Standard name
Human Ortholog
Description Essential protein involved in vesicle-mediated ER to Golgi transport; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.05 0.12 0 0.06 0 0 0 0 0 0 0.13 0.08 0 0.15 0.22 0.18 0 0.11 0 0 0.12 0.12
Bud 0 0 0.09 0.11 0.09 0.09 0.11 0.09 0.12 0.13 0.11 0 0.06 0 0 0.09 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.09 0.13 0 0 0.15 0 0.08 0 0 0 0 0.07 0.08 0.22 0.12 0.13 0.17 0.15 0.12 0.28 0.14 0.13 0.12
Golgi 0.23 0.29 0.05 0 0.08 0 0 0 0 0 0 0.55 0.25 0.29 0.57 0.57 0.56 0.59 0.63 0.42 0.65 0.59 0.62
Mitochondria 0.86 0.84 1.0 0.86 0.76 0.82 0.68 0.8 0.81 0.83 0.83 0.75 0.7 0.52 0.09 0.07 0.11 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0.05 0.28 0.3 0.26 0.32 0.2 0.24 0.19 0.27 0.06 0.32 0.32 0.08 0.1 0.1 0.15 0.09 0.24 0.17 0.13 0.08
SpindlePole 0 0 0 0.05 0 0.08 0.14 0.14 0.12 0.12 0.1 0 0 0.05 0.12 0.09 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 15 2 8 12 24 8 20 7 12 8 14 39 35 14 28 39 34 9 6 0 1 33 45
Bud 5 0 6 35 36 46 94 45 72 63 76 11 30 17 8 16 13 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 1 4 9 8 12 10 22 0 0 1 1 1 0
Cell Periphery 0 0 1 0 3 1 2 3 3 2 0 0 0 0 1 0 4 0 0 0 0 0 0
Cytoplasm 4 1 0 8 6 6 9 9 1 6 4 6 11 16 10 9 12 0 0 0 0 0 0
Endoplasmic Reticulum 10 1 0 0 1 1 1 0 1 0 1 20 2 0 5 4 5 0 0 0 0 1 2
Endosome 19 5 0 9 57 17 72 20 26 7 13 22 39 112 23 23 32 34 7 23 10 36 46
Golgi 49 11 3 5 33 1 3 0 6 1 1 162 118 145 104 100 106 136 35 34 51 170 231
Mitochondria 181 32 64 282 297 403 596 403 484 388 599 223 326 263 16 13 20 9 0 4 1 6 7
Nucleus 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 3 0 3 3 2 2 0 0 2 0 1 0 1 0 0 0 0 0
Nucleolus 3 1 0 4 1 10 42 14 16 12 18 0 4 3 2 2 1 0 0 0 0 0 0
Peroxisomes 11 1 3 93 118 129 283 98 145 90 192 17 149 164 15 17 19 33 5 20 13 36 31
SpindlePole 4 0 0 15 17 40 127 68 75 54 74 2 14 24 22 16 18 1 0 0 0 0 2
Vac/Vac Membrane 1 1 0 1 1 4 1 5 3 2 1 4 0 6 12 5 7 1 1 0 0 0 2
Unique Cell Count 210 38 64 329 390 490 881 502 600 468 720 296 464 505 184 176 190 232 57 83 79 288 375
Labelled Cell Count 302 55 85 466 596 673 1259 684 859 645 1018 506 728 767 247 247 271 232 57 83 79 288 375


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 10.6 5.4 6.4 6.9 5.8 5.7 5.3 5.5 5.2 5.1 9.4 9.7 9.8 12.8 13.1 12.9 8.1 9.5 9.7
Std Deviation (1e-4) 1.4 1.8 0.8 1.1 1.0 0.8 1.0 1.4 1.0 0.9 1.0 1.2 1.6 1.8 2.8 3.1 2.5 1.3 1.6 1.5
Intensity Change (Log2) 0.26 0.37 0.11 0.09 -0.03 0.04 -0.06 -0.07 0.8 0.86 0.87 1.25 1.29 1.27 0.59 0.82 0.85


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0.1 -1.4 0 0.5 1.7 1.0
Bud 0.3 0 0 0.3 -0.1 0.6 0.9 0.3 0 0 0 0 -0.1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.3 0 0 0 0 0 0 0 0 4.2 3.0 3.0 3.5
Golgi 0 1.0 0 0 0 0 0 0 7.3 3.7 4.1 7.2 7.2 7.1
Mitochondria -3.2 -4.4 -3.7 -5.4 -3.9 -3.9 -3.6 -3.6 -4.4 -5.1 -7.3 -13.5 -13.6 -13.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 4.0 4.3 3.8 4.6 2.9 3.6 2.9 3.9 0 4.5 4.6 0 1.2 1.3
SpindlePole 0 0 0 3.3 3.1 3.0 2.9 2.7 0 0 0 2.9 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1008 5.4985 4.9224 4.3229 4.3628 5.1056 3.5296 5.2417 4.4723 4.4419 3.8018 4.4543 5.3968 6.9925 6.5289 6.5616 5.9455 6.4868
Actin 0.0555 0.0424 0.0239 0.1097 0.0575 0.0625 0.0429 0.0264 0.0367 0.004 0.0013 0.0684 0.0435 0.0171 0.0162 0.1062 0.0226 0.0961
Bud 0.0004 0.0002 0.0001 0.0003 0.0004 0.0022 0.0005 0.001 0.0025 0.0001 0 0.0001 0.0008 0.0005 0.0005 0.0001 0.0005 0.0002
Bud Neck 0.0041 0.0005 0.001 0.0015 0.0006 0.0011 0.0008 0.0004 0.0003 0.0003 0.0001 0.0004 0.0088 0.0004 0.0005 0.0002 0.0081 0.0006
Bud Periphery 0.0004 0.0001 0.0001 0.0006 0.0006 0.0011 0.0004 0.0007 0.0023 0 0 0.0001 0.0006 0.0002 0.0011 0 0.0007 0.0001
Bud Site 0.0024 0.0043 0.0003 0.0009 0.0009 0.0009 0.0117 0.005 0.0025 0.0002 0.0001 0.0002 0.0178 0.0053 0.0014 0.0001 0.003 0.0003
Cell Periphery 0.0001 0 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0.0002 0 0.0002 0 0.0004 0
Cytoplasm 0.0026 0.001 0.0021 0.0007 0.0008 0.0056 0.0044 0.0014 0.0014 0.0012 0.0006 0.0009 0.0171 0.0014 0.0013 0.0005 0.0011 0.0007
Cytoplasmic Foci 0.1737 0.2094 0.152 0.1168 0.1394 0.1253 0.1535 0.1583 0.1163 0.1664 0.1152 0.0879 0.1823 0.1758 0.1412 0.1392 0.12 0.0967
Eisosomes 0.0002 0 0.0001 0.0004 0.0003 0.0001 0.0002 0 0.0001 0 0 0.0001 0.0001 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0012 0.0001 0.0005 0.0006 0.0025 0.0002 0.0023 0.0006 0.0003 0.0002 0.0002 0.0013 0.0016 0.0002 0.0005 0.0001 0.0006 0.0002
Endosome 0.1333 0.1119 0.1023 0.1026 0.1065 0.0621 0.1218 0.1054 0.1048 0.1529 0.1578 0.0822 0.1632 0.1549 0.1449 0.104 0.1521 0.065
Golgi 0.4167 0.5042 0.5635 0.5281 0.3493 0.4912 0.4759 0.6081 0.5904 0.6145 0.7018 0.6542 0.3088 0.5032 0.5746 0.6122 0.5509 0.5802
Lipid Particles 0.0433 0.0126 0.0211 0.0475 0.0957 0.0464 0.0348 0.0093 0.0299 0.0094 0.013 0.0172 0.0597 0.0195 0.0246 0.0074 0.0106 0.0385
Mitochondria 0.0106 0.0032 0.0151 0.0135 0.0095 0.013 0.0158 0.0041 0.0115 0.0032 0.0034 0.0056 0.0474 0.0053 0.022 0.0029 0.0257 0.0069
None 0.0054 0 0.0001 0.0003 0.0007 0.0013 0.0023 0 0.0002 0.0001 0 0.0001 0.0044 0 0.0006 0 0.0001 0
Nuclear Periphery 0.0067 0 0.0002 0.0009 0.0093 0.0001 0.0037 0.0002 0.0001 0 0 0.0009 0.001 0.0001 0.0007 0 0.0003 0
Nucleolus 0.0006 0 0 0.0002 0.0003 0.0004 0.0008 0 0.0002 0 0 0 0.0003 0.0001 0.001 0 0.0012 0
Nucleus 0.0018 0 0 0.0003 0.0006 0.0005 0.0013 0 0.0001 0 0 0.0001 0.0006 0 0.0007 0 0.0006 0
Peroxisomes 0.1267 0.1093 0.1162 0.0724 0.217 0.1839 0.121 0.0775 0.0978 0.0463 0.0055 0.0794 0.135 0.1146 0.0615 0.0267 0.0816 0.1137
Punctate Nuclear 0.0126 0.0002 0.0005 0.0011 0.0056 0.0009 0.0031 0.0001 0.0003 0.0002 0.0001 0.0001 0.0038 0.0002 0.0004 0.0001 0.0004 0.0002
Vacuole 0.0007 0.0002 0.0003 0.0005 0.0008 0.0005 0.0016 0.0007 0.0016 0.0005 0.0003 0.0002 0.0016 0.0006 0.0041 0.0001 0.0162 0.0002
Vacuole Periphery 0.0008 0.0003 0.0004 0.0008 0.0016 0.0005 0.0011 0.0007 0.0006 0.0005 0.0006 0.0005 0.0013 0.0005 0.0021 0.0003 0.0035 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.3802 37.6339 31.6041 38.754 42.2021 38.176 34.3393 33.387 37.9944 42.4165
Translational Efficiency 0.9791 0.8478 0.8697 0.8448 0.7593 0.7848 0.6553 0.6371 0.6334 0.7153

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Essential protein involved in vesicle-mediated ER to Golgi transport; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
Localization
Cell Percentages Golgi (34%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Uso1

Uso1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Uso1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available