Standard name | |
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Human Ortholog | |
Description | Component of the Rpd3L histone deacetylase complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 5 | 3 | 3 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 7 | 3 | 3 | 5 | 8 | 10 | 16 | 10 | 8 | 6 | 1 | 3 | 28 | 46 | 104 | 97 | 86 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 8 | 2 | 0 | 1 | 2 | 1 | 0 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Mitochondria | 2 | 44 | 1 | 4 | 0 | 6 | 10 | 4 | 32 | 13 | 27 | 9 | 4 | 6 | 10 | 4 | 1 | 3 | 0 | 4 | 1 | 0 | 0 | 1 |
Nucleus | 116 | 209 | 131 | 87 | 97 | 167 | 137 | 235 | 159 | 162 | 122 | 77 | 131 | 327 | 307 | 7 | 11 | 13 | 116 | 207 | 140 | 140 | 178 | 147 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 3 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 36 | 40 | 27 | 23 | 7 | 31 | 39 | 67 | 111 | 88 | 84 | 54 | 38 | 42 | 49 | 0 | 0 | 0 | 15 | 17 | 25 | 23 | 23 | 37 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 1 | 0 | 0 | 3 | 3 | 5 | 0 | 2 | 1 | 0 | 3 | 5 | 12 | 4 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 2 |
Vac/Vac Membrane | 1 | 4 | 4 | 0 | 0 | 2 | 5 | 1 | 5 | 3 | 6 | 1 | 0 | 1 | 4 | 31 | 34 | 41 | 0 | 5 | 5 | 6 | 4 | 6 |
Unique Cell Count | 132 | 239 | 154 | 98 | 107 | 193 | 175 | 284 | 244 | 222 | 179 | 106 | 147 | 369 | 391 | 134 | 134 | 123 | 134 | 244 | 181 | 176 | 215 | 208 |
Labelled Cell Count | 156 | 307 | 171 | 119 | 109 | 221 | 209 | 333 | 326 | 280 | 253 | 145 | 180 | 410 | 435 | 166 | 158 | 150 | 134 | 244 | 181 | 176 | 215 | 208 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.7 | 5.9 | 8.1 | 7.3 | 9.1 | 7.7 | 7.4 | 7.7 | 6.7 | 7.0 | 6.1 | 6.6 | 7.5 | 7.4 | 6.9 | 5.3 | 6.5 | 6.5 | 7.8 | 8.5 | 8.8 |
Std Deviation (1e-4) | 1.6 | 1.1 | 1.8 | 1.6 | 2.7 | 2.9 | 2.8 | 2.2 | 2.4 | 2.3 | 2.2 | 2.3 | 1.9 | 1.8 | 2.0 | 1.0 | 1.1 | 1.3 | 1.6 | 2.1 | 2.5 |
Intensity Change (Log2) | – | – | – | -0.16 | 0.16 | -0.08 | -0.14 | -0.06 | -0.28 | -0.21 | -0.4 | -0.3 | -0.11 | -0.13 | -0.23 | -0.61 | -0.32 | -0.31 | -0.05 | 0.08 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 1.7 | 1.8 | 1.2 | 0 | 0 | 0 | 0 | 2.5 | 3.6 | 13.3 | 12.5 | 12.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 2.6 | 0 | 4.4 | 2.6 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.8 | 1.3 | 0.4 | -1.6 | -0.6 | -4.3 | -2.8 | -3.6 | -2.5 | 1.0 | 1.1 | -1.7 | -13.5 | -13.0 | -12.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.2 | -2.6 | -0.4 | 1.1 | 1.5 | 5.7 | 4.6 | 5.7 | 5.7 | 1.8 | -1.9 | -1.5 | -5.1 | -5.1 | -4.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.3 | 5.7 | 6.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.3714 | 6.7924 | 6.9072 | 6.1937 | 6.4806 | 6.6062 | 4.8806 | 5.8113 | 4.7495 | 4.8715 | 4.0861 | 4.9382 | 3.3818 | 2.717 | 2.5209 | 2.656 | 2.5826 | 2.2928 |
Actin | 0.0349 | 0 | 0.0048 | 0 | 0.0004 | 0.0001 | 0.0804 | 0 | 0.0147 | 0.0465 | 0.0576 | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0004 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0012 | 0.0003 | 0.0002 | 0.0004 | 0.003 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0006 |
Bud Neck | 0.0015 | 0 | 0.0002 | 0.0002 | 0.0018 | 0.001 | 0.0017 | 0.0006 | 0.0002 | 0.0003 | 0.0016 | 0.0009 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0.0009 |
Bud Periphery | 0.0008 | 0 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0019 | 0.0005 | 0.0004 | 0.0004 | 0.0059 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0004 |
Bud Site | 0.0059 | 0 | 0.0003 | 0.0001 | 0.0022 | 0.0001 | 0.0029 | 0.0068 | 0.0004 | 0.0007 | 0.0064 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Cell Periphery | 0.0005 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 |
Cytoplasm | 0.002 | 0 | 0.0006 | 0 | 0.0004 | 0 | 0.0322 | 0.0001 | 0.0015 | 0.0023 | 0.0008 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0008 |
Cytoplasmic Foci | 0.0137 | 0 | 0.0007 | 0 | 0.0078 | 0.0048 | 0.0153 | 0.001 | 0.003 | 0.0095 | 0.0235 | 0.0013 | 0 | 0 | 0 | 0 | 0 | 0.0014 |
Eisosomes | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0012 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0025 | 0 | 0.0004 | 0 | 0.0004 | 0 | 0.007 | 0 | 0.0006 | 0.0037 | 0.0009 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0108 | 0 | 0.0011 | 0 | 0.0018 | 0.0006 | 0.0408 | 0.0011 | 0.0041 | 0.0249 | 0.033 | 0.0027 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.0034 | 0 | 0.0005 | 0 | 0.0006 | 0.0004 | 0.01 | 0.0004 | 0.0021 | 0.0139 | 0.0395 | 0.0008 | 0 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0226 | 0 | 0.0005 | 0 | 0.0035 | 0.0015 | 0.018 | 0.0004 | 0.0125 | 0.0068 | 0.0482 | 0.0053 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0031 | 0 | 0.0037 | 0.0003 | 0.003 | 0.0057 | 0.0112 | 0.0002 | 0.0044 | 0.0044 | 0.0351 | 0.0009 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0027 |
None | 0.004 | 0 | 0.0009 | 0 | 0.0001 | 0.0001 | 0.0349 | 0 | 0.0138 | 0.0007 | 0.0008 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0009 |
Nuclear Periphery | 0.0127 | 0.0002 | 0.0029 | 0.0009 | 0.0013 | 0.0002 | 0.0423 | 0.0003 | 0.0036 | 0.0027 | 0.004 | 0.0059 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 |
Nucleolus | 0.0655 | 0.0238 | 0.0274 | 0.0549 | 0.1486 | 0.1438 | 0.0298 | 0.0349 | 0.0205 | 0.017 | 0.1135 | 0.1141 | 0.0414 | 0.0284 | 0.0328 | 0.0198 | 0.24 | 0.128 |
Nucleus | 0.7884 | 0.9755 | 0.9444 | 0.9425 | 0.7934 | 0.8375 | 0.6406 | 0.9494 | 0.9105 | 0.858 | 0.5782 | 0.8581 | 0.9578 | 0.9713 | 0.9666 | 0.9796 | 0.7554 | 0.8621 |
Peroxisomes | 0.0052 | 0 | 0.0041 | 0 | 0.0267 | 0.0002 | 0.0076 | 0.0002 | 0.0012 | 0.0051 | 0.0295 | 0.0009 | 0 | 0 | 0 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0.0193 | 0.0004 | 0.0045 | 0.0006 | 0.0023 | 0.0033 | 0.0143 | 0.0022 | 0.004 | 0.0018 | 0.0124 | 0.0073 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0028 | 0.0011 |
Vacuole | 0.0013 | 0 | 0.0013 | 0.0001 | 0.0034 | 0.0002 | 0.003 | 0.0012 | 0.0009 | 0.0003 | 0.0039 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.0009 | 0 | 0.0012 | 0.0001 | 0.0006 | 0.0002 | 0.0032 | 0.0001 | 0.0009 | 0.0004 | 0.0016 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.0655 | 18.9473 | 14.0597 | 21.3944 | 25.8642 | 11.2149 | 26.2009 | 24.7193 | 19.8512 | 26.3153 |
Translational Efficiency | 0.7235 | 1.1272 | 1.2799 | 0.769 | 0.6978 | 1.746 | 0.7312 | 0.9999 | 0.9592 | 0.7773 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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