Standard name
Human Ortholog
Description Component of the U4/U6.U5 snRNP complex; involved in mRNA splicing via spliceosome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.06 0.09 0.15 0.25 0.17 0.27 0.21 0.15 0.14 0.15 0.16 0.17 0.25 0.26 0.11 0.1 0.11 0.06 0 0.07 0.06 0.06 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.13 0 0 0 0.45 0.11 0.22 0.45 0.41 0.5 0.52 0 0 0 0.13 0 0.06 0 0 0 0 0 0
Nucleus 0.73 0.77 0.87 0.75 0.61 0.6 0.57 0.58 0.56 0.54 0.54 0.51 0.73 0.65 0.62 0.71 0.82 0.72 0.7 0.75 0.66 0.65 0.54 0.45
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Vac/Vac Membrane 0.1 0.12 0.06 0.07 0.13 0.05 0.15 0.09 0.08 0.06 0.08 0.08 0.08 0.08 0.09 0.13 0.09 0.15 0.12 0.12 0.16 0.19 0.22 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 3 5 8 8 5 0 0 0 0 0 0 1 5 0 7 9 12
Bud Neck 0 1 0 0 0 0 1 2 1 5 0 0 0 0 0 0 1 0 0 2 0 0 2 6
Bud Site 0 0 0 0 0 1 1 0 7 3 6 11 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 20 17 10 34 60 34 108 76 56 63 55 80 30 118 111 7 9 10 13 10 8 17 25 33
Endoplasmic Reticulum 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 4 3 0 0 0 1 3 1
Endosome 0 4 0 8 10 1 10 7 1 7 2 3 1 15 12 0 0 1 3 1 0 1 6 5
Golgi 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 2 2 3 1 0 0 3 0
Mitochondria 35 34 1 8 4 93 45 82 168 186 186 261 6 10 17 8 3 6 4 3 3 4 11 17
Nucleus 136 210 95 169 145 124 233 211 210 243 201 256 126 312 270 44 77 68 164 228 88 205 225 195
Nuclear Periphery 1 0 0 1 0 0 1 1 1 2 1 4 0 0 2 0 1 1 1 0 0 0 2 1
Nucleolus 0 0 0 0 0 1 0 0 1 3 1 1 1 1 0 2 0 2 2 2 1 4 7 9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 2 0 9 8 2 7 3 5 0 1 0 1 1 0 6 8 4 9 18 30
Vac/Vac Membrane 18 33 6 16 31 10 61 33 29 25 30 42 13 37 38 8 8 14 27 35 21 59 90 104
Unique Cell Count 186 271 109 225 238 205 406 366 375 452 373 504 172 479 433 62 94 95 234 303 134 315 413 432
Labelled Cell Count 212 299 114 238 252 265 471 424 481 552 493 668 177 494 451 70 106 107 234 303 134 315 413 432


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.9 5.7 5.1 5.5 3.9 5.2 4.7 4.2 4.3 4.0 4.1 5.1 5.4 5.1 6.2 6.4 6.8 4.7 5.0 4.9
Std Deviation (1e-4) 0.9 1.1 1.0 1.1 1.2 1.1 3.7 1.4 1.3 1.3 1.1 1.2 1.0 1.6 1.3 1.5 1.5 1.7 1.2 1.6 1.2
Intensity Change (Log2) – – – -0.16 -0.06 -0.55 -0.12 -0.27 -0.45 -0.39 -0.49 -0.47 -0.17 -0.07 -0.15 0.12 0.16 0.26 -0.28 -0.19 -0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 3.5 1.8 3.8 2.8 1.5 1.3 1.5 1.8 1.9 3.5 3.7 0.4 0.1 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.3 0 0 0 0 0 0 0 0 0 0 0
Nucleus -2.5 -4.9 -4.9 -5.7 -5.6 -5.9 -6.4 -6.3 -7.0 -2.8 -4.5 -4.9 -2.6 -1.0 -2.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.6 2.1 -0.2 2.6 1.2 0.8 0 0.9 1.0 0.7 0.8 1.1 1.7 0.8 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0008 0.0856 -0.3333 0.0307 0.4637 -0.1361 0.2446 0.8999 0.6029 0.8349 0.5025 0.8612 2.1086 2.1875 1.4583 2.2241 1.5826 1.8627
Actin 0.0191 0.0001 0.0008 0.0619 0.0173 0.0001 0.0135 0 0.0067 0.0252 0.0053 0.0037 0.0381 0.0001 0.0003 0 0.0046 0.0013
Bud 0.0004 0.0001 0.0002 0.0011 0.0003 0 0.0002 0 0.0008 0.0006 0.0001 0.0002 0.0003 0.0009 0.0001 0 0.0001 0.0001
Bud Neck 0.0039 0.0003 0.0003 0.0056 0.0076 0.0014 0.0039 0.0001 0.0004 0.0004 0.001 0.0025 0.0144 0.0007 0.0007 0.0001 0.0007 0.0104
Bud Periphery 0.0006 0.0002 0.0001 0.0007 0.0004 0 0.0004 0 0.0003 0.0004 0.0003 0.0007 0.0005 0.0014 0.0001 0 0.0001 0.0002
Bud Site 0.0048 0.0003 0.0011 0.002 0.0025 0 0.0014 0.0002 0.0018 0.0032 0.0003 0.0003 0.0081 0.0039 0.0003 0 0.0005 0.0002
Cell Periphery 0.0003 0 0 0.0001 0.0002 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0005 0.0003 0 0 0 0
Cytoplasm 0.0211 0.0304 0.0256 0.0307 0.0744 0.0143 0.0293 0.0299 0.0433 0.0226 0.0282 0.0121 0.0522 0.0422 0.0429 0.0184 0.0242 0.0278
Cytoplasmic Foci 0.0114 0.0013 0.0021 0.0039 0.0087 0.0022 0.0074 0.0003 0.0013 0.0042 0.0017 0.0017 0.0066 0.0003 0.0008 0.0002 0.0162 0.0023
Eisosomes 0.0003 0 0 0.0003 0.0002 0 0.0002 0 0 0 0.0003 0.0001 0.001 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0051 0.0017 0.0007 0.0038 0.005 0.0014 0.0029 0.0012 0.0033 0.0006 0.0034 0.0009 0.0023 0.0037 0.0008 0.0001 0.0009 0.001
Endosome 0.0141 0.0039 0.0012 0.0037 0.0198 0.0005 0.0152 0.0007 0.0047 0.0028 0.0097 0.0057 0.0088 0.0013 0.0009 0 0.0066 0.0034
Golgi 0.0028 0.0001 0.0001 0.0021 0.0046 0 0.0024 0 0.0003 0.0064 0.0012 0.0037 0.0032 0 0 0 0.0018 0.0004
Lipid Particles 0.0101 0.0003 0.0002 0.0021 0.0224 0.0002 0.0098 0 0.0004 0.0003 0.0027 0.0033 0.0066 0 0.0001 0 0.0064 0.0007
Mitochondria 0.0025 0.0006 0.0005 0.0009 0.0032 0.0001 0.0016 0.0003 0.0006 0.0008 0.0079 0.0043 0.0014 0.0011 0.0004 0.0002 0.0004 0.001
None 0.0256 0.0848 0.0959 0.0369 0.0419 0.0412 0.0412 0.039 0.0628 0.0437 0.111 0.0045 0.2066 0.0053 0.0944 0.1203 0.1337 0.048
Nuclear Periphery 0.0322 0.0202 0.0126 0.0362 0.0641 0.0153 0.0119 0.0096 0.0095 0.0028 0.0703 0.0144 0.0232 0.0171 0.0114 0.0017 0.0096 0.0101
Nucleolus 0.0129 0.006 0.0071 0.0077 0.0198 0.0117 0.0088 0.004 0.0044 0.0025 0.0104 0.0116 0.0046 0.0045 0.0058 0.0031 0.0095 0.0095
Nucleus 0.7778 0.8199 0.8282 0.7275 0.5972 0.8703 0.8223 0.9076 0.84 0.8746 0.6977 0.8964 0.596 0.9038 0.8033 0.8498 0.6995 0.8512
Peroxisomes 0.0029 0.0001 0.0002 0.0014 0.0091 0 0.0026 0 0.0002 0.0005 0.0006 0.0013 0.0034 0 0 0 0.005 0.0013
Punctate Nuclear 0.0485 0.028 0.0225 0.0698 0.098 0.0407 0.0211 0.0058 0.0123 0.0079 0.0434 0.0311 0.021 0.0051 0.0363 0.0058 0.079 0.0301
Vacuole 0.0023 0.0016 0.0006 0.0007 0.0018 0.0003 0.0028 0.0008 0.0057 0.0002 0.002 0.0007 0.001 0.0073 0.001 0.0001 0.0009 0.0005
Vacuole Periphery 0.0014 0.0003 0.0002 0.0009 0.0014 0.0001 0.0008 0.0002 0.0011 0.0001 0.0022 0.0008 0.0005 0.0008 0.0002 0 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.5446 13.4889 5.0047 9.9131 6.1137 18.8512 11.5076 7.4792 14.1147 11.7325
Translational Efficiency 2.361 1.0125 2.5746 1.4985 2.9054 1.1147 1.5765 2.4015 1.0938 1.5373

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
836 854 230 1600 2154 2560 1314 1301 2990 3414 1544 2901

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 645.91 758.08 1003.81 866.70 677.27 757.85 755.50 869.72 668.50 757.91 792.49 868.05
Standard Deviation 74.00 96.23 110.95 119.10 77.31 91.51 127.65 121.38 77.68 92.72 153.36 120.13
Intensity Change Log 2 — 0.231017 0.636081 0.424200 — 0.162181 0.157701 0.360820 — 0.196194 0.411000 0.392107

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000046 0.000294 0.001415 0.000460 0.000061 0.000090 0.000518 0.003038 0.000056 0.000141 0.000652 0.001616
Bud Neck 0.004991 0.004929 0.006400 0.011890 0.005584 0.007679 0.004956 0.016208 0.005418 0.006991 0.005171 0.013826
Bud Site 0.000804 0.002424 0.001706 0.005993 0.001686 0.002225 0.001770 0.018177 0.001439 0.002275 0.001760 0.011457
Cell Periphery 0.000258 0.000149 0.000102 0.000116 0.000189 0.000126 0.000174 0.000321 0.000208 0.000132 0.000164 0.000208
Cytoplasm 0.180565* 0.190494* 0.004527 0.031606 0.208863* 0.140666* 0.032406 0.100004* 0.200951* 0.153130* 0.028253 0.062280
Cytoplasmic Foci 0.084653 0.035889 0.000118 0.000969 0.018092 0.011126 0.001171 0.007615 0.036702 0.017320 0.001014 0.003950
Eisosomes 0.000088 0.000043 0.000045 0.000019 0.000021 0.000017 0.000019 0.000037 0.000040 0.000023 0.000022 0.000028
Endoplasmic Reticulum 0.001224 0.003552 0.011995 0.007237 0.001137 0.001360 0.004879 0.005438 0.001161 0.001908 0.005939 0.006431
Endosome 0.002656 0.002540 0.000504 0.001894 0.000673 0.001105 0.000683 0.009378 0.001227 0.001464 0.000656 0.005250
Golgi 0.000883 0.000851 0.000077 0.000700 0.000097 0.000364 0.000102 0.003472 0.000317 0.000486 0.000098 0.001943
Lipid Particles 0.014172 0.003877 0.000463 0.000534 0.002177 0.001347 0.000272 0.001658 0.005531 0.001980 0.000300 0.001038
Mitochondria 0.004513 0.002703 0.001435 0.002613 0.002238 0.003066 0.001278 0.004156 0.002874 0.002976 0.001301 0.003305
Mitotic Spindle 0.000160 0.002738 0.000130 0.013439 0.001061 0.003092 0.002174 0.052305 0.000809 0.003004 0.001869 0.030869
None 0.013435 0.012747 0.001875 0.006298 0.006631 0.005746 0.011555 0.015362 0.008534 0.007498 0.010113 0.010363
Nuclear Periphery 0.001466 0.001374 0.000667 0.002507 0.002076 0.001528 0.000602 0.002413 0.001906 0.001490 0.000611 0.002465
Nuclear Periphery Foci 0.000457 0.001177 0.000331 0.000813 0.000302 0.000252 0.000450 0.000944 0.000346 0.000484 0.000432 0.000872
Nucleolus 0.005573 0.005314 0.002506 0.001288 0.001793 0.002306 0.002461 0.002465 0.002850 0.003058 0.002467 0.001816
Nucleus 0.674060* 0.715691* 0.958749* 0.901572* 0.743802* 0.811671* 0.931800* 0.728425* 0.724302* 0.787662* 0.935815* 0.823922*
Peroxisomes 0.004972 0.002859 0.000279 0.000178 0.000656 0.001459 0.000208 0.001821 0.001863 0.001809 0.000218 0.000915
Vacuole 0.004453 0.009656 0.006535 0.008477 0.002668 0.004234 0.002393 0.023478 0.003167 0.005590 0.003010 0.015204
Vacuole Periphery 0.000571 0.000698 0.000142 0.001398 0.000192 0.000540 0.000131 0.003282 0.000298 0.000579 0.000133 0.002243

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.02 -5.18 -17.08 -5.60 3.52 -0.82 -7.77 -3.89 -3.83 -2.74 -3.15 -9.34 -5.40 -4.95 -1.82
Bud Neck -0.14 -2.45 -7.45 -7.93 -6.02 -1.97 -0.33 -6.98 -5.32 -6.86 -1.94 -0.93 -10.06 -8.22 -9.25
Bud Site -4.42 -2.20 -4.36 -2.12 -1.76 -0.81 -0.65 -6.68 -6.40 -6.55 -1.83 -1.51 -7.40 -6.44 -6.64
Cell Periphery 4.21 4.74 6.52 4.47 0.85 2.41 0.70 -0.11 -1.56 -0.54 3.74 2.17 2.59 -0.49 0.84
Cytoplasm -0.93 23.46 19.59 19.47 -10.52 10.63 31.59 18.21 8.73 -10.67 8.64 36.72 30.61 22.23 -8.47
Cytoplasmic Foci 7.99 16.60 16.45 11.32 -2.56 3.97 12.73 9.49 6.57 -3.85 8.98 19.74 18.89 13.99 -2.97
Eisosomes 7.07 4.57 10.89 4.76 4.12 2.50 0.59 -11.37 -13.56 -11.11 7.58 6.54 1.00 -8.67 -6.89
Endoplasmic Reticulum -6.83 -7.90 -16.59 -9.03 3.33 -0.75 -10.61 -14.07 -16.70 -6.23 -3.13 -13.68 -19.53 -19.97 -4.06
Endosome 0.08 3.27 2.02 2.60 -2.36 -2.11 0.00 -4.32 -3.66 -4.24 -1.15 2.27 -3.30 -2.61 -4.57
Golgi 0.16 3.63 1.98 1.36 -1.64 -1.84 -0.11 -2.97 -2.23 -2.96 -1.10 3.20 -2.23 -1.32 -3.35
Lipid Particles 7.13 9.72 10.10 7.14 1.00 2.47 8.25 2.39 0.59 -2.66 7.48 12.10 10.44 5.04 -2.58
Mitochondria 2.63 3.99 3.73 0.84 -1.30 -1.24 1.54 -1.37 0.02 -3.63 -0.16 3.14 0.60 0.84 -3.56
Mitotic Spindle -2.82 -1.25 -5.93 -3.73 -5.78 -2.15 -1.87 -9.00 -8.39 -8.51 -3.08 -2.18 -10.46 -9.07 -9.44
None 0.45 7.16 4.58 3.89 -5.59 1.16 -3.04 -2.35 -3.07 0.55 1.33 -0.64 1.23 0.05 1.52
Nuclear Periphery 0.32 4.07 -6.43 -6.64 -8.67 1.91 5.59 -4.24 -7.83 -13.43 1.91 6.49 -5.86 -9.24 -15.06
Nuclear Periphery Foci -3.00 -0.17 -1.89 1.47 -1.55 -0.02 -1.30 -4.22 -5.18 -1.32 -1.56 -1.25 -4.02 -2.77 -1.14
Nucleolus 0.63 3.58 6.77 5.36 2.28 -1.87 -2.17 -1.75 -0.17 0.18 -0.35 1.51 3.76 4.06 2.11
Nucleus -2.72 -22.69 -18.66 -15.98 7.69 -8.71 -26.11 -0.97 5.83 18.91 -8.96 -32.94 -15.26 -6.75 17.10
Peroxisomes 2.32 7.34 7.96 5.26 1.78 -1.74 2.58 -0.99 1.24 -2.97 -0.02 7.46 6.65 3.72 -1.60
Vacuole -4.49 -3.22 -8.32 -3.90 -4.01 -3.08 -0.25 -14.20 -13.63 -14.15 -5.17 -1.56 -16.52 -14.60 -15.93
Vacuole Periphery -0.66 2.70 -0.46 -0.06 -1.98 -2.84 1.28 -2.58 -1.80 -2.72 -2.69 2.93 -2.76 -1.64 -3.41
Standard name
Human Ortholog
Description Component of the U4/U6.U5 snRNP complex; involved in mRNA splicing via spliceosome
Localization
Cell Percentages nucleus (74%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Snu23

Snu23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snu23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available