Standard name | |
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Human Ortholog | |
Description | Essential protein involved in vesicle-mediated ER to Golgi transport; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 15 | 2 | 8 | 12 | 24 | 8 | – | 20 | 7 | 12 | 8 | 14 | 39 | 35 | 14 | 28 | 39 | 34 | 9 | 6 | 0 | 1 | 33 | 45 |
Bud | 5 | 0 | 6 | 35 | 36 | 46 | – | 94 | 45 | 72 | 63 | 76 | 11 | 30 | 17 | 8 | 16 | 13 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 4 | – | 9 | 8 | 12 | 10 | 22 | 0 | 0 | 1 | 1 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 3 | 1 | – | 2 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 1 | 0 | 8 | 6 | 6 | – | 9 | 9 | 1 | 6 | 4 | 6 | 11 | 16 | 10 | 9 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 10 | 1 | 0 | 0 | 1 | 1 | – | 1 | 0 | 1 | 0 | 1 | 20 | 2 | 0 | 5 | 4 | 5 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 19 | 5 | 0 | 9 | 57 | 17 | – | 72 | 20 | 26 | 7 | 13 | 22 | 39 | 112 | 23 | 23 | 32 | 34 | 7 | 23 | 10 | 36 | 46 |
Golgi | 49 | 11 | 3 | 5 | 33 | 1 | – | 3 | 0 | 6 | 1 | 1 | 162 | 118 | 145 | 104 | 100 | 106 | 136 | 35 | 34 | 51 | 170 | 231 |
Mitochondria | 181 | 32 | 64 | 282 | 297 | 403 | – | 596 | 403 | 484 | 388 | 599 | 223 | 326 | 263 | 16 | 13 | 20 | 9 | 0 | 4 | 1 | 6 | 7 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 3 | – | 0 | 3 | 3 | 2 | 2 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | 1 | 0 | 4 | 1 | 10 | – | 42 | 14 | 16 | 12 | 18 | 0 | 4 | 3 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 11 | 1 | 3 | 93 | 118 | 129 | – | 283 | 98 | 145 | 90 | 192 | 17 | 149 | 164 | 15 | 17 | 19 | 33 | 5 | 20 | 13 | 36 | 31 |
SpindlePole | 4 | 0 | 0 | 15 | 17 | 40 | – | 127 | 68 | 75 | 54 | 74 | 2 | 14 | 24 | 22 | 16 | 18 | 1 | 0 | 0 | 0 | 0 | 2 |
Vac/Vac Membrane | 1 | 1 | 0 | 1 | 1 | 4 | – | 1 | 5 | 3 | 2 | 1 | 4 | 0 | 6 | 12 | 5 | 7 | 1 | 1 | 0 | 0 | 0 | 2 |
Unique Cell Count | 210 | 38 | 64 | 329 | 390 | 490 | 881 | 502 | 600 | 468 | 720 | 296 | 464 | 505 | 184 | 176 | 190 | 232 | 57 | 83 | 79 | 288 | 375 | |
Labelled Cell Count | 302 | 55 | 85 | 466 | 596 | 673 | 1259 | 684 | 859 | 645 | 1018 | 506 | 728 | 767 | 247 | 247 | 271 | 232 | 57 | 83 | 79 | 288 | 375 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.3 | 10.6 | 5.4 | 6.4 | 6.9 | 5.8 | – | 5.7 | 5.3 | 5.5 | 5.2 | 5.1 | 9.4 | 9.7 | 9.8 | 12.8 | 13.1 | 12.9 | 8.1 | 9.5 | 9.7 |
Std Deviation (1e-4) | 1.4 | 1.8 | 0.8 | 1.1 | 1.0 | 0.8 | – | 1.0 | 1.4 | 1.0 | 0.9 | 1.0 | 1.2 | 1.6 | 1.8 | 2.8 | 3.1 | 2.5 | 1.3 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.26 | 0.37 | 0.11 | – | 0.09 | -0.03 | 0.04 | -0.06 | -0.07 | 0.8 | 0.86 | 0.87 | 1.25 | 1.29 | 1.27 | 0.59 | 0.82 | 0.85 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0.1 | -1.4 | 0 | 0.5 | 1.7 | 1.0 |
Bud | 0.3 | 0 | 0 | – | 0.3 | -0.1 | 0.6 | 0.9 | 0.3 | 0 | 0 | 0 | 0 | -0.1 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 3.3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 3.0 | 3.0 | 3.5 |
Golgi | 0 | 1.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 7.3 | 3.7 | 4.1 | 7.2 | 7.2 | 7.1 |
Mitochondria | -3.2 | -4.4 | -3.7 | – | -5.4 | -3.9 | -3.9 | -3.6 | -3.6 | -4.4 | -5.1 | -7.3 | -13.5 | -13.6 | -13.2 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 4.0 | 4.3 | 3.8 | – | 4.6 | 2.9 | 3.6 | 2.9 | 3.9 | 0 | 4.5 | 4.6 | 0 | 1.2 | 1.3 |
SpindlePole | 0 | 0 | 0 | – | 3.3 | 3.1 | 3.0 | 2.9 | 2.7 | 0 | 0 | 0 | 2.9 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.1008 | 5.4985 | 4.9224 | 4.3229 | 4.3628 | 5.1056 | 3.5296 | 5.2417 | 4.4723 | 4.4419 | 3.8018 | 4.4543 | 5.3968 | 6.9925 | 6.5289 | 6.5616 | 5.9455 | 6.4868 |
Actin | 0.0555 | 0.0424 | 0.0239 | 0.1097 | 0.0575 | 0.0625 | 0.0429 | 0.0264 | 0.0367 | 0.004 | 0.0013 | 0.0684 | 0.0435 | 0.0171 | 0.0162 | 0.1062 | 0.0226 | 0.0961 |
Bud | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0022 | 0.0005 | 0.001 | 0.0025 | 0.0001 | 0 | 0.0001 | 0.0008 | 0.0005 | 0.0005 | 0.0001 | 0.0005 | 0.0002 |
Bud Neck | 0.0041 | 0.0005 | 0.001 | 0.0015 | 0.0006 | 0.0011 | 0.0008 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0.0088 | 0.0004 | 0.0005 | 0.0002 | 0.0081 | 0.0006 |
Bud Periphery | 0.0004 | 0.0001 | 0.0001 | 0.0006 | 0.0006 | 0.0011 | 0.0004 | 0.0007 | 0.0023 | 0 | 0 | 0.0001 | 0.0006 | 0.0002 | 0.0011 | 0 | 0.0007 | 0.0001 |
Bud Site | 0.0024 | 0.0043 | 0.0003 | 0.0009 | 0.0009 | 0.0009 | 0.0117 | 0.005 | 0.0025 | 0.0002 | 0.0001 | 0.0002 | 0.0178 | 0.0053 | 0.0014 | 0.0001 | 0.003 | 0.0003 |
Cell Periphery | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0004 | 0 |
Cytoplasm | 0.0026 | 0.001 | 0.0021 | 0.0007 | 0.0008 | 0.0056 | 0.0044 | 0.0014 | 0.0014 | 0.0012 | 0.0006 | 0.0009 | 0.0171 | 0.0014 | 0.0013 | 0.0005 | 0.0011 | 0.0007 |
Cytoplasmic Foci | 0.1737 | 0.2094 | 0.152 | 0.1168 | 0.1394 | 0.1253 | 0.1535 | 0.1583 | 0.1163 | 0.1664 | 0.1152 | 0.0879 | 0.1823 | 0.1758 | 0.1412 | 0.1392 | 0.12 | 0.0967 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0012 | 0.0001 | 0.0005 | 0.0006 | 0.0025 | 0.0002 | 0.0023 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0013 | 0.0016 | 0.0002 | 0.0005 | 0.0001 | 0.0006 | 0.0002 |
Endosome | 0.1333 | 0.1119 | 0.1023 | 0.1026 | 0.1065 | 0.0621 | 0.1218 | 0.1054 | 0.1048 | 0.1529 | 0.1578 | 0.0822 | 0.1632 | 0.1549 | 0.1449 | 0.104 | 0.1521 | 0.065 |
Golgi | 0.4167 | 0.5042 | 0.5635 | 0.5281 | 0.3493 | 0.4912 | 0.4759 | 0.6081 | 0.5904 | 0.6145 | 0.7018 | 0.6542 | 0.3088 | 0.5032 | 0.5746 | 0.6122 | 0.5509 | 0.5802 |
Lipid Particles | 0.0433 | 0.0126 | 0.0211 | 0.0475 | 0.0957 | 0.0464 | 0.0348 | 0.0093 | 0.0299 | 0.0094 | 0.013 | 0.0172 | 0.0597 | 0.0195 | 0.0246 | 0.0074 | 0.0106 | 0.0385 |
Mitochondria | 0.0106 | 0.0032 | 0.0151 | 0.0135 | 0.0095 | 0.013 | 0.0158 | 0.0041 | 0.0115 | 0.0032 | 0.0034 | 0.0056 | 0.0474 | 0.0053 | 0.022 | 0.0029 | 0.0257 | 0.0069 |
None | 0.0054 | 0 | 0.0001 | 0.0003 | 0.0007 | 0.0013 | 0.0023 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0044 | 0 | 0.0006 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.0067 | 0 | 0.0002 | 0.0009 | 0.0093 | 0.0001 | 0.0037 | 0.0002 | 0.0001 | 0 | 0 | 0.0009 | 0.001 | 0.0001 | 0.0007 | 0 | 0.0003 | 0 |
Nucleolus | 0.0006 | 0 | 0 | 0.0002 | 0.0003 | 0.0004 | 0.0008 | 0 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.001 | 0 | 0.0012 | 0 |
Nucleus | 0.0018 | 0 | 0 | 0.0003 | 0.0006 | 0.0005 | 0.0013 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0006 | 0 | 0.0007 | 0 | 0.0006 | 0 |
Peroxisomes | 0.1267 | 0.1093 | 0.1162 | 0.0724 | 0.217 | 0.1839 | 0.121 | 0.0775 | 0.0978 | 0.0463 | 0.0055 | 0.0794 | 0.135 | 0.1146 | 0.0615 | 0.0267 | 0.0816 | 0.1137 |
Punctate Nuclear | 0.0126 | 0.0002 | 0.0005 | 0.0011 | 0.0056 | 0.0009 | 0.0031 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0038 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0002 |
Vacuole | 0.0007 | 0.0002 | 0.0003 | 0.0005 | 0.0008 | 0.0005 | 0.0016 | 0.0007 | 0.0016 | 0.0005 | 0.0003 | 0.0002 | 0.0016 | 0.0006 | 0.0041 | 0.0001 | 0.0162 | 0.0002 |
Vacuole Periphery | 0.0008 | 0.0003 | 0.0004 | 0.0008 | 0.0016 | 0.0005 | 0.0011 | 0.0007 | 0.0006 | 0.0005 | 0.0006 | 0.0005 | 0.0013 | 0.0005 | 0.0021 | 0.0003 | 0.0035 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 41.3802 | 37.6339 | 31.6041 | 38.754 | 42.2021 | 38.176 | 34.3393 | 33.387 | 37.9944 | 42.4165 |
Translational Efficiency | 0.9791 | 0.8478 | 0.8697 | 0.8448 | 0.7593 | 0.7848 | 0.6553 | 0.6371 | 0.6334 | 0.7153 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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