 0.001235 0.001169 0.005349 0.002488
Mitochondria 0.001216 0.000518 0.000629 0.000450 0.001330 0.002489 0.000682 0.000778 0.001300 0.002297 0.000643 0.000753
Mitotic Spindle 0.000132 0.000111 0.018412 0.002037 0.001058 0.000589 0.002034 0.008534 0.000815 0.000542 0.014317 0.008038
None 0.000475 0.000071 0.002028 0.003216 0.000292 0.000280 0.004199 0.001824 0.000340 0.000260 0.002571 0.001930
Nuclear Periphery 0.000141 0.000111 0.003283 0.000872 0.000271 0.000619 0.004920 0.001538 0.000237 0.000570 0.003693 0.001487
Nuclear Periphery Foci 0.000211 0.000180 0.007919 0.000978 0.000080 0.000076 0.007007 0.001937 0.000114 0.000087 0.007691 0.001864
Nucleolus 0.000445 0.000941 0.007079 0.011262 0.000505 0.001113 0.002059 0.001986 0.000490 0.001096 0.005824 0.002694
Nucleus 0.005146 0.006697 0.158225 0.445559 0.015637 0.021755 0.413658 0.116647 0.012889 0.020285 0.222083 0.141762
Peroxisomes 0.001104 0.001081 0.007435 0.004726 0.000492 0.000531 0.003684 0.002806 0.000652 0.000585 0.006497 0.002953
Vacuole 0.879343 0.791931 0.373228 0.219802 0.862683 0.870494 0.247392 0.252413 0.867045 0.862824 0.341769 0.249923
Vacuole Periphery 0.017040 0.023462 0.003861 0.000960 0.008460 0.020908 0.003275 0.001685 0.010707 0.021158 0.003714 0.001630

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.40 -6.38 -1.96 -1.89 3.20 0.23 -6.58 -2.08 -2.13 3.04 0.26 -8.83 -2.16 -2.21 1.96
Bud Neck -1.44 -0.99 -0.79 0.18 -0.39 -2.88 3.25 -2.85 -0.35 -5.13 -2.80 -1.38 -2.61 -0.21 -1.00
Bud Site -3.85 -3.31 0.17 3.04 2.60 -0.59 -2.47 -3.92 -3.58 0.03 -1.54 -4.08 -3.95 -2.67 1.48
Cell Periphery 6.72 8.76 7.81 1.62 -5.19 -3.95 -0.39 11.70 5.76 3.74 -1.63 9.40 12.04 5.85 3.83
Cytoplasm -4.31 -19.14 -9.15 -6.50 3.34 10.04 -8.82 -48.41 -54.49 -13.57 7.36 -19.34 -49.52 -53.02 -15.62
Cytoplasmic Foci -3.75 -12.11 -6.29 -4.52 -1.18 7.94 -5.79 -18.39 -22.68 -2.78 5.30 -12.84 -20.07 -22.57 -1.41
Eisosomes -0.19 -10.39 -8.70 -8.65 -6.75 -0.35 -2.49 -7.64 -7.85 2.38 -0.63 -3.34 -10.83 -10.50 2.51
Endoplasmic Reticulum 4.07 -3.36 3.51 -0.48 7.19 -5.97 -3.53 6.30 10.98 4.87 -5.87 -4.70 7.16 10.84 8.83
Endosome -2.14 -2.78 3.03 4.22 5.11 -0.84 -0.47 -4.45 -3.81 -1.40 -0.32 -4.89 -2.75 -2.49 2.74
Golgi 0.26 0.50 1.05 1.60 1.48 -1.46 -0.17 -1.19 -0.63 -1.08 -1.31 0.25 -1.11 -0.61 -1.19
Lipid Particles 1.30 -3.45 -2.59 -4.86 0.41 -0.44 -6.50 -7.45 -3.68 4.68 0.26 -7.64 -4.61 -4.87 5.68
Mitochondria 1.14 0.94 1.31 0.60 1.69 -1.84 1.50 1.22 4.22 -0.76 -1.85 1.72 1.50 4.21 -0.61
Mitotic Spindle 0.27 -4.49 -1.41 -1.41 2.37 0.85 -1.66 -4.84 -5.17 -3.54 0.62 -4.45 -5.19 -5.37 2.22
None 0.98 -2.00 -2.28 -2.91 -0.80 0.08 -2.34 -4.67 -5.08 1.02 0.51 -3.35 -4.86 -5.69 0.59
Nuclear Periphery 0.65 -9.20 -5.65 -5.84 6.50 -1.84 -1.79 -13.49 -6.00 0.96 -1.95 -6.57 -14.40 -6.54 3.80
Nuclear Periphery Foci -0.03 -7.18 -3.61 -3.65 5.66 0.26 -5.61 -9.61 -9.62 3.96 0.86 -8.70 -9.90 -10.05 6.52
Nucleolus -1.54 -4.58 -2.41 -2.28 -0.39 -4.85 -4.36 -8.26 -5.41 -2.24 -5.15 -4.80 -6.99 -5.22 2.60
Nucleus -0.92 -15.85 -14.34 -14.27 -8.12 -2.57 -12.17 -24.22 -21.82 8.00 -3.59 -18.61 -26.77 -24.27 5.53
Peroxisomes 0.19 -3.41 -1.53 -1.59 0.92 -0.29 -3.77 -5.78 -5.72 0.87 0.59 -4.42 -5.53 -5.88 2.31
Vacuole 5.21 34.40 22.30 17.06 3.30 -1.30 21.08 74.34 78.39 2.59 0.69 42.21 80.27 79.27 6.52
Vacuole Periphery -2.31 9.97 11.31 9.48 3.33 -10.87 5.77 14.59 17.99 0.62 -9.53 12.76 17.84 19.65 3.07
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Sterol transport protein and functional homolog of human NPC2/He1; human NPC2 is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes; yeast NPC2 can complement mutations in human NPC2
Localization
Cell Percentages vacuole (94%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Npc2

Npc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Npc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available