Standard name
Human Ortholog
Description Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0 – – – 0 0 0.09 0 0 0.08
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0 0 – – – 0 0.1 0.05 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 – – – 0 0 0 0 0 0
Cytoplasm 0.84 0.87 0.71 0.73 0.72 0.71 0.63 0.71 0.64 0.65 0.57 0.6 0.91 0.93 0.94 – – – 0.73 0.71 0.45 0.73 0.79 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0.05 0 0 0
Endosome 0 0 0 0.07 0.07 0.07 0.06 0 0 0.05 0 0.05 0 0 0 – – – 0.09 0 0.07 0.05 0 0
Golgi 0 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0.05 0.14 0.28 0.11 0.33 0.32 0.35 0.24 0 0 0 – – – 0 0 0.07 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0.05 0 0 0.05 0 0 0 0 – – – 0 0 0.07 0 0 0
Vac/Vac Membrane 0 0 0.09 0.15 0.09 0.09 0.07 0.09 0.07 0.06 0 0.06 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 5 0 6 1 2 9 0 2 7 5 0 3 2 – – – 3 4 12 5 6 12
Bud 3 3 4 0 4 4 3 7 5 5 8 16 0 0 0 – – – 3 18 6 4 7 5
Bud Neck 0 0 0 0 0 0 1 1 1 0 1 0 0 0 0 – – – 2 7 3 1 4 3
Bud Site 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 1 2 1 6 4 5 9 4 7 4 10 1 7 3 – – – 0 0 0 0 0 0
Cytoplasm 91 141 70 85 155 98 128 197 89 132 74 113 50 127 125 – – – 82 131 57 85 132 117
Endoplasmic Reticulum 1 4 0 0 3 4 3 2 2 0 1 0 2 3 1 – – – 0 1 7 4 2 1
Endosome 2 4 2 8 15 10 13 5 5 10 2 9 2 1 2 – – – 10 4 8 6 4 4
Golgi 1 5 7 2 10 0 1 3 0 0 0 0 1 4 3 – – – 1 2 4 1 0 0
Mitochondria 13 5 2 5 11 19 56 30 45 64 46 45 1 0 0 – – – 1 0 8 1 1 2
Nucleus 0 0 0 1 2 1 1 6 0 1 3 1 1 0 0 – – – 0 0 0 0 0 1
Nuclear Periphery 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 1 1 2 0 0 4 7 5 1 2 3 0 0 0 – – – 0 1 0 0 0 0
Peroxisomes 0 0 1 1 0 2 3 0 0 0 0 2 0 0 0 – – – 1 1 1 0 2 0
SpindlePole 0 0 5 5 7 2 3 14 2 4 6 8 0 0 1 – – – 2 6 9 2 2 1
Vac/Vac Membrane 1 0 9 18 20 13 15 24 9 13 3 12 1 0 0 – – – 0 3 0 0 1 0
Unique Cell Count 108 162 99 117 215 138 202 277 138 202 130 187 55 136 133 113 185 130 118 169 156
Labelled Cell Count 114 168 108 129 239 158 239 315 168 240 157 224 59 145 137 113 185 130 118 169 156


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 11.0 10.1 10.7 9.4 8.3 7.6 8.8 6.8 6.8 7.0 6.6 8.9 8.5 8.6 – – – 8.2 8.8 10.2
Std Deviation (1e-4) 1.4 1.6 2.9 4.9 2.7 2.9 2.8 2.8 2.1 2.0 1.7 1.5 1.8 1.5 1.8 – – – 1.5 1.5 2.0
Intensity Change (Log2) – – – 0.08 -0.1 -0.29 -0.41 -0.19 -0.56 -0.56 -0.53 -0.62 -0.19 -0.24 -0.23 – – – -0.29 -0.19 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0.1 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0.7 1.4 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0.3 0.3 0.1 -1.3 0.1 -1.0 -0.9 -2.1 -1.7 2.9 4.7 4.8 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 1.8 1.8 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 -0.9 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 3.1 5.3 2.7 5.8 5.8 6.1 4.8 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 1.4 0.1 0.1 -0.5 -0.1 -0.7 -0.8 -2.3 -0.8 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9455 6.3278 5.9377 5.3266 5.5026 5.9502 3.921 6.1905 5.378 4.9736 4.9072 5.3279 9.9809 11.324 10.7457 10.3927 9.9388 10.2932
Actin 0.0433 0.0563 0.0343 0.0189 0.0828 0.0347 0.0304 0.0301 0.0245 0.0325 0.0557 0.0196 0.0839 0.0667 0.0762 0.0965 0.0693 0.1103
Bud 0.0047 0.0101 0.0043 0.0018 0.0012 0.0018 0.0094 0.0088 0.0116 0.0084 0.0148 0.0029 0.0122 0.0183 0.0245 0.0253 0.0245 0.0058
Bud Neck 0.0055 0.0022 0.0229 0.0334 0.0119 0.0232 0.0025 0.0034 0.0334 0.0685 0.0128 0.0194 0.0042 0.0084 0.0212 0.0772 0.0037 0.0223
Bud Periphery 0.0033 0.005 0.0021 0.0009 0.001 0.0013 0.0085 0.0057 0.009 0.0052 0.0133 0.0024 0.0122 0.0074 0.0085 0.018 0.0145 0.0037
Bud Site 0.0319 0.0763 0.0225 0.0074 0.0014 0.0015 0.0356 0.1222 0.0381 0.0107 0.0089 0.0026 0.075 0.1729 0.0594 0.0095 0.006 0.0032
Cell Periphery 0.0008 0.0008 0.0003 0.0002 0.0001 0.0002 0.0006 0.0002 0.0005 0.0004 0.0004 0.0001 0.0025 0.0013 0.0013 0.0012 0.0008 0.0006
Cytoplasm 0.5236 0.3685 0.5214 0.4247 0.3643 0.4082 0.3822 0.3293 0.3898 0.2719 0.2014 0.3405 0.4134 0.3469 0.3672 0.3215 0.2856 0.3837
Cytoplasmic Foci 0.2145 0.2947 0.2028 0.2657 0.2145 0.3169 0.2791 0.2863 0.2682 0.3037 0.416 0.3318 0.133 0.1997 0.2372 0.2245 0.2445 0.2715
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0 0 0.0004 0.0001 0.0003 0.0005 0.0002 0.0001
Endoplasmic Reticulum 0.0119 0.0018 0.0099 0.0041 0.0051 0.0046 0.0054 0.0011 0.0031 0.0018 0.0011 0.0014 0.0221 0.0028 0.0023 0.0038 0.0043 0.0085
Endosome 0.0711 0.0676 0.0832 0.147 0.1766 0.0871 0.0976 0.0881 0.079 0.1523 0.1013 0.118 0.0596 0.024 0.0404 0.0378 0.0752 0.0704
Golgi 0.0348 0.0273 0.0205 0.0168 0.0451 0.0263 0.0305 0.0335 0.0249 0.0284 0.0276 0.0347 0.0172 0.0191 0.017 0.0193 0.0259 0.0204
Lipid Particles 0.0044 0.0011 0.005 0.0057 0.0077 0.0025 0.0037 0.0016 0.0025 0.0033 0.0023 0.0034 0.0096 0.0024 0.0045 0.0029 0.0069 0.0029
Mitochondria 0.0031 0.0017 0.0025 0.0016 0.0091 0.003 0.0042 0.0019 0.0027 0.0046 0.0029 0.0053 0.0113 0.0075 0.0157 0.0164 0.0085 0.0379
None 0.0083 0.0043 0.0067 0.0121 0.0023 0.0033 0.0132 0.0036 0.0044 0.0051 0.0026 0.004 0.0702 0.023 0.0367 0.0376 0.0437 0.0199
Nuclear Periphery 0.0043 0.0003 0.0016 0.0011 0.0068 0.0049 0.001 0.0003 0.0007 0.0007 0.0004 0.0003 0.0094 0.0004 0.0032 0.0015 0.0016 0.0008
Nucleolus 0.0003 0.0006 0.0005 0.0005 0.0007 0.0003 0.0003 0.0004 0.0003 0.0004 0.0002 0.0001 0.001 0.0028 0.0036 0.0008 0.0004 0.002
Nucleus 0.0011 0.0013 0.0009 0.0008 0.0032 0.0028 0.0014 0.0011 0.0012 0.0014 0.0008 0.0008 0.0063 0.0023 0.0089 0.0021 0.0014 0.0014
Peroxisomes 0.0255 0.0663 0.049 0.0413 0.0505 0.0682 0.0836 0.0733 0.0977 0.0882 0.1275 0.1058 0.0376 0.0872 0.0629 0.0951 0.1538 0.0286
Punctate Nuclear 0.0027 0.0012 0.0033 0.0025 0.0045 0.0036 0.0018 0.004 0.0016 0.0036 0.0053 0.0012 0.0113 0.0033 0.0041 0.003 0.0042 0.0028
Vacuole 0.004 0.0118 0.0052 0.0095 0.0073 0.0045 0.008 0.0043 0.0058 0.0069 0.0038 0.0046 0.0061 0.003 0.0039 0.0044 0.0191 0.0022
Vacuole Periphery 0.0009 0.0008 0.0011 0.0039 0.0035 0.001 0.0011 0.0007 0.001 0.0021 0.0009 0.001 0.0013 0.0006 0.0011 0.0011 0.0061 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0123 15.3372 28.4522 24.3252 31.2816 27.8646 21.0805 20.933 31.4147 29.1887
Translational Efficiency 6.0076 4.177 1.6087 2.6109 2.222 3.0747 3.1969 2.5487 2.0282 2.3517

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1314 265 1022 1489 1705 1548 2603 1076 3019 1813 3625 2565

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 822.55 1020.32 1220.33 1436.46 896.86 1064.58 1207.34 1505.67 864.52 1058.11 1211.00 1465.49
Standard Deviation 136.15 194.80 164.77 238.35 133.69 163.12 159.67 272.66 139.71 168.85 161.23 255.60
Intensity Change Log 2 — 0.310846 0.569096 0.804343 — 0.247330 0.428877 0.747451 — 0.278065 0.497659 0.774947

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005657 0.017336 0.023349 0.086924 0.003728 0.019797 0.015530 0.096371 0.004568 0.019438 0.017734 0.090887
Bud Neck 0.020454 0.014047 0.005413 0.004608 0.022191 0.010331 0.006209 0.005795 0.021435 0.010874 0.005985 0.005106
Bud Site 0.155622* 0.200092* 0.156726* 0.234347* 0.161005* 0.172699* 0.097447 0.200694* 0.158662* 0.176703* 0.114159* 0.220230*
Cell Periphery 0.000466 0.000386 0.000175 0.002144 0.000657 0.000222 0.000130 0.001096 0.000574 0.000246 0.000143 0.001705
Cytoplasm 0.389843* 0.287402* 0.350391* 0.451170* 0.460781* 0.422900* 0.499941* 0.357692* 0.429906* 0.403095* 0.457779* 0.411957*
Cytoplasmic Foci 0.252491* 0.273641* 0.233575* 0.111369 0.216277* 0.227144* 0.165683 0.150218 0.232039* 0.233941* 0.184824* 0.127666
Eisosomes 0.000221 0.000073 0.000039 0.000072 0.000071 0.000085 0.000030 0.000063 0.000136 0.000083 0.000033 0.000068
Endoplasmic Reticulum 0.005469 0.002620 0.000699 0.000484 0.005333 0.001729 0.000485 0.000335 0.005392 0.001860 0.000545 0.000422
Endosome 0.036293 0.043835 0.027288 0.009293 0.024295 0.022214 0.035253 0.019869 0.029517 0.025374 0.033007 0.013729
Golgi 0.028809 0.039507 0.008509 0.016322 0.023027 0.032655 0.010290 0.026309 0.025544 0.033657 0.009788 0.020511
Lipid Particles 0.004103 0.002359 0.002793 0.009837 0.001803 0.001646 0.002479 0.008663 0.002804 0.001750 0.002567 0.009345
Mitochondria 0.009607 0.002630 0.000101 0.000733 0.002259 0.001701 0.000557 0.001678 0.005457 0.001836 0.000428 0.001130
Mitotic Spindle 0.006320 0.004965 0.055075 0.011249 0.006646 0.015663 0.020468 0.029866 0.006504 0.014100 0.030225 0.019059
None 0.001367 0.000301 0.000054 0.000090 0.000548 0.000148 0.000184 0.000074 0.000904 0.000171 0.000147 0.000084
Nuclear Periphery 0.000070 0.000104 0.000054 0.000025 0.000029 0.000037 0.000037 0.000100 0.000047 0.000047 0.000042 0.000057
Nuclear Periphery Foci 0.000701 0.000525 0.000955 0.000595 0.000313 0.000523 0.000359 0.000605 0.000482 0.000523 0.000527 0.000599
Nucleolus 0.000297 0.000149 0.000043 0.000099 0.000200 0.000094 0.000221 0.000070 0.000242 0.000102 0.000171 0.000087
Nucleus 0.005709 0.001161 0.001402 0.001098 0.003178 0.001061 0.001942 0.000827 0.004280 0.001076 0.001790 0.000984
Peroxisomes 0.026219 0.075347 0.065717 0.042178 0.022763 0.049374 0.057065 0.072844 0.024267 0.053170 0.059504 0.055042
Vacuole 0.041130 0.030400 0.067049 0.015269 0.037654 0.017724 0.084553 0.023659 0.039167 0.019576 0.079618 0.018789
Vacuole Periphery 0.009151 0.003119 0.000592 0.002092 0.007243 0.002252 0.001138 0.003171 0.008074 0.002379 0.000984 0.002545

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.13 -5.35 -15.76 -9.09 -11.36 -7.43 -8.02 -13.82 -10.47 -11.75 -7.34 -9.19 -21.13 -15.32 -17.33
Bud Neck 2.22 9.64 10.00 3.70 1.50 7.54 11.01 11.10 5.53 0.92 8.42 14.39 15.18 7.73 2.21
Bud Site -2.70 1.01 -8.47 -1.93 -9.75 -1.43 10.55 -3.58 -2.21 -13.49 -2.60 9.80 -9.05 -5.40 -19.92
Cell Periphery 1.24 7.20 -4.23 -4.43 -5.04 3.61 4.43 -1.01 -2.52 -2.84 4.63 6.21 -3.94 -5.41 -5.84
Cytoplasm 4.97 2.12 -5.82 -8.35 -7.28 3.12 -3.94 5.91 3.05 9.53 2.64 -4.00 0.22 -2.29 3.89
Cytoplasmic Foci -1.28 3.53 19.85 10.64 13.69 -1.39 7.78 9.05 10.28 2.52 -0.24 9.83 20.49 17.45 11.20
Eisosomes 1.95 2.46 2.03 0.25 -4.69 -1.06 4.04 1.35 3.44 -5.98 1.60 3.17 2.19 2.59 -7.95
Endoplasmic Reticulum 1.26 4.92 5.32 1.03 0.48 3.68 5.61 5.83 3.03 1.27 4.39 7.57 7.84 2.87 0.77
Endosome -1.29 4.18 13.41 6.44 8.81 1.12 -4.71 3.13 2.03 7.41 2.37 -1.46 12.31 7.80 12.62
Golgi -2.05 11.77 6.04 4.80 -4.77 -4.30 9.60 0.24 3.36 -5.10 -4.01 14.68 4.37 7.09 -6.49
Lipid Particles 1.88 2.07 -4.68 -5.27 -5.66 0.50 -2.73 -8.53 -8.42 -7.49 3.19 0.30 -8.54 -9.61 -8.67
Mitochondria 3.32 7.49 6.94 1.13 -2.80 0.97 5.00 1.40 0.42 -1.71 4.82 8.54 7.03 1.60 -2.29
Mitotic Spindle 0.93 -10.45 -3.55 -4.16 8.97 -4.15 -8.00 -8.28 -5.48 -3.25 -4.36 -13.16 -8.65 -3.83 4.44
None 3.12 4.57 4.41 1.04 -2.92 4.76 2.87 5.42 1.87 0.78 5.36 5.03 6.01 1.96 0.61
Nuclear Periphery -0.39 -0.56 3.65 0.83 7.43 -0.46 -3.19 -1.99 -1.63 -1.55 -0.02 -1.41 -0.97 -0.64 -0.52
Nuclear Periphery Foci 0.73 -2.33 0.24 -0.68 3.80 -1.03 -1.21 -3.64 -1.03 -3.06 -0.28 -1.51 -1.96 -0.88 -0.86
Nucleolus 1.66 5.73 1.22 0.03 -0.79 1.81 -0.12 0.55 -0.42 0.50 2.97 0.80 1.17 -0.44 0.25
Nucleus 6.66 6.15 6.81 -0.46 2.13 9.34 4.63 4.02 -1.26 1.35 10.20 7.33 8.38 -1.81 2.59
Peroxisomes -5.13 -7.27 -3.83 3.74 4.24 -7.85 -10.43 -9.23 -2.83 -1.99 -9.08 -12.41 -9.51 1.01 1.88
Vacuole 1.53 -7.83 8.90 3.01 12.96 7.63 -11.64 2.27 -4.82 13.29 9.05 -14.00 8.08 -1.59 19.92
Vacuole Periphery 4.72 12.39 7.94 0.93 -2.36 7.60 9.94 4.52 -0.55 -2.00 10.84 15.40 8.77 0.19 -2.74
Standard name
Human Ortholog
Description Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning
Localization
Cell Percentages cytoplasm (81%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Pex19

Pex19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available