Standard name
Human Ortholog
Description Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.73 0.75 0.62 0.32 0.24 0.35 0.38 – 0.29 0.32 0.32 0.28 0.74 0.65 0.44 0.48 0.6 0.59 0.66 0.49 0.49 0.82 0.79 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0.05 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.34 0.07 – 0.41 0.43 0.49 0.57 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0.14 0.15 0.17 0.2 0.26 – 0.29 0.24 0.21 0.23 0 0.07 0.15 0.08 0.08 0.07 0.2 0.3 0.3 0.09 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.39 0.41 0.56 0.58 0.65 0.32 0.38 – 0.15 0.18 0.14 0.14 0.43 0.51 0.54 0.58 0.49 0.45 0.05 0.13 0.1 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 0 3 1 – 2 5 1 2 0 0 0 0 0 0 1 10 2 0 3 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 2 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 3 0 3 – 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0
Cytoplasm 258 452 77 45 42 41 93 – 47 65 30 39 237 313 205 96 80 89 247 333 72 247 460 408
Endoplasmic Reticulum 3 6 2 3 0 0 0 – 1 0 0 0 3 4 1 5 4 4 3 4 3 4 16 7
Endosome 6 0 0 3 9 6 10 – 2 5 5 1 1 1 5 5 1 1 10 19 3 2 29 22
Golgi 1 1 0 0 0 0 0 – 0 0 0 0 3 0 14 6 0 6 2 1 1 0 4 7
Mitochondria 0 1 0 1 2 39 18 – 67 88 47 79 0 0 0 0 2 1 0 0 0 1 0 2
Nucleus 5 26 17 22 29 23 63 – 47 50 20 32 6 32 68 16 11 11 74 206 44 26 21 13
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 0 2 0 0 0 0 0 0 5 7 1 3 7 3
Nucleolus 0 0 1 0 0 0 0 – 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 1 – 0 1 0 1 0 0 0 0 0 0 2 6 1 0 6 8
Vac/Vac Membrane 139 245 70 83 114 37 95 – 25 37 13 19 138 245 255 116 66 67 18 87 14 12 20 17
Unique Cell Count 355 603 124 142 175 116 247 164 206 95 138 321 478 468 200 134 150 374 685 149 303 585 501
Labelled Cell Count 414 731 168 157 199 149 284 192 254 117 176 388 597 549 244 165 179 374 685 149 303 585 501


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 12.0 10.5 10.3 9.9 5.9 6.8 – 5.7 5.7 5.5 5.3 13.8 11.7 13.0 14.3 13.6 13.2 11.5 12.9 13.1
Std Deviation (1e-4) 1.7 1.6 1.5 1.3 1.7 1.8 1.6 – 1.9 1.6 1.9 1.8 2.1 1.8 2.1 2.6 3.0 2.3 1.8 1.8 2.2
Intensity Change (Log2) – – – -0.04 -0.09 -0.83 -0.63 – -0.89 -0.88 -0.95 -0.99 0.39 0.15 0.3 0.43 0.37 0.32 0.13 0.29 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -5.0 -6.6 -4.1 -4.5 – -5.7 -5.4 -4.5 -5.5 2.4 0.7 -3.6 -2.5 -0.4 -0.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 7.1 3.1 – 8.1 8.5 8.8 10.1 0 0 0 0 0 0
Nucleus 0.4 0.7 1.3 2.6 – 3.0 2.3 1.4 2.0 -5.1 -2.5 0.2 -1.7 -1.4 -1.7
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0.3 1.5 -3.8 -3.3 – -7.4 -7.2 -6.5 -7.3 -2.6 -1.0 -0.4 0.3 -1.2 -1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.5615 6.7627 6.0202 5.4444 5.4483 6.0134 6.9495 8.172 7.8002 7.2438 6.7866 7.7314 10.113 11.7591 10.421 10.5743 10.5836 10.8587
Actin 0.029 0.0062 0.0145 0.0034 0.0017 0.0006 0.0189 0.0006 0.0128 0.0003 0.0145 0.0001 0.0006 0.0004 0.0004 0.0001 0.0001 0.0002
Bud 0.0005 0.0002 0.0005 0.0002 0.0004 0.0002 0.0004 0.0003 0.0002 0.0001 0.0003 0.0001 0.0002 0.0006 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0019 0.0008 0.0044 0.001 0.0014 0.0029 0.0025 0.0027 0.0014 0.0009 0.0013 0.0017 0.0005 0.0008 0.0004 0.0006 0.0006 0.0015
Bud Periphery 0.0005 0.0001 0.0004 0.0001 0.0002 0.0001 0.0004 0.0002 0.0001 0 0.0003 0 0.0001 0.0001 0 0 0 0
Bud Site 0.0009 0.0025 0.0024 0.0005 0.0008 0.0002 0.0014 0.0022 0.0008 0.0002 0.0004 0.0001 0.0003 0.0011 0.0002 0.0001 0.0001 0.0001
Cell Periphery 0.0001 0 0.0001 0.0001 0 0 0.0002 0 0 0 0.0001 0 0 0 0 0 0 0
Cytoplasm 0.4365 0.4374 0.4335 0.2505 0.5276 0.3555 0.4068 0.387 0.3549 0.3066 0.4493 0.2784 0.6196 0.5571 0.5177 0.5642 0.5449 0.4797
Cytoplasmic Foci 0.0095 0.0043 0.0111 0.0266 0.0093 0.0021 0.0066 0.0018 0.0018 0.0016 0.0065 0.0013 0.0022 0.0012 0.0014 0.0011 0.0009 0.0012
Eisosomes 0.0002 0 0 0.0001 0 0 0.0007 0 0.0001 0 0.0002 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0106 0.0022 0.0029 0.0183 0.005 0.0038 0.007 0.0015 0.0034 0.0032 0.0064 0.0018 0.0062 0.0027 0.0023 0.001 0.0064 0.0033
Endosome 0.026 0.0032 0.0122 0.0129 0.0269 0.0066 0.0104 0.0018 0.0059 0.0032 0.0224 0.0017 0.0036 0.0009 0.0009 0.0008 0.0021 0.0022
Golgi 0.0063 0.0002 0.003 0.0016 0.0047 0.0005 0.0034 0.0002 0.001 0.0001 0.0032 0 0.0001 0.0001 0.0001 0 0 0
Lipid Particles 0.0094 0.0001 0.0025 0.0042 0.0033 0.0005 0.0034 0 0.0003 0 0.0051 0 0.0001 0 0 0 0 0
Mitochondria 0.0108 0.0003 0.0045 0.0016 0.0023 0.005 0.0034 0.0003 0.0008 0.0004 0.0064 0.0002 0.0005 0.0005 0.0003 0.0002 0.0002 0.0004
None 0.0047 0.0011 0.0023 0.0061 0.002 0.0012 0.0046 0.0014 0.0015 0.0027 0.0037 0.0009 0.0032 0.0021 0.0038 0.0007 0.0009 0.0008
Nuclear Periphery 0.0317 0.016 0.0171 0.0135 0.0205 0.02 0.0291 0.0142 0.019 0.0192 0.0421 0.0136 0.0222 0.0161 0.0111 0.0082 0.0211 0.0167
Nucleolus 0.0007 0.0004 0.0013 0.0007 0.0005 0.0005 0.001 0.0004 0.0006 0.0004 0.0008 0.0005 0.0002 0.0003 0.0002 0.0001 0.0002 0.0002
Nucleus 0.3987 0.5174 0.4724 0.6151 0.3799 0.5921 0.485 0.5802 0.5883 0.6521 0.4148 0.693 0.3358 0.4109 0.4578 0.419 0.4178 0.488
Peroxisomes 0.0108 0.0003 0.0028 0.0019 0.0021 0.0002 0.0055 0.0002 0.0003 0.0001 0.005 0 0.0001 0.0001 0.0001 0 0 0
Punctate Nuclear 0.0065 0.0054 0.0078 0.0403 0.0068 0.0052 0.0067 0.0034 0.005 0.0078 0.0142 0.0052 0.0028 0.0035 0.0024 0.0013 0.0032 0.0037
Vacuole 0.0033 0.0015 0.0037 0.0011 0.0036 0.0021 0.0021 0.0013 0.0014 0.0009 0.0019 0.0011 0.0013 0.0013 0.0006 0.002 0.0011 0.0012
Vacuole Periphery 0.0015 0.0003 0.0005 0.0004 0.0008 0.0006 0.0007 0.0002 0.0005 0.0002 0.0012 0.0002 0.0003 0.0002 0.0001 0.0004 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 61.3002 56.5664 40.663 61.5572 42.303 51.3846 55.4069 55.4907 62.3392 67.5985
Translational Efficiency 1.7563 1.7621 2.2205 1.4121 2.481 2.3019 1.3767 2.0051 1.5861 1.47

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (69%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-6

Pbp4

Pbp4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pbp4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available