Standard name
Human Ortholog
Description Mitochondrial NADP-specific isocitrate dehydrogenase; catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.05 0 0.06 0.07 0.08 0 0.08 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.09 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.4 0.28 0.46 0.31 0.34 0.09 0.14 0.07 0.17 0.2 0.25
Mitochondria 0.91 0.97 0.92 0.98 0.91 0.95 0.94 0.9 0.95 0.96 0.95 0.95 0.87 0.72 0.8 0.11 0.13 0.05 0.72 0.71 0.73 0.68 0.59 0.57
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.11 0.08 0.07 0.22 0.24 0.28 0.23 0.19 0.21 0.18 0.18 0.07 0.07 0 0.16 0.15 0.18 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.05 0 0.08 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0.05 0 0 0.05 0 0 0 0.09 0.1 0.08 0.12 0.22 0.21 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 7 0 0 1 1 2 2 2 1 1 1 4 13 14 3 1 8 7 28 7 23 16
Bud 1 0 4 4 0 1 4 3 2 9 3 7 1 1 2 2 0 0 0 0 0 1 4 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 2 1 2 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 3 1 0 1 0 2 1 0 1 0 3 8 2 4 2 0 0 0 0 0 0 0
Cytoplasm 1 0 4 1 8 0 3 10 6 4 1 3 4 2 6 14 5 7 0 0 0 1 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 1 0 0 1 1 2 0 0 3 3 0 0 0 0 0 1 0 0
Endosome 0 0 1 0 0 0 1 1 0 0 0 0 0 0 2 2 1 0 1 2 2 4 3 3
Golgi 2 2 5 1 4 1 3 4 4 0 4 1 32 151 92 65 17 13 13 14 23 42 55 65
Mitochondria 125 78 295 209 281 448 526 565 420 552 410 446 198 274 259 16 7 2 107 72 251 174 166 149
Nucleus 0 1 1 0 0 2 1 1 0 1 1 0 1 0 0 1 1 0 0 0 0 1 0 0
Nuclear Periphery 3 0 5 1 0 0 7 2 4 7 2 4 3 2 0 0 0 0 1 0 0 1 1 1
Nucleolus 27 9 26 15 67 115 154 147 86 122 76 83 17 26 7 22 8 7 3 2 3 3 5 4
Peroxisomes 2 0 13 1 0 0 0 2 1 0 1 1 5 7 7 2 4 1 7 1 28 4 5 6
SpindlePole 0 0 0 0 1 0 0 1 0 0 0 0 0 2 0 1 2 2 1 0 0 2 0 1
Vac/Vac Membrane 5 3 3 5 19 22 14 25 22 11 14 8 20 38 26 17 12 8 0 0 0 10 14 8
Unique Cell Count 137 80 322 214 308 471 557 629 443 577 432 470 228 381 323 141 55 38 149 102 343 258 282 265
Labelled Cell Count 166 93 367 238 380 591 716 767 549 711 516 559 285 515 419 163 62 41 149 102 343 258 282 265


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 28.1 40.5 33.8 42.1 49.7 46.1 42.2 39.1 37.7 34.7 34.8 32.8 35.8 35.4 33.6 47.8 44.2 42.3 38.4 39.1 40.7
Std Deviation (1e-4) 9.4 13.7 10.1 11.7 16.6 15.0 15.2 14.4 14.0 13.5 13.2 13.3 10.1 10.7 9.5 20.8 20.5 21.5 13.0 14.8 15.2
Intensity Change (Log2) – – – 0.32 0.55 0.45 0.32 0.21 0.16 0.04 0.04 -0.04 0.08 0.07 -0.01 0.5 0.39 0.32 0.18 0.21 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 -1.2 1.4 3.7 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1.2 0 0 0 0
Cytoplasm 0 1.2 0 0 0 0 0 0 0 0 0 0 4.5 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 5.8 12.1 9.6 12.3 0 0
Mitochondria 2.9 -0.2 2.0 1.6 -0.9 1.8 2.5 1.8 1.8 -1.8 -6.6 -4.2 -16.9 -13.5 -13.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.5 4.8 5.9 6.9 5.8 4.4 5.1 3.8 3.8 -0.3 -0.6 -3.4 2.4 0 0
Peroxisomes -2.5 -3.6 -4.4 0 -4.4 -3.9 0 -3.8 -4.0 -1.2 -1.7 -1.4 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.6 3.0 1.6 2.6 3.1 1.1 2.1 0 4.5 5.1 4.4 5.4 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 89.3782 108.8076 99.2152 91.683 94.1646 91.5667 96.3838 114.4875 97.2909 72.3436 95.8092 112.8615 107.9917 133.5066 124.7308 112.9176 107.1498 111.8569
Actin 0.0131 0.0006 0.0065 0.0001 0.0057 0.0003 0.0143 0.0045 0.0113 0.019 0.0002 0.0001 0.0126 0.0002 0.0006 0 0.0018 0.0001
Bud 0.0004 0 0.0001 0 0.0001 0 0.0001 0 0 0 0 0 0.0004 0 0 0 0 0
Bud Neck 0.0014 0 0.0004 0 0 0 0 0.0002 0.0001 0.0001 0 0 0.0002 0 0 0 0 0
Bud Periphery 0.0005 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0001 0 0 0.0004 0 0 0 0.0001 0
Bud Site 0.006 0 0.0076 0 0.0002 0 0.0002 0.0002 0.0001 0.0002 0 0 0.0006 0 0 0 0.0001 0
Cell Periphery 0.0003 0.0002 0.0015 0 0.0005 0.0001 0.0001 0.0007 0.0006 0.0001 0 0 0.0002 0.0001 0.0002 0.0001 0.0003 0
Cytoplasm 0.0054 0 0 0 0.0001 0.0006 0.0004 0 0 0 0 0 0.0004 0 0.0001 0 0.0001 0
Cytoplasmic Foci 0.011 0 0.0001 0 0.0041 0.0045 0.0072 0.0004 0.0012 0.0004 0 0 0.0117 0 0.0031 0 0.0028 0
Eisosomes 0.0022 0.0018 0.0036 0.0003 0.0007 0.0005 0.0017 0.009 0.0032 0.004 0.0033 0.0008 0.0041 0.0005 0.0046 0.0007 0.0033 0.0003
Endoplasmic Reticulum 0.0014 0 0 0 0 0 0 0 0 0 0 0 0.0004 0 0 0 0 0
Endosome 0.0053 0 0 0 0.0003 0 0.0002 0.0001 0.0001 0.0001 0 0 0.0036 0 0 0 0 0
Golgi 0.0025 0.0004 0.0002 0 0.0011 0.0001 0.0009 0.0003 0.0002 0.0005 0 0 0.0039 0.0004 0.0001 0.0004 0.0001 0.0002
Lipid Particles 0.0092 0.0016 0.001 0.0002 0.0136 0.0031 0.0068 0.0036 0.0028 0.0068 0.0012 0.0008 0.0106 0.0022 0.0051 0.0014 0.003 0.0045
Mitochondria 0.9041 0.9796 0.9723 0.998 0.9581 0.9803 0.9533 0.9557 0.957 0.9541 0.9928 0.9962 0.9269 0.9887 0.9735 0.9783 0.9768 0.9869
None 0.0017 0 0 0 0.0004 0.0058 0.0021 0 0 0 0 0 0.0009 0 0.0005 0 0.0012 0
Nuclear Periphery 0.0053 0 0 0 0.0002 0 0.0001 0.0001 0 0 0 0 0.0011 0 0 0.0003 0.0001 0
Nucleolus 0.007 0.0005 0.0009 0 0.0045 0.0012 0.0019 0.0026 0.0127 0.0002 0 0 0.0044 0.0005 0.0064 0 0.0044 0.0001
Nucleus 0.0091 0.0001 0.0001 0 0.0004 0.0001 0.0003 0.0002 0.0005 0 0 0 0.0014 0 0.0002 0 0.0003 0
Peroxisomes 0.0049 0.0012 0.0023 0.0004 0.0044 0.0009 0.0079 0.003 0.0025 0.0126 0.0021 0.0003 0.0112 0.0006 0.0025 0.0001 0.0028 0.0003
Punctate Nuclear 0.0046 0 0 0 0.0001 0 0.0005 0 0 0 0 0 0.0013 0 0.0001 0 0 0
Vacuole 0.0013 0.0008 0.0005 0 0.0011 0.0003 0.0003 0.0038 0.0014 0.0001 0 0.0001 0.0006 0.0003 0.0003 0.0002 0.0006 0.0001
Vacuole Periphery 0.0035 0.0132 0.0027 0.0008 0.0044 0.0021 0.0015 0.0156 0.0061 0.0016 0.0002 0.0018 0.0031 0.0063 0.0027 0.0185 0.0019 0.0074

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 345.0818 323.4272 317.3419 301.6298 294.5705 388.1628 428.5316 389.1267 412.9307 343.8615
Translational Efficiency 1.0792 1.114 1.1041 1.27 1.0936 1.0182 1.0423 1.0391 1.025 1.0762

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2059 1140 1985 69 2331 1292 188 2832 4390 2432 2173 2901

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3416.03 3163.32 3836.37 2466.71 2910.51 3396.64 3011.26 2828.51 3147.61 3287.27 3764.98 2819.90
Standard Deviation 1172.84 1050.78 1117.51 783.83 956.59 1039.44 688.85 770.10 1093.02 1051.24 1111.60 772.40
Intensity Change Log 2 — -0.110881 0.167421 -0.469733 — 0.222836 0.049095 -0.041230 — 0.052270 0.114188 -0.256726

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003143 0.011736 0.004610 0.014341 0.003345 0.007245 0.010843 0.001267 0.003251 0.009350 0.005149 0.001578
Bud Neck 0.000540 0.002503 0.002144 0.006964 0.000331 0.001970 0.004991 0.000058 0.000429 0.002220 0.002391 0.000223
Bud Site 0.000287 0.001808 0.000941 0.017316 0.000243 0.001106 0.005804 0.000162 0.000264 0.001435 0.001362 0.000570
Cell Periphery 0.000033 0.000077 0.000063 0.000934 0.000036 0.000077 0.000160 0.000120 0.000035 0.000077 0.000072 0.000139
Cytoplasm 0.000129 0.000437 0.000161 0.000644 0.000131 0.000556 0.000375 0.000107 0.000130 0.000500 0.000179 0.000120
Cytoplasmic Foci 0.015645 0.041158 0.020095 0.023928 0.013967 0.038149 0.060597 0.000957 0.014754 0.039559 0.023599 0.001504
Eisosomes 0.004011 0.008828 0.002399 0.004443 0.004236 0.003945 0.002182 0.000700 0.004130 0.006234 0.002380 0.000789
Endoplasmic Reticulum 0.000044 0.000119 0.000054 0.000889 0.000038 0.000089 0.000103 0.000024 0.000041 0.000103 0.000058 0.000044
Endosome 0.000188 0.000684 0.000341 0.001757 0.000095 0.000660 0.001144 0.000282 0.000138 0.000671 0.000411 0.000317
Golgi 0.005418 0.013476 0.010288 0.043919 0.006032 0.017838 0.014595 0.004391 0.005744 0.015793 0.010661 0.005331
Lipid Particles 0.005731 0.009775 0.011483 0.004816 0.004480 0.010605 0.007640 0.001059 0.005067 0.010216 0.011151 0.001148
Mitochondria 0.939988* 0.850225* 0.908860* 0.807081* 0.945747* 0.873578* 0.824162* 0.985385* 0.943046* 0.862632* 0.901532* 0.981144*
Mitotic Spindle 0.000307 0.000652 0.002784 0.010757 0.000215 0.001359 0.013974 0.000132 0.000259 0.001028 0.003752 0.000384
None 0.005708 0.003943 0.006099 0.005941 0.005489 0.005921 0.003952 0.001468 0.005592 0.004993 0.005913 0.001574
Nuclear Periphery 0.000314 0.000312 0.001622 0.001482 0.000190 0.000780 0.003491 0.000052 0.000249 0.000560 0.001784 0.000086
Nuclear Periphery Foci 0.000101 0.000227 0.000327 0.000649 0.000078 0.000376 0.000646 0.000019 0.000089 0.000306 0.000355 0.000034
Nucleolus 0.000855 0.004363 0.001744 0.001363 0.000652 0.002944 0.002893 0.000029 0.000747 0.003609 0.001843 0.000061
Nucleus 0.000042 0.000190 0.000073 0.000148 0.000040 0.000243 0.000146 0.000013 0.000041 0.000218 0.000079 0.000016
Peroxisomes 0.010529 0.041053 0.008405 0.012732 0.011154 0.014285 0.020508 0.000965 0.010861 0.026833 0.009452 0.001245
Vacuole 0.001711 0.004579 0.004594 0.013236 0.001221 0.008652 0.008324 0.000572 0.001451 0.006743 0.004916 0.000874
Vacuole Periphery 0.005275 0.003853 0.012912 0.026660 0.002279 0.009622 0.013468 0.002238 0.003684 0.006918 0.012960 0.002819

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.10 -1.93 -2.51 -0.56 -2.16 -3.16 -2.22 3.40 5.40 2.88 -6.55 -2.61 3.84 8.85 5.35
Bud Neck -7.07 -5.09 -2.86 -1.97 -2.11 -5.70 -5.23 5.34 6.74 5.54 -8.92 -6.64 2.90 9.77 7.27
Bud Site -4.12 -3.25 -1.22 -1.11 -1.17 -3.43 -1.54 0.75 3.92 1.56 -5.36 -3.02 -0.89 2.19 1.67
Cell Periphery -4.53 -3.44 -1.84 -1.75 -1.77 -2.09 -1.99 -2.82 -1.43 0.62 -3.71 -3.17 -3.61 -2.12 -2.30
Cytoplasm -6.56 -1.17 -1.38 -0.55 -1.29 -1.40 -2.56 0.28 1.44 2.32 -2.21 -2.01 0.11 2.11 0.83
Cytoplasmic Foci -8.13 -2.60 -1.32 2.53 -0.60 -8.81 -4.40 11.81 14.71 5.66 -11.98 -5.20 16.11 19.70 14.39
Eisosomes -6.40 5.18 -0.33 3.01 -1.59 0.57 4.29 12.48 7.37 3.64 -4.71 7.45 15.57 13.15 9.73
Endoplasmic Reticulum -5.53 -0.95 -2.56 -2.33 -2.53 -3.88 -3.25 1.36 6.97 4.47 -6.60 -1.62 -0.33 5.05 1.11
Endosome -2.98 -3.02 -3.51 -2.26 -3.16 -5.18 -4.88 -1.47 2.28 3.47 -5.48 -6.35 -1.43 2.27 0.74
Golgi -5.75 -5.08 -2.98 -2.34 -2.60 -6.98 -2.65 1.77 7.97 3.16 -9.02 -5.35 0.50 8.32 4.89
Lipid Particles -2.83 -4.70 0.53 2.48 3.58 -5.20 -1.16 5.31 8.54 2.45 -5.62 -5.83 6.95 10.19 9.80
Mitochondria 11.58 6.29 4.35 1.38 3.31 10.77 5.94 -12.94 -17.88 -7.92 15.79 9.18 -14.86 -24.08 -18.32
Mitotic Spindle -2.30 -3.82 -1.19 -1.15 -0.90 -1.84 -2.39 0.74 1.98 2.41 -2.28 -4.52 -0.55 1.62 4.20
None 4.89 -1.09 -0.19 -1.61 0.13 -1.00 2.69 12.44 13.74 4.97 2.10 -1.06 17.14 14.95 17.51
Nuclear Periphery 0.03 -5.16 -2.49 -2.48 0.27 -4.83 -1.70 5.04 6.09 1.77 -4.22 -5.40 5.91 6.64 5.98
Nuclear Periphery Foci -3.63 -6.02 -2.78 -2.11 -1.60 -4.49 -3.80 4.53 5.43 4.20 -5.60 -7.32 4.88 6.98 8.78
Nucleolus -3.33 -3.32 -0.94 2.58 0.70 -3.44 -2.77 6.71 4.41 3.56 -4.71 -4.80 7.62 5.90 8.40
Nucleus -4.42 -3.29 -2.88 0.86 -1.98 -2.32 -4.12 2.14 2.62 5.28 -3.57 -4.05 2.49 4.03 5.74
Peroxisomes -9.13 1.36 -0.30 3.60 -0.59 -1.55 -1.76 9.11 7.71 3.75 -8.32 1.06 11.26 14.44 7.83
Vacuole -4.35 -5.24 -2.43 -1.81 -1.81 -6.16 -4.90 2.45 6.72 5.36 -7.43 -6.87 2.31 8.07 7.71
Vacuole Periphery 1.62 -5.93 -2.74 -2.93 -1.75 -7.59 -3.48 0.09 7.43 3.48 -4.90 -8.44 1.66 5.96 9.09
Standard name
Human Ortholog
Description Mitochondrial NADP-specific isocitrate dehydrogenase; catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Idp1

Idp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Idp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available