Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal stalk protein P1 alpha; involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 0 | 0 | – | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 2 | 0 | – | 7 | 10 | 8 | 3 | 6 | 0 | 0 | 2 | 0 | 5 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Cytoplasm | 124 | 66 | 72 | 170 | 153 | 135 | – | 212 | 130 | 141 | 115 | 122 | 118 | 177 | 173 | 35 | 63 | 23 | 129 | 86 | 60 | 52 | 118 | 137 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 2 | 3 | – | 12 | 6 | 23 | 10 | 14 | 0 | 2 | 0 | 1 | 7 | 1 | 0 | 0 | 1 | 0 | 1 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 1 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 |
Unique Cell Count | 125 | 67 | 76 | 172 | 159 | 138 | 229 | 145 | 169 | 128 | 146 | 118 | 182 | 174 | 35 | 66 | 23 | 135 | 92 | 72 | 56 | 125 | 146 | |
Labelled Cell Count | 126 | 67 | 76 | 172 | 161 | 138 | 235 | 151 | 172 | 130 | 146 | 118 | 183 | 175 | 38 | 76 | 24 | 135 | 92 | 72 | 56 | 125 | 146 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 294.8 | 311.4 | 188.4 | 272.8 | 237.9 | 191.8 | – | 153.5 | 133.7 | 128.4 | 111.2 | 109.1 | 306.0 | 300.3 | 287.5 | 318.8 | 302.9 | 292.7 | 294.2 | 339.1 | 336.0 |
Std Deviation (1e-4) | 59.5 | 103.5 | 79.6 | 72.6 | 76.3 | 59.2 | – | 42.5 | 35.1 | 33.2 | 31.6 | 32.5 | 67.1 | 73.4 | 56.7 | 126.2 | 109.0 | 106.1 | 82.2 | 85.3 | 85.0 |
Intensity Change (Log2) | – | – | – | 0.53 | 0.34 | 0.03 | – | -0.3 | -0.5 | -0.55 | -0.76 | -0.79 | 0.7 | 0.67 | 0.61 | 0.76 | 0.68 | 0.64 | 0.64 | 0.85 | 0.83 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.9 | 0.5 | 1.2 | – | -0.6 | -1.3 | -2.4 | -1.2 | -2.4 | 2.5 | 1.0 | 2.4 | 1.4 | 0.2 | 1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 221.8363 | 341.0973 | 298.6163 | 234.3487 | 224.3048 | 213.1921 | 198.6183 | 343.4382 | 281.3251 | 281.6279 | 266.4788 | 310.0589 | 263.0425 | 414.4092 | 359.8285 | 366.6771 | 308.5747 | 394.2698 |
Actin | 0.013 | 0.0015 | 0.0039 | 0.0691 | 0.0084 | 0.0239 | 0.0278 | 0.0007 | 0.0084 | 0.001 | 0.0104 | 0.0035 | 0.0398 | 0.0006 | 0.0051 | 0.0006 | 0.011 | 0.0111 |
Bud | 0.0044 | 0.0049 | 0.0083 | 0.0044 | 0.006 | 0.0173 | 0.0044 | 0.0029 | 0.0051 | 0.0059 | 0.0114 | 0.0052 | 0.0059 | 0.0032 | 0.0038 | 0.0038 | 0.0074 | 0.0051 |
Bud Neck | 0.0011 | 0.0011 | 0.0028 | 0.0012 | 0.0016 | 0.0026 | 0.0019 | 0.0006 | 0.0011 | 0.0018 | 0.0023 | 0.0015 | 0.0024 | 0.0006 | 0.0012 | 0.0016 | 0.001 | 0.0019 |
Bud Periphery | 0.0044 | 0.0025 | 0.0057 | 0.003 | 0.0082 | 0.0103 | 0.0056 | 0.001 | 0.003 | 0.0027 | 0.0103 | 0.0023 | 0.0046 | 0.0011 | 0.0015 | 0.0011 | 0.0026 | 0.0036 |
Bud Site | 0.0014 | 0.0031 | 0.0127 | 0.0068 | 0.002 | 0.0044 | 0.006 | 0.0019 | 0.003 | 0.0019 | 0.0013 | 0.0012 | 0.011 | 0.0022 | 0.0022 | 0.0004 | 0.001 | 0.0012 |
Cell Periphery | 0.0532 | 0.0247 | 0.0501 | 0.0304 | 0.007 | 0.007 | 0.0344 | 0.0188 | 0.0498 | 0.0562 | 0.0075 | 0.0313 | 0.0582 | 0.0234 | 0.0206 | 0.0092 | 0.0058 | 0.0038 |
Cytoplasm | 0.4262 | 0.7685 | 0.613 | 0.3524 | 0.3248 | 0.3932 | 0.4399 | 0.8478 | 0.5694 | 0.5163 | 0.456 | 0.5817 | 0.5004 | 0.8394 | 0.7859 | 0.6286 | 0.6098 | 0.6272 |
Cytoplasmic Foci | 0.0424 | 0.0025 | 0.0033 | 0.0078 | 0.0558 | 0.0789 | 0.0034 | 0.0013 | 0.0143 | 0.0027 | 0.0063 | 0.0075 | 0.004 | 0.0008 | 0.0029 | 0.0012 | 0.0118 | 0.0069 |
Eisosomes | 0.0036 | 0.0002 | 0.0004 | 0.0079 | 0.0007 | 0.0016 | 0.0248 | 0.0004 | 0.0049 | 0.0008 | 0.0001 | 0.0004 | 0.0093 | 0.0001 | 0.0075 | 0 | 0.0018 | 0.0006 |
Endoplasmic Reticulum | 0.0199 | 0.0024 | 0.0049 | 0.0058 | 0.0372 | 0.0063 | 0.024 | 0.0017 | 0.0029 | 0.0018 | 0.0081 | 0.0035 | 0.006 | 0.0022 | 0.0012 | 0.0003 | 0.004 | 0.0088 |
Endosome | 0.0094 | 0.002 | 0.0053 | 0.0142 | 0.0787 | 0.0739 | 0.014 | 0.0018 | 0.0058 | 0.0057 | 0.0321 | 0.0073 | 0.004 | 0.0009 | 0.0025 | 0.0021 | 0.0088 | 0.004 |
Golgi | 0.0037 | 0.0004 | 0.0017 | 0.0169 | 0.0442 | 0.0157 | 0.0029 | 0.0003 | 0.0027 | 0.0003 | 0.0058 | 0.0013 | 0.002 | 0.0001 | 0.0014 | 0.0001 | 0.0039 | 0.0011 |
Lipid Particles | 0.0104 | 0.0009 | 0.0028 | 0.0059 | 0.0114 | 0.015 | 0.004 | 0.0008 | 0.0059 | 0.0026 | 0.0014 | 0.0025 | 0.0046 | 0.0005 | 0.0026 | 0.0006 | 0.0057 | 0.0016 |
Mitochondria | 0.0802 | 0.0149 | 0.0184 | 0.0943 | 0.0272 | 0.0675 | 0.0676 | 0.0079 | 0.0601 | 0.0091 | 0.0801 | 0.1376 | 0.0793 | 0.0027 | 0.0188 | 0.0019 | 0.0868 | 0.1322 |
None | 0.0094 | 0.0018 | 0.0025 | 0.0088 | 0.0098 | 0.0455 | 0.0087 | 0.0012 | 0.0077 | 0.0022 | 0.0037 | 0.0042 | 0.0088 | 0.0014 | 0.0033 | 0.001 | 0.0128 | 0.0084 |
Nuclear Periphery | 0.0468 | 0.0061 | 0.0154 | 0.0205 | 0.0277 | 0.0301 | 0.0706 | 0.0063 | 0.0068 | 0.0127 | 0.0254 | 0.0322 | 0.0662 | 0.008 | 0.0089 | 0.0128 | 0.0123 | 0.0327 |
Nucleolus | 0.0314 | 0.0092 | 0.0189 | 0.0703 | 0.0093 | 0.0114 | 0.0142 | 0.0024 | 0.0146 | 0.0278 | 0.002 | 0.0208 | 0.0492 | 0.0021 | 0.0085 | 0.0055 | 0.0071 | 0.0096 |
Nucleus | 0.0502 | 0.0238 | 0.0303 | 0.0377 | 0.0261 | 0.0279 | 0.0386 | 0.0136 | 0.0171 | 0.03 | 0.015 | 0.0406 | 0.0459 | 0.0172 | 0.0203 | 0.057 | 0.0259 | 0.0293 |
Peroxisomes | 0.0099 | 0.0005 | 0.0009 | 0.0047 | 0.0061 | 0.0032 | 0.0013 | 0.0003 | 0.0015 | 0.0008 | 0.0007 | 0.0022 | 0.0016 | 0.0002 | 0.0005 | 0.0002 | 0.0011 | 0.0014 |
Punctate Nuclear | 0.0014 | 0.0001 | 0.0002 | 0.0004 | 0.0013 | 0.0024 | 0.002 | 0.0001 | 0.0006 | 0.0001 | 0.002 | 0.0005 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0011 | 0.0007 |
Vacuole | 0.124 | 0.0982 | 0.1449 | 0.1495 | 0.1737 | 0.0595 | 0.1273 | 0.0705 | 0.1547 | 0.2496 | 0.1722 | 0.0534 | 0.0575 | 0.077 | 0.0765 | 0.1694 | 0.0883 | 0.059 |
Vacuole Periphery | 0.0536 | 0.0307 | 0.0535 | 0.0879 | 0.1329 | 0.1024 | 0.0764 | 0.0176 | 0.0604 | 0.0679 | 0.1457 | 0.0591 | 0.0387 | 0.0162 | 0.0247 | 0.1024 | 0.09 | 0.0498 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3229.4345 | 3917.8493 | 5191.9408 | 2420.0662 | 3638.9245 | 4624.175 | 2846.7229 | 2401.0273 | 3508.7395 | 1984.2905 |
Translational Efficiency | 1.3409 | 1.2719 | 1.0769 | 3.5519 | 1.7141 | 0.8789 | 1.4058 | 2.7938 | 1.3226 | 1.9647 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
84 | 170 | 1698 | 1357 | 441 | 465 | 86 | 1403 | 525 | 635 | 1784 | 2760 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 12069.94 | 17313.42 | 23952.15 | 25254.52 | 11999.12 | 17210.86 | 25108.25 | 23945.10 | 12010.45 | 17238.32 | 24007.88 | 24588.90 |
Standard Deviation | 3086.92 | 4577.70 | 4581.77 | 5945.03 | 2955.40 | 4060.74 | 5938.22 | 6017.74 | 2976.94 | 4205.62 | 4662.81 | 6017.81 |
Intensity Change Log 2 | — | 0.520472 | 0.988737 | 1.065123 | — | 0.520391 | 1.065233 | 0.996802 | — | 0.520432 | 1.027379 | 1.031467 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000097 | 0.000096 | 0.000058 | 0.000145 | 0.000089 | 0.000104 | 0.000133 | 0.000271 | 0.000090 | 0.000102 | 0.000062 | 0.000209 |
Bud Neck | 0.000875 | 0.000875 | 0.000589 | 0.001040 | 0.000577 | 0.001174 | 0.001904 | 0.001360 | 0.000625 | 0.001094 | 0.000653 | 0.001203 |
Bud Site | 0.005741 | 0.004520 | 0.001813 | 0.005004 | 0.003369 | 0.002793 | 0.005881 | 0.007986 | 0.003749 | 0.003255 | 0.002009 | 0.006520 |
Cell Periphery | 0.067004 | 0.048872 | 0.008281 | 0.025207 | 0.034393 | 0.018462 | 0.052384 | 0.018478 | 0.039611 | 0.026603 | 0.010407 | 0.021787 |
Cytoplasm | 0.341220* | 0.446951* | 0.808517* | 0.613422* | 0.534142* | 0.644448* | 0.440378* | 0.647247* | 0.503274* | 0.591575* | 0.790770* | 0.630616* |
Cytoplasmic Foci | 0.022024 | 0.024381 | 0.007231 | 0.010696 | 0.012952 | 0.022403 | 0.011325 | 0.013520 | 0.014403 | 0.022933 | 0.007428 | 0.012131 |
Eisosomes | 0.000004 | 0.000006 | 0.000001 | 0.000003 | 0.000003 | 0.000003 | 0.000006 | 0.000004 | 0.000003 | 0.000004 | 0.000002 | 0.000004 |
Endoplasmic Reticulum | 0.001211 | 0.001994 | 0.000165 | 0.000321 | 0.001134 | 0.000711 | 0.000366 | 0.000271 | 0.001146 | 0.001054 | 0.000175 | 0.000295 |
Endosome | 0.001107 | 0.001178 | 0.000195 | 0.000487 | 0.000752 | 0.000429 | 0.005047 | 0.000688 | 0.000809 | 0.000629 | 0.000429 | 0.000589 |
Golgi | 0.127661* | 0.115205* | 0.024951 | 0.033844 | 0.083425 | 0.066810 | 0.028974 | 0.030536 | 0.090502 | 0.079767 | 0.025145 | 0.032163 |
Lipid Particles | 0.036010 | 0.027074 | 0.004137 | 0.012966 | 0.014804 | 0.008713 | 0.019407 | 0.012672 | 0.018197 | 0.013628 | 0.004873 | 0.012817 |
Mitochondria | 0.000286 | 0.000194 | 0.000188 | 0.000416 | 0.000198 | 0.000378 | 0.001612 | 0.000950 | 0.000212 | 0.000329 | 0.000257 | 0.000687 |
Mitotic Spindle | 0.003148 | 0.001751 | 0.000677 | 0.001406 | 0.001071 | 0.001352 | 0.000830 | 0.000825 | 0.001403 | 0.001459 | 0.000684 | 0.001111 |
None | 0.002691 | 0.002870 | 0.002281 | 0.002000 | 0.003515 | 0.002831 | 0.001289 | 0.005851 | 0.003383 | 0.002841 | 0.002234 | 0.003957 |
Nuclear Periphery | 0.002254 | 0.001506 | 0.000761 | 0.000757 | 0.001101 | 0.002491 | 0.001409 | 0.000599 | 0.001286 | 0.002227 | 0.000792 | 0.000677 |
Nuclear Periphery Foci | 0.012738 | 0.013356 | 0.006012 | 0.005688 | 0.011989 | 0.011994 | 0.005532 | 0.005746 | 0.012109 | 0.012358 | 0.005989 | 0.005717 |
Nucleolus | 0.000747 | 0.000107 | 0.000080 | 0.000224 | 0.000108 | 0.000336 | 0.000210 | 0.000190 | 0.000211 | 0.000275 | 0.000087 | 0.000207 |
Nucleus | 0.007966 | 0.012705 | 0.014546 | 0.020025 | 0.013144 | 0.021357 | 0.011859 | 0.026646 | 0.012316 | 0.019041 | 0.014416 | 0.023390 |
Peroxisomes | 0.000055 | 0.000038 | 0.000012 | 0.000053 | 0.000022 | 0.000028 | 0.000078 | 0.000062 | 0.000027 | 0.000030 | 0.000015 | 0.000057 |
Vacuole | 0.171102 | 0.154160 | 0.067186 | 0.179194 | 0.119660 | 0.090149 | 0.319300* | 0.168032 | 0.127891 | 0.107286 | 0.079339 | 0.173520 |
Vacuole Periphery | 0.196061* | 0.142163* | 0.052317 | 0.087104 | 0.163551* | 0.103036* | 0.092076 | 0.058068 | 0.168752* | 0.113511* | 0.054234 | 0.072344 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.12 | 1.16 | -2.11 | -3.60 | -6.28 | -0.10 | -0.73 | -4.08 | -3.32 | -2.56 | -0.07 | 1.48 | -4.50 | -3.56 | -6.68 |
Bud Neck | 0.18 | 0.65 | -0.76 | -1.85 | -3.86 | -2.11 | -4.04 | -5.62 | -0.97 | 1.49 | -2.07 | -1.04 | -5.66 | -1.14 | -5.05 |
Bud Site | 1.09 | 2.85 | 0.36 | -1.38 | -7.19 | 0.49 | -1.64 | -4.05 | -3.88 | -1.44 | 0.62 | 2.37 | -3.51 | -3.83 | -9.10 |
Cell Periphery | 1.25 | 4.63 | 3.30 | 4.09 | -11.06 | 4.57 | -1.35 | 5.09 | 0.01 | 3.11 | 3.36 | 8.74 | 5.36 | 2.20 | -9.96 |
Cytoplasm | -2.05 | -11.36 | -6.43 | -5.12 | 16.66 | -4.27 | 2.45 | -4.96 | 0.27 | -5.17 | -3.73 | -15.19 | -6.46 | -1.96 | 16.83 |
Cytoplasmic Foci | -0.31 | 2.24 | 1.69 | 3.09 | -2.68 | -3.42 | 0.07 | -0.85 | 2.97 | -0.64 | -3.37 | 3.60 | 0.77 | 4.43 | -4.54 |
Eisosomes | -1.16 | 2.56 | -0.10 | 1.21 | -5.24 | 0.69 | -1.53 | -2.10 | -2.73 | 0.67 | -0.28 | 3.14 | -1.74 | -1.17 | -6.44 |
Endoplasmic Reticulum | -0.58 | 2.90 | 2.49 | 1.30 | -4.99 | 0.69 | 2.38 | 2.77 | 0.86 | 0.40 | 0.17 | 3.59 | 3.17 | 1.49 | -4.90 |
Endosome | -0.26 | 4.56 | 2.79 | 2.50 | -2.98 | 2.04 | -0.95 | 0.37 | -1.22 | 0.97 | 1.16 | 1.86 | 1.46 | 0.42 | -0.86 |
Golgi | 0.69 | 7.33 | 6.78 | 8.14 | -4.10 | 2.34 | 8.23 | 9.58 | 8.03 | -0.58 | 1.58 | 12.33 | 11.31 | 11.01 | -3.96 |
Lipid Particles | 0.94 | 4.23 | 3.05 | 2.94 | -9.20 | 2.80 | -1.31 | 0.91 | -3.15 | 1.85 | 1.75 | 6.34 | 2.47 | 0.48 | -10.84 |
Mitochondria | 0.56 | 0.58 | -0.75 | -2.42 | -2.55 | -1.22 | -0.98 | -3.86 | -2.49 | 0.31 | -1.03 | -0.52 | -4.24 | -2.52 | -3.64 |
Mitotic Spindle | 0.81 | 1.43 | 0.88 | 0.27 | -1.67 | -1.02 | 0.77 | 0.34 | 1.29 | -0.45 | -0.32 | 1.95 | 0.28 | 0.68 | -1.85 |
None | -0.13 | 0.52 | 0.80 | 1.89 | 0.60 | 1.94 | 5.25 | -1.87 | -2.47 | -3.75 | 1.63 | 2.79 | -0.78 | -1.69 | -2.50 |
Nuclear Periphery | 0.73 | 1.49 | 1.58 | 3.71 | 0.87 | -1.28 | -0.66 | 4.26 | 1.69 | 1.72 | -1.18 | 2.10 | 3.27 | 1.91 | 2.11 |
Nuclear Periphery Foci | -0.38 | 3.08 | 3.50 | 5.06 | 1.41 | -0.23 | 4.42 | 5.39 | 5.19 | -0.34 | -0.44 | 5.38 | 6.36 | 6.91 | 1.30 |
Nucleolus | 1.02 | 1.05 | 0.82 | -1.82 | -2.19 | -2.00 | -3.32 | -4.99 | 1.26 | 0.68 | -0.56 | 1.17 | -0.01 | 0.78 | -3.60 |
Nucleus | -1.14 | -2.27 | -3.81 | -2.64 | -3.15 | -3.27 | -0.39 | -6.74 | -2.63 | -2.70 | -3.22 | -2.32 | -7.47 | -2.89 | -6.04 |
Peroxisomes | 0.90 | 3.08 | 0.20 | -0.76 | -3.15 | -0.39 | -2.29 | -4.12 | -1.91 | 0.53 | -0.24 | 2.88 | -3.44 | -1.88 | -5.35 |
Vacuole | 0.63 | 4.72 | -0.69 | -1.91 | -16.43 | 2.52 | -6.69 | -5.32 | -8.94 | 5.07 | 1.87 | 4.97 | -5.81 | -9.03 | -16.84 |
Vacuole Periphery | 2.06 | 6.36 | 4.94 | 4.22 | -6.96 | 4.72 | 4.32 | 10.12 | 5.76 | 1.85 | 4.81 | 11.73 | 10.27 | 6.17 | -4.43 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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