Standard name
Human Ortholog
Description Component of the Rpd3L histone deacetylase complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0.06 0.06 0 0 0 0 0 0.08 0.12 0.78 0.72 0.7 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.18 0 0 0 0 0.06 0 0.13 0.06 0.15 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.88 0.87 0.85 0.89 0.91 0.87 0.78 0.83 0.65 0.73 0.68 0.73 0.89 0.89 0.79 0.05 0.08 0.11 0.87 0.85 0.77 0.8 0.83 0.71
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.27 0.17 0.18 0.23 0.07 0.16 0.22 0.24 0.45 0.4 0.47 0.51 0.26 0.11 0.13 0 0 0 0.12 0.07 0.14 0.13 0.11 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.23 0.25 0.33 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Bud 0 0 2 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 5 0 0 0 0
Bud Neck 0 0 0 1 0 1 0 1 1 0 3 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 2 2 5 3 3 2 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 7 3 3 5 8 10 16 10 8 6 1 3 28 46 104 97 86 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 10 5 3 0 0 0 0 0 2
Endosome 0 1 1 0 0 0 0 0 0 0 0 0 0 0 4 4 8 2 0 1 2 1 0 2
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Mitochondria 2 44 1 4 0 6 10 4 32 13 27 9 4 6 10 4 1 3 0 4 1 0 0 1
Nucleus 116 209 131 87 97 167 137 235 159 162 122 77 131 327 307 7 11 13 116 207 140 140 178 147
Nuclear Periphery 0 1 0 0 0 1 2 2 3 0 0 1 1 1 2 0 1 1 0 0 0 0 0 0
Nucleolus 36 40 27 23 7 31 39 67 111 88 84 54 38 42 49 0 0 0 15 17 25 23 23 37
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 1 0 0 3 3 5 0 2 1 0 3 5 12 4 1 1 0 0 0 1 1 2
Vac/Vac Membrane 1 4 4 0 0 2 5 1 5 3 6 1 0 1 4 31 34 41 0 5 5 6 4 6
Unique Cell Count 132 239 154 98 107 193 175 284 244 222 179 106 147 369 391 134 134 123 134 244 181 176 215 208
Labelled Cell Count 156 307 171 119 109 221 209 333 326 280 253 145 180 410 435 166 158 150 134 244 181 176 215 208


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 5.9 8.1 7.3 9.1 7.7 7.4 7.7 6.7 7.0 6.1 6.6 7.5 7.4 6.9 5.3 6.5 6.5 7.8 8.5 8.8
Std Deviation (1e-4) 1.6 1.1 1.8 1.6 2.7 2.9 2.8 2.2 2.4 2.3 2.2 2.3 1.9 1.8 2.0 1.0 1.1 1.3 1.6 2.1 2.5
Intensity Change (Log2) – – – -0.16 0.16 -0.08 -0.14 -0.06 -0.28 -0.21 -0.4 -0.3 -0.11 -0.13 -0.23 -0.61 -0.32 -0.31 -0.05 0.08 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 1.7 1.8 1.2 0 0 0 0 2.5 3.6 13.3 12.5 12.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.6 0 4.4 2.6 4.7 0 0 0 0 0 0 0
Nucleus 0.8 1.3 0.4 -1.6 -0.6 -4.3 -2.8 -3.6 -2.5 1.0 1.1 -1.7 -13.5 -13.0 -12.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.2 -2.6 -0.4 1.1 1.5 5.7 4.6 5.7 5.7 1.8 -1.9 -1.5 -5.1 -5.1 -4.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 5.3 5.7 6.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.3714 6.7924 6.9072 6.1937 6.4806 6.6062 4.8806 5.8113 4.7495 4.8715 4.0861 4.9382 3.3818 2.717 2.5209 2.656 2.5826 2.2928
Actin 0.0349 0 0.0048 0 0.0004 0.0001 0.0804 0 0.0147 0.0465 0.0576 0.0006 0 0 0 0 0 0
Bud 0.0004 0 0.0001 0 0.0004 0 0.0012 0.0003 0.0002 0.0004 0.003 0.0001 0 0 0 0 0 0.0006
Bud Neck 0.0015 0 0.0002 0.0002 0.0018 0.001 0.0017 0.0006 0.0002 0.0003 0.0016 0.0009 0.0001 0 0 0 0.0005 0.0009
Bud Periphery 0.0008 0 0.0002 0.0001 0.0011 0.0001 0.0019 0.0005 0.0004 0.0004 0.0059 0.0001 0 0 0 0 0.0001 0.0004
Bud Site 0.0059 0 0.0003 0.0001 0.0022 0.0001 0.0029 0.0068 0.0004 0.0007 0.0064 0.0001 0.0001 0 0 0 0.0001 0.0001
Cell Periphery 0.0005 0 0.0002 0 0.0003 0 0.0004 0.0001 0.0001 0 0.0003 0 0 0 0 0 0 0.0001
Cytoplasm 0.002 0 0.0006 0 0.0004 0 0.0322 0.0001 0.0015 0.0023 0.0008 0.0003 0 0 0 0 0 0.0008
Cytoplasmic Foci 0.0137 0 0.0007 0 0.0078 0.0048 0.0153 0.001 0.003 0.0095 0.0235 0.0013 0 0 0 0 0 0.0014
Eisosomes 0.0005 0 0 0 0 0 0.0012 0 0.0001 0.0001 0.0002 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0025 0 0.0004 0 0.0004 0 0.007 0 0.0006 0.0037 0.0009 0.0001 0 0 0 0 0 0
Endosome 0.0108 0 0.0011 0 0.0018 0.0006 0.0408 0.0011 0.0041 0.0249 0.033 0.0027 0 0 0 0 0 0
Golgi 0.0034 0 0.0005 0 0.0006 0.0004 0.01 0.0004 0.0021 0.0139 0.0395 0.0008 0 0 0 0 0 0
Lipid Particles 0.0226 0 0.0005 0 0.0035 0.0015 0.018 0.0004 0.0125 0.0068 0.0482 0.0053 0 0 0 0 0 0
Mitochondria 0.0031 0 0.0037 0.0003 0.003 0.0057 0.0112 0.0002 0.0044 0.0044 0.0351 0.0009 0.0003 0.0001 0.0001 0 0.0002 0.0027
None 0.004 0 0.0009 0 0.0001 0.0001 0.0349 0 0.0138 0.0007 0.0008 0.0002 0 0 0 0 0 0.0009
Nuclear Periphery 0.0127 0.0002 0.0029 0.0009 0.0013 0.0002 0.0423 0.0003 0.0036 0.0027 0.004 0.0059 0.0001 0.0001 0.0001 0.0001 0.0006 0.0001
Nucleolus 0.0655 0.0238 0.0274 0.0549 0.1486 0.1438 0.0298 0.0349 0.0205 0.017 0.1135 0.1141 0.0414 0.0284 0.0328 0.0198 0.24 0.128
Nucleus 0.7884 0.9755 0.9444 0.9425 0.7934 0.8375 0.6406 0.9494 0.9105 0.858 0.5782 0.8581 0.9578 0.9713 0.9666 0.9796 0.7554 0.8621
Peroxisomes 0.0052 0 0.0041 0 0.0267 0.0002 0.0076 0.0002 0.0012 0.0051 0.0295 0.0009 0 0 0 0 0 0.0001
Punctate Nuclear 0.0193 0.0004 0.0045 0.0006 0.0023 0.0033 0.0143 0.0022 0.004 0.0018 0.0124 0.0073 0.0001 0.0001 0.0002 0.0003 0.0028 0.0011
Vacuole 0.0013 0 0.0013 0.0001 0.0034 0.0002 0.003 0.0012 0.0009 0.0003 0.0039 0.0002 0.0001 0 0 0 0.0001 0.0003
Vacuole Periphery 0.0009 0 0.0012 0.0001 0.0006 0.0002 0.0032 0.0001 0.0009 0.0004 0.0016 0.0002 0 0 0 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.0655 18.9473 14.0597 21.3944 25.8642 11.2149 26.2009 24.7193 19.8512 26.3153
Translational Efficiency 0.7235 1.1272 1.2799 0.769 0.6978 1.746 0.7312 0.9999 0.9592 0.7773

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1598 1891 2321 1800 1555 1397 410 1358 3153 3288 2731 3158

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 753.34 989.64 1017.14 987.28 722.58 832.82 1069.35 1005.76 738.17 923.01 1024.98 995.23
Standard Deviation 145.19 156.46 142.56 144.91 121.31 137.94 140.03 148.21 134.83 167.85 143.40 146.62
Intensity Change Log 2 — 0.393603 0.433145 0.390158 — 0.204847 0.565505 0.477057 — 0.304273 0.499463 0.433356

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000465 0.000478 0.000739 0.002271 0.000618 0.000557 0.001366 0.001092 0.000540 0.000511 0.000833 0.001764
Bud Neck 0.004444 0.013974 0.030057 0.048887 0.006087 0.006840 0.016637 0.033840 0.005255 0.010943 0.028042 0.042417
Bud Site 0.000725 0.000880 0.001928 0.014129 0.001091 0.001176 0.001598 0.004957 0.000905 0.001006 0.001878 0.010185
Cell Periphery 0.000133 0.000049 0.000043 0.000182 0.000166 0.000164 0.000041 0.000117 0.000149 0.000098 0.000043 0.000154
Cytoplasm 0.003593 0.001096 0.000370 0.007530 0.004028 0.002205 0.000201 0.004343 0.003807 0.001568 0.000345 0.006159
Cytoplasmic Foci 0.000646 0.000057 0.000012 0.002905 0.001138 0.002165 0.000036 0.001147 0.000888 0.000952 0.000016 0.002149
Eisosomes 0.000032 0.000052 0.000061 0.000049 0.000036 0.000040 0.000073 0.000044 0.000034 0.000047 0.000063 0.000047
Endoplasmic Reticulum 0.002539 0.001797 0.001684 0.005596 0.003280 0.002940 0.003038 0.003672 0.002904 0.002283 0.001887 0.004768
Endosome 0.000208 0.000118 0.000102 0.005904 0.000356 0.000877 0.000100 0.000867 0.000281 0.000440 0.000101 0.003738
Golgi 0.000431 0.000155 0.000122 0.003117 0.000691 0.000900 0.000198 0.001863 0.000559 0.000471 0.000133 0.002578
Lipid Particles 0.000226 0.000039 0.000021 0.000449 0.000532 0.001063 0.000069 0.000089 0.000377 0.000474 0.000029 0.000294
Mitochondria 0.001336 0.000836 0.002031 0.008289 0.001411 0.002546 0.001274 0.004305 0.001373 0.001562 0.001917 0.006576
Mitotic Spindle 0.001206 0.001417 0.001670 0.053837 0.001544 0.001632 0.003878 0.013856 0.001373 0.001508 0.002001 0.036644
None 0.018320 0.001581 0.001146 0.005419 0.013131 0.010665 0.002894 0.005550 0.015761 0.005441 0.001409 0.005475
Nuclear Periphery 0.000115 0.000087 0.000246 0.007647 0.000173 0.000226 0.000122 0.000875 0.000144 0.000146 0.000228 0.004735
Nuclear Periphery Foci 0.000667 0.000156 0.000066 0.002897 0.000955 0.000794 0.000107 0.000787 0.000809 0.000427 0.000072 0.001990
Nucleolus 0.088688 0.119185 0.092074 0.032472 0.093059 0.098049 0.098188 0.045775 0.090843 0.110205 0.092992 0.038193
Nucleus 0.875081* 0.856895* 0.866238* 0.780086* 0.870052* 0.863922* 0.867074* 0.873278* 0.872601* 0.859881* 0.866364* 0.820160*
Peroxisomes 0.000729 0.000657 0.000693 0.001045 0.001024 0.001487 0.002254 0.000626 0.000874 0.001010 0.000927 0.000865
Vacuole 0.000256 0.000258 0.000395 0.012425 0.000368 0.000960 0.000673 0.002354 0.000312 0.000556 0.000436 0.008094
Vacuole Periphery 0.000161 0.000233 0.000301 0.004862 0.000261 0.000792 0.000179 0.000564 0.000210 0.000471 0.000283 0.003014

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.26 -7.55 -6.04 -6.05 -4.64 1.07 -10.23 -11.47 -13.76 2.35 0.88 -9.77 -8.08 -8.39 -5.33
Bud Neck -14.17 -25.94 -23.08 -17.34 -7.47 -0.96 -9.94 -18.64 -17.59 -9.31 -10.95 -26.66 -29.44 -24.24 -8.94
Bud Site -1.15 -6.65 -8.77 -8.66 -7.30 -0.32 -5.63 -6.55 -6.30 -4.85 -0.74 -6.91 -10.59 -10.45 -8.43
Cell Periphery 4.19 4.48 -0.75 -9.10 -10.56 0.09 5.66 2.29 1.53 -5.82 2.67 7.14 1.05 -2.36 -11.25
Cytoplasm 2.77 4.06 -4.23 -8.21 -10.42 1.81 4.24 -0.37 -2.32 -4.91 3.31 5.68 -3.36 -7.71 -10.80
Cytoplasmic Foci 1.95 2.10 -1.30 -3.74 -3.89 -1.14 2.79 1.22 2.24 -2.53 0.06 3.52 -0.06 -0.13 -4.67
Eisosomes -10.21 -15.38 -10.41 -0.50 4.47 -1.17 -12.07 -6.61 -4.42 8.32 -7.97 -17.99 -11.99 -3.19 7.57
Endoplasmic Reticulum 4.93 4.50 -12.09 -14.74 -14.46 1.23 -1.16 -5.32 -6.00 -3.55 4.15 5.64 -12.66 -15.43 -16.55
Endosome 2.12 2.81 -4.83 -4.98 -5.00 -2.40 4.30 -2.07 0.54 -3.52 -1.66 5.08 -5.14 -4.52 -5.67
Golgi 1.31 1.39 -2.12 -3.00 -3.04 -0.47 1.62 -0.78 -0.42 -1.82 0.45 2.33 -2.06 -2.44 -3.59
Lipid Particles 2.41 2.60 -1.77 -8.17 -8.89 -0.92 1.30 1.02 1.89 -3.09 -0.35 2.01 0.45 0.86 -10.19
Mitochondria 0.85 -1.38 -6.86 -10.93 -7.45 -1.53 -0.27 -3.32 -1.46 -3.67 -0.46 -1.74 -7.66 -7.85 -7.10
Mitotic Spindle -0.19 -1.20 -11.94 -11.97 -11.69 -0.13 -1.15 -6.34 -6.19 -4.81 -0.21 -1.58 -13.45 -13.39 -12.83
None 7.80 8.06 5.86 -6.26 -8.03 1.16 5.96 4.85 3.88 -1.76 6.98 10.65 7.59 0.45 -7.65
Nuclear Periphery 1.03 -3.24 -5.83 -5.85 -5.59 -1.14 -0.30 -5.87 -5.57 -5.86 -0.35 -2.77 -6.67 -6.66 -6.35
Nuclear Periphery Foci 3.28 3.90 -3.48 -5.72 -6.05 0.60 3.32 0.64 0.14 -2.06 2.37 5.04 -2.08 -4.15 -6.15
Nucleolus -7.27 -2.16 16.11 23.78* 18.85 -1.09 -1.31 12.24 13.26 8.54 -6.40 -2.18 20.39 26.73 19.89
Nucleus 3.47 3.65 10.02 8.08 7.99 0.91 1.52 2.24 1.38 0.37 3.16 3.93 9.48 7.29 6.40
Peroxisomes 0.71 -0.16 0.18 -0.80 0.54 -1.06 -4.86 2.07 2.10 8.06 -0.60 -0.95 1.66 1.81 4.28
Vacuole -0.12 -2.44 -9.31 -9.28 -9.13 -2.20 -2.87 -4.92 -4.37 -4.20 -1.95 -3.05 -10.40 -10.06 -10.09
Vacuole Periphery -1.89 -3.89 -4.81 -4.64 -4.50 -2.40 1.87 -2.11 1.38 -2.84 -2.69 -2.30 -5.04 -3.83 -4.75
Standard name
Human Ortholog
Description Component of the Rpd3L histone deacetylase complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rxt3

Rxt3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rxt3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available