Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0.06 0 0 0.07 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0.05 0.05 0 0.06 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.93 0.96 0.91 0.75 0.83 0.72 0.78 0.62 0.68 0.63 0.55 0.79 – 0.95 0.91 0.9 0.92 0.51 0.81 0.84 0.85 0.82 0.77
Endoplasmic Reticulum – 0 0 0 0.05 0.06 0.13 0.12 0.09 0.07 0.05 0.05 0.05 – 0 0 0 0 0 0 0 0 0 0.06
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0.21 – 0 0 0 0 0.2 0 0 0 0 0
Golgi – 0 0 0 0 0 0.06 0 0 0 0 0 0 – 0 0 0.07 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0.16 0.06 0.06 0 0.29 0.22 0.34 0.4 0 – 0 0.05 0 0 0 0.06 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0.07 0 0.06 0.06 0.05 0.05 0 0.09 0 – 0 0 0.07 0.06 0.14 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 1 2 6 9 4 3 12 2 4 0 – 0 0 0 0 0 2 1 0 0 0
Bud – 0 0 0 2 0 0 0 2 2 1 0 0 – 0 0 0 0 0 1 2 2 3 0
Bud Neck – 0 0 0 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 – – – – – –
Cell Periphery – 3 4 3 5 7 7 13 2 9 1 1 0 – 1 8 8 7 0 0 1 0 0 0
Cytoplasm – 163 164 77 77 124 114 171 87 115 59 52 15 – 62 225 172 163 4 162 153 52 78 57
Endoplasmic Reticulum – 4 4 0 5 9 21 27 13 12 5 5 1 – 0 6 7 3 0 3 6 1 2 4
Endosome – 0 0 2 0 0 0 0 1 0 1 0 4 – 0 1 0 0 1 1 3 0 1 0
Golgi – 0 1 1 1 2 10 7 5 4 1 4 0 – 0 11 14 5 0 0 1 0 0 1
Mitochondria – 7 0 2 16 9 9 4 41 38 32 38 0 – 1 12 5 2 0 11 3 0 1 1
Nucleus – 0 1 2 2 0 0 0 0 0 1 1 0 – 0 0 1 0 0 2 0 0 0 0
Nuclear Periphery – 0 0 0 0 1 0 0 0 0 0 1 0 – 1 0 0 0 0 2 2 0 0 2
Nucleolus – 0 0 0 2 0 0 0 0 0 1 0 0 – 0 0 0 0 0 1 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane – 6 2 1 7 1 9 14 7 8 4 8 0 – 1 10 14 10 1 5 2 0 3 1
Unique Cell Count 175 171 85 102 149 158 219 141 170 93 94 19 65 248 192 178 8 200 182 62 96 74
Labelled Cell Count 183 176 89 120 159 179 240 161 200 108 114 20 66 273 221 190 8 200 182 62 96 74


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 11.6 15.9 17.5 12.5 16.9 15.5 15.1 12.3 12.9 11.7 12.3 12.7 – 14.9 22.5 29.6 27.9 14.6 14.1 14.1
Std Deviation (1e-4) – 4.1 5.4 6.8 6.1 6.3 5.4 5.1 5.3 3.9 4.5 4.8 7.5 – 6.3 7.9 11.9 11.5 5.5 7.3 5.8
Intensity Change (Log2) – – – 0.14 -0.35 0.09 -0.04 -0.07 -0.37 -0.29 -0.44 -0.37 -0.32 – -0.09 0.51 0.9 0.81 -0.12 -0.17 -0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 3.5 0 0 0 – 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Cell Periphery 0 0 1.2 1.1 1.7 0 1.4 0 0 0 – 0 0 1.0 0.9
Cytoplasm -1.7 -5.1 -3.8 -5.9 -5.0 -7.6 -6.8 -7.0 -8.1 -3.0 – -0.2 -2.0 -2.3 -1.7
Endoplasmic Reticulum 0 0 1.7 3.7 3.6 2.7 2.1 0 0 0 – 0 0 0.7 0
Endosome 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Golgi 0 0 0 2.9 0 0 0 0 0 0 – 0 0 3.2 0
Mitochondria 0 5.3 0 0 0 7.6 6.6 8.2 9.0 0 – 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Vacuole 0 0 0 2.3 2.6 0 0 0 0 0 – 0 0 2.8 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0035 0.0011 0.008 0.0007 0.0016 0.0069 – – – – – – 0.0332 0.0055 0.0016 0.0014 0.0012 0.0115
Bud 0.0059 0.002 0.0162 0.0005 0.0065 0.0058 – – – – – – 0.0043 0.0005 0.0017 0.0019 0.0091 0.004
Bud Neck 0.0034 0.0007 0.0014 0.0005 0.0007 0.0025 – – – – – – 0.0026 0.0005 0.0016 0.0007 0.001 0.0025
Bud Periphery 0.0064 0.0011 0.0266 0.0001 0.0098 0.0089 – – – – – – 0.0031 0.0002 0.0031 0.0004 0.0278 0.0044
Bud Site 0.0029 0.002 0.0047 0.0005 0.0006 0.0026 – – – – – – 0.0192 0.0011 0.0063 0.0003 0.0009 0.0006
Cell Periphery 0.008 0.0058 0.0048 0.0003 0.0023 0.0026 – – – – – – 0.0085 0.0023 0.0061 0.0004 0.0031 0.0007
Cytoplasm 0.5427 0.9375 0.6192 0.1893 0.4601 0.6536 – – – – – – 0.6545 0.9457 0.8917 0.8445 0.8136 0.8185
Cytoplasmic Foci 0.0164 0.0027 0.0229 0.088 0.0217 0.0158 – – – – – – 0.0193 0.0024 0.0027 0.0065 0.0029 0.0345
Eisosomes 0.0007 0 0.0019 0 0.0001 0 – – – – – – 0.0002 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0571 0.0065 0.0054 0.0025 0.0041 0.0404 – – – – – – 0.1092 0.0068 0.0089 0.0027 0.0438 0.0293
Endosome 0.0365 0.004 0.0599 0.3238 0.0492 0.088 – – – – – – 0.0116 0.0023 0.0081 0.0233 0.015 0.0435
Golgi 0.0069 0.0012 0.0194 0.0716 0.0105 0.0207 – – – – – – 0.0027 0.0006 0.0007 0.0012 0.0004 0.0079
Lipid Particles 0.0025 0.0022 0.0156 0.0012 0.0043 0.0011 – – – – – – 0.0027 0.0003 0.0004 0.0001 0.0003 0.0021
Mitochondria 0.0153 0.0013 0.0457 0.0064 0.0148 0.0265 – – – – – – 0.0012 0.0002 0.0021 0.0013 0.0075 0.0055
None 0.1236 0.0002 0.0339 0.0004 0.2535 0.0009 – – – – – – 0.0199 0.0001 0.0004 0.0002 0.0005 0.0086
Nuclear Periphery 0.0164 0.0013 0.0018 0.0067 0.0029 0.008 – – – – – – 0.0085 0.0014 0.0028 0.0037 0.0076 0.0029
Nucleolus 0.004 0.0001 0.0021 0.0001 0.0013 0.0126 – – – – – – 0.0007 0 0.0003 0 0.0006 0.0002
Nucleus 0.0182 0.0014 0.0024 0.0021 0.0019 0.0182 – – – – – – 0.0043 0.001 0.0025 0.002 0.0045 0.0018
Peroxisomes 0.0005 0.0001 0.0052 0.0022 0.0022 0.0003 – – – – – – 0.0009 0.0025 0.0001 0.0002 0.0001 0.0006
Punctate Nuclear 0.0299 0 0.0006 0.0003 0.0003 0.0003 – – – – – – 0.0053 0 0.0001 0 0.0001 0.0008
Vacuole 0.0723 0.0258 0.0809 0.235 0.0957 0.0529 – – – – – – 0.0806 0.0254 0.0449 0.0877 0.0471 0.0146
Vacuole Periphery 0.0268 0.003 0.0212 0.0678 0.056 0.0312 – – – – – – 0.0076 0.0013 0.014 0.0217 0.0129 0.0057

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1674.263 1721.5917 1953.5174 1846.3177 2005.1751 1324.884 1546.7755 1442.859 1470.1273 1531.3353
Translational Efficiency 1.3957 1.3988 1.3173 1.1634 1.3978 1.9016 1.4419 1.462 1.5177 1.6715

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
226 368 2636 1264 327 2314 755 1048 553 2682 3391 2312

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1125.81 1734.71 1916.04 2064.58 1600.20 1007.02 2487.03 2125.10 1406.33 1106.87 2043.17 2092.01
Standard Deviation 294.42 465.64 792.35 433.03 269.98 385.46 687.58 446.31 364.57 469.71 806.05 440.13
Intensity Change Log 2 — 0.623731 0.767164 0.874885 — -0.668160 0.636172 0.409279 — 0.008296 0.691719 0.620049

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000465 0.000260 0.000207 0.000238 0.000320 0.000404 0.000468 0.000277 0.000379 0.000384 0.000265 0.000256
Bud Neck 0.002642 0.001311 0.002243 0.001755 0.000535 0.011461 0.002440 0.001169 0.001396 0.010068 0.002287 0.001489
Bud Site 0.060742 0.032269 0.027810 0.014943 0.028253 0.048816 0.020601 0.018149 0.041531 0.046545 0.026205 0.016396
Cell Periphery 0.002594 0.003439 0.003224 0.003418 0.008925 0.000628 0.015135 0.008011 0.006338 0.001014 0.005876 0.005500
Cytoplasm 0.557501* 0.644502* 0.794524* 0.605098* 0.533290* 0.600014* 0.572485* 0.709968* 0.543185* 0.606118* 0.745087* 0.652634*
Cytoplasmic Foci 0.200918* 0.091968 0.013272 0.031549 0.129972 0.144472 0.021221 0.030893 0.158966 0.137268 0.015042 0.031252
Eisosomes 0.000036 0.000006 0.000003 0.000004 0.000004 0.000055 0.000005 0.000003 0.000017 0.000048 0.000003 0.000003
Endoplasmic Reticulum 0.008369 0.002952 0.001245 0.001484 0.010466 0.002163 0.001305 0.001335 0.009609 0.002271 0.001258 0.001417
Endosome 0.012862 0.008185 0.001759 0.007977 0.009295 0.015568 0.003970 0.005772 0.010752 0.014555 0.002251 0.006978
Golgi 0.045712 0.106825 0.024904 0.066769 0.127176* 0.008653 0.091049 0.083121 0.093884 0.022124 0.039631 0.074181
Lipid Particles 0.002844 0.003487 0.002655 0.008700 0.005048 0.006730 0.025740 0.013119 0.004147 0.006285 0.007795 0.010703
Mitochondria 0.001529 0.003046 0.001134 0.001437 0.000363 0.003842 0.000583 0.000973 0.000839 0.003733 0.001011 0.001227
Mitotic Spindle 0.001616 0.003258 0.006360 0.007314 0.002720 0.007100 0.006175 0.004734 0.002269 0.006572 0.006319 0.006144
None 0.000916 0.001216 0.002416 0.001121 0.000303 0.004471 0.000742 0.000504 0.000553 0.004024 0.002043 0.000841
Nuclear Periphery 0.000065 0.000123 0.000247 0.000586 0.000075 0.000490 0.000529 0.000204 0.000071 0.000439 0.000310 0.000413
Nuclear Periphery Foci 0.001499 0.004788 0.004133 0.009689 0.004012 0.001145 0.014189 0.008022 0.002985 0.001645 0.006372 0.008934
Nucleolus 0.000210 0.000215 0.000188 0.000351 0.000032 0.001571 0.000096 0.000069 0.000105 0.001385 0.000168 0.000223
Nucleus 0.015562 0.003961 0.019102 0.006512 0.003469 0.067726 0.007870 0.004284 0.008411 0.058976 0.016602 0.005502
Peroxisomes 0.001151 0.000477 0.000082 0.000298 0.000128 0.004349 0.000917 0.000145 0.000546 0.003818 0.000268 0.000229
Vacuole 0.024152 0.020796 0.055589 0.157507 0.021017 0.055635 0.151938 0.067890 0.022298 0.050855 0.077041 0.116885
Vacuole Periphery 0.058614 0.066917 0.038904 0.073252 0.114597* 0.014709 0.062541 0.041357 0.091718 0.021872 0.044167 0.058794

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.33 2.57 2.46 0.02 -0.14 -0.97 -1.25 0.74 1.70 1.60 -0.04 1.26 2.36 1.86 1.14
Bud Neck 1.27 -1.08 0.54 -1.95 4.65 -15.86 -6.53 -4.51 14.34 4.03 -12.60 -5.01 -1.13 13.69 6.74
Bud Site 4.08 4.96 8.00 4.05 8.15 -6.05 1.88 3.24 10.88 1.03 -1.42 3.79 8.36 13.37 7.33
Cell Periphery -1.41 -1.04 -1.31 0.26 -0.53 7.46 -3.28 0.96 -9.07 4.77 7.65 1.10 1.36 -10.20 0.49
Cytoplasm -3.43 -10.56 -2.11 2.19 15.42 -3.17 -1.30 -8.69 -10.29 -9.39 -3.97 -12.70 -7.27 -5.75 8.40
Cytoplasmic Foci 7.54 14.18 12.76 9.36 -10.31 -2.12 13.83 12.50 27.79 -4.23 2.33 19.79 17.46 29.94 -11.78
Eisosomes 3.76 3.92 4.01 1.58 1.68 -18.33 -2.66 1.35 18.69 3.80 -7.41 3.63 4.03 18.54 3.79
Endoplasmic Reticulum 2.92 3.98 3.89 3.05 -0.88 3.79 4.14 4.18 2.86 0.34 4.91 5.59 5.53 3.82 -0.48
Endosome 2.78 8.48 3.44 0.29 -8.21 -6.55 6.38 3.81 11.73 -2.89 -4.61 11.82 4.65 10.47 -9.57
Golgi -6.63 4.79 -3.21 5.04 -11.94 14.82 4.66 5.31 -16.62 0.87 12.98 10.32 3.94 -16.50 -11.49
Lipid Particles -0.86 0.38 -6.78 -6.26 -8.49 -1.99 -9.73 -6.03 -4.65 5.41 -3.16 -5.11 -8.32 -4.95 -3.84
Mitochondria -0.58 0.37 0.17 0.62 -0.06 -6.43 -0.75 -0.89 3.46 -0.48 -4.59 -1.21 -0.57 3.25 -0.03
Mitotic Spindle -2.64 -7.81 -4.60 -2.90 1.93 -4.43 -3.16 -2.39 1.06 1.99 -5.28 -7.90 -4.92 -0.17 3.17
None -0.84 -5.30 -0.43 0.26 3.30 -6.79 -6.12 -3.94 6.45 3.68 -6.44 -8.20 -1.26 5.59 4.91
Nuclear Periphery -1.98 -9.24 -1.61 -1.40 -0.49 -12.75 -4.67 -3.50 5.02 3.27 -13.02 -9.86 -2.01 0.22 0.32
Nuclear Periphery Foci -4.53 -7.32 -11.12 -4.94 -7.20 4.16 -7.52 -4.32 -10.02 4.68 2.90 -6.61 -9.43 -14.04 -3.85
Nucleolus -0.08 -1.68 -0.84 -0.79 0.67 -7.98 -4.61 -1.55 6.19 -0.74 -7.68 -2.51 -1.87 6.10 0.73
Nucleus 3.27 -3.52 2.14 -2.90 12.09 -16.80 -2.87 -0.86 23.93 3.44 -16.22 -6.69 0.51 22.77 13.45
Peroxisomes 1.64 2.65 2.12 0.99 -1.57 -7.92 -1.02 -1.15 7.87 0.98 -6.70 1.25 1.84 7.72 0.25
Vacuole 0.88 -8.92 -19.69 -22.19 -15.49 -11.03 -17.26 -10.55 -3.06 10.42 -10.56 -18.74 -22.43 -16.27 -7.73
Vacuole Periphery -0.90 3.10 -1.39 -0.32 -8.49 10.88 5.77 7.90 -8.52 4.09 10.95 7.94 5.41 -12.54 -5.40
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Rps16b

Rps16b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps16b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available