Standard name
Human Ortholog
Description Protein involved in regulation of mitotic exit; dephosphorylation target of Cdc14p in anaphase, which promotes timely rDNA segregation and allows mitotic progression; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.83 0.92 0.88 0.88 0.73 0.62 0.64 0.42 0.48 0.35 0.42 0.98 0.97 0.95 – 0.33 0.21 0.61 0.63 0.53 0.7 0.75 0.63
Endoplasmic Reticulum 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.07 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.26 0.27 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.22 0.22 0.07 0.21 0.11 0.45 0.5 0.43 0.68 0.73 0.78 0.74 0 0 0 – 0.15 0.09 0.15 0.1 0.21 0.05 0.06 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.06 0 0 0.06 0 0 0 – 0.4 0.52 0.06 0.12 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 2 1 2 5 6 4
Bud 2 0 0 1 0 1 1 2 3 3 2 5 0 0 0 – 2 1 2 0 1 1 5 8
Bud Neck 0 0 0 0 1 0 0 4 0 0 0 0 0 0 1 – 0 0 2 0 2 0 0 1
Bud Site 0 0 0 0 0 0 1 1 4 2 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 1 1 0 0 0 2 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 2
Cytoplasm 185 68 125 144 170 213 228 263 106 147 85 120 162 188 170 – 87 34 151 63 84 68 170 135
Endoplasmic Reticulum 1 3 8 0 2 4 0 0 1 1 2 0 0 0 2 – 10 7 10 2 11 1 4 6
Endosome 1 0 0 1 3 0 1 6 0 0 0 0 0 1 0 – 67 44 6 2 4 3 2 3
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 1
Mitochondria 46 18 10 35 21 132 183 176 172 225 187 213 3 3 3 – 40 15 37 9 34 5 14 18
Nucleus 1 0 0 0 0 3 6 12 8 7 6 14 0 1 0 – 7 3 0 0 0 0 0 2
Nuclear Periphery 1 0 2 0 1 1 8 7 3 4 8 12 0 0 0 – 9 7 7 0 4 2 4 2
Nucleolus 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 – 2 6 0 1 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 2 3 8 5 1 3 3 1 0 0 0 – 19 17 4 2 3 1 5 6
Vac/Vac Membrane 4 1 2 4 8 10 13 13 14 8 3 17 6 4 6 – 105 85 15 12 5 0 4 8
Unique Cell Count 211 82 136 164 193 293 368 413 253 309 240 287 165 194 179 260 163 247 101 161 97 227 214
Labelled Cell Count 243 91 148 186 208 367 451 489 313 401 296 382 172 197 182 348 219 247 101 161 97 227 214


Ambiguous, Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 3.3 3.4 3.5 3.5 3.1 2.7 3.2 3.1 2.7 2.9 2.7 3.8 3.9 3.8 4.8 5.4 5.9 3.7 4.1 4.8
Std Deviation (1e-4) 0.3 0.5 0.9 1.5 1.1 1.5 1.2 1.6 1.6 1.4 1.5 1.4 1.0 1.7 1.2 1.2 1.5 1.2 1.4 2.3 2.0
Intensity Change (Log2) – – – 0.05 0.03 -0.12 -0.36 -0.08 -0.16 -0.33 -0.22 -0.34 0.17 0.2 0.15 0.49 0.66 0.78 0.14 0.28 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 8.3 9.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7306 -0.5122 -0.913 -1.0791 -1.3018 -1.1411 -1.2899 -0.0147 -0.7213 -0.7647 -0.4581 -0.7233 0.1997 0.4465 0.3471 0.7656 0.5356 0.427
Actin 0.0239 0.001 0.006 0.0006 0.0029 0.0023 0.0069 0.0034 0.0051 0.0003 0.0002 0.0023 0.0224 0.0005 0.0063 0.0203 0.0078 0.0099
Bud 0.0005 0.0002 0.0002 0 0.0001 0 0.0001 0.0001 0.0001 0.0004 0 0 0.0004 0.0015 0.007 0.0003 0.001 0.0001
Bud Neck 0.001 0.0003 0.0011 0 0.0001 0.0009 0.0001 0.0005 0.0001 0 0 0.0002 0.0067 0.0001 0.0056 0.0061 0.0002 0.0005
Bud Periphery 0.0015 0.0004 0.0003 0 0.0005 0.0001 0.0002 0.0002 0.0002 0.0005 0 0 0.0008 0.0009 0.0034 0.0005 0.0082 0.0001
Bud Site 0.0036 0.0013 0.0003 0 0.0001 0.0001 0.0002 0.0006 0.0007 0.0001 0 0 0.0136 0.002 0.024 0.0012 0.0003 0.0001
Cell Periphery 0.0003 0.0004 0.0002 0 0.0001 0.0001 0.0002 0.0005 0.0001 0 0 0 0.0007 0.0001 0.0005 0.0006 0.0001 0.0001
Cytoplasm 0.0394 0.0725 0.0537 0.0333 0.0542 0.1106 0.0251 0.0113 0.0435 0.0095 0.0086 0.027 0.0421 0.0251 0.0328 0.0561 0.0598 0.0788
Cytoplasmic Foci 0.0368 0.008 0.0091 0.0045 0.0041 0.0076 0.0135 0.0023 0.0134 0.004 0.0034 0.0098 0.0097 0.0142 0.009 0.0151 0.01 0.0149
Eisosomes 0.0006 0.0009 0.0013 0.0003 0.0002 0.0002 0.0009 0.0023 0.0004 0.0005 0.0002 0.0002 0.0011 0.0004 0.0006 0.0035 0.0006 0.0003
Endoplasmic Reticulum 0.0399 0.0252 0.0331 0.0051 0.008 0.016 0.0108 0.0008 0.0066 0.0003 0 0.0011 0.0324 0.0013 0.0114 0.0248 0.0277 0.0195
Endosome 0.0356 0.0116 0.0237 0.0015 0.0158 0.006 0.0091 0.0019 0.0244 0.0003 0.0015 0.0031 0.0176 0.0062 0.0054 0.0133 0.0126 0.005
Golgi 0.0055 0.0004 0.004 0.0001 0.0016 0.0012 0.006 0.0011 0.0157 0.0001 0.0034 0.0004 0.0048 0.0003 0.0008 0.0107 0.008 0.0021
Lipid Particles 0.0215 0.0029 0.0136 0.0014 0.0044 0.0049 0.0166 0.0213 0.0253 0.0003 0.0824 0.001 0.008 0.0039 0.0024 0.0044 0.0145 0.006
Mitochondria 0.0046 0.0012 0.0123 0.0004 0.0058 0.0047 0.006 0.0218 0.0209 0.0004 0.0005 0.0002 0.0023 0.0003 0.0081 0.0059 0.0321 0.0019
None 0.6433 0.8136 0.7421 0.9413 0.8035 0.7956 0.8934 0.9159 0.7982 0.9751 0.8904 0.9491 0.8145 0.9227 0.8469 0.8107 0.7741 0.8194
Nuclear Periphery 0.0827 0.0443 0.0765 0.0063 0.0681 0.0272 0.0022 0.0011 0.028 0.0016 0 0.0013 0.0119 0.0029 0.0094 0.0074 0.017 0.0245
Nucleolus 0.0016 0.0009 0.0012 0.0003 0.0018 0.0008 0.0002 0.0032 0.0004 0.0003 0 0.0001 0.0006 0.0006 0.0016 0.0012 0.0007 0.0003
Nucleus 0.008 0.005 0.0069 0.0015 0.0105 0.0072 0.0005 0.0014 0.0034 0.0007 0 0.0006 0.0032 0.0015 0.0099 0.0023 0.0033 0.0035
Peroxisomes 0.0225 0.0002 0.0026 0.0002 0.0006 0.0067 0.0061 0.0024 0.0056 0.0005 0.0091 0.0006 0.0029 0.0005 0.006 0.0105 0.0151 0.0041
Punctate Nuclear 0.0162 0.0032 0.0048 0.0024 0.0052 0.0057 0.0008 0.0015 0.0045 0.0049 0.0002 0.0029 0.0016 0.0139 0.0068 0.003 0.0041 0.0069
Vacuole 0.0062 0.0032 0.0039 0.0004 0.0064 0.0011 0.0007 0.0043 0.0017 0.0002 0 0.0001 0.002 0.0007 0.0017 0.0015 0.0017 0.0009
Vacuole Periphery 0.005 0.003 0.0029 0.0002 0.0058 0.0011 0.0006 0.0022 0.0017 0.0001 0 0.0001 0.0005 0.0002 0.0004 0.0005 0.0011 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.1671 6.7792 10.0609 20.2691 17.2067 13.6428 9.7676 14.5728 17.2665 17.0141
Translational Efficiency 0.7589 1.0789 1.0581 0.5694 0.5119 1.1998 0.8098 0.6913 0.7612 0.6454

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1925 1530 1262 1544 2012 1837 149 646 3937 3367 1411 2190

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 558.32 688.03 824.81 754.70 630.87 668.78 947.81 747.03 595.40 677.53 837.80 752.44
Standard Deviation 305.74 83.99 93.58 94.72 66.24 90.48 90.23 94.57 221.95 88.11 100.60 94.74
Intensity Change Log 2 — 0.301379 0.562970 0.434811 — 0.084189 0.587255 0.243823 — 0.190239 0.575904 0.336646

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000071 0.000270 0.000301 0.000234 0.000085 0.000275 0.000482 0.000105 0.000078 0.000273 0.000320 0.000196
Bud Neck 0.019998 0.023167 0.005390 0.008823 0.021102 0.032575 0.001395 0.006636 0.020562 0.028300 0.004968 0.008178
Bud Site 0.007363 0.006177 0.005595 0.014778 0.002202 0.008844 0.001222 0.005182 0.004726 0.007632 0.005133 0.011947
Cell Periphery 0.000726 0.000140 0.000101 0.000129 0.000142 0.000168 0.000217 0.000485 0.000427 0.000155 0.000114 0.000234
Cytoplasm 0.482331* 0.363941* 0.293224* 0.417299* 0.431226* 0.362171* 0.206882* 0.379986* 0.456214* 0.362975* 0.284106* 0.406292*
Cytoplasmic Foci 0.167235 0.229321* 0.009968 0.022001 0.239911* 0.262645* 0.028834 0.042102 0.204376* 0.247502* 0.011961 0.027930
Eisosomes 0.000495 0.000120 0.000028 0.000048 0.000192 0.000190 0.000118 0.000192 0.000340 0.000158 0.000038 0.000091
Endoplasmic Reticulum 0.004139 0.002730 0.004921 0.002968 0.001796 0.001162 0.019278 0.001032 0.002942 0.001874 0.006437 0.002397
Endosome 0.004178 0.016563 0.002428 0.004326 0.010231 0.020736 0.008730 0.005568 0.007271 0.018839 0.003094 0.004693
Golgi 0.002378 0.003565 0.000075 0.000716 0.002454 0.005362 0.000121 0.001471 0.002417 0.004545 0.000080 0.000939
Lipid Particles 0.023316 0.016801 0.001727 0.001917 0.023217 0.018994 0.008828 0.002554 0.023265 0.017998 0.002477 0.002105
Mitochondria 0.009557 0.015807 0.000942 0.005141 0.007140 0.020755 0.000828 0.002164 0.008322 0.018506 0.000930 0.004263
Mitotic Spindle 0.000107 0.005025 0.005145 0.022123 0.000509 0.008468 0.000199 0.004639 0.000313 0.006903 0.004623 0.016966
None 0.018676 0.003808 0.007047 0.004034 0.003141 0.003395 0.003078 0.008222 0.010737 0.003583 0.006628 0.005269
Nuclear Periphery 0.002038 0.003224 0.021488 0.008645 0.001684 0.000963 0.056357 0.001651 0.001857 0.001991 0.025170 0.006582
Nuclear Periphery Foci 0.001412 0.001309 0.007044 0.004424 0.000536 0.000373 0.036425 0.001507 0.000964 0.000798 0.010146 0.003564
Nucleolus 0.002285 0.002980 0.002824 0.001237 0.001268 0.003038 0.005301 0.002762 0.001765 0.003012 0.003086 0.001687
Nucleus 0.233709* 0.236717* 0.608316* 0.411630* 0.215708* 0.180013* 0.504829* 0.485333* 0.224510* 0.205780* 0.597388* 0.433371*
Peroxisomes 0.003227 0.014113 0.001019 0.001387 0.006533 0.022597 0.000753 0.001110 0.004916 0.018742 0.000991 0.001305
Vacuole 0.015549 0.051853 0.022094 0.066883 0.029921 0.045183 0.115697 0.046055 0.022894 0.048214 0.031978 0.060739
Vacuole Periphery 0.001210 0.002370 0.000323 0.001257 0.001002 0.002096 0.000426 0.001242 0.001104 0.002221 0.000334 0.001253

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.92 -3.55 -5.90 0.50 1.11 -6.69 -6.97 -3.50 3.80 5.93 -9.62 -4.25 -6.49 1.79 2.05
Bud Neck -1.55 9.03 6.89 7.86 -2.18 -4.73 13.04 6.35 10.02 -3.17 -4.78 12.35 9.55 13.19 -2.49
Bud Site 1.07 1.26 -1.72 -2.69 -2.61 -6.25 1.00 -1.87 2.02 -2.06 -3.47 -0.17 -3.30 -1.04 -2.41
Cell Periphery 7.98 8.42 8.32 2.14 -0.61 -1.96 -4.23 -3.74 -3.33 -2.13 7.42 8.30 5.58 -1.93 -3.40
Cytoplasm 13.95 23.15 15.38 1.34 -7.43 8.22 14.00 6.74 0.99 -8.08 15.60 24.47 15.36 1.72 -9.34
Cytoplasmic Foci -9.13 41.34 38.12 36.17 -5.75 -3.16 32.12 35.42 37.03 -1.81 -8.69 59.58 53.88 55.06 -7.71
Eisosomes 12.17 15.11 14.28 9.17 -3.91 0.22 3.52 0.76 0.62 -0.63 10.47 18.79 13.45 5.21 -3.84
Endoplasmic Reticulum 2.02 -2.15 -0.29 -4.27 2.99 2.06 -7.16 -1.24 -3.36 6.99 2.87 -7.46 -2.05 -7.31 6.99
Endosome -10.31 3.40 0.61 10.07 -1.73 -7.14 -0.06 3.81 9.32 2.86 -11.99 7.42 4.85 14.71 -1.13
Golgi -2.46 13.28 6.90 6.53 -1.47 -5.44 10.43 2.32 5.86 -1.81 -5.88 16.46 6.77 9.86 -2.36
Lipid Particles 3.34 13.49 13.44 12.45 -0.62 2.27 7.89 13.54 13.87 5.59 3.90 18.59 19.21 18.84 1.55
Mitochondria -3.14 8.28 4.59 6.68 -2.39 -7.78 10.64 5.81 10.64 -1.47 -7.73 12.41 6.15 11.63 -2.64
Mitotic Spindle -4.34 -4.45 -7.56 -5.29 -4.70 -5.32 -0.23 -3.32 0.30 -3.26 -6.85 -4.30 -8.15 -4.47 -4.68
None 8.50 6.45 8.75 -0.20 3.10 -0.56 0.86 -3.94 -3.61 -4.33 7.83 3.98 6.31 -2.75 1.54
Nuclear Periphery -1.31 -6.79 -4.94 -3.83 3.96 1.97 -3.32 -1.65 -3.44 3.23 -0.32 -7.69 -5.41 -5.24 5.50
Nuclear Periphery Foci 0.24 -3.93 -3.37 -3.86 1.24 1.14 -3.94 -2.00 -2.15 3.59 0.65 -5.81 -4.36 -4.80 3.12
Nucleolus -1.44 -1.30 0.52 1.73 1.60 -3.27 -2.44 -2.03 0.31 0.79 -3.27 -2.74 -0.98 1.62 1.55
Nucleus -0.60 -40.99 -23.49 -20.44 16.52 5.33 -11.06 -20.73 -23.11 0.50 3.57 -44.65 -31.87 -32.46 15.58
Peroxisomes -10.19 3.93 5.83 12.25 -0.10 -10.79 10.90 9.69 15.28 -0.94 -14.61 7.08 10.69 19.23 -0.16
Vacuole -16.11 -7.63 -21.70 -13.13 -16.92 -7.31 -8.47 -8.84 -6.21 4.80 -16.49 -8.45 -22.36 -15.16 -13.34
Vacuole Periphery -3.93 5.24 1.78 5.18 -2.15 -4.67 7.43 0.00 2.31 -1.47 -5.97 8.04 1.36 5.60 -2.66
Standard name
Human Ortholog
Description Protein involved in regulation of mitotic exit; dephosphorylation target of Cdc14p in anaphase, which promotes timely rDNA segregation and allows mitotic progression; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nur1

Nur1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nur1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available