Standard name
Human Ortholog
Description FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 – 0 0 0 0.07 0.11 0.15 0.14 0.11 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.08 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.08 0.05 0 0 0.05 0.05 0.06 0.07
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.09 0.15 0.15 0.39 0.42 0.41 – 0.51 0.52 0.47 0 0 0 0.08 0.08 0.11 0 0 0 0.05 0.09 0.1
Nucleus 0.13 0.15 0.07 0 0.06 0.06 0.09 0.06 – 0.08 0.09 0.08 0.09 0.05 0.05 0.15 0.11 0.14 0.05 0.06 0 0 0 0
Nuclear Periphery 0.68 0.67 0.78 0.78 0.7 0.62 0.56 0.51 – 0.52 0.51 0.51 0.77 0.75 0.64 0.54 0.55 0.57 0.76 0.7 0.83 0.75 0.76 0.72
Nucleolus 0.15 0.08 0.15 0.2 0.2 0.27 0.28 0.33 – 0.32 0.32 0.34 0.13 0.08 0.09 0.13 0.11 0.11 0.06 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.29 0.27 0.14 0.08 0.09 0 0 0.06 – 0 0 0.05 0.21 0.21 0.23 0.3 0.34 0.27 0.06 0.12 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 1 0 1 0 0 0 0 0 2 1 3 3 4
Bud 0 0 0 1 1 2 1 4 – 2 3 2 1 1 0 0 0 0 3 6 3 2 5 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 2 7 3 – 9 8 3 0 5 4 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 – 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 13 6 10 14 9 8 11 – 6 1 7 22 74 72 26 26 15 0 2 1 0 1 0
Endoplasmic Reticulum 0 3 0 0 2 0 2 19 – 1 2 1 0 2 1 4 6 3 0 0 0 1 2 2
Endosome 2 0 34 2 11 7 4 9 – 1 3 7 7 23 15 11 19 6 7 15 23 23 44 61
Golgi 0 0 2 0 0 0 0 0 – 0 0 0 0 1 0 0 1 0 0 1 3 4 5 6
Mitochondria 1 26 38 39 45 168 230 230 – 255 223 191 2 1 2 15 19 13 6 7 15 23 67 79
Nucleus 37 61 30 8 19 26 51 32 – 41 37 31 26 35 27 28 26 16 19 31 6 4 3 4
Nuclear Periphery 190 279 329 204 218 266 308 286 – 258 221 206 236 517 317 99 132 65 270 364 416 334 564 597
Nucleolus 41 32 64 52 61 118 156 183 – 158 137 138 39 58 45 24 27 13 20 22 9 9 8 14
Peroxisomes 0 0 0 0 0 0 0 0 – 0 1 1 0 2 4 0 0 0 0 0 0 6 8 12
SpindlePole 1 0 4 1 3 2 2 1 – 3 2 1 1 18 27 0 1 0 3 1 4 3 4 8
Vac/Vac Membrane 81 111 60 20 27 19 21 35 – 12 17 21 65 143 111 55 82 31 21 61 15 25 20 27
Unique Cell Count 278 418 422 263 310 431 554 556 498 432 403 305 686 492 184 240 115 355 522 505 444 745 828
Labelled Cell Count 355 526 567 337 401 619 790 813 747 655 611 399 881 625 262 339 162 355 522 505 444 745 828


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 9.9 9.0 8.5 7.7 6.6 6.3 6.6 – 6.0 6.2 6.6 10.3 10.4 10.5 7.3 8.7 9.2 11.4 11.8 12.1
Std Deviation (1e-4) 2.3 3.0 2.3 2.5 2.2 1.8 1.8 1.9 – 2.1 2.0 2.3 2.2 2.7 2.8 1.7 2.2 2.3 2.2 2.0 2.5
Intensity Change (Log2) – – – -0.08 -0.22 -0.43 -0.5 -0.44 – -0.57 -0.54 -0.45 0.21 0.22 0.23 -0.29 -0.03 0.04 0.35 0.4 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 2.5 0.7 0 0.7 – -0.3 0 0.4 4.0 5.8 7.1 6.4 5.4 0
Endoplasmic Reticulum 0 0 0 0 3.8 – 0 0 0 0 0 0 0 0 0
Endosome -4.2 -2.5 -4.4 -5.9 -4.9 – -6.2 -5.3 -4.2 -3.3 -3.4 -3.4 -0.9 -0.1 -1.0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 2.3 2.3 10.2 11.3 11.2 – 13.7 13.5 12.3 -4.9 -7.8 -6.3 -0.3 -0.5 0.7
Nucleus -2.3 -0.5 -0.6 1.2 -0.9 – 0.6 0.8 0.3 0.7 -1.4 -1.0 3.1 1.7 2.3
Nuclear Periphery -0.1 -2.4 -5.2 -7.3 -8.5 – -8.2 -8.2 -8.1 -0.2 -1.0 -4.5 -6.0 -6.2 -4.6
Nucleolus 1.6 1.6 4.4 4.8 6.3 – 5.8 5.7 6.4 -0.9 -3.5 -2.8 -0.7 -1.4 -1.0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 1.9 3.8 0 0 0
Vacuole -2.6 -2.3 -4.9 -5.9 -4.1 – -6.6 -5.2 -4.3 2.5 2.8 3.2 4.5 6.0 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.1821 7.311 7.91 7.4 8.1585 7.9786 10.4364 9.5715 9.9874 10.5565 9.1311 9.8314 11.0107 10.1428 10.0018 10.1866 10.3859 9.953
Actin 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0.0009 0.0001 0.0001 0.0002 0.0002 0.0001 0.0008 0.0002 0.0001 0.0003 0.0001 0.0001
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0.0014 0 0 0 0 0
Bud Neck 0 0.0001 0 0 0.0001 0.0001 0.0004 0 0 0 0 0 0.0001 0 0 0 0.0001 0
Bud Periphery 0 0 0 0 0.0001 0.0001 0.0001 0 0 0 0 0 0.0003 0 0 0 0 0
Bud Site 0 0.0003 0 0 0.0001 0 0.0022 0 0 0 0 0 0.0002 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.0002 0 0 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.0001 0.006 0.0001 0.0002 0.0001 0.0002 0.0003 0 0 0.0001 0 0 0.0021 0.0001 0 0 0 0
Cytoplasmic Foci 0 0.0002 0 0 0.0118 0 0.0009 0 0 0 0.0002 0 0.0034 0 0 0 0.0001 0
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0002 0.0007 0.0002 0.0002 0.0003 0.0001 0.0004 0.0003 0.0002 0.0003 0.0003 0.0003 0.0011 0.0002 0.0003 0.0005 0.0001 0.0001
Endosome 0.0002 0.002 0.0002 0.0001 0.0008 0.0002 0.0017 0.0001 0.0001 0.0002 0.0005 0.0004 0.0027 0.0001 0.0001 0.0002 0.0002 0.0001
Golgi 0 0 0 0 0.0003 0 0.0003 0 0 0 0.0002 0.0001 0.0004 0 0 0.0001 0.0002 0
Lipid Particles 0.0002 0.0002 0.0002 0.0001 0.0235 0.0004 0.0035 0.0004 0.0003 0.0002 0.0031 0.0005 0.0035 0.0002 0.0006 0.0005 0.0027 0.0004
Mitochondria 0.0003 0.0002 0.0002 0.0006 0.0009 0.0004 0.0011 0.0001 0.0002 0.0003 0.0017 0.0009 0.0011 0.0002 0.0003 0.0004 0.0009 0.0002
None 0.0001 0.0002 0 0.0001 0.0001 0.0001 0.0005 0 0 0.0001 0.0002 0 0.0028 0 0.0001 0.0001 0.0001 0
Nuclear Periphery 0.8929 0.6963 0.8384 0.916 0.8383 0.8985 0.9426 0.9178 0.9393 0.9005 0.9219 0.9741 0.926 0.924 0.9239 0.9654 0.8893 0.9591
Nucleolus 0.0024 0.0142 0.0063 0.0012 0.0292 0.008 0.001 0.0021 0.005 0.0006 0.0091 0.0008 0.0031 0.0054 0.0035 0.0005 0.0144 0.0015
Nucleus 0.0909 0.2567 0.1384 0.068 0.0732 0.082 0.0293 0.062 0.0407 0.0838 0.0417 0.0146 0.03 0.0549 0.0524 0.0125 0.0779 0.0299
Peroxisomes 0 0 0 0 0.0001 0 0.0008 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Punctate Nuclear 0.0019 0.0049 0.0019 0.0015 0.0044 0.0019 0.0029 0.0054 0.0008 0.0009 0.0033 0.0006 0.0055 0.0018 0.0013 0.0015 0.0035 0.001
Vacuole 0.0003 0.0082 0.0007 0.0001 0.0009 0.0004 0.0004 0.0002 0.0002 0.0001 0.0004 0.0002 0.0019 0.0002 0.0002 0.0002 0.0002 0.0001
Vacuole Periphery 0.0105 0.0097 0.0132 0.0117 0.0156 0.0074 0.0104 0.0113 0.0131 0.0128 0.0171 0.0075 0.0133 0.0126 0.0171 0.0179 0.0099 0.0075

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.2489 20.5107 17.5258 26.2976 20.7335 14.5927 21.5238 26.9335 20.1477 25.5794
Translational Efficiency 1.1221 1.1032 1.2706 0.7213 1.1893 1.7179 1.0583 0.8209 1.1492 0.9996

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
130 822 105 1062 1616 1116 827 927 1746 1938 932 1989

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1005.61 1056.95 1140.31 1130.60 780.85 986.67 1169.37 1089.41 797.58 1016.48 1166.10 1111.40
Standard Deviation 161.01 193.02 248.71 194.20 142.92 169.42 192.15 181.55 155.94 183.13 199.54 189.53
Intensity Change Log 2 — 0.071836 0.181355 0.169018 — 0.337522 0.582614 0.480430 — 0.194023 0.370589 0.313463

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000935 0.000304 0.001367 0.000278 0.000643 0.000574 0.000383 0.000277 0.000665 0.000459 0.000494 0.000278
Bud Neck 0.003181 0.000699 0.002961 0.002397 0.001011 0.000563 0.000835 0.001670 0.001172 0.000621 0.001075 0.002058
Bud Site 0.003691 0.000095 0.001492 0.000587 0.000953 0.000296 0.000101 0.000465 0.001157 0.000211 0.000257 0.000530
Cell Periphery 0.000087 0.000028 0.000201 0.000035 0.000157 0.000060 0.000026 0.000032 0.000152 0.000046 0.000045 0.000034
Cytoplasm 0.008701 0.000490 0.049172 0.001208 0.003860 0.001879 0.002872 0.000837 0.004220 0.001290 0.008088 0.001035
Cytoplasmic Foci 0.006917 0.000299 0.013351 0.001079 0.001855 0.001117 0.000261 0.001810 0.002232 0.000770 0.001736 0.001420
Eisosomes 0.000020 0.000004 0.000093 0.000005 0.000016 0.000012 0.000004 0.000004 0.000016 0.000008 0.000014 0.000005
Endoplasmic Reticulum 0.013455 0.004723 0.009012 0.001855 0.009254 0.007206 0.001851 0.003299 0.009567 0.006153 0.002658 0.002528
Endosome 0.004987 0.000998 0.003279 0.001992 0.003131 0.001673 0.000347 0.002895 0.003269 0.001387 0.000677 0.002413
Golgi 0.006954 0.000420 0.000333 0.000950 0.000908 0.000937 0.000132 0.002382 0.001358 0.000717 0.000155 0.001617
Lipid Particles 0.003721 0.000640 0.003410 0.001281 0.003417 0.001768 0.000835 0.001358 0.003440 0.001290 0.001125 0.001317
Mitochondria 0.015178 0.002180 0.002918 0.002973 0.007340 0.005468 0.001859 0.002428 0.007924 0.004073 0.001978 0.002719
Mitotic Spindle 0.069368 0.004429 0.014881 0.045679 0.001709 0.006818 0.012274 0.046841 0.006747 0.005805 0.012568 0.046221
None 0.001282 0.001191 0.003996 0.000344 0.006257 0.007209 0.000379 0.000491 0.005887 0.004657 0.000786 0.000413
Nuclear Periphery 0.349363* 0.496825* 0.286516* 0.516634* 0.425784* 0.424031* 0.625728* 0.468363* 0.420094* 0.454907* 0.587512* 0.494137*
Nuclear Periphery Foci 0.342947* 0.409851* 0.179584 0.377930* 0.413932* 0.349397* 0.263688* 0.414056* 0.408647* 0.375038* 0.254213* 0.394767*
Nucleolus 0.044912 0.027743 0.083735 0.011644 0.051109 0.062708 0.036238 0.011852 0.050647 0.047878 0.041589 0.011741
Nucleus 0.094945 0.038519 0.316078* 0.017635 0.055174 0.121630 0.048580 0.031270 0.058135 0.086379 0.078717 0.023990
Peroxisomes 0.001293 0.000247 0.002051 0.000241 0.000964 0.000830 0.000341 0.000762 0.000989 0.000583 0.000534 0.000484
Vacuole 0.014988 0.004541 0.024008 0.005714 0.005558 0.002363 0.000979 0.003673 0.006260 0.003287 0.003574 0.004763
Vacuole Periphery 0.013076 0.005774 0.001563 0.009537 0.006965 0.003460 0.002286 0.005235 0.007420 0.004442 0.002205 0.007532

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.39 -1.29 2.39 0.10 4.30 0.84 3.47 5.68 5.14 1.71 3.43 1.84 6.13 3.94 3.66
Bud Neck 2.76 -0.26 0.62 -4.41 0.82 1.77 0.22 -2.42 -3.92 -2.88 2.34 -0.32 -3.35 -5.94 -3.37
Bud Site 2.34 1.07 1.95 -2.36 1.73 1.55 2.56 0.88 -0.86 -2.29 2.79 2.47 1.42 -2.06 -1.54
Cell Periphery 2.72 -3.35 2.30 -1.58 5.64 4.19 5.84 5.79 3.08 -0.62 5.18 4.85 5.90 2.01 1.98
Cytoplasm 2.08 -3.37 1.64 -2.40 4.14 1.62 0.06 3.31 2.34 2.16 3.00 -2.44 2.73 -0.33 4.01
Cytoplasmic Foci 1.80 -1.70 1.55 -2.03 3.94 1.09 3.53 0.13 -0.82 -2.68 2.56 0.47 1.31 -1.49 0.87
Eisosomes 2.92 -4.36 2.62 -0.92 5.69 1.61 6.17 5.86 5.41 -0.34 4.04 0.31 5.79 3.10 4.29
Endoplasmic Reticulum 1.32 0.55 1.77 4.62 5.92 2.17 11.43 8.47 5.05 -3.95 3.84 8.71 9.07 7.08 0.62
Endosome 3.25 0.75 2.30 -2.18 2.19 1.97 4.38 0.22 -1.38 -3.19 2.96 4.17 1.10 -2.14 -3.73
Golgi 1.35 1.37 1.23 -1.40 -1.93 -0.03 3.72 -1.52 -1.38 -2.42 1.29 3.05 -0.47 -1.67 -3.18
Lipid Particles 2.40 -0.30 1.78 -1.22 3.10 2.93 3.92 3.87 0.81 -0.89 4.71 3.65 4.19 -0.07 -0.20
Mitochondria 1.75 1.59 1.60 -1.12 0.01 1.61 6.08 5.69 2.92 -1.06 3.74 5.96 5.09 1.67 -1.28
Mitotic Spindle 4.34 3.45 1.69 -8.82 -4.47 -3.33 -4.97 -8.74 -7.53 -6.00 0.79 -2.64 -10.75 -11.45 -8.20
None 0.25 -1.69 1.70 1.03 2.54 -0.52 5.86 5.76 3.79 -0.42 0.81 5.35 5.94 3.93 2.04
Nuclear Periphery -5.58 1.59 -6.07 -1.10 -7.56 0.32 -16.89 -3.21 -3.21 12.12 -3.74 -13.68 -7.50 -3.87 8.07
Nuclear Periphery Foci -2.63 5.12 -1.35 3.31 -9.81 6.30 16.39 0.37 -5.21 -13.70 3.87 17.47 2.06 -1.99 -16.62
Nucleolus 2.15 -1.71 3.62 4.77 5.16 -2.50 3.19 10.93 11.55 6.17 0.69 2.14 12.23 12.36 8.04
Nucleus 3.36 -6.19 4.35 3.63 9.65 -8.31 0.46 3.86 10.62 2.83 -4.81 -3.43 7.30 11.52 8.34
Peroxisomes 1.59 -1.12 1.59 0.10 6.78 0.43 3.92 0.16 0.03 -0.82 2.22 2.85 1.36 0.26 0.01
Vacuole 2.12 -1.76 1.62 -1.58 3.22 3.10 4.28 1.09 -2.10 -3.41 2.79 1.07 0.39 -2.72 -0.79
Vacuole Periphery 1.12 1.83 0.51 -2.38 -6.25 3.51 5.68 1.58 -1.74 -3.43 2.96 5.88 -0.11 -3.28 -6.53
Standard name
Human Ortholog
Description FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication
Localization
Cell Percentages nuclear periphery (95%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Asm4

Asm4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Asm4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available