Standard name
Human Ortholog
Description Subunit VIIa of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 1.0 0.98 0.96 0.96 0.81 – 0.77 0.71 0.74 0.6 0.62 0.99 1.0 1.0 0.97 0.92 0.9 0.93 0.89 0.87 0.93 0.88 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.05 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.23 0.06 0.39 – 0.38 0.52 0.42 0.61 0.59 0 0 0 0 0.06 0.09 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 3 4
Bud 1 1 4 2 3 1 – 9 14 6 7 17 0 1 0 0 0 0 2 0 0 0 9 10
Bud Neck 0 0 2 0 0 0 – 8 1 2 0 7 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 0 – 2 0 0 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 2 2 4 – 0 4 1 1 2 0 0 1 0 1 3 1 0 0 0 0 0
Cytoplasm 335 53 186 387 480 334 – 559 360 356 203 243 248 353 469 61 58 73 324 71 170 120 302 287
Endoplasmic Reticulum 0 0 1 0 0 2 – 0 0 0 1 0 0 0 0 3 3 7 0 0 0 0 0 1
Endosome 0 1 0 0 7 1 – 1 0 0 0 1 0 0 1 1 0 1 1 2 1 0 1 7
Golgi 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 1 3
Mitochondria 14 0 8 93 30 162 – 274 265 202 206 230 2 0 1 0 4 7 4 1 8 2 6 10
Nucleus 0 0 0 0 1 0 – 0 2 1 2 1 0 1 0 0 2 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 – 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 1 5 2 – 29 4 12 3 6 0 0 0 0 0 0 2 0 1 0 3 6
Vac/Vac Membrane 2 0 0 1 1 3 – 4 4 2 2 2 0 0 0 1 2 2 0 0 6 2 6 8
Unique Cell Count 346 53 189 405 499 411 727 510 482 337 391 250 354 470 63 63 81 348 81 196 129 345 351
Labelled Cell Count 352 55 201 486 530 509 890 655 582 426 511 250 355 472 66 71 93 348 81 196 129 345 351


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 5.0 3.3 3.4 3.8 2.8 – 2.9 2.4 2.7 2.4 2.4 4.5 4.4 4.3 6.0 5.8 6.3 4.3 4.9 4.9
Std Deviation (1e-4) 0.5 1.4 0.6 0.9 1.6 1.0 – 1.1 0.8 1.0 0.6 0.9 1.0 1.1 1.3 2.2 1.5 2.2 0.5 1.3 1.9
Intensity Change (Log2) – – – 0.05 0.22 -0.21 – -0.17 -0.45 -0.27 -0.42 -0.44 0.46 0.45 0.4 0.88 0.84 0.96 0.4 0.58 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.8 -1.5 -5.7 – -6.8 -7.9 -7.3 -9.6 -9.3 0.8 1.7 2.1 -0.8 -2.5 -3.2
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0628 0.2326 0.049 0.2944 -0.0884 0.1226 -0.8089 -0.059 -0.28 -0.2932 -0.1279 -0.2194 0.1896 1.5315 1.1594 0.9327 0.6442 1.1057
Actin 0.0247 0.0011 0.0062 0.0052 0.0089 0.0034 0.0033 0.0014 0.0181 0.0037 0.0128 0.0019 0.0187 0.0001 0.0037 0.0002 0.0001 0.0002
Bud 0.0004 0.016 0.0096 0.0002 0.0072 0.0017 0.0012 0.0023 0.0018 0.0013 0.0048 0.0016 0.0001 0 0.0001 0 0 0.0001
Bud Neck 0.0105 0.0004 0.0029 0.0018 0.0231 0.0018 0.0028 0.0003 0.0008 0.0003 0.0009 0.0028 0.0005 0 0.0001 0.0002 0.0002 0.0003
Bud Periphery 0.0005 0.0029 0.0048 0.0002 0.0043 0.0032 0.0021 0.0049 0.0033 0.0019 0.0101 0.0022 0.0001 0 0.0001 0 0 0.0002
Bud Site 0.0032 0.0279 0.0102 0.001 0.0029 0.0002 0.008 0.006 0.0041 0.0009 0.0051 0.0006 0.0002 0.0002 0.0001 0 0.0001 0
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0.0003 0.0001 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0 0 0 0 0.0001
Cytoplasm 0.0893 0.1792 0.1539 0.0917 0.1665 0.2238 0.1858 0.3689 0.2603 0.2496 0.2063 0.3508 0.1809 0.3437 0.2885 0.35 0.4452 0.4694
Cytoplasmic Foci 0.0175 0.008 0.0082 0.0107 0.0174 0.017 0.0128 0.0142 0.0128 0.0201 0.0314 0.0161 0.0055 0.0019 0.0032 0.0061 0.0039 0.004
Eisosomes 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0022 0.0008 0.0008 0.0008 0.0028 0.0007 0.0022 0.0018 0.0017 0.0032 0.0026 0.0014 0.0021 0.0007 0.0014 0.0019 0.0005 0.0034
Endosome 0.0101 0.0006 0.001 0.0088 0.0089 0.0038 0.0111 0.0024 0.0076 0.0119 0.0422 0.0055 0.0019 0.0003 0.0034 0.0023 0.0006 0.0014
Golgi 0.0037 0.0002 0.0003 0.0028 0.0016 0.0008 0.0018 0.0003 0.0027 0.0009 0.0159 0.0011 0.0013 0 0.0008 0.0001 0 0.0001
Lipid Particles 0.0105 0.0002 0.0004 0.0063 0.0021 0.0016 0.0064 0.0006 0.0018 0.0017 0.0259 0.0017 0.002 0 0.001 0.0001 0.0001 0.0001
Mitochondria 0.0034 0.0004 0.0016 0.0011 0.0007 0.0017 0.0031 0.0015 0.0097 0.0012 0.0299 0.0033 0.0002 0.0001 0.0004 0.0002 0.0001 0.0002
None 0.7843 0.7491 0.7844 0.8304 0.7277 0.7194 0.7371 0.5846 0.6593 0.6674 0.5685 0.591 0.7806 0.65 0.6915 0.634 0.5462 0.5145
Nuclear Periphery 0.0049 0.0006 0.001 0.0025 0.0025 0.0012 0.0023 0.0011 0.0014 0.0074 0.0037 0.0019 0.0007 0.0003 0.0024 0.0008 0.0002 0.0008
Nucleolus 0.001 0.0006 0.0007 0.0011 0.0025 0.0003 0.0014 0.0005 0.0007 0.0007 0.0014 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0001
Nucleus 0.003 0.0048 0.005 0.0029 0.0071 0.0018 0.0032 0.0036 0.0041 0.0073 0.0044 0.0044 0.0006 0.0017 0.0014 0.0015 0.0016 0.0033
Peroxisomes 0.0214 0.0005 0.0028 0.0025 0.0012 0.0016 0.0029 0.0008 0.0035 0.0038 0.0172 0.0019 0.0028 0 0.0002 0.0001 0 0.0001
Punctate Nuclear 0.0073 0.0058 0.0054 0.0292 0.0098 0.0152 0.0088 0.0032 0.0041 0.0142 0.0062 0.0096 0.0008 0.0004 0.0009 0.0016 0.0007 0.001
Vacuole 0.001 0.0004 0.0005 0.0004 0.0019 0.0004 0.0026 0.001 0.0015 0.0019 0.0073 0.0011 0.0005 0.0003 0.0005 0.0006 0.0002 0.0009
Vacuole Periphery 0.0006 0.0001 0.0001 0.0002 0.0004 0.0002 0.0008 0.0002 0.0003 0.0006 0.0031 0.0003 0.0001 0 0.0002 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 85.8203 98.6376 89.4586 85.9137 95.3741 55.1397 88.651 72.922 113.2181 75.2835
Translational Efficiency 3.7613 2.841 2.9176 4.5804 3.9806 6.4932 3.0626 3.8398 2.5119 3.5355

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
18 1559 595 1046 1941 935 51 1411 1959 2494 646 2457

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 893.24 735.68 858.92 803.87 689.95 745.31 980.74 801.45 691.82 739.29 868.54 802.48
Standard Deviation 310.23 96.55 120.46 117.11 81.38 94.02 142.41 108.07 88.44 95.72 126.67 112.02
Intensity Change Log 2 — -0.279969 -0.056524 -0.152086 — 0.111349 0.507379 0.216121 — -0.096273 0.216605 0.020027

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001361 0.000315 0.000122 0.001615 0.000125 0.000171 0.004434 0.001713 0.000136 0.000261 0.000462 0.001671
Bud Neck 0.008671 0.027476 0.000887 0.010925 0.014437 0.035456 0.006260 0.004808 0.014384 0.030468 0.001311 0.007412
Bud Site 0.011707 0.011474 0.001644 0.030189 0.003852 0.011022 0.239033* 0.028502 0.003924 0.011305 0.020385 0.029220
Cell Periphery 0.000612 0.000120 0.000090 0.000233 0.000128 0.000199 0.000297 0.000163 0.000133 0.000150 0.000107 0.000193
Cytoplasm 0.331145* 0.462247* 0.571034* 0.431367* 0.477640* 0.380845* 0.101777* 0.474953* 0.476294* 0.431729* 0.533988* 0.456397*
Cytoplasmic Foci 0.100831 0.228010* 0.014968 0.032440 0.237742* 0.250400* 0.022024 0.033348 0.236484* 0.236404* 0.015525 0.032961
Eisosomes 0.000178 0.000123 0.000021 0.000070 0.000195 0.000191 0.000102 0.000056 0.000195 0.000148 0.000028 0.000062
Endoplasmic Reticulum 0.011940 0.000580 0.001496 0.002877 0.000643 0.001411 0.008949 0.001286 0.000746 0.000892 0.002085 0.001963
Endosome 0.004261 0.020024 0.001510 0.010832 0.009405 0.025395 0.029428 0.008826 0.009358 0.022038 0.003714 0.009680
Golgi 0.000442 0.003431 0.000071 0.008072 0.002154 0.004383 0.000669 0.003603 0.002138 0.003788 0.000118 0.005506
Lipid Particles 0.006260 0.006268 0.000916 0.002059 0.009786 0.013133 0.008610 0.001476 0.009754 0.008842 0.001524 0.001724
Mitochondria 0.001657 0.007893 0.000158 0.009412 0.005299 0.010666 0.001411 0.006885 0.005266 0.008933 0.000257 0.007961
Mitotic Spindle 0.000626 0.003877 0.000573 0.040253 0.002418 0.004525 0.001333 0.024985 0.002402 0.004120 0.000633 0.031485
None 0.002469 0.003184 0.005575 0.005445 0.002097 0.004560 0.000170 0.009373 0.002101 0.003700 0.005148 0.007701
Nuclear Periphery 0.006717 0.000436 0.000614 0.001822 0.000409 0.000732 0.001931 0.001365 0.000467 0.000547 0.000718 0.001560
Nuclear Periphery Foci 0.004054 0.000418 0.001566 0.001332 0.000264 0.000313 0.010382 0.000599 0.000299 0.000379 0.002262 0.000911
Nucleolus 0.014479 0.002178 0.000249 0.001981 0.000945 0.003235 0.000964 0.000687 0.001070 0.002574 0.000305 0.001238
Nucleus 0.416562* 0.134283* 0.366730* 0.270760* 0.181873* 0.154746* 0.073726 0.288857* 0.184030* 0.141955* 0.343599* 0.281153*
Peroxisomes 0.002076 0.012315 0.000122 0.002324 0.005977 0.008671 0.001618 0.003406 0.005941 0.010949 0.000240 0.002945
Vacuole 0.070034 0.073682 0.031545 0.130814 0.043938 0.087323 0.484343* 0.102247 0.044178 0.078796 0.067292 0.114409
Vacuole Periphery 0.003917 0.001664 0.000107 0.005177 0.000671 0.002623 0.002541 0.002863 0.000701 0.002023 0.000299 0.003848

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.24 1.36 0.83 -2.07 -2.75 -1.25 -2.58 -2.34 -2.20 2.16 -3.19 -2.54 -3.31 -2.77 -1.06
Bud Neck -4.08 2.01 0.41 10.10 -6.43 -6.49 3.99 9.22 9.92 1.09 -8.65 13.01 7.82 14.96 -8.51
Bud Site 0.08 1.21 -0.52 -2.19 -6.60 -3.60 -4.08 -5.87 -2.05 3.76 -6.61 -3.04 -7.77 -3.07 -0.39
Cell Periphery 2.31 2.41 2.13 -2.71 -4.15 -4.88 -4.21 0.71 5.45 4.37 -1.86 1.83 -0.42 1.53 -2.31
Cytoplasm -2.34 -3.66 -0.57 10.11 14.46 9.42 19.86 8.88 -1.16 -14.78 5.77 -2.92 12.75 7.10 11.14
Cytoplasmic Foci -5.39 3.39 3.07 36.30 -3.71 -1.54 39.03 42.60 31.42 -2.45 -0.00 44.89 43.64 47.86 -5.41
Eisosomes 1.80 4.67 3.25 4.92 -13.50 0.36 4.39 14.47 13.80 4.01 4.26 17.75 14.19 11.44 -11.57
Endoplasmic Reticulum 1.79 1.57 1.27 -12.59 -5.87 -1.26 -5.00 -12.66 -0.97 4.35 -0.65 -7.14 -13.99 -7.04 -0.27
Endosome -9.56 2.61 -0.85 9.96 -4.96 -8.52 -2.53 4.56 10.51 3.39 -10.76 8.03 5.12 14.52 -2.84
Golgi -5.96 1.57 -2.32 0.27 -2.87 -3.04 3.44 0.93 3.10 -1.36 -3.92 9.34 -0.12 2.34 -3.81
Lipid Particles 0.10 2.91 2.37 8.79 -4.68 -2.22 1.09 12.24 8.74 3.40 1.08 11.68 11.99 12.17 -0.77
Mitochondria -6.33 2.63 -2.24 2.64 -3.73 -3.22 4.48 1.46 4.28 -2.21 -3.05 5.60 1.38 4.94 -4.68
Mitotic Spindle -2.37 -0.92 -7.09 -6.11 -6.29 -1.37 -1.38 -5.85 -4.60 -0.45 -1.51 -0.49 -8.60 -7.70 -7.09
None -0.85 -2.71 -1.53 -1.13 1.61 -2.53 8.64 -3.90 -0.67 -5.94 -3.22 -5.76 -4.71 -1.62 -0.39
Nuclear Periphery 1.52 1.43 0.76 -18.77 -16.18 -1.77 -3.76 -19.51 -7.17 0.57 -1.18 -5.83 -24.06 -19.18 -16.67
Nuclear Periphery Foci 1.74 1.01 1.20 -5.35 1.24 -1.01 -3.89 -8.83 -5.69 3.73 -2.06 -6.92 -8.24 -7.16 4.59
Nucleolus 1.17 1.40 1.26 1.41 -2.42 -2.93 1.10 2.34 3.45 0.62 -4.14 4.78 0.43 4.16 -3.06
Nucleus 4.07 0.47 1.40 -18.92 4.59 3.86 3.68 -17.20 -17.96 -9.25 7.94 -15.05 -19.98 -27.18 2.77
Peroxisomes -8.72 3.13 1.50 10.94 -3.77 -2.32 7.19 5.86 6.47 -0.71 -5.28 10.43 7.27 12.19 -4.38
Vacuole 0.82 1.42 -2.77 -19.09 -20.90 -9.35 -11.93 -23.43 -13.92 8.12 -12.39 -5.67 -32.45 -23.94 -14.61
Vacuole Periphery 0.98 1.80 0.71 -0.67 -3.09 -6.38 -2.27 -1.46 2.42 0.53 -7.78 7.28 -2.53 0.89 -3.77
Standard name
Human Ortholog
Description Subunit VIIa of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain
Localization
Cell Percentages cytoplasm (24%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cox9

Cox9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cox9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available